rabbit cav1 2  (Alomone Labs)


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    Alomone Labs rabbit cav1 2
    Rabbit Cav1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit cav1 2/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit cav1 2 - by Bioz Stars, 2022-11
    95/100 stars

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    Alomone Labs anti cav1 2 cacna1c antibody
    <t>Cav1.2</t> maintained Wnt/β-catenin signaling activity in AM tumoroids. A – C Bright field images of an AM tumoroid generated from a single cell. D The organoid forming efficiency was evaluated as a percentage of the number of tumoroids in each well. The <t>CACNA1C</t> overexpression effectively retained the organoid forming efficiency compared to vehicle and VPM treatment group. E – G Hematoxylin and eosin staining of paraffin sections of AM tumoroids. Spherical shaped AM tumoroids were observed in vehicle and VPM treatment group. Budding-like structures expended from CACNA1C-overexpressed AM tumoroids. H Quantification of the size of 14 days and 21 days cultured AM tumoroids. I – K Immunohistochemistry staining of β-catenin in an AM tumoroid. β-catenin was primary found in the plasma membrane. The nuclear accumulation of β-catenin was dominantly observed in the overexpression group compared to the vehicle and VPM treatment groups. L – M Immunohistochemistry staining of CK10 and CACNA1C. The white rectangle indicates the CK10 positive differentiated AM cells in the vehicle and VPM treatment groups. Nuclei were counterstained by TO-PRO-3 (TP3). O Western blot assay of nucleus and cytoplasm fractionated AM tumoroids with β-catenin <t>antibody.</t> P The relative mRNA expression of WNT3A, AXIN2, CTNNB1 , and LGR5 in CACNA1C-overexpressed and VPM treated tumoroids. Scale bar: A–C, 300 μm; E–G, I–K, L–M, 100 μm. Data are presented as the mean ± SD of triplicate experiments. * p
    Anti Cav1 2 Cacna1c Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cav1 2 cacna1c antibody/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cav1 2 cacna1c antibody - by Bioz Stars, 2022-11
    95/100 stars
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    Cav1.2 maintained Wnt/β-catenin signaling activity in AM tumoroids. A – C Bright field images of an AM tumoroid generated from a single cell. D The organoid forming efficiency was evaluated as a percentage of the number of tumoroids in each well. The CACNA1C overexpression effectively retained the organoid forming efficiency compared to vehicle and VPM treatment group. E – G Hematoxylin and eosin staining of paraffin sections of AM tumoroids. Spherical shaped AM tumoroids were observed in vehicle and VPM treatment group. Budding-like structures expended from CACNA1C-overexpressed AM tumoroids. H Quantification of the size of 14 days and 21 days cultured AM tumoroids. I – K Immunohistochemistry staining of β-catenin in an AM tumoroid. β-catenin was primary found in the plasma membrane. The nuclear accumulation of β-catenin was dominantly observed in the overexpression group compared to the vehicle and VPM treatment groups. L – M Immunohistochemistry staining of CK10 and CACNA1C. The white rectangle indicates the CK10 positive differentiated AM cells in the vehicle and VPM treatment groups. Nuclei were counterstained by TO-PRO-3 (TP3). O Western blot assay of nucleus and cytoplasm fractionated AM tumoroids with β-catenin antibody. P The relative mRNA expression of WNT3A, AXIN2, CTNNB1 , and LGR5 in CACNA1C-overexpressed and VPM treated tumoroids. Scale bar: A–C, 300 μm; E–G, I–K, L–M, 100 μm. Data are presented as the mean ± SD of triplicate experiments. * p

    Journal: Cell & Bioscience

    Article Title: Unraveled roles of Cav1.2 in proliferation and stemness of ameloblastoma

