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Agilent technologies qubit 2 0 fluorometer
Qubit 2 0 Fluorometer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 10 article reviews
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qubit 2 0 fluorometer - by Bioz Stars, 2019-10
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Centrifugation:

Article Title: Landscape of the genome and host cell response of Mycobacterium shigaense reveals pathogenic features
Article Snippet: Each of the three mycobacterial species were cultured in Middlebrook 7H9 medium with Middlebrook OADC enrichment at 37 °C and then pelleted by centrifugation. .. RNA quantity and quality were estimated with a Qubit 2.0 Fluorometer and Agilent Technology 2100 Bioanalyzer, respectively.

Amplification:

Article Title: Gut Microbiota-Regulated Pharmacokinetics of Berberine and Active Metabolites in Beagle Dogs After Oral Administration
Article Snippet: The 16S rRNA genes were amplified using the specific primer of 16S V3-V4: 340F-805R to target the V3-V4 regions of 16S rRNA. .. The library quality was assessed on a Qubit 2.0 Fluorometer and Agilent Bioanalyzer 2100 system.

Article Title: Responses of intestinal virome to silver nanoparticles: safety assessment by classical virology, whole-genome sequencing and bioinformatics approaches
Article Snippet: After quantification of the products, equal concentrations of the amplified DNA from each experimental group were sequenced using next-generation sequencing platforms. .. The size selected final libraries were quantified with Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer.

Article Title: Comprehensive Transcriptome Analysis Reveals Competing Endogenous RNA Networks During Avian Leukosis Virus, Subgroup J-Induced Tumorigenesis in Chickens
Article Snippet: After selecting fragments of the desired length and amplification by PCR, the amplified cDNA libraries were qualified using an Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA, United States). .. Subsequently, reverse transcription and PCR were conducted to construct the cDNA library (150 bp), and then the libraries underwent quantification and quality assessment using a Qubit® 2.0 fluorometer and an Agilent 2100 Bioanalyzer (Agilent).

Article Title: Full-Length Transcriptome Survey and Expression Analysis of Cassia obtusifolia to Discover Putative Genes Related to Aurantio-Obtusin Biosynthesis, Seed Formation and Development, and Stress Response
Article Snippet: After PCR amplification, quality control, and purification, we performed size selection using BluePippin Size Selection System protocol and herein produced three fractions containing fragments of 1–2, 2–3, and 3–6 kb in length, respectively. .. The concentration and quality of the cDNA library were measured using Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer, respectively.

Article Title: Evaluating hybridization capture with RAD probes as a tool for museum genomics with historical bird specimens. Evaluating hybridization capture with RAD probes as a tool for museum genomics with historical bird specimens
Article Snippet: We used a 1–1.5× ratio of AmPure XP beads to remove small DNA fragments throughout the protocol and assessed DNA quantity and quality with a Qubit 2.0 fluorometer and an Agilent 2100 Bioanalyzer between all major steps. .. We used a 1–1.5× ratio of AmPure XP beads to remove small DNA fragments throughout the protocol and assessed DNA quantity and quality with a Qubit 2.0 fluorometer and an Agilent 2100 Bioanalyzer between all major steps.

Article Title: Transcriptome analysis of genes involved in defense against alkaline stress in roots of wild jujube (Ziziphus acidojujuba)
Article Snippet: After performing end repair and poly-adenylation, the mRNAs were ligated to adapters before PCR amplification. .. The purity and size of the libraries were checked by Qubit 2.0 Fluorometer and Agilent 2100 Bioanalyzer (Agilent Technologies).

Article Title: De novo Transcriptome Analysis and Molecular Marker Development of Two Hemarthria Species
Article Snippet: Next, PCR amplification was then performed using the Phusion High-Fidelity DNA polymerase in order to enrich the purified cDNA template. .. To ensure that the quality of the library was sufficient for sequencing, the concentration and insert size of the library was detected using a Qubit® 2.0 Fluorometer and Agilent 2100 Bioanalyzer, respectively.

Synthesized:

Article Title: Transcriptome analysis of genes involved in defense against alkaline stress in roots of wild jujube (Ziziphus acidojujuba)
Article Snippet: Then, second-strand was synthesized using Invitrogen DNA polymerase 1 (Invitrogen, Carlsbad, CA, USA). .. The purity and size of the libraries were checked by Qubit 2.0 Fluorometer and Agilent 2100 Bioanalyzer (Agilent Technologies).

Article Title: De novo Transcriptome Analysis and Molecular Marker Development of Two Hemarthria Species
Article Snippet: Next, second-strand cDNA was synthesized in a buffer containing dNTPs, DNA polymerase I, and RNaseH. .. To ensure that the quality of the library was sufficient for sequencing, the concentration and insert size of the library was detected using a Qubit® 2.0 Fluorometer and Agilent 2100 Bioanalyzer, respectively.

Cytometry:

Article Title: Endogenous Reprogramming of Alpha Cells into Beta Cells Induced by Viral Gene Therapy Reverses Autoimmune Diabetes
Article Snippet: Total RNA was extracted from alpha-cell-derived INS+ cells (Neo INS+) that were isolated 1 month after AAV-PM infusion in ALX-treated GCG-Cre; R26RTomato ; MIP-GFP mice, based on expression of tomato red (alpha-cell lineage) and GFP by flow cytometry. .. Each sample was assessed using Qubit 2.0 fluorometer and Agilent TapeStation 2200 for RNA quantity and quality.

Construct:

Article Title: Comprehensive Transcriptome Analysis Reveals Competing Endogenous RNA Networks During Avian Leukosis Virus, Subgroup J-Induced Tumorigenesis in Chickens
Article Snippet: For small RNA-Seq, small RNA fractions were ligated to 5′ and 3′ RNA adaptors. .. Subsequently, reverse transcription and PCR were conducted to construct the cDNA library (150 bp), and then the libraries underwent quantification and quality assessment using a Qubit® 2.0 fluorometer and an Agilent 2100 Bioanalyzer (Agilent). .. Finally, the libraries were sequenced on an Illumina HiSeqTM 2500 instrument (Illumina).

Article Title: RNA-seq-based analysis of drug-resistant Salmonella enterica serovar Typhimurium selected in vivo and in vitro
Article Snippet: The rRNA-depleted and RNA-fragmented libraries were constructed with sequential first- and second-strand cDNA synthesis, followed by end repair, adenylation of the 3' ends, ligation of adapters, and enrichment of the cDNA templates, according to experimental instructions. .. The quality and quantity of each libraries were determined with a Qubit® 2.0 Fluorometer and Agilent Bioanalyzer 2100, respectively.

Real-time Polymerase Chain Reaction:

Article Title: Sequencing and De Novo Assembly of the Asian Clam (Corbicula fluminea) Transcriptome Using the Illumina GAIIx Method
Article Snippet: The total RNA concentration and quality were determined and quantified using a QUBIT 2.0 fluorometer and an Agilent 2100 BioAnalyzer. .. For mRNA library construction and deep sequencing, RNA samples were prepared using the TruSeq RNA Sample Preparation Kit according to the manufacturer’s protocol.

Article Title: Novel genes associated with enhanced motility of Escherichia coli ST131
Article Snippet: Each library was purified using Agencourt® Ampure® XP magnetic beads. .. Verification and quantification of resulting libraries were calculated using a Qubit® 2.0 Fluorometer, 2100 Bioanalyser (Agilent Technologies) and qPCR (KAPA Biosciences). .. All libraries were pooled in equimolar to a final concentration of 3.2nM and submitted for sequencing on the MiSeq platform at the Queensland Centre for Medical Genomics (Institute for Molecular Bioscience, The University of Queensland).

