Journal: Scientific Reports

Article Title: Antifibrotic effect of disulfiram on bleomycin-induced lung fibrosis in mice and its impact on macrophage infiltration

doi: 10.1038/s41598-024-71770-z

Figure Lengend Snippet: Effect of DSF on S100A4 + macrophages in the lung tissues of BLM-induced lung fibrosis in mice. ( A – C ) Representative immunostaining images for S100A4 in the lung tissue of untreated ( A ), DSF non-treated ( B ) and DSF-treated ( C ) mice on day 28. Higher-magnification images in the lower left correspond to the areas indicated by dashed squares. Scale bars = 200 μm. ( D ) Representative bright-field and double-fluorescence staining images in the lung tissues of BLM-treated mice on day 28. DAPI (4′,6-diamidino-2-phenylindole) is shown in blue, CD68 in green, and S100A4 in red. The bright-field image was generated from fluorescent images using inForm software. Higher-magnification images correspond to the area indicated with dashed squares. Arrowheads indicate S100A4 + CD68 + macrophages. Scale bars = 25 μm. ( E ) Number of S100A4 + cells in the lung tissue on day 28 counted in immunostaining images ( A – C ). n = 5 per group. * P < 0.05, ** P < 0.01 (Tukey’s test). ns, not significant.

Article Snippet: We used the following primary antibodies: anti-human α-smooth muscle actin (α-SMA) mouse monoclonal antibody (1:250 dilution, Dako, Glostrup, Denmark), anti-human CD34 mouse monoclonal antibody (1:100 dilution, Nichirei Bioscience, Tokyo, Japan), anti-human CD68 mouse monoclonal antibody (1:200 dilution, Dako, Glostrup, Denmark), anti-human/mouse S100A4 rabbit monoclonal antibody (1:100 dilution, Abcam, Cambridge, UK), and anti-human FROUNT goat polyclonal antibody (1:150 dilution, Everest biotech, Oxfordshire, UK), followed by peroxidase-labelled secondary antibodies, and incubated with Opal fluorophore Opal 690, Opal 540, Opal 570, Opal 620, Opal 520, respectively.

Techniques: Immunostaining, Fluorescence, Staining, Generated, Software

Journal: Scientific Reports

Article Title: Antifibrotic effect of disulfiram on bleomycin-induced lung fibrosis in mice and its impact on macrophage infiltration

doi: 10.1038/s41598-024-71770-z

Figure Lengend Snippet: Analysis of multi-fluorescent staining of lung tissues of IPF. ( A , B ) Representative immunofluorescent staining images in normal lung ( A ) and in fibrotic areas of the lungs of IPF patients ( B ). DAPI is shown in pink, α-SMA in brown, CD34 in light blue, CD68 in green, S100A4 in red, and FROUNT in blue. Higher-magnification images on the right correspond to the area indicated with dashed squares. White arrows indicate CD68 + macrophages in normal lung. White arrowheads indicate S100A4 + CD68 + macrophages and yellow arrowheads indicate S100A4 - CD68 + macrophages in fibrotic areas of the lungs of IPF patients. Scale bars = 25 μm. ( C ) S100A4 signals of each CD68 + macrophage in normal lung tissues and in fibrotic areas of the lungs of IPF patients. ( D ) FROUNT signals in S100A4 - CD68 + macrophages and S100A4 + CD68 + macrophages in fibrotic areas of the lungs of IPF patients. n = 5 or 6 per group. ** P < 0.01 (unpaired t-test).

Article Snippet: We used the following primary antibodies: anti-human α-smooth muscle actin (α-SMA) mouse monoclonal antibody (1:250 dilution, Dako, Glostrup, Denmark), anti-human CD34 mouse monoclonal antibody (1:100 dilution, Nichirei Bioscience, Tokyo, Japan), anti-human CD68 mouse monoclonal antibody (1:200 dilution, Dako, Glostrup, Denmark), anti-human/mouse S100A4 rabbit monoclonal antibody (1:100 dilution, Abcam, Cambridge, UK), and anti-human FROUNT goat polyclonal antibody (1:150 dilution, Everest biotech, Oxfordshire, UK), followed by peroxidase-labelled secondary antibodies, and incubated with Opal fluorophore Opal 690, Opal 540, Opal 570, Opal 620, Opal 520, respectively.

