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Agilent technologies qubit fluorometer
Qubit Fluorometer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/qubit fluorometer/product/Agilent technologies
Average 92 stars, based on 98 article reviews
Price from $9.99 to $1999.99
qubit fluorometer - by Bioz Stars, 2020-02
92/100 stars

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Amplification:

Article Title: Hybrid Assembly of the Genome of the Entomopathogenic Nematode Steinernema carpocapsae Identifies the X-Chromosome
Article Snippet: .. After the incubation period samples were reverse transcribed and PCR amplified according to Serra et al. After PCR, samples were cleaned with Ampure XP beads, concentrations measured with Qubit fluorometer and bioanalyzed with Agilent 2100 Bioanalyzer to check for cDNA quality. .. If samples had a good BioAnalyzer profile, they were tagmented with Nextera DNA Library Prep Kit, amplified with adapters and sequenced.

Article Title: Combining Developmental and Perturbation-Seq Uncovers Transcriptional Modules Orchestrating Neuronal Remodeling
Article Snippet: Paragraph title: RNA Amplification and Library Preparation ... Prior to sequencing, libraries were evaluated by Qubit fluorometer and TapeStation (Agilent).

Article Title: Activated entomopathogenic nematode infective juveniles release lethal venom proteins
Article Snippet: Samples were spun down quickly, and amplified for 6 cycles using the PCR program with the following settings: 72°C for 5 min, 98°C for 30 s, [98°C for 10 s, 63°C for 30 s, 72°C for 1 min] for 6 cycles, 72°C for 5 min, and hold at 4°C. .. Library concentrations were measured with the Qubit fluorometer and Bioanalyzed with the 2100 Agilent Bioanalyzer.

Synthesized:

Article Title: Microbial recognition by GEF-H1 controls IKKε mediated activation of IRF5
Article Snippet: Libraries were synthesized using Illumina TruSeq Stranded mRNA sample preparation kit from 500 ng of purified total RNA and indexed adapters according to the manufacturer’s protocol (Illumina). .. The final dsDNA libraries were quantified by Qubit fluorometer, Agilent Tapestation 2200, and RT-qPCR using the Kapa Biosystems library quantification kit according to manufacturer’s protocols.

Methylated DNA Immunoprecipitation Sequencing:

Article Title: Combining MeDIP-seq and MRE-seq to investigate genome-wide CpG methylation
Article Snippet: .. Indexed MeDIP-seq libraries quantified by a Qubit fluorometer are pooled after QC and are quantified again on an Agilent 2100 Bioanalyzer. .. 101-bp paired-end sequencing is run for MeDIP-seq libraries following Illumina’s standard protocol.

Quantitative RT-PCR:

Article Title: Microbial recognition by GEF-H1 controls IKKε mediated activation of IRF5
Article Snippet: .. The final dsDNA libraries were quantified by Qubit fluorometer, Agilent Tapestation 2200, and RT-qPCR using the Kapa Biosystems library quantification kit according to manufacturer’s protocols. .. Pooled libraries were subjected to 35-bp paired-end sequencing according to the manufacturer’s protocol (Illumina NextSeq 500).

Article Title: Overcoming Resistance to Dual Innate Immune and MEK Inhibition Downstream of KRAS
Article Snippet: .. The finished dsDNA libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and qRT-PCR using the Kapa Biosystems library quantification kit according to manufacturer’s protocols. .. Uniquely indexed libraries were pooled in equimolar ratios and sequenced on an Illumina NextSeq500 with single-end 75bp reads by the Dana-Farber Cancer Institute Molecular Biology Core Facilities.

Article Title: A computationally inspired in-vivo approach identifies a link between amygdalar transcriptional heterogeneity, socialization and anxiety
Article Snippet: .. The resultant dsDNA libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and RT-qPCR using the Kapa Biosystems library quantification kit according to manufacturer’s protocols. .. Uniquely indexed libraries were pooled in equimolar ratios and sequenced on a single Illumina NextSeq500 run with single-end 75 bp reads by the Dana-Farber Cancer Institute Molecular Biology Core Facilities.

