qubit 2 0 fluorimeter  (Thermo Fisher)


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    Structured Review

    Thermo Fisher qubit 2 0 fluorimeter
    Qubit 2 0 Fluorimeter, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit 2 0 fluorimeter/product/Thermo Fisher
    Average 99 stars, based on 44 article reviews
    Price from $9.99 to $1999.99
    qubit 2 0 fluorimeter - by Bioz Stars, 2019-12
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Suppressor mutations in Rpf2–Rrs1 or Rpl5 bypass the Cgr1 function for pre-ribosomal 5S RNP-rotation
    Article Snippet: Following centrifugation for 3 min at 13,500 rpm, the supernatant was removed and the pellet was resuspended in 400 µl of TE buffer. .. The concentration of the genomic DNA was determined with a Qubit 2.0 fluorimeter (Invitrogen).

    Amplification:

    Article Title: Putting the Mess in Order: Aspergillus welwitschiae (and Not A. niger) Is the Etiological Agent of Sisal Bole Rot Disease in Brazil
    Article Snippet: The integrity and quantification of genomic DNA were evaluated by fluorimetry using the Qubit 2.0 Fluorimeter® (Invitrogen). .. The integrity and quantification of genomic DNA were evaluated by fluorimetry using the Qubit 2.0 Fluorimeter® (Invitrogen).

    Article Title: A Nosocomial Outbreak of Human Monkeypox in the Central African Republic
    Article Snippet: The monkeypox virus was isolated and amplified by 1 passage of intracranial inoculation in the brains of newborn mice using pus or scab homogenates from patients, as previously described [ ]. .. DNA was quantified using the Qubit dsDNA BR Assay kit with the Qubit 2.0 fluorimeter (Life Technologies, CA).

    Article Title: Extreme-Depth Re-sequencing of Mitochondrial DNA Finds No Evidence of Paternal Transmission in Humans
    Article Snippet: Initially, primer efficiency and specificity was assessed as successful after no amplification of DNA from rho0 cell lines, which contain no mtDNA. .. Amplified products were assessed by gel electrophoresis against DNA+ve and DNA-ve controls, and quantified using a Qubit 2.0 fluorimeter (Life Technologies, Paisley, UK). .. Each amplicon was individually purified using Agencourt AMPure XP beads (Beckman-Coulter, USA), pooled in equimolar concentrations and re-quantified.

    Article Title: Somatic mtDNA variation is an important component of Parkinson's disease
    Article Snippet: Initially, primer efficiency and specificity was assessed as successful after zero amplification of DNA from Rho0 cell lines, avoiding the unintended amplification of nuclear pseudogenes. .. Amplified products were assessed by gel electrophoresis, against DNA+ve and DNA−ve controls, and quantified using a Qubit 2.0 fluorimeter (Life Technologies, Paisley, UK). .. Each amplicon was individually purified using Agencourt AMPure XP beads (Beckman-Coulter, USA), pooled in equimolar concentrations and requantified.

    High Throughput Screening Assay:

    Article Title: Suppressor mutations in Rpf2–Rrs1 or Rpl5 bypass the Cgr1 function for pre-ribosomal 5S RNP-rotation
    Article Snippet: Paragraph title: Identification of suppressors by high-throughput sequencing ... The concentration of the genomic DNA was determined with a Qubit 2.0 fluorimeter (Invitrogen).

    Synthesized:

    Article Title: Transcriptome and secretome analysis of Aspergillus fumigatus in the presence of sugarcane bagasse
    Article Snippet: RNA integrity was confirmed with a bioanalyzer by using the “Agilent RNA 6000 Nano” kit (Agilent Technologies, Santa Clara, CA, EUA) and the “Plant Total RNA Nano” protocol. .. RNA was quantified on a Qubit® 2.0 fluorimeter (Thermo Fisher Scientific, Waltham, MS, EUA) with the Qubit® RNA BR Assay kit (Thermo Fisher Scientific, Waltham, MS, EUA). cDNA was synthesized from 1 μg of mRNA using SuperScript® II Reverse Transcriptase (Invitrogen, Carlsbad, CA, EUA). .. RNA sequencing libraries were prepared using the “TruSeq Stranded mRNA HT Sample Prep” kit (Illumina, San Diego, CA, EUA), mRNA enrichment was performed using magnetic beads coupled with oligo (dT).

    Incubation:

    Article Title: Suppressor mutations in Rpf2–Rrs1 or Rpl5 bypass the Cgr1 function for pre-ribosomal 5S RNP-rotation
    Article Snippet: To digest the RNA, 30 µl of a 1 mg/ml DNase-free RNase A solution (Sigma) was added and the tubes were incubated for 5 min at 37 °C. .. The concentration of the genomic DNA was determined with a Qubit 2.0 fluorimeter (Invitrogen).

