qiaprep spin miniprep kit  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Thermo Fisher qiaprep spin miniprep kit
    Qiaprep Spin Miniprep Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaprep spin miniprep kit/product/Thermo Fisher
    Average 96 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    qiaprep spin miniprep kit - by Bioz Stars, 2020-03
    96/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Discovering Protein-Protein Interactions Using Nucleic Acid Programmable Protein Arrays
    Article Snippet: Miniprep the query gene using the QIAprep Spin Miniprep kit and quantify DNA concentration with the NanoDrop Spectrophotometer. .. While both DNA miniprep and maxiprep are able to produce DNA of good quality as well as adequate concentration for sequencing or cloning purpose, the former is more time-efficient and can be done for a relatively large amount of DNA samples each time.

    Article Title: Functional Analyses of Transcription Factor Binding Sites that Differ between Present-Day and Archaic Humans
    Article Snippet: .. DNA was extracted from individual clones using the Qiaprep Spin Miniprep Kit and colony PCR was performed (Thermo Scientific Phusion polymerase, annealing temperature 60 °C, primers TTT TTG TGT GAA TCG ATA GTA CTA ACA TAC GCT and GAG CTT CCA TTA TAT ACC CTC TAG TGT CTA AGC for regulatory regions, primers AGA TTC TCA TTA AGG CCA AGA AGG G and TCA CTG CAT TCT AGT TGT GGT TTG TCC for tag region). .. Plasmids that matched expected insert sizes for both amplifications were used to construct Illumina sequencing libraries ( ) and fully sequenced on an Illumina MiSeq (paired end 2 × (150 bp + 7 bp)) for sequence verification and association of the tags with TFBSs.

    Article Title: Sea Star Wasting Disease in Asterias forbesi along the Atlantic Coast of North America
    Article Snippet: Paragraph title: Amplification, cloning and sequencing of SSaDV DNA from A . forbesi ... Plasmid DNA was isolated using a QiaPrep Spin MiniPrep kit and DNA concentration (μg/μL) was quantified using the Thermo Scientific Nanodrop ND8000 version 2.2.1 system.

    Article Title: The Effects of 4-Hydroxybenzoic Acid Identified from Bamboo (Dendrocalamus asper) Shoots on Kv1.4 Channel
    Article Snippet: Paragraph title: cDNA Cloning and in vitro Transcription ... QIAprep Spin Miniprep Kit (Cat. No. 27104) was used for purification of the plasmid cDNA, followed by determination of cDNA concentration using spectrophotometer NanoDrop 2000 (ThermoScientificTM , Massachusetts, USA).

    Article Title: Leptin in Whales: Validation and Measurement of mRNA Expression by Absolute Quantitative Real-Time PCR
    Article Snippet: Positive results for each gene were cloned into pCR®4-TOPO® vector using a TOPO TA Cloning® kit (Invitrogen™) following manufacturer recommended protocols and plated on X-Gal-treated LB/AMP plates. .. Colonies were PCR checked for correct insertion and plasmid preparations completed using QIAprep® Spin Miniprep Kit (Invitrogen™).

    Article Title: Molecular Characterization of the Nonphotosynthetic Partner Bacterium in the Consortium "Chlorochromatium aggregatum"
    Article Snippet: Paragraph title: Cloning. ... Plasmids were extracted with a QIAprep spin miniprep kit (Invitrogen), and the presence of inserts was verified by digestion with EcoRI, PvuI, and HaeIII (MBI Fermentas, St. Leon-Rot, Germany).

    Article Title: Development of an Unbiased Antigen-Mining Approach To Identify Novel Vaccine Antigens and Diagnostic Reagents for Bovine Tuberculosis
    Article Snippet: .. DH5α transformants containing the petDest42 expression clones were selected by growth on ampicillin-supplemented LB+G (LB+G/amp+ ) agar plates and cultured overnight at 37°C in LB+G/amp+ broth to increase the yield of the expression clone, which was subsequently isolated using the QIAprep Spin miniprep kit and used to transform BL21(DE3) cells (Invitrogen). .. Similarly, BL21(DE3) cells containing the expression clone were selected and expanded by overnight culture, and protein expression was induced using Overnight Express Autoinduction System 1 (Novagen, United Kingdom), following the manufacturer's protocol.

    Centrifugation:

    Article Title: Development of an Unbiased Antigen-Mining Approach To Identify Novel Vaccine Antigens and Diagnostic Reagents for Bovine Tuberculosis
    Article Snippet: DH5α transformants containing the petDest42 expression clones were selected by growth on ampicillin-supplemented LB+G (LB+G/amp+ ) agar plates and cultured overnight at 37°C in LB+G/amp+ broth to increase the yield of the expression clone, which was subsequently isolated using the QIAprep Spin miniprep kit and used to transform BL21(DE3) cells (Invitrogen). .. Induced BL21(DE3) cells were pelleted by centrifugation, resuspended in binding buffer (8 M urea, 20 mM Tris-HCl, 500 mM NaCl, 50 mM imidazole, 1 mM β-mercaptoethanol [pH 7.4]), and mechanically disrupted using a FastPrep homogenizer (Thermo, United Kingdom) following the addition of lysis matrix.

    Amplification:

    Article Title: Sea Star Wasting Disease in Asterias forbesi along the Atlantic Coast of North America
    Article Snippet: Paragraph title: Amplification, cloning and sequencing of SSaDV DNA from A . forbesi ... Plasmid DNA was isolated using a QiaPrep Spin MiniPrep kit and DNA concentration (μg/μL) was quantified using the Thermo Scientific Nanodrop ND8000 version 2.2.1 system.

    Article Title: Targeted Mutation of NGN3 Gene Disrupts Pancreatic Endocrine Cell Development in Pigs
    Article Snippet: .. Plasmids were isolated from five to fifteen colonies using QIAprep spin miniprep kit, and DNA concentration determined using a NanoDrop ND-1000 spectrophotometer, followed by EcoRI restriction digest to confirm amplicon insertion. .. An average of 10 colonies were used to confirm genotypes from both the ear and tail PCR amplicons.

    Article Title: Leptin in Whales: Validation and Measurement of mRNA Expression by Absolute Quantitative Real-Time PCR
    Article Snippet: PCR amplification of leptin and OB-RGRP products were done in an Eppendorf Personal minicycler (Eppendorf) under the following conditions: initial denaturation at 94°C for 2 minutes; 30 cycles of 94°C for 30s, 55°C for 30s, 70°C for 2 minutes and final extension at 72°C for 5 minutes. .. Colonies were PCR checked for correct insertion and plasmid preparations completed using QIAprep® Spin Miniprep Kit (Invitrogen™).

    Synthesized:

    Article Title: Functional Analyses of Transcription Factor Binding Sites that Differ between Present-Day and Archaic Humans
    Article Snippet: We synthesized a single-stranded oligonucleotide of 20 random bases (AAC CAT AGG ATT CGC GTC AGT CTA GAT AGN NNN NNN NNN NNN NNN NNN NGG CCG GCC GTC TGG CGT CTT GGC TAA TC) flanked on the 5’-end by an Xba I site and the sequence “TAG” and an Fse I site on the 3’-end (Invitrogen) as well as primer sites used to make the oligo double stranded by two cycles of polymerase chain reaction (PCR) (Thermo Scientific Phusion polymerase, annealing temperature 60 °C, primers AAC CAT AGG ATT CGC GTC AG and GAT TAG CCA AGA CGC CAG AC). .. DNA was extracted from individual clones using the Qiaprep Spin Miniprep Kit and colony PCR was performed (Thermo Scientific Phusion polymerase, annealing temperature 60 °C, primers TTT TTG TGT GAA TCG ATA GTA CTA ACA TAC GCT and GAG CTT CCA TTA TAT ACC CTC TAG TGT CTA AGC for regulatory regions, primers AGA TTC TCA TTA AGG CCA AGA AGG G and TCA CTG CAT TCT AGT TGT GGT TTG TCC for tag region).

