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R&D Systems quantikine elisa kit
Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/quantikine elisa kit/product/R&D Systems
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
quantikine elisa kit - by Bioz Stars, 2021-03
86/100 stars

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Enzyme-linked Immunosorbent Assay:

Article Title: Interleukin-17A is involved in mechanical hyperalgesia but not in the severity of murine antigen-induced arthritis
Article Snippet: Cytokines from cell culture, except IL-17F, were measured by Luminex Multiplex Detection technology using the Milliplex MTH17MAG-47K-09 kit (Millipore, Schwalbach, Germany) according to the manufacturer’s instructions. .. IL-17F was measured with a Quantikine® ELISA kit (R & D Systems, Wiesbaden, Germany). .. Cytokines in serum were measured using standard sandwich ELISA procedures .

Article Title: Bone marrow-derived mesenchymal stem cells effectively regenerate fibrotic liver in bile duct ligation rat model
Article Snippet: Lipid peroxides, expressed as malondialdehyde (MDA), and GSH were determined in the resultant 10% homogenate using Biodiagnostics kits (Biodiagnostics Co., Giza, Egypt). .. HGF was measured in liver tissue after cell lysis using Quantikine® ELISA kit (R & D Systems Inc, USA). .. Total RNA was extracted from liver samples using SV Total RNA isolation system (Promega, Madison, WI), reverse transcribed into cDNA and amplified by PCR using RT-PCR kit (Stratagene, USA).

Article Title: The diagnostic and prognostic value of interleukin-6 in patients with soft tissue sarcomas
Article Snippet: .. Samples underwent centrifugation at 1,000 × g for 15 min and were measured using the R & D Systems™ Quantikine® ELISA kit (Minneapolis, USA). ..

Article Title: Tuberculous lymphadenitis is associated with altered levels of circulating angiogenic factors
Article Snippet: .. VEGF-D and TIE2 levels were measured using a Quantikine® ELISA kit (R & D Systems). ..

Article Title: Simultaneous changes in high-fat and high-cholesterol diet-induced steatohepatitis and severe fibrosis and those underlying molecular mechanisms in novel SHRSP5/Dmcr rat
Article Snippet: Plasma total adiponectin concentration was determined by an enzyme-linked immunosorbent assay (ELISA) kit (Otsuka Pharmaceutical, Tokyo, Japan). .. Similarly, plasma TNF-α concentrations were quantitatively measured by the Quantikine® ELISA kit (R & D Systems, Minneapolis, MN). ..

Article Title: Serum Interleukin-18, Fetuin-A, Soluble Intercellular Adhesion Molecule-1, and Endothelin-1 in Ankylosing Spondylitis, Psoriatic Arthritis, and SAPHO Syndrome
Article Snippet: Disease activity score calculation in patients with peripheral arthritis was performed using a free online Disease Activity Score 28 (DAS28) calculator. .. Serum was stored at −80 °C until analysis for IL-18, fetuin-A, sICAM-1, ET-1, IL-6, IL-23, VEGF, and EGF using a sensitive sandwich ELISA method: Human IL-18 Quantitative ELISA kit (MBL, Nagoya, Japan), Human Fetuin-A Immunoassay Quantikine® ELISA kit, Human sICAM-1 Immunoassay Quantikine® ELISA kit, Human ET-1 Immunoassay Quantikine® ELISA kit, Human IL-6 Immunoassay Quantikine® ELISA kit, Human IL-23 Immunoassay Quantikine® ELISA kit, Human VEGF Immunoassay Quantikine® ELISA kit, and Human EGF Immunoassay Quantikine® ELISA kit (R & D Systems, Minneapolis, MN, USA). .. All analyses and calibrations were performed in duplicate and read using a BioTek PowerWaveXS spectrophotometer (Winooski, VT, USA).

