quantigene singleplex mirna probes  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    siRNA and miRNA Transfection Reagents
    Description:

    Catalog Number:
    mirna
    Price:
    None
    Buy from Supplier


    Structured Review

    Thermo Fisher quantigene singleplex mirna probes
    NF2 is downregulated by miR-92a-3p via its 3′UTR. (A) Schematic representation of the full and cloned 3′UTR region of NF2 isoform I mRNA and the predicted miR-92a-3p MREs within its sequence. Dual-Luciferase assay of (B) HCT116 and (C) A549 cells transfected with empty vector (i.e., pmirGLO) or NF2 −3′UTR-wild-type. (D and E) The expression levels of mature miR-92a-3p were measured by <t>QuantiGene</t> <t>miRNA</t> assay in untransfected (UTC) or miR-92a construct-transfected (OE) HCT116 and A549 cells. Dual-Luciferase assay of (F) HCT116 and (G) A549 cells co-transfected with increasing plasmid ratios of NF2 −3′UTR-wild-type:miR-92a. (H) Secondary structure analysis of the RNA-RNA interaction (boxed region) between miR-92a-3p (red) and its MRE within wild-type vs. mutant NF2 −3′UTR (green). Dual-Luciferase assay of (I) HCT116 and (J) A549 cells co-transfected with wild-type or miR-92a-3p MRE mutant NF2 −3′UTR and miR-92a expression vectors. All experiments were performed in cells maintained in 0.5% serum. Data presented are representative of three independent trials and expressed as the mean ± standard deviation (SD). *P≤0.05, **P≤0.01, ***P≤0.001. NF2 , neurofibromin 2; UTR, untranslated region; MRE, microRNA response element.

    https://www.bioz.com/result/quantigene singleplex mirna probes/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quantigene singleplex mirna probes - by Bioz Stars, 2020-04
    99/100 stars

    Related Products / Commonly Used Together

    pre-designed probes

    Images

    1) Product Images from "MicroRNA-92a promotes cell proliferation, migration and survival by directly targeting the tumor suppressor gene NF2 in colorectal and lung cancer cells"

    Article Title: MicroRNA-92a promotes cell proliferation, migration and survival by directly targeting the tumor suppressor gene NF2 in colorectal and lung cancer cells

    Journal: Oncology Reports

    doi: 10.3892/or.2019.7020

    NF2 is downregulated by miR-92a-3p via its 3′UTR. (A) Schematic representation of the full and cloned 3′UTR region of NF2 isoform I mRNA and the predicted miR-92a-3p MREs within its sequence. Dual-Luciferase assay of (B) HCT116 and (C) A549 cells transfected with empty vector (i.e., pmirGLO) or NF2 −3′UTR-wild-type. (D and E) The expression levels of mature miR-92a-3p were measured by QuantiGene miRNA assay in untransfected (UTC) or miR-92a construct-transfected (OE) HCT116 and A549 cells. Dual-Luciferase assay of (F) HCT116 and (G) A549 cells co-transfected with increasing plasmid ratios of NF2 −3′UTR-wild-type:miR-92a. (H) Secondary structure analysis of the RNA-RNA interaction (boxed region) between miR-92a-3p (red) and its MRE within wild-type vs. mutant NF2 −3′UTR (green). Dual-Luciferase assay of (I) HCT116 and (J) A549 cells co-transfected with wild-type or miR-92a-3p MRE mutant NF2 −3′UTR and miR-92a expression vectors. All experiments were performed in cells maintained in 0.5% serum. Data presented are representative of three independent trials and expressed as the mean ± standard deviation (SD). *P≤0.05, **P≤0.01, ***P≤0.001. NF2 , neurofibromin 2; UTR, untranslated region; MRE, microRNA response element.
    Figure Legend Snippet: NF2 is downregulated by miR-92a-3p via its 3′UTR. (A) Schematic representation of the full and cloned 3′UTR region of NF2 isoform I mRNA and the predicted miR-92a-3p MREs within its sequence. Dual-Luciferase assay of (B) HCT116 and (C) A549 cells transfected with empty vector (i.e., pmirGLO) or NF2 −3′UTR-wild-type. (D and E) The expression levels of mature miR-92a-3p were measured by QuantiGene miRNA assay in untransfected (UTC) or miR-92a construct-transfected (OE) HCT116 and A549 cells. Dual-Luciferase assay of (F) HCT116 and (G) A549 cells co-transfected with increasing plasmid ratios of NF2 −3′UTR-wild-type:miR-92a. (H) Secondary structure analysis of the RNA-RNA interaction (boxed region) between miR-92a-3p (red) and its MRE within wild-type vs. mutant NF2 −3′UTR (green). Dual-Luciferase assay of (I) HCT116 and (J) A549 cells co-transfected with wild-type or miR-92a-3p MRE mutant NF2 −3′UTR and miR-92a expression vectors. All experiments were performed in cells maintained in 0.5% serum. Data presented are representative of three independent trials and expressed as the mean ± standard deviation (SD). *P≤0.05, **P≤0.01, ***P≤0.001. NF2 , neurofibromin 2; UTR, untranslated region; MRE, microRNA response element.

