qiashredder  (Qiagen)


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  • 99
    Name:
    QIAshredder homogenizer
    Description:

    Catalog Number:
    79654
    Price:
    None
    Score:
    85
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    Structured Review

    Qiagen qiashredder

    https://www.bioz.com/result/qiashredder/product/Qiagen
    Average 99 stars, based on 578 article reviews
    Price from $9.99 to $1999.99
    qiashredder - by Bioz Stars, 2019-12
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Evidence that CED-9/Bcl2 and CED-4/Apaf-1 localization is not consistent with the current model for C. elegans apoptosis induction
    Article Snippet: To further lyse the sample and to clear the lysate from non-lysed worms and worm cuticles, the sample was passed through cell-lysate homogenizer tubes (Qiashredder, Qiagen) and centrifuged for 2 min at 100 × r.c.f. (Eppendorf cooling benchtop centrifuge). .. To further lyse the sample and to clear the lysate from non-lysed worms and worm cuticles, the sample was passed through cell-lysate homogenizer tubes (Qiashredder, Qiagen) and centrifuged for 2 min at 100 × r.c.f. (Eppendorf cooling benchtop centrifuge).

    Article Title: The transcription factors Ik-1 and MZF1 downregulate IGF-IR expression in NPM-ALK+ T-cell lymphoma
    Article Snippet: Total RNA was isolated and purified using the RNAeasy Mini Kit (Qiagen). .. Briefly, 1 × 106 cells were collected by centrifugation at 200 g for 5 min, washed twice in phosphate-buffered saline (PBS), and subjected to lysis and homogenization with Buffer RLT using QiaShredder spin columns (Qiagen). .. Homogenized cells were re-suspended in an equal volume of 70% ethanol and passed through the spin columns.

    Amplification:

    Article Title: Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat
    Article Snippet: Total RNA was isolated from spleen, thymus, bone marrow and lymph nodes (mesenteric and cervical) from wild type LEW/Ztm rats, heterozygous and homozygous Rag1 mutant rats (n=10) using the RNeasy Mini Kit (Qiagen, Hilden, Germany) after homogenization of the tissues with the UP100H ultrasonic device (Hielscher, Teltow, Germany) and Qiashredder (Qiagen, Hilden, Germany). .. Total RNA was isolated from spleen, thymus, bone marrow and lymph nodes (mesenteric and cervical) from wild type LEW/Ztm rats, heterozygous and homozygous Rag1 mutant rats (n=10) using the RNeasy Mini Kit (Qiagen, Hilden, Germany) after homogenization of the tissues with the UP100H ultrasonic device (Hielscher, Teltow, Germany) and Qiashredder (Qiagen, Hilden, Germany).

    High Throughput Screening Assay:

    Article Title: A miRNA upregulated in asthma airway T cells promotes TH2 cytokine production
    Article Snippet: Paragraph title: High-throughput multiplex qPCR ... Frozen lysates were thawed and loaded onto QIAshredder homogenizer columns (Qiagen).

    Positive Control:

    Article Title: Evaluation of cell damage induced by irradiated Zinc-Phthalocyanine-gold dendrimeric nanoparticles in a breast cancer cell line
    Article Snippet: An apoptosis positive control was prepared by inducing apoptosis with 1 μg/ml actinomycin D, and a necrosis positive control with 10% (v/v) hydrogen peroxide for 24 h. The real-time reverse transcriptase polymerase chain reaction (RT-PCR) was used to evaluate the expression of 84 genes following treatment (3 h incubation). .. Total RNA from untreated, MPDC-treated and PDT-treated cells was isolated using the RNeasy kit (Qiagen, 74104) with QIA shredder homogenizers (Qiagen, 79654).

    Cytometry:

    Article Title: Evaluation of cell damage induced by irradiated Zinc-Phthalocyanine-gold dendrimeric nanoparticles in a breast cancer cell line
    Article Snippet: Then 400 μl of 1 × binding buffer was added to all the samples which were analyzed on the FACSCAria flow cytometer (BD Biosciences) by reading 10 000 events. .. Total RNA from untreated, MPDC-treated and PDT-treated cells was isolated using the RNeasy kit (Qiagen, 74104) with QIA shredder homogenizers (Qiagen, 79654).

    Article Title: An RNA Alternative to Human Transferrin: A New Tool for Targeting Human Cells
    Article Snippet: Half of the cells in each sample were centrifuged at 300g for 3 minutes and resuspended in flow cytometry buffer consisting of HBSS with 1% bovine serum albumin and 0.1% sodium azide. .. The second half of each sample was passed through a QIAshredder cell homogenizer (QIAGEN).

    Article Title: A miRNA upregulated in asthma airway T cells promotes TH2 cytokine production
    Article Snippet: Briefly, CD4 T cells sorted by flow cytometry from clinical samples were lysed and stored in RLT buffer (Qiagen) at -80°C until all samples were collected. .. Frozen lysates were thawed and loaded onto QIAshredder homogenizer columns (Qiagen).

    Quantitative RT-PCR:

    Article Title: Transmitted/Founder Hepatitis C Viruses Induce Cell-Type- and Genotype-Specific Differences in Innate Signaling within the Liver
    Article Snippet: At 8 h posttransfection, cultures were lysed for gene expression analysis using RLT lysis buffer (Qiagen, Valencia, CA) with 10 µl β-mercaptoethanol added per ml of lysis buffer to stabilize RNA. .. The RNA was sent through QiaShredder homogenizing columns (Qiagen) and then stored at −80°C prior to further analysis using qRT-PCR. .. Human PBMCs were maintained in medium containing RPMI plus 10% human serum.

    Article Title: Nanocomposite Scaffold for Chondrocyte Growth and Cartilage Tissue Engineering: Effects of Carbon Nanotube Surface Functionalization
    Article Snippet: Gene expression of ECM genes (aggrecan, collagen-1, collagen-2, and collagen-10), surface adhesion proteins (fibronectin, CD44), and tumor marker (p53) levels was determined by quantitative RT-PCR from chondrocytes cultured in hydrogel nanocomposite scaffolds. .. Homogenization was performed with QIAShredder (Qiagen) according to the manufacturer's protocol.

    Article Title: Effects of Chronic Exposure to an Environmentally Relevant Mixture of Brominated Flame Retardants on the Reproductive and Thyroid System in Adult Male Rats
    Article Snippet: Frozen left testes (n = 9–10 per group) were disrupted using a mortar and pestle followed by homogenization via a 20 gauge needle and syringe combined with QIAshredder (Qiagen Inc.). .. Frozen left testes (n = 9–10 per group) were disrupted using a mortar and pestle followed by homogenization via a 20 gauge needle and syringe combined with QIAshredder (Qiagen Inc.).

    SYBR Green Assay:

    Article Title: Chicken and Duck Myotubes Are Highly Susceptible and Permissive to Influenza Virus Infection
    Article Snippet: Cells were homogenized with a QIAshredder homogenizer (Qiagen), followed by the removal of genomic DNA (gDNA) using a gDNA eliminator column. .. Cells were homogenized with a QIAshredder homogenizer (Qiagen), followed by the removal of genomic DNA (gDNA) using a gDNA eliminator column.

    Article Title: Effects of Chronic Exposure to an Environmentally Relevant Mixture of Brominated Flame Retardants on the Reproductive and Thyroid System in Adult Male Rats
    Article Snippet: Frozen left testes (n = 9–10 per group) were disrupted using a mortar and pestle followed by homogenization via a 20 gauge needle and syringe combined with QIAshredder (Qiagen Inc.). .. Frozen left testes (n = 9–10 per group) were disrupted using a mortar and pestle followed by homogenization via a 20 gauge needle and syringe combined with QIAshredder (Qiagen Inc.).

    Article Title: Comparison of Pre- and Post-translational Expressions of COXIV-1 and MT-ATPase 6 Genes in Colorectal Adenoma-Carcinoma Tissues
    Article Snippet: Following tissue homogenization, samples were placed in QIAshredder (Qiagen, Germantown, MD, US) columns to reduce viscosity. .. Reverse transcription (RT) PCR was performed with iscript cDNA synthesis kits (BioRad, Hercules, CA, US) following the manufacturer’s instructions, with 500 ng of total RNA as a template and random hexamers as primers.

    Article Title: Fibroblast-specific TGF- β–Smad2/3 signaling underlies cardiac fibrosis
    Article Snippet: RNA was isolated from fibroblasts using QIAshredder homogenization and the RNAeasy Kit according to the manufacturer’s instructions (QIAGEN, catalog 74104). .. RNA was isolated from fibroblasts using QIAshredder homogenization and the RNAeasy Kit according to the manufacturer’s instructions (QIAGEN, catalog 74104).

