quikchange ii xl site directed mutagenesis kit  (Stratagene)

 
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    Structured Review

    Stratagene quikchange ii xl site directed mutagenesis kit
    Quikchange Ii Xl Site Directed Mutagenesis Kit, supplied by Stratagene, used in various techniques. Bioz Stars score: 94/100, based on 1290 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quikchange ii xl site directed mutagenesis kit/product/Stratagene
    Average 94 stars, based on 1290 article reviews
    Price from $9.99 to $1999.99
    quikchange ii xl site directed mutagenesis kit - by Bioz Stars, 2020-09
    94/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: The Weak Complex between RhoGAP Protein ARHGAP22 and Signal Regulatory Protein 14-3-3 Has 1:2 Stoichiometry and a Single Peptide Binding Mode
    Article Snippet: .. The double mutant S16D/S411D of AG22 (1–422) was generated using the Quikchange II XL site-directed mutagenesis kit following manufacturer's instructions (Stratagene, USA). .. The codon-optimized synthetic gene of human 14-3-3 β isoform (GeneArt) was subcloned into pET20b encoding a C-terminal hexa-histidine tag.

    Article Title: USP29 is a novel non-canonical Hypoxia Inducible Factor-α activator
    Article Snippet: .. HIF-α truncations (HIF-1α630-713 and HIF-1α630-750 ) as well as HIF-1α DMK752/755/758R , HIF-2α DM(P405/531A) , HA-USP29 siRNA-resistant and the catalytically inactive USP29C294S were generated using the QuikChange® II XL Site-Directed Mutagenesis Kit (Stratagene) and the oligos reported in . ..

    Article Title: Repertoire of antibodies against type 1 poliovirus in human sera.
    Article Snippet: .. A blocking-ELISA procedure was used to quantify antibodies in sera of humans immunized with poliovirus vaccines. .. A blocking-ELISA procedure was used to quantify antibodies in sera of humans immunized with poliovirus vaccines.

    Article Title: Ligand and antagonist driven regulation of the Vibrio cholerae quorum-sensing receptor CqsS
    Article Snippet: .. Plasmids encoding CqsS H194Q (CqsS His- ), CqsS D618N (CqsS Asp- ), CqsS G379A/G381A (CqsS Cat- ), CqsSC170Y and CqsSF162A were constructed from the plasmid containing wild-type CqsS using the Quikchange II XL Site-Directed Mutagenesis Kit (Stratagene). .. The genes encoding LuxU and LuxO were PCR amplified from V. cholerae genomic DNA and cloned into the pET28b vector.

    Article Title: The chimeric gene CHRFAM7A, a partial duplication of the CHRNA7 gene, is a dominant negative regulator of α7*nAChR function
    Article Snippet: .. The 2bp deletion in exon 6 of CHRFAM7A was introduced into pcDNA3.1 CHRFAM7A by PCR with the QuikChange II XL Site-Directed Mutagenesis Kit, according to the manufacturer's protocols (Stratagene, La Jolly, CA). .. All clones were sequenced bidirectionally using internal primers and dRhodamine dye terminators on an ABI 3100 Avant DNA sequencer (Applied Biosytems, Inc., Foster City, CA) for confirmation of correct sequence.

    Article Title: Drosophila canopy b is a cochaperone of glycoprotein 93
    Article Snippet: .. A Stratagene QuikChange II XL site-directed mutagenesis kit was used to create all CNPYb mutants, following the manufacturer's protocol. .. Primers were designed with the Stratagene online primer design program, and mutants were PCR-amplified from wild-type CNPYb myc-MigR1.

    Article Title: A PP4 phosphatase complex dephosphorylates RPA2 to facilitate DNA repair via homologous recombination
    Article Snippet: .. We constructed phosphomimetic mutants (RPA2 S33D, RPA2 S8D, RPA2 S33D S8D), PP4C mutant (PP4C D82A) and PP4R2 mutants (PP4R2 F99A, PP4R2 R103A, PP4R2 E106A) by QuikChange II XL site-directed mutagenesis kit (Stratagene) according to the manufacturer’s instructions. ..

    Article Title: Selective determinants of inositol 1,4,5-trisphosphate and adenophostin A interactions with type 1 inositol 1,4,5-trisphosphate receptors
    Article Snippet: .. The QuikChange II XL site-directed mutagenesis kit was used to introduce point mutations into the IBC and NT constructs in pTrcHisA vectors using the primers listed in the preceding section. .. Expression of fragments of IP3 R1 His6 -tagged IBC and NT fragments were expressed as described previously ( ).

