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Agilent technologies rabbit anti rat igg
Immunolocalization of <t>LipY(ΔPE)</t> at the surface of M. bovis BCG. Immunolabelings were performed on whole M. bovis BCG carrying pMV261:: lipY (Δ PE ), deposited on EM Formvar-coated nickel grids prior to labeling (A and B) or onto prefixed bacteria labeled in suspension prior to processing for conventional EM (C to F). In both cases, bacteria were sequentially exposed to rat anti-LipY(ΔPE) antibodies, rabbit anti-rat <t>IgG,</t> and PAO. (A) Whole bacteria on grids exposed to specific antibody, followed by rabbit anti-rat IgG and PAO: the gold particles (300 to 400 per bacterium) are distributed throughout the bacterial surface (arrows). (B) Whole bacteria on grids exposed to rabbit anti-rat IgG and PAO only as a control: bacteria display at most 40 gold particles on their surface (arrow). (C to E) Thin sections of bacteria exposed to specific antibody, followed by rabbit anti-rat IgG and PAO. (C) The outermost layer of the cell wall is labeled (arrow). This is particularly obvious in the enlarged view (D), where the peptidoglycan layer (PG), the thin electron-translucent layer (ETL), and the outermost fibrillar layer (OL) are clearly visible. (E) When the OL has been shed, bacteria are not labeled. (F) Thin sections of bacteria exposed to preimmune rat serum, followed by rabbit anti-rat IgG and PAO: bacteria are not labeled even when the OL is present. Bars in panels A and B = 0.5 μm; Bars in panels C, E, and F = 0.25 μm; Bar in panel D = 0.1 μm.
Rabbit Anti Rat Igg, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti rat igg/product/Agilent technologies
Average 92 stars, based on 30 article reviews
Price from $9.99 to $1999.99
rabbit anti rat igg - by Bioz Stars, 2020-09
92/100 stars

Images

1) Product Images from "Functional Role of the PE Domain and Immunogenicity of the Mycobacterium tuberculosis Triacylglycerol Hydrolase LipY ▿"

Article Title: Functional Role of the PE Domain and Immunogenicity of the Mycobacterium tuberculosis Triacylglycerol Hydrolase LipY ▿

Journal: Infection and Immunity

doi: 10.1128/IAI.00410-07

Immunolocalization of LipY(ΔPE) at the surface of M. bovis BCG. Immunolabelings were performed on whole M. bovis BCG carrying pMV261:: lipY (Δ PE ), deposited on EM Formvar-coated nickel grids prior to labeling (A and B) or onto prefixed bacteria labeled in suspension prior to processing for conventional EM (C to F). In both cases, bacteria were sequentially exposed to rat anti-LipY(ΔPE) antibodies, rabbit anti-rat IgG, and PAO. (A) Whole bacteria on grids exposed to specific antibody, followed by rabbit anti-rat IgG and PAO: the gold particles (300 to 400 per bacterium) are distributed throughout the bacterial surface (arrows). (B) Whole bacteria on grids exposed to rabbit anti-rat IgG and PAO only as a control: bacteria display at most 40 gold particles on their surface (arrow). (C to E) Thin sections of bacteria exposed to specific antibody, followed by rabbit anti-rat IgG and PAO. (C) The outermost layer of the cell wall is labeled (arrow). This is particularly obvious in the enlarged view (D), where the peptidoglycan layer (PG), the thin electron-translucent layer (ETL), and the outermost fibrillar layer (OL) are clearly visible. (E) When the OL has been shed, bacteria are not labeled. (F) Thin sections of bacteria exposed to preimmune rat serum, followed by rabbit anti-rat IgG and PAO: bacteria are not labeled even when the OL is present. Bars in panels A and B = 0.5 μm; Bars in panels C, E, and F = 0.25 μm; Bar in panel D = 0.1 μm.
Figure Legend Snippet: Immunolocalization of LipY(ΔPE) at the surface of M. bovis BCG. Immunolabelings were performed on whole M. bovis BCG carrying pMV261:: lipY (Δ PE ), deposited on EM Formvar-coated nickel grids prior to labeling (A and B) or onto prefixed bacteria labeled in suspension prior to processing for conventional EM (C to F). In both cases, bacteria were sequentially exposed to rat anti-LipY(ΔPE) antibodies, rabbit anti-rat IgG, and PAO. (A) Whole bacteria on grids exposed to specific antibody, followed by rabbit anti-rat IgG and PAO: the gold particles (300 to 400 per bacterium) are distributed throughout the bacterial surface (arrows). (B) Whole bacteria on grids exposed to rabbit anti-rat IgG and PAO only as a control: bacteria display at most 40 gold particles on their surface (arrow). (C to E) Thin sections of bacteria exposed to specific antibody, followed by rabbit anti-rat IgG and PAO. (C) The outermost layer of the cell wall is labeled (arrow). This is particularly obvious in the enlarged view (D), where the peptidoglycan layer (PG), the thin electron-translucent layer (ETL), and the outermost fibrillar layer (OL) are clearly visible. (E) When the OL has been shed, bacteria are not labeled. (F) Thin sections of bacteria exposed to preimmune rat serum, followed by rabbit anti-rat IgG and PAO: bacteria are not labeled even when the OL is present. Bars in panels A and B = 0.5 μm; Bars in panels C, E, and F = 0.25 μm; Bar in panel D = 0.1 μm.