    Article Title: Histone deacetylase class-I inhibition promotes epithelial gene expression in pancreatic cancer cells in a BRD4- and MYC-dependent manner
    Article Snippet: The following day, samples were incubated with Protein-A Sepharose (GE Healthcare) for 2 h at 4°C, immune complexes were washed, de-crosslinked and DNA was extracted. .. DNA was quantitated using the Qubit® dsDNA HS assay and a Qubit® 2.0 Fluorimeter (Invitrogen).

    Mass Spectrometry:

    Article Title: Transcriptome and secretome analysis of Aspergillus fumigatus in the presence of sugarcane bagasse
    Article Snippet: RNA integrity was confirmed with a bioanalyzer by using the “Agilent RNA 6000 Nano” kit (Agilent Technologies, Santa Clara, CA, EUA) and the “Plant Total RNA Nano” protocol. .. RNA was quantified on a Qubit® 2.0 fluorimeter (Thermo Fisher Scientific, Waltham, MS, EUA) with the Qubit® RNA BR Assay kit (Thermo Fisher Scientific, Waltham, MS, EUA). cDNA was synthesized from 1 μg of mRNA using SuperScript® II Reverse Transcriptase (Invitrogen, Carlsbad, CA, EUA). .. RNA sequencing libraries were prepared using the “TruSeq Stranded mRNA HT Sample Prep” kit (Illumina, San Diego, CA, EUA), mRNA enrichment was performed using magnetic beads coupled with oligo (dT).

    Gel Purification:

    Article Title: A lanthipeptide library used to identify a protein–protein interaction inhibitor
    Article Snippet: The PCR products (appearing as a strong band at 200-250 bp) were gel-purified with Qiagen gel purification kit. .. DNA concentration was quantified with a Qubit® 2.0 fluorimeter (Invitrogen) using a Qubit HS dsDNA HS assay kit.

    Countercurrent Chromatography:

    Article Title: Somatic mtDNA variation is an important component of Parkinson's disease
    Article Snippet: To eliminate the potential for error and nuclear DNA contamination, amplicons were polymerized using PrimeSTAR GXL DNA polymerase (error rate = 0.00108%, Takara Bio, Saint-Germain-en-Laye, France) in 2 overlapping fragments, using primer set-1: CCC TCT CTC CTA CTC CTG-F (m.6222–6239) and CAG GTG GTC AAG TAT TTA TGG–R (m.16133–16,153), and set-2: CAT CTT GCC CTT CAT TAT TGC–F (m.15295–15,315) and GGC AGG ATA GTT CAG ACG-R (7773–7791). .. Amplified products were assessed by gel electrophoresis, against DNA+ve and DNA−ve controls, and quantified using a Qubit 2.0 fluorimeter (Life Technologies, Paisley, UK).

    Flow Cytometry:

    Article Title: A lanthipeptide library used to identify a protein–protein interaction inhibitor
    Article Snippet: Nextera XT Index N701 and S517 (Nextera XT index kit FC-131-1001) were used to amplify the products of the first PCR using Phusion polymerase with 8 cycles of denaturing (94 °C for 30 s), annealing (66 °C for 1 min) and extending (72 °C for 30 s). .. DNA concentration was quantified with a Qubit® 2.0 fluorimeter (Invitrogen) using a Qubit HS dsDNA HS assay kit.

    Article Title: Somatic mtDNA variation is an important component of Parkinson's disease
    Article Snippet: To avoid run-specific amplification variation, case and control SN and FC samples were randomly mixed in each isolation and enrichment experiment. .. Amplified products were assessed by gel electrophoresis, against DNA+ve and DNA−ve controls, and quantified using a Qubit 2.0 fluorimeter (Life Technologies, Paisley, UK).

    Concentration Assay:

    Article Title: Suppressor mutations in Rpf2–Rrs1 or Rpl5 bypass the Cgr1 function for pre-ribosomal 5S RNP-rotation
    Article Snippet: To estimate the integrity of the isolated genomic DNA, 2.5 µl of the preparation was migrated on a 1% agarose gel. .. The concentration of the genomic DNA was determined with a Qubit 2.0 fluorimeter (Invitrogen). .. Libraries were generated from 1 µg of genomic DNA and high-throughput sequencing was performed on a HiSeq 3000 instrument (Illumina).

    Article Title: Bleomycin-induced genome structural variations in normal, non-tumor cells
    Article Snippet: Total DNA was isolated from tissue and cultured cells using the Quick-gDNATM MiniPrep kit (Zymo Research Corporation, Irvine, CA, USA) following the manufacturer’s instructions. .. DNA concentration was determined using the Qubit 2.0 fluorimeter (Life Technologies Corporation, Carlsbad, CA, USA). .. Long-range PCR was performed with the Takara LA PCR kit (Takara Bio Inc., Otsu, Shiga, Japan) according to the manufacturer's protocol.

