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rabbit monoclonal anti phosphorylated btk tyr 223 antibody  (Novus Biologicals)


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    Novus Biologicals rabbit monoclonal anti phosphorylated btk tyr 223 antibody
    Antiproliferative activity (A) and <t>BTK</t> autophosphorylation inhibitory effects (B) of tirabrutinib. (A) TMD8 or U-2932 cells were treated with vehicle or different concentrations of tirabrutinib and incubated for 72 h at 37°C, 5% CO 2 /95% air. After culturing, the growth inhibition rate (%) was calculated by measuring a luminescent signal proportional to the amount of intracellular ATP using the CellTiter-Glo Luminescent Cell Viability Assay. The growth inhibition rate in the tirabrutinib group is plotted as the mean of 3 or 4 treated cultures from each treatment group ± standard error for TMD8 or U-2932, respectively. (B) TMD8 or U-2932 cells were treated with vehicle or different concentrations of tirabrutinib and incubated for 4 h at 37°C, 5% CO 2 /95% air. Autophosphorylated BTK (p-BTK) and total BTK (BTK) proteins were detected by western blot analysis. The intensity of each western blot band shown in was determined. The ratio of <t>phosphorylated</t> to total BTK is described as the mean of three cases ± standard error in the bar chart. The symbol -∞ indicates the vehicle group. The curve was estimated by nonlinear regression analysis using a four-parameter logistic model. BTK, Bruton’s tyrosine kinase.
    Rabbit Monoclonal Anti Phosphorylated Btk Tyr 223 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phosphorylated btk tyr 223 antibody/product/Novus Biologicals
    Average 92 stars, based on 22 article reviews
    rabbit monoclonal anti phosphorylated btk tyr 223 antibody - by Bioz Stars, 2026-05
    92/100 stars

    Images

    1) Product Images from "Investigation of the anti-tumor mechanism of tirabrutinib, a highly selective Bruton’s tyrosine kinase inhibitor, by phosphoproteomics and transcriptomics"

    Article Title: Investigation of the anti-tumor mechanism of tirabrutinib, a highly selective Bruton’s tyrosine kinase inhibitor, by phosphoproteomics and transcriptomics

    Journal: PLOS ONE

    doi: 10.1371/journal.pone.0282166

    Antiproliferative activity (A) and BTK autophosphorylation inhibitory effects (B) of tirabrutinib. (A) TMD8 or U-2932 cells were treated with vehicle or different concentrations of tirabrutinib and incubated for 72 h at 37°C, 5% CO 2 /95% air. After culturing, the growth inhibition rate (%) was calculated by measuring a luminescent signal proportional to the amount of intracellular ATP using the CellTiter-Glo Luminescent Cell Viability Assay. The growth inhibition rate in the tirabrutinib group is plotted as the mean of 3 or 4 treated cultures from each treatment group ± standard error for TMD8 or U-2932, respectively. (B) TMD8 or U-2932 cells were treated with vehicle or different concentrations of tirabrutinib and incubated for 4 h at 37°C, 5% CO 2 /95% air. Autophosphorylated BTK (p-BTK) and total BTK (BTK) proteins were detected by western blot analysis. The intensity of each western blot band shown in was determined. The ratio of phosphorylated to total BTK is described as the mean of three cases ± standard error in the bar chart. The symbol -∞ indicates the vehicle group. The curve was estimated by nonlinear regression analysis using a four-parameter logistic model. BTK, Bruton’s tyrosine kinase.
    Figure Legend Snippet: Antiproliferative activity (A) and BTK autophosphorylation inhibitory effects (B) of tirabrutinib. (A) TMD8 or U-2932 cells were treated with vehicle or different concentrations of tirabrutinib and incubated for 72 h at 37°C, 5% CO 2 /95% air. After culturing, the growth inhibition rate (%) was calculated by measuring a luminescent signal proportional to the amount of intracellular ATP using the CellTiter-Glo Luminescent Cell Viability Assay. The growth inhibition rate in the tirabrutinib group is plotted as the mean of 3 or 4 treated cultures from each treatment group ± standard error for TMD8 or U-2932, respectively. (B) TMD8 or U-2932 cells were treated with vehicle or different concentrations of tirabrutinib and incubated for 4 h at 37°C, 5% CO 2 /95% air. Autophosphorylated BTK (p-BTK) and total BTK (BTK) proteins were detected by western blot analysis. The intensity of each western blot band shown in was determined. The ratio of phosphorylated to total BTK is described as the mean of three cases ± standard error in the bar chart. The symbol -∞ indicates the vehicle group. The curve was estimated by nonlinear regression analysis using a four-parameter logistic model. BTK, Bruton’s tyrosine kinase.

    Techniques Used: Activity Assay, Incubation, Inhibition, Cell Viability Assay, Western Blot