    Article Title: The secreted protein S100A7 (psoriasin) is induced by telomere dysfunction in human keratinocytes independently of a DNA damage response and cell cycle regulators
    Article Snippet: Extraction of total RNA was performed using the RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions, including sample homogenisation with QIAshredder (Qiagen) and DNase treatment with the RNase-free DNase Set (Qiagen). .. Purified RNA was reverse transcribed using with Finnzymes DyNAmo™ cDNA Synthesis Kit (New England BioLabs, Hitchin, UK) and the cDNA stored at -20°C.

    Random Hexamer Labeling:

    Article Title: An RNA Alternative to Human Transferrin: A New Tool for Targeting Human Cells
    Article Snippet: The second half of each sample was passed through a QIAshredder cell homogenizer (QIAGEN). .. Total cell RNA for each sample was isolated from the cell lysate according to QIAGEN's RNAeasy Plus Mini Kit protocol.

    Luciferase:

    Article Title: Microscopic lymph node tumor burden quantified by macroscopic dual-tracer molecular imaging
    Article Snippet: Since a human breast cancer cell line was grown in a mouse model in this study, it was possible to use genetic markers to determine the ratio of cancer to normal tissue with real-time PCR based on established approaches., For improved sensitivity in this regard, the presence of luciferase gene expression was analyzed instead of a human specific gene, since the luciferase gene in not expressed naturally by mice but was transfected into the MDA-MB-231-luc-D3H2LN cell line by the manufacturer. .. Total RNA was isolated from flash frozen lymph nodes using RNeasy mini kit and Qiashredder (Qiagen, Venlo, Netherlands), and 18-gauge needles to homogenize the tissue.

    Infection:

    Article Title: Transmitted/Founder Hepatitis C Viruses Induce Cell-Type- and Genotype-Specific Differences in Innate Signaling within the Liver
    Article Snippet: The RNA was sent through QiaShredder homogenizing columns (Qiagen) and then stored at −80°C prior to further analysis using qRT-PCR. .. The RNA was sent through QiaShredder homogenizing columns (Qiagen) and then stored at −80°C prior to further analysis using qRT-PCR.

    Expressing:

    Article Title: Evaluation of cell damage induced by irradiated Zinc-Phthalocyanine-gold dendrimeric nanoparticles in a breast cancer cell line
    Article Snippet: An apoptosis positive control was prepared by inducing apoptosis with 1 μg/ml actinomycin D, and a necrosis positive control with 10% (v/v) hydrogen peroxide for 24 h. The real-time reverse transcriptase polymerase chain reaction (RT-PCR) was used to evaluate the expression of 84 genes following treatment (3 h incubation). .. Total RNA from untreated, MPDC-treated and PDT-treated cells was isolated using the RNeasy kit (Qiagen, 74104) with QIA shredder homogenizers (Qiagen, 79654).

    Article Title: An RNA Alternative to Human Transferrin: A New Tool for Targeting Human Cells
    Article Snippet: These samples were analyzed for knockdown of GFP expression and uptake of Cy5-siRNA on a Sony iCyt Eclipse Analyzer. .. The second half of each sample was passed through a QIAshredder cell homogenizer (QIAGEN).

    Article Title: Transmitted/Founder Hepatitis C Viruses Induce Cell-Type- and Genotype-Specific Differences in Innate Signaling within the Liver
    Article Snippet: At 8 h posttransfection, cultures were lysed for gene expression analysis using RLT lysis buffer (Qiagen, Valencia, CA) with 10 µl β-mercaptoethanol added per ml of lysis buffer to stabilize RNA. .. The RNA was sent through QiaShredder homogenizing columns (Qiagen) and then stored at −80°C prior to further analysis using qRT-PCR.

    Article Title: Nanocomposite Scaffold for Chondrocyte Growth and Cartilage Tissue Engineering: Effects of Carbon Nanotube Surface Functionalization
    Article Snippet: Paragraph title: Gene expression ... Homogenization was performed with QIAShredder (Qiagen) according to the manufacturer's protocol.

    Article Title: Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat
    Article Snippet: Total RNA was isolated from spleen, thymus, bone marrow and lymph nodes (mesenteric and cervical) from wild type LEW/Ztm rats, heterozygous and homozygous Rag1 mutant rats (n=10) using the RNeasy Mini Kit (Qiagen, Hilden, Germany) after homogenization of the tissues with the UP100H ultrasonic device (Hielscher, Teltow, Germany) and Qiashredder (Qiagen, Hilden, Germany). .. Rat GAPDH was amplified with the following primers 5’-AGGGCTGCCTTCTCTTGTGAC-‘3 and 5’-CCGTGGGTAGAGTCATAC TGG-‘3 with an annealing temperature of 57°C for 25 cycles using the HotStarTaq Master Mix Kit (Qiagen, Hilden, Germany).

    Article Title: Microscopic lymph node tumor burden quantified by macroscopic dual-tracer molecular imaging
    Article Snippet: Since a human breast cancer cell line was grown in a mouse model in this study, it was possible to use genetic markers to determine the ratio of cancer to normal tissue with real-time PCR based on established approaches., For improved sensitivity in this regard, the presence of luciferase gene expression was analyzed instead of a human specific gene, since the luciferase gene in not expressed naturally by mice but was transfected into the MDA-MB-231-luc-D3H2LN cell line by the manufacturer. .. Total RNA was isolated from flash frozen lymph nodes using RNeasy mini kit and Qiashredder (Qiagen, Venlo, Netherlands), and 18-gauge needles to homogenize the tissue.

    Article Title: Fibroblast-specific TGF- β–Smad2/3 signaling underlies cardiac fibrosis
    Article Snippet: RNA was isolated from fibroblasts using QIAshredder homogenization and the RNAeasy Kit according to the manufacturer’s instructions (QIAGEN, catalog 74104). .. RNA was isolated from fibroblasts using QIAshredder homogenization and the RNAeasy Kit according to the manufacturer’s instructions (QIAGEN, catalog 74104).

    Article Title: The secreted protein S100A7 (psoriasin) is induced by telomere dysfunction in human keratinocytes independently of a DNA damage response and cell cycle regulators
    Article Snippet: Extraction of total RNA was performed using the RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions, including sample homogenisation with QIAshredder (Qiagen) and DNase treatment with the RNase-free DNase Set (Qiagen). .. RT quantitative real-time PCR (absolute copy number quantification using standard curve for each gene) was performed using SYBR® green I Master (hot-start Taq polymerase master mix) in the LightCycler 480® qPCR system (Roche Applied Science, Welwyn Garden City, Herts., UK).

    Western Blot:

    Article Title: The Transcriptional Complex Between the BCL2 i-Motif and hnRNP LL Is a Molecular Switch for Control of Gene Expression That Can Be Modulated by Small Molecules
    Article Snippet: After homogenization using QIAshredder (Qiagen), the extract was centrifuged at 14 00 rpm at 4 °C for 15 min. .. After homogenization using QIAshredder (Qiagen), the extract was centrifuged at 14 00 rpm at 4 °C for 15 min.

    Derivative Assay:

    Article Title: A miRNA upregulated in asthma airway T cells promotes TH2 cytokine production
    Article Snippet: Frozen lysates were thawed and loaded onto QIAshredder homogenizer columns (Qiagen). .. Frozen lysates were thawed and loaded onto QIAshredder homogenizer columns (Qiagen).

    Flow Cytometry:

    Article Title: Evaluation of cell damage induced by irradiated Zinc-Phthalocyanine-gold dendrimeric nanoparticles in a breast cancer cell line
    Article Snippet: Then 400 μl of 1 × binding buffer was added to all the samples which were analyzed on the FACSCAria flow cytometer (BD Biosciences) by reading 10 000 events. .. Total RNA from untreated, MPDC-treated and PDT-treated cells was isolated using the RNeasy kit (Qiagen, 74104) with QIA shredder homogenizers (Qiagen, 79654).

    Article Title: An RNA Alternative to Human Transferrin: A New Tool for Targeting Human Cells
    Article Snippet: Half of the cells in each sample were centrifuged at 300g for 3 minutes and resuspended in flow cytometry buffer consisting of HBSS with 1% bovine serum albumin and 0.1% sodium azide. .. The second half of each sample was passed through a QIAshredder cell homogenizer (QIAGEN).