Article Title: Effect of a combined estrogen and progesterone oral contraceptive on circulating adipocytokines adiponectin, resistin and DLK-1 in normal and obese female rhesus monkeys
Article Snippet: Adiponectin levels were measured using rhesus macaque Adiponectin Competitive ELISA kit from Adipo-Gen, Inc (Seoul, Korea). .. DLK-1 levels were measured using Quantikine® ELISA kit from R & D Systems (Minneapolis, MN, USA). .. Resistin levels were measured using Human Resistin ELISA kits from Biovendor Research and Diagnostic Products (Czech Republic).

Lysis:

Article Title: Bone marrow-derived mesenchymal stem cells effectively regenerate fibrotic liver in bile duct ligation rat model
Article Snippet: Lipid peroxides, expressed as malondialdehyde (MDA), and GSH were determined in the resultant 10% homogenate using Biodiagnostics kits (Biodiagnostics Co., Giza, Egypt). .. HGF was measured in liver tissue after cell lysis using Quantikine® ELISA kit (R & D Systems Inc, USA). .. Total RNA was extracted from liver samples using SV Total RNA isolation system (Promega, Madison, WI), reverse transcribed into cDNA and amplified by PCR using RT-PCR kit (Stratagene, USA).

Centrifugation:

Article Title: The diagnostic and prognostic value of interleukin-6 in patients with soft tissue sarcomas
Article Snippet: .. Samples underwent centrifugation at 1,000 × g for 15 min and were measured using the R & D Systems™ Quantikine® ELISA kit (Minneapolis, USA). ..

Sandwich ELISA:

Article Title: Serum Interleukin-18, Fetuin-A, Soluble Intercellular Adhesion Molecule-1, and Endothelin-1 in Ankylosing Spondylitis, Psoriatic Arthritis, and SAPHO Syndrome
Article Snippet: Disease activity score calculation in patients with peripheral arthritis was performed using a free online Disease Activity Score 28 (DAS28) calculator. .. Serum was stored at −80 °C until analysis for IL-18, fetuin-A, sICAM-1, ET-1, IL-6, IL-23, VEGF, and EGF using a sensitive sandwich ELISA method: Human IL-18 Quantitative ELISA kit (MBL, Nagoya, Japan), Human Fetuin-A Immunoassay Quantikine® ELISA kit, Human sICAM-1 Immunoassay Quantikine® ELISA kit, Human ET-1 Immunoassay Quantikine® ELISA kit, Human IL-6 Immunoassay Quantikine® ELISA kit, Human IL-23 Immunoassay Quantikine® ELISA kit, Human VEGF Immunoassay Quantikine® ELISA kit, and Human EGF Immunoassay Quantikine® ELISA kit (R & D Systems, Minneapolis, MN, USA). .. All analyses and calibrations were performed in duplicate and read using a BioTek PowerWaveXS spectrophotometer (Winooski, VT, USA).

Article Title: BMP9 Protects Septal Neurons from Axotomy-Evoked Loss of Cholinergic Phenotype
Article Snippet: .. The Quantikine® sandwich ELISA kit (R & D Systems) was used to assay FGF-2 levels in the samples. ..

