quant it picogreen dsdna broad range assay kit  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Quant iT PicoGreen dsDNA Assay Kit
    Description:
    Using the PicoGreen dsDNA quantitation assay you can selectively detect as little as 25 pg ml of dsDNA in the presence of ssDNA RNA and free nucleotides The assay is linear over three orders of magnitude and has little sequence dependence allowing you to accurately measure DNA from many sources including genomic DNA viral DNA miniprep DNA or PCR amplification products PicoGreen dsDNA Quantitation Reagents are • Orders of magnitude more sensitive than UV absorbance readings saving on precious sample Figure 1 • Specific for dsDNA in the presence of equimolar amounts of RNA Figure 2 • Easy to use just add the dye to sample wait 5 minutes and read• Suitable for use in 96 and 384 well plate formats• Compatible with most fluorescence based microplate readers and fluorometers and FluorescenceApplications The PicoGreen dsDNA Quantitation Reagent and Kits are ideal for PCR based assays microarray samples DNA damage assays enzyme activity assays genomic DNA quantitation measuring dsDNA in complex mixtures and viral DNA quantitation
    Catalog Number:
    p11496
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|DNA Quantitation|Nucleic Acid Quantitation
    Category:
    Kits and Assays
    Buy from Supplier


    Structured Review

    Thermo Fisher quant it picogreen dsdna broad range assay kit
    Using the PicoGreen dsDNA quantitation assay you can selectively detect as little as 25 pg ml of dsDNA in the presence of ssDNA RNA and free nucleotides The assay is linear over three orders of magnitude and has little sequence dependence allowing you to accurately measure DNA from many sources including genomic DNA viral DNA miniprep DNA or PCR amplification products PicoGreen dsDNA Quantitation Reagents are • Orders of magnitude more sensitive than UV absorbance readings saving on precious sample Figure 1 • Specific for dsDNA in the presence of equimolar amounts of RNA Figure 2 • Easy to use just add the dye to sample wait 5 minutes and read• Suitable for use in 96 and 384 well plate formats• Compatible with most fluorescence based microplate readers and fluorometers and FluorescenceApplications The PicoGreen dsDNA Quantitation Reagent and Kits are ideal for PCR based assays microarray samples DNA damage assays enzyme activity assays genomic DNA quantitation measuring dsDNA in complex mixtures and viral DNA quantitation
    https://www.bioz.com/result/quant it picogreen dsdna broad range assay kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quant it picogreen dsdna broad range assay kit - by Bioz Stars, 2020-04
    99/100 stars

    Related Products / Commonly Used Together

    ϕx174 dsdna replicative

    Images

    Related Articles

    Irradiation:

    Article Title: Oxidized mitochondrial nucleoids released by neutrophils drive type I interferon production in human lupus
    Article Snippet: DNA concentration was assessed with Quant-iT Picogreen dsDNA Assay kit (Invitrogen). .. For dot blot assay, 5 ng of DNA was blotted on a positively charged nylon membrane using the Bio-Dot Microfiltration System (Bio-Rad Laboratories), and then cross-linked by UV irradiation for 3 min.

    Fluorescence:

    Article Title: Kidney Decellularized Extracellular Matrix Hydrogels: Rheological Characterization and Human Glomerular Endothelial Cell Response to Encapsulation
    Article Snippet: DNA was quantified with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, #P7589) following the manufacturer’s protocol and a dsDNA standard included in the kit to generate a standard curve. .. Fluorescence was measured on a BioTek Cytation 3 Multi-Mode Reader with an excitation wavelength of 480 nm and emission wavelength of 520 nm.

    Article Title: Collagen and Hydroxyapatite Scaffolds Activate Distinct Osteogenesis Signaling Pathways in Adult Adipose-Derived Multipotent Stromal Cells
    Article Snippet: Total dsDNA was quantified using a commercially available kit (Quant-iT PicoGreen dsDNA Assay Kit; Thermo Fisher Scientific). .. Fluorescence was measured at 480 and 520 nm and dsDNA quantified on a lambda DNA standard curve.

    Article Title: A Non-woven Path: Electrospun Poly(lactic acid) Scaffolds for Kidney Tissue Engineering
    Article Snippet: Total DNA content of the samples was calculated using a Quant-iT™ PicoGreen® assay kit (ThermoFisher, UK) as per the manufacturers’ instructions. .. Fluorescence was read using a microplate reader at Ex 490 nm Em 510–570 nm, N = 4 independent replicates.

