quick load purple low molecular weight dna ladder  (New England Biolabs)


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    Name:
    Quick Load Purple Low Molecular Weight DNA Ladder
    Description:
    Quick Load Purple Low Molecular Weight DNA Ladder 125 gel lanes
    Catalog Number:
    n0557s
    Price:
    88
    Size:
    125 gel lanes
    Category:
    DNA Ladders
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    Structured Review

    New England Biolabs quick load purple low molecular weight dna ladder
    Quick Load Purple Low Molecular Weight DNA Ladder
    Quick Load Purple Low Molecular Weight DNA Ladder 125 gel lanes
    https://www.bioz.com/result/quick load purple low molecular weight dna ladder/product/New England Biolabs
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    quick load purple low molecular weight dna ladder - by Bioz Stars, 2021-01
    94/100 stars

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    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: Systematic evaluation and optimization of the experimental steps in RNA G-quadruplex structure sequencing
    Article Snippet: .. PCR samples were added with 6X orange loading dye (NEB, B7022S) and then resolved on a 1.8% TAE agarose gel at 120 V, 55 min with 4 μl Quick-load Purple LMW marker (NEB, N0557S) included. .. The 150–400 nt region of each PCR product was cut and purified by using the Zymo DNA agarose gel extraction kit (Zymo, D4008) following manufacturer’s protocol.

    Marker:

    Article Title: Validation of use of the miniPCR thermocycler for Ebola and Zika virus detection
    Article Snippet: .. We used the Quick-Load Purple 2-Log DNA Ladder (NEB, MA, USA) as molecular a molecular weight marker. .. We analyzed the gels by UV transillumination using a Bio-Rad ChemiDoc XRS imaging system.

    Article Title: Portable and accurate diagnostics for COVID-19: Combined use of the miniPCR thermocycler and a well-plate reader for SARS-CoV-2 virus detection
    Article Snippet: .. Gels were dyed with GelGreen (Biotium, CA, USA) using a 1:10,000 dilution and a current of 110 V supplied by a Bio-Rad PowerPac HC power supply (Bio-Rad, CA, USA) for 40 min. We used the Quick-Load Purple 2-Log DNA ladder (NEB, MA, USA) as a molecular weight marker. .. We analyzed the gels by UV transillumination using a Bio-Rad ChemiDoc XRS imaging system.

    Article Title: Scaling diagnostics in times of COVID-19: Colorimetric Loop-mediated Isothermal Amplification (LAMP) assisted by a 3D-printed incubator for cost-effective and scalable detection of SARS-CoV-2
    Article Snippet: .. We used the Quick-Load Purple 2-Log DNA Ladder 543 (NEB, MA, USA) as a molecular weight marker. .. Gels were dyed with Gel-Green from 544 Biotium (CA, USA) using a 1:10,000 dilution, and a current of 48 V was supplied by the 545 blueGel built-in power supply (AC 100–240V, 50–60Hz).

    Article Title: Systematic evaluation and optimization of the experimental steps in RNA G-quadruplex structure sequencing
    Article Snippet: .. PCR samples were added with 6X orange loading dye (NEB, B7022S) and then resolved on a 1.8% TAE agarose gel at 120 V, 55 min with 4 μl Quick-load Purple LMW marker (NEB, N0557S) included. .. The 150–400 nt region of each PCR product was cut and purified by using the Zymo DNA agarose gel extraction kit (Zymo, D4008) following manufacturer’s protocol.

    Molecular Weight:

    Article Title: Validation of use of the miniPCR thermocycler for Ebola and Zika virus detection
    Article Snippet: .. We used the Quick-Load Purple 2-Log DNA Ladder (NEB, MA, USA) as molecular a molecular weight marker. .. We analyzed the gels by UV transillumination using a Bio-Rad ChemiDoc XRS imaging system.

    Article Title: Immuno-SABER enables highly multiplexed and amplified protein imaging in tissues
    Article Snippet: .. 160 ng Quick-Load Purple Low Molecular Weight DNA Ladder (NEB #N0557S) was loaded as size reference. ..

    Article Title: Portable and accurate diagnostics for COVID-19: Combined use of the miniPCR thermocycler and a well-plate reader for SARS-CoV-2 virus detection
    Article Snippet: .. Gels were dyed with GelGreen (Biotium, CA, USA) using a 1:10,000 dilution and a current of 110 V supplied by a Bio-Rad PowerPac HC power supply (Bio-Rad, CA, USA) for 40 min. We used the Quick-Load Purple 2-Log DNA ladder (NEB, MA, USA) as a molecular weight marker. .. We analyzed the gels by UV transillumination using a Bio-Rad ChemiDoc XRS imaging system.

    Article Title: Scaling diagnostics in times of COVID-19: Colorimetric Loop-mediated Isothermal Amplification (LAMP) assisted by a 3D-printed incubator for cost-effective and scalable detection of SARS-CoV-2
    Article Snippet: .. We used the Quick-Load Purple 2-Log DNA Ladder 543 (NEB, MA, USA) as a molecular weight marker. .. Gels were dyed with Gel-Green from 544 Biotium (CA, USA) using a 1:10,000 dilution, and a current of 48 V was supplied by the 545 blueGel built-in power supply (AC 100–240V, 50–60Hz).