Incubation:

Article Title: Hybrid Assembly of the Genome of the Entomopathogenic Nematode Steinernema carpocapsae Identifies the X-Chromosome
Article Snippet: .. After the incubation period samples were reverse transcribed and PCR amplified according to Serra et al. After PCR, samples were cleaned with Ampure XP beads, concentrations measured with Qubit fluorometer and bioanalyzed with Agilent 2100 Bioanalyzer to check for cDNA quality. .. If samples had a good BioAnalyzer profile, they were tagmented with Nextera DNA Library Prep Kit, amplified with adapters and sequenced.

Expressing:

Article Title: H3K9 methyltransferases and demethylases control lung tumor-propagating cells and lung cancer progression
Article Snippet: The resultant dsDNA libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and quantitative reverse transcription PCR using the Kapa Biosystems Library Quantification Kit according to the manufacturer’s protocols. .. Differential gene expression testing was performed by DESeq2 (v1.10.1) and normalized read counts (RPKM) were calculated using cufflinks (v2.2.1) .

Article Title: Activated entomopathogenic nematode infective juveniles release lethal venom proteins
Article Snippet: Library concentrations were measured with the Qubit fluorometer and Bioanalyzed with the 2100 Agilent Bioanalyzer. .. Reads for single worm RNA-seq samples were submitted to Gene Expression Omnibus (GEO) under the accession number GSE89961 ( https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?token=qdcbequejxcfzef & acc=GSE89961 ).

Modification:

Article Title: Quantitative Characterization of Defective Virus Emergence by Deep Sequencing
Article Snippet: Viral RNA was submitted to the University of Wisconsin—Madison Biotechnology Center for quality control testing (Qubit Fluorometer and Agilent 2100 BioAnalyzer for quantity and integrity verification, respectively) and transcriptome sequencing (RNA-Seq) library preparation. .. A) , and the Illumina mRNA-seq 8 sample preparation kit (Illumina Inc., San Diego, CA, USA) with minimal modification.

Derivative Assay:

Article Title: Microbial recognition by GEF-H1 controls IKKε mediated activation of IRF5
Article Snippet: RNAseq and GSEA analyses Total RNA was isolated from BMDMs derived from WT, Arhgef2 −/− , and Irf5 −/− mice, using RNeasy Micro kit (Qiagen). .. The final dsDNA libraries were quantified by Qubit fluorometer, Agilent Tapestation 2200, and RT-qPCR using the Kapa Biosystems library quantification kit according to manufacturer’s protocols.

Ligation:

Article Title: Combining Developmental and Perturbation-Seq Uncovers Transcriptional Modules Orchestrating Neuronal Remodeling
Article Snippet: The resulting RNA was fragmented and converted into an Illumina sequencing-ready library through ligation, RT, and PCR. .. Prior to sequencing, libraries were evaluated by Qubit fluorometer and TapeStation (Agilent).

Biomarker Assay:

Article Title: Integrated mRNA, sRNA, and degradome sequencing reveal oilseed rape complex responses to Sclerotinia sclerotiorum (Lib.) infection
Article Snippet: A Qubit Fluorometer was used to determine the RNA quantity and quantity and an Agilent 2100 Bioanalyzer was used to detect the RNA purity and integrity. .. Two RNA samples were sent to Beijing Biomarker Technologies Co., Ltd. (Beijing, China) for mRNA, sRNA, and degradome sequencing using an Illumina HiSeqTM 2500.

Cell Culture:

Article Title: Overcoming Resistance to Dual Innate Immune and MEK Inhibition Downstream of KRAS
Article Snippet: RNA extraction was performed using RNeasy Mini Kit (Qiagen, Cat.# 74106) and PerfectPure RNA cultured cell Enzyme Set DNase (5 PRIME, Cat.# 2900315). .. The finished dsDNA libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and qRT-PCR using the Kapa Biosystems library quantification kit according to manufacturer’s protocols.

