rabbit polyclonal anti aldh1a1  (Millipore)

Journal: iScience

Article Title: Alphaherpesvirus manipulates retinoic acid metabolism for optimal replication

doi: 10.1016/j.isci.2024.110144

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-ALDH1A1 , Proteintech , Cat# 15910-1-AP RRID: AB_2305276.

Techniques: Recombinant, Transfection, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Software

Journal: PLoS ONE

Article Title: Transcriptional Landscape of Glomerular Parietal Epithelial Cells

doi: 10.1371/journal.pone.0105289

Figure Lengend Snippet: Agarose gels were loaded as follows: lane 1 = DNA size marker, lane 2 = mPEC, lane 3 = mPEC no primer control, lane 4 = kidney cortex (positive control), lane 5 = kidney cortex no primer control. Arrows indicate positive bands: (A) Hnf1b ; (B) Aldh1a1 ; (C) Cdh6 ; (D) Cdh11 ; (E) Cdkl1 ; (F) Fras1 ; (G) Pax8 ; (H) Tfcp2l1 ; (I) Clmn ; (J) Prelp ; (K) Lad1 ; (L) Wwc1 .

Article Snippet: The following antibodies were used: rabbit polyclonal anti-CDH6 1∶5000 (Abgent, San Diego, CA), rabbit polyclonal anti-PAX8 1∶250 (ProteinTech Group, Chicago, IL) rabbit polyclonal anti-ALDH1A1 1∶1000 (Epitomics, Burlingame, CA), and rabbit polyclonal anti-FRAS1 1∶2000 (Sigma-Aldrich).

Techniques: Marker, Positive Control

Journal: PLoS ONE

Article Title: Transcriptional Landscape of Glomerular Parietal Epithelial Cells

doi: 10.1371/journal.pone.0105289

Figure Lengend Snippet: Panels A–D show representative images of immunohistochemistry on rat kidneys for: (A) FRAS1, cytoplasmic staining (brown) was present within PECs (thin arrows) as well as proximal tubules (thick arrow); (B) ALDH1A1, cytoplasmic staining (brown) was present within PECs (thin arrows) as well as proximal tubules and collecting duct (thick arrows); (C) CDH6, membrane staining (brown) was present in PECs (thin arrows) as well as the brush borders of proximal tubules (thick arrow); (D) PAX8, nuclear staining (brown) was present within PECs (thin arrows) as well as some tubules; (E) representative image of negative control with no immunostaining.

Article Snippet: The following antibodies were used: rabbit polyclonal anti-CDH6 1∶5000 (Abgent, San Diego, CA), rabbit polyclonal anti-PAX8 1∶250 (ProteinTech Group, Chicago, IL) rabbit polyclonal anti-ALDH1A1 1∶1000 (Epitomics, Burlingame, CA), and rabbit polyclonal anti-FRAS1 1∶2000 (Sigma-Aldrich).

Techniques: Immunohistochemistry, Staining, Negative Control, Immunostaining

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Expression of Aldehyde Dehydrogenase 1A1 (ALDH1A1) as a Prognostic Biomarker in Colorectal Cancer Using Immunohistochemistry

doi: 10.12659/MSM.910109

Figure Lengend Snippet: Photomicrographs showing the immunohistochemical expression of the aldehyde dehydrogenase 1A1 (ALDH1A1) protein in colorectal cancer (CRC) tissues and non-tumor adjacent tissue (NAT). ( A ) Negative immunostaining for aldehyde dehydrogenase 1A1 (ALDH1A1) protein expression is shown in normal colorectal tissue, or non-tumor adjacent tissue (NAT). (Magnification ×200). ( B ) Negative immunostaining for ALDH1A1 protein expression is shown in normal colorectal tissue or NAT. (Magnification ×400). ( C ) Weak immunostaining for ALDH1A1 protein expression is shown in colorectal carcinoma (CRC) tissue. (Magnification ×200). ( D ) Weak immunostaining for ALDH1A1 protein expression is shown in CRC tissue. (Magnification ×400). ( E ) Moderate immunostaining for ALDH1A1 protein expression is shown in CRC tissue. (Magnification ×200). ( F ) Moderate immunostaining for ALDH1A1 protein expression is shown in CRC tissue. (Magnification ×400). ( G ) Strong immunostaining for ALDH1A1 protein expression is shown in CRC tissue. (Magnification ×200). ( H ) Strong immunostaining for ALDH1A1 protein expression is shown in CRC tissue. (Magnification ×400).

