rabbit anti rat cd86  (Bio-Techne corporation)


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    Bio-Techne corporation rabbit anti rat cd86
    Immunohistochemical assessment of macrophage polarization. Shown are immunohistochemical stainings of the implantation sites of the control (A), adECM (B) and adECM-rGO (C) treatment groups. For each treatment condition, cells positive for M1-marker <t>CD86</t> are stained blue, while cells positive for M2-marker CD163 are stained brown. Higher magnification images (inserts) are represented by dotted line squares. For clarification, blue and yellow arrows represent M1-and M2-positive macrophages, respectively. Regarding adECM-rGO, it is noteworthy that foreign body giant cells (FBGCs) seem to express CD86. Scalebar represents either 50 μm or 10 μm (inserts). Quantification of M2-positive macrophage density in cells permm 2 (n = 6 for each treatment group) is presented in panel D. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Rabbit Anti Rat Cd86, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti rat cd86/product/Bio-Techne corporation
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti rat cd86 - by Bioz Stars, 2024-09
    94/100 stars

    Images

    1) Product Images from "Biocompatible adipose extracellular matrix and reduced graphene oxide nanocomposite for tissue engineering applications"

    Article Title: Biocompatible adipose extracellular matrix and reduced graphene oxide nanocomposite for tissue engineering applications

    Journal: Materials Today Bio

    doi: 10.1016/j.mtbio.2024.101059

    Immunohistochemical assessment of macrophage polarization. Shown are immunohistochemical stainings of the implantation sites of the control (A), adECM (B) and adECM-rGO (C) treatment groups. For each treatment condition, cells positive for M1-marker CD86 are stained blue, while cells positive for M2-marker CD163 are stained brown. Higher magnification images (inserts) are represented by dotted line squares. For clarification, blue and yellow arrows represent M1-and M2-positive macrophages, respectively. Regarding adECM-rGO, it is noteworthy that foreign body giant cells (FBGCs) seem to express CD86. Scalebar represents either 50 μm or 10 μm (inserts). Quantification of M2-positive macrophage density in cells permm 2 (n = 6 for each treatment group) is presented in panel D. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Immunohistochemical assessment of macrophage polarization. Shown are immunohistochemical stainings of the implantation sites of the control (A), adECM (B) and adECM-rGO (C) treatment groups. For each treatment condition, cells positive for M1-marker CD86 are stained blue, while cells positive for M2-marker CD163 are stained brown. Higher magnification images (inserts) are represented by dotted line squares. For clarification, blue and yellow arrows represent M1-and M2-positive macrophages, respectively. Regarding adECM-rGO, it is noteworthy that foreign body giant cells (FBGCs) seem to express CD86. Scalebar represents either 50 μm or 10 μm (inserts). Quantification of M2-positive macrophage density in cells permm 2 (n = 6 for each treatment group) is presented in panel D. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Immunohistochemical staining, Marker, Staining, Clarification Assay

    rabbit anti rat cd86  (Bio-Techne corporation)


    Bioz Verified Symbol Bio-Techne corporation is a verified supplier
    Bioz Manufacturer Symbol Bio-Techne corporation manufactures this product  
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    Bio-Techne corporation rabbit anti rat cd86
    Immunohistochemical assessment of macrophage polarization. Shown are immunohistochemical stainings of the implantation sites of the control (A), adECM (B) and adECM-rGO (C) treatment groups. For each treatment condition, cells positive for M1-marker <t>CD86</t> are stained blue, while cells positive for M2-marker CD163 are stained brown. Higher magnification images (inserts) are represented by dotted line squares. For clarification, blue and yellow arrows represent M1-and M2-positive macrophages, respectively. Regarding adECM-rGO, it is noteworthy that foreign body giant cells (FBGCs) seem to express CD86. Scalebar represents either 50 μm or 10 μm (inserts). Quantification of M2-positive macrophage density in cells permm 2 (n = 6 for each treatment group) is presented in panel D. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Rabbit Anti Rat Cd86, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti rat cd86/product/Bio-Techne corporation
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti rat cd86 - by Bioz Stars, 2024-09
    94/100 stars

    Images

    1) Product Images from "Biocompatible adipose extracellular matrix and reduced graphene oxide nanocomposite for tissue engineering applications"