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  • 99
    R&D Systems sandwich style enzyme linked immunosorbent assays
    Galantamine does not reduce markers of inflammation in brain-injured rats. ( A ) ELISA measurements of IL-1ß in serum samples obtained from sham, injured animals receiving vehicle, and injured animals receiving 1.0 mg/kg of galantamine at 24 h and 6 days post-injury ( n = 4/group/time point). The modest increase in plasma IL-1ß (observed 24 h post-injury) was not affected by galantamine treatment. ( B ) The increases in plasma HMGB1 (measured using western blots) in TBI animals were unaffected by post-injury galantamine. ( C ) Tissue IL-1ß was markedly increased in the injured cortical tissue compared to sham (undetectable by ELISA). This increase was not influenced by galantamine. ( D–F ) Quantification of mRNA for markers of the M1 microglial/macrophage phenotype: nitric oxide synthase 2 (Nos2; D), Toll-like receptor 4 (TLR4; E), and interleukin-12ß (IL-12ß; F) indicate increases in M1 activation after TBI. However, galantamine did not change the levels of these markers. ( G–I ) Quantification of mRNA markers of the M2 microglial/macrophage phenotype: arginase1 (Arg1; G), chemokine (C-C motif) ligand 17 (CCL17; H), and mannose receptor C-type 1 (Mrc1; I) after TBI. The mRNA levels of these markers were unaffected by galantamine. CCI, controlled cortical impact; ELISA, enzyme-linked <t>immunosorbent</t> assay; Gal, galantamine; HMGB1, high-mobility group box 1; mRNA, messenger RNA; TBI, traumatic brain injury; Veh, vehicle.
    Sandwich Style Enzyme Linked Immunosorbent Assays, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sandwich style enzyme linked immunosorbent assays/product/R&D Systems
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sandwich style enzyme linked immunosorbent assays - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    86
    R&D Systems mouse rat il 33 quantikine elisa kit
    CLP302-253 blocks activation of primary lymphoid cells in vitro . (A and B) B cells were activated with ODN2006 and αIgM F(ab’) 2 for 72 h. To some cultures, CPL302-253 was added. (A) Expression of and activation marker CD25 and (B) proliferation marker ki-67 was assessed by FACS. (C) CD8 T cells were activated with αCD2, αCD3 and αCD28. IFNγ release was measured after 48 h in culture supernatants by ELISA. (D) A549 cells were co-cultured with naïve CD8 or activated CD8 cells. To some cultures CPL302-253 was added at 1 μM. <t>IL-33</t> expression was assessed after 72 h of treatment in CD8 and A549 cell lysates by ELISA. For each experiment at least three independent biological replicates were performed. p-value
    Mouse Rat Il 33 Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse rat il 33 quantikine elisa kit/product/R&D Systems
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse rat il 33 quantikine elisa kit - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    R&D Systems mouse rat fgf basic quantikine elisa kit
    Quantification of <t>FGF</t> immobilized on polydopamine-coated PXDDA discs using <t>ELISA.</t>
    Mouse Rat Fgf Basic Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse rat fgf basic quantikine elisa kit/product/R&D Systems
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse rat fgf basic quantikine elisa kit - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    99
    R&D Systems elisa kit
    Long-term <t>CNTF</t> secretion from encapsulated C2C12 cells. A , Long-term survival of alginate encapsulated CNTF-expressing C2C12 cells as shown by eGFP expression at 1 week and 8 months in culture. B , Immunoblot of conditioned medium from CNTF producing C2C12 capsules and from the corresponding cell monolayer. C , Mean ± SEM hCNTF secretion per 24 h from one alginate bead determined by <t>ELISA</t> at different time points in culture ( n = 3–9 per time point).
    Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa kit/product/R&D Systems
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    elisa kit - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

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    Galantamine does not reduce markers of inflammation in brain-injured rats. ( A ) ELISA measurements of IL-1ß in serum samples obtained from sham, injured animals receiving vehicle, and injured animals receiving 1.0 mg/kg of galantamine at 24 h and 6 days post-injury ( n = 4/group/time point). The modest increase in plasma IL-1ß (observed 24 h post-injury) was not affected by galantamine treatment. ( B ) The increases in plasma HMGB1 (measured using western blots) in TBI animals were unaffected by post-injury galantamine. ( C ) Tissue IL-1ß was markedly increased in the injured cortical tissue compared to sham (undetectable by ELISA). This increase was not influenced by galantamine. ( D–F ) Quantification of mRNA for markers of the M1 microglial/macrophage phenotype: nitric oxide synthase 2 (Nos2; D), Toll-like receptor 4 (TLR4; E), and interleukin-12ß (IL-12ß; F) indicate increases in M1 activation after TBI. However, galantamine did not change the levels of these markers. ( G–I ) Quantification of mRNA markers of the M2 microglial/macrophage phenotype: arginase1 (Arg1; G), chemokine (C-C motif) ligand 17 (CCL17; H), and mannose receptor C-type 1 (Mrc1; I) after TBI. The mRNA levels of these markers were unaffected by galantamine. CCI, controlled cortical impact; ELISA, enzyme-linked immunosorbent assay; Gal, galantamine; HMGB1, high-mobility group box 1; mRNA, messenger RNA; TBI, traumatic brain injury; Veh, vehicle.

