Journal: iScience

Article Title: TERC promotes non-small cell lung cancer progression by facilitating the nuclear localization of TERT

doi: 10.1016/j.isci.2024.109869

Figure Lengend Snippet: Knockdown of TERC inhibits the nuclear functions of TERT (A) Relative telomere repeat number in NSCLC cells. Cellular DNA was extracted from A549, H322, H460, and H1299 cells without (ctrl) or with TERC knockdown (TERC-si1, TERC-si2), or treated with 10 μM BIBR1532, and qPCR was performed for each extract to obtain the relative telomere repeat number as described previously, using 36B4 as the single-copy gene control. (B) Binding of TERT at the indicated genomic regions. ChIP was performed with TERT antibody, or IgG as control, and qPCR was run with primers specific for the tested target regions in A549 cells without or with TERC knockdown, or treated with 10 μM BIBR1532. Reported values correspond to the signal for each site relative to that of the IgG control. (C) Gene expression analysis of pre-tRNA, pre-rRNA, rRNA, as well as c-Myc, cyclin D1, and VEGF mRNA transcripts in A549 cells. RT-qPCR was performed, and U6 served as the control for pre-tRNA, pre-rRNA, and rRNA transcripts, while β-actin served as the control for mRNA transcripts. Data in (A–C) were presented as mean ± SD ( n ≥ 2), and p values between the control and the TERC-si1/2 groups were calculated by Student’s t -test. (D) Gene expression analysis in xenograft tumors from A549 cells without (control) or with TERC stably knocked down (TERC-sh). Data were presented as individual dots and mean ± SD ( n = 4), and p values were determined by Student’s t -test. (E) Protein expression of cyclin D1. Western blotting was used to detect cyclin D1 levels in A549 cells. Histone H3 served as the loading control.

Article Snippet: Rabbit monoclonal (92G2) anti-Cyclin D1 , Cell Signaling Technology , Cat#2978; RRID: AB_2259616.

Techniques: Binding Assay, Expressing, Quantitative RT-PCR, Stable Transfection, Western Blot

Journal: iScience

Article Title: TERC promotes non-small cell lung cancer progression by facilitating the nuclear localization of TERT

doi: 10.1016/j.isci.2024.109869

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal (92G2) anti-Cyclin D1 , Cell Signaling Technology , Cat#2978; RRID: AB_2259616.

Techniques: Virus, Recombinant, Modification, Immunoprecipitation, Transfection, Membrane, DNA Purification, SYBR Green Assay, Isolation, Staining, Methylation, Software

Journal: Cell Reports Medicine

Article Title: Co-targeting RANK pathway treats and prevents acquired resistance to CDK4/6 inhibitors in luminal breast cancer

doi: 10.1016/j.xcrm.2023.101120

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti Cyclin D1 (92G2) , Cell Signaling , Cat#2978; RRID:AB_2259616.

Techniques: Recombinant, shRNA, Blocking Assay, Alamar Blue Assay, Flow Cytometry, Isolation, Bicinchoninic Acid Protein Assay, TUNEL Assay, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Software