    Article Title: Biocompatible adipose extracellular matrix and reduced graphene oxide nanocomposite for tissue engineering applications

    Journal: Materials Today Bio

    doi: 10.1016/j.mtbio.2024.101059

    Immunohistochemical assessment of macrophage polarization. Shown are immunohistochemical stainings of the implantation sites of the control (A), adECM (B) and adECM-rGO (C) treatment groups. For each treatment condition, cells positive for M1-marker CD86 are stained blue, while cells positive for M2-marker CD163 are stained brown. Higher magnification images (inserts) are represented by dotted line squares. For clarification, blue and yellow arrows represent M1-and M2-positive macrophages, respectively. Regarding adECM-rGO, it is noteworthy that foreign body giant cells (FBGCs) seem to express CD86. Scalebar represents either 50 μm or 10 μm (inserts). Quantification of M2-positive macrophage density in cells permm 2 (n = 6 for each treatment group) is presented in panel D. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Immunohistochemical assessment of macrophage polarization. Shown are immunohistochemical stainings of the implantation sites of the control (A), adECM (B) and adECM-rGO (C) treatment groups. For each treatment condition, cells positive for M1-marker CD86 are stained blue, while cells positive for M2-marker CD163 are stained brown. Higher magnification images (inserts) are represented by dotted line squares. For clarification, blue and yellow arrows represent M1-and M2-positive macrophages, respectively. Regarding adECM-rGO, it is noteworthy that foreign body giant cells (FBGCs) seem to express CD86. Scalebar represents either 50 μm or 10 μm (inserts). Quantification of M2-positive macrophage density in cells permm 2 (n = 6 for each treatment group) is presented in panel D. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Immunohistochemical staining, Marker, Staining, Clarification Assay

    cd86 rabbit anti rat  (Bioss)


    Bioz Verified Symbol Bioss is a verified supplier
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    Bioss cd86 rabbit anti rat
    Cd86 Rabbit Anti Rat, supplied by Bioss, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd86 rabbit anti rat/product/Bioss
    Average 86 stars, based on 1 article reviews
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    cd86 rabbit anti rat - by Bioz Stars, 2024-09
    86/100 stars

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    rabbit anti rat cd86  (Biorbyt)

     
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    Biorbyt rabbit anti rat cd86
    Rabbit Anti Rat Cd86, supplied by Biorbyt, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti rat cd86/product/Biorbyt
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti rat cd86 - by Bioz Stars, 2024-09
    92/100 stars

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    rabbit anti rat cd86  (Abcam)

     
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    Abcam rabbit anti rat cd86
    Rabbit Anti Rat Cd86, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti rat cd86/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti rat cd86 - by Bioz Stars, 2024-09
    86/100 stars

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    rabbit anti rat cd86  (Abcam)

     
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    Abcam rabbit anti rat cd86
    Effects of EPC-CM on inflammatory cytokine levels in vitro and in vivo . (A) Representative flow cytometry data showing the effect of EPC-CM on BMDMs. (a) Mature BMDMs were defined as CD11b + /F4/80 + subpopulations (upper right), with the purity displayed as percentage of the parent population. (b) Control BMDMs were incubated with CD11b and F4/80 antibody and used to set up the gate. (c) Control BMDMs without LPS stimulation were incubated with anti-rat CD11b, F4/80, <t>CD86</t> and CD206 antibodies. M1 macrophages are CD11b + /F4/80 + /CD86 + /CD206 − (Q1), whereas M2 macrophages are CD11b + /F4/80 + /CD86 − /CD206 + (Q3). (d) BMDMs treated with LPS. (e) BMDMs cultured with Con-M and simultaneously stimulated with LPS. (f) BMDMs cultured with EPC-CM and stimulated with LPS. (B) Quantitation of M1 and M2 cells among the different groups. Compared with the Con-M group, EPC-CM significantly reduced M1 activation, while M2 cells remained relatively unchanged. (C) mRNA expression levels of inflammatory cytokines (optical density ratio) among groups. (D) Immunofluorescence staining for CD86 (M1 marker) and CD206 (M2 marker) in the epicenter 7 days after SCI ( n = 5 per group; green: CD86; red: CD206; blue: DAPI). (E) Quantification of CD86- and CD206-positive cells at 7 days after SCI. ** P < 0.01, vs . PBS group; # P < 0.05, ## P < 0.01, vs . Con-M group. § P < 0.05, §§ P < 0.01, vs . LPS. Data are presented as the mean ± SD (one-way analysis of variance followed by the least significant difference post hoc test). The experiment was performed at least three times. Ctrl: Control; PBS: phosphate-buffered saline; EPC-CM: endothelial progenitor cell–conditioned medium; Con-M: control medium; BMDMs: bone marrow-derived macrophages; LPS: lipopolysaccharide; IL: interleukin; DAPI: 4′,6-diamidino-2-phenylindole.
    Rabbit Anti Rat Cd86, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti rat cd86/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti rat cd86 - by Bioz Stars, 2024-09
    86/100 stars