    TA Cloning:

    Article Title: Leptin in Whales: Validation and Measurement of mRNA Expression by Absolute Quantitative Real-Time PCR
    Article Snippet: Positive results for each gene were cloned into pCR®4-TOPO® vector using a TOPO TA Cloning® kit (Invitrogen™) following manufacturer recommended protocols and plated on X-Gal-treated LB/AMP plates. .. Colonies were PCR checked for correct insertion and plasmid preparations completed using QIAprep® Spin Miniprep Kit (Invitrogen™).

    Article Title: Molecular Characterization of the Nonphotosynthetic Partner Bacterium in the Consortium "Chlorochromatium aggregatum"
    Article Snippet: PCR products were cloned through chemical transformation with a TOPO TA cloning kit (version P; Invitrogen, Carlsbad, CA). .. Plasmids were extracted with a QIAprep spin miniprep kit (Invitrogen), and the presence of inserts was verified by digestion with EcoRI, PvuI, and HaeIII (MBI Fermentas, St. Leon-Rot, Germany).

    Article Title: Distinctive G Protein-Dependent Signaling by Protease-Activated Receptor 2 (PAR2) in Smooth Muscle: Feedback Inhibition of RhoA by cAMP-Independent PKA
    Article Snippet: .. Materials and Methods PAR2-activating peptide (PAR2-AP, SLIGRL) was obtained from Bachem, Torrance, CA; [125 I]cAMP, [γ-32 P]ATP, [32 P]Pi, [35 S]GTPγS, and [3 H]myo-inositol were obtained from Perkin Elmer Life Sciences, Boston, MA; Collagenase CLS type II and soybean trypsin inhibitor were obtained from Worthington, Freehold, NJ; Western blotting, Dowex AG-1×8 resin (100–200 mesh in formate form), chromatography material and protein assay kit from Bio-Rad Laboratories, Hercules, CA; antibodies to Gaq , Gai1 , Gai2 , Gai3 , Ga12 , Ga13 , Gas , phospho-specific MYPT1 (Thr696 ), MLC20 (Ser19 ) and CPI-17 (Thr38 ), Rho kinase, PKA catalytic subunit, IKK2(ser177/181 ), IkBa, and p65 subunit were from Santa Cruz Biotechnology, Santa Cruz, CA; myelin basic protein (MBP) was from Upstate Biotechnology; ML-9 was from Biomol Research Laboratories, Plymouth Meeting, PA; bisindolylmaleimide, Y27632, pertussis toxin, cAMP; Clostridium botulinum C3 exoenzyme, IKK-2 Inhibitor IV (IKK IV), MG-132, myristoylated PKI and U73122 were from Calbiochem, La Jolla, CA; RNAqueous™ kit was from Ambion, Austin, TX; Effectene Transfection Reagent, QIAEX®II Gel extraction Kit and QIAprep®Spin Miniprep Kit were from Germantown, MD; PCR reagents were from Applied Biosystems, Roche; SuperScript™ II Reverse Transcriptese and TOPO TA Cloning® Kit Dual Promoter were from Invitrogen, Grand Island, NY; EcoR I was from New England Bio Labs, Ipswich, MA; All other chemicals were from Sigma, St. Louis, MO. ..

    Construct:

    Article Title: Discovering Protein-Protein Interactions Using Nucleic Acid Programmable Protein Arrays
    Article Snippet: Paragraph title: Construct the query gene expression vector ... Miniprep the query gene using the QIAprep Spin Miniprep kit and quantify DNA concentration with the NanoDrop Spectrophotometer.

    Article Title: Functional Analyses of Transcription Factor Binding Sites that Differ between Present-Day and Archaic Humans
    Article Snippet: DNA was extracted from individual clones using the Qiaprep Spin Miniprep Kit and colony PCR was performed (Thermo Scientific Phusion polymerase, annealing temperature 60 °C, primers TTT TTG TGT GAA TCG ATA GTA CTA ACA TAC GCT and GAG CTT CCA TTA TAT ACC CTC TAG TGT CTA AGC for regulatory regions, primers AGA TTC TCA TTA AGG CCA AGA AGG G and TCA CTG CAT TCT AGT TGT GGT TTG TCC for tag region). .. Plasmids that matched expected insert sizes for both amplifications were used to construct Illumina sequencing libraries ( ) and fully sequenced on an Illumina MiSeq (paired end 2 × (150 bp + 7 bp)) for sequence verification and association of the tags with TFBSs.

    SYBR Green Assay:

    Article Title: Leptin in Whales: Validation and Measurement of mRNA Expression by Absolute Quantitative Real-Time PCR
    Article Snippet: Colonies were PCR checked for correct insertion and plasmid preparations completed using QIAprep® Spin Miniprep Kit (Invitrogen™). .. Relative qPCR assays were performed with iTaq™ SYBR® green Supermix with ROX (Biorad).

    Expressing:

    Article Title: Discovering Protein-Protein Interactions Using Nucleic Acid Programmable Protein Arrays
    Article Snippet: Paragraph title: Construct the query gene expression vector ... Miniprep the query gene using the QIAprep Spin Miniprep kit and quantify DNA concentration with the NanoDrop Spectrophotometer.

    Article Title: Leptin in Whales: Validation and Measurement of mRNA Expression by Absolute Quantitative Real-Time PCR
    Article Snippet: Paragraph title: Relative Expression qPCR Assays ... Colonies were PCR checked for correct insertion and plasmid preparations completed using QIAprep® Spin Miniprep Kit (Invitrogen™).

    Article Title: Development of an Unbiased Antigen-Mining Approach To Identify Novel Vaccine Antigens and Diagnostic Reagents for Bovine Tuberculosis
    Article Snippet: .. DH5α transformants containing the petDest42 expression clones were selected by growth on ampicillin-supplemented LB+G (LB+G/amp+ ) agar plates and cultured overnight at 37°C in LB+G/amp+ broth to increase the yield of the expression clone, which was subsequently isolated using the QIAprep Spin miniprep kit and used to transform BL21(DE3) cells (Invitrogen). .. Similarly, BL21(DE3) cells containing the expression clone were selected and expanded by overnight culture, and protein expression was induced using Overnight Express Autoinduction System 1 (Novagen, United Kingdom), following the manufacturer's protocol.