Generated:

Article Title: Quantitative Characterization of Defective Virus Emergence by Deep Sequencing
Article Snippet: Viral RNA was submitted to the University of Wisconsin—Madison Biotechnology Center for quality control testing (Qubit Fluorometer and Agilent 2100 BioAnalyzer for quantity and integrity verification, respectively) and transcriptome sequencing (RNA-Seq) library preparation. .. Each library was generated using Illumina's guide, Preparing Samples for Sequencing of mRNA (Rev.

Article Title: Transcriptome Differences in Porcine Alveolar Macrophages from Tongcheng and Large White Pigs in Response to Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Infection
Article Snippet: RNA Preparation and Sequencing RNA degradation and contamination was monitored on 1% agarose gels and RNA purity, concentration, and integrity were measured by Nanodrop, Qubit Fluorometer and Agilent 2100 Bioanalyzer. .. After library preparation and quality control, the library preparations were sequenced on an Illumina Hiseq 2000 platform and 100 bp paired-end reads were generated.

Sequencing:

Article Title: Integrated mRNA, sRNA, and degradome sequencing reveal oilseed rape complex responses to Sclerotinia sclerotiorum (Lib.) infection
Article Snippet: Paragraph title: RNA isolation and high-throughput sequencing ... A Qubit Fluorometer was used to determine the RNA quantity and quantity and an Agilent 2100 Bioanalyzer was used to detect the RNA purity and integrity.

Article Title: Microbial recognition by GEF-H1 controls IKKε mediated activation of IRF5
Article Snippet: The final dsDNA libraries were quantified by Qubit fluorometer, Agilent Tapestation 2200, and RT-qPCR using the Kapa Biosystems library quantification kit according to manufacturer’s protocols. .. Pooled libraries were subjected to 35-bp paired-end sequencing according to the manufacturer’s protocol (Illumina NextSeq 500).

Article Title: Hybrid Assembly of the Genome of the Entomopathogenic Nematode Steinernema carpocapsae Identifies the X-Chromosome
Article Snippet: Then, S. carpocapsae single nematode transcriptome sequencing was performed according to . .. After the incubation period samples were reverse transcribed and PCR amplified according to Serra et al. After PCR, samples were cleaned with Ampure XP beads, concentrations measured with Qubit fluorometer and bioanalyzed with Agilent 2100 Bioanalyzer to check for cDNA quality.

Article Title: Quantitative Characterization of Defective Virus Emergence by Deep Sequencing
Article Snippet: .. Viral RNA was submitted to the University of Wisconsin—Madison Biotechnology Center for quality control testing (Qubit Fluorometer and Agilent 2100 BioAnalyzer for quantity and integrity verification, respectively) and transcriptome sequencing (RNA-Seq) library preparation. .. Each library was generated using Illumina's guide, Preparing Samples for Sequencing of mRNA (Rev.

Article Title: Identification of Novel and Conserved miRNAs from Extreme Halophyte, Oryza coarctata, a Wild Relative of Rice
Article Snippet: Paragraph title: Extraction and sequencing of small RNAs ... The prepared library was quantified by using Qubit Fluorometer, and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip (Agilent).

Article Title: Combining Developmental and Perturbation-Seq Uncovers Transcriptional Modules Orchestrating Neuronal Remodeling
Article Snippet: .. Prior to sequencing, libraries were evaluated by Qubit fluorometer and TapeStation (Agilent). .. Analysis of RNA-Seq Data The samples were sequenced using Illumina NextSeq 500 sequencer, at a sequencing depth of an average of 3.5 and 5 million reads per sample for the developmental-seq and perturbations-seq, respectively.

Article Title: Combining MeDIP-seq and MRE-seq to investigate genome-wide CpG methylation
Article Snippet: Indexed MeDIP-seq libraries quantified by a Qubit fluorometer are pooled after QC and are quantified again on an Agilent 2100 Bioanalyzer. .. 101-bp paired-end sequencing is run for MeDIP-seq libraries following Illumina’s standard protocol.

