rabbit anti active caspase 3 igg  (R&D Systems)

Journal: Antibiotics

Article Title: Silver Nanoparticles Phytofabricated through Azadirachta indica : Anticancer, Apoptotic, and Wound-Healing Properties

doi: 10.3390/antibiotics12010121

Figure Lengend Snippet: Caspase-3 activities induced by biosynthesized silver nanoparticles in HCT-116 cells, quantified through FITC rabbit anti-active caspase-3 IgG antibody (BD Biosciences). Representative FS/SS dot plots ( a ) of uniform population, percentage of cells with high caspase-3 activity assessed by dUTP-FITC ( b ) for control (i), standard EGF (ii), and biosynthesized silver nanoparticles (AgNPs) (iii). Flow cytometry histogram overlays ( c ) show the intensity of dUTP-FITC in the HCT-116 cells that are untreated (control), those treated with cisplatin (standard), and those treated with biosynthesized silver nanoparticles (AgNPs).

Article Snippet: The APO-DIRECT™ kit and FITC rabbit anti-active caspase-3 IgG antibody were purchased from Pharmingen (BD Biosciences).

Techniques: Activity Assay, Flow Cytometry

Journal: Antibiotics

Article Title: Silver Nanoparticles Phytofabricated through Azadirachta indica : Anticancer, Apoptotic, and Wound-Healing Properties

doi: 10.3390/antibiotics12010121

Figure Lengend Snippet: Caspase-3 pathway of cellular apoptosis.

Article Snippet: The APO-DIRECT™ kit and FITC rabbit anti-active caspase-3 IgG antibody were purchased from Pharmingen (BD Biosciences).

Techniques:

Journal: PLoS ONE

Article Title: In Vitro Phenotypic, Genomic and Proteomic Characterization of a Cytokine-Resistant Murine β-TC3 Cell Line

doi: 10.1371/journal.pone.0032109

Figure Lengend Snippet: ( A ) Flow cytometry analysis of cell-cycle in β-TC3 and β-TC3R cells. Green line indicates cycle profile in untreated cells, while red line indicates cell cycle after treatment with cytokines. ( B ) Effects of cytokines on DNA fragmentation in β-TC3 and β-TC3R cells. M = DNA laddering Marker (100 bp). ( C ) Flow cytometry analysis of Caspase 3 in β-TC3 and β-TC3R cells after treatment with cytokines. No significant change was observed in β-TC3R cells. Cytokine treatment is 100 IU/ml of IL-1β + IFN-γ for 72 hours. U = untreated.

Article Snippet: For Caspase 3 assay, cells were first fixed and permeabilized with Cytofix-Cytoperm kit (BD Pharmingen), incubated with monoclonal IgG rabbit anti-Active Caspase 3 (BD Pharmingen) and fluorescein isothiocyanate (FITC)-conjugated polyclonal goat anti-rabbit IgG (Santa Cruz Biotechnology), according to the manufacturer's instructions.

Techniques: Flow Cytometry, DNA Laddering, Marker