    Western Blot:

    Article Title: Distinctive G Protein-Dependent Signaling by Protease-Activated Receptor 2 (PAR2) in Smooth Muscle: Feedback Inhibition of RhoA by cAMP-Independent PKA
    Article Snippet: .. Materials and Methods PAR2-activating peptide (PAR2-AP, SLIGRL) was obtained from Bachem, Torrance, CA; [125 I]cAMP, [γ-32 P]ATP, [32 P]Pi, [35 S]GTPγS, and [3 H]myo-inositol were obtained from Perkin Elmer Life Sciences, Boston, MA; Collagenase CLS type II and soybean trypsin inhibitor were obtained from Worthington, Freehold, NJ; Western blotting, Dowex AG-1×8 resin (100–200 mesh in formate form), chromatography material and protein assay kit from Bio-Rad Laboratories, Hercules, CA; antibodies to Gaq , Gai1 , Gai2 , Gai3 , Ga12 , Ga13 , Gas , phospho-specific MYPT1 (Thr696 ), MLC20 (Ser19 ) and CPI-17 (Thr38 ), Rho kinase, PKA catalytic subunit, IKK2(ser177/181 ), IkBa, and p65 subunit were from Santa Cruz Biotechnology, Santa Cruz, CA; myelin basic protein (MBP) was from Upstate Biotechnology; ML-9 was from Biomol Research Laboratories, Plymouth Meeting, PA; bisindolylmaleimide, Y27632, pertussis toxin, cAMP; Clostridium botulinum C3 exoenzyme, IKK-2 Inhibitor IV (IKK IV), MG-132, myristoylated PKI and U73122 were from Calbiochem, La Jolla, CA; RNAqueous™ kit was from Ambion, Austin, TX; Effectene Transfection Reagent, QIAEX®II Gel extraction Kit and QIAprep®Spin Miniprep Kit were from Germantown, MD; PCR reagents were from Applied Biosystems, Roche; SuperScript™ II Reverse Transcriptese and TOPO TA Cloning® Kit Dual Promoter were from Invitrogen, Grand Island, NY; EcoR I was from New England Bio Labs, Ipswich, MA; All other chemicals were from Sigma, St. Louis, MO. ..

    Transformation Assay:

    Article Title: Functional Analyses of Transcription Factor Binding Sites that Differ between Present-Day and Archaic Humans
    Article Snippet: The TFBSs as well as a CMV enhancer (GenBank: U02451.1; nucleotides 18–431) were cloned into the pGL4.23 plasmid (Promega) and grown in E s cherichia coli DH5alpha (New England Biolabs) and clones from each transformation were pooled and DNA extracted using the Qiaprep Spin Miniprep Kit. .. DNA was extracted from individual clones using the Qiaprep Spin Miniprep Kit and colony PCR was performed (Thermo Scientific Phusion polymerase, annealing temperature 60 °C, primers TTT TTG TGT GAA TCG ATA GTA CTA ACA TAC GCT and GAG CTT CCA TTA TAT ACC CTC TAG TGT CTA AGC for regulatory regions, primers AGA TTC TCA TTA AGG CCA AGA AGG G and TCA CTG CAT TCT AGT TGT GGT TTG TCC for tag region).

    Article Title: Targeted Mutation of NGN3 Gene Disrupts Pancreatic Endocrine Cell Development in Pigs
    Article Snippet: Products were purified over QIAprep DNA miniprep columns, subject to A-tailing and ligated into pCR™2.1 vector, and transformed into DH5α electrocompetent cells (prepared in-house). .. Plasmids were isolated from five to fifteen colonies using QIAprep spin miniprep kit, and DNA concentration determined using a NanoDrop ND-1000 spectrophotometer, followed by EcoRI restriction digest to confirm amplicon insertion.

    Article Title: Molecular Characterization of the Nonphotosynthetic Partner Bacterium in the Consortium "Chlorochromatium aggregatum"
    Article Snippet: PCR products were cloned through chemical transformation with a TOPO TA cloning kit (version P; Invitrogen, Carlsbad, CA). .. Plasmids were extracted with a QIAprep spin miniprep kit (Invitrogen), and the presence of inserts was verified by digestion with EcoRI, PvuI, and HaeIII (MBI Fermentas, St. Leon-Rot, Germany).

    Transfection:

    Article Title: Distinctive G Protein-Dependent Signaling by Protease-Activated Receptor 2 (PAR2) in Smooth Muscle: Feedback Inhibition of RhoA by cAMP-Independent PKA
    Article Snippet: .. Materials and Methods PAR2-activating peptide (PAR2-AP, SLIGRL) was obtained from Bachem, Torrance, CA; [125 I]cAMP, [γ-32 P]ATP, [32 P]Pi, [35 S]GTPγS, and [3 H]myo-inositol were obtained from Perkin Elmer Life Sciences, Boston, MA; Collagenase CLS type II and soybean trypsin inhibitor were obtained from Worthington, Freehold, NJ; Western blotting, Dowex AG-1×8 resin (100–200 mesh in formate form), chromatography material and protein assay kit from Bio-Rad Laboratories, Hercules, CA; antibodies to Gaq , Gai1 , Gai2 , Gai3 , Ga12 , Ga13 , Gas , phospho-specific MYPT1 (Thr696 ), MLC20 (Ser19 ) and CPI-17 (Thr38 ), Rho kinase, PKA catalytic subunit, IKK2(ser177/181 ), IkBa, and p65 subunit were from Santa Cruz Biotechnology, Santa Cruz, CA; myelin basic protein (MBP) was from Upstate Biotechnology; ML-9 was from Biomol Research Laboratories, Plymouth Meeting, PA; bisindolylmaleimide, Y27632, pertussis toxin, cAMP; Clostridium botulinum C3 exoenzyme, IKK-2 Inhibitor IV (IKK IV), MG-132, myristoylated PKI and U73122 were from Calbiochem, La Jolla, CA; RNAqueous™ kit was from Ambion, Austin, TX; Effectene Transfection Reagent, QIAEX®II Gel extraction Kit and QIAprep®Spin Miniprep Kit were from Germantown, MD; PCR reagents were from Applied Biosystems, Roche; SuperScript™ II Reverse Transcriptese and TOPO TA Cloning® Kit Dual Promoter were from Invitrogen, Grand Island, NY; EcoR I was from New England Bio Labs, Ipswich, MA; All other chemicals were from Sigma, St. Louis, MO. ..

    Chromatography:

    Article Title: Distinctive G Protein-Dependent Signaling by Protease-Activated Receptor 2 (PAR2) in Smooth Muscle: Feedback Inhibition of RhoA by cAMP-Independent PKA
    Article Snippet: .. Materials and Methods PAR2-activating peptide (PAR2-AP, SLIGRL) was obtained from Bachem, Torrance, CA; [125 I]cAMP, [γ-32 P]ATP, [32 P]Pi, [35 S]GTPγS, and [3 H]myo-inositol were obtained from Perkin Elmer Life Sciences, Boston, MA; Collagenase CLS type II and soybean trypsin inhibitor were obtained from Worthington, Freehold, NJ; Western blotting, Dowex AG-1×8 resin (100–200 mesh in formate form), chromatography material and protein assay kit from Bio-Rad Laboratories, Hercules, CA; antibodies to Gaq , Gai1 , Gai2 , Gai3 , Ga12 , Ga13 , Gas , phospho-specific MYPT1 (Thr696 ), MLC20 (Ser19 ) and CPI-17 (Thr38 ), Rho kinase, PKA catalytic subunit, IKK2(ser177/181 ), IkBa, and p65 subunit were from Santa Cruz Biotechnology, Santa Cruz, CA; myelin basic protein (MBP) was from Upstate Biotechnology; ML-9 was from Biomol Research Laboratories, Plymouth Meeting, PA; bisindolylmaleimide, Y27632, pertussis toxin, cAMP; Clostridium botulinum C3 exoenzyme, IKK-2 Inhibitor IV (IKK IV), MG-132, myristoylated PKI and U73122 were from Calbiochem, La Jolla, CA; RNAqueous™ kit was from Ambion, Austin, TX; Effectene Transfection Reagent, QIAEX®II Gel extraction Kit and QIAprep®Spin Miniprep Kit were from Germantown, MD; PCR reagents were from Applied Biosystems, Roche; SuperScript™ II Reverse Transcriptese and TOPO TA Cloning® Kit Dual Promoter were from Invitrogen, Grand Island, NY; EcoR I was from New England Bio Labs, Ipswich, MA; All other chemicals were from Sigma, St. Louis, MO. ..