Article Title: Transcriptome Differences in Porcine Alveolar Macrophages from Tongcheng and Large White Pigs in Response to Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Infection
Article Snippet: .. RNA Preparation and Sequencing RNA degradation and contamination was monitored on 1% agarose gels and RNA purity, concentration, and integrity were measured by Nanodrop, Qubit Fluorometer and Agilent 2100 Bioanalyzer. .. The total RNA of PAMs from 12 selected pigs (3 pigs selected randomly from each group) were used for sequencing with NEBNext® UltraTM RNA Library Prep kit for Illumina® (NEB, Ipswich, MA, USA), and all the procedures and standards were performed following the manufacturer’s protocols.

Article Title: Activated entomopathogenic nematode infective juveniles release lethal venom proteins
Article Snippet: Paragraph title: Single nematode transcriptome sequencing ... Library concentrations were measured with the Qubit fluorometer and Bioanalyzed with the 2100 Agilent Bioanalyzer.

Article Title: A computationally inspired in-vivo approach identifies a link between amygdalar transcriptional heterogeneity, socialization and anxiety
Article Snippet: Paragraph title: RNA Library preparation and sequencing ... The resultant dsDNA libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and RT-qPCR using the Kapa Biosystems library quantification kit according to manufacturer’s protocols.

Article Title: Individual Apostichopus japonicus fecal microbiome reveals a link with polyhydroxybutyrate producers in host growth gaps
Article Snippet: Paragraph title: Metagenomic sequencing ... The DNA quality and quantity were estimated using Nanodrop, Qubit Fluorometer and Agilent 2200 TapeStation System.

RNA Sequencing Assay:

Article Title: SREBP1 drives Keratin-80-dependent cytoskeletal changes and invasive behavior in endocrine-resistant ERα breast cancer
Article Snippet: .. RNA sequencing and single cell RNA-seq Total RNA from each sample was quantified by Qubit® Fluorometer and quality checked by Agilent Bioanalyzer® RNA 6000 Nano Chip. ..

Article Title: Hybrid Assembly of the Genome of the Entomopathogenic Nematode Steinernema carpocapsae Identifies the X-Chromosome
Article Snippet: Paragraph title: RNA-sequencing of S. carpocapsae time-course, male, and female ... After the incubation period samples were reverse transcribed and PCR amplified according to Serra et al. After PCR, samples were cleaned with Ampure XP beads, concentrations measured with Qubit fluorometer and bioanalyzed with Agilent 2100 Bioanalyzer to check for cDNA quality.

Article Title: Quantitative Characterization of Defective Virus Emergence by Deep Sequencing
Article Snippet: .. Viral RNA was submitted to the University of Wisconsin—Madison Biotechnology Center for quality control testing (Qubit Fluorometer and Agilent 2100 BioAnalyzer for quantity and integrity verification, respectively) and transcriptome sequencing (RNA-Seq) library preparation. .. Each library was generated using Illumina's guide, Preparing Samples for Sequencing of mRNA (Rev.

Article Title: H3K9 methyltransferases and demethylases control lung tumor-propagating cells and lung cancer progression
Article Snippet: Paragraph title: RNA-sequencing ... The resultant dsDNA libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and quantitative reverse transcription PCR using the Kapa Biosystems Library Quantification Kit according to the manufacturer’s protocols.

Article Title: Transcriptome profiles in peripheral white blood cells at the time of artificial insemination discriminate beef heifers with different fertility potential
Article Snippet: Paragraph title: RNA extraction, library preparation, and RNA sequencing ... RNA yield was quantified using the Qubit™ RNA Broad Range Assay Kit (Eurogene, OR) on a Qubit® Fluorometer, and integrity was assessed on Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA) using an Agilent RNA 6000 Nano kit (Agilent, Santa Clara, CA).