Techniques: Staining

Journal: Scientific Reports

Article Title: Antifibrotic effect of disulfiram on bleomycin-induced lung fibrosis in mice and its impact on macrophage infiltration

doi: 10.1038/s41598-024-71770-z

Figure Lengend Snippet: Effect of DSF on S100A4 + macrophages in the lung tissues of BLM-induced lung fibrosis in mice. ( A – C ) Representative immunostaining images for S100A4 in the lung tissue of untreated ( A ), DSF non-treated ( B ) and DSF-treated ( C ) mice on day 28. Higher-magnification images in the lower left correspond to the areas indicated by dashed squares. Scale bars = 200 μm. ( D ) Representative bright-field and double-fluorescence staining images in the lung tissues of BLM-treated mice on day 28. DAPI (4′,6-diamidino-2-phenylindole) is shown in blue, CD68 in green, and S100A4 in red. The bright-field image was generated from fluorescent images using inForm software. Higher-magnification images correspond to the area indicated with dashed squares. Arrowheads indicate S100A4 + CD68 + macrophages. Scale bars = 25 μm. ( E ) Number of S100A4 + cells in the lung tissue on day 28 counted in immunostaining images ( A – C ). n = 5 per group. * P < 0.05, ** P < 0.01 (Tukey’s test). ns, not significant.

Article Snippet: We used the following primary antibodies: anti-human α-smooth muscle actin (α-SMA) mouse monoclonal antibody (1:250 dilution, Dako, Glostrup, Denmark), anti-human CD34 mouse monoclonal antibody (1:100 dilution, Nichirei Bioscience, Tokyo, Japan), anti-human CD68 mouse monoclonal antibody (1:200 dilution, Dako, Glostrup, Denmark), anti-human/mouse S100A4 rabbit monoclonal antibody (1:100 dilution, Abcam, Cambridge, UK), and anti-human FROUNT goat polyclonal antibody (1:150 dilution, Everest biotech, Oxfordshire, UK), followed by peroxidase-labelled secondary antibodies, and incubated with Opal fluorophore Opal 690, Opal 540, Opal 570, Opal 620, Opal 520, respectively.

Techniques: Immunostaining, Fluorescence, Staining, Generated, Software

Journal: Scientific Reports

Article Title: Antifibrotic effect of disulfiram on bleomycin-induced lung fibrosis in mice and its impact on macrophage infiltration

doi: 10.1038/s41598-024-71770-z

Figure Lengend Snippet: Analysis of multi-fluorescent staining of lung tissues of IPF. ( A , B ) Representative immunofluorescent staining images in normal lung ( A ) and in fibrotic areas of the lungs of IPF patients ( B ). DAPI is shown in pink, α-SMA in brown, CD34 in light blue, CD68 in green, S100A4 in red, and FROUNT in blue. Higher-magnification images on the right correspond to the area indicated with dashed squares. White arrows indicate CD68 + macrophages in normal lung. White arrowheads indicate S100A4 + CD68 + macrophages and yellow arrowheads indicate S100A4 - CD68 + macrophages in fibrotic areas of the lungs of IPF patients. Scale bars = 25 μm. ( C ) S100A4 signals of each CD68 + macrophage in normal lung tissues and in fibrotic areas of the lungs of IPF patients. ( D ) FROUNT signals in S100A4 - CD68 + macrophages and S100A4 + CD68 + macrophages in fibrotic areas of the lungs of IPF patients. n = 5 or 6 per group. ** P < 0.01 (unpaired t-test).

Article Snippet: We used the following primary antibodies: anti-human α-smooth muscle actin (α-SMA) mouse monoclonal antibody (1:250 dilution, Dako, Glostrup, Denmark), anti-human CD34 mouse monoclonal antibody (1:100 dilution, Nichirei Bioscience, Tokyo, Japan), anti-human CD68 mouse monoclonal antibody (1:200 dilution, Dako, Glostrup, Denmark), anti-human/mouse S100A4 rabbit monoclonal antibody (1:100 dilution, Abcam, Cambridge, UK), and anti-human FROUNT goat polyclonal antibody (1:150 dilution, Everest biotech, Oxfordshire, UK), followed by peroxidase-labelled secondary antibodies, and incubated with Opal fluorophore Opal 690, Opal 540, Opal 570, Opal 620, Opal 520, respectively.