    Transferring:

    Article Title: Discovering Protein-Protein Interactions Using Nucleic Acid Programmable Protein Arrays
    Article Snippet: Pick single colonies with pipette tips, inoculate in 5 mL of LB medium supplemented with 5 μL of 100 mg/mL ampicillin, and culture overnight at 250 rpm at 37 °C in a ground shaker. .. Miniprep the query gene using the QIAprep Spin Miniprep kit and quantify DNA concentration with the NanoDrop Spectrophotometer.

    Cell Culture:

    Article Title: Development of an Unbiased Antigen-Mining Approach To Identify Novel Vaccine Antigens and Diagnostic Reagents for Bovine Tuberculosis
    Article Snippet: .. DH5α transformants containing the petDest42 expression clones were selected by growth on ampicillin-supplemented LB+G (LB+G/amp+ ) agar plates and cultured overnight at 37°C in LB+G/amp+ broth to increase the yield of the expression clone, which was subsequently isolated using the QIAprep Spin miniprep kit and used to transform BL21(DE3) cells (Invitrogen). .. Similarly, BL21(DE3) cells containing the expression clone were selected and expanded by overnight culture, and protein expression was induced using Overnight Express Autoinduction System 1 (Novagen, United Kingdom), following the manufacturer's protocol.

    Polymerase Chain Reaction:

    Article Title: Functional Analyses of Transcription Factor Binding Sites that Differ between Present-Day and Archaic Humans
    Article Snippet: .. DNA was extracted from individual clones using the Qiaprep Spin Miniprep Kit and colony PCR was performed (Thermo Scientific Phusion polymerase, annealing temperature 60 °C, primers TTT TTG TGT GAA TCG ATA GTA CTA ACA TAC GCT and GAG CTT CCA TTA TAT ACC CTC TAG TGT CTA AGC for regulatory regions, primers AGA TTC TCA TTA AGG CCA AGA AGG G and TCA CTG CAT TCT AGT TGT GGT TTG TCC for tag region). .. Plasmids that matched expected insert sizes for both amplifications were used to construct Illumina sequencing libraries ( ) and fully sequenced on an Illumina MiSeq (paired end 2 × (150 bp + 7 bp)) for sequence verification and association of the tags with TFBSs.

    Article Title: Targeted Mutation of NGN3 Gene Disrupts Pancreatic Endocrine Cell Development in Pigs
    Article Snippet: Products were purified over QIAprep DNA miniprep columns, subject to A-tailing and ligated into pCR™2.1 vector, and transformed into DH5α electrocompetent cells (prepared in-house). .. Plasmids were isolated from five to fifteen colonies using QIAprep spin miniprep kit, and DNA concentration determined using a NanoDrop ND-1000 spectrophotometer, followed by EcoRI restriction digest to confirm amplicon insertion.

    Article Title: Leptin in Whales: Validation and Measurement of mRNA Expression by Absolute Quantitative Real-Time PCR
    Article Snippet: .. Colonies were PCR checked for correct insertion and plasmid preparations completed using QIAprep® Spin Miniprep Kit (Invitrogen™). .. Plasmid preparations were quantified via Nanodrop® spectrophotometer as previously described and sequenced using BigDye® Terminator v3.1 Cycle Sequencing kit and an ABI 3130xl genetic analyzer (Applied Biosystems, Foster City, CA) to confirm sequence identities prior to real-time PCR assays.

    Article Title: Molecular Characterization of the Nonphotosynthetic Partner Bacterium in the Consortium "Chlorochromatium aggregatum"
    Article Snippet: PCR products were cloned through chemical transformation with a TOPO TA cloning kit (version P; Invitrogen, Carlsbad, CA). .. Plasmids were extracted with a QIAprep spin miniprep kit (Invitrogen), and the presence of inserts was verified by digestion with EcoRI, PvuI, and HaeIII (MBI Fermentas, St. Leon-Rot, Germany).

    Article Title: Distinctive G Protein-Dependent Signaling by Protease-Activated Receptor 2 (PAR2) in Smooth Muscle: Feedback Inhibition of RhoA by cAMP-Independent PKA
    Article Snippet: .. Materials and Methods PAR2-activating peptide (PAR2-AP, SLIGRL) was obtained from Bachem, Torrance, CA; [125 I]cAMP, [γ-32 P]ATP, [32 P]Pi, [35 S]GTPγS, and [3 H]myo-inositol were obtained from Perkin Elmer Life Sciences, Boston, MA; Collagenase CLS type II and soybean trypsin inhibitor were obtained from Worthington, Freehold, NJ; Western blotting, Dowex AG-1×8 resin (100–200 mesh in formate form), chromatography material and protein assay kit from Bio-Rad Laboratories, Hercules, CA; antibodies to Gaq , Gai1 , Gai2 , Gai3 , Ga12 , Ga13 , Gas , phospho-specific MYPT1 (Thr696 ), MLC20 (Ser19 ) and CPI-17 (Thr38 ), Rho kinase, PKA catalytic subunit, IKK2(ser177/181 ), IkBa, and p65 subunit were from Santa Cruz Biotechnology, Santa Cruz, CA; myelin basic protein (MBP) was from Upstate Biotechnology; ML-9 was from Biomol Research Laboratories, Plymouth Meeting, PA; bisindolylmaleimide, Y27632, pertussis toxin, cAMP; Clostridium botulinum C3 exoenzyme, IKK-2 Inhibitor IV (IKK IV), MG-132, myristoylated PKI and U73122 were from Calbiochem, La Jolla, CA; RNAqueous™ kit was from Ambion, Austin, TX; Effectene Transfection Reagent, QIAEX®II Gel extraction Kit and QIAprep®Spin Miniprep Kit were from Germantown, MD; PCR reagents were from Applied Biosystems, Roche; SuperScript™ II Reverse Transcriptese and TOPO TA Cloning® Kit Dual Promoter were from Invitrogen, Grand Island, NY; EcoR I was from New England Bio Labs, Ipswich, MA; All other chemicals were from Sigma, St. Louis, MO. ..

    Binding Assay:

    Article Title: Development of an Unbiased Antigen-Mining Approach To Identify Novel Vaccine Antigens and Diagnostic Reagents for Bovine Tuberculosis
    Article Snippet: DH5α transformants containing the petDest42 expression clones were selected by growth on ampicillin-supplemented LB+G (LB+G/amp+ ) agar plates and cultured overnight at 37°C in LB+G/amp+ broth to increase the yield of the expression clone, which was subsequently isolated using the QIAprep Spin miniprep kit and used to transform BL21(DE3) cells (Invitrogen). .. Induced BL21(DE3) cells were pelleted by centrifugation, resuspended in binding buffer (8 M urea, 20 mM Tris-HCl, 500 mM NaCl, 50 mM imidazole, 1 mM β-mercaptoethanol [pH 7.4]), and mechanically disrupted using a FastPrep homogenizer (Thermo, United Kingdom) following the addition of lysis matrix.