    Journal: Journal of Neurotrauma

    Article Title: Post-Injury Administration of Galantamine Reduces Traumatic Brain Injury Pathology and Improves Outcome

    doi: 10.1089/neu.2017.5102

    Figure Lengend Snippet: Galantamine does not reduce markers of inflammation in brain-injured rats. ( A ) ELISA measurements of IL-1ß in serum samples obtained from sham, injured animals receiving vehicle, and injured animals receiving 1.0 mg/kg of galantamine at 24 h and 6 days post-injury ( n = 4/group/time point). The modest increase in plasma IL-1ß (observed 24 h post-injury) was not affected by galantamine treatment. ( B ) The increases in plasma HMGB1 (measured using western blots) in TBI animals were unaffected by post-injury galantamine. ( C ) Tissue IL-1ß was markedly increased in the injured cortical tissue compared to sham (undetectable by ELISA). This increase was not influenced by galantamine. ( D–F ) Quantification of mRNA for markers of the M1 microglial/macrophage phenotype: nitric oxide synthase 2 (Nos2; D), Toll-like receptor 4 (TLR4; E), and interleukin-12ß (IL-12ß; F) indicate increases in M1 activation after TBI. However, galantamine did not change the levels of these markers. ( G–I ) Quantification of mRNA markers of the M2 microglial/macrophage phenotype: arginase1 (Arg1; G), chemokine (C-C motif) ligand 17 (CCL17; H), and mannose receptor C-type 1 (Mrc1; I) after TBI. The mRNA levels of these markers were unaffected by galantamine. CCI, controlled cortical impact; ELISA, enzyme-linked immunosorbent assay; Gal, galantamine; HMGB1, high-mobility group box 1; mRNA, messenger RNA; TBI, traumatic brain injury; Veh, vehicle.

    Article Snippet: Rat plasma and tissue interleukin (IL)-1ß levels were assessed using sandwich-style enzyme-linked immunosorbent assays (ELISAs; Quantikine IL-1ß assay; R & D Systems, Minneapolis, MN).

    Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Activation Assay

    CLP302-253 blocks activation of primary lymphoid cells in vitro . (A and B) B cells were activated with ODN2006 and αIgM F(ab’) 2 for 72 h. To some cultures, CPL302-253 was added. (A) Expression of and activation marker CD25 and (B) proliferation marker ki-67 was assessed by FACS. (C) CD8 T cells were activated with αCD2, αCD3 and αCD28. IFNγ release was measured after 48 h in culture supernatants by ELISA. (D) A549 cells were co-cultured with naïve CD8 or activated CD8 cells. To some cultures CPL302-253 was added at 1 μM. IL-33 expression was assessed after 72 h of treatment in CD8 and A549 cell lysates by ELISA. For each experiment at least three independent biological replicates were performed. p-value

    Journal: PLoS ONE

    Article Title: Preclinical characterization of CPL302-253, a selective inhibitor of PI3Kδ, as the candidate for the inhalatory treatment and prevention of Asthma

    doi: 10.1371/journal.pone.0236159

    Figure Lengend Snippet: CLP302-253 blocks activation of primary lymphoid cells in vitro . (A and B) B cells were activated with ODN2006 and αIgM F(ab’) 2 for 72 h. To some cultures, CPL302-253 was added. (A) Expression of and activation marker CD25 and (B) proliferation marker ki-67 was assessed by FACS. (C) CD8 T cells were activated with αCD2, αCD3 and αCD28. IFNγ release was measured after 48 h in culture supernatants by ELISA. (D) A549 cells were co-cultured with naïve CD8 or activated CD8 cells. To some cultures CPL302-253 was added at 1 μM. IL-33 expression was assessed after 72 h of treatment in CD8 and A549 cell lysates by ELISA. For each experiment at least three independent biological replicates were performed. p-value

    Article Snippet: IL-33 levels were measured in BALF using Mouse/Rat IL-33 Quantikine ELISA Kit (R & D Systems) from the supernatant of the BAL fluid.