    Techniques Used: Clone Assay, Sequencing, Luciferase, Transfection, Plasmid Preparation, Expressing, Construct, Mutagenesis, Standard Deviation

    Related Articles

    Amplification:

    Article Title: Distinct microRNA-155 expression in the vitreous of patients with primary vitreoretinal lymphoma and uveitis
    Article Snippet: The assays for microRNA-484, microRNA-197, microRNA-132, microRNA-155, microRNA-200c, microRNA-22* and the reference microRNA-16 were performed in the same plate in triplicate using StepOnePlus real-time PCR system (Life Technologies, ABI). .. To determine the Ct values, the threshold level of fluorescence was set manually in the early phase of PCR amplification.

    Article Title: Evaluation of MicroRNA Expression in Patient Bone Marrow Aspirate Slides
    Article Snippet: Quantitative Real-Time PCR Expression analysis used Applied Biosystems TaqMan® microRNA Assays for reverse transcription and Real-Time PCR (qRT-PCR) (Applied Biosystems® by Life Technologies, Mulgrave, Victoria AUST). .. Amplification was measured by the Applied Biosystems 7300 Sequence Detection System.

    Synthesized:

    Article Title: Distinct microRNA-155 expression in the vitreous of patients with primary vitreoretinal lymphoma and uveitis
    Article Snippet: The cDNA was synthesized by miScript II RT Kit (Qiagen). .. The assays for microRNA-484, microRNA-197, microRNA-132, microRNA-155, microRNA-200c, microRNA-22* and the reference microRNA-16 were performed in the same plate in triplicate using StepOnePlus real-time PCR system (Life Technologies, ABI).

    Construct:

    Article Title: LncRNA MACC1-AS1 sponges multiple miRNAs and RNA-binding protein PTBP1
    Article Snippet: .. Transfection of MACC1-AS1 constructs, miRNA mimics or siRNAs was conducted with Lipofectamine 2000 reagent (Invitrogen, USA) using the protocol recommended by the manufacturer. .. Lentivirus was generated by co-transfecting HEK-293T cells with the lentiviral vector and packaging plasmids as previously described .

    SYBR Green Assay:

    Article Title: Distinct microRNA-155 expression in the vitreous of patients with primary vitreoretinal lymphoma and uveitis
    Article Snippet: A master mix containing 2x QuantiTect SYBR Green PCR Master Mix, 10x miScript Universal Primer, 10x miScript Primer Assay, and RNase-free water was prepared for the Primer Assay specific to the microRNA of interest. .. The assays for microRNA-484, microRNA-197, microRNA-132, microRNA-155, microRNA-200c, microRNA-22* and the reference microRNA-16 were performed in the same plate in triplicate using StepOnePlus real-time PCR system (Life Technologies, ABI).

    Article Title: Pluripotency-associated miR-290/302 family of microRNAs promote the dismantling of naive pluripotency
    Article Snippet: RNA extraction, qRT-PCR and miRNA RT-PCR Total RNA was extracted following standard Trizol protocol (Invitrogen). .. Samples were centrifuged at > 12 000× g during precipitation and washing steps to preserve small RNAs. qPCR and miRNA RT-PCR were performed using Sybr Green mix (Applied Biosystems).