    Article Title: A lanthipeptide library used to identify a protein–protein interaction inhibitor
    Article Snippet: The PCR products (appearing as a strong band at 200-250 bp) were gel-purified with Qiagen gel purification kit. .. DNA concentration was quantified with a Qubit® 2.0 fluorimeter (Invitrogen) using a Qubit HS dsDNA HS assay kit. .. Deep sequencing was performed in the Roy J.

    Article Title: Prednisolone induces osteoporosis-like phenotypes via focal adhesion signaling pathway in zebrafish larvae
    Article Snippet: RNA purity was assessed using the NanoPhotome spectrophotometer (IMPLEN, West Lake Village, CA, USA). .. RNA concentration was measured using the Qubit® RNA Assay Kit in a Qubit® 2.0 Fluorimeter (Life Technologies). .. RNA integrity was assessed using the RNA Nano 6000 Assay Kit with the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: Somatic mtDNA variation is an important component of Parkinson's disease
    Article Snippet: Amplified products were assessed by gel electrophoresis, against DNA+ve and DNA−ve controls, and quantified using a Qubit 2.0 fluorimeter (Life Technologies, Paisley, UK). .. Each amplicon was individually purified using Agencourt AMPure XP beads (Beckman-Coulter, USA), pooled in equimolar concentrations and requantified.

    Article Title: The human CIB1–EVER1–EVER2 complex governs keratinocyte-intrinsic immunity to β-papillomaviruses
    Article Snippet: For kindred F, DNA was extracted from peripheral blood samples by the salting out method. .. DNA concentration was measured with a Qubit 2.0 fluorimeter (Thermo Fisher Scientific). .. WES was performed for probands and affected family members.

    Cell Culture:

    Article Title: Bleomycin-induced genome structural variations in normal, non-tumor cells
    Article Snippet: Total DNA was isolated from tissue and cultured cells using the Quick-gDNATM MiniPrep kit (Zymo Research Corporation, Irvine, CA, USA) following the manufacturer’s instructions. .. DNA concentration was determined using the Qubit 2.0 fluorimeter (Life Technologies Corporation, Carlsbad, CA, USA).

    Sequencing:

    Article Title: Suppressor mutations in Rpf2–Rrs1 or Rpl5 bypass the Cgr1 function for pre-ribosomal 5S RNP-rotation
    Article Snippet: Paragraph title: Identification of suppressors by high-throughput sequencing ... The concentration of the genomic DNA was determined with a Qubit 2.0 fluorimeter (Invitrogen).

    Article Title: Histone deacetylase class-I inhibition promotes epithelial gene expression in pancreatic cancer cells in a BRD4- and MYC-dependent manner
    Article Snippet: DNA was quantitated using the Qubit® dsDNA HS assay and a Qubit® 2.0 Fluorimeter (Invitrogen). .. DNA was quantitated using the Qubit® dsDNA HS assay and a Qubit® 2.0 Fluorimeter (Invitrogen).

    Article Title: A lanthipeptide library used to identify a protein–protein interaction inhibitor
    Article Snippet: Paragraph title: Sequencing of the plasmid library ... DNA concentration was quantified with a Qubit® 2.0 fluorimeter (Invitrogen) using a Qubit HS dsDNA HS assay kit.

    Article Title: Prednisolone induces osteoporosis-like phenotypes via focal adhesion signaling pathway in zebrafish larvae
    Article Snippet: Paragraph title: Deep sequencing analysis ... RNA concentration was measured using the Qubit® RNA Assay Kit in a Qubit® 2.0 Fluorimeter (Life Technologies).

    Article Title: The human CIB1–EVER1–EVER2 complex governs keratinocyte-intrinsic immunity to β-papillomaviruses
    Article Snippet: Sequencing was performed on an Illumina Genome Analyzer IIx (Illumina) generating 72- or 100-base reads. .. DNA concentration was measured with a Qubit 2.0 fluorimeter (Thermo Fisher Scientific).

    Article Title: Impact of demographics on human gut microbial diversity in a US Midwest population
    Article Snippet: Paragraph title: Sample preparation and sequencing ... Final quantitation was performed using a QuBit HS dsDNA kit and the QuBit 2.0 fluorimeter (Life Technologies, Carlsbad, CA, USA).

    Sonication:

    Article Title: Histone deacetylase class-I inhibition promotes epithelial gene expression in pancreatic cancer cells in a BRD4- and MYC-dependent manner
    Article Snippet: Prior to library preparation, DNA samples were sonicated again for 40 cycles (total volume 10 μl) to ensure fragment sizes of ≤300 bp. .. DNA was quantitated using the Qubit® dsDNA HS assay and a Qubit® 2.0 Fluorimeter (Invitrogen).

