quantikine elisa kit  (R&D Systems)

Journal: Journal of Neurotrauma

Article Title: Post-Injury Administration of Galantamine Reduces Traumatic Brain Injury Pathology and Improves Outcome

doi: 10.1089/neu.2017.5102

Figure Lengend Snippet: Galantamine does not reduce markers of inflammation in brain-injured rats. ( A ) ELISA measurements of IL-1ß in serum samples obtained from sham, injured animals receiving vehicle, and injured animals receiving 1.0 mg/kg of galantamine at 24 h and 6 days post-injury ( n = 4/group/time point). The modest increase in plasma IL-1ß (observed 24 h post-injury) was not affected by galantamine treatment. ( B ) The increases in plasma HMGB1 (measured using western blots) in TBI animals were unaffected by post-injury galantamine. ( C ) Tissue IL-1ß was markedly increased in the injured cortical tissue compared to sham (undetectable by ELISA). This increase was not influenced by galantamine. ( D–F ) Quantification of mRNA for markers of the M1 microglial/macrophage phenotype: nitric oxide synthase 2 (Nos2; D), Toll-like receptor 4 (TLR4; E), and interleukin-12ß (IL-12ß; F) indicate increases in M1 activation after TBI. However, galantamine did not change the levels of these markers. ( G–I ) Quantification of mRNA markers of the M2 microglial/macrophage phenotype: arginase1 (Arg1; G), chemokine (C-C motif) ligand 17 (CCL17; H), and mannose receptor C-type 1 (Mrc1; I) after TBI. The mRNA levels of these markers were unaffected by galantamine. CCI, controlled cortical impact; ELISA, enzyme-linked immunosorbent assay; Gal, galantamine; HMGB1, high-mobility group box 1; mRNA, messenger RNA; TBI, traumatic brain injury; Veh, vehicle.

Article Snippet: Rat plasma and tissue interleukin (IL)-1ß levels were assessed using sandwich-style enzyme-linked immunosorbent assays (ELISAs; Quantikine IL-1ß assay; R & D Systems, Minneapolis, MN).

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Activation Assay

Journal: PLoS ONE

Article Title: Preclinical characterization of CPL302-253, a selective inhibitor of PI3Kδ, as the candidate for the inhalatory treatment and prevention of Asthma

doi: 10.1371/journal.pone.0236159

Figure Lengend Snippet: CLP302-253 blocks activation of primary lymphoid cells in vitro . (A and B) B cells were activated with ODN2006 and αIgM F(ab’) 2 for 72 h. To some cultures, CPL302-253 was added. (A) Expression of and activation marker CD25 and (B) proliferation marker ki-67 was assessed by FACS. (C) CD8 T cells were activated with αCD2, αCD3 and αCD28. IFNγ release was measured after 48 h in culture supernatants by ELISA. (D) A549 cells were co-cultured with naïve CD8 or activated CD8 cells. To some cultures CPL302-253 was added at 1 μM. IL-33 expression was assessed after 72 h of treatment in CD8 and A549 cell lysates by ELISA. For each experiment at least three independent biological replicates were performed. p-value

Article Snippet: IL-33 levels were measured in BALF using Mouse/Rat IL-33 Quantikine ELISA Kit (R & D Systems) from the supernatant of the BAL fluid.

Techniques: Activation Assay, In Vitro, Expressing, Marker, FACS, Enzyme-linked Immunosorbent Assay, Cell Culture

Journal: PLoS ONE

Article Title: Preclinical characterization of CPL302-253, a selective inhibitor of PI3Kδ, as the candidate for the inhalatory treatment and prevention of Asthma

doi: 10.1371/journal.pone.0236159

Figure Lengend Snippet: CPL302-253 is bocks eosinophil infiltration to the lungs and IL-33 expression in HDM induced asthma mouse model. Balb/c mice were immunized by s.c. route with CFA mixed with HDM lyophilized extract or saline. On day 12, 13 and 14 the mice were administrated by i.n. route with CPL302-253 or saline. On day 14, 60 min after compound administration the animals were challenged by i.n. route with HDM in sterile saline or saline. On day 15 eosinophils number (A) and IL-33 concentration (B) were measured in BAL.