    Colorimetric Assay:

    Article Title: Periodic mild heat stimuli diminish extracellular matrix synthesis in pellet cultured human chondrocytes
    Article Snippet: For quantitative comparison, collagen and proteoglycan content were measured using the hydroxyproline assay [ ] for collagen content and the 1,9-dimethylmethylene blue (DMMB) colorimetric assay for proteoglycan content ( n = 6 pellets/group) [ ]. .. In order to estimate the cell amount in the pellet, DNA content (n = 6 pellets/group) was measured by the Quant-iT™ PicoGreen® assay (Invitrogen Ltd., Paisley, UK) following the manufacturer’s instructions.

    Isolation:

    Article Title: Oxidized mitochondrial nucleoids released by neutrophils drive type I interferon production in human lupus
    Article Snippet: Paragraph title: Isolation of DNA from supernatants and analysis ... DNA concentration was assessed with Quant-iT Picogreen dsDNA Assay kit (Invitrogen).

    Picogreen Assay:

    Article Title: Growth factor and ultrasound-assisted bioreactor synergism for human mesenchymal stem cell chondrogenesis
    Article Snippet: .. Double-stranded DNA (dsDNA) was measured using Quant-iT™ PicoGreen® dsDNA Assay Kit (Life Technologies) according to manufacturer’s instructions. ..

    Article Title: Oxidized mitochondrial nucleoids released by neutrophils drive type I interferon production in human lupus
    Article Snippet: .. DNA concentration was assessed with Quant-iT Picogreen dsDNA Assay kit (Invitrogen). .. PCR was performed with 5 ng of isolated DNA, AmpliTaq Gold 360 (Invitrogen) and 0.5 µM of the following primers: mtDNA encoded NADH dehydrogenase subunit 1 (ND1; 5′-GCATTCCTAATGCTTACCGAAC-3′ and 5′-AAGGGTGGAGAGGTTAAAGGAG-3′); genomic DNA encoded Glyceraldehyde 3- phosphate dehydrogenase (GAPDH; 5′-AGGCAACTAGGATGGTGTGG-3′ and 5′-TTGATTTTGGAGGGATCTCG-3′).

    Article Title: Lentiviral Vector Purification Using Genetically Encoded Biotin Mimic in Packaging Cell
    Article Snippet: .. DNA Quantification Double-stranded DNA contamination was assessed in purified vectors using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen). .. The manufacturers’ protocol was followed using a Thermo Scientific Nunc F96 MicroWell black polystyrene plate.

    Article Title: Periodic mild heat stimuli diminish extracellular matrix synthesis in pellet cultured human chondrocytes
    Article Snippet: .. In order to estimate the cell amount in the pellet, DNA content (n = 6 pellets/group) was measured by the Quant-iT™ PicoGreen® assay (Invitrogen Ltd., Paisley, UK) following the manufacturer’s instructions. ..

    Article Title: Kidney Decellularized Extracellular Matrix Hydrogels: Rheological Characterization and Human Glomerular Endothelial Cell Response to Encapsulation
    Article Snippet: .. DNA was quantified with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, #P7589) following the manufacturer’s protocol and a dsDNA standard included in the kit to generate a standard curve. .. Fluorescence was measured on a BioTek Cytation 3 Multi-Mode Reader with an excitation wavelength of 480 nm and emission wavelength of 520 nm.

    Article Title: Development of a Cytocompatible Scaffold from Pig Immature Testicular Tissue Allowing Human Sertoli Cell Attachment, Proliferation and Functionality
    Article Snippet: .. For quantification, DNA was extracted using the PureLink™ genomic DNA mini kit (Thermo Fisher, Ghent, Belgium, K1820-01) and quantified using the Quant-iT™ PicoGreen® dsDNA assay kit (Thermo Fisher, P7589) following the manufacturer’s instructions. .. Preservation of fibronectin, collagen IV and laminin were evaluated by immunohistochemistry.

    Article Title: Collagen and Hydroxyapatite Scaffolds Activate Distinct Osteogenesis Signaling Pathways in Adult Adipose-Derived Multipotent Stromal Cells
    Article Snippet: .. Total dsDNA was quantified using a commercially available kit (Quant-iT PicoGreen dsDNA Assay Kit; Thermo Fisher Scientific). .. Fluorescence was measured at 480 and 520 nm and dsDNA quantified on a lambda DNA standard curve.