    Article Title: A miRNA upregulated in asthma airway T cells promotes TH2 cytokine production
    Article Snippet: Briefly, CD4 T cells sorted by flow cytometry from clinical samples were lysed and stored in RLT buffer (Qiagen) at -80°C until all samples were collected. .. Frozen lysates were thawed and loaded onto QIAshredder homogenizer columns (Qiagen).

    Concentration Assay:

    Article Title: Evaluation of cell damage induced by irradiated Zinc-Phthalocyanine-gold dendrimeric nanoparticles in a breast cancer cell line
    Article Snippet: Total RNA from untreated, MPDC-treated and PDT-treated cells was isolated using the RNeasy kit (Qiagen, 74104) with QIA shredder homogenizers (Qiagen, 79654). .. Total RNA from untreated, MPDC-treated and PDT-treated cells was isolated using the RNeasy kit (Qiagen, 74104) with QIA shredder homogenizers (Qiagen, 79654).

    Article Title: Human non-small cell lung cancer expresses putative cancer stem cell markers and exhibits the transcriptomic profile of multipotent cells
    Article Snippet: Briefly, the cells were lysed with lysis buffer and homogenized using the QIAshredder Homogenizer (Qiagen). .. Briefly, the cells were lysed with lysis buffer and homogenized using the QIAshredder Homogenizer (Qiagen).

    Article Title: A miRNA upregulated in asthma airway T cells promotes TH2 cytokine production
    Article Snippet: Frozen lysates were thawed and loaded onto QIAshredder homogenizer columns (Qiagen). .. Using a low concentration of ethanol (35% v/v), the large fraction of RNA ( > 150nt) was precipitated and captured on collection columns (RNeasy, Qiagen).

    Transfection:

    Article Title: An RNA Alternative to Human Transferrin: A New Tool for Targeting Human Cells
    Article Snippet: Transfected cells were washed with PBS and trypsinized. .. The second half of each sample was passed through a QIAshredder cell homogenizer (QIAGEN).

    Article Title: Transmitted/Founder Hepatitis C Viruses Induce Cell-Type- and Genotype-Specific Differences in Innate Signaling within the Liver
    Article Snippet: A master mix for each transfection condition was made following manufacturer’s protocol (Trans IT-mRNA kit; Mirus, Madison, WI). .. The RNA was sent through QiaShredder homogenizing columns (Qiagen) and then stored at −80°C prior to further analysis using qRT-PCR.

    Article Title: Microscopic lymph node tumor burden quantified by macroscopic dual-tracer molecular imaging
    Article Snippet: Since a human breast cancer cell line was grown in a mouse model in this study, it was possible to use genetic markers to determine the ratio of cancer to normal tissue with real-time PCR based on established approaches., For improved sensitivity in this regard, the presence of luciferase gene expression was analyzed instead of a human specific gene, since the luciferase gene in not expressed naturally by mice but was transfected into the MDA-MB-231-luc-D3H2LN cell line by the manufacturer. .. Total RNA was isolated from flash frozen lymph nodes using RNeasy mini kit and Qiashredder (Qiagen, Venlo, Netherlands), and 18-gauge needles to homogenize the tissue.

    Protease Inhibitor:

    Article Title: Evidence that CED-9/Bcl2 and CED-4/Apaf-1 localization is not consistent with the current model for C. elegans apoptosis induction
    Article Snippet: Worms were resuspended in 0.2 ml ice-cold extraction buffer CE1 supplemented with protease inhibitor (Qiagen Qproteome Cell Compartment Kit, Qiagen, Hamburg, Germany). .. To further lyse the sample and to clear the lysate from non-lysed worms and worm cuticles, the sample was passed through cell-lysate homogenizer tubes (Qiashredder, Qiagen) and centrifuged for 2 min at 100 × r.c.f. (Eppendorf cooling benchtop centrifuge).

    Cell Culture:

    Article Title: Transmitted/Founder Hepatitis C Viruses Induce Cell-Type- and Genotype-Specific Differences in Innate Signaling within the Liver
    Article Snippet: Paragraph title: Cell culture. ... The RNA was sent through QiaShredder homogenizing columns (Qiagen) and then stored at −80°C prior to further analysis using qRT-PCR.

    Article Title: Nanocomposite Scaffold for Chondrocyte Growth and Cartilage Tissue Engineering: Effects of Carbon Nanotube Surface Functionalization
    Article Snippet: Gene expression of ECM genes (aggrecan, collagen-1, collagen-2, and collagen-10), surface adhesion proteins (fibronectin, CD44), and tumor marker (p53) levels was determined by quantitative RT-PCR from chondrocytes cultured in hydrogel nanocomposite scaffolds. .. Homogenization was performed with QIAShredder (Qiagen) according to the manufacturer's protocol.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Evaluation of cell damage induced by irradiated Zinc-Phthalocyanine-gold dendrimeric nanoparticles in a breast cancer cell line
    Article Snippet: An apoptosis positive control was prepared by inducing apoptosis with 1 μg/ml actinomycin D, and a necrosis positive control with 10% (v/v) hydrogen peroxide for 24 h. The real-time reverse transcriptase polymerase chain reaction (RT-PCR) was used to evaluate the expression of 84 genes following treatment (3 h incubation). .. Total RNA from untreated, MPDC-treated and PDT-treated cells was isolated using the RNeasy kit (Qiagen, 74104) with QIA shredder homogenizers (Qiagen, 79654).

    Article Title: Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat
    Article Snippet: Paragraph title: RNA and RT-PCR ... Total RNA was isolated from spleen, thymus, bone marrow and lymph nodes (mesenteric and cervical) from wild type LEW/Ztm rats, heterozygous and homozygous Rag1 mutant rats (n=10) using the RNeasy Mini Kit (Qiagen, Hilden, Germany) after homogenization of the tissues with the UP100H ultrasonic device (Hielscher, Teltow, Germany) and Qiashredder (Qiagen, Hilden, Germany).

    Article Title: Comparison of Pre- and Post-translational Expressions of COXIV-1 and MT-ATPase 6 Genes in Colorectal Adenoma-Carcinoma Tissues
    Article Snippet: Following tissue homogenization, samples were placed in QIAshredder (Qiagen, Germantown, MD, US) columns to reduce viscosity. .. Following tissue homogenization, samples were placed in QIAshredder (Qiagen, Germantown, MD, US) columns to reduce viscosity.

    Sedimentation:

    Article Title: Evidence that CED-9/Bcl2 and CED-4/Apaf-1 localization is not consistent with the current model for C. elegans apoptosis induction
    Article Snippet: Worms were washed 3 × in M9 buffer and collected by sedimentation. .. To further lyse the sample and to clear the lysate from non-lysed worms and worm cuticles, the sample was passed through cell-lysate homogenizer tubes (Qiashredder, Qiagen) and centrifuged for 2 min at 100 × r.c.f. (Eppendorf cooling benchtop centrifuge).

    other:

    Article Title: Male infertility in mice lacking the store-operated Ca2+ channel Orai1
    Article Snippet: Bouin’s fixative was purchased from Polysciences, Inc. QIAshredder Homogenizer Columns, RNeasy Plus Mini Kits and Omniscript RT Kits were purchased from Qiagen.

    Sequencing:

    Article Title: Chicken and Duck Myotubes Are Highly Susceptible and Permissive to Influenza Virus Infection
    Article Snippet: Cells were homogenized with a QIAshredder homogenizer (Qiagen), followed by the removal of genomic DNA (gDNA) using a gDNA eliminator column. .. Cells were homogenized with a QIAshredder homogenizer (Qiagen), followed by the removal of genomic DNA (gDNA) using a gDNA eliminator column.

    Fractionation:

    Article Title: Evidence that CED-9/Bcl2 and CED-4/Apaf-1 localization is not consistent with the current model for C. elegans apoptosis induction
    Article Snippet: To further lyse the sample and to clear the lysate from non-lysed worms and worm cuticles, the sample was passed through cell-lysate homogenizer tubes (Qiashredder, Qiagen) and centrifuged for 2 min at 100 × r.c.f. (Eppendorf cooling benchtop centrifuge). .. To further lyse the sample and to clear the lysate from non-lysed worms and worm cuticles, the sample was passed through cell-lysate homogenizer tubes (Qiashredder, Qiagen) and centrifuged for 2 min at 100 × r.c.f. (Eppendorf cooling benchtop centrifuge).

    Binding Assay:

    Article Title: Evaluation of cell damage induced by irradiated Zinc-Phthalocyanine-gold dendrimeric nanoparticles in a breast cancer cell line
    Article Snippet: Then 400 μl of 1 × binding buffer was added to all the samples which were analyzed on the FACSCAria flow cytometer (BD Biosciences) by reading 10 000 events. .. Total RNA from untreated, MPDC-treated and PDT-treated cells was isolated using the RNeasy kit (Qiagen, 74104) with QIA shredder homogenizers (Qiagen, 79654).