Article Snippet: The primary rabbit anti-ALDH1A1 polyclonal antibody (clone No. ab52492) (Abcam, Cambridge MA, USA) was used at a dilution of 1: 400 in phosphate-buffered saline (PBS).

Techniques: Immunohistochemical staining, Expressing, Immunostaining

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Expression of Aldehyde Dehydrogenase 1A1 (ALDH1A1) as a Prognostic Biomarker in Colorectal Cancer Using Immunohistochemistry

doi: 10.12659/MSM.910109

Figure Lengend Snippet: The expression of of aldehyde dehydrogenase 1A1 (ALDH1A1) in colorectal carcinoma (CRC) tissue compared with non-tumor adjacent tissue (NAT), using immunohistochemistry (IHC).

Article Snippet: The primary rabbit anti-ALDH1A1 polyclonal antibody (clone No. ab52492) (Abcam, Cambridge MA, USA) was used at a dilution of 1: 400 in phosphate-buffered saline (PBS).

Techniques: Expressing, Immunohistochemistry

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Expression of Aldehyde Dehydrogenase 1A1 (ALDH1A1) as a Prognostic Biomarker in Colorectal Cancer Using Immunohistochemistry

doi: 10.12659/MSM.910109

Figure Lengend Snippet: Correlation between clinicopathologic variables and expression of aldehyde dehydrogenase 1A1 (ALDH1A1) protein in colorectal carcinoma (CRC) tumor tissue using immunohistochemistry (IHC).

Article Snippet: The primary rabbit anti-ALDH1A1 polyclonal antibody (clone No. ab52492) (Abcam, Cambridge MA, USA) was used at a dilution of 1: 400 in phosphate-buffered saline (PBS).

Techniques: Expressing, Immunohistochemistry

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Expression of Aldehyde Dehydrogenase 1A1 (ALDH1A1) as a Prognostic Biomarker in Colorectal Cancer Using Immunohistochemistry

doi: 10.12659/MSM.910109

Figure Lengend Snippet: Kaplan-Meier overall survival (OS) curves, stratified according to the status of aldehyde dehydrogenase 1A1 (ALDH1A1) protein expression.

Article Snippet: The primary rabbit anti-ALDH1A1 polyclonal antibody (clone No. ab52492) (Abcam, Cambridge MA, USA) was used at a dilution of 1: 400 in phosphate-buffered saline (PBS).

Techniques: Expressing

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Expression of Aldehyde Dehydrogenase 1A1 (ALDH1A1) as a Prognostic Biomarker in Colorectal Cancer Using Immunohistochemistry

doi: 10.12659/MSM.910109

Figure Lengend Snippet: Univariate and multivariate analysis of prognostic variables in patients with colorectal carcinoma (CRC).

Article Snippet: The primary rabbit anti-ALDH1A1 polyclonal antibody (clone No. ab52492) (Abcam, Cambridge MA, USA) was used at a dilution of 1: 400 in phosphate-buffered saline (PBS).

Techniques:

Journal: Frontiers in Oncology

Article Title: Integrated Analysis of Transcriptome Data Revealed AURKA and KIF20A as Critical Genes in Medulloblastoma Progression

doi: 10.3389/fonc.2022.875521

Figure Lengend Snippet: Functional roles of 12 hub genes.

Article Snippet: AURKA mouse monoclonal antibody (1:400, Proteintech, 66757-1-IG) and KIF20A rabbit polyclonal antibody (1:400, Proteintech, 15910-1-AP) were stained overnight at 4°C, and the second antibody was subjected to incubation for 1 hour at ambient temperature.