Article Snippet: IL-33 levels were measured in BALF using Mouse/Rat IL-33 Quantikine ELISA Kit (R & D Systems) from the supernatant of the BAL fluid.

Techniques: Expressing, Mouse Assay, Concentration Assay

Journal: Scientific Reports

Article Title: Immobilization of FGF on Poly(xylitol dodecanedioic Acid) Polymer for Tissue Regeneration

doi: 10.1038/s41598-020-67261-6

Figure Lengend Snippet: Quantification of FGF immobilized on polydopamine-coated PXDDA discs using ELISA.

Article Snippet: Recombinant Mouse FGF basic and Mouse/Rat FGF basic Quantikine ELISA Kit were purchased from R & D Systems.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: The Journal of Neuroscience

Article Title: Ciliary Neurotrophic Factor Cell-Based Delivery Prevents Synaptic Impairment and Improves Memory in Mouse Models of Alzheimer's Disease

doi: 10.1523/JNEUROSCI.4182-09.2010

Figure Lengend Snippet: Long-term CNTF secretion from encapsulated C2C12 cells. A , Long-term survival of alginate encapsulated CNTF-expressing C2C12 cells as shown by eGFP expression at 1 week and 8 months in culture. B , Immunoblot of conditioned medium from CNTF producing C2C12 capsules and from the corresponding cell monolayer. C , Mean ± SEM hCNTF secretion per 24 h from one alginate bead determined by ELISA at different time points in culture ( n = 3–9 per time point).

Article Snippet: To determine the concentration of hCNTF secreted from beads, triplicates of 10 beads were incubated in 500 μl of medium (DMEM, 10% FCS, 1× PS, 2 m m Gln, and 1× NEAA) for 48 h. The medium was harvested, and CNTF was measured using an ELISA kit for human CNTF (Quantikine, Human CNTF; R & D Systems Europe).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: The Journal of Neuroscience

Article Title: Ciliary Neurotrophic Factor Cell-Based Delivery Prevents Synaptic Impairment and Improves Memory in Mouse Models of Alzheimer's Disease

doi: 10.1523/JNEUROSCI.4182-09.2010

Figure Lengend Snippet: CNTF delivery prevents synaptic impairment and preserves cell signaling independently of amyloid in Tg2576 AD mouse model. After the behavioral analysis was completed, mice were killed, and synaptosomes from different brain regions were prepared as described in Materials and Methods. A , Representative immunoblots of proteins from hippocampal synaptosomes of Tg CTRL or Tg CNTF mice (2 synaptosomal preparations per condition are shown). B , Densitometric analysis was performed by normalization to actin. Data are presented as means ± SEM of three separate immunoblots ( n = 6 mice per group). C , Representative immunoblots of proteins from cortical synaptosomes of Tg CTRL or Tg CNTF mice (2 synaptosomal preparations per condition are shown). D , Correlation between GluR1 level and long-term memory in Tg CTRL or Tg CNTF mice. E , Representative immunoblots of CNTF-related signaling proteins in hippocampal synaptosomes of Tg CTRL or Tg CNTF mice (2 synaptosomal preparations per condition are shown). F , Brain levels of Aβ 40 and Aβ 42 peptides (fibrillar and soluble) were measured by ELISA tests in 9-month-old Tg CTRL or Tg CNTF mice ( n = 6 mice per group). Data are presented as the mean ± SEM and were not significantly different between Tg CTRL and Tg CNTF mice. # p

Article Snippet: To determine the concentration of hCNTF secreted from beads, triplicates of 10 beads were incubated in 500 μl of medium (DMEM, 10% FCS, 1× PS, 2 m m Gln, and 1× NEAA) for 48 h. The medium was harvested, and CNTF was measured using an ELISA kit for human CNTF (Quantikine, Human CNTF; R & D Systems Europe).

Techniques: Mouse Assay, Western Blot, Enzyme-linked Immunosorbent Assay