qtrap 3200  (SCIEX)

Journal: Allergy, Asthma & Immunology Research

Article Title: Dupilumab Therapy Improves Stratum Corneum Hydration and Skin Dysbiosis in Patients With Atopic Dermatitis

doi: 10.4168/aair.2021.13.5.762

Figure Lengend Snippet: Changes in skin barrier function and SC ceramide content in patients with AD following dupilumab treatment. (A) Changes in TEWL, SC hydration, and skin surface pH in lesional and non-lesional skin from baseline to week 12. (B) EASI scores before and after treatment. (C) Correlation between the percentage change in SC hydration levels from baseline to week 12 and the percentage decline in EASI scores at week 12. (D) Liquid chromatography electrospray ionization tandem mass spectrometry analysis of the ceramide levels in SC of the lesional and non-lesional skin before and after dupilumab treatment. (E) Correlation between the percentage changes in C26 ceramide contents and SC hydration levels from baseline to week 12 in the lesional skin. Data are expressed as mean ± standard error of mean. SC, stratum corneum; TEWL, transepidermal water loss; EASI, eczema area and severity index; B, baseline; 3M, 3 months of treatment; AD, atopic dermatitis. * P

Article Snippet: The extracted lipids dried using a vacuum system (Vision, Seoul, Korea) were re-dissolved in methanol and analyzed by liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS, API 3200 QTRAP mass; AB/SCIEX, Framingham, MA, USA) by selective ion monitoring mode.

Techniques: Liquid Chromatography, Mass Spectrometry

Journal:

Article Title: Distinct Isoforms of Phospholipase A2 Mediate the Ability of Salmonella enterica Serotype Typhimurium and Shigella flexneri To Induce the Transepithelial Migration of Neutrophils ▿

doi: 10.1128/IAI.00407-08

Figure Lengend Snippet: Identification of HXA 3 in the apical supernatants of T-84 monolayers. HXA 3 was identified by employing a triple quadrupole LC/MS-MS system as described in Materials and Methods. (A) Diagnostic fragment ions were identified by MS-MS enhanced-product analyses

Article Snippet: Extracted samples suspended in methanol were analyzed by a triple quadrupole linear ion trap LC/MS-MS system (MDS Sciex 3200 QTRAP; Applied Biosystems, Foster City, CA) equipped with a LUNA C18(2) minibore column (Phenomenex, Torrance, CA) using a mobile phase (methanol:water:acetate, 65:35:0.03 [vol/vol/vol]) with a 0.35-ml/flow rate.

Techniques: Liquid Chromatography with Mass Spectroscopy, Diagnostic Assay, Tandem Mass Spectroscopy

Journal: International Journal of Molecular Sciences

Article Title: Diesel Particulate Extract Accelerates Premature Skin Aging in Human Fibroblasts via Ceramide-1-Phosphate-Mediated Signaling Pathway

doi: 10.3390/ijms23052691

Figure Lengend Snippet: NADPH Oxidase activity. HDF were incubated with DPE or vehicle for 24 h. Activity of NAPDH oxidases (NOX) was determined by measuring the ratio of NADP + to NADPH with LC-ESI-MS/MS (API 3200 QTRAP mass, AB/SCIEX) by multiple-reaction monitoring mode (MRM). All values are mean ± SD ( n = 3). * p

Article Snippet: NADPH oxidases activity was measured by the ratio of NADP+ to NADPH using LC-ESI-MS/MS (API 3200 QTRAP mass, AB/SCIEX, Framingham, MA, USA) by multiple reaction monitoring mode, as we described previously [ ].

Techniques: Activity Assay, Incubation, Tandem Mass Spectroscopy