Techniques: Functional Assay, DNA Synthesis, Activation Assay

Journal: Frontiers in Oncology

Article Title: Integrated Analysis of Transcriptome Data Revealed AURKA and KIF20A as Critical Genes in Medulloblastoma Progression

doi: 10.3389/fonc.2022.875521

Figure Lengend Snippet: Survival analysis of the 12 hub genes in MED based on the GSE30074 database. (A) AURKA, (B) BUB1B, (C) CCNB1, (D) CCNB2, (E) CHEK1, (F) KIF11, (G) KIF20A, (H) MAD2L1, (I) MCM6, (J) NCAPG, (K) RFC4, (L) RRM2; P < 0.05 was considered statistically significant.

Article Snippet: AURKA mouse monoclonal antibody (1:400, Proteintech, 66757-1-IG) and KIF20A rabbit polyclonal antibody (1:400, Proteintech, 15910-1-AP) were stained overnight at 4°C, and the second antibody was subjected to incubation for 1 hour at ambient temperature.

Techniques:

Journal: Frontiers in Oncology

Article Title: Integrated Analysis of Transcriptome Data Revealed AURKA and KIF20A as Critical Genes in Medulloblastoma Progression

doi: 10.3389/fonc.2022.875521

Figure Lengend Snippet: Survival analysis of AURKA and KIF20A based on the GSE85217 database. (A) AURKA, (B) KIF20A. P < 0.05 was considered statistically significant.

Article Snippet: AURKA mouse monoclonal antibody (1:400, Proteintech, 66757-1-IG) and KIF20A rabbit polyclonal antibody (1:400, Proteintech, 15910-1-AP) were stained overnight at 4°C, and the second antibody was subjected to incubation for 1 hour at ambient temperature.

Techniques:

Journal: Frontiers in Oncology

Article Title: Integrated Analysis of Transcriptome Data Revealed AURKA and KIF20A as Critical Genes in Medulloblastoma Progression

doi: 10.3389/fonc.2022.875521

Figure Lengend Snippet: The different expressions of AURKA and KIF20A in the three databases. (A) Expressions of AURKA in GSE39182, (B) Expressions of AURKA in GSE74195, (C) Expressions of AURKA in GSE86574, (D) Expressions of KIF20A in GSE39182, (E) Expressions of KIF20A in GSE74195, (F) Expressions of KIF20A in GSE86574.

Article Snippet: AURKA mouse monoclonal antibody (1:400, Proteintech, 66757-1-IG) and KIF20A rabbit polyclonal antibody (1:400, Proteintech, 15910-1-AP) were stained overnight at 4°C, and the second antibody was subjected to incubation for 1 hour at ambient temperature.

Techniques:

Journal: Frontiers in Oncology

Article Title: Integrated Analysis of Transcriptome Data Revealed AURKA and KIF20A as Critical Genes in Medulloblastoma Progression

doi: 10.3389/fonc.2022.875521

Figure Lengend Snippet: KIF20A’s biological involvement in tumors. (A) KIF20A expression in different tumors. (B) KIF20A gene STRING interaction network preview of interacting proteins (showing top 20 STRING interactants). (C) Illustration of KEGG pathway analysis findings using the ClueGO plugin in Cytoscape. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: AURKA mouse monoclonal antibody (1:400, Proteintech, 66757-1-IG) and KIF20A rabbit polyclonal antibody (1:400, Proteintech, 15910-1-AP) were stained overnight at 4°C, and the second antibody was subjected to incubation for 1 hour at ambient temperature.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: Integrated Analysis of Transcriptome Data Revealed AURKA and KIF20A as Critical Genes in Medulloblastoma Progression

doi: 10.3389/fonc.2022.875521

Figure Lengend Snippet: Pathway and biological process enrichment analysis of KIF20A and top 20 interacting proteins.