Random Hexamer Labeling:

Article Title: Transcriptome analysis of genes involved in defense against alkaline stress in roots of wild jujube (Ziziphus acidojujuba)
Article Snippet: These cleaved mRNA fragments were used as templates for first-strand cDNA synthesis using the random hexamer primers and Superscript II reverse transcriptase (Invitrogen, Carlsbad, CA, USA). .. The purity and size of the libraries were checked by Qubit 2.0 Fluorometer and Agilent 2100 Bioanalyzer (Agilent Technologies).

Infection:

Article Title: Landscape of the genome and host cell response of Mycobacterium shigaense reveals pathogenic features
Article Snippet: For obtaining RNA from infected macrophages, triplicate wells of THP-1 cells were infected with mycobacteria and harvested at three time points, including 6, 24, and 48 h, as described in the previous section. .. RNA quantity and quality were estimated with a Qubit 2.0 Fluorometer and Agilent Technology 2100 Bioanalyzer, respectively.

Expressing:

Article Title: Endogenous Reprogramming of Alpha Cells into Beta Cells Induced by Viral Gene Therapy Reverses Autoimmune Diabetes
Article Snippet: Total RNA was extracted from alpha-cell-derived INS+ cells (Neo INS+) that were isolated 1 month after AAV-PM infusion in ALX-treated GCG-Cre; R26RTomato ; MIP-GFP mice, based on expression of tomato red (alpha-cell lineage) and GFP by flow cytometry. .. Each sample was assessed using Qubit 2.0 fluorometer and Agilent TapeStation 2200 for RNA quantity and quality.

Article Title: Murine models of Pneumocystis infection recapitulate human primary immune disorders
Article Snippet: RNA quantity and quality was assessed using Qubit 2.0 fluorometer and Agilent Bioanalyzer TapeStation 2200. .. Cluster generation and 75-bp paired-read single-indexed sequencing was performed on Illumina NextSeq 500’s.

Modification:

Article Title: Genome-Wide Discovery of Genes Required for Capsule Production by Uropathogenic Escherichia coli
Article Snippet: Genomic DNA from each sample (tests and controls) was subjected to library preparation by using the Nextera DNA library prep kit (Illumina) with slight modification to amplify and sequence Tn5 insertion sites ( ). .. Library verification and quantification were undertaken using a Qubit 2.0 fluorometer and 4200 Tapestation system (Agilent Technologies).

Hybridization:

Article Title: Evaluating hybridization capture with RAD probes as a tool for museum genomics with historical bird specimens. Evaluating hybridization capture with RAD probes as a tool for museum genomics with historical bird specimens
Article Snippet: Paragraph title: Library preparation, hybridization capture experiments, and sequencing ... We used a 1–1.5× ratio of AmPure XP beads to remove small DNA fragments throughout the protocol and assessed DNA quantity and quality with a Qubit 2.0 fluorometer and an Agilent 2100 Bioanalyzer between all major steps.

Flow Cytometry:

Article Title: Endogenous Reprogramming of Alpha Cells into Beta Cells Induced by Viral Gene Therapy Reverses Autoimmune Diabetes
Article Snippet: Total RNA was extracted from alpha-cell-derived INS+ cells (Neo INS+) that were isolated 1 month after AAV-PM infusion in ALX-treated GCG-Cre; R26RTomato ; MIP-GFP mice, based on expression of tomato red (alpha-cell lineage) and GFP by flow cytometry. .. Each sample was assessed using Qubit 2.0 fluorometer and Agilent TapeStation 2200 for RNA quantity and quality.

Article Title: Transcriptome analysis of genes involved in defense against alkaline stress in roots of wild jujube (Ziziphus acidojujuba)
Article Snippet: The purity and size of the libraries were checked by Qubit 2.0 Fluorometer and Agilent 2100 Bioanalyzer (Agilent Technologies). .. Finally, the adapter-ligated cDNA fragment libraries were run on an Illumina GAIIx analyzer to complete the cluster generation and primer hybridization.

Ligation:

Article Title: Comprehensive Transcriptome Analysis Reveals Competing Endogenous RNA Networks During Avian Leukosis Virus, Subgroup J-Induced Tumorigenesis in Chickens
Article Snippet: Thereafter, purification, end repair, and Illumina adaptor ligation of the cDNA were performed. .. Subsequently, reverse transcription and PCR were conducted to construct the cDNA library (150 bp), and then the libraries underwent quantification and quality assessment using a Qubit® 2.0 fluorometer and an Agilent 2100 Bioanalyzer (Agilent).

Article Title: RNA-seq-based analysis of drug-resistant Salmonella enterica serovar Typhimurium selected in vivo and in vitro
Article Snippet: The rRNA-depleted and RNA-fragmented libraries were constructed with sequential first- and second-strand cDNA synthesis, followed by end repair, adenylation of the 3' ends, ligation of adapters, and enrichment of the cDNA templates, according to experimental instructions. .. The quality and quantity of each libraries were determined with a Qubit® 2.0 Fluorometer and Agilent Bioanalyzer 2100, respectively.

Cell Culture:

Article Title: Landscape of the genome and host cell response of Mycobacterium shigaense reveals pathogenic features
Article Snippet: Each of the three mycobacterial species were cultured in Middlebrook 7H9 medium with Middlebrook OADC enrichment at 37 °C and then pelleted by centrifugation. .. RNA quantity and quality were estimated with a Qubit 2.0 Fluorometer and Agilent Technology 2100 Bioanalyzer, respectively.

Generated:

Article Title: Peptide vaccine immunotherapy biomarkers and response patterns in pediatric gliomas
Article Snippet: Each sample was assessed using a Qubit 2.0 fluorometer and Agilent 4 for RNA quantity and quality. .. Each sample was assessed using a Qubit 2.0 fluorometer and Agilent 4 for RNA quantity and quality.

Article Title: Gut Microbiota-Regulated Pharmacokinetics of Berberine and Active Metabolites in Beagle Dogs After Oral Administration
Article Snippet: Sequencing libraries were generated by using a NEXTflex Rapid Illumina DNA-seq Kit from New England Biolabs following manufacturer's recommendations and adding index codes. .. The library quality was assessed on a Qubit 2.0 Fluorometer and Agilent Bioanalyzer 2100 system.

Article Title: Responses of intestinal virome to silver nanoparticles: safety assessment by classical virology, whole-genome sequencing and bioinformatics approaches
Article Snippet: The size selected final libraries were quantified with Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer. .. The size selected final libraries were quantified with Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer.

Polymerase Chain Reaction:

Article Title: Endogenous Reprogramming of Alpha Cells into Beta Cells Induced by Viral Gene Therapy Reverses Autoimmune Diabetes
Article Snippet: Each sample was assessed using Qubit 2.0 fluorometer and Agilent TapeStation 2200 for RNA quantity and quality. .. Each sample was assessed using Qubit 2.0 fluorometer and Agilent TapeStation 2200 for RNA quantity and quality.

Article Title: Gut Microbiota-Regulated Pharmacokinetics of Berberine and Active Metabolites in Beagle Dogs After Oral Administration
Article Snippet: Then, the mixture of PCR products was purified with a GeneJET Gel Extraction Kit. .. The library quality was assessed on a Qubit 2.0 Fluorometer and Agilent Bioanalyzer 2100 system.