    Images

    1) Product Images from "Endothelial progenitor cell-conditioned medium promotes angiogenesis and is neuroprotective after spinal cord injury"

    Article Title: Endothelial progenitor cell-conditioned medium promotes angiogenesis and is neuroprotective after spinal cord injury

    Journal: Neural Regeneration Research

    doi: 10.4103/1673-5374.232484

    Effects of EPC-CM on inflammatory cytokine levels in vitro and in vivo . (A) Representative flow cytometry data showing the effect of EPC-CM on BMDMs. (a) Mature BMDMs were defined as CD11b + /F4/80 + subpopulations (upper right), with the purity displayed as percentage of the parent population. (b) Control BMDMs were incubated with CD11b and F4/80 antibody and used to set up the gate. (c) Control BMDMs without LPS stimulation were incubated with anti-rat CD11b, F4/80, CD86 and CD206 antibodies. M1 macrophages are CD11b + /F4/80 + /CD86 + /CD206 − (Q1), whereas M2 macrophages are CD11b + /F4/80 + /CD86 − /CD206 + (Q3). (d) BMDMs treated with LPS. (e) BMDMs cultured with Con-M and simultaneously stimulated with LPS. (f) BMDMs cultured with EPC-CM and stimulated with LPS. (B) Quantitation of M1 and M2 cells among the different groups. Compared with the Con-M group, EPC-CM significantly reduced M1 activation, while M2 cells remained relatively unchanged. (C) mRNA expression levels of inflammatory cytokines (optical density ratio) among groups. (D) Immunofluorescence staining for CD86 (M1 marker) and CD206 (M2 marker) in the epicenter 7 days after SCI ( n = 5 per group; green: CD86; red: CD206; blue: DAPI). (E) Quantification of CD86- and CD206-positive cells at 7 days after SCI. ** P < 0.01, vs . PBS group; # P < 0.05, ## P < 0.01, vs . Con-M group. § P < 0.05, §§ P < 0.01, vs . LPS. Data are presented as the mean ± SD (one-way analysis of variance followed by the least significant difference post hoc test). The experiment was performed at least three times. Ctrl: Control; PBS: phosphate-buffered saline; EPC-CM: endothelial progenitor cell–conditioned medium; Con-M: control medium; BMDMs: bone marrow-derived macrophages; LPS: lipopolysaccharide; IL: interleukin; DAPI: 4′,6-diamidino-2-phenylindole.
    Figure Legend Snippet: Effects of EPC-CM on inflammatory cytokine levels in vitro and in vivo . (A) Representative flow cytometry data showing the effect of EPC-CM on BMDMs. (a) Mature BMDMs were defined as CD11b + /F4/80 + subpopulations (upper right), with the purity displayed as percentage of the parent population. (b) Control BMDMs were incubated with CD11b and F4/80 antibody and used to set up the gate. (c) Control BMDMs without LPS stimulation were incubated with anti-rat CD11b, F4/80, CD86 and CD206 antibodies. M1 macrophages are CD11b + /F4/80 + /CD86 + /CD206 − (Q1), whereas M2 macrophages are CD11b + /F4/80 + /CD86 − /CD206 + (Q3). (d) BMDMs treated with LPS. (e) BMDMs cultured with Con-M and simultaneously stimulated with LPS. (f) BMDMs cultured with EPC-CM and stimulated with LPS. (B) Quantitation of M1 and M2 cells among the different groups. Compared with the Con-M group, EPC-CM significantly reduced M1 activation, while M2 cells remained relatively unchanged. (C) mRNA expression levels of inflammatory cytokines (optical density ratio) among groups. (D) Immunofluorescence staining for CD86 (M1 marker) and CD206 (M2 marker) in the epicenter 7 days after SCI ( n = 5 per group; green: CD86; red: CD206; blue: DAPI). (E) Quantification of CD86- and CD206-positive cells at 7 days after SCI. ** P < 0.01, vs . PBS group; # P < 0.05, ## P < 0.01, vs . Con-M group. § P < 0.05, §§ P < 0.01, vs . LPS. Data are presented as the mean ± SD (one-way analysis of variance followed by the least significant difference post hoc test). The experiment was performed at least three times. Ctrl: Control; PBS: phosphate-buffered saline; EPC-CM: endothelial progenitor cell–conditioned medium; Con-M: control medium; BMDMs: bone marrow-derived macrophages; LPS: lipopolysaccharide; IL: interleukin; DAPI: 4′,6-diamidino-2-phenylindole.