    Article Title: NET formation can occur independently of RIPK3 and MLKL signaling
    Article Snippet: .. Quant‐iTTM PicoGreen® dsDNA Assay Kit, propidium iodide (PI), Hanks balanced salt solution (HBSS), RPMI‐1640/GlutaMAX medium, penicillin/streptomycin as well as Alexa Fluor® 488‐conjugated anti‐mouse and anti‐goat secondary antibodies were obtained from ThermoFisher scientific (distributed by LuBioScience GmbH, Lucerne, Switzerland). .. Human TNF‐α and mouse GM‐CSF were purchased from R & D Systems (Abingdon, UK) and z‐VAD‐FMK (zVAD) from BD Biosciences (Erembodegem, Belgium).

    Article Title: Neutrophil activation and NETosis are the major drivers of thrombosis in heparin-induced thrombocytopenia
    Article Snippet: .. Quantification of NETosis markers Plasma levels of cfDNA were measured using a fluorometric assay for double-stranded DNA Quant-iT PicoGreen dsDNA Assay kit (Invitrogen) following the manufacturer’s instructions. .. Plasma levels of citrullinated histone H3, human myeloperoxidase, neutrophil elastase and VWF were determined by ELISA with Citrullinated Histone H3 (Clone 11D3) ELISA Kit (Cayman Chemicals), Human Myeloperoxidase Quantikine ELISA Kit (R & D Systems), Human PMN Elastase Platinum ELISA (eBioscience, San Diego, CA) or by in-house ELISA using sheep anti-human VWF (Abcam, ab11713) for coating followed by detection with polyclonal rabbit anti-human VWF-HRP (Dako, P0226), respectively.

    Article Title: A Non-woven Path: Electrospun Poly(lactic acid) Scaffolds for Kidney Tissue Engineering
    Article Snippet: .. Total DNA content of the samples was calculated using a Quant-iT™ PicoGreen® assay kit (ThermoFisher, UK) as per the manufacturers’ instructions. .. Fluorescence was read using a microplate reader at Ex 490 nm Em 510–570 nm, N = 4 independent replicates.

    Article Title: Matrix-bound nanovesicles within ECM bioscaffolds
    Article Snippet: .. The proteinase K solution, Quant-iT PicoGreen dsDNA Assay Kit, and RNase A were obtained from Thermo Fisher Scientific. .. RNase-free DNase was obtained from Qiagen.

    Lambda DNA Preparation:

    Article Title: Collagen and Hydroxyapatite Scaffolds Activate Distinct Osteogenesis Signaling Pathways in Adult Adipose-Derived Multipotent Stromal Cells
    Article Snippet: Total dsDNA was quantified using a commercially available kit (Quant-iT PicoGreen dsDNA Assay Kit; Thermo Fisher Scientific). .. Fluorescence was measured at 480 and 520 nm and dsDNA quantified on a lambda DNA standard curve.

    dsDNA Assay:

    Article Title: Pullulan: a new cytoadhesive for cell-mediated cartilage repair
    Article Snippet: .. The Quant-iT PicoGreen double stranded DNA (dsDNA) assay kit (Molecular Probes, Life Technologies, Paisley, UK) was utilised according to the manufacturer’s protocol. .. Samples were evaluated on a Wallac Victor™ 1420 Multilabel Counter spectrophotometer.

    Spectrophotometry:

    Article Title: Pullulan: a new cytoadhesive for cell-mediated cartilage repair
    Article Snippet: Culture media absorbance was evaluated at 490 nm on a Wallac Victor™ 1420 Multilabel Counter spectrophotometer. .. The Quant-iT PicoGreen double stranded DNA (dsDNA) assay kit (Molecular Probes, Life Technologies, Paisley, UK) was utilised according to the manufacturer’s protocol.

    Purification:

    Article Title: Lentiviral Vector Purification Using Genetically Encoded Biotin Mimic in Packaging Cell
    Article Snippet: .. DNA Quantification Double-stranded DNA contamination was assessed in purified vectors using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen). .. The manufacturers’ protocol was followed using a Thermo Scientific Nunc F96 MicroWell black polystyrene plate.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Neutrophil activation and NETosis are the major drivers of thrombosis in heparin-induced thrombocytopenia
    Article Snippet: Quantification of NETosis markers Plasma levels of cfDNA were measured using a fluorometric assay for double-stranded DNA Quant-iT PicoGreen dsDNA Assay kit (Invitrogen) following the manufacturer’s instructions. .. Plasma levels of citrullinated histone H3, human myeloperoxidase, neutrophil elastase and VWF were determined by ELISA with Citrullinated Histone H3 (Clone 11D3) ELISA Kit (Cayman Chemicals), Human Myeloperoxidase Quantikine ELISA Kit (R & D Systems), Human PMN Elastase Platinum ELISA (eBioscience, San Diego, CA) or by in-house ELISA using sheep anti-human VWF (Abcam, ab11713) for coating followed by detection with polyclonal rabbit anti-human VWF-HRP (Dako, P0226), respectively.

