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Santa Cruz Biotechnology rabbit anti human cdc2 506 p34 cdk1
Rabbit Anti Human Cdc2 506 P34 Cdk1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti human cdc2 506 p34 cdk1/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit anti human cdc2 506 p34 cdk1 - by Bioz Stars, 2024-11
86/100 stars

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Santa Cruz Biotechnology rabbit anti human cdc2 506 p34 cdk1
Rabbit Anti Human Cdc2 506 P34 Cdk1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology rabbit anti human cdc2 p34 cdk1
USP18 interacts with SKP2 and SAMHD1 and complexes with cyclin A2, <t>CDK1,</t> and CDK2. (A) Immunoprecipitation of HA-USP18, coexpressed with FLAG-SAMHD1 (lane 2), MYC-SKP2 (lane 3) or all three expression plasmids in a ratio of 1:1:1 (lane 4) using anti-HA affinity matrix beads. In parallel, FLAG-SAMHD1 was coexpressed with MYC-SKP2 and immunoprecipitated with anti-FLAG affinity matrix beads (lanes 7). Single and double transfections were supplemented with pcDNA3.1(+). Cell lysates were immunoblotted for USP18, SKP2, and SAMHD1, using their respective epitopped tags. (B) Immunoprecititation of FLAG-SAMHD1 coexpressed with pLOC-USP18 or without pLOC-USP18 and replaced by pcDNA3.1+. Proteins of HEK293T cells were immunoprecipitated with anti-FLAG affinity matrix beads and immunoblotted with FLAG and USP18 and endogenous expression of cyclin A2 and CDK1, cyclin A2 (B), and CDK2 (C). Each panel is representative of at least three independent experiments.
Rabbit Anti Human Cdc2 P34 Cdk1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti human cdc2 p34 cdk1/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit anti human cdc2 p34 cdk1 - by Bioz Stars, 2024-11
86/100 stars
  Buy from Supplier

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Proteintech rabbit anti human cdc2 p34 cdk1
USP18 interacts with SKP2 and SAMHD1 and complexes with cyclin A2, <t>CDK1,</t> and CDK2. (A) Immunoprecipitation of HA-USP18, coexpressed with FLAG-SAMHD1 (lane 2), MYC-SKP2 (lane 3) or all three expression plasmids in a ratio of 1:1:1 (lane 4) using anti-HA affinity matrix beads. In parallel, FLAG-SAMHD1 was coexpressed with MYC-SKP2 and immunoprecipitated with anti-FLAG affinity matrix beads (lanes 7). Single and double transfections were supplemented with pcDNA3.1(+). Cell lysates were immunoblotted for USP18, SKP2, and SAMHD1, using their respective epitopped tags. (B) Immunoprecititation of FLAG-SAMHD1 coexpressed with pLOC-USP18 or without pLOC-USP18 and replaced by pcDNA3.1+. Proteins of HEK293T cells were immunoprecipitated with anti-FLAG affinity matrix beads and immunoblotted with FLAG and USP18 and endogenous expression of cyclin A2 and CDK1, cyclin A2 (B), and CDK2 (C). Each panel is representative of at least three independent experiments.
Rabbit Anti Human Cdc2 P34 Cdk1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti human cdc2 p34 cdk1/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit anti human cdc2 p34 cdk1 - by Bioz Stars, 2024-11
86/100 stars
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Cell Signaling Technology Inc rabbit anti human cdc2 p34 cdk1
USP18 interacts with SKP2 and SAMHD1 and complexes with cyclin A2, <t>CDK1,</t> and CDK2. (A) Immunoprecipitation of HA-USP18, coexpressed with FLAG-SAMHD1 (lane 2), MYC-SKP2 (lane 3) or all three expression plasmids in a ratio of 1:1:1 (lane 4) using anti-HA affinity matrix beads. In parallel, FLAG-SAMHD1 was coexpressed with MYC-SKP2 and immunoprecipitated with anti-FLAG affinity matrix beads (lanes 7). Single and double transfections were supplemented with pcDNA3.1(+). Cell lysates were immunoblotted for USP18, SKP2, and SAMHD1, using their respective epitopped tags. (B) Immunoprecititation of FLAG-SAMHD1 coexpressed with pLOC-USP18 or without pLOC-USP18 and replaced by pcDNA3.1+. Proteins of HEK293T cells were immunoprecipitated with anti-FLAG affinity matrix beads and immunoblotted with FLAG and USP18 and endogenous expression of cyclin A2 and CDK1, cyclin A2 (B), and CDK2 (C). Each panel is representative of at least three independent experiments.
Rabbit Anti Human Cdc2 P34 Cdk1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti human cdc2 p34 cdk1/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit anti human cdc2 p34 cdk1 - by Bioz Stars, 2024-11
86/100 stars
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Santa Cruz Biotechnology polyclonal rabbit anti human ngf antibody sc548
USP18 interacts with SKP2 and SAMHD1 and complexes with cyclin A2, <t>CDK1,</t> and CDK2. (A) Immunoprecipitation of HA-USP18, coexpressed with FLAG-SAMHD1 (lane 2), MYC-SKP2 (lane 3) or all three expression plasmids in a ratio of 1:1:1 (lane 4) using anti-HA affinity matrix beads. In parallel, FLAG-SAMHD1 was coexpressed with MYC-SKP2 and immunoprecipitated with anti-FLAG affinity matrix beads (lanes 7). Single and double transfections were supplemented with pcDNA3.1(+). Cell lysates were immunoblotted for USP18, SKP2, and SAMHD1, using their respective epitopped tags. (B) Immunoprecititation of FLAG-SAMHD1 coexpressed with pLOC-USP18 or without pLOC-USP18 and replaced by pcDNA3.1+. Proteins of HEK293T cells were immunoprecipitated with anti-FLAG affinity matrix beads and immunoblotted with FLAG and USP18 and endogenous expression of cyclin A2 and CDK1, cyclin A2 (B), and CDK2 (C). Each panel is representative of at least three independent experiments.
Polyclonal Rabbit Anti Human Ngf Antibody Sc548, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


