Journal: International Journal of Molecular Sciences

Article Title: Anti-Inflammatory Effects of 1,25(OH)2D/Calcitriol in T Cell Immunity: Does Sex Make a Difference?

doi: 10.3390/ijms23169164

Figure Lengend Snippet: Calcitriol upregulates VDR expression in activated peripheral blood T lymphocytes from female and male healthy donors. VDR levels were evaluated by Western blot analysis of T cell lysates after 48 h of calcitriol treatment. ( A ) Blots shown are representative of experiments performed in T cells from 10 randomly selected female and male healthy subjects. ( B ) Densitometry analysis of VDR levels relative to glyceraldehyde 3−phosphate dehydrogenase (GAPDH) is shown. Results are shown as mean ± SD. * p < 0.05 versus untreated cells.

Article Snippet: To ensure the presence of equal amounts of protein, the membranes were re-proved with rabbit anti-human glyceraldehyde 3-phosphate dehydrogenase Ab (GAPDH, Sigma).

Techniques: Expressing, Western Blot