    Techniques Used: In Vitro, In Vivo, Flow Cytometry, Incubation, Cell Culture, Quantitation Assay, Activation Assay, Expressing, Immunofluorescence, Staining, Marker, Derivative Assay

    rabbit anti rat cd86  (Abcam)

     
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    Abcam rabbit anti rat cd86
    The effect of aspirin administration on overall macrophage phenotype in vivo during UBM mediated constructive remodeling. Tissue sections from the specified time points were immunolabeled for CD68 (pan-macrophage, orange), CD206 (M2, green), and <t>CD86</t> (M1, red) and imaged. Four representative images of each tissue section were collected and the number of CD68+CD206+ and CD68+CD86+ cells were quantified using Cell Profiler. (A) Representative images of tissue sections from untreated and aspirin treated animals are shown. (B) The mean M2/M1 ratio in the tissue sections is expressed as a ratio of CD68+CD206+ cells to CD68+CD86+ cells. #, a significant main effect (averaged data over all time points) for the untreated vs. aspirin treated groups is shown (p<0.05). *, significant differences in M2/M1 ratio (p<0.05) are shown.
    Rabbit Anti Rat Cd86, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti rat cd86/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti rat cd86 - by Bioz Stars, 2024-09
    86/100 stars

    Images

    1) Product Images from "Inhibition of COX1/2 alters the host response and reduces ECM scaffold mediated constructive tissue remodeling in a rodent model of skeletal muscle injury"

    Article Title: Inhibition of COX1/2 alters the host response and reduces ECM scaffold mediated constructive tissue remodeling in a rodent model of skeletal muscle injury

    Journal: Acta biomaterialia

    doi: 10.1016/j.actbio.2015.11.043

    The effect of aspirin administration on overall macrophage phenotype in vivo during UBM mediated constructive remodeling. Tissue sections from the specified time points were immunolabeled for CD68 (pan-macrophage, orange), CD206 (M2, green), and CD86 (M1, red) and imaged. Four representative images of each tissue section were collected and the number of CD68+CD206+ and CD68+CD86+ cells were quantified using Cell Profiler. (A) Representative images of tissue sections from untreated and aspirin treated animals are shown. (B) The mean M2/M1 ratio in the tissue sections is expressed as a ratio of CD68+CD206+ cells to CD68+CD86+ cells. #, a significant main effect (averaged data over all time points) for the untreated vs. aspirin treated groups is shown (p<0.05). *, significant differences in M2/M1 ratio (p<0.05) are shown.
    Figure Legend Snippet: The effect of aspirin administration on overall macrophage phenotype in vivo during UBM mediated constructive remodeling. Tissue sections from the specified time points were immunolabeled for CD68 (pan-macrophage, orange), CD206 (M2, green), and CD86 (M1, red) and imaged. Four representative images of each tissue section were collected and the number of CD68+CD206+ and CD68+CD86+ cells were quantified using Cell Profiler. (A) Representative images of tissue sections from untreated and aspirin treated animals are shown. (B) The mean M2/M1 ratio in the tissue sections is expressed as a ratio of CD68+CD206+ cells to CD68+CD86+ cells. #, a significant main effect (averaged data over all time points) for the untreated vs. aspirin treated groups is shown (p<0.05). *, significant differences in M2/M1 ratio (p<0.05) are shown.

