rabbit antirat igg biotinylated secondary antibody  (Vector Laboratories)


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    Name:
    Biotinylated Rabbit Anti Rat IgG Antibody
    Description:
    Biotinylated Rabbit Anti Rat IgG Antibody is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots Biotinylated Rabbit Anti Rat IgG H L is supplied in lyophilized form and can be reconstituted with 1 ml water With some exceptions the recommended dilution for most applications is 1 200 H L indicates the antibody recognizes both heavy and light chains This antibody is included in the VECTASTAIN ABC kits
    Catalog Number:
    ba-4000
    Price:
    None
    Host:
    Rabbit
    Size:
    1 5 mg
    Category:
    Antibodies
    Reactivity:
    Rat
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    Structured Review

    Vector Laboratories rabbit antirat igg biotinylated secondary antibody
    Biotinylated Rabbit Anti Rat IgG Antibody
    Biotinylated Rabbit Anti Rat IgG Antibody is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots Biotinylated Rabbit Anti Rat IgG H L is supplied in lyophilized form and can be reconstituted with 1 ml water With some exceptions the recommended dilution for most applications is 1 200 H L indicates the antibody recognizes both heavy and light chains This antibody is included in the VECTASTAIN ABC kits
    https://www.bioz.com/result/rabbit antirat igg biotinylated secondary antibody/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit antirat igg biotinylated secondary antibody - by Bioz Stars, 2021-01
    97/100 stars

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    Related Articles

    Next-Generation Sequencing:

    Article Title: Fluorescent Arc/Arg3.1 indicator mice: a versatile tool to study brain activity changes in vitro and in vivo
    Article Snippet: .. Briefly, slices were blocked with 10% NGS containing 0.1% Triton X-100 antibody in PBS for 1 hr and reacted with either anti-Arc (Arg3.1 antiserum, provided by Dietmar Kuhl, dilution 1:1,500) or anti-GFP (Molecular Probes, Cat# , dilution 1:2,000) rabbit antibody in 1% NGS containing PBS overnight at 4 C. After reaction with biotinylated anti-rabbit antibody (Vector laboratory, dilution 1:200), immunoreactions were visualized with the ABC elite kit (Vector laboratory) and DAB development procedure. .. Two month-old male Tg Arc/Arg3.1-d4EGFP mice (line 11; 21–24 g) received p-MPPI by i.p. injection at a dose 8.5 mg per kg between 8 and 9 a.m. (3 mice were used per experimental group); control mice were injected at the same time with saline.

    Avidin-Biotin Assay:

    Article Title: Macrophage lipoprotein lipase modulates the development of atherosclerosis but not adiposity
    Article Snippet: .. After washing, sections were incubated with biotinylated anti-rat antibody for 1 h at room temperature, and then with avidin-biotin peroxidase complex (Vector Labs) for 30 min. Lastly, sections were developed with 3,3′-diaminobenzidine tetrahydrochloride (DAB) (Sigma), and counterstained with hematoxylin. .. The adipose tissue of mice fed a WTD for 12 weeks was fixed with 10% neutral-buffered formalin, embedded in paraffin, and sections were stained with hematoxylin and eosin.

    Article Title: Anti-α4β1 Integrin Antibodies Attenuated Brain Inflammatory Changes in a Mouse Model of Alzheimer’s Disease
    Article Snippet: .. Elite Vectastain ABC avidin and biotin kits, biotinylated anti-rabbit, anti-mouse, and anti-rat antibodies and the Vector VIP kits were from Vector Laboratories Inc. (Burlingame, CA). .. Anti-CD68 was obtained from Serotec (Raleigh, NC).

    Article Title: Identification of a Novel UT-B Urea Transporter in Human Urothelial Cancer
    Article Snippet: .. After a PBS wash, tissues were incubated with biotinylated anti-rabbit antibody, followed by avidin-biotin peroxidase complex (ABC) (Vector laboratories, Burlingame, CA) at room temperature for 1 h. Antibody localization was detected with diaminobenzidine (DAB) as a chromogen substrate. .. The UT-B protein expression was examined under a microscope and semi-quantified as 0 ~ 4 scale: 0 = no appreciable staining or faint staining intensity in < 10% of tumor cells, 1 = faint staining in > 10% of tumor cells, 2 = readily appreciable brown staining, 3 = dark brown staining, or 4 = very strong staining.

    Article Title: The specific JNK inhibitor SP600125 targets tumour necrosis factor-? production and epithelial cell apoptosis in acute murine colitis
    Article Snippet: .. Sections were stained using a Vectastain ABC elite kit and biotinylated ant-rabbit (or anti-rat) antibody, and avidin D–fluorescein isothiocyanate used for immunofluorescence (Vector Laboratories, Burlingame, CA). ..