Techniques Used: Labeling

Localization of LipY and LipY(ΔPE) in M. bovis BCG. (A) Subcellular localization of LipY and LipY(ΔPE) in M. bovis BCG strains. Cultures were lysed and fractionated to separate the cytoplasm (Cy) from the cell wall (CW). Equal amounts of proteins (10 μg) of each fraction were subjected to SDS-PAGE, electroblotted onto a nitrocellulose membrane, and probed with either rat anti-LipY(ΔPE) antiserum (top), monoclonal anti-KatG antibodies (middle), or rabbit anti-OmpATb antiserum (bottom). (B) EM immunolocalization of LipY. Thin sections of cryosubstituted M. bovis BCG LipY(ΔPE) were sequentially incubated on drops of (i) PBS containing 5% BSA and 0.1% Tween 20 for 15 min, (ii) rat anti-LipY(ΔPE) antibody for 2 h, (iii) rabbit anti-rat IgG for 1 h, and (iv) PAO for 30 min. Intracytoplasmic labeling is indicated by arrows and surface labeling by arrowheads. Bar = 0.5 μm.
Figure Legend Snippet: Localization of LipY and LipY(ΔPE) in M. bovis BCG. (A) Subcellular localization of LipY and LipY(ΔPE) in M. bovis BCG strains. Cultures were lysed and fractionated to separate the cytoplasm (Cy) from the cell wall (CW). Equal amounts of proteins (10 μg) of each fraction were subjected to SDS-PAGE, electroblotted onto a nitrocellulose membrane, and probed with either rat anti-LipY(ΔPE) antiserum (top), monoclonal anti-KatG antibodies (middle), or rabbit anti-OmpATb antiserum (bottom). (B) EM immunolocalization of LipY. Thin sections of cryosubstituted M. bovis BCG LipY(ΔPE) were sequentially incubated on drops of (i) PBS containing 5% BSA and 0.1% Tween 20 for 15 min, (ii) rat anti-LipY(ΔPE) antibody for 2 h, (iii) rabbit anti-rat IgG for 1 h, and (iv) PAO for 30 min. Intracytoplasmic labeling is indicated by arrows and surface labeling by arrowheads. Bar = 0.5 μm.

