Journal: Allergy, Asthma & Immunology Research
Article Title: Dupilumab Therapy Improves Stratum Corneum Hydration and Skin Dysbiosis in Patients With Atopic Dermatitis
Figure Lengend Snippet: Changes in skin barrier function and SC ceramide content in patients with AD following dupilumab treatment. (A) Changes in TEWL, SC hydration, and skin surface pH in lesional and non-lesional skin from baseline to week 12. (B) EASI scores before and after treatment. (C) Correlation between the percentage change in SC hydration levels from baseline to week 12 and the percentage decline in EASI scores at week 12. (D) Liquid chromatography electrospray ionization tandem mass spectrometry analysis of the ceramide levels in SC of the lesional and non-lesional skin before and after dupilumab treatment. (E) Correlation between the percentage changes in C26 ceramide contents and SC hydration levels from baseline to week 12 in the lesional skin. Data are expressed as mean ± standard error of mean. SC, stratum corneum; TEWL, transepidermal water loss; EASI, eczema area and severity index; B, baseline; 3M, 3 months of treatment; AD, atopic dermatitis. * P
Article Snippet: The extracted lipids dried using a vacuum system (Vision, Seoul, Korea) were re-dissolved in methanol and analyzed by liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS, API 3200 QTRAP mass; AB/SCIEX, Framingham, MA, USA) by selective ion monitoring mode.
Techniques: Liquid Chromatography, Mass Spectrometry