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    rabbit monoclonal anti phosphorylated btk tyr 223 antibody  (Novus Biologicals)
    92
    Novus Biologicals rabbit monoclonal anti phosphorylated btk tyr 223 antibody
    Antiproliferative activity (A) and <t>BTK</t> autophosphorylation inhibitory effects (B) of tirabrutinib. (A) TMD8 or U-2932 cells were treated with vehicle or different concentrations of tirabrutinib and incubated for 72 h at 37°C, 5% CO 2 /95% air. After culturing, the growth inhibition rate (%) was calculated by measuring a luminescent signal proportional to the amount of intracellular ATP using the CellTiter-Glo Luminescent Cell Viability Assay. The growth inhibition rate in the tirabrutinib group is plotted as the mean of 3 or 4 treated cultures from each treatment group ± standard error for TMD8 or U-2932, respectively. (B) TMD8 or U-2932 cells were treated with vehicle or different concentrations of tirabrutinib and incubated for 4 h at 37°C, 5% CO 2 /95% air. Autophosphorylated BTK (p-BTK) and total BTK (BTK) proteins were detected by western blot analysis. The intensity of each western blot band shown in was determined. The ratio of <t>phosphorylated</t> to total BTK is described as the mean of three cases ± standard error in the bar chart. The symbol -∞ indicates the vehicle group. The curve was estimated by nonlinear regression analysis using a four-parameter logistic model. BTK, Bruton’s tyrosine kinase.
    Rabbit Monoclonal Anti Phosphorylated Btk Tyr 223 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phosphorylated btk tyr 223 antibody/product/Novus Biologicals
    Average 92 stars, based on 1 article reviews
    rabbit monoclonal anti phosphorylated btk tyr 223 antibody - by Bioz Stars, 2026-05
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Antiproliferative activity (A) and BTK autophosphorylation inhibitory effects (B) of tirabrutinib. (A) TMD8 or U-2932 cells were treated with vehicle or different concentrations of tirabrutinib and incubated for 72 h at 37°C, 5% CO 2 /95% air. After culturing, the growth inhibition rate (%) was calculated by measuring a luminescent signal proportional to the amount of intracellular ATP using the CellTiter-Glo Luminescent Cell Viability Assay. The growth inhibition rate in the tirabrutinib group is plotted as the mean of 3 or 4 treated cultures from each treatment group ± standard error for TMD8 or U-2932, respectively. (B) TMD8 or U-2932 cells were treated with vehicle or different concentrations of tirabrutinib and incubated for 4 h at 37°C, 5% CO 2 /95% air. Autophosphorylated BTK (p-BTK) and total BTK (BTK) proteins were detected by western blot analysis. The intensity of each western blot band shown in was determined. The ratio of phosphorylated to total BTK is described as the mean of three cases ± standard error in the bar chart. The symbol -∞ indicates the vehicle group. The curve was estimated by nonlinear regression analysis using a four-parameter logistic model. BTK, Bruton’s tyrosine kinase.

    Journal: PLOS ONE

    Article Title: Investigation of the anti-tumor mechanism of tirabrutinib, a highly selective Bruton’s tyrosine kinase inhibitor, by phosphoproteomics and transcriptomics

    doi: 10.1371/journal.pone.0282166

    Figure Lengend Snippet: Antiproliferative activity (A) and BTK autophosphorylation inhibitory effects (B) of tirabrutinib. (A) TMD8 or U-2932 cells were treated with vehicle or different concentrations of tirabrutinib and incubated for 72 h at 37°C, 5% CO 2 /95% air. After culturing, the growth inhibition rate (%) was calculated by measuring a luminescent signal proportional to the amount of intracellular ATP using the CellTiter-Glo Luminescent Cell Viability Assay. The growth inhibition rate in the tirabrutinib group is plotted as the mean of 3 or 4 treated cultures from each treatment group ± standard error for TMD8 or U-2932, respectively. (B) TMD8 or U-2932 cells were treated with vehicle or different concentrations of tirabrutinib and incubated for 4 h at 37°C, 5% CO 2 /95% air. Autophosphorylated BTK (p-BTK) and total BTK (BTK) proteins were detected by western blot analysis. The intensity of each western blot band shown in was determined. The ratio of phosphorylated to total BTK is described as the mean of three cases ± standard error in the bar chart. The symbol -∞ indicates the vehicle group. The curve was estimated by nonlinear regression analysis using a four-parameter logistic model. BTK, Bruton’s tyrosine kinase.

    Article Snippet: Rabbit monoclonal anti- phosphorylated BTK (Tyr-223) antibody (p-BTK; Novus Biologicals, #NB100-79907), rabbit monoclonal BTK antibody (Cell Signaling Technology, Inc., #3533), rabbit polyclonal phosphorylated AKT (Ser-473) antibody (p-AKT; Cell Signaling Technology, Inc., #9271), rabbit monoclonal AKT (pan) (C67E7) antibody (Cell Signaling Technology, Inc., #4691), rabbit polyclonal phosphorylated p44/42 MAPK (ERK1/2) (Thr-202/Tyr-204) antibody (p-ERK1/2; Cell Signaling Technology, Inc., #9101), rabbit monoclonal p44/42 MAPK (ERK1/2) (137F5) antibody (Cell Signaling Technology, Inc., #4695), rabbit monoclonal phosphorylated PLCγ2 (Tyr-759) (E9E9Y) antibody (p-PLCγ2; Cell Signaling Technology, Inc., #50535), rabbit monoclonal PLCγ2 (E5U4T) antibody (Cell Signaling Technology, Inc., #55512), mouse monoclonal phosphorylated IκBα (Ser-32/36) (5A5) antibody (p-IκBα; Cell Signaling Technology, Inc., #9246), rabbit monoclonal IκBα (44D4) antibody (Cell Signaling Technology, Inc., #4812), rabbit polyclonal phosphorylated PKCβ (phospho-Thr-641) antibody (p-PKCβ; Signalway Antibody LLC, #11172), rabbit monoclonal IRF4 (D9P5H) antibody (Cell Signaling Technology, Inc., #15106), rabbit monoclonal BCL6 (D65C10) antibody (Cell Signaling Technology, Inc., #5650), rabbit monoclonal c-MYC (D84C12) antibody (Cell Signaling Technology, Inc., #5605), and rabbit monoclonal GAPDH (14C10) antibody (Cell Signaling Technology, Inc., #2118) were used.

    Techniques: Activity Assay, Incubation, Inhibition, Cell Viability Assay, Western Blot