    Techniques Used: In Vivo, Immunolabeling

    The effect of aspirin treatment on CD206 and CD86 expression in macrophages treated with UBM hydrogel in vitro. THP1 monocytes were differentiated to a macrophage-like cell lineage with PMA for 24 hours and rested for an additional 24 hours. Aspirin (200 μM) was added to cells for 1 hour prior to UBM hydrogel (0.5 mg/mL) addition. Cell lysates were collected at 4, 8, and 24 hours, resolved on SDS gels, transferred to membranes, and immunoblotted for CD206 and CD86 expression. Actin served as a loading control. (A) Representative blot of CD206 expression and (B) relative changes in CD206 expression compared to 0 hr control measured with densitometry. (C) Representative blot of CD86 expression and (D) relative changes in CD86 expression compared to 0 hr control measured with densitometry. Significant differences in CD206 and CD86 expression between aspirin treated and untreated cells are shown (p<0.05).
    Figure Legend Snippet: The effect of aspirin treatment on CD206 and CD86 expression in macrophages treated with UBM hydrogel in vitro. THP1 monocytes were differentiated to a macrophage-like cell lineage with PMA for 24 hours and rested for an additional 24 hours. Aspirin (200 μM) was added to cells for 1 hour prior to UBM hydrogel (0.5 mg/mL) addition. Cell lysates were collected at 4, 8, and 24 hours, resolved on SDS gels, transferred to membranes, and immunoblotted for CD206 and CD86 expression. Actin served as a loading control. (A) Representative blot of CD206 expression and (B) relative changes in CD206 expression compared to 0 hr control measured with densitometry. (C) Representative blot of CD86 expression and (D) relative changes in CD86 expression compared to 0 hr control measured with densitometry. Significant differences in CD206 and CD86 expression between aspirin treated and untreated cells are shown (p<0.05).

    Techniques Used: Expressing, In Vitro

    rabbit anti rat cd86  (Abcam)

     
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    Structured Review

    Abcam rabbit anti rat cd86
    The effect of aspirin administration on overall macrophage phenotype in vivo during UBM mediated constructive remodeling. Tissue sections from the specified time points were immunolabeled for CD68 (pan-macrophage, orange), CD206 (M2, green), and <t>CD86</t> (M1, red) and imaged. Four representative images of each tissue section were collected and the number of CD68+CD206+ and CD68+CD86+ cells were quantified using Cell Profiler. (A) Representative images of tissue sections from untreated and aspirin treated animals are shown. (B) The mean M2/M1 ratio in the tissue sections is expressed as a ratio of CD68+CD206+ cells to CD68+CD86+ cells. #, a significant main effect (averaged data over all time points) for the untreated vs. aspirin treated groups is shown (p<0.05). *, significant differences in M2/M1 ratio (p<0.05) are shown.
    Rabbit Anti Rat Cd86, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti rat cd86/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti rat cd86 - by Bioz Stars, 2024-09
    86/100 stars

    Images

    1) Product Images from "Inhibition of COX1/2 alters the host response and reduces ECM scaffold mediated constructive tissue remodeling in a rodent model of skeletal muscle injury"

    Article Title: Inhibition of COX1/2 alters the host response and reduces ECM scaffold mediated constructive tissue remodeling in a rodent model of skeletal muscle injury

    Journal: Acta biomaterialia

    doi: 10.1016/j.actbio.2015.11.043

    The effect of aspirin administration on overall macrophage phenotype in vivo during UBM mediated constructive remodeling. Tissue sections from the specified time points were immunolabeled for CD68 (pan-macrophage, orange), CD206 (M2, green), and CD86 (M1, red) and imaged. Four representative images of each tissue section were collected and the number of CD68+CD206+ and CD68+CD86+ cells were quantified using Cell Profiler. (A) Representative images of tissue sections from untreated and aspirin treated animals are shown. (B) The mean M2/M1 ratio in the tissue sections is expressed as a ratio of CD68+CD206+ cells to CD68+CD86+ cells. #, a significant main effect (averaged data over all time points) for the untreated vs. aspirin treated groups is shown (p<0.05). *, significant differences in M2/M1 ratio (p<0.05) are shown.
    Figure Legend Snippet: The effect of aspirin administration on overall macrophage phenotype in vivo during UBM mediated constructive remodeling. Tissue sections from the specified time points were immunolabeled for CD68 (pan-macrophage, orange), CD206 (M2, green), and CD86 (M1, red) and imaged. Four representative images of each tissue section were collected and the number of CD68+CD206+ and CD68+CD86+ cells were quantified using Cell Profiler. (A) Representative images of tissue sections from untreated and aspirin treated animals are shown. (B) The mean M2/M1 ratio in the tissue sections is expressed as a ratio of CD68+CD206+ cells to CD68+CD86+ cells. #, a significant main effect (averaged data over all time points) for the untreated vs. aspirin treated groups is shown (p<0.05). *, significant differences in M2/M1 ratio (p<0.05) are shown.

