qiaquick 96 pcr purification plate  (Qiagen)


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    QIAquick 96 PCR Purification Kit
    Description:
    For purification of 96 PCR products up to 10 μg 100 bp to 10 kb Kit contents Qiagen QIAquick 96 PCR Purification Kit 4 x 96 rxns 10g Binding Capacity 60 to 80L Elution Volume 96 well Plate Format Manual Automated Processing Silica Technology 100 bp to 10 kb Fragment For Purification of 96 PCR Products up to 10μg Ideal for Sequencing Microarray Analysis Ligation and Transformation Restriction Digestion Labeling Microinjection PCR and In Vitro Transcription Includes 4 QIAquick 96 Plates Buffers Collection Microtubes 1 2mL Caps Benefits Up to 95 recovery of ready to use DNA Fast and convenient procedure Cleanup of DNA up to 10 kb in three easy step
    Catalog Number:
    28181
    Price:
    831
    Category:
    QIAquick 96 PCR Purification Kits
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    Structured Review

    Qiagen qiaquick 96 pcr purification plate
    QIAquick 96 PCR Purification Kit
    For purification of 96 PCR products up to 10 μg 100 bp to 10 kb Kit contents Qiagen QIAquick 96 PCR Purification Kit 4 x 96 rxns 10g Binding Capacity 60 to 80L Elution Volume 96 well Plate Format Manual Automated Processing Silica Technology 100 bp to 10 kb Fragment For Purification of 96 PCR Products up to 10μg Ideal for Sequencing Microarray Analysis Ligation and Transformation Restriction Digestion Labeling Microinjection PCR and In Vitro Transcription Includes 4 QIAquick 96 Plates Buffers Collection Microtubes 1 2mL Caps Benefits Up to 95 recovery of ready to use DNA Fast and convenient procedure Cleanup of DNA up to 10 kb in three easy step
    https://www.bioz.com/result/qiaquick 96 pcr purification plate/product/Qiagen
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    qiaquick 96 pcr purification plate - by Bioz Stars, 2020-04
    99/100 stars

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    Clone Assay:

    Article Title: Genetic Diversity of Eurycoma longifolia Inferred from Single Nucleotide Polymorphisms 1 Inferred from Single Nucleotide Polymorphisms 1 [w]
    Article Snippet: Paragraph title: End Sequencing of Cloned DOP-PCR Products ... The entire products were loaded onto 1.1% (w/v) agarose gel, electrophoresed, and gel purified using Qiaquick 96 PCR purification kit (Qiagen USA).

    Article Title: High-throughput Protein Purification and Quality Assessment for Crystallization
    Article Snippet: Paragraph title: 3.1. Methods: gene amplification and cloning ... After the thermocycler program is completed, the amplified fragments are purified using a QIAquick 96 PCR fragment purification kit (Qiagen, Inc., Valencia, CA).

    Amplification:

    Article Title: Rapid Phenotypic and Genomic Change in Response to Therapeutic Pressure in Prostate Cancer Inferred by High Content Analysis of Single Circulating Tumor Cells
    Article Snippet: WGA was carried out manually in a 96-well plate format using the WGA4 Genomeplex Single Cell Whole Genome Amplification Kit (Sigma-Aldrich), followed by purification using a QIAquick 96 PCR Purification Kit (Qiagen). .. For each well, amplification was considered successful if the resulting DNA concentration was ≥70 ng/ µl (elution volume of 50 µl), followed by further Quality Control (QC) to confirm the appropriate sample size distribution using the Agilent 2100 Bioanalyzer (High-Sensitivity DNA Assay and Kit, Agilent Technologies).

    Article Title: High-throughput Protein Purification and Quality Assessment for Crystallization
    Article Snippet: .. After the thermocycler program is completed, the amplified fragments are purified using a QIAquick 96 PCR fragment purification kit (Qiagen, Inc., Valencia, CA). .. The semi-automated fragment purification method uses the solutions provided in the kit and procedures modified to adapt to the FX instrument (Beckman Coulter, Inc.).