    Article Title: A gene regulatory network to control EMT programs in development and disease
    Article Snippet: Quantitative RT-PCR was performed using Fast SYBR Green Mastermix (Applied Biosystems), in a Step One Plus machine (Applied Biosystems) according to the manufacturers’ instructions. .. For mature miRNA expression assays, total RNA enriched for small RNAs was extracted using mir VanaTM miRNA Isolation Kit (Invitrogen), following the manufacturers’ instructions.

    Microarray:

    Article Title: MicroRNA-Based Separation of Cortico-Fugal Projection Neuron-Like Cells Derived From Embryonic Stem Cells
    Article Snippet: .. miRNA Microarray Analysis miRNA expression profiling was performed using the GeneChip miRNA4.0 Array and Flashtag Bundle (Affymetrix) according to the manufacturers’ protocols. .. Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng/8uL total RNA (Expression Analysis Technical Manual, miRNA 4.0 ARRAY & FLASHTAGTM BUNDLES, Affymetrix).

    Random Hexamer Labeling:

    Article Title: A gene regulatory network to control EMT programs in development and disease
    Article Snippet: For Reverse transcription cDNA synthesis, oligo (dT)18 and random hexamer primers with the Maxima First Strand cDNA Synthesis kit (Thermo Scientific) were used, following the manufacturers’ instructions. .. For mature miRNA expression assays, total RNA enriched for small RNAs was extracted using mir VanaTM miRNA Isolation Kit (Invitrogen), following the manufacturers’ instructions.

    Luciferase:

    Article Title: Interferon-? Induced microRNA-129-5p Down-Regulates HPV-18 E6 and E7 Viral Gene Expression by Targeting SP1 in Cervical Cancer Cells
    Article Snippet: Cells were transiently transfected with pre-miR-129-5p , non-specific miRNA control (pre-miR-negative) or blank control (Applied Biosystems) in Opti-MEM (Invitrogen, Carlsbad, CA) using siPORT NeoFX Transfection agent (Applied Biosystems) following the manufacturer's protocol. .. Luciferase reporter vector bearing the target sites for miR-129-5p of the SP1 3′-UTR region was purchased by Shanghai choseasy co., Ltd.

    Expressing:

    Article Title: LncRNA MACC1-AS1 sponges multiple miRNAs and RNA-binding protein PTBP1
    Article Snippet: Transfection of MACC1-AS1 constructs, miRNA mimics or siRNAs was conducted with Lipofectamine 2000 reagent (Invitrogen, USA) using the protocol recommended by the manufacturer. .. Expression levels of infected genes were detected by RT-qPCR or western blots.

    Article Title: Distinct microRNA-155 expression in the vitreous of patients with primary vitreoretinal lymphoma and uveitis
    Article Snippet: The assays for microRNA-484, microRNA-197, microRNA-132, microRNA-155, microRNA-200c, microRNA-22* and the reference microRNA-16 were performed in the same plate in triplicate using StepOnePlus real-time PCR system (Life Technologies, ABI). .. ABI SDS 1.3.1 software and the 2-ΔΔCt method was used to determine the relative microRNA level that was normalized first by the level of microRNA-16 from the same cDNA sample and again normalized to the microRNA level in the lower expression group.

    Article Title: Analysis of miRNA signatures in CSF identifies upregulation of miR-21 and miR-146a/b in patients with multiple sclerosis and active lesions
    Article Snippet: Analysis of individual miRNAs with TaqMan hydrolysis probes To study the presence of circulating miRNA candidates, the preamplification product was diluted (1:50 for CSF and 1:200 for plasma) and quantified with Individual TaqMan hydrolysis probes (Applied Biosystems). .. Individual gene expression was assessed by RT-PCR using the Light-Cycler® 480 System (Roche Molecular Biochemicals) and triplicates for each sample were performed.

    Article Title: A gene regulatory network to control EMT programs in development and disease
    Article Snippet: .. For mature miRNA expression assays, total RNA enriched for small RNAs was extracted using mir VanaTM miRNA Isolation Kit (Invitrogen), following the manufacturers’ instructions. .. Reverse transcription reactions were performed using TaqMan® MicroRNA Assay (Applied Biosystem) according to the manufacturers’ instructions.

