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Structured Review

Abcam rabbit anti human sumo 1
A) Representative confocal micrographs showing the nuclear and cytoplasmatic expression of human POLR2E and <t>SUMO-1</t> proteins in vivo, in kidney sections of cisplatin-AKI (AKI-CIS) mice treated or not with MVs and sacrificed 48 hours later, and in vitro by TECs treated with cisplatin and cultured in the absence (vehicle) or in the presence of 50 µg of MVs (MV) for 24 hours. Nuclei were counterstained with Hoechst dye. Original magnification: ×400 for kidney sections and ×630 for TECs. B) 1×10 5 TECs treated with cisplatin and cultured in the absence (CIS) or in the presence of two different preparations of MVs (+MV) for 1 hour were analysed by RT-PCR for specific human mRNA POLR2E . Bands of PCR products specific for human POLR2E of the expected size (90 pb) were detected in a 4% agarose gel electrophoresis. As positive control the extract of human bone marrow-derived MSCs (BM-MSC) was used. The * indicates the control without cDNA.
Rabbit Anti Human Sumo 1, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
rabbit anti human sumo 1 - by Bioz Stars, 2025-02
86/100 stars

Images

1) Product Images from "Microvesicles Derived from Mesenchymal Stem Cells Enhance Survival in a Lethal Model of Acute Kidney Injury"

Article Title: Microvesicles Derived from Mesenchymal Stem Cells Enhance Survival in a Lethal Model of Acute Kidney Injury

Journal: PLoS ONE

doi: 10.1371/journal.pone.0033115

A) Representative confocal micrographs showing the nuclear and cytoplasmatic expression of human POLR2E and SUMO-1 proteins in vivo, in kidney sections of cisplatin-AKI (AKI-CIS) mice treated or not with MVs and sacrificed 48 hours later, and in vitro by TECs treated with cisplatin and cultured in the absence (vehicle) or in the presence of 50 µg of MVs (MV) for 24 hours. Nuclei were counterstained with Hoechst dye. Original magnification: ×400 for kidney sections and ×630 for TECs. B) 1×10 5 TECs treated with cisplatin and cultured in the absence (CIS) or in the presence of two different preparations of MVs (+MV) for 1 hour were analysed by RT-PCR for specific human mRNA POLR2E . Bands of PCR products specific for human POLR2E of the expected size (90 pb) were detected in a 4% agarose gel electrophoresis. As positive control the extract of human bone marrow-derived MSCs (BM-MSC) was used. The * indicates the control without cDNA.
Figure Legend Snippet: A) Representative confocal micrographs showing the nuclear and cytoplasmatic expression of human POLR2E and SUMO-1 proteins in vivo, in kidney sections of cisplatin-AKI (AKI-CIS) mice treated or not with MVs and sacrificed 48 hours later, and in vitro by TECs treated with cisplatin and cultured in the absence (vehicle) or in the presence of 50 µg of MVs (MV) for 24 hours. Nuclei were counterstained with Hoechst dye. Original magnification: ×400 for kidney sections and ×630 for TECs. B) 1×10 5 TECs treated with cisplatin and cultured in the absence (CIS) or in the presence of two different preparations of MVs (+MV) for 1 hour were analysed by RT-PCR for specific human mRNA POLR2E . Bands of PCR products specific for human POLR2E of the expected size (90 pb) were detected in a 4% agarose gel electrophoresis. As positive control the extract of human bone marrow-derived MSCs (BM-MSC) was used. The * indicates the control without cDNA.

Techniques Used: Expressing, In Vivo, In Vitro, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, Positive Control, Derivative Assay

A) Representative MV size analyses by direct measurement with NTA, showing no difference among MVs treated or not with RNase. B) Representative Bioanalyzer profile, showing the size distribution of total RNA extracted from MVs treated or not with RNAse. The first peak (left side of each panel) represents an internal standard. The two peaks in Sample 1 (black arrows) represent 18 S (left) and 28 S (right) ribosomal RNA, only partially detectable in MVs. The red arrows showed the reduction of 18 and 28 S fragment inside RNAse-treated MVs. C) Histogram showing the expression level of SUMO-1 , POLR2 and Act B transcripts in MVs treated or not with RNase, express as 2 -δCt , as described in material and methods.
Figure Legend Snippet: A) Representative MV size analyses by direct measurement with NTA, showing no difference among MVs treated or not with RNase. B) Representative Bioanalyzer profile, showing the size distribution of total RNA extracted from MVs treated or not with RNAse. The first peak (left side of each panel) represents an internal standard. The two peaks in Sample 1 (black arrows) represent 18 S (left) and 28 S (right) ribosomal RNA, only partially detectable in MVs. The red arrows showed the reduction of 18 and 28 S fragment inside RNAse-treated MVs. C) Histogram showing the expression level of SUMO-1 , POLR2 and Act B transcripts in MVs treated or not with RNase, express as 2 -δCt , as described in material and methods.