    Article Title: Extensive sequence variation in the 3′ untranslated region of the KRAS gene in lung and ovarian cancer cases
    Article Snippet: Segments of the KRAS 3′ UTR were amplified using Pfu Turbo DNA polymerase (Stratagene) or Phusion high-fidelity DNA polymerase (NEB) and DNA primers specific to the sequence ( ). .. PCR products were purified using the QIAquick PCR purification kit, 96-PCR purification kit (Qiagen), or at the core sequencing laboratory at the W.M.

    Article Title: Genome wide copy number analysis of single cells
    Article Snippet: Whole Genome Amplifications Kit-WGA4 (Sigma-Aldrich, cat. no: WGA4-50RXN) CRITICAL Sigma-Aldrich WGA4 kits yields relatively uniform distributions of amplification products across the genome to facilitate copy number analysis using the SNS method. .. QIAquick 96 well purification kit (Qiagen, cat. no: 28181) .

    Article Title: A new sensitive PCR assay for one-step detection of 12 IDH1/2 mutations in glioma
    Article Snippet: A fragment containing IDH1 codon 100 was amplified using the following primers: 5’-AAGGATGCTGCAGAAGCTATAA-3’ (forward) and 5’-CCATAAGCATGACGACCTATGA-3’ (reverse). .. PCR products were purified using the QIAquick 96 PCR Purification kit (Qiagen, Hilden).

    Whole Genome Amplification:

    Article Title: Rapid Phenotypic and Genomic Change in Response to Therapeutic Pressure in Prostate Cancer Inferred by High Content Analysis of Single Circulating Tumor Cells
    Article Snippet: .. WGA was carried out manually in a 96-well plate format using the WGA4 Genomeplex Single Cell Whole Genome Amplification Kit (Sigma-Aldrich), followed by purification using a QIAquick 96 PCR Purification Kit (Qiagen). ..

    Synthesized:

    Article Title: High-throughput Protein Purification and Quality Assessment for Crystallization
    Article Snippet: The oligos are commercially synthesized in a 96-well plate format. .. After the thermocycler program is completed, the amplified fragments are purified using a QIAquick 96 PCR fragment purification kit (Qiagen, Inc., Valencia, CA).

    Electrophoresis:

    Article Title: Reduced-Median-Network Analysis of Complete Mitochondrial DNA Coding-Region Sequences for the Major African, Asian, and European Haplogroups
    Article Snippet: Triplicate reactions were pooled and purified with the QIAquick 96 PCR Purification Kit (Qiagen). .. Electrophoresis and base “calling” were performed using a 3700 DNA Analyzer (PE Applied Biosystems).

    Article Title: A new sensitive PCR assay for one-step detection of 12 IDH1/2 mutations in glioma
    Article Snippet: PCR products were purified using the QIAquick 96 PCR Purification kit (Qiagen, Hilden). .. The purified PCR products (1.5 μL) were sequenced using Applied Biosystems Big Dye 3.1 dideoxy chain termination sequencing chemistry in conjunction with fluorescent-based capillary sequencers (Model 3730xl Applied Biosystems Division of Perkin Elmer, Foster City, California, USA) for electrophoresis, data collection and base calling.

    Incubation:

    Article Title: Population sequencing of two endocannabinoid metabolic genes identifies rare and common regulatory variants associated with extreme obesity and metabolite level
    Article Snippet: The fragmented DNA was purified in a Qiaquick 96 PCR purification plate (QIAGEN, Valencia, CA, USA) following the manufacturer's instructions. .. The reaction was incubated for 30 minutes at 20°C and the DNA purified on a Qiaquick 96 PCR purification plate.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Extensive sequence variation in the 3′ untranslated region of the KRAS gene in lung and ovarian cancer cases
    Article Snippet: DNA was isolated from frozen and FFPE lung tissue using the Dneasy Blood and Tissue kit (Qiagen). .. PCR products were purified using the QIAquick PCR purification kit, 96-PCR purification kit (Qiagen), or at the core sequencing laboratory at the W.M.

