Structured Review

Roche qrt pcr roche lightcycler 480 system
Immune responses of CiIFN1/3 to plasmids containing CpG ODNs Left, the mRNA expressions of CiIFN1 (A) and CiIFN3 (B) were measured at 24 and 48 h post-infection. CIK cells were stimulated with PBS (control) or plasmids containing CpG ODNs and infected with GCRV. Other captions were the same as Figure 3 . Right, CIK cells were co-transfected with 800 ng of pRL-TK and IFN1pro-luc (A) or IFN3pro-luc (B) in 24-well plates. At 16 h post-transfection, the cells were stimulated with PBS (control) or plasmids and infected with GCRV for 16 h or uninfected. Dual luciferase reporter assays were conducted at 12 h after GCRV infection. Treatment durations in this assay was far shorter than those in <t>qRT-PCR</t> which caused different results. Error bars indicate standard deviation (n = 4). Asterisks indicate significant difference from control (*, P ≤ 0.05).
Qrt Pcr Roche Lightcycler 480 System, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "A plasmid containing CpG ODN as vaccine adjuvant against grass carp reovirus in grass carp Ctenopharyngodon idella"

Article Title: A plasmid containing CpG ODN as vaccine adjuvant against grass carp reovirus in grass carp Ctenopharyngodon idella

Journal: Oncotarget

doi: 10.18632/oncotarget.21245

Immune responses of CiIFN1/3 to plasmids containing CpG ODNs Left, the mRNA expressions of CiIFN1 (A) and CiIFN3 (B) were measured at 24 and 48 h post-infection. CIK cells were stimulated with PBS (control) or plasmids containing CpG ODNs and infected with GCRV. Other captions were the same as Figure 3 . Right, CIK cells were co-transfected with 800 ng of pRL-TK and IFN1pro-luc (A) or IFN3pro-luc (B) in 24-well plates. At 16 h post-transfection, the cells were stimulated with PBS (control) or plasmids and infected with GCRV for 16 h or uninfected. Dual luciferase reporter assays were conducted at 12 h after GCRV infection. Treatment durations in this assay was far shorter than those in qRT-PCR which caused different results. Error bars indicate standard deviation (n = 4). Asterisks indicate significant difference from control (*, P ≤ 0.05).
Figure Legend Snippet: Immune responses of CiIFN1/3 to plasmids containing CpG ODNs Left, the mRNA expressions of CiIFN1 (A) and CiIFN3 (B) were measured at 24 and 48 h post-infection. CIK cells were stimulated with PBS (control) or plasmids containing CpG ODNs and infected with GCRV. Other captions were the same as Figure 3 . Right, CIK cells were co-transfected with 800 ng of pRL-TK and IFN1pro-luc (A) or IFN3pro-luc (B) in 24-well plates. At 16 h post-transfection, the cells were stimulated with PBS (control) or plasmids and infected with GCRV for 16 h or uninfected. Dual luciferase reporter assays were conducted at 12 h after GCRV infection. Treatment durations in this assay was far shorter than those in qRT-PCR which caused different results. Error bars indicate standard deviation (n = 4). Asterisks indicate significant difference from control (*, P ≤ 0.05).

Techniques Used: Infection, Transfection, Luciferase, Quantitative RT-PCR, Standard Deviation

Related Articles

SYBR Green Assay:

Article Title: A plasmid containing CpG ODN as vaccine adjuvant against grass carp reovirus in grass carp Ctenopharyngodon idella
Article Snippet: .. qRT-PCR Roche LightCycler® 480 system was used to quantify the mRNA expression of the listed genes followed by their GenBank accession number: CiTLR9 (FJ969850), CiRIG-I (GQ478334), CiIFN1 (DQ357216), CiIFN3 (KU182642), CiIFNγ2 (AGQ16237), CiIL-2 (AF486820), CiIL-12 (KF944668), NFκB1 (nuclear factor-kappaB 1) (KY613788), NFκB2 (KY613789), VP4 (GQ469997), CiIgM (DQ417927), CiIgD (GQ429174), CiIgZ (GQ201421), CiTNF-α (HQ696609), CiMx2 (JF699168) and endogenous reference (EF1α, GQ266394 and 18S rRNA, EU047719) using BioEasy Master Mix (SYBR Green) (Hangzhou Bioer Technology Co., Ltd, China). .. Melting curve analysis of amplification products was performed to confirm that only one PCR product was amplified and detected.