    Article Title: MicroRNA-Based Separation of Cortico-Fugal Projection Neuron-Like Cells Derived From Embryonic Stem Cells
    Article Snippet: .. miRNA Microarray Analysis miRNA expression profiling was performed using the GeneChip miRNA4.0 Array and Flashtag Bundle (Affymetrix) according to the manufacturers’ protocols. .. Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng/8uL total RNA (Expression Analysis Technical Manual, miRNA 4.0 ARRAY & FLASHTAGTM BUNDLES, Affymetrix).

    Article Title: Evaluation of MicroRNA Expression in Patient Bone Marrow Aspirate Slides
    Article Snippet: .. Quantitative Real-Time PCR Expression analysis used Applied Biosystems TaqMan® microRNA Assays for reverse transcription and Real-Time PCR (qRT-PCR) (Applied Biosystems® by Life Technologies, Mulgrave, Victoria AUST). .. Amplification was measured by the Applied Biosystems 7300 Sequence Detection System.

    Modification:

    Article Title: Pluripotency-associated miR-290/302 family of microRNAs promote the dismantling of naive pluripotency
    Article Snippet: RNA extraction, qRT-PCR and miRNA RT-PCR Total RNA was extracted following standard Trizol protocol (Invitrogen). .. For miRNA qPCR, RNA samples were first polyadenylated with polyA polymerase and then reverse transcribed using a modified oligodT primer as described previously .

    Western Blot:

    Article Title: LncRNA MACC1-AS1 sponges multiple miRNAs and RNA-binding protein PTBP1
    Article Snippet: Transfection of MACC1-AS1 constructs, miRNA mimics or siRNAs was conducted with Lipofectamine 2000 reagent (Invitrogen, USA) using the protocol recommended by the manufacturer. .. Expression levels of infected genes were detected by RT-qPCR or western blots.

    Transfection:

    Article Title: Interferon-? Induced microRNA-129-5p Down-Regulates HPV-18 E6 and E7 Viral Gene Expression by Targeting SP1 in Cervical Cancer Cells
    Article Snippet: .. Cells were transiently transfected with pre-miR-129-5p , non-specific miRNA control (pre-miR-negative) or blank control (Applied Biosystems) in Opti-MEM (Invitrogen, Carlsbad, CA) using siPORT NeoFX Transfection agent (Applied Biosystems) following the manufacturer's protocol. .. Equal copy numbers of plasmids were transfected using Lipofectamine 2000 (Invitrogen) following the manufacturer's protocol.

    Article Title: MicroRNA-146a protects against myocardial ischaemia reperfusion injury by targeting Med1
    Article Snippet: Quantitative real-time PCR (qRT-PCR) of microRNA-146a and target genes Total RNA (including microRNA) extraction of H9C2 cells transfected with microRNA-146a inhibitor (miR20000852, RN:R10034.5, Ribobio, China) and myocardium at risk, including ischaemia and infarction tissues, using TRIZOL reagent (Qiagen) was performed in accordance with the manufacturer’s protocol, followed by reverse transcription with the Thermo Cycler machine (Applied Biosystems). qRT-PCR was conducted using a real-time PCR machine (LightCycler 480 II, Roche). .. The microRNA-146a level was quantified by qRT-PCR using a specific assays for microRNA (Applied Biosystems, USA) and TaqMan Universal Master Mix (Applied Biosystems, USA).

    Article Title: LncRNA MACC1-AS1 sponges multiple miRNAs and RNA-binding protein PTBP1
    Article Snippet: .. Transfection of MACC1-AS1 constructs, miRNA mimics or siRNAs was conducted with Lipofectamine 2000 reagent (Invitrogen, USA) using the protocol recommended by the manufacturer. .. Lentivirus was generated by co-transfecting HEK-293T cells with the lentiviral vector and packaging plasmids as previously described .

    Article Title: MicroRNA-139-3p regulates osteoblast differentiation and apoptosis by targeting ELK1 and interacting with long noncoding RNA ODSM
    Article Snippet: .. Cell transfection The miRNA regulators were transfected into cells using Lipofectamine 2000 (Invitrogen, USA). .. The concentration of miR-139-3p mimic and its negative control (RiboBio, China) for transfection was 50 nM, and the concentration of the inhibitor and its negative control (RiboBio, China) for transfection was 100 nM.