    Concentration Assay:

    Article Title: Oxidized mitochondrial nucleoids released by neutrophils drive type I interferon production in human lupus
    Article Snippet: .. DNA concentration was assessed with Quant-iT Picogreen dsDNA Assay kit (Invitrogen). .. PCR was performed with 5 ng of isolated DNA, AmpliTaq Gold 360 (Invitrogen) and 0.5 µM of the following primers: mtDNA encoded NADH dehydrogenase subunit 1 (ND1; 5′-GCATTCCTAATGCTTACCGAAC-3′ and 5′-AAGGGTGGAGAGGTTAAAGGAG-3′); genomic DNA encoded Glyceraldehyde 3- phosphate dehydrogenase (GAPDH; 5′-AGGCAACTAGGATGGTGTGG-3′ and 5′-TTGATTTTGGAGGGATCTCG-3′).

    Incubation:

    Article Title: Growth factor and ultrasound-assisted bioreactor synergism for human mesenchymal stem cell chondrogenesis
    Article Snippet: Upon completion of the incubation, scaffolds were spun down, and supernatants were collected and subjected to DNA, GAG, and Hydroxyproline content measurements. .. Double-stranded DNA (dsDNA) was measured using Quant-iT™ PicoGreen® dsDNA Assay Kit (Life Technologies) according to manufacturer’s instructions.

    Article Title: A Non-woven Path: Electrospun Poly(lactic acid) Scaffolds for Kidney Tissue Engineering
    Article Snippet: DNA quantification Scaffolds were washed 3 times in PBS and freeze dried for 24 h. Each scaffold was incubated within an ultrasonic bath (6 litre Cavitek Digital, The Allendale Group, UK) in 300 µl of a 2.5 units/ml papain digest solution, 5 mM cysteine HCL and 5 mM EDTA in PBS (all reagents from Sigma Aldrich, UK) at 60 °C for 24 h with periodic sonication. .. Total DNA content of the samples was calculated using a Quant-iT™ PicoGreen® assay kit (ThermoFisher, UK) as per the manufacturers’ instructions.

    Article Title: Pullulan: a new cytoadhesive for cell-mediated cartilage repair
    Article Snippet: MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium), was applied according to the manufacturer’s instructions (Promega, Southampton, UK) and the culture incubated for two hours at 37°C. .. The Quant-iT PicoGreen double stranded DNA (dsDNA) assay kit (Molecular Probes, Life Technologies, Paisley, UK) was utilised according to the manufacturer’s protocol.

    Dot Blot:

    Article Title: Oxidized mitochondrial nucleoids released by neutrophils drive type I interferon production in human lupus
    Article Snippet: DNA concentration was assessed with Quant-iT Picogreen dsDNA Assay kit (Invitrogen). .. For dot blot assay, 5 ng of DNA was blotted on a positively charged nylon membrane using the Bio-Dot Microfiltration System (Bio-Rad Laboratories), and then cross-linked by UV irradiation for 3 min.

    Dimethylmethylene Blue Assay:

    Article Title: Growth factor and ultrasound-assisted bioreactor synergism for human mesenchymal stem cell chondrogenesis
    Article Snippet: Double-stranded DNA (dsDNA) was measured using Quant-iT™ PicoGreen® dsDNA Assay Kit (Life Technologies) according to manufacturer’s instructions. .. Total GAG content was determined with DMMB assay on papain-digested supernatant according to the protocol described elsewhere and quantified based on shark chondroitin sulfate standard.

    Staining:

    Article Title: Periodic mild heat stimuli diminish extracellular matrix synthesis in pellet cultured human chondrocytes
    Article Snippet: The pellets were subjected to macrophotography and wet weight measurement ( n = 12 pellets/group).The pellets were then used for picrosirius red staining to visualize collagen, and for safranin-O staining to assess proteoglycan deposition ( n = 6 pellets/group). .. In order to estimate the cell amount in the pellet, DNA content (n = 6 pellets/group) was measured by the Quant-iT™ PicoGreen® assay (Invitrogen Ltd., Paisley, UK) following the manufacturer’s instructions.