    Techniques Used: In Vivo, Immunolabeling

    The effect of aspirin treatment on CD206 and CD86 expression in macrophages treated with UBM hydrogel in vitro. THP1 monocytes were differentiated to a macrophage-like cell lineage with PMA for 24 hours and rested for an additional 24 hours. Aspirin (200 μM) was added to cells for 1 hour prior to UBM hydrogel (0.5 mg/mL) addition. Cell lysates were collected at 4, 8, and 24 hours, resolved on SDS gels, transferred to membranes, and immunoblotted for CD206 and CD86 expression. Actin served as a loading control. (A) Representative blot of CD206 expression and (B) relative changes in CD206 expression compared to 0 hr control measured with densitometry. (C) Representative blot of CD86 expression and (D) relative changes in CD86 expression compared to 0 hr control measured with densitometry. Significant differences in CD206 and CD86 expression between aspirin treated and untreated cells are shown (p<0.05).
    Figure Legend Snippet: The effect of aspirin treatment on CD206 and CD86 expression in macrophages treated with UBM hydrogel in vitro. THP1 monocytes were differentiated to a macrophage-like cell lineage with PMA for 24 hours and rested for an additional 24 hours. Aspirin (200 μM) was added to cells for 1 hour prior to UBM hydrogel (0.5 mg/mL) addition. Cell lysates were collected at 4, 8, and 24 hours, resolved on SDS gels, transferred to membranes, and immunoblotted for CD206 and CD86 expression. Actin served as a loading control. (A) Representative blot of CD206 expression and (B) relative changes in CD206 expression compared to 0 hr control measured with densitometry. (C) Representative blot of CD86 expression and (D) relative changes in CD86 expression compared to 0 hr control measured with densitometry. Significant differences in CD206 and CD86 expression between aspirin treated and untreated cells are shown (p<0.05).

    Techniques Used: Expressing, In Vitro

    rabbit anti rat cd86  (Abcam)

     
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    Structured Review

    Abcam rabbit anti rat cd86
    The effect of aspirin administration on overall macrophage phenotype in vivo during UBM mediated constructive remodeling. Tissue sections from the specified time points were immunolabeled for CD68 (pan-macrophage, orange), CD206 (M2, green), and <t>CD86</t> (M1, red) and imaged. Four representative images of each tissue section were collected and the number of CD68+CD206+ and CD68+CD86+ cells were quantified using Cell Profiler. (A) Representative images of tissue sections from untreated and aspirin treated animals are shown. (B) The mean M2/M1 ratio in the tissue sections is expressed as a ratio of CD68+CD206+ cells to CD68+CD86+ cells. #, a significant main effect (averaged data over all time points) for the untreated vs. aspirin treated groups is shown (p<0.05). *, significant differences in M2/M1 ratio (p<0.05) are shown.
    Rabbit Anti Rat Cd86, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti rat cd86/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti rat cd86 - by Bioz Stars, 2024-09
    86/100 stars

    Images

    1) Product Images from "Inhibition of COX1/2 alters the host response and reduces ECM scaffold mediated constructive tissue remodeling in a rodent model of skeletal muscle injury"

    Article Title: Inhibition of COX1/2 alters the host response and reduces ECM scaffold mediated constructive tissue remodeling in a rodent model of skeletal muscle injury