Polymerase Chain Reaction:

Article Title: A plasmid containing CpG ODN as vaccine adjuvant against grass carp reovirus in grass carp Ctenopharyngodon idella
Article Snippet: qRT-PCR Roche LightCycler® 480 system was used to quantify the mRNA expression of the listed genes followed by their GenBank accession number: CiTLR9 (FJ969850), CiRIG-I (GQ478334), CiIFN1 (DQ357216), CiIFN3 (KU182642), CiIFNγ2 (AGQ16237), CiIL-2 (AF486820), CiIL-12 (KF944668), NFκB1 (nuclear factor-kappaB 1) (KY613788), NFκB2 (KY613789), VP4 (GQ469997), CiIgM (DQ417927), CiIgD (GQ429174), CiIgZ (GQ201421), CiTNF-α (HQ696609), CiMx2 (JF699168) and endogenous reference (EF1α, GQ266394 and 18S rRNA, EU047719) using BioEasy Master Mix (SYBR Green) (Hangzhou Bioer Technology Co., Ltd, China). .. Melting curve analysis of amplification products was performed to confirm that only one PCR product was amplified and detected.

Amplification:

Article Title: A plasmid containing CpG ODN as vaccine adjuvant against grass carp reovirus in grass carp Ctenopharyngodon idella
Article Snippet: qRT-PCR Roche LightCycler® 480 system was used to quantify the mRNA expression of the listed genes followed by their GenBank accession number: CiTLR9 (FJ969850), CiRIG-I (GQ478334), CiIFN1 (DQ357216), CiIFN3 (KU182642), CiIFNγ2 (AGQ16237), CiIL-2 (AF486820), CiIL-12 (KF944668), NFκB1 (nuclear factor-kappaB 1) (KY613788), NFκB2 (KY613789), VP4 (GQ469997), CiIgM (DQ417927), CiIgD (GQ429174), CiIgZ (GQ201421), CiTNF-α (HQ696609), CiMx2 (JF699168) and endogenous reference (EF1α, GQ266394 and 18S rRNA, EU047719) using BioEasy Master Mix (SYBR Green) (Hangzhou Bioer Technology Co., Ltd, China). .. Melting curve analysis of amplification products was performed to confirm that only one PCR product was amplified and detected.

Quantitative RT-PCR:

Article Title: A plasmid containing CpG ODN as vaccine adjuvant against grass carp reovirus in grass carp Ctenopharyngodon idella
Article Snippet: .. qRT-PCR Roche LightCycler® 480 system was used to quantify the mRNA expression of the listed genes followed by their GenBank accession number: CiTLR9 (FJ969850), CiRIG-I (GQ478334), CiIFN1 (DQ357216), CiIFN3 (KU182642), CiIFNγ2 (AGQ16237), CiIL-2 (AF486820), CiIL-12 (KF944668), NFκB1 (nuclear factor-kappaB 1) (KY613788), NFκB2 (KY613789), VP4 (GQ469997), CiIgM (DQ417927), CiIgD (GQ429174), CiIgZ (GQ201421), CiTNF-α (HQ696609), CiMx2 (JF699168) and endogenous reference (EF1α, GQ266394 and 18S rRNA, EU047719) using BioEasy Master Mix (SYBR Green) (Hangzhou Bioer Technology Co., Ltd, China). .. Melting curve analysis of amplification products was performed to confirm that only one PCR product was amplified and detected.