Article Title: Transcriptome analyses provide insights into the difference of alkaloids biosynthesis in the Chinese goldthread (Coptis chinensis Franch.) from different biotopes
Article Snippet: PCR amplifications of fragments were performed to create final cDNA libraries for sequencing. .. After library construction, the concentration and insert size of the three libraries were tested using Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer, respectively.

Article Title: Transcriptomic profiles of human foreskin fibroblast cells in response to orf virus
Article Snippet: The quantification and qualification of the library were carried out using Qubit® 2.0 Fluorometer and Agilent Bioanalyzer 2100 (con.=3.5-5.8ng/μL, peak length.=347-367 bp). .. The quantification and qualification of the library were carried out using Qubit® 2.0 Fluorometer and Agilent Bioanalyzer 2100 (con.=3.5-5.8ng/μL, peak length.=347-367 bp).

Article Title: Responses of intestinal virome to silver nanoparticles: safety assessment by classical virology, whole-genome sequencing and bioinformatics approaches
Article Snippet: Fragmented DNA was used for preparing uniquely indexed sample library using the TruSeq DNA PCR-Free Sample Preparation kit (Illumina), as recommended by the manufacturer and the final libraries were quantified with Qubit and 2100 Bioanalyzer. .. The size selected final libraries were quantified with Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer.

Article Title: Genome-Wide Discovery of Genes Required for Capsule Production by Uropathogenic Escherichia coli
Article Snippet: The PCR enrichment step, for which we used a custom transposon-specific primer to enrich for transposon insertion sites and an index primer (one index per sample) to allow for multiplexing sequencing, was performed at 72°C for 3 min and 98°C for 30 s, followed by 22 cycles of 98°C for 10 s, 63°C for 30 s, and 72°C for 1 min. Each library was purified using Agencourt Ampure XP magnetic beads. .. Library verification and quantification were undertaken using a Qubit 2.0 fluorometer and 4200 Tapestation system (Agilent Technologies).

Article Title: Comprehensive Transcriptome Analysis Reveals Competing Endogenous RNA Networks During Avian Leukosis Virus, Subgroup J-Induced Tumorigenesis in Chickens
Article Snippet: For small RNA-Seq, small RNA fractions were ligated to 5′ and 3′ RNA adaptors. .. Subsequently, reverse transcription and PCR were conducted to construct the cDNA library (150 bp), and then the libraries underwent quantification and quality assessment using a Qubit® 2.0 fluorometer and an Agilent 2100 Bioanalyzer (Agilent). .. Finally, the libraries were sequenced on an Illumina HiSeqTM 2500 instrument (Illumina).

Article Title: Full-Length Transcriptome Survey and Expression Analysis of Cassia obtusifolia to Discover Putative Genes Related to Aurantio-Obtusin Biosynthesis, Seed Formation and Development, and Stress Response
Article Snippet: After PCR amplification, quality control, and purification, we performed size selection using BluePippin Size Selection System protocol and herein produced three fractions containing fragments of 1–2, 2–3, and 3–6 kb in length, respectively. .. The concentration and quality of the cDNA library were measured using Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer, respectively.

Article Title: Transcriptome analysis of genes involved in defense against alkaline stress in roots of wild jujube (Ziziphus acidojujuba)
Article Snippet: After performing end repair and poly-adenylation, the mRNAs were ligated to adapters before PCR amplification. .. The purity and size of the libraries were checked by Qubit 2.0 Fluorometer and Agilent 2100 Bioanalyzer (Agilent Technologies).

Article Title: De novo Transcriptome Analysis and Molecular Marker Development of Two Hemarthria Species
Article Snippet: Next, PCR amplification was then performed using the Phusion High-Fidelity DNA polymerase in order to enrich the purified cDNA template. .. To ensure that the quality of the library was sufficient for sequencing, the concentration and insert size of the library was detected using a Qubit® 2.0 Fluorometer and Agilent 2100 Bioanalyzer, respectively.

Article Title: Novel genes associated with enhanced motility of Escherichia coli ST131
Article Snippet: The PCR enrichment step was run using index primer 1 (one index per sample) and a custom transposon specific primer 4844 ( 5’-AATGATACGGCGACCACCGAGATCTACACTAGATCGCaacttcggaataggaactaagg-3’ ) to enrich for transposon insertion sites and allow for multiplexing sequencing; the thermocycler program is 72°C for 3 minutes, 98°C for 30 seconds followed by 22 cycles of 98°C for 10 seconds, 63°C for 30 seconds and 72°C for 1 minute. .. Verification and quantification of resulting libraries were calculated using a Qubit® 2.0 Fluorometer, 2100 Bioanalyser (Agilent Technologies) and qPCR (KAPA Biosciences).

DNA Sequencing:

Article Title: Gut Microbiota-Regulated Pharmacokinetics of Berberine and Active Metabolites in Beagle Dogs After Oral Administration
Article Snippet: Sequencing libraries were generated by using a NEXTflex Rapid Illumina DNA-seq Kit from New England Biolabs following manufacturer's recommendations and adding index codes. .. The library quality was assessed on a Qubit 2.0 Fluorometer and Agilent Bioanalyzer 2100 system.

Sequencing:

Article Title: Endogenous Reprogramming of Alpha Cells into Beta Cells Induced by Viral Gene Therapy Reverses Autoimmune Diabetes
Article Snippet: Each sample was assessed using Qubit 2.0 fluorometer and Agilent TapeStation 2200 for RNA quantity and quality. .. These cDNA fragments are then purified and enriched with PCR to create the final cDNA library.

Article Title: Gut Microbiota-Regulated Pharmacokinetics of Berberine and Active Metabolites in Beagle Dogs After Oral Administration
Article Snippet: Sequencing libraries were generated by using a NEXTflex Rapid Illumina DNA-seq Kit from New England Biolabs following manufacturer's recommendations and adding index codes. .. The library quality was assessed on a Qubit 2.0 Fluorometer and Agilent Bioanalyzer 2100 system.

Article Title: Transcriptome analyses provide insights into the difference of alkaloids biosynthesis in the Chinese goldthread (Coptis chinensis Franch.) from different biotopes
Article Snippet: Paragraph title: RNA isolation, cDNA library preparation and Illumina sequencing ... After library construction, the concentration and insert size of the three libraries were tested using Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer, respectively.

Article Title: Murine models of Pneumocystis infection recapitulate human primary immune disorders
Article Snippet: RNA quantity and quality was assessed using Qubit 2.0 fluorometer and Agilent Bioanalyzer TapeStation 2200. .. The cDNA libraries were pooled at a final concentration 1.8 pM.

Article Title: Landscape of the genome and host cell response of Mycobacterium shigaense reveals pathogenic features
Article Snippet: Paragraph title: RNA isolation, QC and sequencing ... RNA quantity and quality were estimated with a Qubit 2.0 Fluorometer and Agilent Technology 2100 Bioanalyzer, respectively.

Article Title: Transcriptomic profiles of human foreskin fibroblast cells in response to orf virus
Article Snippet: The quantification and qualification of the library were carried out using Qubit® 2.0 Fluorometer and Agilent Bioanalyzer 2100 (con.=3.5-5.8ng/μL, peak length.=347-367 bp). .. The cDNA libraries were pooled at a concentration of 10 pM prior to clustering.