    Article Title: The Transcriptional Complex Between the BCL2 i-Motif and hnRNP LL Is a Molecular Switch for Control of Gene Expression That Can Be Modulated by Small Molecules
    Article Snippet: After homogenization using QIAshredder (Qiagen), the extract was centrifuged at 14 00 rpm at 4 °C for 15 min. .. As a negative control, ∼0.5–1 μg of IgG, optimized to adjust the heavy chain signal in IP samples of IgG and Sp1 by Western blot, was used.

    Article Title: The secreted protein S100A7 (psoriasin) is induced by telomere dysfunction in human keratinocytes independently of a DNA damage response and cell cycle regulators
    Article Snippet: Extraction of total RNA was performed using the RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions, including sample homogenisation with QIAshredder (Qiagen) and DNase treatment with the RNase-free DNase Set (Qiagen). .. Target gene relative expression levels were calculated in relation to keratinocyte reference genes POLR2A and YAP1 [ ] using the LightCycler 480® Relative Quantification Software (with built-in multiple reference genes normalisation algorithm).

    Nucleic Acid Electrophoresis:

    Article Title: Effects of Chronic Exposure to an Environmentally Relevant Mixture of Brominated Flame Retardants on the Reproductive and Thyroid System in Adult Male Rats
    Article Snippet: Frozen left testes (n = 9–10 per group) were disrupted using a mortar and pestle followed by homogenization via a 20 gauge needle and syringe combined with QIAshredder (Qiagen Inc.). .. RNA extraction was carried out using an RNeasy Plus Mini Kit with on-column DNase digestion (Qiagen Inc.) according to the manufacturer’s protocol.

    Mutagenesis:

    Article Title: Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat
    Article Snippet: After reaction clean up with the Qiaquick Gel Extraction Kit (Qiagen, Hilden, Germany) the PCR products containing the Rag1 mutation were cleaved by Sty I-HF (NEB, Frankfurt, Germany) into 603 bp and 449 bp, while the wild type Rag1 fragments remained unchanged. .. Total RNA was isolated from spleen, thymus, bone marrow and lymph nodes (mesenteric and cervical) from wild type LEW/Ztm rats, heterozygous and homozygous Rag1 mutant rats (n=10) using the RNeasy Mini Kit (Qiagen, Hilden, Germany) after homogenization of the tissues with the UP100H ultrasonic device (Hielscher, Teltow, Germany) and Qiashredder (Qiagen, Hilden, Germany). .. RNA concentrations were determined with the Nanodrop spectrophotometer (Peqlab, Erlangen, Germany).

    Isolation:

    Article Title: Evaluation of cell damage induced by irradiated Zinc-Phthalocyanine-gold dendrimeric nanoparticles in a breast cancer cell line
    Article Snippet: Cells were detached and rinsed with PBS to remove all traces of culture media. .. Total RNA from untreated, MPDC-treated and PDT-treated cells was isolated using the RNeasy kit (Qiagen, 74104) with QIA shredder homogenizers (Qiagen, 79654). .. Cells were re-suspended in 600 μl RLT buffer and loaded on the QIA cube (Invitrogen).

    Article Title: Human non-small cell lung cancer expresses putative cancer stem cell markers and exhibits the transcriptomic profile of multipotent cells
    Article Snippet: Total RNA was extracted from up to 1 × 106 CD166+ /CD44+ and CD166+ /EpCAM+ PHBEC, A549, and H2170 cells using the Qiagen AllPrep DNA/RNA Isolation Kit (Qiagen) according to the manufacturer’s protocol. .. Briefly, the cells were lysed with lysis buffer and homogenized using the QIAshredder Homogenizer (Qiagen).

    Article Title: Chicken and Duck Myotubes Are Highly Susceptible and Permissive to Influenza Virus Infection
    Article Snippet: Total RNA was isolated from cells grown in 12-well plates by using an RNeasy Plus minikit (Qiagen). .. Cells were homogenized with a QIAshredder homogenizer (Qiagen), followed by the removal of genomic DNA (gDNA) using a gDNA eliminator column.

    Article Title: An RNA Alternative to Human Transferrin: A New Tool for Targeting Human Cells
    Article Snippet: Flow cytometry and RNA isolation . .. The second half of each sample was passed through a QIAshredder cell homogenizer (QIAGEN).

    Article Title: Nanocomposite Scaffold for Chondrocyte Growth and Cartilage Tissue Engineering: Effects of Carbon Nanotube Surface Functionalization
    Article Snippet: Total RNA was isolated from hydrogel nanocomposite constructs using the RNEasy kit (Qiagen), where the lysis buffer was supplemented with β-mercaptoethanol (1%) and 2M sodium acetate (0.66%). .. Homogenization was performed with QIAShredder (Qiagen) according to the manufacturer's protocol.

    Article Title: Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat
    Article Snippet: After reaction clean up with the Qiaquick Gel Extraction Kit (Qiagen, Hilden, Germany) the PCR products containing the Rag1 mutation were cleaved by Sty I-HF (NEB, Frankfurt, Germany) into 603 bp and 449 bp, while the wild type Rag1 fragments remained unchanged. .. Total RNA was isolated from spleen, thymus, bone marrow and lymph nodes (mesenteric and cervical) from wild type LEW/Ztm rats, heterozygous and homozygous Rag1 mutant rats (n=10) using the RNeasy Mini Kit (Qiagen, Hilden, Germany) after homogenization of the tissues with the UP100H ultrasonic device (Hielscher, Teltow, Germany) and Qiashredder (Qiagen, Hilden, Germany). .. RNA concentrations were determined with the Nanodrop spectrophotometer (Peqlab, Erlangen, Germany).

    Article Title: Microscopic lymph node tumor burden quantified by macroscopic dual-tracer molecular imaging
    Article Snippet: Since a human breast cancer cell line was grown in a mouse model in this study, it was possible to use genetic markers to determine the ratio of cancer to normal tissue with real-time PCR based on established approaches., For improved sensitivity in this regard, the presence of luciferase gene expression was analyzed instead of a human specific gene, since the luciferase gene in not expressed naturally by mice but was transfected into the MDA-MB-231-luc-D3H2LN cell line by the manufacturer. .. Total RNA was isolated from flash frozen lymph nodes using RNeasy mini kit and Qiashredder (Qiagen, Venlo, Netherlands), and 18-gauge needles to homogenize the tissue. .. To assess gene expression levels, cDNA was prepared from lymph node RNA using the Superscript III First Strand cDNA Synthesis Kit and oligo dT primers (Life Technologies, Grand Island, NY).

    Article Title: Fibroblast-specific TGF- β–Smad2/3 signaling underlies cardiac fibrosis
    Article Snippet: The RNA-Seq data were deposited in the NCBI’s Gene Expression Omnibus (GEO ). .. RNA was isolated from fibroblasts using QIAshredder homogenization and the RNAeasy Kit according to the manufacturer’s instructions (QIAGEN, catalog 74104). .. Total RNA was reverse transcribed using random oligo-dT primers and a Verso cDNA Synthesis Kit (Thermo Fisher, AB1453) according to the manufacturer’s instructions.

    Article Title: The transcription factors Ik-1 and MZF1 downregulate IGF-IR expression in NPM-ALK+ T-cell lymphoma
    Article Snippet: Total RNA was isolated and purified using the RNAeasy Mini Kit (Qiagen). .. Briefly, 1 × 106 cells were collected by centrifugation at 200 g for 5 min, washed twice in phosphate-buffered saline (PBS), and subjected to lysis and homogenization with Buffer RLT using QiaShredder spin columns (Qiagen).

    Multiplex Assay:

    Article Title: A miRNA upregulated in asthma airway T cells promotes TH2 cytokine production
    Article Snippet: Paragraph title: High-throughput multiplex qPCR ... Frozen lysates were thawed and loaded onto QIAshredder homogenizer columns (Qiagen).

    Mouse Assay:

    Article Title: Microscopic lymph node tumor burden quantified by macroscopic dual-tracer molecular imaging
    Article Snippet: Since a human breast cancer cell line was grown in a mouse model in this study, it was possible to use genetic markers to determine the ratio of cancer to normal tissue with real-time PCR based on established approaches., For improved sensitivity in this regard, the presence of luciferase gene expression was analyzed instead of a human specific gene, since the luciferase gene in not expressed naturally by mice but was transfected into the MDA-MB-231-luc-D3H2LN cell line by the manufacturer. .. Total RNA was isolated from flash frozen lymph nodes using RNeasy mini kit and Qiashredder (Qiagen, Venlo, Netherlands), and 18-gauge needles to homogenize the tissue.