Expressing:

Article Title: A plasmid containing CpG ODN as vaccine adjuvant against grass carp reovirus in grass carp Ctenopharyngodon idella
Article Snippet: .. qRT-PCR Roche LightCycler® 480 system was used to quantify the mRNA expression of the listed genes followed by their GenBank accession number: CiTLR9 (FJ969850), CiRIG-I (GQ478334), CiIFN1 (DQ357216), CiIFN3 (KU182642), CiIFNγ2 (AGQ16237), CiIL-2 (AF486820), CiIL-12 (KF944668), NFκB1 (nuclear factor-kappaB 1) (KY613788), NFκB2 (KY613789), VP4 (GQ469997), CiIgM (DQ417927), CiIgD (GQ429174), CiIgZ (GQ201421), CiTNF-α (HQ696609), CiMx2 (JF699168) and endogenous reference (EF1α, GQ266394 and 18S rRNA, EU047719) using BioEasy Master Mix (SYBR Green) (Hangzhou Bioer Technology Co., Ltd, China). .. Melting curve analysis of amplification products was performed to confirm that only one PCR product was amplified and detected.

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  • 95
    Roche lightcycler 480 real time qrt pcr instrument
    ZNF554 down-regulation in the villous trophoblast (VT) in preeclampsia. (A) Tissue <t>qRT-PCR</t> array revealed the highest ZNF554 expression in the placenta among 48 human tissues. Color code depicts gene expression levels relative to that of the placenta (100%). (B) In situ hybridization of a third trimester control placenta (GW29) and (C) immunohistochemistry of a first trimester placenta (GW12) shows mainly syncytiotrophoblastic ZNF554 expression (hematoxylin counterstaining, 1,400 and 400× magnifications, respectively). Black or white arrowheads depict syncytiotrophoblast or cytotrophoblast, while black arrow depicts fetal endothelium, respectively. (D) qRT-PCR revealed that ZNF554 expression is up-regulated during VT differentiation in parallel with CSH1 . (E,F) ZNF554 immunopositivity was faint in the syncytiotrophoblast in preeclampsia [ (F) , GW35] compared to gestational-age matched controls [ (E) , GW36] (hematoxylin counterstaining, 400× magnifications). Arrow and arrowhead depict syncytiotrophoblast and villous endothelium, respectively. (G) ZNF554 mRNA expression was 74% lower in ZNF554 -silenced BeWo cells compared to controls used for the microarrays ( p = 5.24 × 10 −6 ). (H) Nuclear and cytoplasmic ZNF554 immunofluorescence decreased in BeWo cells treated with ZNF554 siRNA compared to control cells (3,500× magnifications). (I) Molecular functions and one Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway (glycolysis/gluconeogenesis) affected in ZNF554 -silenced BeWo cells. Colors denote the proportions of up- or down-regulated genes (red: > 0.5 up-regulated; blue: > 0.5 down-regulated; black: 0.5–0.5 up- and down-regulated). Letter sizes represent the minus log 10 of p -values of the given functions or pathway. (J) qRT-PCR validated FSTL3 up-regulation (2.7-fold, p
    Lightcycler 480 Real Time Qrt Pcr Instrument, supplied by Roche, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Expression of NF-κB1 and Bcl-2 detection in glioma tissues by <t>qRT-PCR.</t> Notes: The expression of NF-κB1 in glioma was significantly higher than that in nonneoplastic brain tissues ( P
    Sybr Green Quantitative Real Time Polymerase Chain Reaction Qrt Pcr, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche lightcycler 480 qrt pcr system
    Activity of the JH I geometric isomers in vivo . A , capacity of the native S -( E , E )-JH I, its stereoisomers, and deoxy-JH I to induce ectopic expression of Kr-h1 mRNA in Drosophila pupae. Animals were treated at the white puparium stage and collected 24 h later. Shown are normalized mean <t>qRT-PCR</t> data from four biological replicates, each comprising three individual pupae for each compound. The mRNA levels are plotted on a logarithmic scale as -fold increase relative to treatment with solvent (acetone) alone for which the value was arbitrarily set to 1. Error bars represent S.D. Different letters above the data indicate that activity of the individual compounds differed significantly ( p
    Lightcycler 480 Qrt Pcr System, supplied by Roche, used in various techniques. Bioz Stars score: 95/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 qrt pcr system/product/Roche
    Average 95 stars, based on 4 article reviews
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    Carnosine suppresses the Aβ1-42-induced mRNA expression levels of iNOS, Nox1, and Nox2 and increases the expression of TGF-β1 mRNA. Effects of Aβ1-42 and carnosine (Aβ1-42 + Car (co-treat.) and Aβ1-42 + Car (co-inc.)) on ( A ) iNOS, ( B ) Nox1, ( C ) Nox2, and ( D ) TGF-β1 mRNAs expression were examined by <t>qRT-PCR.</t> The abundance of each mRNA of interest was expressed relative to the abundance of GAPDH-mRNA, as an internal control. As a negative control, a reaction in the absence of cDNA (no template control, NTC) was performed. qRT-PCR amplifications were performed in quadruplicate. Standard deviations are represented by vertical bars. * significantly different from resting cells, p
    Qrt Pcr Analysis, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 226 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ZNF554 down-regulation in the villous trophoblast (VT) in preeclampsia. (A) Tissue qRT-PCR array revealed the highest ZNF554 expression in the placenta among 48 human tissues. Color code depicts gene expression levels relative to that of the placenta (100%). (B) In situ hybridization of a third trimester control placenta (GW29) and (C) immunohistochemistry of a first trimester placenta (GW12) shows mainly syncytiotrophoblastic ZNF554 expression (hematoxylin counterstaining, 1,400 and 400× magnifications, respectively). Black or white arrowheads depict syncytiotrophoblast or cytotrophoblast, while black arrow depicts fetal endothelium, respectively. (D) qRT-PCR revealed that ZNF554 expression is up-regulated during VT differentiation in parallel with CSH1 . (E,F) ZNF554 immunopositivity was faint in the syncytiotrophoblast in preeclampsia [ (F) , GW35] compared to gestational-age matched controls [ (E) , GW36] (hematoxylin counterstaining, 400× magnifications). Arrow and arrowhead depict syncytiotrophoblast and villous endothelium, respectively. (G) ZNF554 mRNA expression was 74% lower in ZNF554 -silenced BeWo cells compared to controls used for the microarrays ( p = 5.24 × 10 −6 ). (H) Nuclear and cytoplasmic ZNF554 immunofluorescence decreased in BeWo cells treated with ZNF554 siRNA compared to control cells (3,500× magnifications). (I) Molecular functions and one Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway (glycolysis/gluconeogenesis) affected in ZNF554 -silenced BeWo cells. Colors denote the proportions of up- or down-regulated genes (red: > 0.5 up-regulated; blue: > 0.5 down-regulated; black: 0.5–0.5 up- and down-regulated). Letter sizes represent the minus log 10 of p -values of the given functions or pathway. (J) qRT-PCR validated FSTL3 up-regulation (2.7-fold, p