Techniques: Staining

Journal: Scientific Reports

Article Title: Antifibrotic effect of disulfiram on bleomycin-induced lung fibrosis in mice and its impact on macrophage infiltration

doi: 10.1038/s41598-024-71770-z

Figure Lengend Snippet: Effect of DSF on S100A4 + macrophages in the lung tissues of BLM-induced lung fibrosis in mice. ( A – C ) Representative immunostaining images for S100A4 in the lung tissue of untreated ( A ), DSF non-treated ( B ) and DSF-treated ( C ) mice on day 28. Higher-magnification images in the lower left correspond to the areas indicated by dashed squares. Scale bars = 200 μm. ( D ) Representative bright-field and double-fluorescence staining images in the lung tissues of BLM-treated mice on day 28. DAPI (4′,6-diamidino-2-phenylindole) is shown in blue, CD68 in green, and S100A4 in red. The bright-field image was generated from fluorescent images using inForm software. Higher-magnification images correspond to the area indicated with dashed squares. Arrowheads indicate S100A4 + CD68 + macrophages. Scale bars = 25 μm. ( E ) Number of S100A4 + cells in the lung tissue on day 28 counted in immunostaining images ( A – C ). n = 5 per group. * P < 0.05, ** P < 0.01 (Tukey’s test). ns, not significant.

Article Snippet: We used the following primary antibodies: anti-human α-smooth muscle actin (α-SMA) mouse monoclonal antibody (1:250 dilution, Dako, Glostrup, Denmark), anti-human CD34 mouse monoclonal antibody (1:100 dilution, Nichirei Bioscience, Tokyo, Japan), anti-human CD68 mouse monoclonal antibody (1:200 dilution, Dako, Glostrup, Denmark), anti-human/mouse S100A4 rabbit monoclonal antibody (1:100 dilution, Abcam, Cambridge, UK), and anti-human FROUNT goat polyclonal antibody (1:150 dilution, Everest biotech, Oxfordshire, UK), followed by peroxidase-labelled secondary antibodies, and incubated with Opal fluorophore Opal 690, Opal 540, Opal 570, Opal 620, Opal 520, respectively.

Techniques: Immunostaining, Fluorescence, Staining, Generated, Software

Journal: Scientific Reports

Article Title: Antifibrotic effect of disulfiram on bleomycin-induced lung fibrosis in mice and its impact on macrophage infiltration

doi: 10.1038/s41598-024-71770-z

Figure Lengend Snippet: Analysis of multi-fluorescent staining of lung tissues of IPF. ( A , B ) Representative immunofluorescent staining images in normal lung ( A ) and in fibrotic areas of the lungs of IPF patients ( B ). DAPI is shown in pink, α-SMA in brown, CD34 in light blue, CD68 in green, S100A4 in red, and FROUNT in blue. Higher-magnification images on the right correspond to the area indicated with dashed squares. White arrows indicate CD68 + macrophages in normal lung. White arrowheads indicate S100A4 + CD68 + macrophages and yellow arrowheads indicate S100A4 - CD68 + macrophages in fibrotic areas of the lungs of IPF patients. Scale bars = 25 μm. ( C ) S100A4 signals of each CD68 + macrophage in normal lung tissues and in fibrotic areas of the lungs of IPF patients. ( D ) FROUNT signals in S100A4 - CD68 + macrophages and S100A4 + CD68 + macrophages in fibrotic areas of the lungs of IPF patients. n = 5 or 6 per group. ** P < 0.01 (unpaired t-test).

Article Snippet: We used the following primary antibodies: anti-human α-smooth muscle actin (α-SMA) mouse monoclonal antibody (1:250 dilution, Dako, Glostrup, Denmark), anti-human CD34 mouse monoclonal antibody (1:100 dilution, Nichirei Bioscience, Tokyo, Japan), anti-human CD68 mouse monoclonal antibody (1:200 dilution, Dako, Glostrup, Denmark), anti-human/mouse S100A4 rabbit monoclonal antibody (1:100 dilution, Abcam, Cambridge, UK), and anti-human FROUNT goat polyclonal antibody (1:150 dilution, Everest biotech, Oxfordshire, UK), followed by peroxidase-labelled secondary antibodies, and incubated with Opal fluorophore Opal 690, Opal 540, Opal 570, Opal 620, Opal 520, respectively.

