qubit 2 0 fluorometer rna br hs assay kit  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Name:
    Qubit RNA HS Assay Kit
    Description:
    The Qubit RNA HS Assay Kit when used with the Qubit Fluorometer provides an accurate and selective method for the quantitation of low abundance RNA samples The assay is highly selective for RNA and will not quantitate DNA protein or free nucleotides Common contaminants such as salts free nucleotides solvents detergents or protein are well tolerated in the assay The assay kit is designed to be accurate for RNA sample concentrations between 250 pg µL and 100 ng µL The kit provides concentrated assay reagent dilution buffer and pre diluted RNA standards Simply dilute the reagent using the buffer provided add your sample any volume between 1 µL and 20 µL is acceptable and read the concentration using the Qubit Fluorometer Which product to choose for RNA HS High Sensitivity quantitation • For 1 20 samples use this Qubit RNA HS Assay Kit with the Qubit Fluorometer • For 20 2000 samples use the Quant iT RNA HS Assay Kit with microplate readerNotes 1 All Qubit assay kits can be used with the Qubit 1 0 Qubit 2 0 Qubit 3 and Qubit 4 fluorometers 2 500 µL thin walled PCR tubes are required but not included
    Catalog Number:
    q32852
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|RNA Quantitation|Nucleic Acid Quantitation
    Category:
    Kits and Assays
    Buy from Supplier


    Structured Review

    Thermo Fisher qubit 2 0 fluorometer rna br hs assay kit
    The Qubit RNA HS Assay Kit when used with the Qubit Fluorometer provides an accurate and selective method for the quantitation of low abundance RNA samples The assay is highly selective for RNA and will not quantitate DNA protein or free nucleotides Common contaminants such as salts free nucleotides solvents detergents or protein are well tolerated in the assay The assay kit is designed to be accurate for RNA sample concentrations between 250 pg µL and 100 ng µL The kit provides concentrated assay reagent dilution buffer and pre diluted RNA standards Simply dilute the reagent using the buffer provided add your sample any volume between 1 µL and 20 µL is acceptable and read the concentration using the Qubit Fluorometer Which product to choose for RNA HS High Sensitivity quantitation • For 1 20 samples use this Qubit RNA HS Assay Kit with the Qubit Fluorometer • For 20 2000 samples use the Quant iT RNA HS Assay Kit with microplate readerNotes 1 All Qubit assay kits can be used with the Qubit 1 0 Qubit 2 0 Qubit 3 and Qubit 4 fluorometers 2 500 µL thin walled PCR tubes are required but not included
    https://www.bioz.com/result/qubit 2 0 fluorometer rna br hs assay kit/product/Thermo Fisher
    Average 90 stars, based on 866 article reviews
    Price from $9.99 to $1999.99
    qubit 2 0 fluorometer rna br hs assay kit - by Bioz Stars, 2020-01
    90/100 stars

    Images

    Related Articles

    Centrifugation:

    Article Title: Cultivation and Transcriptional Analysis of a Canonical Nitrospira Under Stable Growth Conditions
    Article Snippet: Biomass of 70 ml culture volume was harvested by centrifugation at 20,000 × g , 4°C, 10–20 min. RNA was immediately extracted using TRIzol reagent (Thermo Fisher Scientific) following the manufacturer’s protocol with the following modifications: all incubation and centrifugation times were doubled, and RNA was precipitated overnight at -20°C, with subsequent centrifugation at 12,000 × g , 4°C, 20–30 min. No heating step for RNA resuspension was included. .. RNA quantity was controlled using the Qubit RNA HS Assay Kit (Thermo Fisher Scientific).

    Amplification:

    Article Title: Transcriptional Landscape of a blaKPC-2 Plasmid and Response to Imipenem Exposure in Escherichia coli TOP10
    Article Snippet: Verification of complete removal of any contaminating DNA was performed via PCR amplification of recA housekeeping gene. .. Ribodepleted RNA concentration was measured using Qubit™ RNA HS assay kit (Invitrogen).

    Article Title: RNA-sequence data normalization through in silico prediction of reference genes: the bacterial response to DNA damage as case study
    Article Snippet: qRT-PCR RNA concentrations were determined with a Qubit 2.0 Fluorometer (Invitrogen) using the Qubit RNA HS Assay Kit (Molecular Probes). .. The Brilliant III Ultra-Fast SYBR Green QRT-PCR Master Mix (Agilent Technologies) was applied to perform an ultra-fast one-step protocol on a StepOnePlus Real-Time PCR System (Applied Biosystems/Life Technologies) using the following settings for amplification: 50 °C - 10 min, 95 °C - 3 min, 45× (95 °C - 5 s, 60 °C - 10 s).