    Techniques: Activation Assay, In Vitro, Expressing, Marker, FACS, Enzyme-linked Immunosorbent Assay, Cell Culture

    CPL302-253 is bocks eosinophil infiltration to the lungs and IL-33 expression in HDM induced asthma mouse model. Balb/c mice were immunized by s.c. route with CFA mixed with HDM lyophilized extract or saline. On day 12, 13 and 14 the mice were administrated by i.n. route with CPL302-253 or saline. On day 14, 60 min after compound administration the animals were challenged by i.n. route with HDM in sterile saline or saline. On day 15 eosinophils number (A) and IL-33 concentration (B) were measured in BAL.

    Journal: PLoS ONE

    Article Title: Preclinical characterization of CPL302-253, a selective inhibitor of PI3Kδ, as the candidate for the inhalatory treatment and prevention of Asthma

    doi: 10.1371/journal.pone.0236159

    Figure Lengend Snippet: CPL302-253 is bocks eosinophil infiltration to the lungs and IL-33 expression in HDM induced asthma mouse model. Balb/c mice were immunized by s.c. route with CFA mixed with HDM lyophilized extract or saline. On day 12, 13 and 14 the mice were administrated by i.n. route with CPL302-253 or saline. On day 14, 60 min after compound administration the animals were challenged by i.n. route with HDM in sterile saline or saline. On day 15 eosinophils number (A) and IL-33 concentration (B) were measured in BAL.

    Article Snippet: IL-33 levels were measured in BALF using Mouse/Rat IL-33 Quantikine ELISA Kit (R & D Systems) from the supernatant of the BAL fluid.

    Techniques: Expressing, Mouse Assay, Concentration Assay

    Quantification of FGF immobilized on polydopamine-coated PXDDA discs using ELISA.

    Journal: Scientific Reports

    Article Title: Immobilization of FGF on Poly(xylitol dodecanedioic Acid) Polymer for Tissue Regeneration

    doi: 10.1038/s41598-020-67261-6

    Figure Lengend Snippet: Quantification of FGF immobilized on polydopamine-coated PXDDA discs using ELISA.

    Article Snippet: Recombinant Mouse FGF basic and Mouse/Rat FGF basic Quantikine ELISA Kit were purchased from R & D Systems.

    Techniques: Enzyme-linked Immunosorbent Assay

    Long-term CNTF secretion from encapsulated C2C12 cells. A , Long-term survival of alginate encapsulated CNTF-expressing C2C12 cells as shown by eGFP expression at 1 week and 8 months in culture. B , Immunoblot of conditioned medium from CNTF producing C2C12 capsules and from the corresponding cell monolayer. C , Mean ± SEM hCNTF secretion per 24 h from one alginate bead determined by ELISA at different time points in culture ( n = 3–9 per time point).

    Journal: The Journal of Neuroscience

    Article Title: Ciliary Neurotrophic Factor Cell-Based Delivery Prevents Synaptic Impairment and Improves Memory in Mouse Models of Alzheimer's Disease

    doi: 10.1523/JNEUROSCI.4182-09.2010

    Figure Lengend Snippet: Long-term CNTF secretion from encapsulated C2C12 cells. A , Long-term survival of alginate encapsulated CNTF-expressing C2C12 cells as shown by eGFP expression at 1 week and 8 months in culture. B , Immunoblot of conditioned medium from CNTF producing C2C12 capsules and from the corresponding cell monolayer. C , Mean ± SEM hCNTF secretion per 24 h from one alginate bead determined by ELISA at different time points in culture ( n = 3–9 per time point).