    DNA Extraction:

    Article Title: Whole-genome sequencing reveals principles of brain retrotransposition in neurodevelopmental disorders
    Article Snippet: Paragraph title: DNA extraction ... The yield and the quality of extracted DNA were assayed using a NanoDrop and a Qubit 2.0 Fluorimeter (Invitrogen, dsDNA HS Assay, cat ).

    Article Title: Bleomycin-induced genome structural variations in normal, non-tumor cells
    Article Snippet: Paragraph title: DNA isolation and quantification ... DNA concentration was determined using the Qubit 2.0 fluorimeter (Life Technologies Corporation, Carlsbad, CA, USA).

    Article Title: Human papillomavirus detection using the Abbott RealTime high-risk HPV tests compared with conventional nested PCR coupled to high-throughput sequencing of amplification products in cervical smear specimens from a Gabonese female population
    Article Snippet: Paragraph title: DNA extraction from liquid-based cytology samples ... DNA was quantified using the Qubit® dsDNA BR Assay kit with the Qubit 2.0 fluorimeter (Life Technologies, Carlsbad, CA, USA) primarily to confirm the previous DNA purification step.

    Article Title: Putting the Mess in Order: Aspergillus welwitschiae (and Not A. niger) Is the Etiological Agent of Sisal Bole Rot Disease in Brazil
    Article Snippet: Genomic DNA was extracted using the UltraClean® Microbial DNA Isolation kit (Mo Bio, CA, USA). .. The integrity and quantification of genomic DNA were evaluated by fluorimetry using the Qubit 2.0 Fluorimeter® (Invitrogen).

    Article Title: A Nosocomial Outbreak of Human Monkeypox in the Central African Republic
    Article Snippet: Paragraph title: Isolation of the Monkeypox Virus, DNA Extraction, and Molecular Assays ... DNA was quantified using the Qubit dsDNA BR Assay kit with the Qubit 2.0 fluorimeter (Life Technologies, CA).

    Article Title: Molecular analysis of human Papillomavirus detected among women positive for cervical lesions by visual inspection with acetic acid/Lugol’s iodine (VIA/VILI) in Libreville, Gabon
    Article Snippet: Paragraph title: DNA extraction ... DNA was quantified using Qubit® dsDNA BR Assay kit with the Qubit 2.0 fluorimeter (Life Technologies, CA, USA).

    Article Title: Linking rhizosphere microbiome composition of wild and domesticated Phaseolus vulgaris to genotypic and root phenotypic traits
    Article Snippet: After 10 days, the youngest leaf of each plant was collected and DNA was isolated with the PowerPlant Pro DNA Isolation Kit (Mo Bio Laboratories, Carlsbad, CA, USA). .. The manufacturer’s instructions were followed and the yield and quality of the DNA was assessed via agarose gel electrophoresis and a Qubit 2.0 fluorimeter (ThermoFisher Scientific, Waltham, MA, USA).

    Article Title: Validation of an NGS mutation detection panel for melanoma
    Article Snippet: Paragraph title: DNA extraction ... DNA content and quality was checked by Qubit 2.0 fluorimeter (ThermoFisher) according to the manufacturer’s instructions using their HS dsDNA kit before mutation detection.

    Nucleic Acid Electrophoresis:

    Article Title: Extreme-Depth Re-sequencing of Mitochondrial DNA Finds No Evidence of Paternal Transmission in Humans
    Article Snippet: Initially, primer efficiency and specificity was assessed as successful after no amplification of DNA from rho0 cell lines, which contain no mtDNA. .. Amplified products were assessed by gel electrophoresis against DNA+ve and DNA-ve controls, and quantified using a Qubit 2.0 fluorimeter (Life Technologies, Paisley, UK). .. Each amplicon was individually purified using Agencourt AMPure XP beads (Beckman-Coulter, USA), pooled in equimolar concentrations and re-quantified.

    Article Title: Somatic mtDNA variation is an important component of Parkinson's disease
    Article Snippet: Initially, primer efficiency and specificity was assessed as successful after zero amplification of DNA from Rho0 cell lines, avoiding the unintended amplification of nuclear pseudogenes. .. Amplified products were assessed by gel electrophoresis, against DNA+ve and DNA−ve controls, and quantified using a Qubit 2.0 fluorimeter (Life Technologies, Paisley, UK). .. Each amplicon was individually purified using Agencourt AMPure XP beads (Beckman-Coulter, USA), pooled in equimolar concentrations and requantified.

    RNA Sequencing Assay:

    Article Title: Histone deacetylase class-I inhibition promotes epithelial gene expression in pancreatic cancer cells in a BRD4- and MYC-dependent manner
    Article Snippet: DNA was quantitated using the Qubit® dsDNA HS assay and a Qubit® 2.0 Fluorimeter (Invitrogen). .. Three to five nanogram of fragmented DNA was used for generating the ChIP-Seq libraries using the MicroPlex Library Preparation Kit (Diagenode) according to the manufacturer's instructions.

