Journal: bioRxiv

Article Title: Modulation of biphasic pattern of cortical reorganization in spinal cord transected rats by external magnetic fields

doi: 10.1101/2024.04.10.588799

Figure Lengend Snippet: Areas localization procedure using NIS software for Nogo-A and BDNF. White boxes showed fields (top, field 1; bottom, field 2). M1= primary motor cortex; M2= secondary motor cortex; S1Fl= Somatosensory, forelimb area; S1HL= Somatosensory, hindlimb area.

Article Snippet: Immunofluorescence was done on brain sections to assess expression of plasticity associated proteins Nogo-A and BDNF with rabbit anti-Nogo-A (1:200; Invitrogen, USA), rabbit anti-BDNF (1:500; Invitrogen,USA), and anti-rabbit FITC labeled 2° antibody (1:400; Vectors Laboratory, USA).

Techniques: Software

Journal: bioRxiv

Article Title: Modulation of biphasic pattern of cortical reorganization in spinal cord transected rats by external magnetic fields

doi: 10.1101/2024.04.10.588799

Figure Lengend Snippet: Subcellular localization of Nogo-A (40X objective lens) of M1 (primary motor area) (1a-c); M2 (secondary motor area) (2a-c); S1FL (somatosensory forelimb area) (3a-c); S1HL (somatosensory hindlimb area) (4a-c) in (i) Control group, (ii) Sham (Day 32), (iii) SCI (Day 32), (iv) SCI+MF (Day 32).

Article Snippet: Immunofluorescence was done on brain sections to assess expression of plasticity associated proteins Nogo-A and BDNF with rabbit anti-Nogo-A (1:200; Invitrogen, USA), rabbit anti-BDNF (1:500; Invitrogen,USA), and anti-rabbit FITC labeled 2° antibody (1:400; Vectors Laboratory, USA).

Techniques:

Journal: bioRxiv

Article Title: Modulation of biphasic pattern of cortical reorganization in spinal cord transected rats by external magnetic fields

doi: 10.1101/2024.04.10.588799

Figure Lengend Snippet: Intragroup temporal comparison of Nogo-A+ cells/ field of (i) M1 (primary Motor cortex), (ii) M2 (secondary motor cortex), (iii) S1FL (somatosensory, forelimb cortex), (iv) S1HL (somatosensory, hindlimb cortex) in right cortical hemisphere (A) and left cortical hemisphere (B). Data expressed as Mean±SEM (n=3/ group). * indicates comparison between day 5 and day 12; # indicates comparison between day 12 and day 32; $ indicates comparison between day 5 and day 32. Field = 8730 μm 2 . */#/$ ≤0.05; **/##/$$ ≤0.01, ***/###/$$$ ≤0.001.

Article Snippet: Immunofluorescence was done on brain sections to assess expression of plasticity associated proteins Nogo-A and BDNF with rabbit anti-Nogo-A (1:200; Invitrogen, USA), rabbit anti-BDNF (1:500; Invitrogen,USA), and anti-rabbit FITC labeled 2° antibody (1:400; Vectors Laboratory, USA).

Techniques: Comparison

Journal: bioRxiv

Article Title: Modulation of biphasic pattern of cortical reorganization in spinal cord transected rats by external magnetic fields

doi: 10.1101/2024.04.10.588799

Figure Lengend Snippet: Intergroup comparison of Nogo-A+ cells/ field in (a) right and (b) left cortical hemisphere after MF exposure at (i) Day 5, (ii) Day 12 (iii) Day 32. M1= Primary Motor cortex (A); M2= Secondary Motor Cortex (B); S1FL= Primary somatosensory, forelimb (C); and S1HL= Primary somatosensory, hindlimb (D). Data expressed as Mean±SEM (n=3/ group). Field = 8730 μm 2 . * p ≤ 0.05; ** p ≤ 0.01; ***p ≤ 0.001

Article Snippet: Immunofluorescence was done on brain sections to assess expression of plasticity associated proteins Nogo-A and BDNF with rabbit anti-Nogo-A (1:200; Invitrogen, USA), rabbit anti-BDNF (1:500; Invitrogen,USA), and anti-rabbit FITC labeled 2° antibody (1:400; Vectors Laboratory, USA).

Techniques: Comparison

Journal: Neural Regeneration Research

Article Title: Serum response factor promotes axon regeneration following spinal cord transection injury

doi: 10.4103/1673-5374.367974

Figure Lengend Snippet: Overexpressed SRF accompanied by upregulated GAP43 after SCI. (A) Representative photomicrographs of spinal cord labeled by GAP43 (green, Alexa Fluor 488) and Nogo A (red, Alexa Fluor 594) in the caudal end of the injured spinal cord tissue. Newborn axons identified by GAP43 were increased in the LV-srf group compared with the findings in the NC group. Nogo A was displayed by red fluorescence. Scale bars: 200 μm. (B) Western blot of GAP43 protein expression. (C) Quatification of GAP43 protein expression. Data are represented as mean ± SD ( n = 3). ** P < 0.01, vs . NC group (independent samples t -test). GAP43: Growth associated protein 43; NC: negative control; SCI: spinal cord injury; SRF: serum response factor

Article Snippet: Subsequently, mouse anti-GAP43 (1:200, Santa Cruz Biotechnology, Cat# sc-17790, RRID: AB_627660) and rabbit anti-Nogo A (1:200, Invitrogen, Cat# 36-6600, RRID: AB_2533273) were added to the tissue sections and incubated at 4°C overnight.

Techniques: Labeling, Fluorescence, Western Blot, Expressing, Negative Control