Article Snippet: AURKA mouse monoclonal antibody (1:400, Proteintech, 66757-1-IG) and KIF20A rabbit polyclonal antibody (1:400, Proteintech, 15910-1-AP) were stained overnight at 4°C, and the second antibody was subjected to incubation for 1 hour at ambient temperature.

Techniques: Activity Assay

Journal: Frontiers in Oncology

Article Title: Integrated Analysis of Transcriptome Data Revealed AURKA and KIF20A as Critical Genes in Medulloblastoma Progression

doi: 10.3389/fonc.2022.875521

Figure Lengend Snippet: Immunohistochemistry analysis of AURKA and KIF20A expression in Medulloblastoma tissues from Tianjin Huanhu Hospital (normal brain, n = 4; MED, n = 10). Scale bar = 50μm. Below is a list of statistical quantitative analyses. Data are mean ± SD. ***P < 0.001, one-way ANOVA.

Article Snippet: AURKA mouse monoclonal antibody (1:400, Proteintech, 66757-1-IG) and KIF20A rabbit polyclonal antibody (1:400, Proteintech, 15910-1-AP) were stained overnight at 4°C, and the second antibody was subjected to incubation for 1 hour at ambient temperature.

Techniques: Immunohistochemistry, Expressing

Journal: Cell reports

Article Title: Genomic expansion of Aldh1a1 protects beavers against high metabolic aldehydes from lipid oxidation

doi: 10.1016/j.celrep.2021.109965

Figure Lengend Snippet: (A) The synteny of the Aldh1a1 locus in the genomes of the beaver, mouse, and human. We identified 10 copies of Aldh1a1 in the beaver genome, including 2 pseudogenes (35.15 and 266.4) and a low-quality copy (266.2). (B) Gene structure of the 7 functional copies of Aldh1a1 . (C) RNA-seq reads coverage at genomic locations of different amino acid residues among 7 Aldh1a1 gene products. Variable sites were highlighted in red, and the gray box indicates the selected sites where we checked the coverage of RNA-seq reads. RNA-seq reads from the liver tissue of 5 beaver samples (B1–B5) were used in the plot . Normalized read coverage is the read counts per 100 million mapped reads. (D) Validation of the Aldh1a1 copy number by qPCR. Two single-copy genes were used as references: Hcfc1 on an autosome and Pelo on chromosome X. Three technical replicates for each of the 3 different amounts of DNA input. Data are shown as mean ± SD. (E) Expression of different beaver Aldh1a1 copies across tissues. With an extremely high expression in liver alone, 3 copies show a liver-specific expression. (F) Aldh1a1 expression comparison between beavers and mice. The double asterisk denotes adjusted p < 0.01 after Bonferroni correction. There is no difference in the spleen tissue (nominal p = 0.53). Five biological replicates for beaver liver and skin tissues; 4 biological replicates for beaver brain, kidney, and spleen tissues. Five biological replicates for each tissue of the mouse. Data are shown as mean ± SD. (G) Western blot of Aldh1a1 protein from beaver and mouse liver extracts of 4 different samples of each species. Aldh1a1 protein quantity is statistically higher (p = 0.001 by 1-tailed Welch 2-sample t test) in the beaver liver than in the mouse liver. (H) Relative abundance of Aldh1a1 copies among detected beaver liver proteins. The Aldh1a1 copies are highly abundant. (I) Relative abundance of mouse Aldh1a1 and Aldh1a7 among all detected liver proteins. Relative protein rank is inversely correlated with abundance. The mean abundance of protein from 3 biological samples are shown in (H) and (I).

Article Snippet: Rabbit polyclonal anti-Aldh1a1 (Invitrogen cat# PA5–95937) was used as the primary to detect Aldh1a1 isoforms.