Article Title: Activated entomopathogenic nematode infective juveniles release lethal venom proteins
Article Snippet: Library concentrations were measured with the Qubit fluorometer and Bioanalyzed with the 2100 Agilent Bioanalyzer. .. Reads for single worm RNA-seq samples were submitted to Gene Expression Omnibus (GEO) under the accession number GSE89961 ( https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?token=qdcbequejxcfzef & acc=GSE89961 ).

Article Title: Overcoming Resistance to Dual Innate Immune and MEK Inhibition Downstream of KRAS
Article Snippet: Paragraph title: RNA Preparation and RNA-seq ... The finished dsDNA libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and qRT-PCR using the Kapa Biosystems library quantification kit according to manufacturer’s protocols.

Isolation:

Article Title: Integrated mRNA, sRNA, and degradome sequencing reveal oilseed rape complex responses to Sclerotinia sclerotiorum (Lib.) infection
Article Snippet: Paragraph title: RNA isolation and high-throughput sequencing ... A Qubit Fluorometer was used to determine the RNA quantity and quantity and an Agilent 2100 Bioanalyzer was used to detect the RNA purity and integrity.

Article Title: Microbial recognition by GEF-H1 controls IKKε mediated activation of IRF5
Article Snippet: RNAseq and GSEA analyses Total RNA was isolated from BMDMs derived from WT, Arhgef2 −/− , and Irf5 −/− mice, using RNeasy Micro kit (Qiagen). .. The final dsDNA libraries were quantified by Qubit fluorometer, Agilent Tapestation 2200, and RT-qPCR using the Kapa Biosystems library quantification kit according to manufacturer’s protocols.

Article Title: Transcriptome profiles in peripheral white blood cells at the time of artificial insemination discriminate beef heifers with different fertility potential
Article Snippet: Total RNA was then isolated from PWBCs of 23 heifers using TRIzol™ reagent (Invitrogen, Carlsbad, CA) following the manufacturer’s protocol. .. RNA yield was quantified using the Qubit™ RNA Broad Range Assay Kit (Eurogene, OR) on a Qubit® Fluorometer, and integrity was assessed on Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA) using an Agilent RNA 6000 Nano kit (Agilent, Santa Clara, CA).

Purification:

Article Title: Microbial recognition by GEF-H1 controls IKKε mediated activation of IRF5
Article Snippet: Libraries were synthesized using Illumina TruSeq Stranded mRNA sample preparation kit from 500 ng of purified total RNA and indexed adapters according to the manufacturer’s protocol (Illumina). .. The final dsDNA libraries were quantified by Qubit fluorometer, Agilent Tapestation 2200, and RT-qPCR using the Kapa Biosystems library quantification kit according to manufacturer’s protocols.

Article Title: Identification of Novel and Conserved miRNAs from Extreme Halophyte, Oryza coarctata, a Wild Relative of Rice
Article Snippet: Small RNAs of 16–36 nt in length were purified from a 15% denaturing poly-acrylamide gel and then, 5’ and 3’ adaptors were ligated sequentially to the small RNAs. .. The prepared library was quantified by using Qubit Fluorometer, and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip (Agilent).

Article Title: H3K9 methyltransferases and demethylases control lung tumor-propagating cells and lung cancer progression
Article Snippet: RNA libraries were prepared using Illumina TruSeq Stranded mRNA Sample Preparation Kits from 500 ng of purified total RNA according to the manufacturer’s protocol. .. The resultant dsDNA libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and quantitative reverse transcription PCR using the Kapa Biosystems Library Quantification Kit according to the manufacturer’s protocols.

Article Title: Overcoming Resistance to Dual Innate Immune and MEK Inhibition Downstream of KRAS
Article Snippet: Libraries were prepared using Illumina TruSeq Stranded mRNA sample preparation kits from 500 ng of purified total RNA according to the manufacturer’s protocol. .. The finished dsDNA libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and qRT-PCR using the Kapa Biosystems library quantification kit according to manufacturer’s protocols.