    Mutagenesis:

    Article Title: Validation of an NGS mutation detection panel for melanoma
    Article Snippet: DNA extraction was performed using the Promega Maxwell™ instrument, according to the manufacturer’s instructions. .. DNA content and quality was checked by Qubit 2.0 fluorimeter (ThermoFisher) according to the manufacturer’s instructions using their HS dsDNA kit before mutation detection. .. We used a volume of 1 μl (less than 50 ng DNA) to conserve as much of the sample as possible, and worked with a range of concentrations, depending on the extraction concentration achieved.

    Isolation:

    Article Title: Suppressor mutations in Rpf2–Rrs1 or Rpl5 bypass the Cgr1 function for pre-ribosomal 5S RNP-rotation
    Article Snippet: To estimate the integrity of the isolated genomic DNA, 2.5 µl of the preparation was migrated on a 1% agarose gel. .. The concentration of the genomic DNA was determined with a Qubit 2.0 fluorimeter (Invitrogen).

    Article Title: Transcriptome and secretome analysis of Aspergillus fumigatus in the presence of sugarcane bagasse
    Article Snippet: Paragraph title: RNA isolation and cDNA synthesis ... RNA was quantified on a Qubit® 2.0 fluorimeter (Thermo Fisher Scientific, Waltham, MS, EUA) with the Qubit® RNA BR Assay kit (Thermo Fisher Scientific, Waltham, MS, EUA). cDNA was synthesized from 1 μg of mRNA using SuperScript® II Reverse Transcriptase (Invitrogen, Carlsbad, CA, EUA).

    Article Title: Bleomycin-induced genome structural variations in normal, non-tumor cells
    Article Snippet: Total DNA was isolated from tissue and cultured cells using the Quick-gDNATM MiniPrep kit (Zymo Research Corporation, Irvine, CA, USA) following the manufacturer’s instructions. .. DNA concentration was determined using the Qubit 2.0 fluorimeter (Life Technologies Corporation, Carlsbad, CA, USA).

    Article Title: A Nosocomial Outbreak of Human Monkeypox in the Central African Republic
    Article Snippet: Paragraph title: Isolation of the Monkeypox Virus, DNA Extraction, and Molecular Assays ... DNA was quantified using the Qubit dsDNA BR Assay kit with the Qubit 2.0 fluorimeter (Life Technologies, CA).

    Article Title: Extreme-Depth Re-sequencing of Mitochondrial DNA Finds No Evidence of Paternal Transmission in Humans
    Article Snippet: Paragraph title: mtDNA isolation and enrichment ... Amplified products were assessed by gel electrophoresis against DNA+ve and DNA-ve controls, and quantified using a Qubit 2.0 fluorimeter (Life Technologies, Paisley, UK).

    Article Title: Somatic mtDNA variation is an important component of Parkinson's disease
    Article Snippet: Paragraph title: MtDNA isolation and enrichment ... Amplified products were assessed by gel electrophoresis, against DNA+ve and DNA−ve controls, and quantified using a Qubit 2.0 fluorimeter (Life Technologies, Paisley, UK).

    Article Title: Linking rhizosphere microbiome composition of wild and domesticated Phaseolus vulgaris to genotypic and root phenotypic traits
    Article Snippet: After 10 days, the youngest leaf of each plant was collected and DNA was isolated with the PowerPlant Pro DNA Isolation Kit (Mo Bio Laboratories, Carlsbad, CA, USA). .. The manufacturer’s instructions were followed and the yield and quality of the DNA was assessed via agarose gel electrophoresis and a Qubit 2.0 fluorimeter (ThermoFisher Scientific, Waltham, MA, USA).

    Purification:

    Article Title: Whole-genome sequencing reveals principles of brain retrotransposition in neurodevelopmental disorders
    Article Snippet: DNA was extracted from frozen brain tissues using Genomic DNA Lysis buffer (0.4 M NaCl, 1% SDS, 0.1 M Tris-HCl pH 8.1, 5 mM EDTA, 0.05% proteinase K) and purified with phenol:chloroform:isoamyl alcohol (PCI 25:24:1, Invitrogen, cat 15593-031) using PLG tubes (MaXtract, Qiagen, cat 129016). .. The yield and the quality of extracted DNA were assayed using a NanoDrop and a Qubit 2.0 Fluorimeter (Invitrogen, dsDNA HS Assay, cat ).

    Article Title: Transcriptome and secretome analysis of Aspergillus fumigatus in the presence of sugarcane bagasse
    Article Snippet: Total RNA was purified by using the “Direct-zol™ RNA MiniPrep” kit according to the manufacturer’s instructions (Zymo Research, Irvine, CA, EUA) using the on-column DNAse treatment. .. RNA was quantified on a Qubit® 2.0 fluorimeter (Thermo Fisher Scientific, Waltham, MS, EUA) with the Qubit® RNA BR Assay kit (Thermo Fisher Scientific, Waltham, MS, EUA). cDNA was synthesized from 1 μg of mRNA using SuperScript® II Reverse Transcriptase (Invitrogen, Carlsbad, CA, EUA).

