rabbit antirat secondary antibodies  (Vector Laboratories)


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    Name:
    Biotinylated Rabbit Anti Rat IgG Antibody
    Description:
    Biotinylated Rabbit Anti Rat IgG Antibody is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots Biotinylated Rabbit Anti Rat IgG H L is supplied in lyophilized form and can be reconstituted with 1 ml water With some exceptions the recommended dilution for most applications is 1 200 H L indicates the antibody recognizes both heavy and light chains This antibody is included in the VECTASTAIN ABC kits
    Catalog Number:
    BA-4000
    Price:
    None
    Category:
    Antibodies
    Reactivity:
    Rat
    Size:
    1 5 mg
    Host:
    Rabbit
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    Structured Review

    Vector Laboratories rabbit antirat secondary antibodies
    Biotinylated Rabbit Anti Rat IgG Antibody
    Biotinylated Rabbit Anti Rat IgG Antibody is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots Biotinylated Rabbit Anti Rat IgG H L is supplied in lyophilized form and can be reconstituted with 1 ml water With some exceptions the recommended dilution for most applications is 1 200 H L indicates the antibody recognizes both heavy and light chains This antibody is included in the VECTASTAIN ABC kits
    https://www.bioz.com/result/rabbit antirat secondary antibodies/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit antirat secondary antibodies - by Bioz Stars, 2021-06
    97/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Abnormalities of Thymic Stroma may Contribute to Immune Dysregulation in Murine Models of Leaky Severe Combined Immunodeficiency
    Article Snippet: After washes, single immunostains were revealed using the ChemMATE Envision Rabbit/Mouse (DAKO Cytomation, Glostrup, Denmark) or NovoLinkTM Polymer Detection System (NovocastraTM Laboratories Ltd, Newcastle Upon Tyne, United Kingdom) followed by diaminobenzidine (DAB) as chromogen and Hematoxylin as counterstain. .. The same procedure was applied to double immunofluorescence stainings prior to the incubation with a secondary swine anti-rabbit FITC-conjugated antibody (1:30; DAKO Cytomation, Glostrup, Denmark) for CK5 and a rabbit anti-rat biotinylated antibody (1:200; Vector Laboratories, Burlingame, CA, USA) followed by Streptavidin–Texas Red (1:100; Southern Biotechnology Associates, Birmingham, AL, USA) for CK8. ..

    Article Title: ID family protein expression and regulation in hypoxic pulmonary hypertension
    Article Snippet: .. Immunofluorescence was performed on formalin-fixed, paraffin-embedded lung sections with the ID1–3 antibodies outlined above and detected with a rabbit IgG-specific biotin-conjugated secondary antibody, followed by streptavidin-conjugated HRP (ABC kit, Vector Laboratories). .. Cy3-labeled tyramide signal amplification was used to amplify the HRP signal (Perkin Elmer, NEL744), according to the manufacturers' instructions.

    Incubation:

    Article Title: Abnormalities of Thymic Stroma may Contribute to Immune Dysregulation in Murine Models of Leaky Severe Combined Immunodeficiency
    Article Snippet: After washes, single immunostains were revealed using the ChemMATE Envision Rabbit/Mouse (DAKO Cytomation, Glostrup, Denmark) or NovoLinkTM Polymer Detection System (NovocastraTM Laboratories Ltd, Newcastle Upon Tyne, United Kingdom) followed by diaminobenzidine (DAB) as chromogen and Hematoxylin as counterstain. .. The same procedure was applied to double immunofluorescence stainings prior to the incubation with a secondary swine anti-rabbit FITC-conjugated antibody (1:30; DAKO Cytomation, Glostrup, Denmark) for CK5 and a rabbit anti-rat biotinylated antibody (1:200; Vector Laboratories, Burlingame, CA, USA) followed by Streptavidin–Texas Red (1:100; Southern Biotechnology Associates, Birmingham, AL, USA) for CK8. ..