Techniques: Functional Assay, RNA Sequencing Assay, Expressing, Western Blot

Journal: Cell reports

Article Title: Genomic expansion of Aldh1a1 protects beavers against high metabolic aldehydes from lipid oxidation

doi: 10.1016/j.celrep.2021.109965

Figure Lengend Snippet: (A) Cell viability. Beaver lung fibroblasts have a lower percentage of death in the presence of high concentrations of ethanol, normalized by corresponding controls (p = 0.002 by mixed-effects model; see ). (B) Expression of Aldh1a1 in lung fibroblasts measured by qPCR with β-actin normalization. The expression of Aldh1a1 in beaver cells is higher than in mouse cells (p = 0.02, 1-tailed t test). (C) Aldehyde metabolic activity. Enhanced Aldh1a1 activity of beaver in aldehyde metabolism. Activities are normalized by corresponding controls without any addition (see ). HNE, 4-hydroxynonenal; MDA, malonaldehyde; RET, all- trans -retinal. Lung fibroblasts were used in experiments of (A) and (B). Cytosolic extracts from hepatocytes were used for the experiment of (C). Four different biological samples of each species were used in experiments of (A)–(C), with 3 technical replicates of each sample for (A) and (C), and 2 technical replicates of each sample for (B). Data are shown as mean ± SD.

Article Snippet: Rabbit polyclonal anti-Aldh1a1 (Invitrogen cat# PA5–95937) was used as the primary to detect Aldh1a1 isoforms.

Techniques: Expressing, Activity Assay

Journal: Cell reports

Article Title: Genomic expansion of Aldh1a1 protects beavers against high metabolic aldehydes from lipid oxidation

doi: 10.1016/j.celrep.2021.109965

Figure Lengend Snippet: (A) Correlation of ALDH1A1 expression with age, sex, body mass index (BMI), and post-mortem interval (PMI, minutes between death and sample collection). Tissues are ordered according to the regression coefficients on the age, as the digits show, from the highest positive coefficient to the lowest negative coefficient. The color represents p values (on a −log 10 scale), and a significant correlation (FDR < 0.01) is denoted by an asterisk. (B) Significant correlation between ALDH1A1 expression and age in 3 human tissues. The gray-shaded area around the regression line indicates 95% confidence interval.

Article Snippet: Rabbit polyclonal anti-Aldh1a1 (Invitrogen cat# PA5–95937) was used as the primary to detect Aldh1a1 isoforms.

Techniques: Expressing

Journal: Cell reports

Article Title: Genomic expansion of Aldh1a1 protects beavers against high metabolic aldehydes from lipid oxidation

doi: 10.1016/j.celrep.2021.109965

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-Aldh1a1 (Invitrogen cat# PA5–95937) was used as the primary to detect Aldh1a1 isoforms.

Techniques: Recombinant, Software

Journal: Clinical Proteomics

Article Title: Combined proteomic/transcriptomic signature of recurrence post-liver transplantation for hepatocellular carcinoma beyond Milan

doi: 10.1186/s12014-021-09333-x

Figure Lengend Snippet: Proteomics workflow and analysis of recurrent and non-recurrent hepatocellular carcinoma (HCC) tumour explant samples obtained at the time of transplantation. A Schematic diagram of the proteomics workflow including the 11 HCC samples. B Proteomics analysis workflow to identify the most significant proteins between recurrent and non-recurrent HCC. C Volcano plot illustrating proteins differentially expressed (p < 0.05) in recurrent vs non-recurrent HCC tumors. Proteins in red are significantly increased in recurrent, while those in blue are significantly decreased in the recurrent cases. The three proteins bolded and marked with stars (ALDH1A1, LGALS3 and LGALS3BP) were subsequently selected for validation. Some protein names were removed for clarity, to minimize overlap. HCC, hepatocellular carcinoma; SCX, strong cationic exchange; LC, liquid chromatography; MS/MS, tandem mass spectrometry; ALDH1A1, retinal dehydrogenase 1, LGALS3, galectin-3; LGALS3BP, galectin-3-binding protein

Article Snippet: Membranes were then blocked with 5% milk and incubated with mouse monoclonal anti-LGALS3 (1:4000; ab2785, Abcam, previously characterized [ ]) or rabbit polyclonal anti-ALDH1A1 (1:2000; ab227948, Abcam [ ]).