    Journal: Frontiers in Immunology

    Article Title: Integrated Systems Biology Approach Identifies Novel Maternal and Placental Pathways of Preeclampsia

    doi: 10.3389/fimmu.2018.01661

    Figure Lengend Snippet: ZNF554 down-regulation in the villous trophoblast (VT) in preeclampsia. (A) Tissue qRT-PCR array revealed the highest ZNF554 expression in the placenta among 48 human tissues. Color code depicts gene expression levels relative to that of the placenta (100%). (B) In situ hybridization of a third trimester control placenta (GW29) and (C) immunohistochemistry of a first trimester placenta (GW12) shows mainly syncytiotrophoblastic ZNF554 expression (hematoxylin counterstaining, 1,400 and 400× magnifications, respectively). Black or white arrowheads depict syncytiotrophoblast or cytotrophoblast, while black arrow depicts fetal endothelium, respectively. (D) qRT-PCR revealed that ZNF554 expression is up-regulated during VT differentiation in parallel with CSH1 . (E,F) ZNF554 immunopositivity was faint in the syncytiotrophoblast in preeclampsia [ (F) , GW35] compared to gestational-age matched controls [ (E) , GW36] (hematoxylin counterstaining, 400× magnifications). Arrow and arrowhead depict syncytiotrophoblast and villous endothelium, respectively. (G) ZNF554 mRNA expression was 74% lower in ZNF554 -silenced BeWo cells compared to controls used for the microarrays ( p = 5.24 × 10 −6 ). (H) Nuclear and cytoplasmic ZNF554 immunofluorescence decreased in BeWo cells treated with ZNF554 siRNA compared to control cells (3,500× magnifications). (I) Molecular functions and one Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway (glycolysis/gluconeogenesis) affected in ZNF554 -silenced BeWo cells. Colors denote the proportions of up- or down-regulated genes (red: > 0.5 up-regulated; blue: > 0.5 down-regulated; black: 0.5–0.5 up- and down-regulated). Letter sizes represent the minus log 10 of p -values of the given functions or pathway. (J) qRT-PCR validated FSTL3 up-regulation (2.7-fold, p

    Article Snippet: Quality control criteria included robust and specific amplification (Cp values < 40 cycles and CV < 10% for duplicates) of the bisulfite primers on a LightCycler 480 real-time qRT-PCR instrument (Roche Diagnostics Corp. Indianapolis, IN, USA).