    Article Title: Using CAPTURE to detect spacer acquisition in native CRISPR arrays
    Article Snippet: .. OneTaq® Quick-Load® 2X Master Mix with Standard Buffer (Bioké, cat. no. M0486S) Agarose (Promega, cat no. V3125) 40X TAE buffer stock (Promega, cat no. V4281) SYBR Safe (Thermo Fisher Scientific, cat no. ) SYBR Gold (Thermo Fisher Scientific, cat no. S11494) SmartLadder SF (Eurogentec, cat no. MW-1800-04) Quick-Load® Purple Low Molecular Weight DNA Ladder (NEB, cat no. N0557S) Primer sets ( , , and .) (Integrated DNA Technologies) Gibco Distilled Water (Thermo Fisher Scientific, cat. No. 10977035) Absolute Ethanol (Sigma Aldrich, cat. no. 46139) CAUTION Flammable, so keep away from heat sources. ..

    Article Title: Single-stranded binding proteins and helicase enhance the activity of prokaryotic argonautes in vitro
    Article Snippet: .. Materials The pMiniT™ 2.0 vector; pUC19 vector; pBR322 vector; ΦX174 Virion, RFI, and RFII DNA; Extreme-Thermostable Single-Stranded DNA Binding Protein (ET SSB); T4 Gene 32 Protein (gp32); Proteinase K; SspI-HF® ; Nt.BspQI; ThermoPol® Reaction Buffer (20 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) pH 8.8 @ 25 °C, 10 mM potassium chloride, 10 mM ammonium sulfate, 2 mM magnesium sulfate, and 0.1% Triton X-100); Taq DNA Ligase Reaction Buffer (20 mM Tris-HCl pH 7.6 @ 25 °C, 25 mM potassium acetate, 10 mM magnesium acetate, 1 mM NAD+ , 10 mM dithiothreitol (DTT), and 0.1% Triton X-100); NEBuffer™ 3.1; NEBuffer™ 4; CutSmart® Buffer; Diluent E; and Quick-Load Purple 2-Log and Low Molecular Weight (LMW) DNA Ladders were obtained from New England Biolabs, Inc. (NEB; Ipswich, Massachusetts, USA). .. Novex™ 20% polyacrylamide TBE Gels were obtained from Thermo Fisher Scientific, Inc. (Waltham, Massachusetts, USA).

    Polymerase Chain Reaction:

    Article Title: Systematic evaluation and optimization of the experimental steps in RNA G-quadruplex structure sequencing
    Article Snippet: .. PCR samples were added with 6X orange loading dye (NEB, B7022S) and then resolved on a 1.8% TAE agarose gel at 120 V, 55 min with 4 μl Quick-load Purple LMW marker (NEB, N0557S) included. .. The 150–400 nt region of each PCR product was cut and purified by using the Zymo DNA agarose gel extraction kit (Zymo, D4008) following manufacturer’s protocol.

    Binding Assay:

    Article Title: Single-stranded binding proteins and helicase enhance the activity of prokaryotic argonautes in vitro
    Article Snippet: .. Materials The pMiniT™ 2.0 vector; pUC19 vector; pBR322 vector; ΦX174 Virion, RFI, and RFII DNA; Extreme-Thermostable Single-Stranded DNA Binding Protein (ET SSB); T4 Gene 32 Protein (gp32); Proteinase K; SspI-HF® ; Nt.BspQI; ThermoPol® Reaction Buffer (20 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) pH 8.8 @ 25 °C, 10 mM potassium chloride, 10 mM ammonium sulfate, 2 mM magnesium sulfate, and 0.1% Triton X-100); Taq DNA Ligase Reaction Buffer (20 mM Tris-HCl pH 7.6 @ 25 °C, 25 mM potassium acetate, 10 mM magnesium acetate, 1 mM NAD+ , 10 mM dithiothreitol (DTT), and 0.1% Triton X-100); NEBuffer™ 3.1; NEBuffer™ 4; CutSmart® Buffer; Diluent E; and Quick-Load Purple 2-Log and Low Molecular Weight (LMW) DNA Ladders were obtained from New England Biolabs, Inc. (NEB; Ipswich, Massachusetts, USA). .. Novex™ 20% polyacrylamide TBE Gels were obtained from Thermo Fisher Scientific, Inc. (Waltham, Massachusetts, USA).

    Plasmid Preparation:

    Article Title: Single-stranded binding proteins and helicase enhance the activity of prokaryotic argonautes in vitro
    Article Snippet: .. Materials The pMiniT™ 2.0 vector; pUC19 vector; pBR322 vector; ΦX174 Virion, RFI, and RFII DNA; Extreme-Thermostable Single-Stranded DNA Binding Protein (ET SSB); T4 Gene 32 Protein (gp32); Proteinase K; SspI-HF® ; Nt.BspQI; ThermoPol® Reaction Buffer (20 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) pH 8.8 @ 25 °C, 10 mM potassium chloride, 10 mM ammonium sulfate, 2 mM magnesium sulfate, and 0.1% Triton X-100); Taq DNA Ligase Reaction Buffer (20 mM Tris-HCl pH 7.6 @ 25 °C, 25 mM potassium acetate, 10 mM magnesium acetate, 1 mM NAD+ , 10 mM dithiothreitol (DTT), and 0.1% Triton X-100); NEBuffer™ 3.1; NEBuffer™ 4; CutSmart® Buffer; Diluent E; and Quick-Load Purple 2-Log and Low Molecular Weight (LMW) DNA Ladders were obtained from New England Biolabs, Inc. (NEB; Ipswich, Massachusetts, USA). .. Novex™ 20% polyacrylamide TBE Gels were obtained from Thermo Fisher Scientific, Inc. (Waltham, Massachusetts, USA).

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  • 94
    New England Biolabs quick load purple
    Quick Load Purple, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quick load purple/product/New England Biolabs
    Average 94 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    quick load purple - by Bioz Stars, 2021-01
    94/100 stars
      Buy from Supplier

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