Techniques: Staining

Journal: Scientific Reports

Article Title: Antifibrotic effect of disulfiram on bleomycin-induced lung fibrosis in mice and its impact on macrophage infiltration

doi: 10.1038/s41598-024-71770-z

Figure Lengend Snippet: Effect of DSF on S100A4 + macrophages in the lung tissues of BLM-induced lung fibrosis in mice. ( A – C ) Representative immunostaining images for S100A4 in the lung tissue of untreated ( A ), DSF non-treated ( B ) and DSF-treated ( C ) mice on day 28. Higher-magnification images in the lower left correspond to the areas indicated by dashed squares. Scale bars = 200 μm. ( D ) Representative bright-field and double-fluorescence staining images in the lung tissues of BLM-treated mice on day 28. DAPI (4′,6-diamidino-2-phenylindole) is shown in blue, CD68 in green, and S100A4 in red. The bright-field image was generated from fluorescent images using inForm software. Higher-magnification images correspond to the area indicated with dashed squares. Arrowheads indicate S100A4 + CD68 + macrophages. Scale bars = 25 μm. ( E ) Number of S100A4 + cells in the lung tissue on day 28 counted in immunostaining images ( A – C ). n = 5 per group. * P < 0.05, ** P < 0.01 (Tukey’s test). ns, not significant.

Article Snippet: We used the following primary antibodies: anti-human α-smooth muscle actin (α-SMA) mouse monoclonal antibody (1:250 dilution, Dako, Glostrup, Denmark), anti-human CD34 mouse monoclonal antibody (1:100 dilution, Nichirei Bioscience, Tokyo, Japan), anti-human CD68 mouse monoclonal antibody (1:200 dilution, Dako, Glostrup, Denmark), anti-human/mouse S100A4 rabbit monoclonal antibody (1:100 dilution, Abcam, Cambridge, UK), and anti-human FROUNT goat polyclonal antibody (1:150 dilution, Everest biotech, Oxfordshire, UK), followed by peroxidase-labelled secondary antibodies, and incubated with Opal fluorophore Opal 690, Opal 540, Opal 570, Opal 620, Opal 520, respectively.

Techniques: Immunostaining, Fluorescence, Staining, Generated, Software

Journal: Scientific Reports

Article Title: Antifibrotic effect of disulfiram on bleomycin-induced lung fibrosis in mice and its impact on macrophage infiltration

doi: 10.1038/s41598-024-71770-z

Figure Lengend Snippet: Analysis of multi-fluorescent staining of lung tissues of IPF. ( A , B ) Representative immunofluorescent staining images in normal lung ( A ) and in fibrotic areas of the lungs of IPF patients ( B ). DAPI is shown in pink, α-SMA in brown, CD34 in light blue, CD68 in green, S100A4 in red, and FROUNT in blue. Higher-magnification images on the right correspond to the area indicated with dashed squares. White arrows indicate CD68 + macrophages in normal lung. White arrowheads indicate S100A4 + CD68 + macrophages and yellow arrowheads indicate S100A4 - CD68 + macrophages in fibrotic areas of the lungs of IPF patients. Scale bars = 25 μm. ( C ) S100A4 signals of each CD68 + macrophage in normal lung tissues and in fibrotic areas of the lungs of IPF patients. ( D ) FROUNT signals in S100A4 - CD68 + macrophages and S100A4 + CD68 + macrophages in fibrotic areas of the lungs of IPF patients. n = 5 or 6 per group. ** P < 0.01 (unpaired t-test).

Article Snippet: We used the following primary antibodies: anti-human α-smooth muscle actin (α-SMA) mouse monoclonal antibody (1:250 dilution, Dako, Glostrup, Denmark), anti-human CD34 mouse monoclonal antibody (1:100 dilution, Nichirei Bioscience, Tokyo, Japan), anti-human CD68 mouse monoclonal antibody (1:200 dilution, Dako, Glostrup, Denmark), anti-human/mouse S100A4 rabbit monoclonal antibody (1:100 dilution, Abcam, Cambridge, UK), and anti-human FROUNT goat polyclonal antibody (1:150 dilution, Everest biotech, Oxfordshire, UK), followed by peroxidase-labelled secondary antibodies, and incubated with Opal fluorophore Opal 690, Opal 540, Opal 570, Opal 620, Opal 520, respectively.

