rabbit anti-mcherry 600–401-p16  (Rockland Immunochemicals)

Journal: Frontiers in Neuroscience

Article Title: Chemogenetic tools for modulation of spatial learning in dopamine transporter deficient rats

doi: 10.3389/fnins.2025.1615208

Figure Lengend Snippet: Immunofluorescence staining of rat brain LC neurons after administration of CAV PRS hM3D(Gq)-mCherry virus to the PFC (A,B) ; green fluorescence is DβH-positive neurons (C) , red fluorescence is mCherry-positive neurons (D) , double-labeled neurons appear yellow-orange (E) .

Article Snippet: Then, the sections were incubated in the primary antibodies: mouse anti-DβH antibody (1:2000, MAB308, Chemicon) and rabbit anti-mCherry antibody (1:1000 Cat#632496, Takara Bio, United States) ( ) for 24 h at RT, and the secondary antibody: donkey anti-mouse IgG Alexa Fluor 488 (1:500, abcam, ab150105, UK) and CyTM3 AffiniPure donkey anti-rabbit IgG (1:800, AB_2307443, Jackson ImmunoResearch Labs, United States) for 2 h at 37°C.

Techniques: Immunofluorescence, Staining, Virus, Fluorescence, Labeling

Journal: Cells

Article Title: Preclinical Assessment in Juvenile Sheep of an Allogeneic Bone Tissue Engineering Product with Wharton’s Jelly Mesenchymal Stromal Cells

doi: 10.3390/cells14120862

Figure Lengend Snippet: Characterisation of mCherry-WJ-MSCs and biocompatibility with synthetic scaffolds. ( A ) Phenotypic characterisation of mCherry-labelled WJ-MSCs showing the expression of positive (CD44 and CD166) and negative (CD31 and MHCII) surface markers. ( B ) Representative images showing the appearance of mCherry-WJ-MSC cultures and their trilineage differentiation potential. Scale bars: 100 µm. ( C ) Representative images of live/dead staining performed on mCherry-WJ-MSCs combined with synthetic scaffolds at days 0, 3, and 7. Merged images, as well as split green and red fluorescence channels, are shown. Scale bars: 100 µm.

Article Snippet: Samples were then incubated with the following primary and secondary antibodies at dilutions of 1:250 and 1:500, respectively: rabbit polyclonal anti-mCherry antibody (NBP2-25157, Novus Biologicals, LLC, Centennial, CO, USA) and goat anti-rabbit IgG (HRP) (31460, ThermoFisher Scientific Inc., Waltham, MA, USA).

Techniques: Expressing, Staining, Fluorescence

Journal: Cells

Article Title: Preclinical Assessment in Juvenile Sheep of an Allogeneic Bone Tissue Engineering Product with Wharton’s Jelly Mesenchymal Stromal Cells

doi: 10.3390/cells14120862

Figure Lengend Snippet: Persistence of mCherry-WJ-MSCs in the defect site and gonads. ( A ) Representative images of mCherry immunostaining in histological sections of mCherry-WJ-MSC-laden fibrin-based hydrogel (control) and 6-week-treated bones (group 2 and group 4). Black arrows indicate positive cells. Scale bars: 100 µm. ( B ) Results of PCR amplification of the mCherry sequence in the gonads of animals treated with cellular TEPs (group 2 (G2) and group 4 (G4)). DNA from mCherry-WJ-MSC cultures (C+), water (C−), and DNA from the gonads of a sheep treated with acellular TEP (group 3 (G3)) were included as controls. Band size: 288 pb. DNA ladder: BrightMAX™ 100–2000 bp (L0015, Canvax).

Article Snippet: Samples were then incubated with the following primary and secondary antibodies at dilutions of 1:250 and 1:500, respectively: rabbit polyclonal anti-mCherry antibody (NBP2-25157, Novus Biologicals, LLC, Centennial, CO, USA) and goat anti-rabbit IgG (HRP) (31460, ThermoFisher Scientific Inc., Waltham, MA, USA).

Techniques: Immunostaining, Control, Amplification, Sequencing