Journal: Cell Biology and Toxicology
Article Title: E3 ubiquitin ligase trim36 targets ddx3x for degradation to reprogram macrophage polarization and ameliorate tubal factor infertility in rats
doi: 10.1007/s10565-026-10140-z
Figure Lengend Snippet: Silence of DDX3X in rat BMDM inhibited inflammation in the LPS-induced fallopian tube epithelial cells. ( A ) Cell morphology of fallopian tube epithelial cells was observed under a microscope. ( B ) IF detection of cytokeratin 18 and Vimentin in fallopian tube epithelial cells. ( C ) CCK8 assay of cell viability. ( D ) TUNEL staining of cell apoptosis. ( E ) Transwell assay of cell migration. ( F ) RT-qPCR detection of TNF-α, IL-1β, and IL-10 levels. ( G ) ELISA detection of IL-1β, TNF-α, and IL-10 levels. *P < 0.05, **P < 0.01, ***P < 0.001. n = 3 biologically independent experiments. P values in results (C-G) were determined by two-way ANOVA
Article Snippet: The incubation of primary antibody was executed at 4 °C overnight through two antibodies: a rabbit polyclonal antibody against Cytokeratin 18 (Catalog #10,830–1-AP, Proteintech, USA) diluted with 1:100, and a rabbit polyclonal antibody against Vimentin (Catalog #10,366–1-AP, Proteintech, USA) diluted with 1:50.
Techniques: Microscopy, CCK-8 Assay, TUNEL Assay, Staining, Transwell Assay, Migration, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay