rabbit mabs  (R&D Systems)

Journal: Journal of Virology

Article Title: N-Glycans on the Nipah Virus Attachment Glycoprotein Modulate Fusion and Viral Entry as They Protect against Antibody Neutralization

doi: 10.1128/JVI.01304-12

Figure Lengend Snippet: Synergistic and antagonistic effects of multiple NiV-G N-glycans in combination on cell-cell fusion. (A) Relative levels of 293T cell surface expression (HA), binding to MAbs (MAb26, MAb45, and MAb213), binding to soluble ephrinB2 receptor, and cell-cell

Article Snippet: The binding of anti-NiV-F antiserum 834 to surface NiV-F and the binding of the anti-NiV-G specific rabbit MAbs (MAb26, MAb45, or MAb213) or mouse anti-hemagglutinin (anti-HA) MAb or soluble ephinB2 fused to human Fc (B2-hFc; R & D Systems, Minneapolis, MN) to cell surface NiV-G were measured by flow cytometry on NiV-F/G-transfected cells.

Techniques: Expressing, Binding Assay

Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

Article Title: Induction of HLA-DP4-restricted anti-Survivin Th1 and Th2 responses using an artificial antigen-presenting cell

doi: 10.1158/1078-0432.CCR-10-3083

Figure Lengend Snippet: Both Survivin-specific Th1 and Th2 cells are able to recognize Survivin-expressing tumors in a DP4-restricted manner (A) The renal cancer cell lines, A-498, ACHN, and A-704, are endogenously defective for HLA-DP and DR expression. These cells were retrovirally transduced with HLA-DP4. HLADP and DR expression on parental and DP4-transduced was analyzed by flow cytometry analysis using specific mAbs. (B) Survivin protein expression by renal cancer cell lines, A-498, ACHN, and A-704, was analyzed by immunoblot analysis using specific mAb. (C) DP4-restricted Survivin #90 -specific CD4 + T cells recognize DP4 + Survivin + tumor cells. Survivin #90 -specific CD4 + T cells (Patient A1) were incubated with DP4-transduced or parental renal cancer cell lines, A-498, ACHN, and A-704 in IFN-γ and IL-4 ELISPOT. Similar results for IFN-γ secretion were obtained with Survivin #90 -specific CD4 + T cells (Patient B1) (data not shown).

Article Snippet: Briefly, duplicate PVDF membranes were loaded with 30 μg cell lysates of three renal cancer cell lines, A-498, ACHN, and A-704, and were probed with rabbit anti-Survivin mAb (R & D Systems) or mouse anti-α-tubulin mAb (Sigma, St. Louis, MO).

Techniques: Expressing, Transduction, Flow Cytometry, Cytometry, Incubation, Enzyme-linked Immunospot

Journal: BMC Molecular and Cell Biology

Article Title: The Glypican proteoglycans show intrinsic interactions with Wnt-3a in human prostate cancer cells that are not always associated with cascade activation

doi: 10.1186/s12860-021-00361-x

Figure Lengend Snippet: Immunoblot assays of RWPE-1, PC-3, DU-145, and LNCaP cells, evaluating Wnt-3a, and β-catenin. LNCaP cells were not analyzed using the Wnt-3a antibody. a Immunoblot results for Wnt-3a, cellular, cytoplasmic and nuclear β-catenin for RWPE-1, PC-3, DU-145, and LNCaP. Housekeeping proteins such as GAPDH and Lamin A were also investigated. b Densitometric analyses of the immunoblots, where data are normalized against GAPDH, except for nuclear β-catenin, which was normalized against Lamin A. Data are plotted as the mean ± s.e.m., and two independent experiments were performed. Prostate cancer cells were compared to RWPE-1 cells using Dunnett’s test. * p

Article Snippet: Polyclonal goat anti-glypican-1 (AF4519), polyclonal goat anti-glypican-5 (AF2607), monoclonal rat anti-Wnt-3a (MAB 9025), and monoclonal rabbit anti-β-catenin antibodies (MAB13291) were purchased from R & D Systems (Minneapolis, USA); monoclonal rabbit anti-GAPDH (ab128915) was purchased from ABCAM (Cambridge, MA, USA); polyclonal rabbit anti-lamin A (sc-20,680) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA); and Alexa Fluor™594 Phalloidin (A12381) was obtained from Molecular Probes (Eugene, OR, USA).