    Article Title: Development of a Cytocompatible Scaffold from Pig Immature Testicular Tissue Allowing Human Sertoli Cell Attachment, Proliferation and Functionality
    Article Snippet: Sections of native tissue and DT were stained with H and E or Masson’s trichrome to evaluate the presence of nuclear residues and general tissue morphology. .. For quantification, DNA was extracted using the PureLink™ genomic DNA mini kit (Thermo Fisher, Ghent, Belgium, K1820-01) and quantified using the Quant-iT™ PicoGreen® dsDNA assay kit (Thermo Fisher, P7589) following the manufacturer’s instructions.

    Polymerase Chain Reaction:

    Article Title: Oxidized mitochondrial nucleoids released by neutrophils drive type I interferon production in human lupus
    Article Snippet: DNA concentration was assessed with Quant-iT Picogreen dsDNA Assay kit (Invitrogen). .. PCR was performed with 5 ng of isolated DNA, AmpliTaq Gold 360 (Invitrogen) and 0.5 µM of the following primers: mtDNA encoded NADH dehydrogenase subunit 1 (ND1; 5′-GCATTCCTAATGCTTACCGAAC-3′ and 5′-AAGGGTGGAGAGGTTAAAGGAG-3′); genomic DNA encoded Glyceraldehyde 3- phosphate dehydrogenase (GAPDH; 5′-AGGCAACTAGGATGGTGTGG-3′ and 5′-TTGATTTTGGAGGGATCTCG-3′).

    Sonication:

    Article Title: A Non-woven Path: Electrospun Poly(lactic acid) Scaffolds for Kidney Tissue Engineering
    Article Snippet: DNA quantification Scaffolds were washed 3 times in PBS and freeze dried for 24 h. Each scaffold was incubated within an ultrasonic bath (6 litre Cavitek Digital, The Allendale Group, UK) in 300 µl of a 2.5 units/ml papain digest solution, 5 mM cysteine HCL and 5 mM EDTA in PBS (all reagents from Sigma Aldrich, UK) at 60 °C for 24 h with periodic sonication. .. Total DNA content of the samples was calculated using a Quant-iT™ PicoGreen® assay kit (ThermoFisher, UK) as per the manufacturers’ instructions.

    Hydroxyproline Assay:

    Article Title: Growth factor and ultrasound-assisted bioreactor synergism for human mesenchymal stem cell chondrogenesis
    Article Snippet: Double-stranded DNA (dsDNA) was measured using Quant-iT™ PicoGreen® dsDNA Assay Kit (Life Technologies) according to manufacturer’s instructions. .. Hydroxyproline content was measured on the papain-digested supernatant accordingly using Hydroxyproline Assay Kit (Sigma) according to manufacturer’s instructions.

    Article Title: Periodic mild heat stimuli diminish extracellular matrix synthesis in pellet cultured human chondrocytes
    Article Snippet: For quantitative comparison, collagen and proteoglycan content were measured using the hydroxyproline assay [ ] for collagen content and the 1,9-dimethylmethylene blue (DMMB) colorimetric assay for proteoglycan content ( n = 6 pellets/group) [ ]. .. In order to estimate the cell amount in the pellet, DNA content (n = 6 pellets/group) was measured by the Quant-iT™ PicoGreen® assay (Invitrogen Ltd., Paisley, UK) following the manufacturer’s instructions.

    Transmission Electron Microscopy:

    Article Title: Matrix-bound nanovesicles within ECM bioscaffolds
    Article Snippet: The proteinase K solution, Quant-iT PicoGreen dsDNA Assay Kit, and RNase A were obtained from Thermo Fisher Scientific. .. All reagents were assessed by TEM to ensure that they were free of contaminating EVs.

    Software:

    Article Title: Lentiviral Vector Purification Using Genetically Encoded Biotin Mimic in Packaging Cell
    Article Snippet: DNA Quantification Double-stranded DNA contamination was assessed in purified vectors using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen). .. Absorbance was measured using the Varioskan LUX multimode microplate reader and analyzed using the SkanIt software.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher picogreen fluorescent dye
    Picogreen Fluorescent Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/picogreen fluorescent dye/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    picogreen fluorescent dye - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results