    Mass Spectrometry:

    Article Title: Transcriptome analysis of the adult human Klinefelter testis and cellularity-matched controls reveals disturbed differentiation of Sertoli- and Leydig cells
    Article Snippet: RNA extraction Total RNA was extracted using the Recover All Total Nucleic Acid Isolation Kit for FFPE (Thermo Fisher Scientific, MS, USA) according to the manufacturer’s recommendations. .. To assess the quantity and quality of the samples, the Qubit RNA HS Assay Kit (Thermo Fisher Scientific) and the Agilent RNA 6000 Pico Kit (Agilent Technologies, CA, USA), respectively, were used.

    Construct:

    Article Title: Datasets for next-generation sequencing of DNA and RNA from urine and plasma of patients with prostate cancer
    Article Snippet: RNA concentration was determined by fluorometer Qubit 2.0 using Qubit RNA HS Assay Kit (Thermo Fisher). .. Transcriptomic libraries were constructed using Ion Total RNA-Seq Kit v2 (Life Technologies) with the following modifications of the protocol.

    Real-time Polymerase Chain Reaction:

    Article Title: Cellular and Molecular Responses Unique to Major Injury Are Dispensable for Planarian Regeneration
    Article Snippet: Paragraph title: Quantitative real-time PCR (qRT-PCR) ... Following RNA purification and resuspension in MilliQ H2 O, concentrations for each sample were determined using the Qubit RNA HS Assay Kit ( Life Technologies ).

    Article Title: The Role of Circulating MicroRNA in the Regulation of Beta Cell Function and Insulin Resistance among Indians with Type 2 Diabetes
    Article Snippet: Paragraph title: miRNA extraction and quantitative PCR ... Quantitation of the small RNA was done using the Qubit Hs RNA assay reagents (Invitrogen #Q32852-Qubit Hs RNA assay kit) on a fluorimeter (Qubit® 2.0 Fluorometer Cat# Q32866). miRNA was converted to cDNA using stem loop primers specific for the chosen miRNA using Taqman MicroRNA Reverse Transcription Kit (Applied Biosystems, #4366596) according to published protocols.

    Article Title: RNA-sequence data normalization through in silico prediction of reference genes: the bacterial response to DNA damage as case study
    Article Snippet: qRT-PCR RNA concentrations were determined with a Qubit 2.0 Fluorometer (Invitrogen) using the Qubit RNA HS Assay Kit (Molecular Probes). .. The Brilliant III Ultra-Fast SYBR Green QRT-PCR Master Mix (Agilent Technologies) was applied to perform an ultra-fast one-step protocol on a StepOnePlus Real-Time PCR System (Applied Biosystems/Life Technologies) using the following settings for amplification: 50 °C - 10 min, 95 °C - 3 min, 45× (95 °C - 5 s, 60 °C - 10 s).

    Incubation:

    Article Title: Cultivation and Transcriptional Analysis of a Canonical Nitrospira Under Stable Growth Conditions
    Article Snippet: Biomass of 70 ml culture volume was harvested by centrifugation at 20,000 × g , 4°C, 10–20 min. RNA was immediately extracted using TRIzol reagent (Thermo Fisher Scientific) following the manufacturer’s protocol with the following modifications: all incubation and centrifugation times were doubled, and RNA was precipitated overnight at -20°C, with subsequent centrifugation at 12,000 × g , 4°C, 20–30 min. No heating step for RNA resuspension was included. .. RNA quantity was controlled using the Qubit RNA HS Assay Kit (Thermo Fisher Scientific).

    Article Title: Datasets for next-generation sequencing of DNA and RNA from urine and plasma of patients with prostate cancer
    Article Snippet: RNA concentration was determined by fluorometer Qubit 2.0 using Qubit RNA HS Assay Kit (Thermo Fisher). .. After that 1 µl of 10 µM ATP and 1 µl of polynucleotide kinase (Fermentas) were added to the solution from the previous step and the whole mix was incubated at 37 °С for 30 min. Fragmented RNA was cleaned up using Micro Bio-Spin Chromatography Columns (Bio-Rad).

    Formalin-fixed Paraffin-Embedded:

    Article Title: Robust detection of immune transcripts in FFPE samples using targeted RNA sequencing
    Article Snippet: The truXTRAC™ FFPE RNA Kit is designed for use with the Adaptive Focused Acoustics AFA™ process. .. RNA was quantified by means of the Qubit RNA HS Assay Kit (Thermo Fisher Scientific).

    Article Title: Transcriptome analysis of the adult human Klinefelter testis and cellularity-matched controls reveals disturbed differentiation of Sertoli- and Leydig cells
    Article Snippet: RNA extraction Total RNA was extracted using the Recover All Total Nucleic Acid Isolation Kit for FFPE (Thermo Fisher Scientific, MS, USA) according to the manufacturer’s recommendations. .. To assess the quantity and quality of the samples, the Qubit RNA HS Assay Kit (Thermo Fisher Scientific) and the Agilent RNA 6000 Pico Kit (Agilent Technologies, CA, USA), respectively, were used.