USP18 interacts with SKP2 and SAMHD1 and complexes with cyclin A2, CDK1, and CDK2. (A) Immunoprecipitation of HA-USP18, coexpressed with FLAG-SAMHD1 (lane 2), MYC-SKP2 (lane 3) or all three expression plasmids in a ratio of 1:1:1 (lane 4) using anti-HA affinity matrix beads. In parallel, FLAG-SAMHD1 was coexpressed with MYC-SKP2 and immunoprecipitated with anti-FLAG affinity matrix beads (lanes 7). Single and double transfections were supplemented with pcDNA3.1(+). Cell lysates were immunoblotted for USP18, SKP2, and SAMHD1, using their respective epitopped tags. (B) Immunoprecititation of FLAG-SAMHD1 coexpressed with pLOC-USP18 or without pLOC-USP18 and replaced by pcDNA3.1+. Proteins of HEK293T cells were immunoprecipitated with anti-FLAG affinity matrix beads and immunoblotted with FLAG and USP18 and endogenous expression of cyclin A2 and CDK1, cyclin A2 (B), and CDK2 (C). Each panel is representative of at least three independent experiments.

Journal: Journal of Virology

Article Title: USP18 (UBP43) Abrogates p21-Mediated Inhibition of HIV-1

doi: 10.1128/JVI.00592-18

Figure Lengend Snippet: USP18 interacts with SKP2 and SAMHD1 and complexes with cyclin A2, CDK1, and CDK2. (A) Immunoprecipitation of HA-USP18, coexpressed with FLAG-SAMHD1 (lane 2), MYC-SKP2 (lane 3) or all three expression plasmids in a ratio of 1:1:1 (lane 4) using anti-HA affinity matrix beads. In parallel, FLAG-SAMHD1 was coexpressed with MYC-SKP2 and immunoprecipitated with anti-FLAG affinity matrix beads (lanes 7). Single and double transfections were supplemented with pcDNA3.1(+). Cell lysates were immunoblotted for USP18, SKP2, and SAMHD1, using their respective epitopped tags. (B) Immunoprecititation of FLAG-SAMHD1 coexpressed with pLOC-USP18 or without pLOC-USP18 and replaced by pcDNA3.1+. Proteins of HEK293T cells were immunoprecipitated with anti-FLAG affinity matrix beads and immunoblotted with FLAG and USP18 and endogenous expression of cyclin A2 and CDK1, cyclin A2 (B), and CDK2 (C). Each panel is representative of at least three independent experiments.

Article Snippet: In other experiments, endogenous cyclin A2, CDK1, and CDK2 were detected using rabbit anti-human cyclin A2 (18202-1-AP; Proteintech; 1:500 dilution), rabbit anti-human Cdc2 p34/CDK1 (H-297, sc-747; Santa Cruz; 1:500), and rabbit anti-human CDK2 (78B2, 2546; Cell Signaling; 1:500), respectively, after immunoprecipitation of overexpressed SAMHD1-FLAG cotransfected with USP18.

Techniques: Immunoprecipitation, Expressing, Transfection

USP18 interacts with SKP2 and SAMHD1 and complexes with cyclin A2, CDK1, and CDK2. (A) Immunoprecipitation of HA-USP18, coexpressed with FLAG-SAMHD1 (lane 2), MYC-SKP2 (lane 3) or all three expression plasmids in a ratio of 1:1:1 (lane 4) using anti-HA affinity matrix beads. In parallel, FLAG-SAMHD1 was coexpressed with MYC-SKP2 and immunoprecipitated with anti-FLAG affinity matrix beads (lanes 7). Single and double transfections were supplemented with pcDNA3.1(+). Cell lysates were immunoblotted for USP18, SKP2, and SAMHD1, using their respective epitopped tags. (B) Immunoprecititation of FLAG-SAMHD1 coexpressed with pLOC-USP18 or without pLOC-USP18 and replaced by pcDNA3.1+. Proteins of HEK293T cells were immunoprecipitated with anti-FLAG affinity matrix beads and immunoblotted with FLAG and USP18 and endogenous expression of cyclin A2 and CDK1, cyclin A2 (B), and CDK2 (C). Each panel is representative of at least three independent experiments.