    Touchdown PCR:

    Article Title: High-throughput Protein Purification and Quality Assessment for Crystallization
    Article Snippet: The touchdown PCR cycling parameters are as follows: initial denaturation at 95°C for 2 min and 30 sec, primer annealing for 30 sec, and elongation at 70°C for 3 min. .. After the thermocycler program is completed, the amplified fragments are purified using a QIAquick 96 PCR fragment purification kit (Qiagen, Inc., Valencia, CA).

    Modification:

    Article Title: High-throughput Protein Purification and Quality Assessment for Crystallization
    Article Snippet: After the thermocycler program is completed, the amplified fragments are purified using a QIAquick 96 PCR fragment purification kit (Qiagen, Inc., Valencia, CA). .. The semi-automated fragment purification method uses the solutions provided in the kit and procedures modified to adapt to the FX instrument (Beckman Coulter, Inc.).

    Degenerate Oligonucleotide–primed Polymerase Chain Reaction:

    Article Title: Genetic Diversity of Eurycoma longifolia Inferred from Single Nucleotide Polymorphisms 1 Inferred from Single Nucleotide Polymorphisms 1 [w]
    Article Snippet: Paragraph title: End Sequencing of Cloned DOP-PCR Products ... The entire products were loaded onto 1.1% (w/v) agarose gel, electrophoresed, and gel purified using Qiaquick 96 PCR purification kit (Qiagen USA).

    DNA Sequencing:

    Article Title: Reduced-Median-Network Analysis of Complete Mitochondrial DNA Coding-Region Sequences for the Major African, Asian, and European Haplogroups
    Article Snippet: Paragraph title: DNA Sequencing ... Triplicate reactions were pooled and purified with the QIAquick 96 PCR Purification Kit (Qiagen).

    Polymerase Chain Reaction:

    Article Title: Recent Acquisition of Helicobacter pylori by Baka Pygmies
    Article Snippet: .. PCR products were purified using the QIAquick 96 PCR Purification Kit (Qiagen). .. PCR amplicons were sequenced bidirectionally using the BigDye Terminator v1.1 Cycle Sequencing Kit and the 3130xl Genetic Analyzer (Applied Biosystems).

    Article Title: Rapid Phenotypic and Genomic Change in Response to Therapeutic Pressure in Prostate Cancer Inferred by High Content Analysis of Single Circulating Tumor Cells
    Article Snippet: .. WGA was carried out manually in a 96-well plate format using the WGA4 Genomeplex Single Cell Whole Genome Amplification Kit (Sigma-Aldrich), followed by purification using a QIAquick 96 PCR Purification Kit (Qiagen). ..

    Article Title: Genetic Diversity of Eurycoma longifolia Inferred from Single Nucleotide Polymorphisms 1 Inferred from Single Nucleotide Polymorphisms 1 [w]
    Article Snippet: .. The entire products were loaded onto 1.1% (w/v) agarose gel, electrophoresed, and gel purified using Qiaquick 96 PCR purification kit (Qiagen USA). .. The purified PCR products were sent for automated sequencing at MWG Biotech Inc. (High Point, NC).

    Article Title: Host Life History Strategy, Species Diversity, and Habitat Influence Trypanosoma cruzi Vector Infection in Changing Landscapes
    Article Snippet: .. PCR products were then purified with a high throughput adaptation of gel extraction followed by vacuum manifold PCR product purification using a QIAquick 96 PCR Purification Kit (Qiagen, Valencia, CA 91355) following manufacturer's instructions. .. After purification, PCR products were then tested for purity and DNA concentration with a Nanodrop spectrophotometer and sent for sequencing to the University of Georgia Bioinformatics laboratory.