Article Title: A computationally inspired in-vivo approach identifies a link between amygdalar transcriptional heterogeneity, socialization and anxiety
Article Snippet: RNA Library preparation and sequencing RNA libraries were prepared using Illumina TruSeq Stranded mRNA sample preparation kits from 500 ng of purified total RNA according to the manufacturer’s protocol. .. The resultant dsDNA libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and RT-qPCR using the Kapa Biosystems library quantification kit according to manufacturer’s protocols.

Polymerase Chain Reaction:

Article Title: Hybrid Assembly of the Genome of the Entomopathogenic Nematode Steinernema carpocapsae Identifies the X-Chromosome
Article Snippet: .. After the incubation period samples were reverse transcribed and PCR amplified according to Serra et al. After PCR, samples were cleaned with Ampure XP beads, concentrations measured with Qubit fluorometer and bioanalyzed with Agilent 2100 Bioanalyzer to check for cDNA quality. .. If samples had a good BioAnalyzer profile, they were tagmented with Nextera DNA Library Prep Kit, amplified with adapters and sequenced.

Article Title: Identification of Novel and Conserved miRNAs from Extreme Halophyte, Oryza coarctata, a Wild Relative of Rice
Article Snippet: Ligated products were reverse transcribed by superscript III Reverse transcriptase (Invitrogen, USA) and resultant cDNA was enriched by PCR (15 cycles) and cleaned by using polyacrylamide gel. .. The prepared library was quantified by using Qubit Fluorometer, and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip (Agilent).

Article Title: Combining Developmental and Perturbation-Seq Uncovers Transcriptional Modules Orchestrating Neuronal Remodeling
Article Snippet: The resulting RNA was fragmented and converted into an Illumina sequencing-ready library through ligation, RT, and PCR. .. Prior to sequencing, libraries were evaluated by Qubit fluorometer and TapeStation (Agilent).

Article Title: H3K9 methyltransferases and demethylases control lung tumor-propagating cells and lung cancer progression
Article Snippet: .. The resultant dsDNA libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and quantitative reverse transcription PCR using the Kapa Biosystems Library Quantification Kit according to the manufacturer’s protocols. .. Uniquely indexed libraries were pooled in equimolar ratios and sequenced on a single Illumina NextSeq500 run with single-end 75 bp reads by the Dana-Farber Cancer Institute Molecular Biology Core Facilities.

Article Title: Activated entomopathogenic nematode infective juveniles release lethal venom proteins
Article Snippet: PCR primers were cleaned from the sample by mixing the sample with a 1:1 ratio of Ampure XP beads. .. Library concentrations were measured with the Qubit fluorometer and Bioanalyzed with the 2100 Agilent Bioanalyzer.

Construct:

Article Title: Identification of Novel and Conserved miRNAs from Extreme Halophyte, Oryza coarctata, a Wild Relative of Rice
Article Snippet: Small RNA libraries for sequencing were constructed according to the Illumina protocol, outlined in “TruSeq Small RNA Sample Preparation Guide”. .. The prepared library was quantified by using Qubit Fluorometer, and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip (Agilent).

Mouse Assay:

Article Title: Microbial recognition by GEF-H1 controls IKKε mediated activation of IRF5
Article Snippet: RNAseq and GSEA analyses Total RNA was isolated from BMDMs derived from WT, Arhgef2 −/− , and Irf5 −/− mice, using RNeasy Micro kit (Qiagen). .. The final dsDNA libraries were quantified by Qubit fluorometer, Agilent Tapestation 2200, and RT-qPCR using the Kapa Biosystems library quantification kit according to manufacturer’s protocols.

Chromatin Immunoprecipitation:

Article Title: SREBP1 drives Keratin-80-dependent cytoskeletal changes and invasive behavior in endocrine-resistant ERα breast cancer
Article Snippet: .. RNA sequencing and single cell RNA-seq Total RNA from each sample was quantified by Qubit® Fluorometer and quality checked by Agilent Bioanalyzer® RNA 6000 Nano Chip. ..