    Expressing:

    Article Title: Enhanced protection of C57 BL/6 vs Balb/c mice to melanoma liver metastasis is mediated by NK cells
    Article Snippet: The concentration and quality of the RNA was measured with the Qubit® RNA HS Assay Kit (Thermo Fisher Scientific) and the RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). .. RNA reads were aligned to the mm9 reference genome using STAR aligner, and gene expression levels were determined by counting reads overlapping transcript exons and junctions and normalizing to RPKM expression units (reads which map per kilobase of transcript length per million mapped reads).

    Article Title: Cellular and Molecular Responses Unique to Major Injury Are Dispensable for Planarian Regeneration
    Article Snippet: Following RNA purification and resuspension in MilliQ H2 O, concentrations for each sample were determined using the Qubit RNA HS Assay Kit ( Life Technologies ). .. Relative expression levels were determined by the -ΔΔCt method by calculating the difference from the average ΔCt value of control RNAi replicates.

    Modification:

    Article Title: Tubulin C-terminal Post-translational Modifications Do Not Occur in Wood Forming Tissue of Populus
    Article Snippet: RNA-Seq Analysis The total RNA was extracted with the Direct-zol RNA Kit (Zymo Research) using Plant RNA Reagent (Life Technologies) and quantified with the Qubit RNA HS Assay Kit on a Qubit fluorometer (Life Technologies). .. After filtering to remove rRNA sequences, data were processed by a local assembly pipeline (Gu and Tsai, unpublished) modified from for de novo assembly of TUA and TUB sequences.

    Chromatography:

    Article Title: Datasets for next-generation sequencing of DNA and RNA from urine and plasma of patients with prostate cancer
    Article Snippet: RNA concentration was determined by fluorometer Qubit 2.0 using Qubit RNA HS Assay Kit (Thermo Fisher). .. After that 1 µl of 10 µM ATP and 1 µl of polynucleotide kinase (Fermentas) were added to the solution from the previous step and the whole mix was incubated at 37 °С for 30 min. Fragmented RNA was cleaned up using Micro Bio-Spin Chromatography Columns (Bio-Rad).

    Light Microscopy:

    Article Title: Comparative Genomics of Completely Sequenced Lactobacillus helveticus Genomes Provides Insights into Strain-Specific Genes and Resolves Metagenomics Data Down to the Strain Level
    Article Snippet: L. helveticus strains FAM8105, FAM22155, and FAM8627 were obtained from the Agroscope culture collection (Agroscope, Liebefeld, Switzerland) and grown under aerobic conditions in 10 mL MRS broth (De Man et al., ) at 37°C overnight (see Supplementary Figure for light microscopy images). .. The gDNA purity was assessed by controlling for RNA contamination using the Qubit RNA HS Assay kit (Thermo Scientific, Massachusetts, USA).

    Generated:

    Article Title: De novo assembly of Aureococcus anophagefferens transcriptomes reveals diverse responses to the low nutrient and low light conditions present during blooms
    Article Snippet: RNA library preparation and sequencing Samples were quantified using Invitrogen Qubit Q32855 and RNA quality was assessed using the Agilent 2100 Bioanalyzer. .. Over 2 Gbp of sequence was generated per library.

    Article Title: Gene expression in the mixotrophic prymnesiophyte, Prymnesium parvum, responds to prey availability
    Article Snippet: RNA was quantified again at the sequencing center using Invitrogen Qubit Q32855, and RNA quality was assessed using the Agilent 2100 Bioanalyzer. cDNA libraries were made from 2 μg RNA using Illumina's TruSeq RNA Sample Preparation Kit. .. Libraries were sequenced on an Illumina HiSeq 2000 which generated 2 × 50 bp (paired-end) reads.

    Sequencing:

    Article Title: Cultivation and Transcriptional Analysis of a Canonical Nitrospira Under Stable Growth Conditions
    Article Snippet: Paragraph title: RNA Extraction and Transcriptome Sequencing ... RNA quantity was controlled using the Qubit RNA HS Assay Kit (Thermo Fisher Scientific).

    Article Title: Comparative Transcriptome Analysis of Four Prymnesiophyte Algae
    Article Snippet: .. Library Preparation and Sequencing All samples were quantified again at the sequencing center using Invitrogen Qubit Q32855 and RNA quality was assessed using the Agilent 2100 Bioanalyzer. .. Libraries were made from 2 µg RNA using Illumina's TruSeq RNA Sample Preparation Kit.

    Article Title: De novo assembly of Aureococcus anophagefferens transcriptomes reveals diverse responses to the low nutrient and low light conditions present during blooms
    Article Snippet: .. RNA library preparation and sequencing Samples were quantified using Invitrogen Qubit Q32855 and RNA quality was assessed using the Agilent 2100 Bioanalyzer. .. The Illumina TruSeq RNA Sample Preparation Kit was used to generate libraries using ~2 μg of RNA.