    Construct:

    Article Title: Ligand and antagonist driven regulation of the Vibrio cholerae quorum-sensing receptor CqsS
    Article Snippet: .. Plasmids encoding CqsS H194Q (CqsS His- ), CqsS D618N (CqsS Asp- ), CqsS G379A/G381A (CqsS Cat- ), CqsSC170Y and CqsSF162A were constructed from the plasmid containing wild-type CqsS using the Quikchange II XL Site-Directed Mutagenesis Kit (Stratagene). .. The genes encoding LuxU and LuxO were PCR amplified from V. cholerae genomic DNA and cloned into the pET28b vector.

    Article Title: A PP4 phosphatase complex dephosphorylates RPA2 to facilitate DNA repair via homologous recombination
    Article Snippet: .. We constructed phosphomimetic mutants (RPA2 S33D, RPA2 S8D, RPA2 S33D S8D), PP4C mutant (PP4C D82A) and PP4R2 mutants (PP4R2 F99A, PP4R2 R103A, PP4R2 E106A) by QuikChange II XL site-directed mutagenesis kit (Stratagene) according to the manufacturer’s instructions. ..

    Article Title: Selective determinants of inositol 1,4,5-trisphosphate and adenophostin A interactions with type 1 inositol 1,4,5-trisphosphate receptors
    Article Snippet: .. The QuikChange II XL site-directed mutagenesis kit was used to introduce point mutations into the IBC and NT constructs in pTrcHisA vectors using the primers listed in the preceding section. .. Expression of fragments of IP3 R1 His6 -tagged IBC and NT fragments were expressed as described previously ( ).

    Generated:

    Article Title: The Weak Complex between RhoGAP Protein ARHGAP22 and Signal Regulatory Protein 14-3-3 Has 1:2 Stoichiometry and a Single Peptide Binding Mode
    Article Snippet: .. The double mutant S16D/S411D of AG22 (1–422) was generated using the Quikchange II XL site-directed mutagenesis kit following manufacturer's instructions (Stratagene, USA). .. The codon-optimized synthetic gene of human 14-3-3 β isoform (GeneArt) was subcloned into pET20b encoding a C-terminal hexa-histidine tag.

    Article Title: USP29 is a novel non-canonical Hypoxia Inducible Factor-α activator
    Article Snippet: .. HIF-α truncations (HIF-1α630-713 and HIF-1α630-750 ) as well as HIF-1α DMK752/755/758R , HIF-2α DM(P405/531A) , HA-USP29 siRNA-resistant and the catalytically inactive USP29C294S were generated using the QuikChange® II XL Site-Directed Mutagenesis Kit (Stratagene) and the oligos reported in . ..

    Introduce:

    Article Title: Selective determinants of inositol 1,4,5-trisphosphate and adenophostin A interactions with type 1 inositol 1,4,5-trisphosphate receptors
    Article Snippet: .. The QuikChange II XL site-directed mutagenesis kit was used to introduce point mutations into the IBC and NT constructs in pTrcHisA vectors using the primers listed in the preceding section. .. Expression of fragments of IP3 R1 His6 -tagged IBC and NT fragments were expressed as described previously ( ).

    Polymerase Chain Reaction:

    Article Title: The chimeric gene CHRFAM7A, a partial duplication of the CHRNA7 gene, is a dominant negative regulator of α7*nAChR function
    Article Snippet: .. The 2bp deletion in exon 6 of CHRFAM7A was introduced into pcDNA3.1 CHRFAM7A by PCR with the QuikChange II XL Site-Directed Mutagenesis Kit, according to the manufacturer's protocols (Stratagene, La Jolly, CA). .. All clones were sequenced bidirectionally using internal primers and dRhodamine dye terminators on an ABI 3100 Avant DNA sequencer (Applied Biosytems, Inc., Foster City, CA) for confirmation of correct sequence.

    Plasmid Preparation:

    Article Title: Ligand and antagonist driven regulation of the Vibrio cholerae quorum-sensing receptor CqsS
    Article Snippet: .. Plasmids encoding CqsS H194Q (CqsS His- ), CqsS D618N (CqsS Asp- ), CqsS G379A/G381A (CqsS Cat- ), CqsSC170Y and CqsSF162A were constructed from the plasmid containing wild-type CqsS using the Quikchange II XL Site-Directed Mutagenesis Kit (Stratagene). .. The genes encoding LuxU and LuxO were PCR amplified from V. cholerae genomic DNA and cloned into the pET28b vector.

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  • 94
    Stratagene quikchange ii xl site directed mutagenesis kit
    Quikchange Ii Xl Site Directed Mutagenesis Kit, supplied by Stratagene, used in various techniques. Bioz Stars score: 94/100, based on 1290 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quikchange ii xl site directed mutagenesis kit/product/Stratagene
    Average 94 stars, based on 1290 article reviews
    Price from $9.99 to $1999.99
    quikchange ii xl site directed mutagenesis kit - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

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