Quadrupole Time Of Flight Tripletof 5600 Mass Spectrometer, supplied by SCIEX, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Label-Free Quantitation and Mapping of the ErbB2 Tumor Receptor by Multiple Protease Digestion with Data-Dependent (MS1) and Data-Independent (MS2) Acquisitions"
Article Title: Label-Free Quantitation and Mapping of the ErbB2 Tumor Receptor by Multiple Protease Digestion with Data-Dependent (MS1) and Data-Independent (MS2) Acquisitions
Journal: International Journal of Proteomics
Figure Legend Snippet: Workflow for ErbB2 targeted proteomics using multiprotease digestion and high resolution mass spectrometry quantitation. ErbB2 immunopurified from four 15 cm plates of untreated SK-BR-3 cells was pooled into a single sample. The sample was split into 12 aliquots and separated by SDS-PAGE. Triplicate in-gel digestion was performed using either trypsin, Asp-N, chymotrypsin, or a double digestion with trypsin plus Asp-N. Each sample was analyzed using an AB SCIEX TripleTOF 5600 mass spectrometer. Two approaches for high resolution LC-MS/MS quantitation were employed, MS1 Filtering and SWATH MS2 acquisition.
Techniques Used: Mass Spectrometry, Quantitation Assay, SDS Page, Liquid Chromatography with Mass Spectroscopy
2) Product Images from "Platform-independent and Label-free Quantitation of Proteomic Data Using MS1 Extracted Ion Chromatograms in Skyline"
Article Title: Platform-independent and Label-free Quantitation of Proteomic Data Using MS1 Extracted Ion Chromatograms in Skyline
Journal: Molecular & Cellular Proteomics : MCP
Figure Legend Snippet: Standard concentration curves for stable isotope-labeled and acetyllysine containing peptides. A and B , YAPVA Kac DLAS R ( A , succinate dehydrogenase complex, subunit A) and LVSSVSDLP KacR ( B , 3-hydroxy-3-methylglutaryl-CoA synthase 2) spanning a concentration range from 4 attomoles to 25 femtomoles. Peptides were diluted into either a simple matrix ( red triangles , 25 fmol “six protein mix”) or a complex matrix ( blue circles , mitochondrial lysate from mouse liver, 0.3 μg on column), and acquired on a TripleTOF 5600 mass spectrometer in triplicates. MS1 filtered peak area curves for precursor ions M with m/z 621.8395 2+ ( A , heavy YAPVA Kac DLAS R ) and m/z 626.8604 2+ ( B , heavy LVSSVSDLP KacR ). Weighted regression lines and calculated LOQ are indicated (also see supplemental Table S3 ), and weighted linear regression slopes were determined as 1.03 and 1.03 for YAP and 0.99 and 1.00 for LVS peptides in the different matrices. C , peak area CVs across three replicates for each concentration point (0.037–25 fmol) in complex matrix are shown for six targeted acetyllysine peptides with 20 and 30% CV cutoffs indicated (also see supplemental Figs. S6, C and F ). D , high throughput analysis showing peak area CVs for 366 peptides across 27 independently acquired runs in a complex mitochondrial lysate background matrix. Peak area CVs are displayed against MS1 filtered precursor m/z .
Techniques Used: Concentration Assay, Labeling, Mass Spectrometry, High Throughput Screening Assay