    Article Title: Enhanced protection of C57 BL/6 vs Balb/c mice to melanoma liver metastasis is mediated by NK cells
    Article Snippet: The concentration and quality of the RNA was measured with the Qubit® RNA HS Assay Kit (Thermo Fisher Scientific) and the RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). .. Afterwards, 600 ng RNA were used to prepare the sequencing libraries with the TruSeq RNA Sample Preparation V2 kit (Illumina, San Diego, CA, USA) and subjected to sequencing 2 × 50 bp on a HiSeq2500 system (Illumina).

    Article Title: Gene expression in the mixotrophic prymnesiophyte, Prymnesium parvum, responds to prey availability
    Article Snippet: .. RNA was quantified again at the sequencing center using Invitrogen Qubit Q32855, and RNA quality was assessed using the Agilent 2100 Bioanalyzer. cDNA libraries were made from 2 μg RNA using Illumina's TruSeq RNA Sample Preparation Kit. ..

    Article Title: Tubulin C-terminal Post-translational Modifications Do Not Occur in Wood Forming Tissue of Populus
    Article Snippet: RNA-Seq Analysis The total RNA was extracted with the Direct-zol RNA Kit (Zymo Research) using Plant RNA Reagent (Life Technologies) and quantified with the Qubit RNA HS Assay Kit on a Qubit fluorometer (Life Technologies). .. The data are available at NCBI Sequence Read Archive under accession number SRP076604.

    DNA Extraction:

    Article Title: Comparative Genomics of Completely Sequenced Lactobacillus helveticus Genomes Provides Insights into Strain-Specific Genes and Resolves Metagenomics Data Down to the Strain Level
    Article Snippet: Paragraph title: Bacterial culture and genomic DNA extraction ... The gDNA purity was assessed by controlling for RNA contamination using the Qubit RNA HS Assay kit (Thermo Scientific, Massachusetts, USA).

    RNA Sequencing Assay:

    Article Title: Cultivation and Transcriptional Analysis of a Canonical Nitrospira Under Stable Growth Conditions
    Article Snippet: RNA quantity was controlled using the Qubit RNA HS Assay Kit (Thermo Fisher Scientific). .. Transcriptome libraries were prepared with the Ion Total RNA-Seq Kit v2 (Ion Torrent by Thermo Fisher Scientific).

    Article Title: Datasets for next-generation sequencing of DNA and RNA from urine and plasma of patients with prostate cancer
    Article Snippet: RNA concentration was determined by fluorometer Qubit 2.0 using Qubit RNA HS Assay Kit (Thermo Fisher). .. Transcriptomic libraries were constructed using Ion Total RNA-Seq Kit v2 (Life Technologies) with the following modifications of the protocol.

    Article Title: Tubulin C-terminal Post-translational Modifications Do Not Occur in Wood Forming Tissue of Populus
    Article Snippet: .. RNA-Seq Analysis The total RNA was extracted with the Direct-zol RNA Kit (Zymo Research) using Plant RNA Reagent (Life Technologies) and quantified with the Qubit RNA HS Assay Kit on a Qubit fluorometer (Life Technologies). .. RNA-Seq libraries were prepared using the Illumina TruSeq Stranded RNA LT Kit and sequenced on an Illumina NextSeq 500 at the Georgia Genomics Facility of University of Georgia.

    RNA HS Assay:

    Article Title: Comparative evaluation of rRNA depletion procedures for the improved analysis of bacterial biofilm and mixed pathogen culture transcriptomes
    Article Snippet: .. RNA and DNA quantity and quality assessment Where indicated, RNA or DNA was quantified using Qubit RNA HS Assay Kit and Qubit dsDNA HS Assay Kit (Life Technologies), respectively, on the Qubit 2.0 Fluorometer (Life Technologies). .. In addition, where indicated, fragment size distribution of RNA or DNA samples was assessed on the Agilent 2100 BioAnalyzer using the Agilent RNA 6000 Pico Kit or Agilent High Sensitivity DNA Kit (Agilent Technologies), respectively.

    Article Title: Robust detection of immune transcripts in FFPE samples using targeted RNA sequencing
    Article Snippet: .. RNA was quantified by means of the Qubit RNA HS Assay Kit (Thermo Fisher Scientific). .. RNAseq library preparation, quantification, pooling and sequencing Oncomine™ Immune Response Research Assay libraries were prepared using the Ion AmpliSeq™ targeted sequencing technology (Thermo Fisher Scientific), as per manufacturer's instructions.

    Article Title: Cultivation and Transcriptional Analysis of a Canonical Nitrospira Under Stable Growth Conditions
    Article Snippet: .. RNA quantity was controlled using the Qubit RNA HS Assay Kit (Thermo Fisher Scientific). .. Next, ribosomal RNA was depleted using the MICROBExpress kit (Thermo Fisher Scientific) following the manufacturer’s protocol.