    Polymerase Chain Reaction:

    Article Title: Evaluation of cell damage induced by irradiated Zinc-Phthalocyanine-gold dendrimeric nanoparticles in a breast cancer cell line
    Article Snippet: An apoptosis positive control was prepared by inducing apoptosis with 1 μg/ml actinomycin D, and a necrosis positive control with 10% (v/v) hydrogen peroxide for 24 h. The real-time reverse transcriptase polymerase chain reaction (RT-PCR) was used to evaluate the expression of 84 genes following treatment (3 h incubation). .. Total RNA from untreated, MPDC-treated and PDT-treated cells was isolated using the RNeasy kit (Qiagen, 74104) with QIA shredder homogenizers (Qiagen, 79654).

    Article Title: AIM1 is an actin-binding protein that suppresses cell migration and micrometastatic dissemination
    Article Snippet: Personnel involved in animal experiments were blinded to the cell phenotype (knockdown vs. control) during engraftment and downstream analyses. .. PCR-based measurement of micrometastatic burden DNA extractions from frozen tissue samples were performed using QIAshredder columns for tissue homogenization and DNeasy extraction kits for DNA purification (Qiagen, Hilden, Germany) following manufacturer’s instructions. .. Extracted DNAs were quantitated using Qubit fluorometric quantification kits (Invitrogen) and quantitative real-time PCR.

    Article Title: Effects of Chronic Exposure to an Environmentally Relevant Mixture of Brominated Flame Retardants on the Reproductive and Thyroid System in Adult Male Rats
    Article Snippet: Frozen left testes (n = 9–10 per group) were disrupted using a mortar and pestle followed by homogenization via a 20 gauge needle and syringe combined with QIAshredder (Qiagen Inc.). .. Frozen left testes (n = 9–10 per group) were disrupted using a mortar and pestle followed by homogenization via a 20 gauge needle and syringe combined with QIAshredder (Qiagen Inc.).

    Article Title: Fibroblast-specific TGF- β–Smad2/3 signaling underlies cardiac fibrosis
    Article Snippet: Paragraph title: Reverse-transcriptase PCR. ... RNA was isolated from fibroblasts using QIAshredder homogenization and the RNAeasy Kit according to the manufacturer’s instructions (QIAGEN, catalog 74104).

    Construct:

    Article Title: Nanocomposite Scaffold for Chondrocyte Growth and Cartilage Tissue Engineering: Effects of Carbon Nanotube Surface Functionalization
    Article Snippet: Total RNA was isolated from hydrogel nanocomposite constructs using the RNEasy kit (Qiagen), where the lysis buffer was supplemented with β-mercaptoethanol (1%) and 2M sodium acetate (0.66%). .. Homogenization was performed with QIAShredder (Qiagen) according to the manufacturer's protocol.

    Lysis:

    Article Title: Human non-small cell lung cancer expresses putative cancer stem cell markers and exhibits the transcriptomic profile of multipotent cells
    Article Snippet: Total RNA was extracted from up to 1 × 106 CD166+ /CD44+ and CD166+ /EpCAM+ PHBEC, A549, and H2170 cells using the Qiagen AllPrep DNA/RNA Isolation Kit (Qiagen) according to the manufacturer’s protocol. .. Briefly, the cells were lysed with lysis buffer and homogenized using the QIAshredder Homogenizer (Qiagen). .. Ethanol (70%) was then added to the homogenized cell lysates, and the cell lysates were transferred into the RNA spin column.

    Article Title: Evidence that CED-9/Bcl2 and CED-4/Apaf-1 localization is not consistent with the current model for C. elegans apoptosis induction
    Article Snippet: Paragraph title: Lysis of worms and release of cytoplasmic content ... To further lyse the sample and to clear the lysate from non-lysed worms and worm cuticles, the sample was passed through cell-lysate homogenizer tubes (Qiashredder, Qiagen) and centrifuged for 2 min at 100 × r.c.f. (Eppendorf cooling benchtop centrifuge).

    Article Title: Transmitted/Founder Hepatitis C Viruses Induce Cell-Type- and Genotype-Specific Differences in Innate Signaling within the Liver
    Article Snippet: At 8 h posttransfection, cultures were lysed for gene expression analysis using RLT lysis buffer (Qiagen, Valencia, CA) with 10 µl β-mercaptoethanol added per ml of lysis buffer to stabilize RNA. .. The RNA was sent through QiaShredder homogenizing columns (Qiagen) and then stored at −80°C prior to further analysis using qRT-PCR.

    Article Title: Nanocomposite Scaffold for Chondrocyte Growth and Cartilage Tissue Engineering: Effects of Carbon Nanotube Surface Functionalization
    Article Snippet: Total RNA was isolated from hydrogel nanocomposite constructs using the RNEasy kit (Qiagen), where the lysis buffer was supplemented with β-mercaptoethanol (1%) and 2M sodium acetate (0.66%). .. Homogenization was performed with QIAShredder (Qiagen) according to the manufacturer's protocol.

    Article Title: The transcription factors Ik-1 and MZF1 downregulate IGF-IR expression in NPM-ALK+ T-cell lymphoma
    Article Snippet: Total RNA was isolated and purified using the RNAeasy Mini Kit (Qiagen). .. Briefly, 1 × 106 cells were collected by centrifugation at 200 g for 5 min, washed twice in phosphate-buffered saline (PBS), and subjected to lysis and homogenization with Buffer RLT using QiaShredder spin columns (Qiagen). .. Homogenized cells were re-suspended in an equal volume of 70% ethanol and passed through the spin columns.

    Purification:

    Article Title: The transcription factors Ik-1 and MZF1 downregulate IGF-IR expression in NPM-ALK+ T-cell lymphoma
    Article Snippet: Total RNA was isolated and purified using the RNAeasy Mini Kit (Qiagen). .. Briefly, 1 × 106 cells were collected by centrifugation at 200 g for 5 min, washed twice in phosphate-buffered saline (PBS), and subjected to lysis and homogenization with Buffer RLT using QiaShredder spin columns (Qiagen).

    RNA Extraction:

    Article Title: Regulation of glucose metabolism via hepatic forkhead transcription factor 1 (FoxO1) by Morinda citrifolia (noni) in high-fat diet-induced obese mice
    Article Snippet: Paragraph title: RNA extraction ... In brief, a PRO 200 Laboratory Homogenizer (Pro Scientific, Oxford, CT, USA), was used to homogenise 30-40 mg of liver, followed by the QIAshredder (catalogue no. 79656; Qiagen, Inc., Valencia, CA, USA), and RNA was extracted using the RNeasy Mini Kit (catalogue no. 74104; Qiagen, Inc.), according to the manufacturers’ protocol.

    Article Title: Human non-small cell lung cancer expresses putative cancer stem cell markers and exhibits the transcriptomic profile of multipotent cells
    Article Snippet: Paragraph title: Total RNA extraction and cDNA synthesis ... Briefly, the cells were lysed with lysis buffer and homogenized using the QIAshredder Homogenizer (Qiagen).

    Article Title: Effects of Chronic Exposure to an Environmentally Relevant Mixture of Brominated Flame Retardants on the Reproductive and Thyroid System in Adult Male Rats
    Article Snippet: Paragraph title: Testis RNA Extraction and Real-time Quantitative Reverse Transcription-PCR ... Frozen left testes (n = 9–10 per group) were disrupted using a mortar and pestle followed by homogenization via a 20 gauge needle and syringe combined with QIAshredder (Qiagen Inc.).

    Article Title: Comparison of Pre- and Post-translational Expressions of COXIV-1 and MT-ATPase 6 Genes in Colorectal Adenoma-Carcinoma Tissues
    Article Snippet: Paragraph title: RNA extraction and quantitative-PCR analysis ... Following tissue homogenization, samples were placed in QIAshredder (Qiagen, Germantown, MD, US) columns to reduce viscosity.

    Article Title: The transcription factors Ik-1 and MZF1 downregulate IGF-IR expression in NPM-ALK+ T-cell lymphoma
    Article Snippet: Paragraph title: RNA extraction, cDNA synthesis, and relative quantitative PCR (qPCR) ... Briefly, 1 × 106 cells were collected by centrifugation at 200 g for 5 min, washed twice in phosphate-buffered saline (PBS), and subjected to lysis and homogenization with Buffer RLT using QiaShredder spin columns (Qiagen).