    Techniques: Quantitative RT-PCR, Expressing, In Situ Hybridization, Immunohistochemistry, Immunofluorescence

    Gene modules associated with blood pressure (BP) and birthweight (BW). (A) Hierarchical clustering of qRT-PCR data obtained with 100 samples and 47 genes. Pearson correlation was used for similarity analysis and average method for linkage calculation. Samples were colored according to patient groups and maturity status. M1 (green) and M2 (red) module genes and 34 of 60 samples from women with preeclampsia clustered together. (B) Association of gene expression with BP and BW. The significance p -values for these coefficients were plotted for all genes, colored according to module classification (black: not changed on the microarray). Filled circles represent predominantly placenta-expressed genes and dashed lines the significance threshold at p = 0.05. Seven of 9 genes related to BW belong to module M1, while 10 of 15 genes related to BP are from module M2. Gene expression (C) and protein immunostaining (D) in Supplementary Material. (E) Representative images of the same placenta from a preterm control (left, 29 weeks) and a patient with preterm preeclampsia associated with SGA (right, 31 weeks) are shown for the immunostainings (hematoxylin counterstaining, 40× magnification).

    Journal: Frontiers in Immunology

    Article Title: Integrated Systems Biology Approach Identifies Novel Maternal and Placental Pathways of Preeclampsia

    doi: 10.3389/fimmu.2018.01661

    Figure Lengend Snippet: Gene modules associated with blood pressure (BP) and birthweight (BW). (A) Hierarchical clustering of qRT-PCR data obtained with 100 samples and 47 genes. Pearson correlation was used for similarity analysis and average method for linkage calculation. Samples were colored according to patient groups and maturity status. M1 (green) and M2 (red) module genes and 34 of 60 samples from women with preeclampsia clustered together. (B) Association of gene expression with BP and BW. The significance p -values for these coefficients were plotted for all genes, colored according to module classification (black: not changed on the microarray). Filled circles represent predominantly placenta-expressed genes and dashed lines the significance threshold at p = 0.05. Seven of 9 genes related to BW belong to module M1, while 10 of 15 genes related to BP are from module M2. Gene expression (C) and protein immunostaining (D) in Supplementary Material. (E) Representative images of the same placenta from a preterm control (left, 29 weeks) and a patient with preterm preeclampsia associated with SGA (right, 31 weeks) are shown for the immunostainings (hematoxylin counterstaining, 40× magnification).

    Article Snippet: Quality control criteria included robust and specific amplification (Cp values < 40 cycles and CV < 10% for duplicates) of the bisulfite primers on a LightCycler 480 real-time qRT-PCR instrument (Roche Diagnostics Corp. Indianapolis, IN, USA).

    Techniques: Quantitative RT-PCR, Expressing, Microarray, Immunostaining

    Expression of NF-κB1 and Bcl-2 detection in glioma tissues by qRT-PCR. Notes: The expression of NF-κB1 in glioma was significantly higher than that in nonneoplastic brain tissues ( P

    Journal: OncoTargets and therapy

    Article Title: Nuclear factor-kappa B1 inhibits early apoptosis of glioma cells by promoting the expression of Bcl-2

    doi: 10.2147/OTT.S144014

    Figure Lengend Snippet: Expression of NF-κB1 and Bcl-2 detection in glioma tissues by qRT-PCR. Notes: The expression of NF-κB1 in glioma was significantly higher than that in nonneoplastic brain tissues ( P

    Article Snippet: Relative levels of NF-κB1 and Bcl-2 mRNA were examined using SYBR green quantitative real-time polymerase chain reaction (qRT-PCR) (LightCycler® 480; Hoffman-La Roche Ltd., Basel, Switzerland) and were normalized to levels of GAPDH mRNA.

