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Retrogen qiaprep spin miniprep kit
Qiaprep Spin Miniprep Kit, supplied by Retrogen, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/qiaprep spin miniprep kit/product/Retrogen
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
qiaprep spin miniprep kit - by Bioz Stars, 2020-04
93/100 stars

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Countercurrent Chromatography:

Article Title: A Simplified Protocol for High-Yield Expression and Purification of Bacterial Topoisomerase I
Article Snippet: Upon successful transformation, the plasmid was isolated with the QIAprep Spin Miniprep Kit via protocol and the sequence was confirmed via Retrogen, Inc. (San Diego, CA). .. For the pET 21d vector, the SmTopA gene was amplified via PCR around NcoI and BamHI restriction sites using the following primers: NcoI_Site_For: TAT ACG ATG GGC AGC AGC CAT CAT C NdeI_Site_For: GGC AGC CCC ATG GCA AGT AAA AC BamHI_Site_Rev: TTC GGA TCC TTT TTA ACA GCT TTT TCC TTA TAG TCA CAC PCR was carried out via Platinum® PCR SuperMix High Fidelity protocol and denatured at 94°C for 30 seconds, annealed at 58°C for 45 seconds, and extended at 68°C for two minutes and thirty seconds for 35 cycles and then held at 4 °C.

Clone Assay:

Article Title: A Simplified Protocol for High-Yield Expression and Purification of Bacterial Topoisomerase I
Article Snippet: Paragraph title: Cloning and Plasmid Construction ... Upon successful transformation, the plasmid was isolated with the QIAprep Spin Miniprep Kit via protocol and the sequence was confirmed via Retrogen, Inc. (San Diego, CA).

Positron Emission Tomography:

Article Title: A Simplified Protocol for High-Yield Expression and Purification of Bacterial Topoisomerase I
Article Snippet: The pET-15b vector was then digested with NdeI and BamHI restriction enzymes per New England Biolabs (NEB) protocol, purified, then mixed and ligated with the SmTopA_pET PCR product via NEB Quick Ligation™ Kit protocol. .. Upon successful transformation, the plasmid was isolated with the QIAprep Spin Miniprep Kit via protocol and the sequence was confirmed via Retrogen, Inc. (San Diego, CA).

Agarose Gel Electrophoresis:

Article Title: A Simplified Protocol for High-Yield Expression and Purification of Bacterial Topoisomerase I
Article Snippet: Success was analyzed via 1% agarose gel electrophoresis based upon the confirmed presence of the ∼2Kbp SmTopA_pET PCR product. .. Upon successful transformation, the plasmid was isolated with the QIAprep Spin Miniprep Kit via protocol and the sequence was confirmed via Retrogen, Inc. (San Diego, CA).

Ligation:

Article Title: A Simplified Protocol for High-Yield Expression and Purification of Bacterial Topoisomerase I
Article Snippet: The ligation product (SmTopA15b) was then transformed into XL1-Blue Supercompetent cells per protocol. .. Upon successful transformation, the plasmid was isolated with the QIAprep Spin Miniprep Kit via protocol and the sequence was confirmed via Retrogen, Inc. (San Diego, CA).

Isolation:

Article Title: A Simplified Protocol for High-Yield Expression and Purification of Bacterial Topoisomerase I
Article Snippet: .. Upon successful transformation, the plasmid was isolated with the QIAprep Spin Miniprep Kit via protocol and the sequence was confirmed via Retrogen, Inc. (San Diego, CA). .. The plasmid was also transformed into BL21(DE3)-Gold Competent cells via protocol.

Purification:

Article Title: A Simplified Protocol for High-Yield Expression and Purification of Bacterial Topoisomerase I
Article Snippet: The pET-15b vector was then digested with NdeI and BamHI restriction enzymes per New England Biolabs (NEB) protocol, purified, then mixed and ligated with the SmTopA_pET PCR product via NEB Quick Ligation™ Kit protocol. .. Upon successful transformation, the plasmid was isolated with the QIAprep Spin Miniprep Kit via protocol and the sequence was confirmed via Retrogen, Inc. (San Diego, CA).