Techniques Used: Expressing



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Boster Bio rabbit anti sumo 1
In order to illustrate the preferentially activity of SENP3 to <t>SUMO-1</t> or SUMO-2/3 mediated SUMOylation profile, quantitative immunoblots of SUMO-1 and its conjugates ( A ) as well as SUMO-2/3 and its conjugates ( B ) were measured in control and SENP3 RNAi GV oocytes. To study the effect of SENP3 RNAi on the localization of SUMO-1 and SUMO-2/3, control or SENP3 RNAi GV oocytes were immunostained for SENP3 and SUMO-1 ( C ) or SUMO-2/3 ( D ). Note that neither SUMO-1 nor SUMO-2/3 localization pattern was altered after SENP3 knockdown. SENP3, red; SUMO-1 and SUMO-2/3, green; DNA, blue. Bar = 10 μm.
Rabbit Anti Sumo 1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti sumo 1/product/Boster Bio
Average 90 stars, based on 1 article reviews
rabbit anti sumo 1 - by Bioz Stars, 2025-02
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Abcam rabbit anti human sumo 1
A) Representative confocal micrographs showing the nuclear and cytoplasmatic expression of human POLR2E and <t>SUMO-1</t> proteins in vivo, in kidney sections of cisplatin-AKI (AKI-CIS) mice treated or not with MVs and sacrificed 48 hours later, and in vitro by TECs treated with cisplatin and cultured in the absence (vehicle) or in the presence of 50 µg of MVs (MV) for 24 hours. Nuclei were counterstained with Hoechst dye. Original magnification: ×400 for kidney sections and ×630 for TECs. B) 1×10 5 TECs treated with cisplatin and cultured in the absence (CIS) or in the presence of two different preparations of MVs (+MV) for 1 hour were analysed by RT-PCR for specific human mRNA POLR2E . Bands of PCR products specific for human POLR2E of the expected size (90 pb) were detected in a 4% agarose gel electrophoresis. As positive control the extract of human bone marrow-derived MSCs (BM-MSC) was used. The * indicates the control without cDNA.
Rabbit Anti Human Sumo 1, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti human sumo 1/product/Abcam
Average 86 stars, based on 1 article reviews
rabbit anti human sumo 1 - by Bioz Stars, 2025-02
86/100 stars
  Buy from Supplier

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Cell Signaling Technology Inc rabbit anti human sumo 1
<t>SUMO-1</t> and SUMO-2,3 protein expression. Dot blot analysis of global SUMO-1 expressions are shown in ( a ) for young and old ASCs, and ( e ) for young and old fibroblasts. Treatment conditions were: ctrl – at baseline without treatment; HS -heat shock at 42 °C for 1 h and HS/Rec: heat shock at 42 °C for 1 h followed by 1 h recovery at 37 °C. The intensities of the dots were quantified using ImageJ. Average intensities of the groups are plotted in ( b ) for ASCs and ( f ) for fibroblasts. Global SUMO-2,3 expressions are shown in ( c ) for young and old ASCs, and ( g ) for young and old fibroblasts. Average intensities of the groups are plotted in ( d ) for ASCs and in ( h ) for fibroblasts. Blue color denotes samples from young donors and orange old donors. n = 4 for each ASC group and n = 3 for each fibroblast group. Error bars indicate standard errors. *t-test, 2-tailed, p < 0.05.
Rabbit Anti Human Sumo 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti human sumo 1/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
rabbit anti human sumo 1 - by Bioz Stars, 2025-02
95/100 stars
  Buy from Supplier

Image Search Results


In order to illustrate the preferentially activity of SENP3 to SUMO-1 or SUMO-2/3 mediated SUMOylation profile, quantitative immunoblots of SUMO-1 and its conjugates ( A ) as well as SUMO-2/3 and its conjugates ( B ) were measured in control and SENP3 RNAi GV oocytes. To study the effect of SENP3 RNAi on the localization of SUMO-1 and SUMO-2/3, control or SENP3 RNAi GV oocytes were immunostained for SENP3 and SUMO-1 ( C ) or SUMO-2/3 ( D ). Note that neither SUMO-1 nor SUMO-2/3 localization pattern was altered after SENP3 knockdown. SENP3, red; SUMO-1 and SUMO-2/3, green; DNA, blue. Bar = 10 μm.