Article Title: Responses of intestinal virome to silver nanoparticles: safety assessment by classical virology, whole-genome sequencing and bioinformatics approaches
Article Snippet: Prior to sequencing, the extracted genomic DNA was fragmented with M220 Ultrasonicator. .. The size selected final libraries were quantified with Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer.

Article Title: Genome-Wide Discovery of Genes Required for Capsule Production by Uropathogenic Escherichia coli
Article Snippet: The PCR enrichment step, for which we used a custom transposon-specific primer to enrich for transposon insertion sites and an index primer (one index per sample) to allow for multiplexing sequencing, was performed at 72°C for 3 min and 98°C for 30 s, followed by 22 cycles of 98°C for 10 s, 63°C for 30 s, and 72°C for 1 min. Each library was purified using Agencourt Ampure XP magnetic beads. .. Library verification and quantification were undertaken using a Qubit 2.0 fluorometer and 4200 Tapestation system (Agilent Technologies).

Article Title: Sequencing and De Novo Assembly of the Asian Clam (Corbicula fluminea) Transcriptome Using the Illumina GAIIx Method
Article Snippet: Paragraph title: RNA isolation and Illumina sequencing ... The total RNA concentration and quality were determined and quantified using a QUBIT 2.0 fluorometer and an Agilent 2100 BioAnalyzer.

Article Title: Comprehensive Transcriptome Analysis Reveals Competing Endogenous RNA Networks During Avian Leukosis Virus, Subgroup J-Induced Tumorigenesis in Chickens
Article Snippet: Paragraph title: High-Throughput Sequencing ... Subsequently, reverse transcription and PCR were conducted to construct the cDNA library (150 bp), and then the libraries underwent quantification and quality assessment using a Qubit® 2.0 fluorometer and an Agilent 2100 Bioanalyzer (Agilent).

Article Title: RNA-seq-based analysis of drug-resistant Salmonella enterica serovar Typhimurium selected in vivo and in vitro
Article Snippet: Paragraph title: Library preparation and sequencing ... The quality and quantity of each libraries were determined with a Qubit® 2.0 Fluorometer and Agilent Bioanalyzer 2100, respectively.

Article Title: Full-Length Transcriptome Survey and Expression Analysis of Cassia obtusifolia to Discover Putative Genes Related to Aurantio-Obtusin Biosynthesis, Seed Formation and Development, and Stress Response
Article Snippet: Paragraph title: 4.4. Single-Molecule Real-Time Library Construction and Sequencing ... The concentration and quality of the cDNA library were measured using Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer, respectively.

Article Title: Evaluating hybridization capture with RAD probes as a tool for museum genomics with historical bird specimens. Evaluating hybridization capture with RAD probes as a tool for museum genomics with historical bird specimens
Article Snippet: Paragraph title: Library preparation, hybridization capture experiments, and sequencing ... We used a 1–1.5× ratio of AmPure XP beads to remove small DNA fragments throughout the protocol and assessed DNA quantity and quality with a Qubit 2.0 fluorometer and an Agilent 2100 Bioanalyzer between all major steps.

Article Title: Transcriptome analysis of genes involved in defense against alkaline stress in roots of wild jujube (Ziziphus acidojujuba)
Article Snippet: Paragraph title: Illumina sequencing ... The purity and size of the libraries were checked by Qubit 2.0 Fluorometer and Agilent 2100 Bioanalyzer (Agilent Technologies).

Article Title: De novo Transcriptome Analysis and Molecular Marker Development of Two Hemarthria Species
Article Snippet: Next, PCR amplification was then performed using the Phusion High-Fidelity DNA polymerase in order to enrich the purified cDNA template. .. To ensure that the quality of the library was sufficient for sequencing, the concentration and insert size of the library was detected using a Qubit® 2.0 Fluorometer and Agilent 2100 Bioanalyzer, respectively. .. Additionally, the effective concentration of the library was accurately quantified using quantitative PCR (Q-PCR).

Article Title: Prognostic and biological significance of the proangiogenic factor EGFL7 in acute myeloid leukemia
Article Snippet: In brief, extracted total RNA was assessed for quality on an Agilent 2100 Bioanalyzer (BioA) using the RNA 6000 Nano chip and for quantity on a Qubit 2.0 Fluorometer (Agilent Technologies) using the RNA HS Assay Kit. .. In brief, extracted total RNA was assessed for quality on an Agilent 2100 Bioanalyzer (BioA) using the RNA 6000 Nano chip and for quantity on a Qubit 2.0 Fluorometer (Agilent Technologies) using the RNA HS Assay Kit.

Article Title: Novel genes associated with enhanced motility of Escherichia coli ST131
Article Snippet: The PCR enrichment step was run using index primer 1 (one index per sample) and a custom transposon specific primer 4844 ( 5’-AATGATACGGCGACCACCGAGATCTACACTAGATCGCaacttcggaataggaactaagg-3’ ) to enrich for transposon insertion sites and allow for multiplexing sequencing; the thermocycler program is 72°C for 3 minutes, 98°C for 30 seconds followed by 22 cycles of 98°C for 10 seconds, 63°C for 30 seconds and 72°C for 1 minute. .. Verification and quantification of resulting libraries were calculated using a Qubit® 2.0 Fluorometer, 2100 Bioanalyser (Agilent Technologies) and qPCR (KAPA Biosciences).

Binding Assay:

Article Title: Full-Length Transcriptome Survey and Expression Analysis of Cassia obtusifolia to Discover Putative Genes Related to Aurantio-Obtusin Biosynthesis, Seed Formation and Development, and Stress Response
Article Snippet: Poly (A) RNA was isolated from the total RNA using the oligo (dT) magnetic bead binding method and the Poly (A) PuristTM Kit. .. The concentration and quality of the cDNA library were measured using Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer, respectively.

Article Title: Evaluating hybridization capture with RAD probes as a tool for museum genomics with historical bird specimens. Evaluating hybridization capture with RAD probes as a tool for museum genomics with historical bird specimens
Article Snippet: We used a 1–1.5× ratio of AmPure XP beads to remove small DNA fragments throughout the protocol and assessed DNA quantity and quality with a Qubit 2.0 fluorometer and an Agilent 2100 Bioanalyzer between all major steps. .. To perform hybridization capture reactions, we incubated each pool of samples with 250 ng of biotinylated probe for 72 hr at 55°C in a solution containing 20× saline‐sodium citrate (SSC), 50× Denhardt's Solution, 0.5 mol/L EDTA, 10% sodium dodecyl sulfate (SDS), and a blockers mix containing Chicken Hybloc (0.5 μg/μl), IDT's xGen Universal Blocking Oligo ‐TSHT‐ i5 (0.05 nmol/μl), and IDT's xGen Universal Blocking Oligo ‐TSHT‐ i7 (0.05 nmol/μl).

Molecular Weight:

Article Title: Evaluating hybridization capture with RAD probes as a tool for museum genomics with historical bird specimens. Evaluating hybridization capture with RAD probes as a tool for museum genomics with historical bird specimens
Article Snippet: To produce whole genome libraries, we sheared high molecular weight DNA from modern tissue samples to ~400 bp using a M‐220 Focused‐ultrasonicator (Covaris). .. We used a 1–1.5× ratio of AmPure XP beads to remove small DNA fragments throughout the protocol and assessed DNA quantity and quality with a Qubit 2.0 fluorometer and an Agilent 2100 Bioanalyzer between all major steps.