    Software:

    Article Title: The secreted protein S100A7 (psoriasin) is induced by telomere dysfunction in human keratinocytes independently of a DNA damage response and cell cycle regulators
    Article Snippet: Extraction of total RNA was performed using the RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions, including sample homogenisation with QIAshredder (Qiagen) and DNase treatment with the RNase-free DNase Set (Qiagen). .. RT quantitative real-time PCR (absolute copy number quantification using standard curve for each gene) was performed using SYBR® green I Master (hot-start Taq polymerase master mix) in the LightCycler 480® qPCR system (Roche Applied Science, Welwyn Garden City, Herts., UK).

    Real-time Polymerase Chain Reaction:

    Article Title: AIM1 is an actin-binding protein that suppresses cell migration and micrometastatic dissemination
    Article Snippet: PCR-based measurement of micrometastatic burden DNA extractions from frozen tissue samples were performed using QIAshredder columns for tissue homogenization and DNeasy extraction kits for DNA purification (Qiagen, Hilden, Germany) following manufacturer’s instructions. .. Extracted DNAs were quantitated using Qubit fluorometric quantification kits (Invitrogen) and quantitative real-time PCR.

    Article Title: Chicken and Duck Myotubes Are Highly Susceptible and Permissive to Influenza Virus Infection
    Article Snippet: Paragraph title: Quantitative real-time PCR. ... Cells were homogenized with a QIAshredder homogenizer (Qiagen), followed by the removal of genomic DNA (gDNA) using a gDNA eliminator column.

    Article Title: An RNA Alternative to Human Transferrin: A New Tool for Targeting Human Cells
    Article Snippet: The second half of each sample was passed through a QIAshredder cell homogenizer (QIAGEN). .. Total cell RNA for each sample was isolated from the cell lysate according to QIAGEN's RNAeasy Plus Mini Kit protocol.

    Article Title: A miRNA upregulated in asthma airway T cells promotes TH2 cytokine production
    Article Snippet: Paragraph title: High-throughput multiplex qPCR ... Frozen lysates were thawed and loaded onto QIAshredder homogenizer columns (Qiagen).

    Article Title: Nanocomposite Scaffold for Chondrocyte Growth and Cartilage Tissue Engineering: Effects of Carbon Nanotube Surface Functionalization
    Article Snippet: Homogenization was performed with QIAShredder (Qiagen) according to the manufacturer's protocol. .. Homogenization was performed with QIAShredder (Qiagen) according to the manufacturer's protocol.

    Article Title: Effects of Chronic Exposure to an Environmentally Relevant Mixture of Brominated Flame Retardants on the Reproductive and Thyroid System in Adult Male Rats
    Article Snippet: Frozen left testes (n = 9–10 per group) were disrupted using a mortar and pestle followed by homogenization via a 20 gauge needle and syringe combined with QIAshredder (Qiagen Inc.). .. Frozen left testes (n = 9–10 per group) were disrupted using a mortar and pestle followed by homogenization via a 20 gauge needle and syringe combined with QIAshredder (Qiagen Inc.).

    Article Title: Microscopic lymph node tumor burden quantified by macroscopic dual-tracer molecular imaging
    Article Snippet: Paragraph title: Quantitative PCR for tumor burden validation ... Total RNA was isolated from flash frozen lymph nodes using RNeasy mini kit and Qiashredder (Qiagen, Venlo, Netherlands), and 18-gauge needles to homogenize the tissue.

    Article Title: Comparison of Pre- and Post-translational Expressions of COXIV-1 and MT-ATPase 6 Genes in Colorectal Adenoma-Carcinoma Tissues
    Article Snippet: Paragraph title: RNA extraction and quantitative-PCR analysis ... Following tissue homogenization, samples were placed in QIAshredder (Qiagen, Germantown, MD, US) columns to reduce viscosity.

    Article Title: Fibroblast-specific TGF- β–Smad2/3 signaling underlies cardiac fibrosis
    Article Snippet: RNA was isolated from fibroblasts using QIAshredder homogenization and the RNAeasy Kit according to the manufacturer’s instructions (QIAGEN, catalog 74104). .. RNA was isolated from fibroblasts using QIAshredder homogenization and the RNAeasy Kit according to the manufacturer’s instructions (QIAGEN, catalog 74104).

    Article Title: The secreted protein S100A7 (psoriasin) is induced by telomere dysfunction in human keratinocytes independently of a DNA damage response and cell cycle regulators
    Article Snippet: Paragraph title: qPCR ... Extraction of total RNA was performed using the RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions, including sample homogenisation with QIAshredder (Qiagen) and DNase treatment with the RNase-free DNase Set (Qiagen).

    Article Title: The transcription factors Ik-1 and MZF1 downregulate IGF-IR expression in NPM-ALK+ T-cell lymphoma
    Article Snippet: Paragraph title: RNA extraction, cDNA synthesis, and relative quantitative PCR (qPCR) ... Briefly, 1 × 106 cells were collected by centrifugation at 200 g for 5 min, washed twice in phosphate-buffered saline (PBS), and subjected to lysis and homogenization with Buffer RLT using QiaShredder spin columns (Qiagen).

    Negative Control:

    Article Title: The Transcriptional Complex Between the BCL2 i-Motif and hnRNP LL Is a Molecular Switch for Control of Gene Expression That Can Be Modulated by Small Molecules
    Article Snippet: After homogenization using QIAshredder (Qiagen), the extract was centrifuged at 14 00 rpm at 4 °C for 15 min. .. After homogenization using QIAshredder (Qiagen), the extract was centrifuged at 14 00 rpm at 4 °C for 15 min.

    In Vitro:

    Article Title: A miRNA upregulated in asthma airway T cells promotes TH2 cytokine production
    Article Snippet: Frozen lysates were thawed and loaded onto QIAshredder homogenizer columns (Qiagen). .. Frozen lysates were thawed and loaded onto QIAshredder homogenizer columns (Qiagen).

    Homogenization:

    Article Title: AIM1 is an actin-binding protein that suppresses cell migration and micrometastatic dissemination
    Article Snippet: Personnel involved in animal experiments were blinded to the cell phenotype (knockdown vs. control) during engraftment and downstream analyses. .. PCR-based measurement of micrometastatic burden DNA extractions from frozen tissue samples were performed using QIAshredder columns for tissue homogenization and DNeasy extraction kits for DNA purification (Qiagen, Hilden, Germany) following manufacturer’s instructions. .. Extracted DNAs were quantitated using Qubit fluorometric quantification kits (Invitrogen) and quantitative real-time PCR.

    Article Title: The Transcriptional Complex Between the BCL2 i-Motif and hnRNP LL Is a Molecular Switch for Control of Gene Expression That Can Be Modulated by Small Molecules
    Article Snippet: For hnRNP LL IP, whole-cell lysate was used. .. After homogenization using QIAshredder (Qiagen), the extract was centrifuged at 14 00 rpm at 4 °C for 15 min. .. The supernatant was precleaned with 50 μL of protein G-coupled magnetic beads (Invitrogen, 10003D) at 4 °C for 1 h. Protein concentration was determined by Bradford assay, and then the supernatant was diluted to 1 μg/μL for Sp1 and 12.5 μg/μL for hnRNP LL by lysis buffer.

    Article Title: Nanocomposite Scaffold for Chondrocyte Growth and Cartilage Tissue Engineering: Effects of Carbon Nanotube Surface Functionalization
    Article Snippet: Total RNA was isolated from hydrogel nanocomposite constructs using the RNEasy kit (Qiagen), where the lysis buffer was supplemented with β-mercaptoethanol (1%) and 2M sodium acetate (0.66%). .. Homogenization was performed with QIAShredder (Qiagen) according to the manufacturer's protocol. .. About 0.5–1 μg of RNA was reverse-transcribed into cDNA using the QuantiTect Reverse Transcription kit (Qiagen), which includes genomic DNA elimination.

    Article Title: Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat
    Article Snippet: After reaction clean up with the Qiaquick Gel Extraction Kit (Qiagen, Hilden, Germany) the PCR products containing the Rag1 mutation were cleaved by Sty I-HF (NEB, Frankfurt, Germany) into 603 bp and 449 bp, while the wild type Rag1 fragments remained unchanged. .. Total RNA was isolated from spleen, thymus, bone marrow and lymph nodes (mesenteric and cervical) from wild type LEW/Ztm rats, heterozygous and homozygous Rag1 mutant rats (n=10) using the RNeasy Mini Kit (Qiagen, Hilden, Germany) after homogenization of the tissues with the UP100H ultrasonic device (Hielscher, Teltow, Germany) and Qiashredder (Qiagen, Hilden, Germany). .. RNA concentrations were determined with the Nanodrop spectrophotometer (Peqlab, Erlangen, Germany).