    Article Title: Transcriptional Landscape of a blaKPC-2 Plasmid and Response to Imipenem Exposure in Escherichia coli TOP10
    Article Snippet: .. Ribodepleted RNA concentration was measured using Qubit™ RNA HS assay kit (Invitrogen). .. RNA library and sequencing cDNA libraries were prepared using the NEBNext® Ultra Directional RNA Library Prep Kit for Illumina protocol (New England Biolabs, MA, USA).

    Article Title: Comparative Genomics of Completely Sequenced Lactobacillus helveticus Genomes Provides Insights into Strain-Specific Genes and Resolves Metagenomics Data Down to the Strain Level
    Article Snippet: .. The gDNA purity was assessed by controlling for RNA contamination using the Qubit RNA HS Assay kit (Thermo Scientific, Massachusetts, USA). .. The gDNA was sequenced on the PacBio RS II platform (three SMRT cells per strain, P6-C4 chemistry, size-selection step (10 kb inserts) with BluePippin; for details, see Supplementary Table ).

    Article Title: Datasets for next-generation sequencing of DNA and RNA from urine and plasma of patients with prostate cancer
    Article Snippet: .. RNA concentration was determined by fluorometer Qubit 2.0 using Qubit RNA HS Assay Kit (Thermo Fisher). .. Transcriptomic libraries were constructed using Ion Total RNA-Seq Kit v2 (Life Technologies) with the following modifications of the protocol.

    Article Title: Enhanced protection of C57 BL/6 vs Balb/c mice to melanoma liver metastasis is mediated by NK cells
    Article Snippet: .. The concentration and quality of the RNA was measured with the Qubit® RNA HS Assay Kit (Thermo Fisher Scientific) and the RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). .. Afterwards, 600 ng RNA were used to prepare the sequencing libraries with the TruSeq RNA Sample Preparation V2 kit (Illumina, San Diego, CA, USA) and subjected to sequencing 2 × 50 bp on a HiSeq2500 system (Illumina).

    Article Title: Transcriptome analysis of the adult human Klinefelter testis and cellularity-matched controls reveals disturbed differentiation of Sertoli- and Leydig cells
    Article Snippet: .. To assess the quantity and quality of the samples, the Qubit RNA HS Assay Kit (Thermo Fisher Scientific) and the Agilent RNA 6000 Pico Kit (Agilent Technologies, CA, USA), respectively, were used. .. Library preparation The Ovation Human FFPE RNA-Seq Multiplex Systems (NuGEN, Leek, Netherlands) were used for library preparation from 150–200 ng of total RNA according to the manufacturer’s recommendations with the following modifications: The cDNA samples were fragmented to yield sizes of approximately 150 bp using the Covaris E210 sonicator (Covaris Ltd., Brighton, UK).

    Article Title: Cellular and Molecular Responses Unique to Major Injury Are Dispensable for Planarian Regeneration
    Article Snippet: .. Following RNA purification and resuspension in MilliQ H2 O, concentrations for each sample were determined using the Qubit RNA HS Assay Kit ( Life Technologies ). .. 1 μg RNA input was used to prepare cDNA with the SuperScript III Reverse Transcriptase kit ( Invitrogen ).

    Article Title: RNA-sequence data normalization through in silico prediction of reference genes: the bacterial response to DNA damage as case study
    Article Snippet: .. qRT-PCR RNA concentrations were determined with a Qubit 2.0 Fluorometer (Invitrogen) using the Qubit RNA HS Assay Kit (Molecular Probes). .. Primers for qRT-PCR were optimized using primer design software (Additional file ).

    Isolation:

    Article Title: Transcriptional Landscape of a blaKPC-2 Plasmid and Response to Imipenem Exposure in Escherichia coli TOP10
    Article Snippet: Paragraph title: RNA isolation and purification ... Ribodepleted RNA concentration was measured using Qubit™ RNA HS assay kit (Invitrogen).

    Article Title: Comparative Genomics of Completely Sequenced Lactobacillus helveticus Genomes Provides Insights into Strain-Specific Genes and Resolves Metagenomics Data Down to the Strain Level
    Article Snippet: Genomic DNA (gDNA) was isolated as described elsewhere (Moser et al., ), and the concentration determined (Qubit dsDNA BR Assay kit). .. The gDNA purity was assessed by controlling for RNA contamination using the Qubit RNA HS Assay kit (Thermo Scientific, Massachusetts, USA).