Multiplexing:

Article Title: Genome-Wide Discovery of Genes Required for Capsule Production by Uropathogenic Escherichia coli
Article Snippet: The PCR enrichment step, for which we used a custom transposon-specific primer to enrich for transposon insertion sites and an index primer (one index per sample) to allow for multiplexing sequencing, was performed at 72°C for 3 min and 98°C for 30 s, followed by 22 cycles of 98°C for 10 s, 63°C for 30 s, and 72°C for 1 min. Each library was purified using Agencourt Ampure XP magnetic beads. .. Library verification and quantification were undertaken using a Qubit 2.0 fluorometer and 4200 Tapestation system (Agilent Technologies).

Article Title: Novel genes associated with enhanced motility of Escherichia coli ST131
Article Snippet: The PCR enrichment step was run using index primer 1 (one index per sample) and a custom transposon specific primer 4844 ( 5’-AATGATACGGCGACCACCGAGATCTACACTAGATCGCaacttcggaataggaactaagg-3’ ) to enrich for transposon insertion sites and allow for multiplexing sequencing; the thermocycler program is 72°C for 3 minutes, 98°C for 30 seconds followed by 22 cycles of 98°C for 10 seconds, 63°C for 30 seconds and 72°C for 1 minute. .. Verification and quantification of resulting libraries were calculated using a Qubit® 2.0 Fluorometer, 2100 Bioanalyser (Agilent Technologies) and qPCR (KAPA Biosciences).

RNA Sequencing Assay:

Article Title: Endogenous Reprogramming of Alpha Cells into Beta Cells Induced by Viral Gene Therapy Reverses Autoimmune Diabetes
Article Snippet: Paragraph title: RNA Sequencing (RNA-seq) and analysis ... Each sample was assessed using Qubit 2.0 fluorometer and Agilent TapeStation 2200 for RNA quantity and quality.

Article Title: MYH9 binds to lncRNA gene PTCSC2 and regulates FOXE1 in the 9q22 thyroid cancer risk locus
Article Snippet: Paragraph title: RNA-Seq Sample Preparation and Detection. ... RNA concentration was determined by using Qubit 2.0 Fluorometer (Agilent Technologies) with an RNA HS Assay Kit.

Article Title: Murine models of Pneumocystis infection recapitulate human primary immune disorders
Article Snippet: Paragraph title: RNA-seq. ... RNA quantity and quality was assessed using Qubit 2.0 fluorometer and Agilent Bioanalyzer TapeStation 2200.

Article Title: Comprehensive Transcriptome Analysis Reveals Competing Endogenous RNA Networks During Avian Leukosis Virus, Subgroup J-Induced Tumorigenesis in Chickens
Article Snippet: For small RNA-Seq, small RNA fractions were ligated to 5′ and 3′ RNA adaptors. .. Subsequently, reverse transcription and PCR were conducted to construct the cDNA library (150 bp), and then the libraries underwent quantification and quality assessment using a Qubit® 2.0 fluorometer and an Agilent 2100 Bioanalyzer (Agilent).

Article Title: Prognostic and biological significance of the proangiogenic factor EGFL7 in acute myeloid leukemia
Article Snippet: In the cohort of younger adults with CN-AML ( n = 374) transcriptome analysis was performed with total RNA sequencing. .. In brief, extracted total RNA was assessed for quality on an Agilent 2100 Bioanalyzer (BioA) using the RNA 6000 Nano chip and for quantity on a Qubit 2.0 Fluorometer (Agilent Technologies) using the RNA HS Assay Kit.

RNA HS Assay:

Article Title: MYH9 binds to lncRNA gene PTCSC2 and regulates FOXE1 in the 9q22 thyroid cancer risk locus
Article Snippet: The total RNA samples for RNA-seq were extracted by TRIzol reagent (Invitrogen) and then treated by DNase-I (Ambion) to eliminate DNA contamination. .. RNA concentration was determined by using Qubit 2.0 Fluorometer (Agilent Technologies) with an RNA HS Assay Kit. .. The integrity of the RNA samples was assessed by BioAnalyzer (Agilent).

Article Title: Prognostic and biological significance of the proangiogenic factor EGFL7 in acute myeloid leukemia
Article Snippet: In the cohort of younger adults with CN-AML ( n = 374) transcriptome analysis was performed with total RNA sequencing. .. In brief, extracted total RNA was assessed for quality on an Agilent 2100 Bioanalyzer (BioA) using the RNA 6000 Nano chip and for quantity on a Qubit 2.0 Fluorometer (Agilent Technologies) using the RNA HS Assay Kit. .. Samples with a RNA integrity number (RIN) greater than four, with no visible sign of genomic DNA (gDNA) contamination and a concentration of > 40 ng/μL were used for total RNA library generation.

Magnetic Beads:

Article Title: Peptide vaccine immunotherapy biomarkers and response patterns in pediatric gliomas
Article Snippet: Each sample was assessed using a Qubit 2.0 fluorometer and Agilent 4 for RNA quantity and quality. .. Total RNA libraries were generated using the Illumina TruSeq Stranded mRNA (Poly-A+) sample preparation kit.

Article Title: Transcriptomic profiles of human foreskin fibroblast cells in response to orf virus
Article Snippet: Oligo-dT coated magnetic beads were used to isolate the poly-A containing mRNA molecules. .. The quantification and qualification of the library were carried out using Qubit® 2.0 Fluorometer and Agilent Bioanalyzer 2100 (con.=3.5-5.8ng/μL, peak length.=347-367 bp).

Article Title: Genome-Wide Discovery of Genes Required for Capsule Production by Uropathogenic Escherichia coli
Article Snippet: The PCR enrichment step, for which we used a custom transposon-specific primer to enrich for transposon insertion sites and an index primer (one index per sample) to allow for multiplexing sequencing, was performed at 72°C for 3 min and 98°C for 30 s, followed by 22 cycles of 98°C for 10 s, 63°C for 30 s, and 72°C for 1 min. Each library was purified using Agencourt Ampure XP magnetic beads. .. Library verification and quantification were undertaken using a Qubit 2.0 fluorometer and 4200 Tapestation system (Agilent Technologies).

Article Title: Novel genes associated with enhanced motility of Escherichia coli ST131
Article Snippet: Each library was purified using Agencourt® Ampure® XP magnetic beads. .. Verification and quantification of resulting libraries were calculated using a Qubit® 2.0 Fluorometer, 2100 Bioanalyser (Agilent Technologies) and qPCR (KAPA Biosciences).

Isolation:

Article Title: Endogenous Reprogramming of Alpha Cells into Beta Cells Induced by Viral Gene Therapy Reverses Autoimmune Diabetes
Article Snippet: Total RNA was extracted from alpha-cell-derived INS+ cells (Neo INS+) that were isolated 1 month after AAV-PM infusion in ALX-treated GCG-Cre; R26RTomato ; MIP-GFP mice, based on expression of tomato red (alpha-cell lineage) and GFP by flow cytometry. .. Each sample was assessed using Qubit 2.0 fluorometer and Agilent TapeStation 2200 for RNA quantity and quality.

Article Title: Peptide vaccine immunotherapy biomarkers and response patterns in pediatric gliomas
Article Snippet: Paragraph title: RNA isolation and library prep. ... Each sample was assessed using a Qubit 2.0 fluorometer and Agilent 4 for RNA quantity and quality.