    Staining:

    Article Title: Sea Star Wasting Disease in Asterias forbesi along the Atlantic Coast of North America
    Article Snippet: Samples were run through a Mastercycler® Nexus X2 (Eppendorf) with the following cycling conditions: 94°C for 10 min, then 35 cycles of 94°C for 30 s, 50°C for 30 s, 72°C for 90 s, with a final extension at 72°C for 5 min. Amplicons were run on a 1% agarose gel in TAE buffer (40 mM Tris, pH = 7.6, 20 mM acetic acid, 1 mM EDTA) for 30 min at 100 V, stained, destained and bands were visualized using the BioRad Quantity 1 System. .. Plasmid DNA was isolated using a QiaPrep Spin MiniPrep kit and DNA concentration (μg/μL) was quantified using the Thermo Scientific Nanodrop ND8000 version 2.2.1 system.

    Article Title: Leptin in Whales: Validation and Measurement of mRNA Expression by Absolute Quantitative Real-Time PCR
    Article Snippet: All PCR results were evaluated with gel electrophoresis with subsequent ethidium bromide staining. .. Colonies were PCR checked for correct insertion and plasmid preparations completed using QIAprep® Spin Miniprep Kit (Invitrogen™).

    DNA Extraction:

    Article Title: Targeted Mutation of NGN3 Gene Disrupts Pancreatic Endocrine Cell Development in Pigs
    Article Snippet: Paragraph title: Genomic DNA isolation and genotyping of edited embryos, fetuses and animals ... Plasmids were isolated from five to fifteen colonies using QIAprep spin miniprep kit, and DNA concentration determined using a NanoDrop ND-1000 spectrophotometer, followed by EcoRI restriction digest to confirm amplicon insertion.

    Nucleic Acid Electrophoresis:

    Article Title: Leptin in Whales: Validation and Measurement of mRNA Expression by Absolute Quantitative Real-Time PCR
    Article Snippet: All PCR results were evaluated with gel electrophoresis with subsequent ethidium bromide staining. .. Colonies were PCR checked for correct insertion and plasmid preparations completed using QIAprep® Spin Miniprep Kit (Invitrogen™).

    Real-time Polymerase Chain Reaction:

    Article Title: Leptin in Whales: Validation and Measurement of mRNA Expression by Absolute Quantitative Real-Time PCR
    Article Snippet: Paragraph title: Relative Expression qPCR Assays ... Colonies were PCR checked for correct insertion and plasmid preparations completed using QIAprep® Spin Miniprep Kit (Invitrogen™).

    Isolation:

    Article Title: Sea Star Wasting Disease in Asterias forbesi along the Atlantic Coast of North America
    Article Snippet: .. Plasmid DNA was isolated using a QiaPrep Spin MiniPrep kit and DNA concentration (μg/μL) was quantified using the Thermo Scientific Nanodrop ND8000 version 2.2.1 system. .. Samples were prepared for sequencing following standard procedure for the RI Genomics and Sequencing Center at the University of Rhode Island [ http://web.uri.edu/gsc/ ].

    Article Title: Targeted Mutation of NGN3 Gene Disrupts Pancreatic Endocrine Cell Development in Pigs
    Article Snippet: .. Plasmids were isolated from five to fifteen colonies using QIAprep spin miniprep kit, and DNA concentration determined using a NanoDrop ND-1000 spectrophotometer, followed by EcoRI restriction digest to confirm amplicon insertion. .. An average of 10 colonies were used to confirm genotypes from both the ear and tail PCR amplicons.

    Article Title: The Effects of 4-Hydroxybenzoic Acid Identified from Bamboo (Dendrocalamus asper) Shoots on Kv1.4 Channel
    Article Snippet: QIAprep Spin Miniprep Kit (Cat. No. 27104) was used for purification of the plasmid cDNA, followed by determination of cDNA concentration using spectrophotometer NanoDrop 2000 (ThermoScientificTM , Massachusetts, USA). .. The cRNA was isolated and purified with RNeasy Mini Kit (Cat. No. 74104) and the concentration of the cRNA was determined using NanoDrop 2000.

    Article Title: Development of an Unbiased Antigen-Mining Approach To Identify Novel Vaccine Antigens and Diagnostic Reagents for Bovine Tuberculosis
    Article Snippet: .. DH5α transformants containing the petDest42 expression clones were selected by growth on ampicillin-supplemented LB+G (LB+G/amp+ ) agar plates and cultured overnight at 37°C in LB+G/amp+ broth to increase the yield of the expression clone, which was subsequently isolated using the QIAprep Spin miniprep kit and used to transform BL21(DE3) cells (Invitrogen). .. Similarly, BL21(DE3) cells containing the expression clone were selected and expanded by overnight culture, and protein expression was induced using Overnight Express Autoinduction System 1 (Novagen, United Kingdom), following the manufacturer's protocol.

    Purification:

    Article Title: Sea Star Wasting Disease in Asterias forbesi along the Atlantic Coast of North America
    Article Snippet: Amplicon DNA was purified from the gel and cloned using the pGEM-T Easy Vector System 1 method (Promega) following the manufacturer’s protocol. .. Plasmid DNA was isolated using a QiaPrep Spin MiniPrep kit and DNA concentration (μg/μL) was quantified using the Thermo Scientific Nanodrop ND8000 version 2.2.1 system.

    Article Title: Targeted Mutation of NGN3 Gene Disrupts Pancreatic Endocrine Cell Development in Pigs
    Article Snippet: Products were purified over QIAprep DNA miniprep columns, subject to A-tailing and ligated into pCR™2.1 vector, and transformed into DH5α electrocompetent cells (prepared in-house). .. Plasmids were isolated from five to fifteen colonies using QIAprep spin miniprep kit, and DNA concentration determined using a NanoDrop ND-1000 spectrophotometer, followed by EcoRI restriction digest to confirm amplicon insertion.

    Article Title: Isoferulic acid prevents methylglyoxal-induced protein glycation and DNA damage by free radical scavenging activity
    Article Snippet: .. Plasmid extraction The pUC19 plasmid was purified from competent Escherichia coli by using QIAprep Spin Miniprep kit according to the manufacturer’s protocol and measured DNA concentration using the NanoDrop-1000 spectrophotometer (Thermo Scientific, MA, USA). ..

    Article Title: The Effects of 4-Hydroxybenzoic Acid Identified from Bamboo (Dendrocalamus asper) Shoots on Kv1.4 Channel
    Article Snippet: .. QIAprep Spin Miniprep Kit (Cat. No. 27104) was used for purification of the plasmid cDNA, followed by determination of cDNA concentration using spectrophotometer NanoDrop 2000 (ThermoScientificTM , Massachusetts, USA). .. Linearisation of plasmid DNA was performed and in vitro transcription was carried out with T7 Transcription Reaction Kit (RiboMaxTM Large Scale RNA production system), followed by DNase I treatment.

    Sequencing:

    Article Title: Discovering Protein-Protein Interactions Using Nucleic Acid Programmable Protein Arrays
    Article Snippet: Miniprep the query gene using the QIAprep Spin Miniprep kit and quantify DNA concentration with the NanoDrop Spectrophotometer. .. While both DNA miniprep and maxiprep are able to produce DNA of good quality as well as adequate concentration for sequencing or cloning purpose, the former is more time-efficient and can be done for a relatively large amount of DNA samples each time.

    Article Title: Functional Analyses of Transcription Factor Binding Sites that Differ between Present-Day and Archaic Humans
    Article Snippet: We synthesized a single-stranded oligonucleotide of 20 random bases (AAC CAT AGG ATT CGC GTC AGT CTA GAT AGN NNN NNN NNN NNN NNN NNN NGG CCG GCC GTC TGG CGT CTT GGC TAA TC) flanked on the 5’-end by an Xba I site and the sequence “TAG” and an Fse I site on the 3’-end (Invitrogen) as well as primer sites used to make the oligo double stranded by two cycles of polymerase chain reaction (PCR) (Thermo Scientific Phusion polymerase, annealing temperature 60 °C, primers AAC CAT AGG ATT CGC GTC AG and GAT TAG CCA AGA CGC CAG AC). .. DNA was extracted from individual clones using the Qiaprep Spin Miniprep Kit and colony PCR was performed (Thermo Scientific Phusion polymerase, annealing temperature 60 °C, primers TTT TTG TGT GAA TCG ATA GTA CTA ACA TAC GCT and GAG CTT CCA TTA TAT ACC CTC TAG TGT CTA AGC for regulatory regions, primers AGA TTC TCA TTA AGG CCA AGA AGG G and TCA CTG CAT TCT AGT TGT GGT TTG TCC for tag region).

    Article Title: Sea Star Wasting Disease in Asterias forbesi along the Atlantic Coast of North America
    Article Snippet: Paragraph title: Amplification, cloning and sequencing of SSaDV DNA from A . forbesi ... Plasmid DNA was isolated using a QiaPrep Spin MiniPrep kit and DNA concentration (μg/μL) was quantified using the Thermo Scientific Nanodrop ND8000 version 2.2.1 system.

    Article Title: Leptin in Whales: Validation and Measurement of mRNA Expression by Absolute Quantitative Real-Time PCR
    Article Snippet: Colonies were PCR checked for correct insertion and plasmid preparations completed using QIAprep® Spin Miniprep Kit (Invitrogen™). .. Plasmid preparations were quantified via Nanodrop® spectrophotometer as previously described and sequenced using BigDye® Terminator v3.1 Cycle Sequencing kit and an ABI 3130xl genetic analyzer (Applied Biosystems, Foster City, CA) to confirm sequence identities prior to real-time PCR assays.

    Article Title: Development of an Unbiased Antigen-Mining Approach To Identify Novel Vaccine Antigens and Diagnostic Reagents for Bovine Tuberculosis
    Article Snippet: DH5α transformants containing the petDest42 expression clones were selected by growth on ampicillin-supplemented LB+G (LB+G/amp+ ) agar plates and cultured overnight at 37°C in LB+G/amp+ broth to increase the yield of the expression clone, which was subsequently isolated using the QIAprep Spin miniprep kit and used to transform BL21(DE3) cells (Invitrogen). .. Sequencing of the inserts within the expression clones confirmed that they contained the M. tuberculosis gene of interest.

    CRISPR:

    Article Title: Targeted Mutation of NGN3 Gene Disrupts Pancreatic Endocrine Cell Development in Pigs
    Article Snippet: Purified gDNA was PCR amplified using KOD 2× PCR Master Mix (TOYOBO) over 35 cycles across NGN3 /CRISPR targeting site (Forward: 5′-CACCAGACCGAGCAGTCTTT-3′ Reverse: 3′-TTGGTGAGTTTCGCATCGT-5′). .. Plasmids were isolated from five to fifteen colonies using QIAprep spin miniprep kit, and DNA concentration determined using a NanoDrop ND-1000 spectrophotometer, followed by EcoRI restriction digest to confirm amplicon insertion.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Functional Analyses of Transcription Factor Binding Sites that Differ between Present-Day and Archaic Humans
    Article Snippet: .. DNA was extracted from individual clones using the Qiaprep Spin Miniprep Kit and colony PCR was performed (Thermo Scientific Phusion polymerase, annealing temperature 60 °C, primers TTT TTG TGT GAA TCG ATA GTA CTA ACA TAC GCT and GAG CTT CCA TTA TAT ACC CTC TAG TGT CTA AGC for regulatory regions, primers AGA TTC TCA TTA AGG CCA AGA AGG G and TCA CTG CAT TCT AGT TGT GGT TTG TCC for tag region). .. Plasmids that matched expected insert sizes for both amplifications were used to construct Illumina sequencing libraries ( ) and fully sequenced on an Illumina MiSeq (paired end 2 × (150 bp + 7 bp)) for sequence verification and association of the tags with TFBSs.

    Plasmid Preparation:

    Article Title: Discovering Protein-Protein Interactions Using Nucleic Acid Programmable Protein Arrays
    Article Snippet: Paragraph title: Construct the query gene expression vector ... Miniprep the query gene using the QIAprep Spin Miniprep kit and quantify DNA concentration with the NanoDrop Spectrophotometer.

    Article Title: Functional Analyses of Transcription Factor Binding Sites that Differ between Present-Day and Archaic Humans
    Article Snippet: Paragraph title: Cloning of TFBSs into pGL4.23 Plasmid ... DNA was extracted from individual clones using the Qiaprep Spin Miniprep Kit and colony PCR was performed (Thermo Scientific Phusion polymerase, annealing temperature 60 °C, primers TTT TTG TGT GAA TCG ATA GTA CTA ACA TAC GCT and GAG CTT CCA TTA TAT ACC CTC TAG TGT CTA AGC for regulatory regions, primers AGA TTC TCA TTA AGG CCA AGA AGG G and TCA CTG CAT TCT AGT TGT GGT TTG TCC for tag region).

    Article Title: Sea Star Wasting Disease in Asterias forbesi along the Atlantic Coast of North America
    Article Snippet: .. Plasmid DNA was isolated using a QiaPrep Spin MiniPrep kit and DNA concentration (μg/μL) was quantified using the Thermo Scientific Nanodrop ND8000 version 2.2.1 system. .. Samples were prepared for sequencing following standard procedure for the RI Genomics and Sequencing Center at the University of Rhode Island [ http://web.uri.edu/gsc/ ].

    Article Title: Targeted Mutation of NGN3 Gene Disrupts Pancreatic Endocrine Cell Development in Pigs
    Article Snippet: Products were purified over QIAprep DNA miniprep columns, subject to A-tailing and ligated into pCR™2.1 vector, and transformed into DH5α electrocompetent cells (prepared in-house). .. Plasmids were isolated from five to fifteen colonies using QIAprep spin miniprep kit, and DNA concentration determined using a NanoDrop ND-1000 spectrophotometer, followed by EcoRI restriction digest to confirm amplicon insertion.

    Article Title: Isoferulic acid prevents methylglyoxal-induced protein glycation and DNA damage by free radical scavenging activity
    Article Snippet: .. Plasmid extraction The pUC19 plasmid was purified from competent Escherichia coli by using QIAprep Spin Miniprep kit according to the manufacturer’s protocol and measured DNA concentration using the NanoDrop-1000 spectrophotometer (Thermo Scientific, MA, USA). ..

    Article Title: The Effects of 4-Hydroxybenzoic Acid Identified from Bamboo (Dendrocalamus asper) Shoots on Kv1.4 Channel
    Article Snippet: .. QIAprep Spin Miniprep Kit (Cat. No. 27104) was used for purification of the plasmid cDNA, followed by determination of cDNA concentration using spectrophotometer NanoDrop 2000 (ThermoScientificTM , Massachusetts, USA). .. Linearisation of plasmid DNA was performed and in vitro transcription was carried out with T7 Transcription Reaction Kit (RiboMaxTM Large Scale RNA production system), followed by DNase I treatment.

    Article Title: Leptin in Whales: Validation and Measurement of mRNA Expression by Absolute Quantitative Real-Time PCR
    Article Snippet: .. Colonies were PCR checked for correct insertion and plasmid preparations completed using QIAprep® Spin Miniprep Kit (Invitrogen™). .. Plasmid preparations were quantified via Nanodrop® spectrophotometer as previously described and sequenced using BigDye® Terminator v3.1 Cycle Sequencing kit and an ABI 3130xl genetic analyzer (Applied Biosystems, Foster City, CA) to confirm sequence identities prior to real-time PCR assays.

    Article Title: Development of an Unbiased Antigen-Mining Approach To Identify Novel Vaccine Antigens and Diagnostic Reagents for Bovine Tuberculosis
    Article Snippet: The M. tuberculosis gene of interest was transferred from the entry clone plasmid into the His-tag-containing petDest42 expression vector (Invitrogen, United Kingdom) using LR Clonase II (Invitrogen) and then was used to transform Library Efficiency DH5α cells (Invitrogen). .. DH5α transformants containing the petDest42 expression clones were selected by growth on ampicillin-supplemented LB+G (LB+G/amp+ ) agar plates and cultured overnight at 37°C in LB+G/amp+ broth to increase the yield of the expression clone, which was subsequently isolated using the QIAprep Spin miniprep kit and used to transform BL21(DE3) cells (Invitrogen).

    Software:

    Article Title: Targeted Mutation of NGN3 Gene Disrupts Pancreatic Endocrine Cell Development in Pigs
    Article Snippet: Plasmids were isolated from five to fifteen colonies using QIAprep spin miniprep kit, and DNA concentration determined using a NanoDrop ND-1000 spectrophotometer, followed by EcoRI restriction digest to confirm amplicon insertion. .. All sequences were aligned to wild type NGN3 using ClustalW available though BioEdit software.

    Electrophoresis:

    Article Title: Targeted Mutation of NGN3 Gene Disrupts Pancreatic Endocrine Cell Development in Pigs
    Article Snippet: Resulting products were separated using standard agarose gel electrophoresis and capillary electrophoresis using a Fragment Analyzer™(Advanced Analytical). .. Plasmids were isolated from five to fifteen colonies using QIAprep spin miniprep kit, and DNA concentration determined using a NanoDrop ND-1000 spectrophotometer, followed by EcoRI restriction digest to confirm amplicon insertion.

    Agarose Gel Electrophoresis:

    Article Title: Sea Star Wasting Disease in Asterias forbesi along the Atlantic Coast of North America
    Article Snippet: Samples were run through a Mastercycler® Nexus X2 (Eppendorf) with the following cycling conditions: 94°C for 10 min, then 35 cycles of 94°C for 30 s, 50°C for 30 s, 72°C for 90 s, with a final extension at 72°C for 5 min. Amplicons were run on a 1% agarose gel in TAE buffer (40 mM Tris, pH = 7.6, 20 mM acetic acid, 1 mM EDTA) for 30 min at 100 V, stained, destained and bands were visualized using the BioRad Quantity 1 System. .. Plasmid DNA was isolated using a QiaPrep Spin MiniPrep kit and DNA concentration (μg/μL) was quantified using the Thermo Scientific Nanodrop ND8000 version 2.2.1 system.

    Article Title: Targeted Mutation of NGN3 Gene Disrupts Pancreatic Endocrine Cell Development in Pigs
    Article Snippet: Resulting products were separated using standard agarose gel electrophoresis and capillary electrophoresis using a Fragment Analyzer™(Advanced Analytical). .. Plasmids were isolated from five to fifteen colonies using QIAprep spin miniprep kit, and DNA concentration determined using a NanoDrop ND-1000 spectrophotometer, followed by EcoRI restriction digest to confirm amplicon insertion.

    In Vitro:

    Article Title: The Effects of 4-Hydroxybenzoic Acid Identified from Bamboo (Dendrocalamus asper) Shoots on Kv1.4 Channel
    Article Snippet: Paragraph title: cDNA Cloning and in vitro Transcription ... QIAprep Spin Miniprep Kit (Cat. No. 27104) was used for purification of the plasmid cDNA, followed by determination of cDNA concentration using spectrophotometer NanoDrop 2000 (ThermoScientificTM , Massachusetts, USA).

    Ethanol Precipitation:

    Article Title: Targeted Mutation of NGN3 Gene Disrupts Pancreatic Endocrine Cell Development in Pigs
    Article Snippet: Following overnight digest, gDNA was extracted using phenol-chloroform, and recovered by resuspension in 100 µl of 10 mM Tris- HCl, pH 7.4 buffer following ethanol precipitation. .. Plasmids were isolated from five to fifteen colonies using QIAprep spin miniprep kit, and DNA concentration determined using a NanoDrop ND-1000 spectrophotometer, followed by EcoRI restriction digest to confirm amplicon insertion.

    Spectrophotometry:

    Article Title: Discovering Protein-Protein Interactions Using Nucleic Acid Programmable Protein Arrays
    Article Snippet: .. Miniprep the query gene using the QIAprep Spin Miniprep kit and quantify DNA concentration with the NanoDrop Spectrophotometer. .. While both DNA miniprep and maxiprep are able to produce DNA of good quality as well as adequate concentration for sequencing or cloning purpose, the former is more time-efficient and can be done for a relatively large amount of DNA samples each time.

    Article Title: Targeted Mutation of NGN3 Gene Disrupts Pancreatic Endocrine Cell Development in Pigs
    Article Snippet: .. Plasmids were isolated from five to fifteen colonies using QIAprep spin miniprep kit, and DNA concentration determined using a NanoDrop ND-1000 spectrophotometer, followed by EcoRI restriction digest to confirm amplicon insertion. .. An average of 10 colonies were used to confirm genotypes from both the ear and tail PCR amplicons.

    Article Title: Isoferulic acid prevents methylglyoxal-induced protein glycation and DNA damage by free radical scavenging activity
    Article Snippet: .. Plasmid extraction The pUC19 plasmid was purified from competent Escherichia coli by using QIAprep Spin Miniprep kit according to the manufacturer’s protocol and measured DNA concentration using the NanoDrop-1000 spectrophotometer (Thermo Scientific, MA, USA). ..

    Article Title: The Effects of 4-Hydroxybenzoic Acid Identified from Bamboo (Dendrocalamus asper) Shoots on Kv1.4 Channel
    Article Snippet: .. QIAprep Spin Miniprep Kit (Cat. No. 27104) was used for purification of the plasmid cDNA, followed by determination of cDNA concentration using spectrophotometer NanoDrop 2000 (ThermoScientificTM , Massachusetts, USA). .. Linearisation of plasmid DNA was performed and in vitro transcription was carried out with T7 Transcription Reaction Kit (RiboMaxTM Large Scale RNA production system), followed by DNase I treatment.

    Article Title: Leptin in Whales: Validation and Measurement of mRNA Expression by Absolute Quantitative Real-Time PCR
    Article Snippet: Colonies were PCR checked for correct insertion and plasmid preparations completed using QIAprep® Spin Miniprep Kit (Invitrogen™). .. Plasmid preparations were quantified via Nanodrop® spectrophotometer as previously described and sequenced using BigDye® Terminator v3.1 Cycle Sequencing kit and an ABI 3130xl genetic analyzer (Applied Biosystems, Foster City, CA) to confirm sequence identities prior to real-time PCR assays.