Techniques: Staining

Journal: bioRxiv

Article Title: Mapping herpesvirus-driven impacts on the cellular milieu and transcriptional profile of Kaposi sarcoma in patient-derived mouse models

doi: 10.1101/2024.09.27.615429

Figure Lengend Snippet: (A) Two KS PDX explants were embedded in extracellular matrix in the presence of ROCK inhibitor; cells sprouted and expanded over 45 d to generate PDX- derived cells (KSX-476 and KSX-488) that were maintained for 15 passages. (B) Cell lysates of KSXs, HFF and endothelial cells were analyzed for markers of endothelial cells (CD31 & VEGFR3), fibroblasts (PDGFR-α & PDGRF-β) and TAFs (a-SMA, FAB, and S100A4) by immunoblot. (C) KSX-476 and -488 were inoculated with KSHV-BAC16 at MOI 1.0, and KSHV infection was monitored by the GFP+ cells by microscopy. (D) KSX-476 and -488 were infected with KSHV-BAC16 at MOI 1, and the level of the indicated KSHV transcripts was determined relative to GAPDH over 8 d. (E) The fold change in GAPDH-normalized expression of CXCL12 in KSHV- infected KSX-476 and -488 was determined relative to day 0 prior to infection. (F) The conditioned media collected from mock and KSHV-infected KSXs at 4 dpi was subjected to ELISA to measure the extracellular level of CXCL12 protein. (G) Spatial distribution of CXCL12 (upper) and CXCR4 (lower) normalized expression levels in fibroblast and KS signature clusters in the indicated samples.

Article Snippet: Total cell extracts in Tris-Glycine SDS Sample Buffer (cat# LC2676, Thermo Fisher) were electrophoresed in 4–20% SDS-polyacrylamide gels (cat# XP04200BOX, Thermo Fisher), transferred to nitrocellulose membranes, and blotted with indicated antibodies including mouse anti-CD31 (cat# 3528; Cell Signaling Technology), rabbit anti-PDGF Receptor α (3174; Cell Signaling Technology), rabbit anti-PDGF Receptor β (cat# 3169; Cell Signaling Technology), rabbit anti-α-Smooth Muscle Actin (cat# 19245; Cell Signaling Technology), rabbit anti-VEGF Receptor 3 (cat# 2638; Cell Signaling Technology), rabbit anti-FAP (cat# 66562, Cell Signaling Technology), rabbit anti-S100A4 (cat# 13018, Cell Signaling Technology) and rabbit anti- GAPDH (cat# 2118; Cell Signaling Technology).

Techniques: Derivative Assay, Western Blot, Infection, Microscopy, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Cancer Cell

Article Title: Cancer-associated fibroblast phenotypes are associated with patient outcome in non-small cell lung cancer

doi: 10.1016/j.ccell.2023.12.021

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti-FSP1 / S100A4; Clone (EPR2761(2)); Lot(GR317174-3) , Abcam , Cat#136784; RRID: AB_10807552.

Techniques: Recombinant, Labeling, Software

Journal: iScience

Article Title: S100A4/NF-κB axis mediates the anticancer effect of epigallocatechin-3-gallate in platinum-resistant ovarian cancer

doi: 10.1016/j.isci.2024.108885

Figure Lengend Snippet: EGCG modulated S100A4, NF-κB p65, and p53 expression in DDP-resistant OC cells (A) Bioinformatics prediction of S100A4 expression in OC, analyzed by GEPIA (including 426 tumor samples and 88 paired non-cancerous (normal) ovarian tissues). (B and C) The mRNA and protein expression of S100A4 were detected by qRT-PCR and western blot in Human normal ovarian epithelial cells and OC cells. (D) Western blot analysis showed the S100A4, NF-κB p65, and p53 expression with or without EGCG induced in DDP-resistant OC. (E) Cells transfected with S100A4 plasmid restored the S100A4 expression which inhibited by EGCG, protein expression was analyzed by western blot. Data were represented as mean ± SD, n = 3, differences among groups were assessed by ANOVA. ∗∗p < 0.01, ∗∗∗p < 0.001, ns: no significantly.