Article Title: Identification of Novel and Conserved miRNAs from Extreme Halophyte, Oryza coarctata, a Wild Relative of Rice
Article Snippet: .. The prepared library was quantified by using Qubit Fluorometer, and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip (Agilent). .. All sequence data have been submitted to NCBI ( http://www.ncbi.nlm.nih.gov/ ) as Bioproject (PRJNA271658), Biosample (SAMN03275735), and Sequence Read Archive (SRA) data base under the submission accession number SRX831195.

Software:

Article Title: Microbial recognition by GEF-H1 controls IKKε mediated activation of IRF5
Article Snippet: The final dsDNA libraries were quantified by Qubit fluorometer, Agilent Tapestation 2200, and RT-qPCR using the Kapa Biosystems library quantification kit according to manufacturer’s protocols. .. Blc2fastq2 Conversion software (Illumina) was used to generate de-multiplexed Fastq files.

RNA Extraction:

Article Title: Transcriptome profiles in peripheral white blood cells at the time of artificial insemination discriminate beef heifers with different fertility potential
Article Snippet: Paragraph title: RNA extraction, library preparation, and RNA sequencing ... RNA yield was quantified using the Qubit™ RNA Broad Range Assay Kit (Eurogene, OR) on a Qubit® Fluorometer, and integrity was assessed on Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA) using an Agilent RNA 6000 Nano kit (Agilent, Santa Clara, CA).

Article Title: Overcoming Resistance to Dual Innate Immune and MEK Inhibition Downstream of KRAS
Article Snippet: RNA extraction was performed using RNeasy Mini Kit (Qiagen, Cat.# 74106) and PerfectPure RNA cultured cell Enzyme Set DNase (5 PRIME, Cat.# 2900315). .. The finished dsDNA libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and qRT-PCR using the Kapa Biosystems library quantification kit according to manufacturer’s protocols.

Sample Prep:

Article Title: Microbial recognition by GEF-H1 controls IKKε mediated activation of IRF5
Article Snippet: Libraries were synthesized using Illumina TruSeq Stranded mRNA sample preparation kit from 500 ng of purified total RNA and indexed adapters according to the manufacturer’s protocol (Illumina). .. The final dsDNA libraries were quantified by Qubit fluorometer, Agilent Tapestation 2200, and RT-qPCR using the Kapa Biosystems library quantification kit according to manufacturer’s protocols.

Article Title: Quantitative Characterization of Defective Virus Emergence by Deep Sequencing
Article Snippet: Viral RNA was submitted to the University of Wisconsin—Madison Biotechnology Center for quality control testing (Qubit Fluorometer and Agilent 2100 BioAnalyzer for quantity and integrity verification, respectively) and transcriptome sequencing (RNA-Seq) library preparation. .. A) , and the Illumina mRNA-seq 8 sample preparation kit (Illumina Inc., San Diego, CA, USA) with minimal modification.

Article Title: Identification of Novel and Conserved miRNAs from Extreme Halophyte, Oryza coarctata, a Wild Relative of Rice
Article Snippet: Small RNA libraries for sequencing were constructed according to the Illumina protocol, outlined in “TruSeq Small RNA Sample Preparation Guide”. .. The prepared library was quantified by using Qubit Fluorometer, and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip (Agilent).

Article Title: H3K9 methyltransferases and demethylases control lung tumor-propagating cells and lung cancer progression
Article Snippet: RNA libraries were prepared using Illumina TruSeq Stranded mRNA Sample Preparation Kits from 500 ng of purified total RNA according to the manufacturer’s protocol. .. The resultant dsDNA libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and quantitative reverse transcription PCR using the Kapa Biosystems Library Quantification Kit according to the manufacturer’s protocols.

Article Title: Overcoming Resistance to Dual Innate Immune and MEK Inhibition Downstream of KRAS
Article Snippet: Libraries were prepared using Illumina TruSeq Stranded mRNA sample preparation kits from 500 ng of purified total RNA according to the manufacturer’s protocol. .. The finished dsDNA libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and qRT-PCR using the Kapa Biosystems library quantification kit according to manufacturer’s protocols.