Journal: Scientific Reports

Article Title: The SUMO Protease SENP3 Orchestrates G2-M Transition and Spindle Assembly in Mouse Oocytes

doi: 10.1038/srep15600

Figure Lengend Snippet: In order to illustrate the preferentially activity of SENP3 to SUMO-1 or SUMO-2/3 mediated SUMOylation profile, quantitative immunoblots of SUMO-1 and its conjugates ( A ) as well as SUMO-2/3 and its conjugates ( B ) were measured in control and SENP3 RNAi GV oocytes. To study the effect of SENP3 RNAi on the localization of SUMO-1 and SUMO-2/3, control or SENP3 RNAi GV oocytes were immunostained for SENP3 and SUMO-1 ( C ) or SUMO-2/3 ( D ). Note that neither SUMO-1 nor SUMO-2/3 localization pattern was altered after SENP3 knockdown. SENP3, red; SUMO-1 and SUMO-2/3, green; DNA, blue. Bar = 10 μm.

Article Snippet: For immunolabelling, the following primary antibodies and dilutions were used: rabbit anti-SENP3 antibody (1:100), rabbit anti-Bora antibody (Abcam; 1:100), rabbit anti-Aurora A (1:180), rabbit anti-SUMO-1 (1:100), rabbit anti-SUMO-2/3 (1:100), mouse anti-γ-tubulin antibody (Boster, China; 1:50), rabbit anti-Ac-α-tubulin (CST; 1:100), rabbit anti-Cyclin B1 antibody (Affinity; 1:100) or FITC-labelled mouse anti-α-tubulin (1:100).

Techniques: Activity Assay, Western Blot

A) Representative confocal micrographs showing the nuclear and cytoplasmatic expression of human POLR2E and SUMO-1 proteins in vivo, in kidney sections of cisplatin-AKI (AKI-CIS) mice treated or not with MVs and sacrificed 48 hours later, and in vitro by TECs treated with cisplatin and cultured in the absence (vehicle) or in the presence of 50 µg of MVs (MV) for 24 hours. Nuclei were counterstained with Hoechst dye. Original magnification: ×400 for kidney sections and ×630 for TECs. B) 1×10 5 TECs treated with cisplatin and cultured in the absence (CIS) or in the presence of two different preparations of MVs (+MV) for 1 hour were analysed by RT-PCR for specific human mRNA POLR2E . Bands of PCR products specific for human POLR2E of the expected size (90 pb) were detected in a 4% agarose gel electrophoresis. As positive control the extract of human bone marrow-derived MSCs (BM-MSC) was used. The * indicates the control without cDNA.

Journal: PLoS ONE

Article Title: Microvesicles Derived from Mesenchymal Stem Cells Enhance Survival in a Lethal Model of Acute Kidney Injury

doi: 10.1371/journal.pone.0033115

Figure Lengend Snippet: A) Representative confocal micrographs showing the nuclear and cytoplasmatic expression of human POLR2E and SUMO-1 proteins in vivo, in kidney sections of cisplatin-AKI (AKI-CIS) mice treated or not with MVs and sacrificed 48 hours later, and in vitro by TECs treated with cisplatin and cultured in the absence (vehicle) or in the presence of 50 µg of MVs (MV) for 24 hours. Nuclei were counterstained with Hoechst dye. Original magnification: ×400 for kidney sections and ×630 for TECs. B) 1×10 5 TECs treated with cisplatin and cultured in the absence (CIS) or in the presence of two different preparations of MVs (+MV) for 1 hour were analysed by RT-PCR for specific human mRNA POLR2E . Bands of PCR products specific for human POLR2E of the expected size (90 pb) were detected in a 4% agarose gel electrophoresis. As positive control the extract of human bone marrow-derived MSCs (BM-MSC) was used. The * indicates the control without cDNA.

Article Snippet: Section were blocked and labelled with rabbit anti-human POLR2E (Abcam, Cambridge Science Park, UK) (1∶300 dilution) or rabbit anti-human SUMO-1 (Abcam) (1∶300 dilution).

