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Stratagene quikchange pcr strategy
Quikchange Pcr Strategy, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/quikchange pcr strategy/product/Stratagene
Average 88 stars, based on 4 article reviews
Price from $9.99 to $1999.99
quikchange pcr strategy - by Bioz Stars, 2020-07
88/100 stars

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Positron Emission Tomography:

Article Title: Structure and Catalysis in the Escherichiacoli Hotdog-fold Thioesterase Paralogs YdiI and YbdB
Article Snippet: .. Preparation of YbdB and YdiI Site-Directed Mutants Site directed mutagenesis was carried out using the QuikChange PCR strategy (Stratagene) with the ydiI-His 6 /pET-23a or ybdB-His 6 /pET-23a plasmid as template and commercial primers and Pfu Turbo as the polymerase. ..

Polymerase Chain Reaction:

Article Title: SV2 regulates neurotransmitter release via multiple mechanisms
Article Snippet: .. SV2A mutations were made in pIRES2-EGFP (internal ribosomal entry sequence 2-enhanced green fluorescent protein), using a QuikChange PCR strategy (Stratagene). .. The IRES sequence was removed to produce SV2A fused to EGFP, which would later act as a real-time visual reporter of infection.

Article Title: Structure and Catalysis in the Escherichiacoli Hotdog-fold Thioesterase Paralogs YdiI and YbdB
Article Snippet: .. Preparation of YbdB and YdiI Site-Directed Mutants Site directed mutagenesis was carried out using the QuikChange PCR strategy (Stratagene) with the ydiI-His 6 /pET-23a or ybdB-His 6 /pET-23a plasmid as template and commercial primers and Pfu Turbo as the polymerase. ..

Article Title: A Novel Role for γ-Secretase: Selective Regulation of Spontaneous Neurotransmitter Release from Hippocampal Neurons
Article Snippet: .. Point mutations in PS1 were introduced using the QuikChange PCR strategy (Stratagene) and confirmed by sequencing. .. Lenti virions were generated according to the Invitrogen ViraPower Lentiviral Expression System manual using the lentivirus plasmid pRRL-cPPT-CMV-X-PRE-SIN ( ).

Article Title: Regulation of CB1 cannabinoid receptor internalization by a promiscuous phosphorylation-dependent mechanism
Article Snippet: .. CB1 receptor mutants T461A/S463A, S465A/T466A, T468A/S469A, T461A–T466A and T461A–S469A were made using a modified QuikChange PCR strategy (Stratagene, La Jolla, CA). .. Full-length rat CB1 , with an amino-terminal pplssHA (prolactin signal sequence and hemagglutinin epitope) tag ( ) in pcDNA3.0 (Invitrogen, Carlsbad, CA), was used as a template to generate the T461A/S463A, S465A/T466A and T468A/S469A mutant constructs.

Article Title: Rapid CB1 cannabinoid receptor desensitization defines the time course of ERK1/2 MAP kinase signaling
Article Snippet: .. The CB1 receptor mutant, S426A/S430A, was made using a modified QuikChange PCR strategy (Stratagene, La Jolla, CA) to introduce serine to alanine point mutations. .. Full-length rat CB1 , with an amino-terminal pplssHA (prolactin signal sequence and hemagglutinin epitope) tag ( ) in pcDNA3.0 (Invitrogen, Carlsbad, CA), was used as a template with the following primers: forward 5′ccgcacagcctcttgacaacgccatgggggacgcagactgcctgc-3′ and reverse 5′-gcaggcagtctgcgtcccccatggcgttgtcaagaggctgtgcgg-3′.

Mutagenesis:

Article Title: Structure and Catalysis in the Escherichiacoli Hotdog-fold Thioesterase Paralogs YdiI and YbdB
Article Snippet: .. Preparation of YbdB and YdiI Site-Directed Mutants Site directed mutagenesis was carried out using the QuikChange PCR strategy (Stratagene) with the ydiI-His 6 /pET-23a or ybdB-His 6 /pET-23a plasmid as template and commercial primers and Pfu Turbo as the polymerase. ..

Article Title: Rapid CB1 cannabinoid receptor desensitization defines the time course of ERK1/2 MAP kinase signaling
Article Snippet: .. The CB1 receptor mutant, S426A/S430A, was made using a modified QuikChange PCR strategy (Stratagene, La Jolla, CA) to introduce serine to alanine point mutations. .. Full-length rat CB1 , with an amino-terminal pplssHA (prolactin signal sequence and hemagglutinin epitope) tag ( ) in pcDNA3.0 (Invitrogen, Carlsbad, CA), was used as a template with the following primers: forward 5′ccgcacagcctcttgacaacgccatgggggacgcagactgcctgc-3′ and reverse 5′-gcaggcagtctgcgtcccccatggcgttgtcaagaggctgtgcgg-3′.