    Infection:

    Article Title: LncRNA MACC1-AS1 sponges multiple miRNAs and RNA-binding protein PTBP1
    Article Snippet: Paragraph title: Transfection, lentivirus production, and infection ... Transfection of MACC1-AS1 constructs, miRNA mimics or siRNAs was conducted with Lipofectamine 2000 reagent (Invitrogen, USA) using the protocol recommended by the manufacturer.

    TaqMan microRNA Assay:

    Article Title: A gene regulatory network to control EMT programs in development and disease
    Article Snippet: For mature miRNA expression assays, total RNA enriched for small RNAs was extracted using mir VanaTM miRNA Isolation Kit (Invitrogen), following the manufacturers’ instructions. .. Reverse transcription reactions were performed using TaqMan® MicroRNA Assay (Applied Biosystem) according to the manufacturers’ instructions.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Analysis of miRNA signatures in CSF identifies upregulation of miR-21 and miR-146a/b in patients with multiple sclerosis and active lesions
    Article Snippet: Analysis of individual miRNAs with TaqMan hydrolysis probes To study the presence of circulating miRNA candidates, the preamplification product was diluted (1:50 for CSF and 1:200 for plasma) and quantified with Individual TaqMan hydrolysis probes (Applied Biosystems). .. Individual gene expression was assessed by RT-PCR using the Light-Cycler® 480 System (Roche Molecular Biochemicals) and triplicates for each sample were performed.

    Article Title: Pluripotency-associated miR-290/302 family of microRNAs promote the dismantling of naive pluripotency
    Article Snippet: .. RNA extraction, qRT-PCR and miRNA RT-PCR Total RNA was extracted following standard Trizol protocol (Invitrogen). .. Samples were centrifuged at > 12 000× g during precipitation and washing steps to preserve small RNAs. qPCR and miRNA RT-PCR were performed using Sybr Green mix (Applied Biosystems).

    Generated:

    Article Title: LncRNA MACC1-AS1 sponges multiple miRNAs and RNA-binding protein PTBP1
    Article Snippet: Transfection of MACC1-AS1 constructs, miRNA mimics or siRNAs was conducted with Lipofectamine 2000 reagent (Invitrogen, USA) using the protocol recommended by the manufacturer. .. Lentivirus was generated by co-transfecting HEK-293T cells with the lentiviral vector and packaging plasmids as previously described .

    Polymerase Chain Reaction:

    Article Title: Distinct microRNA-155 expression in the vitreous of patients with primary vitreoretinal lymphoma and uveitis
    Article Snippet: A master mix containing 2x QuantiTect SYBR Green PCR Master Mix, 10x miScript Universal Primer, 10x miScript Primer Assay, and RNase-free water was prepared for the Primer Assay specific to the microRNA of interest. .. The assays for microRNA-484, microRNA-197, microRNA-132, microRNA-155, microRNA-200c, microRNA-22* and the reference microRNA-16 were performed in the same plate in triplicate using StepOnePlus real-time PCR system (Life Technologies, ABI).

    Article Title: A gene regulatory network to control EMT programs in development and disease
    Article Snippet: For mature miRNA expression assays, total RNA enriched for small RNAs was extracted using mir VanaTM miRNA Isolation Kit (Invitrogen), following the manufacturers’ instructions. .. For quantitative real-time retro-transcriptase PCR (qPCR), specific probes from TaqMan® MicroRNA Assay (Applied Biosystem) were used (Catalog # 4427975, hsa-miR-424-5p Assay ID: 000604 and hsa-miR-503–5p Assay ID: 001048).

    Fluorescence:

    Article Title: Distinct microRNA-155 expression in the vitreous of patients with primary vitreoretinal lymphoma and uveitis
    Article Snippet: The assays for microRNA-484, microRNA-197, microRNA-132, microRNA-155, microRNA-200c, microRNA-22* and the reference microRNA-16 were performed in the same plate in triplicate using StepOnePlus real-time PCR system (Life Technologies, ABI). .. To determine the Ct values, the threshold level of fluorescence was set manually in the early phase of PCR amplification.

