px330 vector (New England Biolabs)
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New England Biolabs
px330 vector
Px330 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 515 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/px330 vector/product/New England Biolabs
Average 91 stars, based on 515 article reviews
Price from $9.99 to $1999.99
Px330 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 515 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/px330 vector/product/New England Biolabs
Average 91 stars, based on 515 article reviews
Price from $9.99 to $1999.99
px330 vector - by Bioz Stars,
2020-05
91/100 stars
Related Products / Commonly Used Together
Images
1) Product Images from "Generation of WNK1 knockout cell lines by CRISPR/Cas-mediated genome editing"
Article Title: Generation of WNK1 knockout cell lines by CRISPR/Cas-mediated genome editing
Journal: American Journal of Physiology - Renal Physiology
doi: 10.1152/ajprenal.00612.2014
Figure Legend Snippet: Generation and validation of WNK1 knockout (KO) cell lines. A : mismatch-specific endonuclease assay. Genomic PCR (gPCR) products spanning exon 1 of WNK1 were amplified from the template of a heterogeneous population of HEK293T cells transfected with px330-WNK1
Techniques Used: Knock-Out, Polymerase Chain Reaction, Amplification, Transfection
Related Articles
In Vitro:Article Title: A Simple and Cost-Effective Approach for In Vitro Production of Sliced siRNAs as Potent Triggers for RNAi Article Snippet: .. The protocol was modified as follows when CIP or T4 PNK treatment is necessary: (1) for CIP treatment, in 20 μL of products from one in vitro transcription reaction before DNase treatment, we added 1 μL of DNase (supplied with T7 Transcription Kit), 1 μL of CIP, 4 μL of 10× CutSmart buffer (NEB), and water to total volume of 40 μL, and incubated at 37°C for 15 min; and (2) for T4 PNK treatment, in 20 μL of products from one in vitro transcription reaction before DNase treatment, we added 1 μL of DNase (supplied with T7 Transcription Kit), 1 μL of T4 PNK, 4 μL of 10× Isolation:Article Title: Deep small RNA sequencing from the nematode Ascaris reveals conservation, functional diversification, and novel developmental profiles Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen), and small RNA samples were 5′ labeled by first treating with calf alkaline phosphatase (Roche) followed by phosphorylation with Labeling:Article Title: Deep small RNA sequencing from the nematode Ascaris reveals conservation, functional diversification, and novel developmental profiles Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen), and small RNA samples were 5′ labeled by first treating with calf alkaline phosphatase (Roche) followed by phosphorylation with Article Title: Kaposi's Sarcoma-Associated Herpesvirus Rta Tetramers Make High-Affinity Interactions with Repetitive DNA Elements in the Mta Promoter To Stimulate DNA Binding of RBP-Jk/CSL ▿Kaposi's Sarcoma-Associated Herpesvirus Rta Tetramers Make High-Affinity Interactions with Repetitive DNA Elements in the Mta Promoter To Stimulate DNA Binding of RBP-Jk/CSL ▿ † Article Snippet: .. Five hundred nanograms of probe was end labeled using 10 units Purification:Article Title: A Simple and Cost-Effective Approach for In Vitro Production of Sliced siRNAs as Potent Triggers for RNAi Article Snippet: .. The protocol was modified as follows when CIP or T4 PNK treatment is necessary: (1) for CIP treatment, in 20 μL of products from one in vitro transcription reaction before DNase treatment, we added 1 μL of DNase (supplied with T7 Transcription Kit), 1 μL of CIP, 4 μL of 10× CutSmart buffer (NEB), and water to total volume of 40 μL, and incubated at 37°C for 15 min; and (2) for T4 PNK treatment, in 20 μL of products from one in vitro transcription reaction before DNase treatment, we added 1 μL of DNase (supplied with T7 Transcription Kit), 1 μL of T4 PNK, 4 μL of 10× Produced:Article Title: PARP3 is a sensor of nicked nucleosomes and monoribosylates histone H2BGlu2 Article Snippet: .. DNA from the MNase concentration that produced the greatest SSB/DSB ratio (0.015 U) was mock-treated or treated with Concentration Assay:Article Title: PARP3 is a sensor of nicked nucleosomes and monoribosylates histone H2BGlu2 Article Snippet: .. DNA from the MNase concentration that produced the greatest SSB/DSB ratio (0.015 U) was mock-treated or treated with Incubation:Article Title: A Simple and Cost-Effective Approach for In Vitro Production of Sliced siRNAs as Potent Triggers for RNAi Article Snippet: .. The protocol was modified as follows when CIP or T4 PNK treatment is necessary: (1) for CIP treatment, in 20 μL of products from one in vitro transcription reaction before DNase treatment, we added 1 μL of DNase (supplied with T7 Transcription Kit), 1 μL of CIP, 4 μL of 10× CutSmart buffer (NEB), and water to total volume of 40 μL, and incubated at 37°C for 15 min; and (2) for T4 PNK treatment, in 20 μL of products from one in vitro transcription reaction before DNase treatment, we added 1 μL of DNase (supplied with T7 Transcription Kit), 1 μL of T4 PNK, 4 μL of 10× Article Title: Crosslinking of Ribosomal Proteins to RNA in Maize Ribosomes by UV-B and Its Effects on Translation 1Crosslinking of Ribosomal Proteins to RNA in Maize Ribosomes by UV-B and Its Effects on Translation 1 [w] Article Snippet: .. The pellet was resuspended in the polynucleotide kinase buffer (New England Biolabs, Beverly, MA), and 5 μ g of protein was incubated with 50 μ Ci of [ γ 32 P] dATP in the presence of 10 units of Article Title: Usb1 controls U6 snRNP assembly through evolutionarily divergent cyclic phosphodiesterase activities Article Snippet: .. Samples were treated with CIP or other:Article Title: Distinct and overlapping roles for two Dicer-like proteins in the RNA interference pathways of the ancient eukaryote Trypanosoma brucei Article Snippet: Small RNAs were size-selected ( ) and treated with a variety of enzymes under manufacturer-recommended conditions: Calf intestinal alkaline phosphatase (CIP; Amersham) and Modification:Article Title: A Simple and Cost-Effective Approach for In Vitro Production of Sliced siRNAs as Potent Triggers for RNAi Article Snippet: .. The protocol was modified as follows when CIP or T4 PNK treatment is necessary: (1) for CIP treatment, in 20 μL of products from one in vitro transcription reaction before DNase treatment, we added 1 μL of DNase (supplied with T7 Transcription Kit), 1 μL of CIP, 4 μL of 10× CutSmart buffer (NEB), and water to total volume of 40 μL, and incubated at 37°C for 15 min; and (2) for T4 PNK treatment, in 20 μL of products from one in vitro transcription reaction before DNase treatment, we added 1 μL of DNase (supplied with T7 Transcription Kit), 1 μL of T4 PNK, 4 μL of 10× |