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Promega qiaprep spin miniprep kit
Qiaprep Spin Miniprep Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/qiaprep spin miniprep kit/product/Promega
Average 90 stars, based on 2 article reviews
Price from $9.99 to $1999.99
qiaprep spin miniprep kit - by Bioz Stars, 2020-03
90/100 stars

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Clone Assay:

Article Title: Serologic Prevalence of MPV1 in Mouse Strains in a Commercial Laboratory Mouse Colony Determined by Using VP1 Antigen
Article Snippet: The plasmid DNA was purified with a QIAprep Spin Miniprep Kit (Promega) by using the protocol described by the manufacturer. .. The endonuclease Pvu II, with the recognition sequence CAGCTG, was chosen because there was a single Pvu II restriction site within the multiple cloning region of the PinPoint Xa1 Vector and a single Pvu II restriction site within the putative allotropic determinant.

Article Title: Epidemiology and Clinical Presentations of the Four Human Coronaviruses 229E, HKU1, NL63, and OC43 Detected over 3 Years Using a Novel Multiplex Real-Time PCR Method ▿
Article Snippet: PCR products were cloned into PCR2.1 T/A cloning vector (Invitrogen, Paisley, United Kingdom) according to the manufacturer's instructions for transformation of electrocompetent Escherichia coli . .. Products were purified using a Qiagen (Crawley, United Kingdom) Qiaprep spin miniprep kit and were transcribed in vitro using a Promega (Southampton, United Kingdom) T7 RiboMAX large-scale RNA production system.

Multiplex Assay:

Article Title: Epidemiology and Clinical Presentations of the Four Human Coronaviruses 229E, HKU1, NL63, and OC43 Detected over 3 Years Using a Novel Multiplex Real-Time PCR Method ▿
Article Snippet: Paragraph title: Sensitivity of real-time multiplex PCR for coronavirus detection. ... Products were purified using a Qiagen (Crawley, United Kingdom) Qiaprep spin miniprep kit and were transcribed in vitro using a Promega (Southampton, United Kingdom) T7 RiboMAX large-scale RNA production system.

Amplification:

Article Title: Serologic Prevalence of MPV1 in Mouse Strains in a Commercial Laboratory Mouse Colony Determined by Using VP1 Antigen
Article Snippet: The amplified PCR products was excised and purified by using the Wizard PCR Purification System (Promega) and ligated into the PinPoint Xa1 vector (Promega) as specified by the manufacturer. .. The plasmid DNA was purified with a QIAprep Spin Miniprep Kit (Promega) by using the protocol described by the manufacturer.

Article Title: Epidemiology and Clinical Presentations of the Four Human Coronaviruses 229E, HKU1, NL63, and OC43 Detected over 3 Years Using a Novel Multiplex Real-Time PCR Method ▿
Article Snippet: Products were purified using a Qiagen (Crawley, United Kingdom) Qiaprep spin miniprep kit and were transcribed in vitro using a Promega (Southampton, United Kingdom) T7 RiboMAX large-scale RNA production system. .. HCoV-HKU1-positive cDNA underwent PCR, using a sense primer incorporating a T7 promoter site for DNA amplification.

Agarose Gel Electrophoresis:

Article Title: Glycosylation of acyl carrier protein-bound polyketides during pactamycin biosynthesis
Article Snippet: DNA fragments were recovered from an agarose gel by using the QIAquick Gel Extraction Kit (QIAGEN). .. Preparation of plasmid DNA was done by using a QIAprep Spin Miniprep Kit (Promega).

In Vitro:

Article Title: Epidemiology and Clinical Presentations of the Four Human Coronaviruses 229E, HKU1, NL63, and OC43 Detected over 3 Years Using a Novel Multiplex Real-Time PCR Method ▿
Article Snippet: .. Products were purified using a Qiagen (Crawley, United Kingdom) Qiaprep spin miniprep kit and were transcribed in vitro using a Promega (Southampton, United Kingdom) T7 RiboMAX large-scale RNA production system. .. HCoV-HKU1-positive cDNA underwent PCR, using a sense primer incorporating a T7 promoter site for DNA amplification.

Synthesized:

Article Title: Glycosylation of acyl carrier protein-bound polyketides during pactamycin biosynthesis
Article Snippet: Preparation of plasmid DNA was done by using a QIAprep Spin Miniprep Kit (Promega). .. Oligodeoxyribonucleotides for PCR primers were synthesized by Sigma-Genosys.