Article Title: Transcriptome analyses provide insights into the difference of alkaloids biosynthesis in the Chinese goldthread (Coptis chinensis Franch.) from different biotopes
Article Snippet: Paragraph title: RNA isolation, cDNA library preparation and Illumina sequencing ... After library construction, the concentration and insert size of the three libraries were tested using Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer, respectively.

Article Title: Landscape of the genome and host cell response of Mycobacterium shigaense reveals pathogenic features
Article Snippet: Paragraph title: RNA isolation, QC and sequencing ... RNA quantity and quality were estimated with a Qubit 2.0 Fluorometer and Agilent Technology 2100 Bioanalyzer, respectively.

Article Title: Sequencing and De Novo Assembly of the Asian Clam (Corbicula fluminea) Transcriptome Using the Illumina GAIIx Method
Article Snippet: Paragraph title: RNA isolation and Illumina sequencing ... The total RNA concentration and quality were determined and quantified using a QUBIT 2.0 fluorometer and an Agilent 2100 BioAnalyzer.

Article Title: Full-Length Transcriptome Survey and Expression Analysis of Cassia obtusifolia to Discover Putative Genes Related to Aurantio-Obtusin Biosynthesis, Seed Formation and Development, and Stress Response
Article Snippet: Isolated poly (A) RNA was eluted with 20 μL of RNase-free water. .. The concentration and quality of the cDNA library were measured using Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer, respectively.

RNA Extraction:

Article Title: Transcriptomic profiles of human foreskin fibroblast cells in response to orf virus
Article Snippet: Paragraph title: Cell cultures, RNA extraction, mRNA-Seq ... The quantification and qualification of the library were carried out using Qubit® 2.0 Fluorometer and Agilent Bioanalyzer 2100 (con.=3.5-5.8ng/μL, peak length.=347-367 bp).

Mouse Assay:

Article Title: Endogenous Reprogramming of Alpha Cells into Beta Cells Induced by Viral Gene Therapy Reverses Autoimmune Diabetes
Article Snippet: Sorted normal GFP+ beta cells (Beta) and normal TOM+ alpha cells (Alpha) from GCG-Cre; R26RTomato ; MIP-GFP mice without any treatment were used as controls. .. Each sample was assessed using Qubit 2.0 fluorometer and Agilent TapeStation 2200 for RNA quantity and quality.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Evaluating hybridization capture with RAD probes as a tool for museum genomics with historical bird specimens. Evaluating hybridization capture with RAD probes as a tool for museum genomics with historical bird specimens
Article Snippet: We used a 1–1.5× ratio of AmPure XP beads to remove small DNA fragments throughout the protocol and assessed DNA quantity and quality with a Qubit 2.0 fluorometer and an Agilent 2100 Bioanalyzer between all major steps. .. We used a 1–1.5× ratio of AmPure XP beads to remove small DNA fragments throughout the protocol and assessed DNA quantity and quality with a Qubit 2.0 fluorometer and an Agilent 2100 Bioanalyzer between all major steps.

cDNA Library Assay:

Article Title: Endogenous Reprogramming of Alpha Cells into Beta Cells Induced by Viral Gene Therapy Reverses Autoimmune Diabetes
Article Snippet: Each sample was assessed using Qubit 2.0 fluorometer and Agilent TapeStation 2200 for RNA quantity and quality. .. Each sample was assessed using Qubit 2.0 fluorometer and Agilent TapeStation 2200 for RNA quantity and quality.

Article Title: Transcriptome analyses provide insights into the difference of alkaloids biosynthesis in the Chinese goldthread (Coptis chinensis Franch.) from different biotopes
Article Snippet: Paragraph title: RNA isolation, cDNA library preparation and Illumina sequencing ... After library construction, the concentration and insert size of the three libraries were tested using Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer, respectively.

Article Title: Transcriptomic profiles of human foreskin fibroblast cells in response to orf virus
Article Snippet: The quantification and qualification of the library were carried out using Qubit® 2.0 Fluorometer and Agilent Bioanalyzer 2100 (con.=3.5-5.8ng/μL, peak length.=347-367 bp). .. The quantification and qualification of the library were carried out using Qubit® 2.0 Fluorometer and Agilent Bioanalyzer 2100 (con.=3.5-5.8ng/μL, peak length.=347-367 bp).

Article Title: Comprehensive Transcriptome Analysis Reveals Competing Endogenous RNA Networks During Avian Leukosis Virus, Subgroup J-Induced Tumorigenesis in Chickens
Article Snippet: For small RNA-Seq, small RNA fractions were ligated to 5′ and 3′ RNA adaptors. .. Subsequently, reverse transcription and PCR were conducted to construct the cDNA library (150 bp), and then the libraries underwent quantification and quality assessment using a Qubit® 2.0 fluorometer and an Agilent 2100 Bioanalyzer (Agilent). .. Finally, the libraries were sequenced on an Illumina HiSeqTM 2500 instrument (Illumina).

Article Title: Full-Length Transcriptome Survey and Expression Analysis of Cassia obtusifolia to Discover Putative Genes Related to Aurantio-Obtusin Biosynthesis, Seed Formation and Development, and Stress Response
Article Snippet: The cDNA products were then subjected to construction of SMRTbell Template libraries using SMRTBell Template Prep Kit. .. The concentration and quality of the cDNA library were measured using Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer, respectively. .. Finally, a total of four SMRT cells were sequenced on PacBio RS II platform (Pacific Biosciences, Menlo Park, CA, USA).

Article Title: De novo Transcriptome Analysis and Molecular Marker Development of Two Hemarthria Species
Article Snippet: Paragraph title: cDNA library construction and illumina sequencing ... To ensure that the quality of the library was sufficient for sequencing, the concentration and insert size of the library was detected using a Qubit® 2.0 Fluorometer and Agilent 2100 Bioanalyzer, respectively.

Purification:

Article Title: Endogenous Reprogramming of Alpha Cells into Beta Cells Induced by Viral Gene Therapy Reverses Autoimmune Diabetes
Article Snippet: Each sample was assessed using Qubit 2.0 fluorometer and Agilent TapeStation 2200 for RNA quantity and quality. .. Each sample was assessed using Qubit 2.0 fluorometer and Agilent TapeStation 2200 for RNA quantity and quality.

Article Title: Peptide vaccine immunotherapy biomarkers and response patterns in pediatric gliomas
Article Snippet: Each sample was assessed using a Qubit 2.0 fluorometer and Agilent 4 for RNA quantity and quality. .. The first step in the workflow was purifying the Poly-A–containing mRNA molecules using Poly-T oligo-attached magnetic beads.

Article Title: Gut Microbiota-Regulated Pharmacokinetics of Berberine and Active Metabolites in Beagle Dogs After Oral Administration
Article Snippet: Then, the mixture of PCR products was purified with a GeneJET Gel Extraction Kit. .. The library quality was assessed on a Qubit 2.0 Fluorometer and Agilent Bioanalyzer 2100 system.

Article Title: MYH9 binds to lncRNA gene PTCSC2 and regulates FOXE1 in the 9q22 thyroid cancer risk locus
Article Snippet: RNA concentration was determined by using Qubit 2.0 Fluorometer (Agilent Technologies) with an RNA HS Assay Kit. .. RNA concentration was determined by using Qubit 2.0 Fluorometer (Agilent Technologies) with an RNA HS Assay Kit.

