rabbit anti vdac  (Cell Signaling Technology Inc)

Journal: bioRxiv

Article Title: Chronic cold exposure induces plasticity of mitochondrial calcium uptake in beige and brown fat of UCP1-deficient mice

doi: 10.64898/2026.03.16.712209

Figure Lengend Snippet: A. Swelling of BAT mitochondria from WT and Ucp1 -/- mice upon 3 weeks of cold exposure. B. Swelling of mitochondria from liver of WT mice kept at RT for comparison. C. Immunoblots of proteins implicated in ERMCs: GRP75, IP3R, VDAC, and cyclophilin D (CyD). D. Quantified protein expression. Calnexin, TOM20 and Na/K ATPase were used for normalization per ER biomass, mitochondrial biomass, and total extract, respectively. E. Representative image of PLA for IP3R–VDAC in BAT of WT and Ucp1 -/- mice exposed to 6°C for 3 weeks. F. Quantification of the contact sites from E . n = 4-6 mice. Data shown as mean ± sem. Unpaired t-test.

Article Snippet: Lysates were resolved by sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis (PAGE), transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore), and probed with antibodies against Na + /K + -ATPase (Abcam, ab76020, GR3237646-13, 1/10,000), TOMM20 (Sigma Prestige, HPA011562, 1/500), MCU (Cell Signaling Technology, 14997, 1/100), UCP1 (Abcam, ab10983, 1/1000), NCLX (Proteintech, 21430-1-AP, 1/500), TMBIM5 (Proteintech, 16296-1-AP, 1/500), TMEM65 (Invitrogen, PA5-112762, 1/500), GRP75 (Abcam, ab53098, 1/500), Calnexin (BD Transduction Laboratories, 610523, 1/1000), VDAC (Cell Signaling Technology, 4661, 1/2000), CyD (Proteintech, 18466-1-AP, 1/1000), OXPHOS (Abcam, ab110413, 1/500), Vinculin (1:10000; V9131; Sigma-Aldrich), NDUFB6 (1:5000; #6510832 Thermo Fisher Scientific), mtCOI (1:10000; #10444035; Thermo Fisher Scientific), ATP5A (1:1,000; #439800; Thermo Fisher Scientific), SDHA (1:1,000, #459200, Thermo Fisher Scientific), SDHB (1:1,000, #ab14714, Abcam), UQCRC2 (1:1,000, #14742-1-AP; Proteintech), ATPIF1 (1:1,000, #13268S; Cell Signaling Technology), TOMM20 Alexa Fluor 488 (1:1,000; #ab205486; Abcam), and cytochrome c (1:5,000, #ab110325, Abcam).

Techniques: Comparison, Western Blot, Expressing

Journal: bioRxiv

Article Title: Deep Learning-Driven Discovery of Mitochondrial Factors Modulating Influenza A Virus Infection

doi: 10.64898/2026.02.25.707858

Figure Lengend Snippet: a, Mitochondrial content assessment. Levels of PINK1, a mitophagy marker, TOM20, and VDAC, commonly used mitochondrial markers, were assessed in infected and mock-infected HeLa cells. Whole-cell lysates were analyzed with anti-PINK1, anti-TOM20, and anti-VDAC antibodies. The values were normalized with GAPDH or β-tubulin levels. b, Abundance levels of nine mitochondrial proteins were analyzed in whole-cell lysates obtained from infected and mock-infected HeLa cells. Specific antibodies were used to assess each protein. The values were normalized with β-tubulin or GAPDH levels. c, Abundance levels of CH60, OXA1L, and TIM44 proteins were assessed in biotin-phenol labeled and enriched samples from infected and mock-infected HeLa cells. The samples were prepared simultaneously, and equal amounts were loaded onto the gel. Specific antibodies were used to assess each protein. On all graphs in this figure, the bars represent the normalized means ± s.d. Statistical significance was calculated using a parametric unpaired t-test, *p < 0.05.

Article Snippet: Primary antibodies used: anti-GAPDH (1:2000; rabbit, Cell Signaling Technology, 2118) anti-beta Tubulin (1:2000; rabbit, Abcam, ab15568) anti-beta Tubulin (1:2000; mouse, Thermo Fisher Scientific, MA5-16308) anti-V5 tag (1:1000; mouse, Thermo Fisher Scientific, MA5-15253) anti-CH60 (1:6000; rabbit, Proteintech, 15282-1-AP) anti-ETHE1 (1:1000; rabbit, Proteintech, 27786-1-AP) anti-GRP75 (1:500; mouse, Thermo Fisher Scientific, MA3-028) anti-LETM1 (1:1000; rabbit, Proteintech, 16024-1-AP) anti-LONM (1:1000; rabbit, Proteintech, 15440-1-AP) anti-OXA1L (1:2000; rabbit, Proteintech, 21055-1-AP) anti-MPPB (1:1000; rabbit, Proteintech, 16064-1-AP) anti-SQOR (1:1000; rabbit, Proteintech, 17256-1-AP) anti-TIM44 (1:1000; rabbit, Abcam, ab244466) anti-TOMM20 (1:500; mouse, Abcam, ab56783) anti-VDAC (1:1000; rabbit, Cell Signaling Technology, 4661).

Techniques: Marker, Infection, Labeling