    Article Title: Reduced-Median-Network Analysis of Complete Mitochondrial DNA Coding-Region Sequences for the Major African, Asian, and European Haplogroups
    Article Snippet: .. Triplicate reactions were pooled and purified with the QIAquick 96 PCR Purification Kit (Qiagen). .. Sequencing reactions were performed with 3 μl of PCR product, forward or reverse PCR primer, and BigDyeTerminator chemistry (PE Applied Biosystems).

    Article Title: High-throughput Protein Purification and Quality Assessment for Crystallization
    Article Snippet: .. After the thermocycler program is completed, the amplified fragments are purified using a QIAquick 96 PCR fragment purification kit (Qiagen, Inc., Valencia, CA). .. The semi-automated fragment purification method uses the solutions provided in the kit and procedures modified to adapt to the FX instrument (Beckman Coulter, Inc.).

    Article Title: A Highly Reproducible, Linear, and Automated Sample Preparation Method for DNA Microarrays
    Article Snippet: .. All liquid and enzyme solution additions and purifications were performed on a QIAGEN BioRobot (model 9604 or 3000). cDNA purification and aRNA purification were performed using QIAquick 96 PCR purification kits and RNeasy96 kits (QIAGEN), respectively. ..

    Article Title: Extensive sequence variation in the 3′ untranslated region of the KRAS gene in lung and ovarian cancer cases
    Article Snippet: .. PCR products were purified using the QIAquick PCR purification kit, 96-PCR purification kit (Qiagen), or at the core sequencing laboratory at the W.M. ..

    Article Title: Population sequencing of two endocannabinoid metabolic genes identifies rare and common regulatory variants associated with extreme obesity and metabolite level
    Article Snippet: .. The fragmented DNA was purified in a Qiaquick 96 PCR purification plate (QIAGEN, Valencia, CA, USA) following the manufacturer's instructions. .. The DNA ends were repaired in a 100 μl reaction (1× NEB ligase buffer, 1 mg/ml bovine serum albumin, 200 μM dNTP) using 15 U T4 DNA polymerase (NEB Ipswich, MA, USA), 50 U T4 polynucleotide kinase (NEB), and 5 U Klenow DNA polymerase.

    Article Title: A new sensitive PCR assay for one-step detection of 12 IDH1/2 mutations in glioma
    Article Snippet: .. PCR products were purified using the QIAquick 96 PCR Purification kit (Qiagen, Hilden). .. The purified PCR products (1.5 μL) were sequenced using Applied Biosystems Big Dye 3.1 dideoxy chain termination sequencing chemistry in conjunction with fluorescent-based capillary sequencers (Model 3730xl Applied Biosystems Division of Perkin Elmer, Foster City, California, USA) for electrophoresis, data collection and base calling.

    DNA Extraction:

    Article Title: Extensive sequence variation in the 3′ untranslated region of the KRAS gene in lung and ovarian cancer cases
    Article Snippet: Paragraph title: DNA isolation and direct Sanger sequencing for NSCLC cases ... PCR products were purified using the QIAquick PCR purification kit, 96-PCR purification kit (Qiagen), or at the core sequencing laboratory at the W.M.

    Fluorescence:

    Article Title: High-throughput Protein Purification and Quality Assessment for Crystallization
    Article Snippet: After the thermocycler program is completed, the amplified fragments are purified using a QIAquick 96 PCR fragment purification kit (Qiagen, Inc., Valencia, CA). .. The DNA concentration of the purified fragments is determined by a PicoGreen fluorescent assay (Invitrogen/Molecular Probes, Eugene, OR) using 96-well plates.

    Mutagenesis:

    Article Title: A new sensitive PCR assay for one-step detection of 12 IDH1/2 mutations in glioma
    Article Snippet: PCR products were purified using the QIAquick 96 PCR Purification kit (Qiagen, Hilden). .. Locked Nucleic Acid (LNA) Sanger sequencing was performed in a separate reaction by amplifying the mutation hotspot regions using the pyrosequencing PCR primers and LNA (+T + A + G + G + T + C + G + T + C + A+/3InvdT/) at the final concentration of 70 nM.