    Multiple Displacement Amplification:

    Article Title: An Lmx1b-miR135a2 Regulatory Circuit Modulates Wnt1/Wnt Signaling and Determines the Size of the Midbrain Dopaminergic Progenitor Pool
    Article Snippet: .. miR135a2 is enriched in the ventral midbrain and embedded within a novel intron of Rmst In order to identify microRNAs (miRs) in the Wnt1 –rich mDA progenitor domain, ventral midline and dorsal lateral tissues were microdissected from 11.5 dpc mouse embryos and used to perform a microRNA array, followed by qRT-PCR validation with select individual TaqMan assays (Applied Biosystems). ..

    Isolation:

    Article Title: A gene regulatory network to control EMT programs in development and disease
    Article Snippet: .. For mature miRNA expression assays, total RNA enriched for small RNAs was extracted using mir VanaTM miRNA Isolation Kit (Invitrogen), following the manufacturers’ instructions. .. Reverse transcription reactions were performed using TaqMan® MicroRNA Assay (Applied Biosystem) according to the manufacturers’ instructions.

    RNA Extraction:

    Article Title: Pluripotency-associated miR-290/302 family of microRNAs promote the dismantling of naive pluripotency
    Article Snippet: .. RNA extraction, qRT-PCR and miRNA RT-PCR Total RNA was extracted following standard Trizol protocol (Invitrogen). .. Samples were centrifuged at > 12 000× g during precipitation and washing steps to preserve small RNAs. qPCR and miRNA RT-PCR were performed using Sybr Green mix (Applied Biosystems).

    Article Title: A gene regulatory network to control EMT programs in development and disease
    Article Snippet: Paragraph title: Total RNA extraction cDNA synthesis and qPCR analysis ... For mature miRNA expression assays, total RNA enriched for small RNAs was extracted using mir VanaTM miRNA Isolation Kit (Invitrogen), following the manufacturers’ instructions.

    Sequencing:

    Article Title: Evaluation of MicroRNA Expression in Patient Bone Marrow Aspirate Slides
    Article Snippet: Quantitative Real-Time PCR Expression analysis used Applied Biosystems TaqMan® microRNA Assays for reverse transcription and Real-Time PCR (qRT-PCR) (Applied Biosystems® by Life Technologies, Mulgrave, Victoria AUST). .. Amplification was measured by the Applied Biosystems 7300 Sequence Detection System.

    Quantitative RT-PCR:

    Article Title: MicroRNA-146a protects against myocardial ischaemia reperfusion injury by targeting Med1
    Article Snippet: .. The microRNA-146a level was quantified by qRT-PCR using a specific assays for microRNA (Applied Biosystems, USA) and TaqMan Universal Master Mix (Applied Biosystems, USA). .. Specific primers for microRNA-146a were obtained from Applied Biosystems [primer identification numbers: 000468 for microRNA-146a and 001973 for U6 small nuclear RNA (snRU6)]. mRNA of the possible target genes was quantified using Takara reverse transcription assay (R0037A) and SYGRII (RR820A).

    Article Title: LncRNA MACC1-AS1 sponges multiple miRNAs and RNA-binding protein PTBP1
    Article Snippet: Transfection of MACC1-AS1 constructs, miRNA mimics or siRNAs was conducted with Lipofectamine 2000 reagent (Invitrogen, USA) using the protocol recommended by the manufacturer. .. Expression levels of infected genes were detected by RT-qPCR or western blots.

    Article Title: Pluripotency-associated miR-290/302 family of microRNAs promote the dismantling of naive pluripotency
    Article Snippet: .. RNA extraction, qRT-PCR and miRNA RT-PCR Total RNA was extracted following standard Trizol protocol (Invitrogen). .. Samples were centrifuged at > 12 000× g during precipitation and washing steps to preserve small RNAs. qPCR and miRNA RT-PCR were performed using Sybr Green mix (Applied Biosystems).