    Concentration Assay:

    Article Title: Discovering Protein-Protein Interactions Using Nucleic Acid Programmable Protein Arrays
    Article Snippet: .. Miniprep the query gene using the QIAprep Spin Miniprep kit and quantify DNA concentration with the NanoDrop Spectrophotometer. .. While both DNA miniprep and maxiprep are able to produce DNA of good quality as well as adequate concentration for sequencing or cloning purpose, the former is more time-efficient and can be done for a relatively large amount of DNA samples each time.

    Article Title: Sea Star Wasting Disease in Asterias forbesi along the Atlantic Coast of North America
    Article Snippet: .. Plasmid DNA was isolated using a QiaPrep Spin MiniPrep kit and DNA concentration (μg/μL) was quantified using the Thermo Scientific Nanodrop ND8000 version 2.2.1 system. .. Samples were prepared for sequencing following standard procedure for the RI Genomics and Sequencing Center at the University of Rhode Island [ http://web.uri.edu/gsc/ ].

    Article Title: Targeted Mutation of NGN3 Gene Disrupts Pancreatic Endocrine Cell Development in Pigs
    Article Snippet: .. Plasmids were isolated from five to fifteen colonies using QIAprep spin miniprep kit, and DNA concentration determined using a NanoDrop ND-1000 spectrophotometer, followed by EcoRI restriction digest to confirm amplicon insertion. .. An average of 10 colonies were used to confirm genotypes from both the ear and tail PCR amplicons.

    Article Title: Isoferulic acid prevents methylglyoxal-induced protein glycation and DNA damage by free radical scavenging activity
    Article Snippet: .. Plasmid extraction The pUC19 plasmid was purified from competent Escherichia coli by using QIAprep Spin Miniprep kit according to the manufacturer’s protocol and measured DNA concentration using the NanoDrop-1000 spectrophotometer (Thermo Scientific, MA, USA). ..

    Article Title: The Effects of 4-Hydroxybenzoic Acid Identified from Bamboo (Dendrocalamus asper) Shoots on Kv1.4 Channel
    Article Snippet: .. QIAprep Spin Miniprep Kit (Cat. No. 27104) was used for purification of the plasmid cDNA, followed by determination of cDNA concentration using spectrophotometer NanoDrop 2000 (ThermoScientificTM , Massachusetts, USA). .. Linearisation of plasmid DNA was performed and in vitro transcription was carried out with T7 Transcription Reaction Kit (RiboMaxTM Large Scale RNA production system), followed by DNase I treatment.

    Lysis:

    Article Title: Targeted Mutation of NGN3 Gene Disrupts Pancreatic Endocrine Cell Development in Pigs
    Article Snippet: Tissue biopsies (ear notch and tail dock) from fetuses and offspring were digested in a tissue lysis buffer (50 mM Tris pH 8.0, 0.1 M NaCl, 20 mM EDTA, 1% SDS, 50 µg/ml RNase A, 100 µg/ml proteinase K) overnight at 65 °C. .. Plasmids were isolated from five to fifteen colonies using QIAprep spin miniprep kit, and DNA concentration determined using a NanoDrop ND-1000 spectrophotometer, followed by EcoRI restriction digest to confirm amplicon insertion.

    Article Title: Development of an Unbiased Antigen-Mining Approach To Identify Novel Vaccine Antigens and Diagnostic Reagents for Bovine Tuberculosis
    Article Snippet: DH5α transformants containing the petDest42 expression clones were selected by growth on ampicillin-supplemented LB+G (LB+G/amp+ ) agar plates and cultured overnight at 37°C in LB+G/amp+ broth to increase the yield of the expression clone, which was subsequently isolated using the QIAprep Spin miniprep kit and used to transform BL21(DE3) cells (Invitrogen). .. Induced BL21(DE3) cells were pelleted by centrifugation, resuspended in binding buffer (8 M urea, 20 mM Tris-HCl, 500 mM NaCl, 50 mM imidazole, 1 mM β-mercaptoethanol [pH 7.4]), and mechanically disrupted using a FastPrep homogenizer (Thermo, United Kingdom) following the addition of lysis matrix.

    CTG Assay:

    Article Title: Functional Analyses of Transcription Factor Binding Sites that Differ between Present-Day and Archaic Humans
    Article Snippet: .. DNA was extracted from individual clones using the Qiaprep Spin Miniprep Kit and colony PCR was performed (Thermo Scientific Phusion polymerase, annealing temperature 60 °C, primers TTT TTG TGT GAA TCG ATA GTA CTA ACA TAC GCT and GAG CTT CCA TTA TAT ACC CTC TAG TGT CTA AGC for regulatory regions, primers AGA TTC TCA TTA AGG CCA AGA AGG G and TCA CTG CAT TCT AGT TGT GGT TTG TCC for tag region). .. Plasmids that matched expected insert sizes for both amplifications were used to construct Illumina sequencing libraries ( ) and fully sequenced on an Illumina MiSeq (paired end 2 × (150 bp + 7 bp)) for sequence verification and association of the tags with TFBSs.

    Gel Extraction:

    Article Title: Distinctive G Protein-Dependent Signaling by Protease-Activated Receptor 2 (PAR2) in Smooth Muscle: Feedback Inhibition of RhoA by cAMP-Independent PKA
    Article Snippet: .. Materials and Methods PAR2-activating peptide (PAR2-AP, SLIGRL) was obtained from Bachem, Torrance, CA; [125 I]cAMP, [γ-32 P]ATP, [32 P]Pi, [35 S]GTPγS, and [3 H]myo-inositol were obtained from Perkin Elmer Life Sciences, Boston, MA; Collagenase CLS type II and soybean trypsin inhibitor were obtained from Worthington, Freehold, NJ; Western blotting, Dowex AG-1×8 resin (100–200 mesh in formate form), chromatography material and protein assay kit from Bio-Rad Laboratories, Hercules, CA; antibodies to Gaq , Gai1 , Gai2 , Gai3 , Ga12 , Ga13 , Gas , phospho-specific MYPT1 (Thr696 ), MLC20 (Ser19 ) and CPI-17 (Thr38 ), Rho kinase, PKA catalytic subunit, IKK2(ser177/181 ), IkBa, and p65 subunit were from Santa Cruz Biotechnology, Santa Cruz, CA; myelin basic protein (MBP) was from Upstate Biotechnology; ML-9 was from Biomol Research Laboratories, Plymouth Meeting, PA; bisindolylmaleimide, Y27632, pertussis toxin, cAMP; Clostridium botulinum C3 exoenzyme, IKK-2 Inhibitor IV (IKK IV), MG-132, myristoylated PKI and U73122 were from Calbiochem, La Jolla, CA; RNAqueous™ kit was from Ambion, Austin, TX; Effectene Transfection Reagent, QIAEX®II Gel extraction Kit and QIAprep®Spin Miniprep Kit were from Germantown, MD; PCR reagents were from Applied Biosystems, Roche; SuperScript™ II Reverse Transcriptese and TOPO TA Cloning® Kit Dual Promoter were from Invitrogen, Grand Island, NY; EcoR I was from New England Bio Labs, Ipswich, MA; All other chemicals were from Sigma, St. Louis, MO. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Thermo Fisher qiaprep spin miniprep kit
    Qiaprep Spin Miniprep Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaprep spin miniprep kit/product/Thermo Fisher
    Average 96 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    qiaprep spin miniprep kit - by Bioz Stars, 2020-03
    96/100 stars
      Buy from Supplier

    Image Search Results