Techniques: Western Blot

Journal: BMC Molecular and Cell Biology

Article Title: The Glypican proteoglycans show intrinsic interactions with Wnt-3a in human prostate cancer cells that are not always associated with cascade activation

doi: 10.1186/s12860-021-00361-x

Figure Lengend Snippet: Flow cytometry analysis of GPCs, Wnt-3a and β-catenin in prostate cancer cell lines. a GPCs, b Wnt-3a, and ( c ) β-catenin were analyzed in RWPE-1, PC-3, DU-145 and LNCaP. GPC1 was assessed in RWPE-1 (blue), PC-3 (green), and DU-145 cells (red), while GPC5 was assessed in LNCaP cells (orange). (1) The positively stained cells for each protein were quantified and plotted. (2) The histograms reveal the distributions of unstained control (unfilled) and stained experimental assessments (filled) of GPCs, Wnt-3a, and β-catenin. Data are plotted as the mean ± s.e.m. Prostate cancer cells were compared to RWPE-1 cells using Dunnett’s test. ( n = 2–8). * p

Article Snippet: Polyclonal goat anti-glypican-1 (AF4519), polyclonal goat anti-glypican-5 (AF2607), monoclonal rat anti-Wnt-3a (MAB 9025), and monoclonal rabbit anti-β-catenin antibodies (MAB13291) were purchased from R & D Systems (Minneapolis, USA); monoclonal rabbit anti-GAPDH (ab128915) was purchased from ABCAM (Cambridge, MA, USA); polyclonal rabbit anti-lamin A (sc-20,680) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA); and Alexa Fluor™594 Phalloidin (A12381) was obtained from Molecular Probes (Eugene, OR, USA).

Techniques: Flow Cytometry, Staining

Journal: BMC Molecular and Cell Biology

Article Title: The Glypican proteoglycans show intrinsic interactions with Wnt-3a in human prostate cancer cells that are not always associated with cascade activation

doi: 10.1186/s12860-021-00361-x

Figure Lengend Snippet: β-catenin mRNA levels in RWPE-1, PC-3, DU-145, and LNCaP cells. Gene expression levels were quantified using RT-qPCR and normalized to the levels of GAPDH expression. Data are plotted as the mean ± s.e.m. of the 2 -ΔCT values, and three independent experiments were performed. Expression levels in prostate cancer cells were compared with those in the normal prostate cell line (RWPE-1) using Dunnett’s test. * p

Article Snippet: Polyclonal goat anti-glypican-1 (AF4519), polyclonal goat anti-glypican-5 (AF2607), monoclonal rat anti-Wnt-3a (MAB 9025), and monoclonal rabbit anti-β-catenin antibodies (MAB13291) were purchased from R & D Systems (Minneapolis, USA); monoclonal rabbit anti-GAPDH (ab128915) was purchased from ABCAM (Cambridge, MA, USA); polyclonal rabbit anti-lamin A (sc-20,680) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA); and Alexa Fluor™594 Phalloidin (A12381) was obtained from Molecular Probes (Eugene, OR, USA).

Techniques: Expressing, Quantitative RT-PCR

Journal: Journal of Applied Physiology

Article Title: Exercise effects on γ3-AMPK activity, phosphorylation of Akt2 and AS160, and insulin-stimulated glucose uptake in insulin-resistant rat skeletal muscle

doi: 10.1152/japplphysiol.00428.2019

Figure Lengend Snippet: A–D : phosphorylated Akt Thr308 /Akt, Akt2 Thr309 /Akt2, Akt Ser473 /Akt, and Akt2 Ser474 /Akt2 at 3 h postexercise. Data were analyzed by two-way ANOVA. * P

Article Snippet: After Akt2-IP, we immunoblotted using an antibody that has been validated by the vendor to recognize pThr309 on Akt2 (Cell Signaling Technology #13038).).

Techniques: