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TaKaRa qiaprep spin miniprep kit
Qiaprep Spin Miniprep Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/qiaprep spin miniprep kit/product/TaKaRa
Average 94 stars, based on 2 article reviews
Price from $9.99 to $1999.99
qiaprep spin miniprep kit - by Bioz Stars, 2020-04
94/100 stars

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Selection:

Article Title: Molecular Characterization and Biodiversity of a Putative Chlorotoxin from the Iranian Yellow Scorpion Odontobuthus doriae
Article Snippet: To select the positive colonies, random screening through the blue/white colony selection and colony PCR using flanking PCR primers were performed. .. The plasmid DNA of the selected colonies was extracted by QIAprep Spin Miniprep Kit (TAKARA Co., UK), and the cDNA inserts were sequenced (Macrogen® Co., Korea).

Synthesized:

Article Title: The HO-1-expressing bone mesenchymal stem cells protects intestine from ischemia and reperfusion injury
Article Snippet: Primers were designed with reference to the HO-1 sequence of GenBank, 5′-TTATGAATTCATGGAGCGTCCACAGCCC-3′ (EcoRI site); and 5′-CGCGGGATCCTTACATGGCTAAATTCCCACTGC-3′ (BamHI site) and synthesized by Gene Pharma company (Shanghai, China). .. A small quantity of plasmids was extracted by QIAprep Spin Miniprep Kit and sequenced by ABI377 DNA sequencer (Takara, Shanghai, China).

Ligation:

Article Title: Molecular Characterization and Biodiversity of a Putative Chlorotoxin from the Iranian Yellow Scorpion Odontobuthus doriae
Article Snippet: Ligation of cDNA into pSMART21F vector and transformation of vectors to chemically competent bacterial cells were done according to the suggested protocol by manufacturer. .. The plasmid DNA of the selected colonies was extracted by QIAprep Spin Miniprep Kit (TAKARA Co., UK), and the cDNA inserts were sequenced (Macrogen® Co., Korea).

Cell Culture:

Article Title: The HO-1-expressing bone mesenchymal stem cells protects intestine from ischemia and reperfusion injury
Article Snippet: A small quantity of plasmids was extracted by QIAprep Spin Miniprep Kit and sequenced by ABI377 DNA sequencer (Takara, Shanghai, China). .. BMSC were cultured in a 6-well plate (Corning, Inc., NY).

Polymerase Chain Reaction:

Article Title: Molecular Characterization and Biodiversity of a Putative Chlorotoxin from the Iranian Yellow Scorpion Odontobuthus doriae
Article Snippet: The selected PCR fragments corresponded to the expected length of toxin and venom components transcripts (around 500–1000 bp). .. The plasmid DNA of the selected colonies was extracted by QIAprep Spin Miniprep Kit (TAKARA Co., UK), and the cDNA inserts were sequenced (Macrogen® Co., Korea).

Article Title: The HO-1-expressing bone mesenchymal stem cells protects intestine from ischemia and reperfusion injury
Article Snippet: Then the PCR products were ligased with pEGFP-N1 and transformed into E. coli DH5α. .. A small quantity of plasmids was extracted by QIAprep Spin Miniprep Kit and sequenced by ABI377 DNA sequencer (Takara, Shanghai, China).

cDNA Library Assay:

Article Title: Molecular Characterization and Biodiversity of a Putative Chlorotoxin from the Iranian Yellow Scorpion Odontobuthus doriae
Article Snippet: Paragraph title: The cDNA library construction ... The plasmid DNA of the selected colonies was extracted by QIAprep Spin Miniprep Kit (TAKARA Co., UK), and the cDNA inserts were sequenced (Macrogen® Co., Korea).

Sequencing:

Article Title: The HO-1-expressing bone mesenchymal stem cells protects intestine from ischemia and reperfusion injury
Article Snippet: Primers were designed with reference to the HO-1 sequence of GenBank, 5′-TTATGAATTCATGGAGCGTCCACAGCCC-3′ (EcoRI site); and 5′-CGCGGGATCCTTACATGGCTAAATTCCCACTGC-3′ (BamHI site) and synthesized by Gene Pharma company (Shanghai, China). .. A small quantity of plasmids was extracted by QIAprep Spin Miniprep Kit and sequenced by ABI377 DNA sequencer (Takara, Shanghai, China).

Gel Extraction:

Article Title: The HO-1-expressing bone mesenchymal stem cells protects intestine from ischemia and reperfusion injury
Article Snippet: In this case, 2 μL cDNA was added into 50 μL total volume, and the reaction conditions are 94 °C 4 min, then 35 cycles of 94 °C 30 s, 55 °C 30 s and 72 °C 2 min, at last 72 °C 10 min. PCR product was digested by EcoRI+ BamHI double enzymes recovered from the gel by QIAquick Gel Extraction Kit. .. A small quantity of plasmids was extracted by QIAprep Spin Miniprep Kit and sequenced by ABI377 DNA sequencer (Takara, Shanghai, China).

Transformation Assay:

Article Title: Molecular Characterization and Biodiversity of a Putative Chlorotoxin from the Iranian Yellow Scorpion Odontobuthus doriae
Article Snippet: Transformed cells grown on Luria Bertani agar plate contained 100 µg/ml ampicillin, 1 mM isopropyl-beta-D-thiogalacto-pyranoside, and 75 µg/ml 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside. .. The plasmid DNA of the selected colonies was extracted by QIAprep Spin Miniprep Kit (TAKARA Co., UK), and the cDNA inserts were sequenced (Macrogen® Co., Korea).

Article Title: The HO-1-expressing bone mesenchymal stem cells protects intestine from ischemia and reperfusion injury
Article Snippet: Then the PCR products were ligased with pEGFP-N1 and transformed into E. coli DH5α. .. A small quantity of plasmids was extracted by QIAprep Spin Miniprep Kit and sequenced by ABI377 DNA sequencer (Takara, Shanghai, China).

Plasmid Preparation:

Article Title: Molecular Characterization and Biodiversity of a Putative Chlorotoxin from the Iranian Yellow Scorpion Odontobuthus doriae
Article Snippet: .. The plasmid DNA of the selected colonies was extracted by QIAprep Spin Miniprep Kit (TAKARA Co., UK), and the cDNA inserts were sequenced (Macrogen® Co., Korea). .. Bioinformatics analysis cDNA sequence of chloride toxin was checked by VecScreen tools ( http://www.ncbi.nlm.nih.gov/tools/vecscreen/ ) to trim from vector and primers sequence contaminations.

Article Title: The HO-1-expressing bone mesenchymal stem cells protects intestine from ischemia and reperfusion injury
Article Snippet: Lentivirus carrying EGFP (vector) was supplied by Inovogen Tech. .. A small quantity of plasmids was extracted by QIAprep Spin Miniprep Kit and sequenced by ABI377 DNA sequencer (Takara, Shanghai, China).

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    TaKaRa qiaprep spin miniprep kit
    Qiaprep Spin Miniprep Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaprep spin miniprep kit/product/TaKaRa
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    qiaprep spin miniprep kit - by Bioz Stars, 2020-04
    94/100 stars
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