Journal: Journal of Xenobiotics

Article Title: Chronic Exposure to Both Electronic and Conventional Cigarettes Alters Ileum and Colon Turnover, Immune Function, and Barrier Integrity in Mice

doi: 10.3390/jox14030053

Figure Lengend Snippet: Effects of chronic electronic and conventional cigarette exposure on ileum- and colon-proliferation signalling pathways. Transcript levels of markers of major proliferation pathways in ileum ( A ) and colon ( B ). n = 14 per group. Transcript levels of cyclin D1 pathway genes in ileum ( C ) and colon ( D ). n = 14 per group. Cyclin D1 immunostaining, with higher magnification on the right, and quantification of cyclin D1 mean optical density in ileum ( E ) and colon ( F ). Cyclin D1 stain: brown. Nuclear stain: blue. n = 5 per group. * p < 0.05, ** p < 0.01, *** p < 0.005 and **** p < 0.001 compared to the control group (Air), as determined by the Mann–Whitney U test.

Article Snippet: Rabbit anti-mouse Ki-67 primary antibody (MA5-14520, Invitrogen; Fisher Scientific, Illkirch, France 1/100 dilution), rabbit anti-mouse cyclin D1 primary antibody (#55506, Cell Signaling Technology; Danvers, USA 1/400 dilution), rabbit anti-mouse ZO-1 primary antibody (#61-7300, Invitrogen; 1/200 dilution), and rabbit anti-mouse occludin primary antibody (#91131, Cell Signaling; 1/200 dilution) were incubated overnight at 4 °C.

Techniques: Immunostaining, Staining, Control, MANN-WHITNEY

Journal: OncoTargets and therapy

Article Title: A novel synthetic Asiatic acid derivative induces apoptosis and inhibits proliferation and mobility of gastric cancer cells by suppressing STAT3 signaling pathway

doi: 10.2147/OTT.S121619

Figure Lengend Snippet: Transfection with STAT3 shRNA and STAT3 expression plasmid in SGC7901 modulated the expression of proteins, which were induced by AA-PMe. Notes: SGC7901 cells (2×10 6 /mL) were transfected with either shSTAT3 or STAT3 plasmid. After 24 h, cells were treated with 5, 10, and 50 μM AA-PMe for another 24 h and the whole-cell extracts were subjected to Western blot analysis for STAT3, pSTAT3, Bax, Bcl-2, c-Myc, cyclin D1, and MMP-2, and MMP-9 ( A ). The density of A was analyzed according to GAPDH ( B ). Representative of three independent experiments. Significant differences are denoted by * P <0.05, ** P <0.01, and *** P <0.001. Abbreviations: AA-PMe, asiatic acid- N -(2α,3β,23-acetoxyurs-12-en-28-oyl)-L-proline methyl ester; STAT3, signal transducers and activators of transcription 3; MMP, matrix metalloproteinase.

Article Snippet: Rabbit polyclonal primary antibodies to phospho-STAT3(Tyr 705 ), JAK2, phospho-JAK2, c-Myc, MMP-2, and MMP-9, and mouse monoclonal primary antibody against cyclin D1 were all obtained from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Transfection, shRNA, Expressing, Plasmid Preparation, Western Blot