    Journal: Acta biomaterialia

    doi: 10.1016/j.actbio.2015.11.043

    The effect of aspirin administration on overall macrophage phenotype in vivo during UBM mediated constructive remodeling. Tissue sections from the specified time points were immunolabeled for CD68 (pan-macrophage, orange), CD206 (M2, green), and CD86 (M1, red) and imaged. Four representative images of each tissue section were collected and the number of CD68+CD206+ and CD68+CD86+ cells were quantified using Cell Profiler. (A) Representative images of tissue sections from untreated and aspirin treated animals are shown. (B) The mean M2/M1 ratio in the tissue sections is expressed as a ratio of CD68+CD206+ cells to CD68+CD86+ cells. #, a significant main effect (averaged data over all time points) for the untreated vs. aspirin treated groups is shown (p<0.05). *, significant differences in M2/M1 ratio (p<0.05) are shown.
    Figure Legend Snippet: The effect of aspirin administration on overall macrophage phenotype in vivo during UBM mediated constructive remodeling. Tissue sections from the specified time points were immunolabeled for CD68 (pan-macrophage, orange), CD206 (M2, green), and CD86 (M1, red) and imaged. Four representative images of each tissue section were collected and the number of CD68+CD206+ and CD68+CD86+ cells were quantified using Cell Profiler. (A) Representative images of tissue sections from untreated and aspirin treated animals are shown. (B) The mean M2/M1 ratio in the tissue sections is expressed as a ratio of CD68+CD206+ cells to CD68+CD86+ cells. #, a significant main effect (averaged data over all time points) for the untreated vs. aspirin treated groups is shown (p<0.05). *, significant differences in M2/M1 ratio (p<0.05) are shown.

    Techniques Used: In Vivo, Immunolabeling

    The effect of aspirin treatment on CD206 and CD86 expression in macrophages treated with UBM hydrogel in vitro. THP1 monocytes were differentiated to a macrophage-like cell lineage with PMA for 24 hours and rested for an additional 24 hours. Aspirin (200 μM) was added to cells for 1 hour prior to UBM hydrogel (0.5 mg/mL) addition. Cell lysates were collected at 4, 8, and 24 hours, resolved on SDS gels, transferred to membranes, and immunoblotted for CD206 and CD86 expression. Actin served as a loading control. (A) Representative blot of CD206 expression and (B) relative changes in CD206 expression compared to 0 hr control measured with densitometry. (C) Representative blot of CD86 expression and (D) relative changes in CD86 expression compared to 0 hr control measured with densitometry. Significant differences in CD206 and CD86 expression between aspirin treated and untreated cells are shown (p<0.05).
    Figure Legend Snippet: The effect of aspirin treatment on CD206 and CD86 expression in macrophages treated with UBM hydrogel in vitro. THP1 monocytes were differentiated to a macrophage-like cell lineage with PMA for 24 hours and rested for an additional 24 hours. Aspirin (200 μM) was added to cells for 1 hour prior to UBM hydrogel (0.5 mg/mL) addition. Cell lysates were collected at 4, 8, and 24 hours, resolved on SDS gels, transferred to membranes, and immunoblotted for CD206 and CD86 expression. Actin served as a loading control. (A) Representative blot of CD206 expression and (B) relative changes in CD206 expression compared to 0 hr control measured with densitometry. (C) Representative blot of CD86 expression and (D) relative changes in CD86 expression compared to 0 hr control measured with densitometry. Significant differences in CD206 and CD86 expression between aspirin treated and untreated cells are shown (p<0.05).

    Techniques Used: Expressing, In Vitro

    rabbit anti rat cd86  (Abcam)