    Article Title: Effects of Chronic Exposure to an Environmentally Relevant Mixture of Brominated Flame Retardants on the Reproductive and Thyroid System in Adult Male Rats
    Article Snippet: Relative gene expression was calculated using the CFX Manager Software, with RNA loading correction based on the expression of two housekeeping genes, beta actin (Actb) and hypoxanthine-guanine phosphoribosyltransferase (Hprt). .. Frozen left testes (n = 9–10 per group) were disrupted using a mortar and pestle followed by homogenization via a 20 gauge needle and syringe combined with QIAshredder (Qiagen Inc.). .. RNA extraction was carried out using an RNeasy Plus Mini Kit with on-column DNase digestion (Qiagen Inc.) according to the manufacturer’s protocol.

    Article Title: Comparison of Pre- and Post-translational Expressions of COXIV-1 and MT-ATPase 6 Genes in Colorectal Adenoma-Carcinoma Tissues
    Article Snippet: Briefly, frozen samples were cut on ice and washed with PBS twice before following the manufacturer’s protocol. .. Following tissue homogenization, samples were placed in QIAshredder (Qiagen, Germantown, MD, US) columns to reduce viscosity. .. Purity of the RNA was assessed by 1% agarose gel electrophoresis and quantified with a Nanodrop ND-2000 spectrophotometer (Nanodrop Technologies, Wilmington, DE, US).

    Article Title: Fibroblast-specific TGF- β–Smad2/3 signaling underlies cardiac fibrosis
    Article Snippet: The RNA-Seq data were deposited in the NCBI’s Gene Expression Omnibus (GEO ). .. RNA was isolated from fibroblasts using QIAshredder homogenization and the RNAeasy Kit according to the manufacturer’s instructions (QIAGEN, catalog 74104). .. Total RNA was reverse transcribed using random oligo-dT primers and a Verso cDNA Synthesis Kit (Thermo Fisher, AB1453) according to the manufacturer’s instructions.

    Article Title: The secreted protein S100A7 (psoriasin) is induced by telomere dysfunction in human keratinocytes independently of a DNA damage response and cell cycle regulators
    Article Snippet: Selective medium was then replaced with FAD overnight and cell pellets collected by centrifugation. .. Extraction of total RNA was performed using the RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions, including sample homogenisation with QIAshredder (Qiagen) and DNase treatment with the RNase-free DNase Set (Qiagen). .. Purified RNA was reverse transcribed using with Finnzymes DyNAmo™ cDNA Synthesis Kit (New England BioLabs, Hitchin, UK) and the cDNA stored at -20°C.

    Article Title: The transcription factors Ik-1 and MZF1 downregulate IGF-IR expression in NPM-ALK+ T-cell lymphoma
    Article Snippet: Total RNA was isolated and purified using the RNAeasy Mini Kit (Qiagen). .. Briefly, 1 × 106 cells were collected by centrifugation at 200 g for 5 min, washed twice in phosphate-buffered saline (PBS), and subjected to lysis and homogenization with Buffer RLT using QiaShredder spin columns (Qiagen). .. Homogenized cells were re-suspended in an equal volume of 70% ethanol and passed through the spin columns.

    Incubation:

    Article Title: Evaluation of cell damage induced by irradiated Zinc-Phthalocyanine-gold dendrimeric nanoparticles in a breast cancer cell line
    Article Snippet: An apoptosis positive control was prepared by inducing apoptosis with 1 μg/ml actinomycin D, and a necrosis positive control with 10% (v/v) hydrogen peroxide for 24 h. The real-time reverse transcriptase polymerase chain reaction (RT-PCR) was used to evaluate the expression of 84 genes following treatment (3 h incubation). .. Total RNA from untreated, MPDC-treated and PDT-treated cells was isolated using the RNeasy kit (Qiagen, 74104) with QIA shredder homogenizers (Qiagen, 79654).

    Article Title: Evidence that CED-9/Bcl2 and CED-4/Apaf-1 localization is not consistent with the current model for C. elegans apoptosis induction
    Article Snippet: To further lyse the sample and to clear the lysate from non-lysed worms and worm cuticles, the sample was passed through cell-lysate homogenizer tubes (Qiashredder, Qiagen) and centrifuged for 2 min at 100 × r.c.f. (Eppendorf cooling benchtop centrifuge). .. To further lyse the sample and to clear the lysate from non-lysed worms and worm cuticles, the sample was passed through cell-lysate homogenizer tubes (Qiashredder, Qiagen) and centrifuged for 2 min at 100 × r.c.f. (Eppendorf cooling benchtop centrifuge).

    Article Title: The Transcriptional Complex Between the BCL2 i-Motif and hnRNP LL Is a Molecular Switch for Control of Gene Expression That Can Be Modulated by Small Molecules
    Article Snippet: For Sp1 IP, nuclei isolated by kit (Sigma, NUC101) were incubated with RIPA buffer (Cell Signaling, #9806) for 15 min on ice. .. After homogenization using QIAshredder (Qiagen), the extract was centrifuged at 14 00 rpm at 4 °C for 15 min.

    Spectrophotometry:

    Article Title: Human non-small cell lung cancer expresses putative cancer stem cell markers and exhibits the transcriptomic profile of multipotent cells
    Article Snippet: Briefly, the cells were lysed with lysis buffer and homogenized using the QIAshredder Homogenizer (Qiagen). .. Briefly, the cells were lysed with lysis buffer and homogenized using the QIAshredder Homogenizer (Qiagen).

    Article Title: The transcription factors Ik-1 and MZF1 downregulate IGF-IR expression in NPM-ALK+ T-cell lymphoma
    Article Snippet: Briefly, 1 × 106 cells were collected by centrifugation at 200 g for 5 min, washed twice in phosphate-buffered saline (PBS), and subjected to lysis and homogenization with Buffer RLT using QiaShredder spin columns (Qiagen). .. Briefly, 1 × 106 cells were collected by centrifugation at 200 g for 5 min, washed twice in phosphate-buffered saline (PBS), and subjected to lysis and homogenization with Buffer RLT using QiaShredder spin columns (Qiagen).

    Sampling:

    Article Title: Momordica charantia (bitter melon) attenuates high-fat diet-associated oxidative stress and neuroinflammation
    Article Snippet: Total dye was calculated based on external standards (10 -1000 ng/mL) dissolved in 50% TCA/ethanol (1:3). .. RNA extractions Frozen left brains were powdered to obtain a homogenous sampling and 30 to 40 mg of whole brain was homogenized using PRO 200 Laboratory Homogenizer (Pro Scientific, Oxford, CT) followed by the QIAshredder (Qiagen, Inc., Valencia, CA, Cat#: 79656). .. RNA was extracted using the RNeasy Mini Kit (Qiagen, Inc., Cat#: 74104) as per the manufacturers' protocol.

    Immunoprecipitation:

    Article Title: The Transcriptional Complex Between the BCL2 i-Motif and hnRNP LL Is a Molecular Switch for Control of Gene Expression That Can Be Modulated by Small Molecules
    Article Snippet: Paragraph title: Immunoprecipitation (IP) ... After homogenization using QIAshredder (Qiagen), the extract was centrifuged at 14 00 rpm at 4 °C for 15 min.

    Multiple Displacement Amplification:

    Article Title: Microscopic lymph node tumor burden quantified by macroscopic dual-tracer molecular imaging
    Article Snippet: Since a human breast cancer cell line was grown in a mouse model in this study, it was possible to use genetic markers to determine the ratio of cancer to normal tissue with real-time PCR based on established approaches., For improved sensitivity in this regard, the presence of luciferase gene expression was analyzed instead of a human specific gene, since the luciferase gene in not expressed naturally by mice but was transfected into the MDA-MB-231-luc-D3H2LN cell line by the manufacturer. .. Total RNA was isolated from flash frozen lymph nodes using RNeasy mini kit and Qiashredder (Qiagen, Venlo, Netherlands), and 18-gauge needles to homogenize the tissue.