    Isolation:

    Article Title: Recent Acquisition of Helicobacter pylori by Baka Pygmies
    Article Snippet: Molecular genetics Genomic DNA of bacterial strains was isolated using the QIAamp DNA Mini Kit (Qiagen). .. PCR products were purified using the QIAquick 96 PCR Purification Kit (Qiagen).

    Article Title: Extensive sequence variation in the 3′ untranslated region of the KRAS gene in lung and ovarian cancer cases
    Article Snippet: DNA was isolated from frozen and FFPE lung tissue using the Dneasy Blood and Tissue kit (Qiagen). .. PCR products were purified using the QIAquick PCR purification kit, 96-PCR purification kit (Qiagen), or at the core sequencing laboratory at the W.M.

    Size-exclusion Chromatography:

    Article Title: High-throughput Protein Purification and Quality Assessment for Crystallization
    Article Snippet: The touchdown PCR cycling parameters are as follows: initial denaturation at 95°C for 2 min and 30 sec, primer annealing for 30 sec, and elongation at 70°C for 3 min. .. After the thermocycler program is completed, the amplified fragments are purified using a QIAquick 96 PCR fragment purification kit (Qiagen, Inc., Valencia, CA).

    Purification:

    Article Title: Recent Acquisition of Helicobacter pylori by Baka Pygmies
    Article Snippet: .. PCR products were purified using the QIAquick 96 PCR Purification Kit (Qiagen). .. PCR amplicons were sequenced bidirectionally using the BigDye Terminator v1.1 Cycle Sequencing Kit and the 3130xl Genetic Analyzer (Applied Biosystems).

    Article Title: Rapid Phenotypic and Genomic Change in Response to Therapeutic Pressure in Prostate Cancer Inferred by High Content Analysis of Single Circulating Tumor Cells
    Article Snippet: .. WGA was carried out manually in a 96-well plate format using the WGA4 Genomeplex Single Cell Whole Genome Amplification Kit (Sigma-Aldrich), followed by purification using a QIAquick 96 PCR Purification Kit (Qiagen). ..

    Article Title: Genetic Diversity of Eurycoma longifolia Inferred from Single Nucleotide Polymorphisms 1 Inferred from Single Nucleotide Polymorphisms 1 [w]
    Article Snippet: .. The entire products were loaded onto 1.1% (w/v) agarose gel, electrophoresed, and gel purified using Qiaquick 96 PCR purification kit (Qiagen USA). .. The purified PCR products were sent for automated sequencing at MWG Biotech Inc. (High Point, NC).

    Article Title: Host Life History Strategy, Species Diversity, and Habitat Influence Trypanosoma cruzi Vector Infection in Changing Landscapes
    Article Snippet: .. PCR products were then purified with a high throughput adaptation of gel extraction followed by vacuum manifold PCR product purification using a QIAquick 96 PCR Purification Kit (Qiagen, Valencia, CA 91355) following manufacturer's instructions. .. After purification, PCR products were then tested for purity and DNA concentration with a Nanodrop spectrophotometer and sent for sequencing to the University of Georgia Bioinformatics laboratory.

    Article Title: Reduced-Median-Network Analysis of Complete Mitochondrial DNA Coding-Region Sequences for the Major African, Asian, and European Haplogroups
    Article Snippet: .. Triplicate reactions were pooled and purified with the QIAquick 96 PCR Purification Kit (Qiagen). .. Sequencing reactions were performed with 3 μl of PCR product, forward or reverse PCR primer, and BigDyeTerminator chemistry (PE Applied Biosystems).

    Article Title: High-throughput Protein Purification and Quality Assessment for Crystallization
    Article Snippet: .. After the thermocycler program is completed, the amplified fragments are purified using a QIAquick 96 PCR fragment purification kit (Qiagen, Inc., Valencia, CA). .. The semi-automated fragment purification method uses the solutions provided in the kit and procedures modified to adapt to the FX instrument (Beckman Coulter, Inc.).