Sequencing:

Article Title: A Simplified Protocol for High-Yield Expression and Purification of Bacterial Topoisomerase I
Article Snippet: .. Upon successful transformation, the plasmid was isolated with the QIAprep Spin Miniprep Kit via protocol and the sequence was confirmed via Retrogen, Inc. (San Diego, CA). .. The plasmid was also transformed into BL21(DE3)-Gold Competent cells via protocol.

Amplification:

Article Title: A Simplified Protocol for High-Yield Expression and Purification of Bacterial Topoisomerase I
Article Snippet: Upon successful transformation, the plasmid was isolated with the QIAprep Spin Miniprep Kit via protocol and the sequence was confirmed via Retrogen, Inc. (San Diego, CA). .. For the pET 21d vector, the SmTopA gene was amplified via PCR around NcoI and BamHI restriction sites using the following primers: NcoI_Site_For: TAT ACG ATG GGC AGC AGC CAT CAT C NdeI_Site_For: GGC AGC CCC ATG GCA AGT AAA AC BamHI_Site_Rev: TTC GGA TCC TTT TTA ACA GCT TTT TCC TTA TAG TCA CAC PCR was carried out via Platinum® PCR SuperMix High Fidelity protocol and denatured at 94°C for 30 seconds, annealed at 58°C for 45 seconds, and extended at 68°C for two minutes and thirty seconds for 35 cycles and then held at 4 °C.

Polymerase Chain Reaction:

Article Title: A Simplified Protocol for High-Yield Expression and Purification of Bacterial Topoisomerase I
Article Snippet: The pET-15b vector was then digested with NdeI and BamHI restriction enzymes per New England Biolabs (NEB) protocol, purified, then mixed and ligated with the SmTopA_pET PCR product via NEB Quick Ligation™ Kit protocol. .. Upon successful transformation, the plasmid was isolated with the QIAprep Spin Miniprep Kit via protocol and the sequence was confirmed via Retrogen, Inc. (San Diego, CA).

Transformation Assay:

Article Title: A Simplified Protocol for High-Yield Expression and Purification of Bacterial Topoisomerase I
Article Snippet: .. Upon successful transformation, the plasmid was isolated with the QIAprep Spin Miniprep Kit via protocol and the sequence was confirmed via Retrogen, Inc. (San Diego, CA). .. The plasmid was also transformed into BL21(DE3)-Gold Competent cells via protocol.

Chloramphenicol Acetyltransferase Assay:

Article Title: A Simplified Protocol for High-Yield Expression and Purification of Bacterial Topoisomerase I
Article Snippet: Initially, the topA gene was cloned from Streptococcus mutans UA159 genomic DNA for insertion into the pET-15b vector based for the NdeI and BamHI restriction sites using the following primers: TopA_Forward: 5′-TGT AGA CAT ATG ACA AGT AAA ACA ACG ACA ACA-3′ TopA_Reverse: 5′-TCA TCA GGA TCC TTA TTT AAC AGC TTT TTC CTT-3′ PCR was carried out via Platinum® PCR SuperMix High Fidelity protocol and denatured at 94 °C for 30 seconds, annealed at 58 °C for 40 seconds, and extended at 68 °C for two minutes for 30 cycles and then held at 4 °C. .. Upon successful transformation, the plasmid was isolated with the QIAprep Spin Miniprep Kit via protocol and the sequence was confirmed via Retrogen, Inc. (San Diego, CA).

Plasmid Preparation:

Article Title: A Simplified Protocol for High-Yield Expression and Purification of Bacterial Topoisomerase I
Article Snippet: .. Upon successful transformation, the plasmid was isolated with the QIAprep Spin Miniprep Kit via protocol and the sequence was confirmed via Retrogen, Inc. (San Diego, CA). .. The plasmid was also transformed into BL21(DE3)-Gold Competent cells via protocol.

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    Retrogen qiaprep spin miniprep kit
    Qiaprep Spin Miniprep Kit, supplied by Retrogen, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaprep spin miniprep kit/product/Retrogen
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qiaprep spin miniprep kit - by Bioz Stars, 2020-04
    93/100 stars
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