Article Title: Transcriptome analyses provide insights into the difference of alkaloids biosynthesis in the Chinese goldthread (Coptis chinensis Franch.) from different biotopes
Article Snippet: Then, ends of the purified cDNA were repaired and poly (A) was added. .. After library construction, the concentration and insert size of the three libraries were tested using Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer, respectively.

Article Title: Transcriptomic profiles of human foreskin fibroblast cells in response to orf virus
Article Snippet: The quantification and qualification of the library were carried out using Qubit® 2.0 Fluorometer and Agilent Bioanalyzer 2100 (con.=3.5-5.8ng/μL, peak length.=347-367 bp). .. The quantification and qualification of the library were carried out using Qubit® 2.0 Fluorometer and Agilent Bioanalyzer 2100 (con.=3.5-5.8ng/μL, peak length.=347-367 bp).

Article Title: Genome-Wide Discovery of Genes Required for Capsule Production by Uropathogenic Escherichia coli
Article Snippet: The PCR enrichment step, for which we used a custom transposon-specific primer to enrich for transposon insertion sites and an index primer (one index per sample) to allow for multiplexing sequencing, was performed at 72°C for 3 min and 98°C for 30 s, followed by 22 cycles of 98°C for 10 s, 63°C for 30 s, and 72°C for 1 min. Each library was purified using Agencourt Ampure XP magnetic beads. .. Library verification and quantification were undertaken using a Qubit 2.0 fluorometer and 4200 Tapestation system (Agilent Technologies).

Article Title: Sequencing and De Novo Assembly of the Asian Clam (Corbicula fluminea) Transcriptome Using the Illumina GAIIx Method
Article Snippet: Total RNA was extracted from these samples using TRIzol reagent (Invitrogen) followed by RNA purification with the RNeasy MiniElute Cleanup Kit (Qiagen) according to the manufacturer’s protocol. .. The total RNA concentration and quality were determined and quantified using a QUBIT 2.0 fluorometer and an Agilent 2100 BioAnalyzer.

Article Title: Comprehensive Transcriptome Analysis Reveals Competing Endogenous RNA Networks During Avian Leukosis Virus, Subgroup J-Induced Tumorigenesis in Chickens
Article Snippet: Thereafter, purification, end repair, and Illumina adaptor ligation of the cDNA were performed. .. Subsequently, reverse transcription and PCR were conducted to construct the cDNA library (150 bp), and then the libraries underwent quantification and quality assessment using a Qubit® 2.0 fluorometer and an Agilent 2100 Bioanalyzer (Agilent).

Article Title: Full-Length Transcriptome Survey and Expression Analysis of Cassia obtusifolia to Discover Putative Genes Related to Aurantio-Obtusin Biosynthesis, Seed Formation and Development, and Stress Response
Article Snippet: After PCR amplification, quality control, and purification, we performed size selection using BluePippin Size Selection System protocol and herein produced three fractions containing fragments of 1–2, 2–3, and 3–6 kb in length, respectively. .. The concentration and quality of the cDNA library were measured using Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer, respectively.

Article Title: Transcriptome analysis of genes involved in defense against alkaline stress in roots of wild jujube (Ziziphus acidojujuba)
Article Snippet: The enriched cDNA templates that were 150 nucleotides (nt) long were purified and used for further analysis. .. The purity and size of the libraries were checked by Qubit 2.0 Fluorometer and Agilent 2100 Bioanalyzer (Agilent Technologies).

Article Title: De novo Transcriptome Analysis and Molecular Marker Development of Two Hemarthria Species
Article Snippet: Next, PCR amplification was then performed using the Phusion High-Fidelity DNA polymerase in order to enrich the purified cDNA template. .. To ensure that the quality of the library was sufficient for sequencing, the concentration and insert size of the library was detected using a Qubit® 2.0 Fluorometer and Agilent 2100 Bioanalyzer, respectively.

Article Title: Novel genes associated with enhanced motility of Escherichia coli ST131
Article Snippet: Each library was purified using Agencourt® Ampure® XP magnetic beads. .. Verification and quantification of resulting libraries were calculated using a Qubit® 2.0 Fluorometer, 2100 Bioanalyser (Agilent Technologies) and qPCR (KAPA Biosciences).

Chromatin Immunoprecipitation:

Article Title: Prognostic and biological significance of the proangiogenic factor EGFL7 in acute myeloid leukemia
Article Snippet: In the cohort of younger adults with CN-AML ( n = 374) transcriptome analysis was performed with total RNA sequencing. .. In brief, extracted total RNA was assessed for quality on an Agilent 2100 Bioanalyzer (BioA) using the RNA 6000 Nano chip and for quantity on a Qubit 2.0 Fluorometer (Agilent Technologies) using the RNA HS Assay Kit. .. Samples with a RNA integrity number (RIN) greater than four, with no visible sign of genomic DNA (gDNA) contamination and a concentration of > 40 ng/μL were used for total RNA library generation.

Software:

Article Title: Gut Microbiota-Regulated Pharmacokinetics of Berberine and Active Metabolites in Beagle Dogs After Oral Administration
Article Snippet: The library quality was assessed on a Qubit 2.0 Fluorometer and Agilent Bioanalyzer 2100 system. .. Finally, the library was sequenced on a HiSeq2500 platform and 250 bp paired-end reads were generated.

Multiplex Assay:

Article Title: Genome-Wide Discovery of Genes Required for Capsule Production by Uropathogenic Escherichia coli
Article Snippet: Paragraph title: Multiplex TraDIS. ... Library verification and quantification were undertaken using a Qubit 2.0 fluorometer and 4200 Tapestation system (Agilent Technologies).

Article Title: Transcriptome analysis of genes involved in defense against alkaline stress in roots of wild jujube (Ziziphus acidojujuba)
Article Snippet: The purity and size of the libraries were checked by Qubit 2.0 Fluorometer and Agilent 2100 Bioanalyzer (Agilent Technologies). .. Finally, the adapter-ligated cDNA fragment libraries were run on an Illumina GAIIx analyzer to complete the cluster generation and primer hybridization.

Selection:

Article Title: Transcriptome analyses provide insights into the difference of alkaloids biosynthesis in the Chinese goldthread (Coptis chinensis Franch.) from different biotopes
Article Snippet: This was followed by the selection of suitable fragments using AMPure XP beads. .. After library construction, the concentration and insert size of the three libraries were tested using Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer, respectively.

Article Title: Full-Length Transcriptome Survey and Expression Analysis of Cassia obtusifolia to Discover Putative Genes Related to Aurantio-Obtusin Biosynthesis, Seed Formation and Development, and Stress Response
Article Snippet: After PCR amplification, quality control, and purification, we performed size selection using BluePippin Size Selection System protocol and herein produced three fractions containing fragments of 1–2, 2–3, and 3–6 kb in length, respectively. .. The concentration and quality of the cDNA library were measured using Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer, respectively.

Sample Prep:

Article Title: Peptide vaccine immunotherapy biomarkers and response patterns in pediatric gliomas
Article Snippet: Each sample was assessed using a Qubit 2.0 fluorometer and Agilent 4 for RNA quantity and quality. .. Each sample was assessed using a Qubit 2.0 fluorometer and Agilent 4 for RNA quantity and quality.