    Article Title: A Highly Reproducible, Linear, and Automated Sample Preparation Method for DNA Microarrays
    Article Snippet: .. All liquid and enzyme solution additions and purifications were performed on a QIAGEN BioRobot (model 9604 or 3000). cDNA purification and aRNA purification were performed using QIAquick 96 PCR purification kits and RNeasy96 kits (QIAGEN), respectively. ..

    Article Title: Extensive sequence variation in the 3′ untranslated region of the KRAS gene in lung and ovarian cancer cases
    Article Snippet: .. PCR products were purified using the QIAquick PCR purification kit, 96-PCR purification kit (Qiagen), or at the core sequencing laboratory at the W.M. ..

    Article Title: Population sequencing of two endocannabinoid metabolic genes identifies rare and common regulatory variants associated with extreme obesity and metabolite level
    Article Snippet: .. The fragmented DNA was purified in a Qiaquick 96 PCR purification plate (QIAGEN, Valencia, CA, USA) following the manufacturer's instructions. .. The DNA ends were repaired in a 100 μl reaction (1× NEB ligase buffer, 1 mg/ml bovine serum albumin, 200 μM dNTP) using 15 U T4 DNA polymerase (NEB Ipswich, MA, USA), 50 U T4 polynucleotide kinase (NEB), and 5 U Klenow DNA polymerase.

    Article Title: Genome wide copy number analysis of single cells
    Article Snippet: .. QIAquick 96 well purification kit (Qiagen, cat. no: 28181) . ..

    Article Title: A new sensitive PCR assay for one-step detection of 12 IDH1/2 mutations in glioma
    Article Snippet: .. PCR products were purified using the QIAquick 96 PCR Purification kit (Qiagen, Hilden). .. The purified PCR products (1.5 μL) were sequenced using Applied Biosystems Big Dye 3.1 dideoxy chain termination sequencing chemistry in conjunction with fluorescent-based capillary sequencers (Model 3730xl Applied Biosystems Division of Perkin Elmer, Foster City, California, USA) for electrophoresis, data collection and base calling.

    Sequencing:

    Article Title: Recent Acquisition of Helicobacter pylori by Baka Pygmies
    Article Snippet: PCR products were purified using the QIAquick 96 PCR Purification Kit (Qiagen). .. PCR amplicons were sequenced bidirectionally using the BigDye Terminator v1.1 Cycle Sequencing Kit and the 3130xl Genetic Analyzer (Applied Biosystems).

    Article Title: Genetic Diversity of Eurycoma longifolia Inferred from Single Nucleotide Polymorphisms 1 Inferred from Single Nucleotide Polymorphisms 1 [w]
    Article Snippet: Paragraph title: End Sequencing of Cloned DOP-PCR Products ... The entire products were loaded onto 1.1% (w/v) agarose gel, electrophoresed, and gel purified using Qiaquick 96 PCR purification kit (Qiagen USA).

    Article Title: Host Life History Strategy, Species Diversity, and Habitat Influence Trypanosoma cruzi Vector Infection in Changing Landscapes
    Article Snippet: PCR products were then purified with a high throughput adaptation of gel extraction followed by vacuum manifold PCR product purification using a QIAquick 96 PCR Purification Kit (Qiagen, Valencia, CA 91355) following manufacturer's instructions. .. After purification, PCR products were then tested for purity and DNA concentration with a Nanodrop spectrophotometer and sent for sequencing to the University of Georgia Bioinformatics laboratory.

    Article Title: Reduced-Median-Network Analysis of Complete Mitochondrial DNA Coding-Region Sequences for the Major African, Asian, and European Haplogroups
    Article Snippet: Triplicate reactions were pooled and purified with the QIAquick 96 PCR Purification Kit (Qiagen). .. Sequencing reactions were performed with 3 μl of PCR product, forward or reverse PCR primer, and BigDyeTerminator chemistry (PE Applied Biosystems).