    Article Title: A lanthipeptide library used to identify a protein–protein interaction inhibitor
    Article Snippet: The PCR products were purified by Qiagen PCR purification kit and subjected to the following Index PCR. .. DNA concentration was quantified with a Qubit® 2.0 fluorimeter (Invitrogen) using a Qubit HS dsDNA HS assay kit.

    Article Title: Putting the Mess in Order: Aspergillus welwitschiae (and Not A. niger) Is the Etiological Agent of Sisal Bole Rot Disease in Brazil
    Article Snippet: The integrity and quantification of genomic DNA were evaluated by fluorimetry using the Qubit 2.0 Fluorimeter® (Invitrogen). .. The integrity and quantification of genomic DNA were evaluated by fluorimetry using the Qubit 2.0 Fluorimeter® (Invitrogen).

    Article Title: Impact of demographics on human gut microbial diversity in a US Midwest population
    Article Snippet: PCR product sizes were verified using the Agilent TapeStation with reaction cleanup, and DNA was purified using an epMotion automated system (Eppendorf, Hauppauge, NY, USA) with the Agencourt AMPure PCR Purification System (Beckman Coulter, Brea, CA). .. Final quantitation was performed using a QuBit HS dsDNA kit and the QuBit 2.0 fluorimeter (Life Technologies, Carlsbad, CA, USA).

    Polymerase Chain Reaction:

    Article Title: A lanthipeptide library used to identify a protein–protein interaction inhibitor
    Article Snippet: The PCR products (appearing as a strong band at 200-250 bp) were gel-purified with Qiagen gel purification kit. .. DNA concentration was quantified with a Qubit® 2.0 fluorimeter (Invitrogen) using a Qubit HS dsDNA HS assay kit.

    Article Title: Putting the Mess in Order: Aspergillus welwitschiae (and Not A. niger) Is the Etiological Agent of Sisal Bole Rot Disease in Brazil
    Article Snippet: The integrity and quantification of genomic DNA were evaluated by fluorimetry using the Qubit 2.0 Fluorimeter® (Invitrogen). .. The integrity and quantification of genomic DNA were evaluated by fluorimetry using the Qubit 2.0 Fluorimeter® (Invitrogen).

    Article Title: Somatic mtDNA variation is an important component of Parkinson's disease
    Article Snippet: MtDNA was enriched using long-range polymerase chain reaction. .. Amplified products were assessed by gel electrophoresis, against DNA+ve and DNA−ve controls, and quantified using a Qubit 2.0 fluorimeter (Life Technologies, Paisley, UK).

    Article Title: Impact of demographics on human gut microbial diversity in a US Midwest population
    Article Snippet: PCR product sizes were verified using the Agilent TapeStation with reaction cleanup, and DNA was purified using an epMotion automated system (Eppendorf, Hauppauge, NY, USA) with the Agencourt AMPure PCR Purification System (Beckman Coulter, Brea, CA). .. Final quantitation was performed using a QuBit HS dsDNA kit and the QuBit 2.0 fluorimeter (Life Technologies, Carlsbad, CA, USA).

    Salting Out:

    Article Title: The human CIB1–EVER1–EVER2 complex governs keratinocyte-intrinsic immunity to β-papillomaviruses
    Article Snippet: For kindred F, DNA was extracted from peripheral blood samples by the salting out method. .. DNA concentration was measured with a Qubit 2.0 fluorimeter (Thermo Fisher Scientific).

    Agarose Gel Electrophoresis:

    Article Title: Suppressor mutations in Rpf2–Rrs1 or Rpl5 bypass the Cgr1 function for pre-ribosomal 5S RNP-rotation
    Article Snippet: To estimate the integrity of the isolated genomic DNA, 2.5 µl of the preparation was migrated on a 1% agarose gel. .. The concentration of the genomic DNA was determined with a Qubit 2.0 fluorimeter (Invitrogen).