    DNA Purification:

    Article Title: AIM1 is an actin-binding protein that suppresses cell migration and micrometastatic dissemination
    Article Snippet: Personnel involved in animal experiments were blinded to the cell phenotype (knockdown vs. control) during engraftment and downstream analyses. .. PCR-based measurement of micrometastatic burden DNA extractions from frozen tissue samples were performed using QIAshredder columns for tissue homogenization and DNeasy extraction kits for DNA purification (Qiagen, Hilden, Germany) following manufacturer’s instructions. .. Extracted DNAs were quantitated using Qubit fluorometric quantification kits (Invitrogen) and quantitative real-time PCR.

    Marker:

    Article Title: Nanocomposite Scaffold for Chondrocyte Growth and Cartilage Tissue Engineering: Effects of Carbon Nanotube Surface Functionalization
    Article Snippet: Gene expression of ECM genes (aggrecan, collagen-1, collagen-2, and collagen-10), surface adhesion proteins (fibronectin, CD44), and tumor marker (p53) levels was determined by quantitative RT-PCR from chondrocytes cultured in hydrogel nanocomposite scaffolds. .. Homogenization was performed with QIAShredder (Qiagen) according to the manufacturer's protocol.

    Staining:

    Article Title: Evaluation of cell damage induced by irradiated Zinc-Phthalocyanine-gold dendrimeric nanoparticles in a breast cancer cell line
    Article Snippet: Several control samples were included and prepared for the assay as follows: cells only without any stain; cells stained with Annexin V-FITC only; cells stained with PI only; and positive control cells which included those stained with both Annexin V-FITC and PI (late apoptotic). .. Total RNA from untreated, MPDC-treated and PDT-treated cells was isolated using the RNeasy kit (Qiagen, 74104) with QIA shredder homogenizers (Qiagen, 79654).

    Variant Assay:

    Article Title: The secreted protein S100A7 (psoriasin) is induced by telomere dysfunction in human keratinocytes independently of a DNA damage response and cell cycle regulators
    Article Snippet: Extraction of total RNA was performed using the RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions, including sample homogenisation with QIAshredder (Qiagen) and DNase treatment with the RNase-free DNase Set (Qiagen). .. Target gene relative expression levels were calculated in relation to keratinocyte reference genes POLR2A and YAP1 [ ] using the LightCycler 480® Relative Quantification Software (with built-in multiple reference genes normalisation algorithm).

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    Qiagen qiashredder column
    Analysis of technical bias to perceived community composition. (A) NMDS of the 16S rRNA gene amplicon sequencing data based on a Bray-Curtis dissimilarity matrix generated after random subsampling of 2,800 sequences from each sample. Bars indicate the range of coordinates for the three replicate extractions/sequencing datasets per treatment. (B) Phylum level data (top 10 most abundant phyla, fractions of all reads). Number 1–3 between parentheses indicates the sequencing run from which each dataset is derived. APO combines three slightly different treatments that did not result in significantly different taxonomic representations ( S2 Fig .). Acronyms: Extraction protocols: APS (standard AllPrep protocol), APO (optimized AllPrep protocol), Enz (Enzymatic protocol), Bead (Bead-beating protocol); Preservation methods: NT (none), RL (RNAlater), RP (RNAprotect), BU (Qiagen Lysis Buffer RLT+); Other modifications: LYS (lysozyme), NQ (No <t>QIAshredder</t> column), TL (bead-beating with TissueLyser). Except for the APO samples, none of the Douglas Lake filters were preserved in RNA protection agents.
    Qiashredder Column, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 679 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiashredder column/product/Qiagen
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    qiashredder column - by Bioz Stars, 2019-12
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    77
    Qiagen qiaschredder column
    Analysis of technical bias to perceived community composition. (A) NMDS of the 16S rRNA gene amplicon sequencing data based on a Bray-Curtis dissimilarity matrix generated after random subsampling of 2,800 sequences from each sample. Bars indicate the range of coordinates for the three replicate extractions/sequencing datasets per treatment. (B) Phylum level data (top 10 most abundant phyla, fractions of all reads). Number 1–3 between parentheses indicates the sequencing run from which each dataset is derived. APO combines three slightly different treatments that did not result in significantly different taxonomic representations ( S2 Fig .). Acronyms: Extraction protocols: APS (standard AllPrep protocol), APO (optimized AllPrep protocol), Enz (Enzymatic protocol), Bead (Bead-beating protocol); Preservation methods: NT (none), RL (RNAlater), RP (RNAprotect), BU (Qiagen Lysis Buffer RLT+); Other modifications: LYS (lysozyme), NQ (No <t>QIAshredder</t> column), TL (bead-beating with TissueLyser). Except for the APO samples, none of the Douglas Lake filters were preserved in RNA protection agents.
    Qiaschredder Column, supplied by Qiagen, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaschredder column/product/Qiagen
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    qiaschredder column - by Bioz Stars, 2019-12
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    Analysis of technical bias to perceived community composition. (A) NMDS of the 16S rRNA gene amplicon sequencing data based on a Bray-Curtis dissimilarity matrix generated after random subsampling of 2,800 sequences from each sample. Bars indicate the range of coordinates for the three replicate extractions/sequencing datasets per treatment. (B) Phylum level data (top 10 most abundant phyla, fractions of all reads). Number 1–3 between parentheses indicates the sequencing run from which each dataset is derived. APO combines three slightly different treatments that did not result in significantly different taxonomic representations ( S2 Fig .). Acronyms: Extraction protocols: APS (standard AllPrep protocol), APO (optimized AllPrep protocol), Enz (Enzymatic protocol), Bead (Bead-beating protocol); Preservation methods: NT (none), RL (RNAlater), RP (RNAprotect), BU (Qiagen Lysis Buffer RLT+); Other modifications: LYS (lysozyme), NQ (No QIAshredder column), TL (bead-beating with TissueLyser). Except for the APO samples, none of the Douglas Lake filters were preserved in RNA protection agents.

    Journal: PLoS ONE

    Article Title: RNA Preservation Agents and Nucleic Acid Extraction Method Bias Perceived Bacterial Community Composition

    doi: 10.1371/journal.pone.0121659

    Figure Lengend Snippet: Analysis of technical bias to perceived community composition. (A) NMDS of the 16S rRNA gene amplicon sequencing data based on a Bray-Curtis dissimilarity matrix generated after random subsampling of 2,800 sequences from each sample. Bars indicate the range of coordinates for the three replicate extractions/sequencing datasets per treatment. (B) Phylum level data (top 10 most abundant phyla, fractions of all reads). Number 1–3 between parentheses indicates the sequencing run from which each dataset is derived. APO combines three slightly different treatments that did not result in significantly different taxonomic representations ( S2 Fig .). Acronyms: Extraction protocols: APS (standard AllPrep protocol), APO (optimized AllPrep protocol), Enz (Enzymatic protocol), Bead (Bead-beating protocol); Preservation methods: NT (none), RL (RNAlater), RP (RNAprotect), BU (Qiagen Lysis Buffer RLT+); Other modifications: LYS (lysozyme), NQ (No QIAshredder column), TL (bead-beating with TissueLyser). Except for the APO samples, none of the Douglas Lake filters were preserved in RNA protection agents.

    Article Snippet: The lysate was transferred to a QIAshredder column (Qiagen) and the remainder of the protocol was performed according to the manufacturer’s instructions, performing two elution steps with of 30 μl elution buffer for both RNA and DNA.

    Techniques: Amplification, Sequencing, Generated, Derivative Assay, Preserving, Lysis

    View of threshold (Ct) values derived from real-time PCR. Ct values were compared among PCR amplifications using three preparation methods and two primer sets. In each of 11 Las-infected citrus leaf samples, real-time PCR using Las606/LSS and OI1/OI2c primer sets with templates obtained from Extracted DNA , Biomasher-pellet , and QIAshredder-pellet was performed to derive each Ct value. The numbers in each graph are Ct values.

    Journal: PLoS ONE

    Article Title: Convenient Detection of the Citrus Greening (Huanglongbing) Bacterium 'Candidatus Liberibacter asiaticus' by Direct PCR from the Midrib Extract

    doi: 10.1371/journal.pone.0057011

    Figure Lengend Snippet: View of threshold (Ct) values derived from real-time PCR. Ct values were compared among PCR amplifications using three preparation methods and two primer sets. In each of 11 Las-infected citrus leaf samples, real-time PCR using Las606/LSS and OI1/OI2c primer sets with templates obtained from Extracted DNA , Biomasher-pellet , and QIAshredder-pellet was performed to derive each Ct value. The numbers in each graph are Ct values.

    Article Snippet: Next, they were shredded using a QIAshredder spin column (QIAGEN) with centrifugation for 2 min at 5000×g .

    Techniques: Derivative Assay, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Infection