    Article Title: Extensive sequence variation in the 3′ untranslated region of the KRAS gene in lung and ovarian cancer cases
    Article Snippet: .. PCR products were purified using the QIAquick PCR purification kit, 96-PCR purification kit (Qiagen), or at the core sequencing laboratory at the W.M. ..

    Article Title: A new sensitive PCR assay for one-step detection of 12 IDH1/2 mutations in glioma
    Article Snippet: Paragraph title: IDH1 and IDH2 sequencing ... PCR products were purified using the QIAquick 96 PCR Purification kit (Qiagen, Hilden).

    Gel Extraction:

    Article Title: Host Life History Strategy, Species Diversity, and Habitat Influence Trypanosoma cruzi Vector Infection in Changing Landscapes
    Article Snippet: .. PCR products were then purified with a high throughput adaptation of gel extraction followed by vacuum manifold PCR product purification using a QIAquick 96 PCR Purification Kit (Qiagen, Valencia, CA 91355) following manufacturer's instructions. .. After purification, PCR products were then tested for purity and DNA concentration with a Nanodrop spectrophotometer and sent for sequencing to the University of Georgia Bioinformatics laboratory.

    Plasmid Preparation:

    Article Title: High-throughput Protein Purification and Quality Assessment for Crystallization
    Article Snippet: Vector compatible primers are designed using an automated primer generator software that is part of the publicly available tools provided by the MCSG, where a list of target DNA sequences and the destination vectors is used as an input ( ) [ ]. .. After the thermocycler program is completed, the amplified fragments are purified using a QIAquick 96 PCR fragment purification kit (Qiagen, Inc., Valencia, CA).

    Software:

    Article Title: High-throughput Protein Purification and Quality Assessment for Crystallization
    Article Snippet: Vector compatible primers are designed using an automated primer generator software that is part of the publicly available tools provided by the MCSG, where a list of target DNA sequences and the destination vectors is used as an input ( ) [ ]. .. After the thermocycler program is completed, the amplified fragments are purified using a QIAquick 96 PCR fragment purification kit (Qiagen, Inc., Valencia, CA).

    Agarose Gel Electrophoresis:

    Article Title: Genetic Diversity of Eurycoma longifolia Inferred from Single Nucleotide Polymorphisms 1 Inferred from Single Nucleotide Polymorphisms 1 [w]
    Article Snippet: .. The entire products were loaded onto 1.1% (w/v) agarose gel, electrophoresed, and gel purified using Qiaquick 96 PCR purification kit (Qiagen USA). .. The purified PCR products were sent for automated sequencing at MWG Biotech Inc. (High Point, NC).

    Article Title: Host Life History Strategy, Species Diversity, and Habitat Influence Trypanosoma cruzi Vector Infection in Changing Landscapes
    Article Snippet: After the reactions, 1 l of PCR product was mixed with 5 l of loading dye and run on a 2% agarose gel that was stained with ethidium bromide in order to detect if the reaction was able to amplify vertebrate DNA in the bug. .. PCR products were then purified with a high throughput adaptation of gel extraction followed by vacuum manifold PCR product purification using a QIAquick 96 PCR Purification Kit (Qiagen, Valencia, CA 91355) following manufacturer's instructions.

    Next-Generation Sequencing:

    Article Title: Rapid Phenotypic and Genomic Change in Response to Therapeutic Pressure in Prostate Cancer Inferred by High Content Analysis of Single Circulating Tumor Cells
    Article Snippet: Paragraph title: Single Cell Next Generation Sequencing and Bioinformatic Analysis ... WGA was carried out manually in a 96-well plate format using the WGA4 Genomeplex Single Cell Whole Genome Amplification Kit (Sigma-Aldrich), followed by purification using a QIAquick 96 PCR Purification Kit (Qiagen).