    Article Title: Macrophage lipoprotein lipase modulates the development of atherosclerosis but not adiposity
    Article Snippet: Cross-sections of aortic roots were incubated with primary antibodies for mouse MOMA-2 (1:600; Accurate Chemical and Scientific) overnight at 4°C to investigate macrophage infiltration in the atherosclerotic lesion. .. After washing, sections were incubated with biotinylated anti-rat antibody for 1 h at room temperature, and then with avidin-biotin peroxidase complex (Vector Labs) for 30 min. Lastly, sections were developed with 3,3′-diaminobenzidine tetrahydrochloride (DAB) (Sigma), and counterstained with hematoxylin. .. The adipose tissue of mice fed a WTD for 12 weeks was fixed with 10% neutral-buffered formalin, embedded in paraffin, and sections were stained with hematoxylin and eosin.

    Article Title: Immunohistochemical analysis of macroautophagy
    Article Snippet: Subsequently, the primary antibody was applied overnight in a damp box, followed by 2 × 5 min immersion in staining buffer. .. Next, the slides were incubated for 30 min with biotinylated anti-rabbit or anti-mouse immunoglobulins (Vectastain ABC Kit, Vector Laboratories), 1:200 diluted in staining buffer. .. In case the primary antibody was already biotinylated, blocking with normal serum and incubation with biotinylated secondary antibodies were omitted.

    Article Title: Melatonin MT1 and MT2 Receptors in the Ram Reproductive Tract
    Article Snippet: The sections were then washed in PBS pH 7.4 and incubated in normal horse serum as a blocking reagent for 40 min, followed by overnight incubation at 4 °C with the specific primary antibody (rabbit polyclonal antibody against the MT1 receptor (RRID:AB_1241048, GeneTex, Irvine, CA, USA) and rabbit polyclonal antibody against the MT2 receptor (RRID:AB_1619198, Acris Antibodies GmbH, Herford, Germany), both at a dilution of 1:50 in PBS. .. Subsequently, the slides were incubated with biotinylated anti-rabbit antiserum (Vector Laboratories) for 30 min. An avidin-biotin-peroxidase complex (Vector Laboratories) was then applied for 40 min. .. The binding sites of the primary antibodies were visualized by diaminobenzidine (DAB) and peroxidase solution (0.12 g DAB in 240 mL of PBS pH 7.4 containing 3% H2 O2 ) for 7 min.

    Formalin-fixed Paraffin-Embedded:

    Article Title: ID family protein expression and regulation in hypoxic pulmonary hypertension
    Article Snippet: .. Immunofluorescence was performed on formalin-fixed, paraffin-embedded lung sections with the ID1–3 antibodies outlined above and detected with a rabbit IgG-specific biotin-conjugated secondary antibody, followed by streptavidin-conjugated HRP (ABC kit, Vector Laboratories). .. Cy3-labeled tyramide signal amplification was used to amplify the HRP signal (Perkin Elmer, NEL744), according to the manufacturers' instructions.

    Labeling:

    Article Title: Endocytosis of a Glycosylphosphatidylinositol-anchored Protein via Clathrin-coated Vesicles, Sorting by Default in Endosomes, and Exocytosis via RAB11-positive Carriers V⃞
    Article Snippet: For double labeling (Figures , inset; ), the first antibody was generally detected by PAG-10 or PAG-6; after a block with a rabbit normal IgG (1:400), the second antibody was detected by PAG-10 or PAG-15. .. In one case ( , inset), clathrin was first labeled with rabbit anti-TbCLH antibodies and PAG-6, whereas VSG was subsequently detected using biotinylated rabbit anti-VSG IgG followed by a monoclonal mouse antibiotin antibody (1:100; Vector Laboratories, Burlingame, CA) and goat anti-mouse IgG-18-nm gold conjugates (1:20; Jackson Immunoresearch Laboratories, West Grove, PA). .. After labeling, the cryosections were embedded in 1.4% methylcellulose, 0.3% uranyl acetate.