    Labeling:

    Article Title: Endocytosis of a Glycosylphosphatidylinositol-anchored Protein via Clathrin-coated Vesicles, Sorting by Default in Endosomes, and Exocytosis via RAB11-positive Carriers V⃞
    Article Snippet: .. In one case ( , inset), clathrin was first labeled with rabbit anti-TbCLH antibodies and PAG-6, whereas VSG was subsequently detected using biotinylated rabbit anti-VSG IgG followed by a monoclonal mouse antibiotin antibody (1:100; Vector Laboratories, Burlingame, CA) and goat anti-mouse IgG-18-nm gold conjugates (1:20; Jackson Immunoresearch Laboratories, West Grove, PA). .. After labeling, the cryosections were embedded in 1.4% methylcellulose, 0.3% uranyl acetate.

    Incubation:

    Article Title: Macrophage lipoprotein lipase modulates the development of atherosclerosis but not adiposity
    Article Snippet: .. After washing, sections were incubated with biotinylated anti-rat antibody for 1 h at room temperature, and then with avidin-biotin peroxidase complex (Vector Labs) for 30 min. Lastly, sections were developed with 3,3′-diaminobenzidine tetrahydrochloride (DAB) (Sigma), and counterstained with hematoxylin. .. The adipose tissue of mice fed a WTD for 12 weeks was fixed with 10% neutral-buffered formalin, embedded in paraffin, and sections were stained with hematoxylin and eosin.

    Article Title: Abnormalities of Thymic Stroma may Contribute to Immune Dysregulation in Murine Models of Leaky Severe Combined Immunodeficiency
    Article Snippet: .. The same procedure was applied to double immunofluorescence stainings prior to the incubation with a secondary swine anti-rabbit FITC-conjugated antibody (1:30; DAKO Cytomation, Glostrup, Denmark) for CK5 and a rabbit anti-rat biotinylated antibody (1:200; Vector Laboratories, Burlingame, CA, USA) followed by Streptavidin–Texas Red (1:100; Southern Biotechnology Associates, Birmingham, AL, USA) for CK8. ..

    Article Title: Identification of a Novel UT-B Urea Transporter in Human Urothelial Cancer
    Article Snippet: .. After a PBS wash, tissues were incubated with biotinylated anti-rabbit antibody, followed by avidin-biotin peroxidase complex (ABC) (Vector laboratories, Burlingame, CA) at room temperature for 1 h. Antibody localization was detected with diaminobenzidine (DAB) as a chromogen substrate. .. The UT-B protein expression was examined under a microscope and semi-quantified as 0 ~ 4 scale: 0 = no appreciable staining or faint staining intensity in < 10% of tumor cells, 1 = faint staining in > 10% of tumor cells, 2 = readily appreciable brown staining, 3 = dark brown staining, or 4 = very strong staining.

    Staining:

    Article Title: The specific JNK inhibitor SP600125 targets tumour necrosis factor-? production and epithelial cell apoptosis in acute murine colitis
    Article Snippet: .. Sections were stained using a Vectastain ABC elite kit and biotinylated ant-rabbit (or anti-rat) antibody, and avidin D–fluorescein isothiocyanate used for immunofluorescence (Vector Laboratories, Burlingame, CA). ..

    Immunofluorescence:

    Article Title: Abnormalities of Thymic Stroma may Contribute to Immune Dysregulation in Murine Models of Leaky Severe Combined Immunodeficiency
    Article Snippet: .. The same procedure was applied to double immunofluorescence stainings prior to the incubation with a secondary swine anti-rabbit FITC-conjugated antibody (1:30; DAKO Cytomation, Glostrup, Denmark) for CK5 and a rabbit anti-rat biotinylated antibody (1:200; Vector Laboratories, Burlingame, CA, USA) followed by Streptavidin–Texas Red (1:100; Southern Biotechnology Associates, Birmingham, AL, USA) for CK8. ..

    Article Title: The specific JNK inhibitor SP600125 targets tumour necrosis factor-? production and epithelial cell apoptosis in acute murine colitis
    Article Snippet: .. Sections were stained using a Vectastain ABC elite kit and biotinylated ant-rabbit (or anti-rat) antibody, and avidin D–fluorescein isothiocyanate used for immunofluorescence (Vector Laboratories, Burlingame, CA). ..

    Plasmid Preparation:

    Article Title: Anti-α4β1 Integrin Antibodies Attenuated Brain Inflammatory Changes in a Mouse Model of Alzheimer’s Disease
    Article Snippet: .. Elite Vectastain ABC avidin and biotin kits, biotinylated anti-rabbit, anti-mouse, and anti-rat antibodies and the Vector VIP kits were from Vector Laboratories Inc. (Burlingame, CA). .. Anti-CD68 was obtained from Serotec (Raleigh, NC).

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  • 99
    Vector Laboratories rabbit antirat secondary antibody
    Rabbit Antirat Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit antirat secondary antibody/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit antirat secondary antibody - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    97
    Vector Laboratories rabbit antirat igg biotinylated secondary antibody
    Rabbit Antirat Igg Biotinylated Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit antirat igg biotinylated secondary antibody/product/Vector Laboratories
    Average 97 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rabbit antirat igg biotinylated secondary antibody - by Bioz Stars, 2021-01
    97/100 stars
      Buy from Supplier

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