Journal: Journal of Healthcare Engineering

Article Title: Effect and Mechanism of Lidocaine Pretreatment Combined with Dexmedetomidine on Oxidative Stress in Patients with Intracranial Aneurysm Clipping

doi: 10.1155/2021/4293900

Figure Lengend Snippet: Western blotting results. (a) Western blotting is used to detect the protein expressions of nNOS, iNOS, and p22phox, p91phox, and p47phox in rat brain tissues; (b) histogram of the relative protein gray values in (a). There are significant differences between groups, ∗ P < 0.05, ∗∗ P < 0.01.

Article Snippet: Rabbit anti-human polyclonal antibodies p22phox (item no. 66020-1-lg), p47phox (item number 28187-1-AP), horseradish peroxidase-labeled goat anti-rabbit IgG, and horseradish peroxidase-labeled goat anti-mouse IgG were purchased from Proteintech.

Techniques: Western Blot

Journal: ACS nano

Article Title: Nanoparticle-Mediated Co-Delivery of Notch-1 Antibodies and ABT-737 as a Potent Treatment Strategy for Triple-Negative Breast Cancer

doi: 10.1021/acsnano.9b09263

Figure Lengend Snippet: a(a) Notch signaling is activated in TNBC cells when overexpressed Notch-1 receptors interact with Jagged or Delta ligands on neighboring cells. This leads to cleavage of the NICD, which translocates to the nucleus to activate signaling that supports TNBC cell survival, proliferation, and apoptosis suppression. Bcl-2 is an anti-apoptotic protein that is also overexpressed in TNBC and contributes to poor clinical outcomes. (b) Scheme depicting the posited effects of N1-ABT-NPs on TNBC cells. N1-ABT-NPs can bind Notch-1 receptors on TNBC cells to lock them in a ligand unresponsive state, thereby turning off Notch signaling. Simultaneously, these NPs can release the Bcl-2 inhibitor ABT-737. Together, these effects promote apoptosis and reduce cellular proliferation. This figure was produced with permission using Servier Medical ART templates, which are licensed under a Creative Commons Attribution 3.0 Unported License from Servier Medical Art; https://smart.servier.com.

Article Snippet: The membrane was subsequently blocked for 60 min in 5% milk in tris buffered saline with 0.1% Tween-20 (TBS-T) and then incubated with rabbit anti-human Bcl-2 (ProteinTech; 1:1000), cleaved Notch-1 (Cell Signaling Technology; 1:250), and Hes1 antibodies (Cell Signaling Technology; 1:500) in 5% milk in TBS-T overnight at 4 °C.

Techniques: Produced

Journal: ACS nano

Article Title: Nanoparticle-Mediated Co-Delivery of Notch-1 Antibodies and ABT-737 as a Potent Treatment Strategy for Triple-Negative Breast Cancer

doi: 10.1021/acsnano.9b09263

Figure Lengend Snippet: Examination of the effects of N1-ABT-NPs on Notch signaling and Bcl-2 expression in TNBC cells. (a) Scheme of proposed NP interaction with MDA-MB-231 TNBC cells. Upon cellular binding, N1-ABT-NPs suppress Notch signaling through antibody-mediated signal cascade interference and also release ABT-737 to inhibit Bcl-2 and activate apoptosis. (b) qRT-PCR analysis of relative Bcl-2, Noxa, Hes5, and HeyL mRNA expression after treatment with IgG- or N1-ABT-NPs compared to control (untreated) cells (n = 3). RPLPO was used as a control and relative mRNA expression in NP treated groups is normalized to that of untreated cells. *p < 0.05, ##p = 0.06, #p = 0.07 (c) Quazi-quantitative analysis of Western blotting for normalized Bcl-2, cleaved Notch-1, and Hes1 protein expression (n = 3). β-actin was used as a control, and expression in NP-treated samples was normalized to expression in untreated cells. **p < 0.01. (d) Representative Western blot bands for Bcl-2, cleaved Notch-1, Hes1, and β-actin protein levels. Portions of this figure were produced with permission using Servier Medical ART templates, which are licensed under a Creative Commons Attribution 3.0 Unported License from Servier Medical Art; https://smart.servier.com.

Article Snippet: The membrane was subsequently blocked for 60 min in 5% milk in tris buffered saline with 0.1% Tween-20 (TBS-T) and then incubated with rabbit anti-human Bcl-2 (ProteinTech; 1:1000), cleaved Notch-1 (Cell Signaling Technology; 1:250), and Hes1 antibodies (Cell Signaling Technology; 1:500) in 5% milk in TBS-T overnight at 4 °C.

Techniques: Expressing, Binding Assay, Quantitative RT-PCR, Western Blot, Produced