Techniques: Transplantation Assay, Liquid Chromatography, Tandem Mass Spectroscopy, Mass Spectrometry, Binding Assay

Journal: Clinical Proteomics

Article Title: Combined proteomic/transcriptomic signature of recurrence post-liver transplantation for hepatocellular carcinoma beyond Milan

doi: 10.1186/s12014-021-09333-x

Figure Lengend Snippet: Univariate analysis of the top proteins and genes differentially expressed in HCC explants and key clinical characteristics, as predictors of HCC recurrence post-transplant

Article Snippet: Membranes were then blocked with 5% milk and incubated with mouse monoclonal anti-LGALS3 (1:4000; ab2785, Abcam, previously characterized [ ]) or rabbit polyclonal anti-ALDH1A1 (1:2000; ab227948, Abcam [ ]).

Techniques:

Journal: Clinical Proteomics

Article Title: Combined proteomic/transcriptomic signature of recurrence post-liver transplantation for hepatocellular carcinoma beyond Milan

doi: 10.1186/s12014-021-09333-x

Figure Lengend Snippet: Kaplan–Meier and dot plots for: A ALDH1A1 gene and protein; B LGALS3 gene and protein; C LGALS3BP gene and protein in HCC patients. The levels of the gene/protein were grouped by interquartile ranges. For ALDH1A1 gene: low level ≤ 10.40; medium level [10.40–12.00]; high level ≥ 12.00. For ALDH1A1 protein: low level ≤ 28.59; medium level [28.59–30.82]; high level ≥ 30.82. For LGALS3 gene: low level ≤ 4.10; medium level [4.10–6.10]; high level ≥ 6.10. For LGALS3 protein: low level ≤ 25.88; medium level [25.88–27.80]; high level ≥ 27.80. For LGALS3BP gene: low level ≤ 9.10; medium level [9.10–10.80]; high level ≥ 10.80. For LGALS3BP protein: low level ≤ 22.69; medium level [22.69–26.09]; high level ≥ 26.09. LGALS3, galectin-3; LGALS3BP, galectin-3-binding protein

Article Snippet: Membranes were then blocked with 5% milk and incubated with mouse monoclonal anti-LGALS3 (1:4000; ab2785, Abcam, previously characterized [ ]) or rabbit polyclonal anti-ALDH1A1 (1:2000; ab227948, Abcam [ ]).

Techniques: Binding Assay

Journal: Clinical Proteomics

Article Title: Combined proteomic/transcriptomic signature of recurrence post-liver transplantation for hepatocellular carcinoma beyond Milan

doi: 10.1186/s12014-021-09333-x

Figure Lengend Snippet: Verification of ALDH1A1 and LGALS3 protein expression changes in HCC tumour explants. A Shows the immunoblot bands of ALDH1A1, LGALS3, and GAPDH in recurrent (n = 7) and non-recurrent (n = 4) HCC patients. Cropped images of the blots were used in order to improve the clarity and conciseness of the presentation. The protein expression of ALDH1A1 ( B ) and LGALS3 ( C ) were measured by densitometry and normalized to GAPDH. The Pearson correlations between Western Blot and mass spectrometry log2-transformed LFQ protein intensity values of ALDH1A1 and LGALS3 were evaluated ( D ). The Western blot LGALS3/ALDH1A1 ratio was also calculated ( E ). *P < 0.05. Data are reported as mean ± standard error. LFQ, label-free quantification; LGALS3, galectin-3; ALDH1A1, retinal dehydrogenase 1. LGALS3, galectin-3; LGALS3BP, galectin-3-binding protein; ALDH1A1, retinal dehydrogenase 1

Article Snippet: Membranes were then blocked with 5% milk and incubated with mouse monoclonal anti-LGALS3 (1:4000; ab2785, Abcam, previously characterized [ ]) or rabbit polyclonal anti-ALDH1A1 (1:2000; ab227948, Abcam [ ]).

Techniques: Expressing, Western Blot, Mass Spectrometry, Transformation Assay, Binding Assay