Introduce:

Article Title: Rapid CB1 cannabinoid receptor desensitization defines the time course of ERK1/2 MAP kinase signaling
Article Snippet: .. The CB1 receptor mutant, S426A/S430A, was made using a modified QuikChange PCR strategy (Stratagene, La Jolla, CA) to introduce serine to alanine point mutations. .. Full-length rat CB1 , with an amino-terminal pplssHA (prolactin signal sequence and hemagglutinin epitope) tag ( ) in pcDNA3.0 (Invitrogen, Carlsbad, CA), was used as a template with the following primers: forward 5′ccgcacagcctcttgacaacgccatgggggacgcagactgcctgc-3′ and reverse 5′-gcaggcagtctgcgtcccccatggcgttgtcaagaggctgtgcgg-3′.

Sequencing:

Article Title: SV2 regulates neurotransmitter release via multiple mechanisms
Article Snippet: .. SV2A mutations were made in pIRES2-EGFP (internal ribosomal entry sequence 2-enhanced green fluorescent protein), using a QuikChange PCR strategy (Stratagene). .. The IRES sequence was removed to produce SV2A fused to EGFP, which would later act as a real-time visual reporter of infection.

Article Title: A Novel Role for γ-Secretase: Selective Regulation of Spontaneous Neurotransmitter Release from Hippocampal Neurons
Article Snippet: .. Point mutations in PS1 were introduced using the QuikChange PCR strategy (Stratagene) and confirmed by sequencing. .. Lenti virions were generated according to the Invitrogen ViraPower Lentiviral Expression System manual using the lentivirus plasmid pRRL-cPPT-CMV-X-PRE-SIN ( ).

Modification:

Article Title: Regulation of CB1 cannabinoid receptor internalization by a promiscuous phosphorylation-dependent mechanism
Article Snippet: .. CB1 receptor mutants T461A/S463A, S465A/T466A, T468A/S469A, T461A–T466A and T461A–S469A were made using a modified QuikChange PCR strategy (Stratagene, La Jolla, CA). .. Full-length rat CB1 , with an amino-terminal pplssHA (prolactin signal sequence and hemagglutinin epitope) tag ( ) in pcDNA3.0 (Invitrogen, Carlsbad, CA), was used as a template to generate the T461A/S463A, S465A/T466A and T468A/S469A mutant constructs.

Article Title: Rapid CB1 cannabinoid receptor desensitization defines the time course of ERK1/2 MAP kinase signaling
Article Snippet: .. The CB1 receptor mutant, S426A/S430A, was made using a modified QuikChange PCR strategy (Stratagene, La Jolla, CA) to introduce serine to alanine point mutations. .. Full-length rat CB1 , with an amino-terminal pplssHA (prolactin signal sequence and hemagglutinin epitope) tag ( ) in pcDNA3.0 (Invitrogen, Carlsbad, CA), was used as a template with the following primers: forward 5′ccgcacagcctcttgacaacgccatgggggacgcagactgcctgc-3′ and reverse 5′-gcaggcagtctgcgtcccccatggcgttgtcaagaggctgtgcgg-3′.

Plasmid Preparation:

Article Title: Structure and Catalysis in the Escherichiacoli Hotdog-fold Thioesterase Paralogs YdiI and YbdB
Article Snippet: .. Preparation of YbdB and YdiI Site-Directed Mutants Site directed mutagenesis was carried out using the QuikChange PCR strategy (Stratagene) with the ydiI-His 6 /pET-23a or ybdB-His 6 /pET-23a plasmid as template and commercial primers and Pfu Turbo as the polymerase. ..

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  • 95
    Stratagene quikchange site directed mutagenesis kit
    Quikchange Site Directed Mutagenesis Kit, supplied by Stratagene, used in various techniques. Bioz Stars score: 95/100, based on 3351 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quikchange site directed mutagenesis kit/product/Stratagene
    Average 95 stars, based on 3351 article reviews
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    quikchange site directed mutagenesis kit - by Bioz Stars, 2020-07
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    88
    Stratagene quikchange pcr strategy
    Quikchange Pcr Strategy, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quikchange pcr strategy/product/Stratagene
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    quikchange pcr strategy - by Bioz Stars, 2020-07
    88/100 stars
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    86
    Stratagene pcr based quikchangetm strategy
    Pcr Based Quikchangetm Strategy, supplied by Stratagene, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    pcr based quikchangetm strategy - by Bioz Stars, 2020-07
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