    Article Title: Linking rhizosphere microbiome composition of wild and domesticated Phaseolus vulgaris to genotypic and root phenotypic traits
    Article Snippet: After 10 days, the youngest leaf of each plant was collected and DNA was isolated with the PowerPlant Pro DNA Isolation Kit (Mo Bio Laboratories, Carlsbad, CA, USA). .. The manufacturer’s instructions were followed and the yield and quality of the DNA was assessed via agarose gel electrophoresis and a Qubit 2.0 fluorimeter (ThermoFisher Scientific, Waltham, MA, USA). .. Genome profiling was performed using the complexity genome reduction method developed by Diversity Arrays Technology Pty Ltd (DArT P/L, Bruce, Australia) ( ).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Somatic mtDNA variation is an important component of Parkinson's disease
    Article Snippet: To eliminate the potential for error and nuclear DNA contamination, amplicons were polymerized using PrimeSTAR GXL DNA polymerase (error rate = 0.00108%, Takara Bio, Saint-Germain-en-Laye, France) in 2 overlapping fragments, using primer set-1: CCC TCT CTC CTA CTC CTG-F (m.6222–6239) and CAG GTG GTC AAG TAT TTA TGG–R (m.16133–16,153), and set-2: CAT CTT GCC CTT CAT TAT TGC–F (m.15295–15,315) and GGC AGG ATA GTT CAG ACG-R (7773–7791). .. Amplified products were assessed by gel electrophoresis, against DNA+ve and DNA−ve controls, and quantified using a Qubit 2.0 fluorimeter (Life Technologies, Paisley, UK).

    Mouse Assay:

    Article Title: A Nosocomial Outbreak of Human Monkeypox in the Central African Republic
    Article Snippet: The monkeypox virus was isolated and amplified by 1 passage of intracranial inoculation in the brains of newborn mice using pus or scab homogenates from patients, as previously described [ ]. .. DNA was quantified using the Qubit dsDNA BR Assay kit with the Qubit 2.0 fluorimeter (Life Technologies, CA).

    Chromatin Immunoprecipitation:

    Article Title: Histone deacetylase class-I inhibition promotes epithelial gene expression in pancreatic cancer cells in a BRD4- and MYC-dependent manner
    Article Snippet: Paragraph title: Chromatin immunoprecipitation, library preparation and next-generation sequencing ... DNA was quantitated using the Qubit® dsDNA HS assay and a Qubit® 2.0 Fluorimeter (Invitrogen).

    Plasmid Preparation:

    Article Title: A lanthipeptide library used to identify a protein–protein interaction inhibitor
    Article Snippet: Paragraph title: Sequencing of the plasmid library ... DNA concentration was quantified with a Qubit® 2.0 fluorimeter (Invitrogen) using a Qubit HS dsDNA HS assay kit.

    Software:

    Article Title: Putting the Mess in Order: Aspergillus welwitschiae (and Not A. niger) Is the Etiological Agent of Sisal Bole Rot Disease in Brazil
    Article Snippet: The integrity and quantification of genomic DNA were evaluated by fluorimetry using the Qubit 2.0 Fluorimeter® (Invitrogen). .. Successfully amplified PCR products were purified using the Illustra® GFX PCR DNA and Gel Band Purification kit (GE Healthcare Life Sciences) and sequenced on an ABI3130 automated sequencer (Applied Biosystems, Life Technologies Q7, CA, USA).

    Negative Control:

    Article Title: Histone deacetylase class-I inhibition promotes epithelial gene expression in pancreatic cancer cells in a BRD4- and MYC-dependent manner
    Article Snippet: Non-specific IgG was used as a negative control. .. DNA was quantitated using the Qubit® dsDNA HS assay and a Qubit® 2.0 Fluorimeter (Invitrogen).

    Sample Prep:

    Article Title: The human CIB1–EVER1–EVER2 complex governs keratinocyte-intrinsic immunity to β-papillomaviruses
    Article Snippet: An adapter-ligated library was prepared with the Paired-End Sample Prep kit V1 (Illumina). .. DNA concentration was measured with a Qubit 2.0 fluorimeter (Thermo Fisher Scientific).

    Article Title: Impact of demographics on human gut microbial diversity in a US Midwest population
    Article Snippet: Paragraph title: Sample preparation and sequencing ... Final quantitation was performed using a QuBit HS dsDNA kit and the QuBit 2.0 fluorimeter (Life Technologies, Carlsbad, CA, USA).

    Next-Generation Sequencing:

    Article Title: Suppressor mutations in Rpf2–Rrs1 or Rpl5 bypass the Cgr1 function for pre-ribosomal 5S RNP-rotation
    Article Snippet: The concentration of the genomic DNA was determined with a Qubit 2.0 fluorimeter (Invitrogen). .. Libraries were generated from 1 µg of genomic DNA and high-throughput sequencing was performed on a HiSeq 3000 instrument (Illumina).

    Article Title: Histone deacetylase class-I inhibition promotes epithelial gene expression in pancreatic cancer cells in a BRD4- and MYC-dependent manner
    Article Snippet: Paragraph title: Chromatin immunoprecipitation, library preparation and next-generation sequencing ... DNA was quantitated using the Qubit® dsDNA HS assay and a Qubit® 2.0 Fluorimeter (Invitrogen).

