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Genewiz qiaprep spin miniprep kit
Qiaprep Spin Miniprep Kit, supplied by Genewiz, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/qiaprep spin miniprep kit/product/Genewiz
Average 93 stars, based on 2 article reviews
Price from $9.99 to $1999.99
qiaprep spin miniprep kit - by Bioz Stars, 2020-04
93/100 stars

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Related Articles

Clone Assay:

Article Title: Four key steps control glycolytic flux in mammalian cells
Article Snippet: Paragraph title: Cloning of transient overexpression and lentivirus constructs ... Plasmid DNA was prepared by the QIAprep Spin Miniprep Kit and constructs were sequence verified by Sanger sequencing (Genewiz, South Plainfield, NJ, USA).

Article Title: Cortisol Synthesis in Epidermis Is Induced by IL-1 and Tissue Injury *
Article Snippet: .. We confirmed primers by cloning PCR products with TOPO-TA One Shot Chemical Transformation, isolated DNA using the QIAprep Spin Miniprep kit, and sequenced the plasmids through Genewiz. .. To establish the level of activity of the GC pathway in epidermis, we stained six different specimens of normal human skin with antiphospho-Ser211 GR antibody that recognizes ligand-induced phosphorylation at Ser211 ( ).

Amplification:

Article Title: Four key steps control glycolytic flux in mammalian cells
Article Snippet: Briefly, the vector was linearized by backbone PCR and the ORFs were amplified by PCR as described above. .. Plasmid DNA was prepared by the QIAprep Spin Miniprep Kit and constructs were sequence verified by Sanger sequencing (Genewiz, South Plainfield, NJ, USA).

Agarose Gel Electrophoresis:

Article Title: Four key steps control glycolytic flux in mammalian cells
Article Snippet: The linearized vector and PCR products were run on a 1% agarose gel (with ethidium bromide) and the cut bands were cleaned up with the Wizard® SV Gel and PCR Clean-Up System. .. Plasmid DNA was prepared by the QIAprep Spin Miniprep Kit and constructs were sequence verified by Sanger sequencing (Genewiz, South Plainfield, NJ, USA).

Isolation:

Article Title: Cortisol Synthesis in Epidermis Is Induced by IL-1 and Tissue Injury *
Article Snippet: .. We confirmed primers by cloning PCR products with TOPO-TA One Shot Chemical Transformation, isolated DNA using the QIAprep Spin Miniprep kit, and sequenced the plasmids through Genewiz. .. To establish the level of activity of the GC pathway in epidermis, we stained six different specimens of normal human skin with antiphospho-Ser211 GR antibody that recognizes ligand-induced phosphorylation at Ser211 ( ).

Construct:

Article Title: Four key steps control glycolytic flux in mammalian cells
Article Snippet: .. Plasmid DNA was prepared by the QIAprep Spin Miniprep Kit and constructs were sequence verified by Sanger sequencing (Genewiz, South Plainfield, NJ, USA). .. iBMK cells were transfected using Lipofectamine 2000 as described in the manufacturer’s protocol.

SYBR Green Assay:

Article Title: Cortisol Synthesis in Epidermis Is Induced by IL-1 and Tissue Injury *
Article Snippet: Real time PCR (RT-qPCR) was performed in triplicate using the iCycler iQ thermal cycler and detection system and Qscript 1 step RT along with SYBR Green (Bio-Rad). .. We confirmed primers by cloning PCR products with TOPO-TA One Shot Chemical Transformation, isolated DNA using the QIAprep Spin Miniprep kit, and sequenced the plasmids through Genewiz.

Sequencing:

Article Title: Four key steps control glycolytic flux in mammalian cells
Article Snippet: .. Plasmid DNA was prepared by the QIAprep Spin Miniprep Kit and constructs were sequence verified by Sanger sequencing (Genewiz, South Plainfield, NJ, USA). .. iBMK cells were transfected using Lipofectamine 2000 as described in the manufacturer’s protocol.

Article Title: Cortisol Synthesis in Epidermis Is Induced by IL-1 and Tissue Injury *
Article Snippet: The primer sequence for CYP11B1 was forward (5′-GCGGAGGTGTCGGTAGGC-3′) and reverse (5′-CTTCCACCTTCTGACACCGCT-3′). .. We confirmed primers by cloning PCR products with TOPO-TA One Shot Chemical Transformation, isolated DNA using the QIAprep Spin Miniprep kit, and sequenced the plasmids through Genewiz.

Quantitative RT-PCR:

Article Title: Cortisol Synthesis in Epidermis Is Induced by IL-1 and Tissue Injury *
Article Snippet: Real time PCR (RT-qPCR) was performed in triplicate using the iCycler iQ thermal cycler and detection system and Qscript 1 step RT along with SYBR Green (Bio-Rad). .. We confirmed primers by cloning PCR products with TOPO-TA One Shot Chemical Transformation, isolated DNA using the QIAprep Spin Miniprep kit, and sequenced the plasmids through Genewiz.