    Article Snippet: To determine the concentration of hCNTF secreted from beads, triplicates of 10 beads were incubated in 500 μl of medium (DMEM, 10% FCS, 1× PS, 2 m m Gln, and 1× NEAA) for 48 h. The medium was harvested, and CNTF was measured using an ELISA kit for human CNTF (Quantikine, Human CNTF; R & D Systems Europe).

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay

    CNTF delivery prevents synaptic impairment and preserves cell signaling independently of amyloid in Tg2576 AD mouse model. After the behavioral analysis was completed, mice were killed, and synaptosomes from different brain regions were prepared as described in Materials and Methods. A , Representative immunoblots of proteins from hippocampal synaptosomes of Tg CTRL or Tg CNTF mice (2 synaptosomal preparations per condition are shown). B , Densitometric analysis was performed by normalization to actin. Data are presented as means ± SEM of three separate immunoblots ( n = 6 mice per group). C , Representative immunoblots of proteins from cortical synaptosomes of Tg CTRL or Tg CNTF mice (2 synaptosomal preparations per condition are shown). D , Correlation between GluR1 level and long-term memory in Tg CTRL or Tg CNTF mice. E , Representative immunoblots of CNTF-related signaling proteins in hippocampal synaptosomes of Tg CTRL or Tg CNTF mice (2 synaptosomal preparations per condition are shown). F , Brain levels of Aβ 40 and Aβ 42 peptides (fibrillar and soluble) were measured by ELISA tests in 9-month-old Tg CTRL or Tg CNTF mice ( n = 6 mice per group). Data are presented as the mean ± SEM and were not significantly different between Tg CTRL and Tg CNTF mice. # p

    Journal: The Journal of Neuroscience

    Article Title: Ciliary Neurotrophic Factor Cell-Based Delivery Prevents Synaptic Impairment and Improves Memory in Mouse Models of Alzheimer's Disease

    doi: 10.1523/JNEUROSCI.4182-09.2010

    Figure Lengend Snippet: CNTF delivery prevents synaptic impairment and preserves cell signaling independently of amyloid in Tg2576 AD mouse model. After the behavioral analysis was completed, mice were killed, and synaptosomes from different brain regions were prepared as described in Materials and Methods. A , Representative immunoblots of proteins from hippocampal synaptosomes of Tg CTRL or Tg CNTF mice (2 synaptosomal preparations per condition are shown). B , Densitometric analysis was performed by normalization to actin. Data are presented as means ± SEM of three separate immunoblots ( n = 6 mice per group). C , Representative immunoblots of proteins from cortical synaptosomes of Tg CTRL or Tg CNTF mice (2 synaptosomal preparations per condition are shown). D , Correlation between GluR1 level and long-term memory in Tg CTRL or Tg CNTF mice. E , Representative immunoblots of CNTF-related signaling proteins in hippocampal synaptosomes of Tg CTRL or Tg CNTF mice (2 synaptosomal preparations per condition are shown). F , Brain levels of Aβ 40 and Aβ 42 peptides (fibrillar and soluble) were measured by ELISA tests in 9-month-old Tg CTRL or Tg CNTF mice ( n = 6 mice per group). Data are presented as the mean ± SEM and were not significantly different between Tg CTRL and Tg CNTF mice. # p

    Article Snippet: To determine the concentration of hCNTF secreted from beads, triplicates of 10 beads were incubated in 500 μl of medium (DMEM, 10% FCS, 1× PS, 2 m m Gln, and 1× NEAA) for 48 h. The medium was harvested, and CNTF was measured using an ELISA kit for human CNTF (Quantikine, Human CNTF; R & D Systems Europe).

    Techniques: Mouse Assay, Western Blot, Enzyme-linked Immunosorbent Assay