    Article Title: An Lmx1b-miR135a2 Regulatory Circuit Modulates Wnt1/Wnt Signaling and Determines the Size of the Midbrain Dopaminergic Progenitor Pool
    Article Snippet: .. miR135a2 is enriched in the ventral midbrain and embedded within a novel intron of Rmst In order to identify microRNAs (miRs) in the Wnt1 –rich mDA progenitor domain, ventral midline and dorsal lateral tissues were microdissected from 11.5 dpc mouse embryos and used to perform a microRNA array, followed by qRT-PCR validation with select individual TaqMan assays (Applied Biosystems). ..

    Article Title: A gene regulatory network to control EMT programs in development and disease
    Article Snippet: Quantitative RT-PCR was performed using Fast SYBR Green Mastermix (Applied Biosystems), in a Step One Plus machine (Applied Biosystems) according to the manufacturers’ instructions. .. For mature miRNA expression assays, total RNA enriched for small RNAs was extracted using mir VanaTM miRNA Isolation Kit (Invitrogen), following the manufacturers’ instructions.

    Article Title: MicroRNA-146a protects against myocardial ischaemia reperfusion injury by targeting Med1
    Article Snippet: Paragraph title: Quantitative real-time PCR (qRT-PCR) of microRNA-146a and target genes ... Specific primers for microRNA-146a were obtained from Applied Biosystems [primer identification numbers: 000468 for microRNA-146a and 001973 for U6 small nuclear RNA (snRU6)]. mRNA of the possible target genes was quantified using Takara reverse transcription assay (R0037A) and SYGRII (RR820A).

    Article Title: Evaluation of MicroRNA Expression in Patient Bone Marrow Aspirate Slides
    Article Snippet: .. Quantitative Real-Time PCR Expression analysis used Applied Biosystems TaqMan® microRNA Assays for reverse transcription and Real-Time PCR (qRT-PCR) (Applied Biosystems® by Life Technologies, Mulgrave, Victoria AUST). .. Amplification was measured by the Applied Biosystems 7300 Sequence Detection System.

    Plasmid Preparation:

    Article Title: Interferon-? Induced microRNA-129-5p Down-Regulates HPV-18 E6 and E7 Viral Gene Expression by Targeting SP1 in Cervical Cancer Cells
    Article Snippet: Cells were transiently transfected with pre-miR-129-5p , non-specific miRNA control (pre-miR-negative) or blank control (Applied Biosystems) in Opti-MEM (Invitrogen, Carlsbad, CA) using siPORT NeoFX Transfection agent (Applied Biosystems) following the manufacturer's protocol. .. Luciferase reporter vector bearing the target sites for miR-129-5p of the SP1 3′-UTR region was purchased by Shanghai choseasy co., Ltd.

    Article Title: LncRNA MACC1-AS1 sponges multiple miRNAs and RNA-binding protein PTBP1
    Article Snippet: Transfection of MACC1-AS1 constructs, miRNA mimics or siRNAs was conducted with Lipofectamine 2000 reagent (Invitrogen, USA) using the protocol recommended by the manufacturer. .. Lentivirus was generated by co-transfecting HEK-293T cells with the lentiviral vector and packaging plasmids as previously described .

    Software:

    Article Title: Distinct microRNA-155 expression in the vitreous of patients with primary vitreoretinal lymphoma and uveitis
    Article Snippet: The assays for microRNA-484, microRNA-197, microRNA-132, microRNA-155, microRNA-200c, microRNA-22* and the reference microRNA-16 were performed in the same plate in triplicate using StepOnePlus real-time PCR system (Life Technologies, ABI). .. ABI SDS 1.3.1 software and the 2-ΔΔCt method was used to determine the relative microRNA level that was normalized first by the level of microRNA-16 from the same cDNA sample and again normalized to the microRNA level in the lower expression group.

    Real-time Polymerase Chain Reaction:

    Article Title: MicroRNA-146a protects against myocardial ischaemia reperfusion injury by targeting Med1
    Article Snippet: Paragraph title: Quantitative real-time PCR (qRT-PCR) of microRNA-146a and target genes ... The microRNA-146a level was quantified by qRT-PCR using a specific assays for microRNA (Applied Biosystems, USA) and TaqMan Universal Master Mix (Applied Biosystems, USA).