    doi: 10.1186/s13578-022-00873-9

    Figure Lengend Snippet: Cav1.2 maintained Wnt/β-catenin signaling activity in AM tumoroids. A – C Bright field images of an AM tumoroid generated from a single cell. D The organoid forming efficiency was evaluated as a percentage of the number of tumoroids in each well. The CACNA1C overexpression effectively retained the organoid forming efficiency compared to vehicle and VPM treatment group. E – G Hematoxylin and eosin staining of paraffin sections of AM tumoroids. Spherical shaped AM tumoroids were observed in vehicle and VPM treatment group. Budding-like structures expended from CACNA1C-overexpressed AM tumoroids. H Quantification of the size of 14 days and 21 days cultured AM tumoroids. I – K Immunohistochemistry staining of β-catenin in an AM tumoroid. β-catenin was primary found in the plasma membrane. The nuclear accumulation of β-catenin was dominantly observed in the overexpression group compared to the vehicle and VPM treatment groups. L – M Immunohistochemistry staining of CK10 and CACNA1C. The white rectangle indicates the CK10 positive differentiated AM cells in the vehicle and VPM treatment groups. Nuclei were counterstained by TO-PRO-3 (TP3). O Western blot assay of nucleus and cytoplasm fractionated AM tumoroids with β-catenin antibody. P The relative mRNA expression of WNT3A, AXIN2, CTNNB1 , and LGR5 in CACNA1C-overexpressed and VPM treated tumoroids. Scale bar: A–C, 300 μm; E–G, I–K, L–M, 100 μm. Data are presented as the mean ± SD of triplicate experiments. * p

    Article Snippet: Rabbit anti-CACNA1C (1:200, Alomone Labs, ACC-003), mouse anti-CK14 (1:500, Abcam, ab7800), mouse anti-CK10 (1:500, Invitrogen, MA5-13705), mouse anti-E-cadherin (1:500, BD Biosciences, AF748), rabbit anti-MMP-9 (1:200, Merck, AB19016), rabbit anti-LGR5 (1:200, Abcam, ab75732), mouse anti-Ki67 (1:200, Abcam, ab16667), mouse anti-PCNA (1:500, Abcam, ab29), mouse anti-NFATc1 (1:200, Santa Cruz, SC-7294), and mouse anti-β-catenin (1:500, Santa Cruz, SC-7963).

    Techniques: Activity Assay, Generated, Over Expression, Staining, Cell Culture, Immunohistochemistry, Western Blot, Expressing

    Cav1.2-dependent Ca 2+ /NFATc1 signaling increased the proliferation of AM cells. A – D Representative CACNA1C expression in AM cells indicated by arrowhead. CACNA1C was intensely expressed in the plasma membrane in the vehicle group. And it was broadly expressed in the plasma membrane, cytoplasm and nucleus in overexpression group. CACNA1C negatively expressed in knockdown group compared to the scramble. E – H The nuclear translocation of NFATc1 was observed in the overexpression group compared to the vehicle. NFATc1 was negatively expressed in the knockdown group compared to the scramble. I – L Representative confocal images of Ki67 staining of AM cells among the vehicle, overexpression, scramble, and siRNA groups. Nuclear were stained with TO-PRO-3 (TP3). M Percentages of CACNA1C + and NFATc1 + cells were quantified from immunostained images (N = 5 per group, biological replication). N Relative mRNA expression of CACNA1C , MKI67 , and NFATC1 between vehicle and CACNA1C overexpression, or scramble and siRNA group. O Western blot assay of AM cells with CACNA1C, PCNA, Cyclin D1, and GAPDH antibodies. P Western blot assay of nucleus and cytoplasm fractionated AM cells with NFATc1, Histone H3, and GAPDH antibodies. Scale bar: A–D, 50 μm; E–L, 100 μm. Quantitative data are presented as the mean ± SD. ** p

    Journal: Cell & Bioscience

    Article Title: Unraveled roles of Cav1.2 in proliferation and stemness of ameloblastoma

    doi: 10.1186/s13578-022-00873-9

    Figure Lengend Snippet: Cav1.2-dependent Ca 2+ /NFATc1 signaling increased the proliferation of AM cells. A – D Representative CACNA1C expression in AM cells indicated by arrowhead. CACNA1C was intensely expressed in the plasma membrane in the vehicle group. And it was broadly expressed in the plasma membrane, cytoplasm and nucleus in overexpression group. CACNA1C negatively expressed in knockdown group compared to the scramble. E – H The nuclear translocation of NFATc1 was observed in the overexpression group compared to the vehicle. NFATc1 was negatively expressed in the knockdown group compared to the scramble. I – L Representative confocal images of Ki67 staining of AM cells among the vehicle, overexpression, scramble, and siRNA groups. Nuclear were stained with TO-PRO-3 (TP3). M Percentages of CACNA1C + and NFATc1 + cells were quantified from immunostained images (N = 5 per group, biological replication). N Relative mRNA expression of CACNA1C , MKI67 , and NFATC1 between vehicle and CACNA1C overexpression, or scramble and siRNA group. O Western blot assay of AM cells with CACNA1C, PCNA, Cyclin D1, and GAPDH antibodies. P Western blot assay of nucleus and cytoplasm fractionated AM cells with NFATc1, Histone H3, and GAPDH antibodies. Scale bar: A–D, 50 μm; E–L, 100 μm. Quantitative data are presented as the mean ± SD. ** p