    Quantitation Assay:

    Article Title: Impact of demographics on human gut microbial diversity in a US Midwest population
    Article Snippet: PCR product sizes were verified using the Agilent TapeStation with reaction cleanup, and DNA was purified using an epMotion automated system (Eppendorf, Hauppauge, NY, USA) with the Agencourt AMPure PCR Purification System (Beckman Coulter, Brea, CA). .. Final quantitation was performed using a QuBit HS dsDNA kit and the QuBit 2.0 fluorimeter (Life Technologies, Carlsbad, CA, USA). .. Samples were pooled to equal concentration and sequenced on one lane of a MiSeq at the Mayo Genomics Facility using the MiSeq Reagent Kit v2 (2 × 250 reads, 500 cycles; Illumina Inc., San Diego, CA, USA).

    Spectrophotometry:

    Article Title: Prednisolone induces osteoporosis-like phenotypes via focal adhesion signaling pathway in zebrafish larvae
    Article Snippet: RNA purity was assessed using the NanoPhotome spectrophotometer (IMPLEN, West Lake Village, CA, USA). .. RNA concentration was measured using the Qubit® RNA Assay Kit in a Qubit® 2.0 Fluorimeter (Life Technologies).

    Immunoprecipitation:

    Article Title: Histone deacetylase class-I inhibition promotes epithelial gene expression in pancreatic cancer cells in a BRD4- and MYC-dependent manner
    Article Snippet: After sonication, precleared (50% Sepharose 4B from GE Healthcare) chromatin extract was subjected to immunoprecipitation by overnight incubation with the specific antibody. .. DNA was quantitated using the Qubit® dsDNA HS assay and a Qubit® 2.0 Fluorimeter (Invitrogen).

    DNA Purification:

    Article Title: Human papillomavirus detection using the Abbott RealTime high-risk HPV tests compared with conventional nested PCR coupled to high-throughput sequencing of amplification products in cervical smear specimens from a Gabonese female population
    Article Snippet: DNA was extracted from exfoliated cells obtained from liquid-based cytology (LBC) using the DNeasy Blood and Tissue kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions. .. DNA was quantified using the Qubit® dsDNA BR Assay kit with the Qubit 2.0 fluorimeter (Life Technologies, Carlsbad, CA, USA) primarily to confirm the previous DNA purification step. .. The AbRT assay (Abbott Molecular, Des Plaines, IL, USA) was performed with the m 2000rt automated analyzer according to the manufacturer’s instructions.

    CTG Assay:

    Article Title: Somatic mtDNA variation is an important component of Parkinson's disease
    Article Snippet: To eliminate the potential for error and nuclear DNA contamination, amplicons were polymerized using PrimeSTAR GXL DNA polymerase (error rate = 0.00108%, Takara Bio, Saint-Germain-en-Laye, France) in 2 overlapping fragments, using primer set-1: CCC TCT CTC CTA CTC CTG-F (m.6222–6239) and CAG GTG GTC AAG TAT TTA TGG–R (m.16133–16,153), and set-2: CAT CTT GCC CTT CAT TAT TGC–F (m.15295–15,315) and GGC AGG ATA GTT CAG ACG-R (7773–7791). .. Amplified products were assessed by gel electrophoresis, against DNA+ve and DNA−ve controls, and quantified using a Qubit 2.0 fluorimeter (Life Technologies, Paisley, UK).

    Lysis:

    Article Title: Whole-genome sequencing reveals principles of brain retrotransposition in neurodevelopmental disorders
    Article Snippet: DNA was extracted from frozen brain tissues using Genomic DNA Lysis buffer (0.4 M NaCl, 1% SDS, 0.1 M Tris-HCl pH 8.1, 5 mM EDTA, 0.05% proteinase K) and purified with phenol:chloroform:isoamyl alcohol (PCI 25:24:1, Invitrogen, cat 15593-031) using PLG tubes (MaXtract, Qiagen, cat 129016). .. The yield and the quality of extracted DNA were assayed using a NanoDrop and a Qubit 2.0 Fluorimeter (Invitrogen, dsDNA HS Assay, cat ).

    Article Title: Histone deacetylase class-I inhibition promotes epithelial gene expression in pancreatic cancer cells in a BRD4- and MYC-dependent manner
    Article Snippet: Nuclear pellets were resuspended in the lysis buffer and sonicated using a Bioruptor® Pico (Diagenode) with 30 s on/off pulse. .. DNA was quantitated using the Qubit® dsDNA HS assay and a Qubit® 2.0 Fluorimeter (Invitrogen).

    Article Title: Applied diagnostics in liver cancer. Efficient combinations of sorafenib with targeted inhibitors blocking AKT/mTOR
    Article Snippet: Briefly, PBS-washed samples, were centrifuged and lysed using “Tissue and cell lysis solution” buffer for the MasterPureTM kit, complemented by proteinase K (5 μl/100 μl buffer) (Epicenter), shaking overnight at 56°C. .. Genomic DNA was quantified using a Qubit ds DNA BR assay kit and a Qubit 2.0 fluorimeter (Invitrogen).

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    Thermo Fisher qubit 2 0 fluorometer
    Qubit 2 0 Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1177 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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