rabbit anti acyl coa synthetase longchain family member 4  (Boster Bio)

Journal: Current Issues in Molecular Biology

Article Title: Protective Effect of Hepcidin on Sepsis-Associated Acute Kidney Injury via Activating the Nrf2/GPX4 Signaling Pathway

doi: 10.3390/cimb47090772

Figure Lengend Snippet: Hepcidin inhibits ferroptosis in SAKI mice. ( A ) The content of ferrous iron in renal tissues ( n = 5 mice per group); ( B ) Renal tissue MDA levels ( n = 5 mice per group); ( C ) GSH content in renal tissues ( n = 5 mice per group); ( D ) GSH/GSSG ratio; ( E ) GSH-Px activity ( n = 5 mice per group); ( F ) Ferroptosis-dependent protein expression changes in mice; Relative expression levels of ACSL4 ( G ) and GPX4 ( H ) in renal tissue ( n = 3 mice per group); Compared with the sham group, ns, no significant difference; **** p < 0.0001; Compared with the CLP group, # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001. Hamp, hepcidin; Fer-1, Ferrostatin-1.

Article Snippet: After incubation in blocking buffer for 30 min, the PVDF membranes were incubated overnight at 4 °C with a mouse antibody against GPX4 (1:2000, 67763-1-Ig, Proteintech, Wuhan, China), a rabbit antibody against ACSL4 (1:10,000, 22401-1-AP, Proteintech, Wuhan, China), a rabbit antibody against Nrf2 (1:1000, A0674, ABclonal, Wuhan, China), a rabbit antibody against Lamin B1 (1:10,000, 12987-1-AP, Proteintech, Wuhan, China), and a mouse antibody against GAPDH (1:50,000, 60004-1-Ig, Proteintech, Wuhan, China).

Techniques: Activity Assay, Expressing