Expressing:

Article Title: Cortisol Synthesis in Epidermis Is Induced by IL-1 and Tissue Injury *
Article Snippet: Relative expression was normalized for levels of GAPDH forward (5′-ACATCATCCCTGCTTCTAC-3′) and reverse (5′-TGCTTCACCACCTTCTTG-3) and B2M forward (5′-CCTGCTCGGGCTGCTCTC-3′) and reverse (5′-GTGGCGTGAGTAAACCTGAACC-3′). .. We confirmed primers by cloning PCR products with TOPO-TA One Shot Chemical Transformation, isolated DNA using the QIAprep Spin Miniprep kit, and sequenced the plasmids through Genewiz.

Polymerase Chain Reaction:

Article Title: Four key steps control glycolytic flux in mammalian cells
Article Snippet: The linearized vector and PCR products were run on a 1% agarose gel (with ethidium bromide) and the cut bands were cleaned up with the Wizard® SV Gel and PCR Clean-Up System. .. Plasmid DNA was prepared by the QIAprep Spin Miniprep Kit and constructs were sequence verified by Sanger sequencing (Genewiz, South Plainfield, NJ, USA).

Article Title: Cortisol Synthesis in Epidermis Is Induced by IL-1 and Tissue Injury *
Article Snippet: .. We confirmed primers by cloning PCR products with TOPO-TA One Shot Chemical Transformation, isolated DNA using the QIAprep Spin Miniprep kit, and sequenced the plasmids through Genewiz. .. To establish the level of activity of the GC pathway in epidermis, we stained six different specimens of normal human skin with antiphospho-Ser211 GR antibody that recognizes ligand-induced phosphorylation at Ser211 ( ).

Real-time Polymerase Chain Reaction:

Article Title: Cortisol Synthesis in Epidermis Is Induced by IL-1 and Tissue Injury *
Article Snippet: Paragraph title: Real Time qPCR ... We confirmed primers by cloning PCR products with TOPO-TA One Shot Chemical Transformation, isolated DNA using the QIAprep Spin Miniprep kit, and sequenced the plasmids through Genewiz.

Over Expression:

Article Title: Four key steps control glycolytic flux in mammalian cells
Article Snippet: Paragraph title: Cloning of transient overexpression and lentivirus constructs ... Plasmid DNA was prepared by the QIAprep Spin Miniprep Kit and constructs were sequence verified by Sanger sequencing (Genewiz, South Plainfield, NJ, USA).

Transformation Assay:

Article Title: Four key steps control glycolytic flux in mammalian cells
Article Snippet: Assembled constructs were transformed into Clontech Stellar competent E. coli cells and grown on agar plates (50 μg/mL carbenicillin) at 37 °C overnight. .. Plasmid DNA was prepared by the QIAprep Spin Miniprep Kit and constructs were sequence verified by Sanger sequencing (Genewiz, South Plainfield, NJ, USA).

Article Title: Cortisol Synthesis in Epidermis Is Induced by IL-1 and Tissue Injury *
Article Snippet: .. We confirmed primers by cloning PCR products with TOPO-TA One Shot Chemical Transformation, isolated DNA using the QIAprep Spin Miniprep kit, and sequenced the plasmids through Genewiz. .. To establish the level of activity of the GC pathway in epidermis, we stained six different specimens of normal human skin with antiphospho-Ser211 GR antibody that recognizes ligand-induced phosphorylation at Ser211 ( ).

Plasmid Preparation:

Article Title: Four key steps control glycolytic flux in mammalian cells
Article Snippet: .. Plasmid DNA was prepared by the QIAprep Spin Miniprep Kit and constructs were sequence verified by Sanger sequencing (Genewiz, South Plainfield, NJ, USA). .. iBMK cells were transfected using Lipofectamine 2000 as described in the manufacturer’s protocol.

Software:

Article Title: Cortisol Synthesis in Epidermis Is Induced by IL-1 and Tissue Injury *
Article Snippet: Primers were then designed using Beacon Designer software, and the choice of primer was selected on basis of lowest self-dimer, hairpin, and cross-dimer energies. .. We confirmed primers by cloning PCR products with TOPO-TA One Shot Chemical Transformation, isolated DNA using the QIAprep Spin Miniprep kit, and sequenced the plasmids through Genewiz.

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    Genewiz qiaprep spin miniprep kit
    Qiaprep Spin Miniprep Kit, supplied by Genewiz, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaprep spin miniprep kit/product/Genewiz
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    qiaprep spin miniprep kit - by Bioz Stars, 2020-04
    93/100 stars
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