    Article Title: Enhanced protection of C57 BL/6 vs Balb/c mice to melanoma liver metastasis is mediated by NK cells
    Article Snippet: Next generation sequencing Total RNA was isolated from ∼ 50 mg fresh frozen tissue using the RNeasy Mini Kit (Qiagen, Hilden, Germany). .. The concentration and quality of the RNA was measured with the Qubit® RNA HS Assay Kit (Thermo Fisher Scientific) and the RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: Transcriptome analysis of the adult human Klinefelter testis and cellularity-matched controls reveals disturbed differentiation of Sertoli- and Leydig cells
    Article Snippet: RNA extraction Total RNA was extracted using the Recover All Total Nucleic Acid Isolation Kit for FFPE (Thermo Fisher Scientific, MS, USA) according to the manufacturer’s recommendations. .. To assess the quantity and quality of the samples, the Qubit RNA HS Assay Kit (Thermo Fisher Scientific) and the Agilent RNA 6000 Pico Kit (Agilent Technologies, CA, USA), respectively, were used.

    Article Title: Cellular and Molecular Responses Unique to Major Injury Are Dispensable for Planarian Regeneration
    Article Snippet: Total RNA was isolated in 1mL Trizol ( Life Technologies ) as per manufacturer’s instructions. .. Following RNA purification and resuspension in MilliQ H2 O, concentrations for each sample were determined using the Qubit RNA HS Assay Kit ( Life Technologies ).

    Purification:

    Article Title: Robust detection of immune transcripts in FFPE samples using targeted RNA sequencing
    Article Snippet: Standard de-crosslinking and column purification steps were performed to remove proteins and other cellular components prior to RNA elution in water. .. RNA was quantified by means of the Qubit RNA HS Assay Kit (Thermo Fisher Scientific).

    Article Title: Transcriptional Landscape of a blaKPC-2 Plasmid and Response to Imipenem Exposure in Escherichia coli TOP10
    Article Snippet: Paragraph title: RNA isolation and purification ... Ribodepleted RNA concentration was measured using Qubit™ RNA HS assay kit (Invitrogen).

    Article Title: Cellular and Molecular Responses Unique to Major Injury Are Dispensable for Planarian Regeneration
    Article Snippet: .. Following RNA purification and resuspension in MilliQ H2 O, concentrations for each sample were determined using the Qubit RNA HS Assay Kit ( Life Technologies ). .. 1 μg RNA input was used to prepare cDNA with the SuperScript III Reverse Transcriptase kit ( Invitrogen ).

    Polymerase Chain Reaction:

    Article Title: Transcriptional Landscape of a blaKPC-2 Plasmid and Response to Imipenem Exposure in Escherichia coli TOP10
    Article Snippet: Verification of complete removal of any contaminating DNA was performed via PCR amplification of recA housekeeping gene. .. Ribodepleted RNA concentration was measured using Qubit™ RNA HS assay kit (Invitrogen).

    Quantitative RT-PCR:

    Article Title: Cellular and Molecular Responses Unique to Major Injury Are Dispensable for Planarian Regeneration
    Article Snippet: Paragraph title: Quantitative real-time PCR (qRT-PCR) ... Following RNA purification and resuspension in MilliQ H2 O, concentrations for each sample were determined using the Qubit RNA HS Assay Kit ( Life Technologies ).

    Article Title: The Role of Circulating MicroRNA in the Regulation of Beta Cell Function and Insulin Resistance among Indians with Type 2 Diabetes
    Article Snippet: Quantitation of the small RNA was done using the Qubit Hs RNA assay reagents (Invitrogen #Q32852-Qubit Hs RNA assay kit) on a fluorimeter (Qubit® 2.0 Fluorometer Cat# Q32866). miRNA was converted to cDNA using stem loop primers specific for the chosen miRNA using Taqman MicroRNA Reverse Transcription Kit (Applied Biosystems, #4366596) according to published protocols. .. Quantitative Real Time PCR: Taqman MicroRNA inventoried assays for q-RT-PCR (Applied Biosystems, #4427975) were used for miR9, miR30d, miR1, miR29a, miR-133a, and miR143; these assay kits comprise stem loop primers for cDNA conversion as well as TaqMan primer-probes for qRT-PCR analysis.

    Article Title: RNA-sequence data normalization through in silico prediction of reference genes: the bacterial response to DNA damage as case study
    Article Snippet: .. qRT-PCR RNA concentrations were determined with a Qubit 2.0 Fluorometer (Invitrogen) using the Qubit RNA HS Assay Kit (Molecular Probes). .. Primers for qRT-PCR were optimized using primer design software (Additional file ).

    Agarose Gel Electrophoresis:

    Article Title: Cultivation and Transcriptional Analysis of a Canonical Nitrospira Under Stable Growth Conditions
    Article Snippet: RNA purity was confirmed on an ethidium bromide stained 0.9% agarose gel. .. RNA quantity was controlled using the Qubit RNA HS Assay Kit (Thermo Fisher Scientific).

    Mouse Assay:

    Article Title: Enhanced protection of C57 BL/6 vs Balb/c mice to melanoma liver metastasis is mediated by NK cells
    Article Snippet: The concentration and quality of the RNA was measured with the Qubit® RNA HS Assay Kit (Thermo Fisher Scientific) and the RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). .. Differential gene expression between metastasized livers of Balb/c and C57 BL/6 mice was calculated using the R bioconductor package DESeq2 using the raw read counts of each gene as input.

