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LJP attenuates OGD/R-induced inflammation in BV2 microglia via Csf3. (A) CCK-8 assay of cell viability. (B, C) . Intracellular ROS levels were measured by DCFH-DA fluorescence. (D) qPCR shows the expression of Csf3, IL-1b, Tnfα and IL-6 in OGD/R-stimulated BV2 cells. (E) Representative immunofluorescence images of Csf3 (red) and DAPI (blue) in BV2 cells. (F) ELISA present the levels of IL-1b, Tnfα and IL-6 were detected in the supernatant of OGD/R-stimulated BV2 cells (n = 4).

Journal: Frontiers in Immunology

Article Title: Laminaria japonica polysaccharide mitigates acute neuroinflammation in cerebral ischemia-reperfusion injury through Csf3-modulated pathways

doi: 10.3389/fimmu.2026.1801746

Figure Lengend Snippet: LJP attenuates OGD/R-induced inflammation in BV2 microglia via Csf3. (A) CCK-8 assay of cell viability. (B, C) . Intracellular ROS levels were measured by DCFH-DA fluorescence. (D) qPCR shows the expression of Csf3, IL-1b, Tnfα and IL-6 in OGD/R-stimulated BV2 cells. (E) Representative immunofluorescence images of Csf3 (red) and DAPI (blue) in BV2 cells. (F) ELISA present the levels of IL-1b, Tnfα and IL-6 were detected in the supernatant of OGD/R-stimulated BV2 cells (n = 4).

Article Snippet: Cell Culture and OGD/R Model: BV2 microglia (Procell Life Science) were cultured in high-glucose DMEM supplemented with 10% FBS and 1% penicillin/streptomycin under standard conditions (37 °C, 5% CO2).

Techniques: CCK-8 Assay, Fluorescence, Expressing, Immunofluorescence, Enzyme-linked Immunosorbent Assay