Journal: Journal of Virology

Article Title: USP18 (UBP43) Abrogates p21-Mediated Inhibition of HIV-1

doi: 10.1128/JVI.00592-18

Figure Lengend Snippet: USP18 interacts with SKP2 and SAMHD1 and complexes with cyclin A2, CDK1, and CDK2. (A) Immunoprecipitation of HA-USP18, coexpressed with FLAG-SAMHD1 (lane 2), MYC-SKP2 (lane 3) or all three expression plasmids in a ratio of 1:1:1 (lane 4) using anti-HA affinity matrix beads. In parallel, FLAG-SAMHD1 was coexpressed with MYC-SKP2 and immunoprecipitated with anti-FLAG affinity matrix beads (lanes 7). Single and double transfections were supplemented with pcDNA3.1(+). Cell lysates were immunoblotted for USP18, SKP2, and SAMHD1, using their respective epitopped tags. (B) Immunoprecititation of FLAG-SAMHD1 coexpressed with pLOC-USP18 or without pLOC-USP18 and replaced by pcDNA3.1+. Proteins of HEK293T cells were immunoprecipitated with anti-FLAG affinity matrix beads and immunoblotted with FLAG and USP18 and endogenous expression of cyclin A2 and CDK1, cyclin A2 (B), and CDK2 (C). Each panel is representative of at least three independent experiments.

Article Snippet: In other experiments, endogenous cyclin A2, CDK1, and CDK2 were detected using rabbit anti-human cyclin A2 (18202-1-AP; Proteintech; 1:500 dilution), rabbit anti-human Cdc2 p34/CDK1 (H-297, sc-747; Santa Cruz; 1:500), and rabbit anti-human CDK2 (78B2, 2546; Cell Signaling; 1:500), respectively, after immunoprecipitation of overexpressed SAMHD1-FLAG cotransfected with USP18.

Techniques: Immunoprecipitation, Expressing, Transfection

USP18 interacts with SKP2 and SAMHD1 and complexes with cyclin A2, CDK1, and CDK2. (A) Immunoprecipitation of HA-USP18, coexpressed with FLAG-SAMHD1 (lane 2), MYC-SKP2 (lane 3) or all three expression plasmids in a ratio of 1:1:1 (lane 4) using anti-HA affinity matrix beads. In parallel, FLAG-SAMHD1 was coexpressed with MYC-SKP2 and immunoprecipitated with anti-FLAG affinity matrix beads (lanes 7). Single and double transfections were supplemented with pcDNA3.1(+). Cell lysates were immunoblotted for USP18, SKP2, and SAMHD1, using their respective epitopped tags. (B) Immunoprecititation of FLAG-SAMHD1 coexpressed with pLOC-USP18 or without pLOC-USP18 and replaced by pcDNA3.1+. Proteins of HEK293T cells were immunoprecipitated with anti-FLAG affinity matrix beads and immunoblotted with FLAG and USP18 and endogenous expression of cyclin A2 and CDK1, cyclin A2 (B), and CDK2 (C). Each panel is representative of at least three independent experiments.

Journal: Journal of Virology

Article Title: USP18 (UBP43) Abrogates p21-Mediated Inhibition of HIV-1

doi: 10.1128/JVI.00592-18

Figure Lengend Snippet: USP18 interacts with SKP2 and SAMHD1 and complexes with cyclin A2, CDK1, and CDK2. (A) Immunoprecipitation of HA-USP18, coexpressed with FLAG-SAMHD1 (lane 2), MYC-SKP2 (lane 3) or all three expression plasmids in a ratio of 1:1:1 (lane 4) using anti-HA affinity matrix beads. In parallel, FLAG-SAMHD1 was coexpressed with MYC-SKP2 and immunoprecipitated with anti-FLAG affinity matrix beads (lanes 7). Single and double transfections were supplemented with pcDNA3.1(+). Cell lysates were immunoblotted for USP18, SKP2, and SAMHD1, using their respective epitopped tags. (B) Immunoprecititation of FLAG-SAMHD1 coexpressed with pLOC-USP18 or without pLOC-USP18 and replaced by pcDNA3.1+. Proteins of HEK293T cells were immunoprecipitated with anti-FLAG affinity matrix beads and immunoblotted with FLAG and USP18 and endogenous expression of cyclin A2 and CDK1, cyclin A2 (B), and CDK2 (C). Each panel is representative of at least three independent experiments.

Article Snippet: In other experiments, endogenous cyclin A2, CDK1, and CDK2 were detected using rabbit anti-human cyclin A2 (18202-1-AP; Proteintech; 1:500 dilution), rabbit anti-human Cdc2 p34/CDK1 (H-297, sc-747; Santa Cruz; 1:500), and rabbit anti-human CDK2 (78B2, 2546; Cell Signaling; 1:500), respectively, after immunoprecipitation of overexpressed SAMHD1-FLAG cotransfected with USP18.

Techniques: Immunoprecipitation, Expressing, Transfection