Techniques Used: SDS Page, Incubation, Labeling

Specific anti-LipY humoral responses in M. tuberculosis -infected patients as opposed to healthy controls. (A) Purification of the recombinant LipY and LipY(ΔPE) proteins expressed in M. smegmatis carrying either pSD26:: lipY or pSD26:: lipY (Δ PE ). Proteins were extracted from M. smegmatis cell wall preparations, excised from preparative polyacrylamide gels, and then electroeluted to obtain pure proteins. (B) ELISA reactivities of IgG and IgM anti-LipY and anti-LipY(ΔPE) antibodies were assayed in sera of either M. tuberculosis -infected group 1 adult patients or healthy controls (HC) ( n = 44 for healthy controls; n = 69 for patients). (C and D) ELISA reactivities of anti-LipY and anti-LipY(ΔPE) antibodies in two different categories of infected children. (C) IgG and IgM reactivities of sera from recently M. tuberculosis -infected children and from healthy controls ( n = 12 for healthy controls; n = 30 for patients). (D) IgG reactivities for patients with extrapulmonary TB ( n = 12 for healthy controls; n = 27 for patients).
Figure Legend Snippet: Specific anti-LipY humoral responses in M. tuberculosis -infected patients as opposed to healthy controls. (A) Purification of the recombinant LipY and LipY(ΔPE) proteins expressed in M. smegmatis carrying either pSD26:: lipY or pSD26:: lipY (Δ PE ). Proteins were extracted from M. smegmatis cell wall preparations, excised from preparative polyacrylamide gels, and then electroeluted to obtain pure proteins. (B) ELISA reactivities of IgG and IgM anti-LipY and anti-LipY(ΔPE) antibodies were assayed in sera of either M. tuberculosis -infected group 1 adult patients or healthy controls (HC) ( n = 44 for healthy controls; n = 69 for patients). (C and D) ELISA reactivities of anti-LipY and anti-LipY(ΔPE) antibodies in two different categories of infected children. (C) IgG and IgM reactivities of sera from recently M. tuberculosis -infected children and from healthy controls ( n = 12 for healthy controls; n = 30 for patients). (D) IgG reactivities for patients with extrapulmonary TB ( n = 12 for healthy controls; n = 27 for patients).

Techniques Used: Infection, Purification, Recombinant, Enzyme-linked Immunosorbent Assay

2) Product Images from "Inducible nitric oxide synthase is critical for immune-mediated liver injury in mice"

Article Title: Inducible nitric oxide synthase is critical for immune-mediated liver injury in mice

Journal: Journal of Clinical Investigation

doi:

Detection of iNOS in livers of Con A–treated mice by immunofluorescence staining. ( a ) Con A–induced iNOS expression in livers of BALB/c mice was detected by immunofluorescence staining of liver cryostat sections (rabbit anti-mouse iNOS antiserum, secondary goat anti-rabbit IgG tagged with Cy3; red fluorescence). ( b ) Costaining of iNOS (rabbit anti-mouse iNOS antiserum, secondary swine anti-rabbit IgG tagged with FITC; green fluorescence) and Kupffer cells (BM8, secondary goat anti-rat IgG tagged with Texas red; red fluorescence) 4 hours after Con A or GalN/LPS treatment. All sections were examined by confocal laser-scanning microscopy. Costaining is represented by yellow fluorescence. Seen in a and b is one example of three independent experiments, respectively.
Figure Legend Snippet: Detection of iNOS in livers of Con A–treated mice by immunofluorescence staining. ( a ) Con A–induced iNOS expression in livers of BALB/c mice was detected by immunofluorescence staining of liver cryostat sections (rabbit anti-mouse iNOS antiserum, secondary goat anti-rabbit IgG tagged with Cy3; red fluorescence). ( b ) Costaining of iNOS (rabbit anti-mouse iNOS antiserum, secondary swine anti-rabbit IgG tagged with FITC; green fluorescence) and Kupffer cells (BM8, secondary goat anti-rat IgG tagged with Texas red; red fluorescence) 4 hours after Con A or GalN/LPS treatment. All sections were examined by confocal laser-scanning microscopy. Costaining is represented by yellow fluorescence. Seen in a and b is one example of three independent experiments, respectively.