    Spectrophotometry:

    Article Title: Host Life History Strategy, Species Diversity, and Habitat Influence Trypanosoma cruzi Vector Infection in Changing Landscapes
    Article Snippet: PCR products were then purified with a high throughput adaptation of gel extraction followed by vacuum manifold PCR product purification using a QIAquick 96 PCR Purification Kit (Qiagen, Valencia, CA 91355) following manufacturer's instructions. .. After purification, PCR products were then tested for purity and DNA concentration with a Nanodrop spectrophotometer and sent for sequencing to the University of Georgia Bioinformatics laboratory.

    Concentration Assay:

    Article Title: Host Life History Strategy, Species Diversity, and Habitat Influence Trypanosoma cruzi Vector Infection in Changing Landscapes
    Article Snippet: PCR products were then purified with a high throughput adaptation of gel extraction followed by vacuum manifold PCR product purification using a QIAquick 96 PCR Purification Kit (Qiagen, Valencia, CA 91355) following manufacturer's instructions. .. After purification, PCR products were then tested for purity and DNA concentration with a Nanodrop spectrophotometer and sent for sequencing to the University of Georgia Bioinformatics laboratory.

    Article Title: High-throughput Protein Purification and Quality Assessment for Crystallization
    Article Snippet: After the thermocycler program is completed, the amplified fragments are purified using a QIAquick 96 PCR fragment purification kit (Qiagen, Inc., Valencia, CA). .. The DNA concentration of the purified fragments is determined by a PicoGreen fluorescent assay (Invitrogen/Molecular Probes, Eugene, OR) using 96-well plates.

    Article Title: A new sensitive PCR assay for one-step detection of 12 IDH1/2 mutations in glioma
    Article Snippet: PCR products were purified using the QIAquick 96 PCR Purification kit (Qiagen, Hilden). .. Locked Nucleic Acid (LNA) Sanger sequencing was performed in a separate reaction by amplifying the mutation hotspot regions using the pyrosequencing PCR primers and LNA (+T + A + G + G + T + C + G + T + C + A+/3InvdT/) at the final concentration of 70 nM.

    High Throughput Screening Assay:

    Article Title: Host Life History Strategy, Species Diversity, and Habitat Influence Trypanosoma cruzi Vector Infection in Changing Landscapes
    Article Snippet: .. PCR products were then purified with a high throughput adaptation of gel extraction followed by vacuum manifold PCR product purification using a QIAquick 96 PCR Purification Kit (Qiagen, Valencia, CA 91355) following manufacturer's instructions. .. After purification, PCR products were then tested for purity and DNA concentration with a Nanodrop spectrophotometer and sent for sequencing to the University of Georgia Bioinformatics laboratory.

    Staining:

    Article Title: Host Life History Strategy, Species Diversity, and Habitat Influence Trypanosoma cruzi Vector Infection in Changing Landscapes
    Article Snippet: After the reactions, 1 l of PCR product was mixed with 5 l of loading dye and run on a 2% agarose gel that was stained with ethidium bromide in order to detect if the reaction was able to amplify vertebrate DNA in the bug. .. PCR products were then purified with a high throughput adaptation of gel extraction followed by vacuum manifold PCR product purification using a QIAquick 96 PCR Purification Kit (Qiagen, Valencia, CA 91355) following manufacturer's instructions.

    Variant Assay:

    Article Title: Extensive sequence variation in the 3′ untranslated region of the KRAS gene in lung and ovarian cancer cases
    Article Snippet: PCR products were purified using the QIAquick PCR purification kit, 96-PCR purification kit (Qiagen), or at the core sequencing laboratory at the W.M. .. Sequences of the each segment were aligned to the annotated 3′ UTR of the KRAS transcript variant B from NCBI36/hg18 refseq ( , chr12: 25249447–25253995) using the SeqMan program (DNA Star).

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  • 99
    Qiagen qiaquick 96 pcr purification plate
    Qiaquick 96 Pcr Purification Plate, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaquick 96 pcr purification plate/product/Qiagen
    Average 99 stars, based on 119 article reviews
    Price from $9.99 to $1999.99
    qiaquick 96 pcr purification plate - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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