    Article Title: Distinct microRNA-155 expression in the vitreous of patients with primary vitreoretinal lymphoma and uveitis
    Article Snippet: .. The assays for microRNA-484, microRNA-197, microRNA-132, microRNA-155, microRNA-200c, microRNA-22* and the reference microRNA-16 were performed in the same plate in triplicate using StepOnePlus real-time PCR system (Life Technologies, ABI). ..

    Article Title: Pluripotency-associated miR-290/302 family of microRNAs promote the dismantling of naive pluripotency
    Article Snippet: RNA extraction, qRT-PCR and miRNA RT-PCR Total RNA was extracted following standard Trizol protocol (Invitrogen). .. Samples were centrifuged at > 12 000× g during precipitation and washing steps to preserve small RNAs. qPCR and miRNA RT-PCR were performed using Sybr Green mix (Applied Biosystems).

    Article Title: A gene regulatory network to control EMT programs in development and disease
    Article Snippet: Paragraph title: Total RNA extraction cDNA synthesis and qPCR analysis ... For mature miRNA expression assays, total RNA enriched for small RNAs was extracted using mir VanaTM miRNA Isolation Kit (Invitrogen), following the manufacturers’ instructions.

    Article Title: Evaluation of MicroRNA Expression in Patient Bone Marrow Aspirate Slides
    Article Snippet: .. Quantitative Real-Time PCR Expression analysis used Applied Biosystems TaqMan® microRNA Assays for reverse transcription and Real-Time PCR (qRT-PCR) (Applied Biosystems® by Life Technologies, Mulgrave, Victoria AUST). .. Amplification was measured by the Applied Biosystems 7300 Sequence Detection System.

    Negative Control:

    Article Title: MicroRNA-139-3p regulates osteoblast differentiation and apoptosis by targeting ELK1 and interacting with long noncoding RNA ODSM
    Article Snippet: Cell transfection The miRNA regulators were transfected into cells using Lipofectamine 2000 (Invitrogen, USA). .. The concentration of miR-139-3p mimic and its negative control (RiboBio, China) for transfection was 50 nM, and the concentration of the inhibitor and its negative control (RiboBio, China) for transfection was 100 nM.

    RNA Expression:

    Article Title: MicroRNA-Based Separation of Cortico-Fugal Projection Neuron-Like Cells Derived From Embryonic Stem Cells
    Article Snippet: miRNA Microarray Analysis miRNA expression profiling was performed using the GeneChip miRNA4.0 Array and Flashtag Bundle (Affymetrix) according to the manufacturers’ protocols. .. Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng/8uL total RNA (Expression Analysis Technical Manual, miRNA 4.0 ARRAY & FLASHTAGTM BUNDLES, Affymetrix).

    Acid Assay:

    Article Title: Distinct microRNA-155 expression in the vitreous of patients with primary vitreoretinal lymphoma and uveitis
    Article Snippet: Paragraph title: Individual micro Ribonucleic Acid assay ... The assays for microRNA-484, microRNA-197, microRNA-132, microRNA-155, microRNA-200c, microRNA-22* and the reference microRNA-16 were performed in the same plate in triplicate using StepOnePlus real-time PCR system (Life Technologies, ABI).

    Concentration Assay:

    Article Title: MicroRNA-139-3p regulates osteoblast differentiation and apoptosis by targeting ELK1 and interacting with long noncoding RNA ODSM
    Article Snippet: Cell transfection The miRNA regulators were transfected into cells using Lipofectamine 2000 (Invitrogen, USA). .. The concentration of miR-139-3p mimic and its negative control (RiboBio, China) for transfection was 50 nM, and the concentration of the inhibitor and its negative control (RiboBio, China) for transfection was 100 nM.

    Standard Deviation:

    Article Title: Evaluation of MicroRNA Expression in Patient Bone Marrow Aspirate Slides
    Article Snippet: Quantitative Real-Time PCR Expression analysis used Applied Biosystems TaqMan® microRNA Assays for reverse transcription and Real-Time PCR (qRT-PCR) (Applied Biosystems® by Life Technologies, Mulgrave, Victoria AUST). .. Replicates were omitted if threshold cycle (Ct ) standard deviation greater than 1, or where Ct values were exactly 40.