Techniques Used: Mouse Assay, Immunofluorescence, Staining, Expressing, Fluorescence, Confocal Laser Scanning Microscopy

Related Articles

Immunohistochemistry:

Article Title: Differential synchrotron X-ray imaging markers based on the renal microvasculature for tubulointerstitial lesions and glomerulopathy
Article Snippet: .. IHC For IHC, formalin-fixed paraffin-embedded renal sections (2 μm) were stained with biotin-labeled goat anti-Col-III (Southern Biotech, Alabama, USA) or rat anti-CD31 (BD) antibodies overnight at 4 °C, washed with TBST, and incubated with a horseradish peroxidase (HRP)-conjugated rabbit anti-goat IgG or rabbit anti-rat IgG (Dako, Glostrup, DK) antibody for 1 hour. .. The sections were then visualized with 3, 3′-diaminobenzidine (Dako) and counterstained with hematoxylin.

Immunocytochemistry:

Article Title: Exendin-4 attenuates adverse cardiac remodelling in streptozocin-induced diabetes via specific actions on infiltrating macrophages
Article Snippet: .. Immunocytochemistry for CD45 and F4/80 was performed using rat polyclonal (553076, 1:200; BD Bioscience, Oxford, UK) and rat monoclonal (ab6640, 1:200; Abcam, Cambridge, UK) antibodies, respectively, followed by secondary rabbit anti-rat IgG (P0450, 1:100; Dako, Ely, UK) staining, using diaminobenzidine as the chromogen and nuclear counterstaining with haematoxylin. .. Pancreases were harvested into 10 % neutral-buffered formalin, paraffin-embedded, and sectioned (5 μm) prior to staining for glucagon (ab92517, 1:500; Abcam) and insulin (C27C9, 1:200; Cell Signaling, Danvers, MA, USA) using rabbit monoclonal antibodies, followed by incubation with donkey anti-rabbit IgG (ab98502, 1:500; Abcam).

Incubation:

Article Title: Functional Role of the PE Domain and Immunogenicity of the Mycobacterium tuberculosis Triacylglycerol Hydrolase LipY ▿
Article Snippet: .. Thin sections of even thickness (70 nm) were collected on nickel grids and sequentially incubated on drops of (i) PBS containing 5% BSA and 0.1% Tween 20 for 15 min, (ii) rat anti-LipY(ΔPE) antibody for 2 h, (iii) rabbit anti-rat IgG (Dako) for 1 h, and (iv) protein A coupled to gold particles of 5 nm in diameter (PAO) (University of Utrecht, Utrecht, The Netherlands) for 30 min. Antibodies and PAO were diluted in PBS containing 5% BSA and 0.1% Tween 20. ..

Article Title: Differential synchrotron X-ray imaging markers based on the renal microvasculature for tubulointerstitial lesions and glomerulopathy
Article Snippet: .. IHC For IHC, formalin-fixed paraffin-embedded renal sections (2 μm) were stained with biotin-labeled goat anti-Col-III (Southern Biotech, Alabama, USA) or rat anti-CD31 (BD) antibodies overnight at 4 °C, washed with TBST, and incubated with a horseradish peroxidase (HRP)-conjugated rabbit anti-goat IgG or rabbit anti-rat IgG (Dako, Glostrup, DK) antibody for 1 hour. .. The sections were then visualized with 3, 3′-diaminobenzidine (Dako) and counterstained with hematoxylin.

Formalin-fixed Paraffin-Embedded:

Article Title: Differential synchrotron X-ray imaging markers based on the renal microvasculature for tubulointerstitial lesions and glomerulopathy
Article Snippet: .. IHC For IHC, formalin-fixed paraffin-embedded renal sections (2 μm) were stained with biotin-labeled goat anti-Col-III (Southern Biotech, Alabama, USA) or rat anti-CD31 (BD) antibodies overnight at 4 °C, washed with TBST, and incubated with a horseradish peroxidase (HRP)-conjugated rabbit anti-goat IgG or rabbit anti-rat IgG (Dako, Glostrup, DK) antibody for 1 hour. .. The sections were then visualized with 3, 3′-diaminobenzidine (Dako) and counterstained with hematoxylin.