Techniques: Expressing, In Vivo, In Vitro, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, Positive Control, Derivative Assay

A) Representative MV size analyses by direct measurement with NTA, showing no difference among MVs treated or not with RNase. B) Representative Bioanalyzer profile, showing the size distribution of total RNA extracted from MVs treated or not with RNAse. The first peak (left side of each panel) represents an internal standard. The two peaks in Sample 1 (black arrows) represent 18 S (left) and 28 S (right) ribosomal RNA, only partially detectable in MVs. The red arrows showed the reduction of 18 and 28 S fragment inside RNAse-treated MVs. C) Histogram showing the expression level of SUMO-1 , POLR2 and Act B transcripts in MVs treated or not with RNase, express as 2 -δCt , as described in material and methods.

Journal: PLoS ONE

Article Title: Microvesicles Derived from Mesenchymal Stem Cells Enhance Survival in a Lethal Model of Acute Kidney Injury

doi: 10.1371/journal.pone.0033115

Figure Lengend Snippet: A) Representative MV size analyses by direct measurement with NTA, showing no difference among MVs treated or not with RNase. B) Representative Bioanalyzer profile, showing the size distribution of total RNA extracted from MVs treated or not with RNAse. The first peak (left side of each panel) represents an internal standard. The two peaks in Sample 1 (black arrows) represent 18 S (left) and 28 S (right) ribosomal RNA, only partially detectable in MVs. The red arrows showed the reduction of 18 and 28 S fragment inside RNAse-treated MVs. C) Histogram showing the expression level of SUMO-1 , POLR2 and Act B transcripts in MVs treated or not with RNase, express as 2 -δCt , as described in material and methods.

Article Snippet: Section were blocked and labelled with rabbit anti-human POLR2E (Abcam, Cambridge Science Park, UK) (1∶300 dilution) or rabbit anti-human SUMO-1 (Abcam) (1∶300 dilution).

Techniques: Expressing

SUMO-1 and SUMO-2,3 protein expression. Dot blot analysis of global SUMO-1 expressions are shown in ( a ) for young and old ASCs, and ( e ) for young and old fibroblasts. Treatment conditions were: ctrl – at baseline without treatment; HS -heat shock at 42 °C for 1 h and HS/Rec: heat shock at 42 °C for 1 h followed by 1 h recovery at 37 °C. The intensities of the dots were quantified using ImageJ. Average intensities of the groups are plotted in ( b ) for ASCs and ( f ) for fibroblasts. Global SUMO-2,3 expressions are shown in ( c ) for young and old ASCs, and ( g ) for young and old fibroblasts. Average intensities of the groups are plotted in ( d ) for ASCs and in ( h ) for fibroblasts. Blue color denotes samples from young donors and orange old donors. n = 4 for each ASC group and n = 3 for each fibroblast group. Error bars indicate standard errors. *t-test, 2-tailed, p < 0.05.

Journal: Scientific Reports

Article Title: Age Alters Chromatin Structure and Expression of SUMO Proteins under Stress Conditions in Human Adipose-Derived Stem Cells

doi: 10.1038/s41598-018-29775-y

Figure Lengend Snippet: SUMO-1 and SUMO-2,3 protein expression. Dot blot analysis of global SUMO-1 expressions are shown in ( a ) for young and old ASCs, and ( e ) for young and old fibroblasts. Treatment conditions were: ctrl – at baseline without treatment; HS -heat shock at 42 °C for 1 h and HS/Rec: heat shock at 42 °C for 1 h followed by 1 h recovery at 37 °C. The intensities of the dots were quantified using ImageJ. Average intensities of the groups are plotted in ( b ) for ASCs and ( f ) for fibroblasts. Global SUMO-2,3 expressions are shown in ( c ) for young and old ASCs, and ( g ) for young and old fibroblasts. Average intensities of the groups are plotted in ( d ) for ASCs and in ( h ) for fibroblasts. Blue color denotes samples from young donors and orange old donors. n = 4 for each ASC group and n = 3 for each fibroblast group. Error bars indicate standard errors. *t-test, 2-tailed, p < 0.05.

Article Snippet: The primary antibodies used were: rabbit anti-human SUMO-1 and SUMO-2,3 (Cell Signaling Technology, Danvers, MA).

Techniques: Expressing, Dot Blot