    Software:

    Article Title: RNA-sequence data normalization through in silico prediction of reference genes: the bacterial response to DNA damage as case study
    Article Snippet: qRT-PCR RNA concentrations were determined with a Qubit 2.0 Fluorometer (Invitrogen) using the Qubit RNA HS Assay Kit (Molecular Probes). .. Primers for qRT-PCR were optimized using primer design software (Additional file ).

    SYBR Green Assay:

    Article Title: RNA-sequence data normalization through in silico prediction of reference genes: the bacterial response to DNA damage as case study
    Article Snippet: qRT-PCR RNA concentrations were determined with a Qubit 2.0 Fluorometer (Invitrogen) using the Qubit RNA HS Assay Kit (Molecular Probes). .. The Brilliant III Ultra-Fast SYBR Green QRT-PCR Master Mix (Agilent Technologies) was applied to perform an ultra-fast one-step protocol on a StepOnePlus Real-Time PCR System (Applied Biosystems/Life Technologies) using the following settings for amplification: 50 °C - 10 min, 95 °C - 3 min, 45× (95 °C - 5 s, 60 °C - 10 s).

    RNA Extraction:

    Article Title: Robust detection of immune transcripts in FFPE samples using targeted RNA sequencing
    Article Snippet: Paragraph title: RNA extraction ... RNA was quantified by means of the Qubit RNA HS Assay Kit (Thermo Fisher Scientific).

    Article Title: Cultivation and Transcriptional Analysis of a Canonical Nitrospira Under Stable Growth Conditions
    Article Snippet: Paragraph title: RNA Extraction and Transcriptome Sequencing ... RNA quantity was controlled using the Qubit RNA HS Assay Kit (Thermo Fisher Scientific).

    Article Title: Gene expression in the mixotrophic prymnesiophyte, Prymnesium parvum, responds to prey availability
    Article Snippet: Paragraph title: RNA extraction and cDNA production ... RNA was quantified again at the sequencing center using Invitrogen Qubit Q32855, and RNA quality was assessed using the Agilent 2100 Bioanalyzer. cDNA libraries were made from 2 μg RNA using Illumina's TruSeq RNA Sample Preparation Kit.

    Article Title: Transcriptome analysis of the adult human Klinefelter testis and cellularity-matched controls reveals disturbed differentiation of Sertoli- and Leydig cells
    Article Snippet: Paragraph title: RNA extraction ... To assess the quantity and quality of the samples, the Qubit RNA HS Assay Kit (Thermo Fisher Scientific) and the Agilent RNA 6000 Pico Kit (Agilent Technologies, CA, USA), respectively, were used.

    Sample Prep:

    Article Title: Comparative Transcriptome Analysis of Four Prymnesiophyte Algae
    Article Snippet: Library Preparation and Sequencing All samples were quantified again at the sequencing center using Invitrogen Qubit Q32855 and RNA quality was assessed using the Agilent 2100 Bioanalyzer. .. Libraries were made from 2 µg RNA using Illumina's TruSeq RNA Sample Preparation Kit.

    Article Title: De novo assembly of Aureococcus anophagefferens transcriptomes reveals diverse responses to the low nutrient and low light conditions present during blooms
    Article Snippet: RNA library preparation and sequencing Samples were quantified using Invitrogen Qubit Q32855 and RNA quality was assessed using the Agilent 2100 Bioanalyzer. .. The Illumina TruSeq RNA Sample Preparation Kit was used to generate libraries using ~2 μg of RNA.

    Article Title: Enhanced protection of C57 BL/6 vs Balb/c mice to melanoma liver metastasis is mediated by NK cells
    Article Snippet: The concentration and quality of the RNA was measured with the Qubit® RNA HS Assay Kit (Thermo Fisher Scientific) and the RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). .. Afterwards, 600 ng RNA were used to prepare the sequencing libraries with the TruSeq RNA Sample Preparation V2 kit (Illumina, San Diego, CA, USA) and subjected to sequencing 2 × 50 bp on a HiSeq2500 system (Illumina).

    Article Title: Gene expression in the mixotrophic prymnesiophyte, Prymnesium parvum, responds to prey availability
    Article Snippet: .. RNA was quantified again at the sequencing center using Invitrogen Qubit Q32855, and RNA quality was assessed using the Agilent 2100 Bioanalyzer. cDNA libraries were made from 2 μg RNA using Illumina's TruSeq RNA Sample Preparation Kit. ..

    Next-Generation Sequencing:

    Article Title: Enhanced protection of C57 BL/6 vs Balb/c mice to melanoma liver metastasis is mediated by NK cells
    Article Snippet: Paragraph title: Next generation sequencing ... The concentration and quality of the RNA was measured with the Qubit® RNA HS Assay Kit (Thermo Fisher Scientific) and the RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA).