Staining:

Article Title: Hyaluronidase induces a transcapillary pressure gradient and improves the distribution and uptake of liposomal doxorubicin (Caelyx(TM)) in human osteosarcoma xenografts
Article Snippet: .. The vessels were stained using rat anti-mouse CD31 antibody (clone MEC13.3; PharMingen, CA, USA) (16.7 μ g ml− 1 ) for 1 h, followed by either biotinylated goat anti-rat IgG (Vector Lab, CA, USA) (10 μ g ml− 1 ) and streptavidin Alexa Fluor 633 (Molecular Probes, OR, USA) (100 μ g ml− 1 ), when colocalised with doxorubicin, or by rabbit anti-rat IgG (diluted 1 : 200) (DakoCytomation, Glostrup, Denmark), goat anti-rabbit Dako Envision (100 μ l) (DakoCytomation, Glostrup, Denmark) and rabbit anti-goat IgG Alexa Fluor 488 (10 μ g ml− 1 ) (Molecular Probes, Eugene, OR, USA), when colocalised with hyaluronan. .. When staining hyaluronan, the tumour sections were incubated with PBS containing 3% bovine serum albumin for 1 h to prevent nonspecific binding.

Article Title: Differential synchrotron X-ray imaging markers based on the renal microvasculature for tubulointerstitial lesions and glomerulopathy
Article Snippet: .. IHC For IHC, formalin-fixed paraffin-embedded renal sections (2 μm) were stained with biotin-labeled goat anti-Col-III (Southern Biotech, Alabama, USA) or rat anti-CD31 (BD) antibodies overnight at 4 °C, washed with TBST, and incubated with a horseradish peroxidase (HRP)-conjugated rabbit anti-goat IgG or rabbit anti-rat IgG (Dako, Glostrup, DK) antibody for 1 hour. .. The sections were then visualized with 3, 3′-diaminobenzidine (Dako) and counterstained with hematoxylin.

Article Title: Exendin-4 attenuates adverse cardiac remodelling in streptozocin-induced diabetes via specific actions on infiltrating macrophages
Article Snippet: .. Immunocytochemistry for CD45 and F4/80 was performed using rat polyclonal (553076, 1:200; BD Bioscience, Oxford, UK) and rat monoclonal (ab6640, 1:200; Abcam, Cambridge, UK) antibodies, respectively, followed by secondary rabbit anti-rat IgG (P0450, 1:100; Dako, Ely, UK) staining, using diaminobenzidine as the chromogen and nuclear counterstaining with haematoxylin. .. Pancreases were harvested into 10 % neutral-buffered formalin, paraffin-embedded, and sectioned (5 μm) prior to staining for glucagon (ab92517, 1:500; Abcam) and insulin (C27C9, 1:200; Cell Signaling, Danvers, MA, USA) using rabbit monoclonal antibodies, followed by incubation with donkey anti-rabbit IgG (ab98502, 1:500; Abcam).

Immunofluorescence:

Article Title: Inducible nitric oxide synthase is critical for immune-mediated liver injury in mice
Article Snippet: .. Secondary Ab’s for immunofluorescence were goat anti-rabbit IgG tagged with Cy3 (Jackson ImmunoResearch Laboratories Inc.), swine anti-rabbit IgG tagged with FITC (DAKO Corp., Hamburg, Germany), or rabbit anti-rat IgG tagged with Texas red (DAKO Corp.). .. Secondary Ab’s for Western blots were goat anti-rabbit horseradish peroxidase (Dianova) and streptavidin peroxidase (Boehringer Mannheim).

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    Agilent technologies rabbit anti rat igg
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