    Avidin-Biotin Assay:

    Article Title: Anti-α4β1 Integrin Antibodies Attenuated Brain Inflammatory Changes in a Mouse Model of Alzheimer’s Disease
    Article Snippet: All mouse cytokine ELISA kits were obtained from R & D Systems (Minneapolis, MN). .. Elite Vectastain ABC avidin and biotin kits, biotinylated anti-rabbit, anti-mouse, and anti-rat antibodies and the Vector VIP kits were from Vector Laboratories Inc. (Burlingame, CA). .. Anti-CD68 was obtained from Serotec (Raleigh, NC).

    Article Title: Macrophage lipoprotein lipase modulates the development of atherosclerosis but not adiposity
    Article Snippet: Cross-sections of aortic roots were incubated with primary antibodies for mouse MOMA-2 (1:600; Accurate Chemical and Scientific) overnight at 4°C to investigate macrophage infiltration in the atherosclerotic lesion. .. After washing, sections were incubated with biotinylated anti-rat antibody for 1 h at room temperature, and then with avidin-biotin peroxidase complex (Vector Labs) for 30 min. Lastly, sections were developed with 3,3′-diaminobenzidine tetrahydrochloride (DAB) (Sigma), and counterstained with hematoxylin. .. The adipose tissue of mice fed a WTD for 12 weeks was fixed with 10% neutral-buffered formalin, embedded in paraffin, and sections were stained with hematoxylin and eosin.

    Article Title: Melatonin MT1 and MT2 Receptors in the Ram Reproductive Tract
    Article Snippet: The sections were then washed in PBS pH 7.4 and incubated in normal horse serum as a blocking reagent for 40 min, followed by overnight incubation at 4 °C with the specific primary antibody (rabbit polyclonal antibody against the MT1 receptor (RRID:AB_1241048, GeneTex, Irvine, CA, USA) and rabbit polyclonal antibody against the MT2 receptor (RRID:AB_1619198, Acris Antibodies GmbH, Herford, Germany), both at a dilution of 1:50 in PBS. .. Subsequently, the slides were incubated with biotinylated anti-rabbit antiserum (Vector Laboratories) for 30 min. An avidin-biotin-peroxidase complex (Vector Laboratories) was then applied for 40 min. .. The binding sites of the primary antibodies were visualized by diaminobenzidine (DAB) and peroxidase solution (0.12 g DAB in 240 mL of PBS pH 7.4 containing 3% H2 O2 ) for 7 min.

    Plasmid Preparation:

    Article Title: Anti-α4β1 Integrin Antibodies Attenuated Brain Inflammatory Changes in a Mouse Model of Alzheimer’s Disease
    Article Snippet: All mouse cytokine ELISA kits were obtained from R & D Systems (Minneapolis, MN). .. Elite Vectastain ABC avidin and biotin kits, biotinylated anti-rabbit, anti-mouse, and anti-rat antibodies and the Vector VIP kits were from Vector Laboratories Inc. (Burlingame, CA). .. Anti-CD68 was obtained from Serotec (Raleigh, NC).

    Staining:

    Article Title: Immunohistochemical analysis of macroautophagy
    Article Snippet: Subsequently, the primary antibody was applied overnight in a damp box, followed by 2 × 5 min immersion in staining buffer. .. Next, the slides were incubated for 30 min with biotinylated anti-rabbit or anti-mouse immunoglobulins (Vectastain ABC Kit, Vector Laboratories), 1:200 diluted in staining buffer. .. In case the primary antibody was already biotinylated, blocking with normal serum and incubation with biotinylated secondary antibodies were omitted.

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  • 97
    Vector Laboratories rabbit antirat igg biotinylated secondary antibody
    Rabbit Antirat Igg Biotinylated Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit antirat igg biotinylated secondary antibody/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit antirat igg biotinylated secondary antibody - by Bioz Stars, 2021-06
    97/100 stars
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    94
    Vector Laboratories rabbit antirat secondary antibody
    Rabbit Antirat Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit antirat secondary antibody/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit antirat secondary antibody - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

    97
    Vector Laboratories rabbit antirat igg
    Rabbit Antirat Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit antirat igg/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit antirat igg - by Bioz Stars, 2021-06
    97/100 stars
      Buy from Supplier

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