    Article Snippet: Rabbit anti-CACNA1C (1:200, Alomone Labs, ACC-003), mouse anti-CK14 (1:500, Abcam, ab7800), mouse anti-CK10 (1:500, Invitrogen, MA5-13705), mouse anti-E-cadherin (1:500, BD Biosciences, AF748), rabbit anti-MMP-9 (1:200, Merck, AB19016), rabbit anti-LGR5 (1:200, Abcam, ab75732), mouse anti-Ki67 (1:200, Abcam, ab16667), mouse anti-PCNA (1:500, Abcam, ab29), mouse anti-NFATc1 (1:200, Santa Cruz, SC-7294), and mouse anti-β-catenin (1:500, Santa Cruz, SC-7963).

    Techniques: Expressing, Over Expression, Translocation Assay, Staining, Western Blot

    Schematic summary. Cav1.2 is dominantly expressed in ameloblastoma cells compared to the OKC. The Cav1.2-dependent Ca 2+ influx increases the proliferation of ameloblastoma cells by the accumulation of nucleic NFATc1, while intracellular Ca 2+ enhances Wnt/β-catenin signaling activity and the maintenance of the stemness of the ameloblastoma cells

    Journal: Cell & Bioscience

    Article Title: Unraveled roles of Cav1.2 in proliferation and stemness of ameloblastoma

    doi: 10.1186/s13578-022-00873-9

    Figure Lengend Snippet: Schematic summary. Cav1.2 is dominantly expressed in ameloblastoma cells compared to the OKC. The Cav1.2-dependent Ca 2+ influx increases the proliferation of ameloblastoma cells by the accumulation of nucleic NFATc1, while intracellular Ca 2+ enhances Wnt/β-catenin signaling activity and the maintenance of the stemness of the ameloblastoma cells

    Article Snippet: Rabbit anti-CACNA1C (1:200, Alomone Labs, ACC-003), mouse anti-CK14 (1:500, Abcam, ab7800), mouse anti-CK10 (1:500, Invitrogen, MA5-13705), mouse anti-E-cadherin (1:500, BD Biosciences, AF748), rabbit anti-MMP-9 (1:200, Merck, AB19016), rabbit anti-LGR5 (1:200, Abcam, ab75732), mouse anti-Ki67 (1:200, Abcam, ab16667), mouse anti-PCNA (1:500, Abcam, ab29), mouse anti-NFATc1 (1:200, Santa Cruz, SC-7294), and mouse anti-β-catenin (1:500, Santa Cruz, SC-7963).

    Techniques: Activity Assay

    Protein expression patterns of Cav1.2 in patient tissue samples without and with epipharyngeal abrasive therapy (EAT). (A) Cav1.2 expression in the epipharynx of the EAT-treated and non-treated groups. (B) Immunohistochemical (IHC) scores for Cav1.2 expression on the epipharyngeal mucosa of the EAT-treated (n = 11) and non-treated groups (n = 7). **Significantly different at p

    Journal: In Vivo

    Article Title: Epipharyngeal Abrasive Therapy Down-regulates the Expression of Cav1.2: A Key Molecule in Influenza Virus Entry

    doi: 10.21873/invivo.12967

    Figure Lengend Snippet: Protein expression patterns of Cav1.2 in patient tissue samples without and with epipharyngeal abrasive therapy (EAT). (A) Cav1.2 expression in the epipharynx of the EAT-treated and non-treated groups. (B) Immunohistochemical (IHC) scores for Cav1.2 expression on the epipharyngeal mucosa of the EAT-treated (n = 11) and non-treated groups (n = 7). **Significantly different at p

    Article Snippet: Rabbit antibody to CaV1.2 (#ACC-003) was purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Expressing, Immunohistochemistry