Article Title: MYH9 binds to lncRNA gene PTCSC2 and regulates FOXE1 in the 9q22 thyroid cancer risk locus
Article Snippet: Paragraph title: RNA-Seq Sample Preparation and Detection. ... RNA concentration was determined by using Qubit 2.0 Fluorometer (Agilent Technologies) with an RNA HS Assay Kit.

Article Title: Responses of intestinal virome to silver nanoparticles: safety assessment by classical virology, whole-genome sequencing and bioinformatics approaches
Article Snippet: Fragmented DNA was used for preparing uniquely indexed sample library using the TruSeq DNA PCR-Free Sample Preparation kit (Illumina), as recommended by the manufacturer and the final libraries were quantified with Qubit and 2100 Bioanalyzer. .. The size selected final libraries were quantified with Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer.

Article Title: Sequencing and De Novo Assembly of the Asian Clam (Corbicula fluminea) Transcriptome Using the Illumina GAIIx Method
Article Snippet: The total RNA concentration and quality were determined and quantified using a QUBIT 2.0 fluorometer and an Agilent 2100 BioAnalyzer. .. The total RNA concentration and quality were determined and quantified using a QUBIT 2.0 fluorometer and an Agilent 2100 BioAnalyzer.

Article Title: Prognostic and biological significance of the proangiogenic factor EGFL7 in acute myeloid leukemia
Article Snippet: In brief, extracted total RNA was assessed for quality on an Agilent 2100 Bioanalyzer (BioA) using the RNA 6000 Nano chip and for quantity on a Qubit 2.0 Fluorometer (Agilent Technologies) using the RNA HS Assay Kit. .. Samples with a RNA integrity number (RIN) greater than four, with no visible sign of genomic DNA (gDNA) contamination and a concentration of > 40 ng/μL were used for total RNA library generation.

Next-Generation Sequencing:

Article Title: Responses of intestinal virome to silver nanoparticles: safety assessment by classical virology, whole-genome sequencing and bioinformatics approaches
Article Snippet: After quantification of the products, equal concentrations of the amplified DNA from each experimental group were sequenced using next-generation sequencing platforms. .. The size selected final libraries were quantified with Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer.

Ancient DNA Assay:

Article Title: Evaluating hybridization capture with RAD probes as a tool for museum genomics with historical bird specimens. Evaluating hybridization capture with RAD probes as a tool for museum genomics with historical bird specimens
Article Snippet: After quantifying DNA content in each sample, we made standardized dilutions of each sample and combined equal amounts of these dilutions to create one pool of modern DNA samples (n = 6) and two pools of ancient DNA samples (n = 7 each). .. We used a 1–1.5× ratio of AmPure XP beads to remove small DNA fragments throughout the protocol and assessed DNA quantity and quality with a Qubit 2.0 fluorometer and an Agilent 2100 Bioanalyzer between all major steps.

Produced:

Article Title: Full-Length Transcriptome Survey and Expression Analysis of Cassia obtusifolia to Discover Putative Genes Related to Aurantio-Obtusin Biosynthesis, Seed Formation and Development, and Stress Response
Article Snippet: After PCR amplification, quality control, and purification, we performed size selection using BluePippin Size Selection System protocol and herein produced three fractions containing fragments of 1–2, 2–3, and 3–6 kb in length, respectively. .. The concentration and quality of the cDNA library were measured using Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer, respectively.

Concentration Assay:

Article Title: MYH9 binds to lncRNA gene PTCSC2 and regulates FOXE1 in the 9q22 thyroid cancer risk locus
Article Snippet: The total RNA samples for RNA-seq were extracted by TRIzol reagent (Invitrogen) and then treated by DNase-I (Ambion) to eliminate DNA contamination. .. RNA concentration was determined by using Qubit 2.0 Fluorometer (Agilent Technologies) with an RNA HS Assay Kit. .. The integrity of the RNA samples was assessed by BioAnalyzer (Agilent).

Article Title: Transcriptome analyses provide insights into the difference of alkaloids biosynthesis in the Chinese goldthread (Coptis chinensis Franch.) from different biotopes
Article Snippet: PCR amplifications of fragments were performed to create final cDNA libraries for sequencing. .. After library construction, the concentration and insert size of the three libraries were tested using Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer, respectively. .. The valid concentration of the three libraries was accurately quantified with Q-PCR method to ensure reliability.

Article Title: Murine models of Pneumocystis infection recapitulate human primary immune disorders
Article Snippet: RNA quantity and quality was assessed using Qubit 2.0 fluorometer and Agilent Bioanalyzer TapeStation 2200. .. Illumina TruSeq Stranded mRNA sample prep kit was used for library preparation, followed by validation with KAPA Biosystems primer premix kit with Illumina-compatible DNA primers and Qubit 2.0 fluorometer, as previously described ( ).

Article Title: Sequencing and De Novo Assembly of the Asian Clam (Corbicula fluminea) Transcriptome Using the Illumina GAIIx Method
Article Snippet: Total RNA was extracted from these samples using TRIzol reagent (Invitrogen) followed by RNA purification with the RNeasy MiniElute Cleanup Kit (Qiagen) according to the manufacturer’s protocol. .. The total RNA concentration and quality were determined and quantified using a QUBIT 2.0 fluorometer and an Agilent 2100 BioAnalyzer. .. A total RNA sample of approximately 0.1-0.4μg with RIN ≥8 and a 260/280 nm absorption ratio ≥1.8 was obtained.

Article Title: Full-Length Transcriptome Survey and Expression Analysis of Cassia obtusifolia to Discover Putative Genes Related to Aurantio-Obtusin Biosynthesis, Seed Formation and Development, and Stress Response
Article Snippet: The cDNA products were then subjected to construction of SMRTbell Template libraries using SMRTBell Template Prep Kit. .. The concentration and quality of the cDNA library were measured using Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer, respectively. .. Finally, a total of four SMRT cells were sequenced on PacBio RS II platform (Pacific Biosciences, Menlo Park, CA, USA).

Article Title: De novo Transcriptome Analysis and Molecular Marker Development of Two Hemarthria Species
Article Snippet: Next, PCR amplification was then performed using the Phusion High-Fidelity DNA polymerase in order to enrich the purified cDNA template. .. To ensure that the quality of the library was sufficient for sequencing, the concentration and insert size of the library was detected using a Qubit® 2.0 Fluorometer and Agilent 2100 Bioanalyzer, respectively. .. Additionally, the effective concentration of the library was accurately quantified using quantitative PCR (Q-PCR).

High Throughput Screening Assay:

Article Title: Comprehensive Transcriptome Analysis Reveals Competing Endogenous RNA Networks During Avian Leukosis Virus, Subgroup J-Induced Tumorigenesis in Chickens
Article Snippet: Paragraph title: High-Throughput Sequencing ... Subsequently, reverse transcription and PCR were conducted to construct the cDNA library (150 bp), and then the libraries underwent quantification and quality assessment using a Qubit® 2.0 fluorometer and an Agilent 2100 Bioanalyzer (Agilent).

Gel Extraction:

Article Title: Gut Microbiota-Regulated Pharmacokinetics of Berberine and Active Metabolites in Beagle Dogs After Oral Administration
Article Snippet: Then, the mixture of PCR products was purified with a GeneJET Gel Extraction Kit. .. The library quality was assessed on a Qubit 2.0 Fluorometer and Agilent Bioanalyzer 2100 system.

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    Agilent technologies qubit 2 0 fluorometer
    Qubit 2 0 Fluorometer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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