Purification:

Article Title: Serologic Prevalence of MPV1 in Mouse Strains in a Commercial Laboratory Mouse Colony Determined by Using VP1 Antigen
Article Snippet: .. The plasmid DNA was purified with a QIAprep Spin Miniprep Kit (Promega) by using the protocol described by the manufacturer. .. The orientation of the insert was determined by restriction endonuclease mapping and was based on the known sequence of the PinPoint Xa1 Vector and VP1 sequence.

Article Title: Epidemiology and Clinical Presentations of the Four Human Coronaviruses 229E, HKU1, NL63, and OC43 Detected over 3 Years Using a Novel Multiplex Real-Time PCR Method ▿
Article Snippet: .. Products were purified using a Qiagen (Crawley, United Kingdom) Qiaprep spin miniprep kit and were transcribed in vitro using a Promega (Southampton, United Kingdom) T7 RiboMAX large-scale RNA production system. .. HCoV-HKU1-positive cDNA underwent PCR, using a sense primer incorporating a T7 promoter site for DNA amplification.

Real-time Polymerase Chain Reaction:

Article Title: Epidemiology and Clinical Presentations of the Four Human Coronaviruses 229E, HKU1, NL63, and OC43 Detected over 3 Years Using a Novel Multiplex Real-Time PCR Method ▿
Article Snippet: HCoV-229E, HCoV-NL63, and HCoV-OC43 cDNAs (processed using nucleic acid extraction and reverse transcription as described below) were subjected to single-round real-time PCR as described below. .. Products were purified using a Qiagen (Crawley, United Kingdom) Qiaprep spin miniprep kit and were transcribed in vitro using a Promega (Southampton, United Kingdom) T7 RiboMAX large-scale RNA production system.

Polymerase Chain Reaction:

Article Title: Glycosylation of acyl carrier protein-bound polyketides during pactamycin biosynthesis
Article Snippet: Preparation of plasmid DNA was done by using a QIAprep Spin Miniprep Kit (Promega). .. All other DNA manipulations were performed according to standard protocols., PCR was performed in 30 cycles by using a Mastercycler gradient thermocycler (Eppendorf) and Platinum Taq high fidelity DNA polymerase (Invitrogen).

Article Title: Serologic Prevalence of MPV1 in Mouse Strains in a Commercial Laboratory Mouse Colony Determined by Using VP1 Antigen
Article Snippet: Colonies were picked by using sterile toothpicks into individual 1.5-mL sterile microcentrifuge tubes containing 50 μL PCR-grade water. .. The plasmid DNA was purified with a QIAprep Spin Miniprep Kit (Promega) by using the protocol described by the manufacturer.

Article Title: Epidemiology and Clinical Presentations of the Four Human Coronaviruses 229E, HKU1, NL63, and OC43 Detected over 3 Years Using a Novel Multiplex Real-Time PCR Method ▿
Article Snippet: Paragraph title: Sensitivity of real-time multiplex PCR for coronavirus detection. ... Products were purified using a Qiagen (Crawley, United Kingdom) Qiaprep spin miniprep kit and were transcribed in vitro using a Promega (Southampton, United Kingdom) T7 RiboMAX large-scale RNA production system.

Incubation:

Article Title: Serologic Prevalence of MPV1 in Mouse Strains in a Commercial Laboratory Mouse Colony Determined by Using VP1 Antigen
Article Snippet: The plasmid DNA was purified with a QIAprep Spin Miniprep Kit (Promega) by using the protocol described by the manufacturer. .. Small-scale cultures of E. coli JM109 carrying the PinPoint recombinant plasmid were inoculated into 5 mL LB medium containing 100 μg/mL ampicillin and 2 μM biotin and incubated overnight at 37 °C with shaking.

DNA Purification:

Article Title: Human fluids alter DNA-acquisition in Acinetobacter baumannii
Article Snippet: .. Total genomic DNA from A. baumannii Ab144 and plasmid DNA from Escherichia coli TOP10 cells harboring pDsRedAK were obtained using Wizard® Genomic DNA Purification Kit and QIAprep Spin Miniprep Kit following manufacturer instructions (Promega, Madison, WI and Qiagen Germantown, MD, USA), respectively. .. Natural transformation assays were done as previously described [ ].

Sequencing:

Article Title: Serologic Prevalence of MPV1 in Mouse Strains in a Commercial Laboratory Mouse Colony Determined by Using VP1 Antigen
Article Snippet: The plasmid DNA was purified with a QIAprep Spin Miniprep Kit (Promega) by using the protocol described by the manufacturer. .. The orientation of the insert was determined by restriction endonuclease mapping and was based on the known sequence of the PinPoint Xa1 Vector and VP1 sequence.