Article Snippet: Rabbit anti-S100A4 , Servicebio , GB11397.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Transfection, Plasmid Preparation

Journal: iScience

Article Title: S100A4/NF-κB axis mediates the anticancer effect of epigallocatechin-3-gallate in platinum-resistant ovarian cancer

doi: 10.1016/j.isci.2024.108885

Figure Lengend Snippet: S100A4 overexpression rescinds the anticancer effect of EGCG in DDP-resistant OC (A) The cell viability of SKOV3/DDP and A2780/DDP cells were monitored by CCK8 assay for 24, 48, and 72 h. Four treatment groups included Blank, EGCG, EGCG + vector, and EGCG + S100A4. (B) Cells transfected with S100A4 plasmid or vector plasmid were treated with EGCG for 48 h. Colonies formation assay were terminated after two weeks. (C) Cell apoptosis was assessed by TUNEL test, scale bar, 50 μm. (D) The migration of each group was detected by wound healing, scale bar, 500 μm. (E) The number of cells that passed through Matrigel-coated membranes was accessed by Matrigel invasion assay, scale bar, 100 μm. (F) NF-κB p65 and p53 expression were detected by western blot with vector or S100A4 overexpression. ANOVA was used for differences among groups assessment, data were represented as mean ± SD (n = 3), ∗∗p < 0.01, ∗∗∗p < 0.001, ns: no significantly.

Article Snippet: Rabbit anti-S100A4 , Servicebio , GB11397.

Techniques: Over Expression, CCK-8 Assay, Plasmid Preparation, Transfection, Tube Formation Assay, TUNEL Assay, Migration, Invasion Assay, Expressing, Western Blot

Journal: iScience

Article Title: S100A4/NF-κB axis mediates the anticancer effect of epigallocatechin-3-gallate in platinum-resistant ovarian cancer

doi: 10.1016/j.isci.2024.108885

Figure Lengend Snippet: NF-κB promotes OC progression in DDP-resistant cells (A) The cell viability of SKOV3/DDP and A2780/DDP cells were monitored by CCK8 assay for 24, 48, and 72 h. Four treatment groups included si-NC, si-S100A4, si-S100A4 + vector, and si-S100A4 + NF-κB. (B) Colonies formation assay was terminated after two weeks. (C) Cell apoptosis was assessed by TUNEL test, scale bar, 50 μm. (D) The migration of each group was detected by wound healing, scale bar, 500 μm. (E) The number of cells that passed through Matrigel-coated membranes was accessed by Matrigel invasion assay, scale bar, 100 μm. (F) NF-κB p65 and p53 expression were detected by western blot with vector or S100A4 overexpression. ANOVA was used for differences among groups assessment, data were represented as mean ± SD (n = 3), ∗∗p < 0.01, ∗∗∗p < 0.001, ns: no significantly.

Article Snippet: Rabbit anti-S100A4 , Servicebio , GB11397.

Techniques: CCK-8 Assay, Plasmid Preparation, Tube Formation Assay, TUNEL Assay, Migration, Invasion Assay, Expressing, Western Blot, Over Expression

Journal: iScience

Article Title: S100A4/NF-κB axis mediates the anticancer effect of epigallocatechin-3-gallate in platinum-resistant ovarian cancer

doi: 10.1016/j.isci.2024.108885

Figure Lengend Snippet: EGCG inhibited DDP resistance cells malignancy in xenografted A2780 cells (A) Ten 5-week-old BALB/c nude mice were injected with 2 × 10 5 A2780 cells each. Two treatment groups included Blank and EGCG. Solid tumors were peeled from mouse subcutaneous tissue. (B) Tumor size changed in a time-dependent manner, and tumor weight changes in two groups after sacrificed. In B of tumor volume, ANOVA was used for differences among groups assessment, in panel B of tumor weight, Student’s t test was used. (C) IHC staining of S100A4 and KI-67 in tumor tissues (40×), scale bar, 20 μm. (D) Western blotting was used to assess S100A4, NF-κB p65, and p53 levels in tumor tissues, with GAPDH as an internal control. In C and D, differences among groups were assessed by ANOVA, ∗∗p < 0.01, ∗∗∗p < 0.001. Data are represented as mean ± SD (n = 5).

Article Snippet: Rabbit anti-S100A4 , Servicebio , GB11397.

Techniques: Injection, Immunohistochemistry, Western Blot

Journal: iScience

Article Title: S100A4/NF-κB axis mediates the anticancer effect of epigallocatechin-3-gallate in platinum-resistant ovarian cancer

doi: 10.1016/j.isci.2024.108885

Figure Lengend Snippet:

Article Snippet: Rabbit anti-S100A4 , Servicebio , GB11397.

Techniques: Labeling, Recombinant, Western Blot, Marker, SYBR Green Assay, TUNEL Assay, Luciferase, Activity Assay, Software