    Techniques: Expressing, Quantitative RT-PCR

    Activity of the JH I geometric isomers in vivo . A , capacity of the native S -( E , E )-JH I, its stereoisomers, and deoxy-JH I to induce ectopic expression of Kr-h1 mRNA in Drosophila pupae. Animals were treated at the white puparium stage and collected 24 h later. Shown are normalized mean qRT-PCR data from four biological replicates, each comprising three individual pupae for each compound. The mRNA levels are plotted on a logarithmic scale as -fold increase relative to treatment with solvent (acetone) alone for which the value was arbitrarily set to 1. Error bars represent S.D. Different letters above the data indicate that activity of the individual compounds differed significantly ( p

    Journal: The Journal of Biological Chemistry

    Article Title: Exquisite ligand stereoselectivity of a Drosophila juvenile hormone receptor contrasts with its broad agonist repertoire

    doi: 10.1074/jbc.RA118.005992

    Figure Lengend Snippet: Activity of the JH I geometric isomers in vivo . A , capacity of the native S -( E , E )-JH I, its stereoisomers, and deoxy-JH I to induce ectopic expression of Kr-h1 mRNA in Drosophila pupae. Animals were treated at the white puparium stage and collected 24 h later. Shown are normalized mean qRT-PCR data from four biological replicates, each comprising three individual pupae for each compound. The mRNA levels are plotted on a logarithmic scale as -fold increase relative to treatment with solvent (acetone) alone for which the value was arbitrarily set to 1. Error bars represent S.D. Different letters above the data indicate that activity of the individual compounds differed significantly ( p

    Article Snippet: Transcripts were quantified using a LightCycler 480 qRT-PCR System (Roche Applied Science) with SYBR Green fluorescent label and previously described primers specific for Kr-h1 and the ribosomal protein 49 ( rp49 ) genes ( ).

    Techniques: Activity Assay, In Vivo, Expressing, Quantitative RT-PCR

    Carnosine suppresses the Aβ1-42-induced mRNA expression levels of iNOS, Nox1, and Nox2 and increases the expression of TGF-β1 mRNA. Effects of Aβ1-42 and carnosine (Aβ1-42 + Car (co-treat.) and Aβ1-42 + Car (co-inc.)) on ( A ) iNOS, ( B ) Nox1, ( C ) Nox2, and ( D ) TGF-β1 mRNAs expression were examined by qRT-PCR. The abundance of each mRNA of interest was expressed relative to the abundance of GAPDH-mRNA, as an internal control. As a negative control, a reaction in the absence of cDNA (no template control, NTC) was performed. qRT-PCR amplifications were performed in quadruplicate. Standard deviations are represented by vertical bars. * significantly different from resting cells, p

    Journal: Cells

    Article Title: Carnosine Prevents Aβ-Induced Oxidative Stress and Inflammation in Microglial Cells: A Key Role of TGF-β1

    doi: 10.3390/cells8010064

    Figure Lengend Snippet: Carnosine suppresses the Aβ1-42-induced mRNA expression levels of iNOS, Nox1, and Nox2 and increases the expression of TGF-β1 mRNA. Effects of Aβ1-42 and carnosine (Aβ1-42 + Car (co-treat.) and Aβ1-42 + Car (co-inc.)) on ( A ) iNOS, ( B ) Nox1, ( C ) Nox2, and ( D ) TGF-β1 mRNAs expression were examined by qRT-PCR. The abundance of each mRNA of interest was expressed relative to the abundance of GAPDH-mRNA, as an internal control. As a negative control, a reaction in the absence of cDNA (no template control, NTC) was performed. qRT-PCR amplifications were performed in quadruplicate. Standard deviations are represented by vertical bars. * significantly different from resting cells, p

    Article Snippet: Next, each sample was quantified, diluted to a final concentration of 25 ng/µL, and used for qRT-PCR analysis (LightCycler® 480 System, Roche Molecular Systems, Inc., Pleasanton, CA, USA).

    Techniques: Expressing, Quantitative RT-PCR, Negative Control