Article Title: Epidemiology and Clinical Presentations of the Four Human Coronaviruses 229E, HKU1, NL63, and OC43 Detected over 3 Years Using a Novel Multiplex Real-Time PCR Method ▿
Article Snippet: Blue colonies were picked, and successful transformation was confirmed by sequence analysis. .. Products were purified using a Qiagen (Crawley, United Kingdom) Qiaprep spin miniprep kit and were transcribed in vitro using a Promega (Southampton, United Kingdom) T7 RiboMAX large-scale RNA production system.

Gel Extraction:

Article Title: Glycosylation of acyl carrier protein-bound polyketides during pactamycin biosynthesis
Article Snippet: DNA fragments were recovered from an agarose gel by using the QIAquick Gel Extraction Kit (QIAGEN). .. Preparation of plasmid DNA was done by using a QIAprep Spin Miniprep Kit (Promega).

Transformation Assay:

Article Title: Human fluids alter DNA-acquisition in Acinetobacter baumannii
Article Snippet: Acinetobacter baumannii clinical strains A118 and A42 were used for transformation assays. .. Total genomic DNA from A. baumannii Ab144 and plasmid DNA from Escherichia coli TOP10 cells harboring pDsRedAK were obtained using Wizard® Genomic DNA Purification Kit and QIAprep Spin Miniprep Kit following manufacturer instructions (Promega, Madison, WI and Qiagen Germantown, MD, USA), respectively.

Article Title: Epidemiology and Clinical Presentations of the Four Human Coronaviruses 229E, HKU1, NL63, and OC43 Detected over 3 Years Using a Novel Multiplex Real-Time PCR Method ▿
Article Snippet: Blue colonies were picked, and successful transformation was confirmed by sequence analysis. .. Products were purified using a Qiagen (Crawley, United Kingdom) Qiaprep spin miniprep kit and were transcribed in vitro using a Promega (Southampton, United Kingdom) T7 RiboMAX large-scale RNA production system.

Recombinant:

Article Title: Serologic Prevalence of MPV1 in Mouse Strains in a Commercial Laboratory Mouse Colony Determined by Using VP1 Antigen
Article Snippet: Paragraph title: Recombinant truncated MPV1f VP1 capsid protein. ... The plasmid DNA was purified with a QIAprep Spin Miniprep Kit (Promega) by using the protocol described by the manufacturer.

Plasmid Preparation:

Article Title: Glycosylation of acyl carrier protein-bound polyketides during pactamycin biosynthesis
Article Snippet: .. Preparation of plasmid DNA was done by using a QIAprep Spin Miniprep Kit (Promega). .. All other DNA manipulations were performed according to standard protocols., PCR was performed in 30 cycles by using a Mastercycler gradient thermocycler (Eppendorf) and Platinum Taq high fidelity DNA polymerase (Invitrogen).

Article Title: Serologic Prevalence of MPV1 in Mouse Strains in a Commercial Laboratory Mouse Colony Determined by Using VP1 Antigen
Article Snippet: .. The plasmid DNA was purified with a QIAprep Spin Miniprep Kit (Promega) by using the protocol described by the manufacturer. .. The orientation of the insert was determined by restriction endonuclease mapping and was based on the known sequence of the PinPoint Xa1 Vector and VP1 sequence.

Article Title: Human fluids alter DNA-acquisition in Acinetobacter baumannii
Article Snippet: .. Total genomic DNA from A. baumannii Ab144 and plasmid DNA from Escherichia coli TOP10 cells harboring pDsRedAK were obtained using Wizard® Genomic DNA Purification Kit and QIAprep Spin Miniprep Kit following manufacturer instructions (Promega, Madison, WI and Qiagen Germantown, MD, USA), respectively. .. Natural transformation assays were done as previously described [ ].

Article Title: Epidemiology and Clinical Presentations of the Four Human Coronaviruses 229E, HKU1, NL63, and OC43 Detected over 3 Years Using a Novel Multiplex Real-Time PCR Method ▿
Article Snippet: PCR products were cloned into PCR2.1 T/A cloning vector (Invitrogen, Paisley, United Kingdom) according to the manufacturer's instructions for transformation of electrocompetent Escherichia coli . .. Products were purified using a Qiagen (Crawley, United Kingdom) Qiaprep spin miniprep kit and were transcribed in vitro using a Promega (Southampton, United Kingdom) T7 RiboMAX large-scale RNA production system.

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    Promega qiaprep spin miniprep kit
    Qiaprep Spin Miniprep Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaprep spin miniprep kit/product/Promega
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    qiaprep spin miniprep kit - by Bioz Stars, 2020-03
    90/100 stars
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