Article Title: A computationally inspired in-vivo approach identifies a link between amygdalar transcriptional heterogeneity, socialization and anxiety
Article Snippet: RNA Library preparation and sequencing RNA libraries were prepared using Illumina TruSeq Stranded mRNA sample preparation kits from 500 ng of purified total RNA according to the manufacturer’s protocol. .. The resultant dsDNA libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and RT-qPCR using the Kapa Biosystems library quantification kit according to manufacturer’s protocols.

Article Title: Individual Apostichopus japonicus fecal microbiome reveals a link with polyhydroxybutyrate producers in host growth gaps
Article Snippet: The DNA quality and quantity were estimated using Nanodrop, Qubit Fluorometer and Agilent 2200 TapeStation System. .. By using TruSeq Nano DNA LT Sample Prep Kit, genomic DNA was fragment and insert DNA of 350 bp was selected and connected adapter sequences.

In Vitro:

Article Title: Combining Developmental and Perturbation-Seq Uncovers Transcriptional Modules Orchestrating Neuronal Remodeling
Article Snippet: In brief, mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. .. Prior to sequencing, libraries were evaluated by Qubit fluorometer and TapeStation (Agilent).

Next-Generation Sequencing:

Article Title: Combining MeDIP-seq and MRE-seq to investigate genome-wide CpG methylation
Article Snippet: Paragraph title: 3.1.8. Next-generation sequencing ... Indexed MeDIP-seq libraries quantified by a Qubit fluorometer are pooled after QC and are quantified again on an Agilent 2100 Bioanalyzer.

Concentration Assay:

Article Title: Transcriptome Differences in Porcine Alveolar Macrophages from Tongcheng and Large White Pigs in Response to Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Infection
Article Snippet: .. RNA Preparation and Sequencing RNA degradation and contamination was monitored on 1% agarose gels and RNA purity, concentration, and integrity were measured by Nanodrop, Qubit Fluorometer and Agilent 2100 Bioanalyzer. .. The total RNA of PAMs from 12 selected pigs (3 pigs selected randomly from each group) were used for sequencing with NEBNext® UltraTM RNA Library Prep kit for Illumina® (NEB, Ipswich, MA, USA), and all the procedures and standards were performed following the manufacturer’s protocols.

High Throughput Screening Assay:

Article Title: Integrated mRNA, sRNA, and degradome sequencing reveal oilseed rape complex responses to Sclerotinia sclerotiorum (Lib.) infection
Article Snippet: Paragraph title: RNA isolation and high-throughput sequencing ... A Qubit Fluorometer was used to determine the RNA quantity and quantity and an Agilent 2100 Bioanalyzer was used to detect the RNA purity and integrity.

Lysis:

Article Title: Hybrid Assembly of the Genome of the Entomopathogenic Nematode Steinernema carpocapsae Identifies the X-Chromosome
Article Snippet: Then, 2 µl of lysis buffer (for recipe see ) which has RNase inhibitor and proteinase K was added to the tube and incubated at 65° for 10min, 85° for 1min. .. After the incubation period samples were reverse transcribed and PCR amplified according to Serra et al. After PCR, samples were cleaned with Ampure XP beads, concentrations measured with Qubit fluorometer and bioanalyzed with Agilent 2100 Bioanalyzer to check for cDNA quality.

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    Agilent technologies qubit fluorometer tfs
    Qubit Fluorometer Tfs, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorometer tfs/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qubit fluorometer tfs - by Bioz Stars, 2020-02
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    Agilent technologies qubit fluorometer
    Qubit Fluorometer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorometer/product/Agilent technologies
    Average 92 stars, based on 100 article reviews
    Price from $9.99 to $1999.99
    qubit fluorometer - by Bioz Stars, 2020-02
    92/100 stars
      Buy from Supplier

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