    Quantitation Assay:

    Article Title: The Role of Circulating MicroRNA in the Regulation of Beta Cell Function and Insulin Resistance among Indians with Type 2 Diabetes
    Article Snippet: .. Quantitation of the small RNA was done using the Qubit Hs RNA assay reagents (Invitrogen #Q32852-Qubit Hs RNA assay kit) on a fluorimeter (Qubit® 2.0 Fluorometer Cat# Q32866). miRNA was converted to cDNA using stem loop primers specific for the chosen miRNA using Taqman MicroRNA Reverse Transcription Kit (Applied Biosystems, #4366596) according to published protocols. .. Concentration of 50–100 ng of RNA was used for the conversion of miRNA to cDNA according to manufacturer's instructions using Veriti 96 well thermal cycler (Applied Biosystems).

    Sampling:

    Article Title: Cultivation and Transcriptional Analysis of a Canonical Nitrospira Under Stable Growth Conditions
    Article Snippet: RNA Extraction and Transcriptome Sequencing Transcriptomic analyses were performed using three technical replicates for each of the three sampling time points. .. RNA quantity was controlled using the Qubit RNA HS Assay Kit (Thermo Fisher Scientific).

    Concentration Assay:

    Article Title: Transcriptional Landscape of a blaKPC-2 Plasmid and Response to Imipenem Exposure in Escherichia coli TOP10
    Article Snippet: .. Ribodepleted RNA concentration was measured using Qubit™ RNA HS assay kit (Invitrogen). .. RNA library and sequencing cDNA libraries were prepared using the NEBNext® Ultra Directional RNA Library Prep Kit for Illumina protocol (New England Biolabs, MA, USA).

    Article Title: Comparative Genomics of Completely Sequenced Lactobacillus helveticus Genomes Provides Insights into Strain-Specific Genes and Resolves Metagenomics Data Down to the Strain Level
    Article Snippet: Genomic DNA (gDNA) was isolated as described elsewhere (Moser et al., ), and the concentration determined (Qubit dsDNA BR Assay kit). .. The gDNA purity was assessed by controlling for RNA contamination using the Qubit RNA HS Assay kit (Thermo Scientific, Massachusetts, USA).

    Article Title: Datasets for next-generation sequencing of DNA and RNA from urine and plasma of patients with prostate cancer
    Article Snippet: .. RNA concentration was determined by fluorometer Qubit 2.0 using Qubit RNA HS Assay Kit (Thermo Fisher). .. Transcriptomic libraries were constructed using Ion Total RNA-Seq Kit v2 (Life Technologies) with the following modifications of the protocol.

    Article Title: Enhanced protection of C57 BL/6 vs Balb/c mice to melanoma liver metastasis is mediated by NK cells
    Article Snippet: .. The concentration and quality of the RNA was measured with the Qubit® RNA HS Assay Kit (Thermo Fisher Scientific) and the RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). .. Afterwards, 600 ng RNA were used to prepare the sequencing libraries with the TruSeq RNA Sample Preparation V2 kit (Illumina, San Diego, CA, USA) and subjected to sequencing 2 × 50 bp on a HiSeq2500 system (Illumina).

    Article Title: The Role of Circulating MicroRNA in the Regulation of Beta Cell Function and Insulin Resistance among Indians with Type 2 Diabetes
    Article Snippet: Quantitation of the small RNA was done using the Qubit Hs RNA assay reagents (Invitrogen #Q32852-Qubit Hs RNA assay kit) on a fluorimeter (Qubit® 2.0 Fluorometer Cat# Q32866). miRNA was converted to cDNA using stem loop primers specific for the chosen miRNA using Taqman MicroRNA Reverse Transcription Kit (Applied Biosystems, #4366596) according to published protocols. .. Concentration of 50–100 ng of RNA was used for the conversion of miRNA to cDNA according to manufacturer's instructions using Veriti 96 well thermal cycler (Applied Biosystems).

    Staining:

    Article Title: Cultivation and Transcriptional Analysis of a Canonical Nitrospira Under Stable Growth Conditions
    Article Snippet: RNA purity was confirmed on an ethidium bromide stained 0.9% agarose gel. .. RNA quantity was controlled using the Qubit RNA HS Assay Kit (Thermo Fisher Scientific).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Thermo Fisher qubit protein assay kit
    Qubit Protein Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 413 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit protein assay kit/product/Thermo Fisher
    Average 90 stars, based on 413 article reviews
    Price from $9.99 to $1999.99
    qubit protein assay kit - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher qubit dsdna hs assay kit
    Qubit Dsdna Hs Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 793 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit dsdna hs assay kit/product/Thermo Fisher
    Average 90 stars, based on 793 article reviews
    Price from $9.99 to $1999.99
    qubit dsdna hs assay kit - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    Image Search Results