     
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    Abcam rabbit anti rat cd86
    Rabbit Anti Rat Cd86, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Techne corporation rabbit anti rat cd86
    Immunohistochemical assessment of macrophage polarization. Shown are immunohistochemical stainings of the implantation sites of the control (A), adECM (B) and adECM-rGO (C) treatment groups. For each treatment condition, cells positive for M1-marker <t>CD86</t> are stained blue, while cells positive for M2-marker CD163 are stained brown. Higher magnification images (inserts) are represented by dotted line squares. For clarification, blue and yellow arrows represent M1-and M2-positive macrophages, respectively. Regarding adECM-rGO, it is noteworthy that foreign body giant cells (FBGCs) seem to express CD86. Scalebar represents either 50 μm or 10 μm (inserts). Quantification of M2-positive macrophage density in cells permm 2 (n = 6 for each treatment group) is presented in panel D. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    cd86 rabbit anti rat  (Bioss)
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    Immunohistochemical assessment of macrophage polarization. Shown are immunohistochemical stainings of the implantation sites of the control (A), adECM (B) and adECM-rGO (C) treatment groups. For each treatment condition, cells positive for M1-marker <t>CD86</t> are stained blue, while cells positive for M2-marker CD163 are stained brown. Higher magnification images (inserts) are represented by dotted line squares. For clarification, blue and yellow arrows represent M1-and M2-positive macrophages, respectively. Regarding adECM-rGO, it is noteworthy that foreign body giant cells (FBGCs) seem to express CD86. Scalebar represents either 50 μm or 10 μm (inserts). Quantification of M2-positive macrophage density in cells permm 2 (n = 6 for each treatment group) is presented in panel D. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Cd86 Rabbit Anti Rat, supplied by Bioss, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti rat cd86  (Biorbyt)
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    Immunohistochemical assessment of macrophage polarization. Shown are immunohistochemical stainings of the implantation sites of the control (A), adECM (B) and adECM-rGO (C) treatment groups. For each treatment condition, cells positive for M1-marker <t>CD86</t> are stained blue, while cells positive for M2-marker CD163 are stained brown. Higher magnification images (inserts) are represented by dotted line squares. For clarification, blue and yellow arrows represent M1-and M2-positive macrophages, respectively. Regarding adECM-rGO, it is noteworthy that foreign body giant cells (FBGCs) seem to express CD86. Scalebar represents either 50 μm or 10 μm (inserts). Quantification of M2-positive macrophage density in cells permm 2 (n = 6 for each treatment group) is presented in panel D. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    rabbit anti rat cd86  (Abcam)
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    Immunohistochemical assessment of macrophage polarization. Shown are immunohistochemical stainings of the implantation sites of the control (A), adECM (B) and adECM-rGO (C) treatment groups. For each treatment condition, cells positive for M1-marker <t>CD86</t> are stained blue, while cells positive for M2-marker CD163 are stained brown. Higher magnification images (inserts) are represented by dotted line squares. For clarification, blue and yellow arrows represent M1-and M2-positive macrophages, respectively. Regarding adECM-rGO, it is noteworthy that foreign body giant cells (FBGCs) seem to express CD86. Scalebar represents either 50 μm or 10 μm (inserts). Quantification of M2-positive macrophage density in cells permm 2 (n = 6 for each treatment group) is presented in panel D. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    Immunohistochemical assessment of macrophage polarization. Shown are immunohistochemical stainings of the implantation sites of the control (A), adECM (B) and adECM-rGO (C) treatment groups. For each treatment condition, cells positive for M1-marker CD86 are stained blue, while cells positive for M2-marker CD163 are stained brown. Higher magnification images (inserts) are represented by dotted line squares. For clarification, blue and yellow arrows represent M1-and M2-positive macrophages, respectively. Regarding adECM-rGO, it is noteworthy that foreign body giant cells (FBGCs) seem to express CD86. Scalebar represents either 50 μm or 10 μm (inserts). Quantification of M2-positive macrophage density in cells permm 2 (n = 6 for each treatment group) is presented in panel D. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Biocompatible adipose extracellular matrix and reduced graphene oxide nanocomposite for tissue engineering applications

    doi: 10.1016/j.mtbio.2024.101059

    Figure Lengend Snippet: Immunohistochemical assessment of macrophage polarization. Shown are immunohistochemical stainings of the implantation sites of the control (A), adECM (B) and adECM-rGO (C) treatment groups. For each treatment condition, cells positive for M1-marker CD86 are stained blue, while cells positive for M2-marker CD163 are stained brown. Higher magnification images (inserts) are represented by dotted line squares. For clarification, blue and yellow arrows represent M1-and M2-positive macrophages, respectively. Regarding adECM-rGO, it is noteworthy that foreign body giant cells (FBGCs) seem to express CD86. Scalebar represents either 50 μm or 10 μm (inserts). Quantification of M2-positive macrophage density in cells permm 2 (n = 6 for each treatment group) is presented in panel D. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Upon peroxidase (PO) detection with H 2 O 2 and 3,3′-diaminobenzidine (DAB, Sigma-Aldrich) for 10 min, sections were incubated with rabbit anti-rat CD86 (1:800; NBP2-67417, Bio-Techne, Abingdon, UK), overnight at 4 °C.

    Techniques: Immunohistochemical staining, Marker, Staining, Clarification Assay