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Eton Bioscience qiaprep spin miniprep kit
Qiaprep Spin Miniprep Kit, supplied by Eton Bioscience, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/qiaprep spin miniprep kit/product/Eton Bioscience
Average 87 stars, based on 1 article reviews
Price from $9.99 to $1999.99
qiaprep spin miniprep kit - by Bioz Stars, 2020-04
87/100 stars

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Clone Assay:

Article Title: Epimerase (Msed_0639) and Mutase (Msed_0638 and Msed_2055) Convert (S)-Methylmalonyl-Coenzyme A (CoA) to Succinyl-CoA in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Cycle
Article Snippet: Paragraph title: Cloning, expression, and purification of MCE and MCM. ... Plasmids pET46-MCE, pET46-MCM-α, and pRSF-MCM(β) transformed into NovaBlue GigaSingles E. coli cells were extracted with a QIAprep Spin Miniprep kit and with the sequence confirmed by Eton Bioscience Inc. (Durham, NC).

Amplification:

Article Title: Epimerase (Msed_0639) and Mutase (Msed_0638 and Msed_2055) Convert (S)-Methylmalonyl-Coenzyme A (CoA) to Succinyl-CoA in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Cycle
Article Snippet: These genes ( mce , mcm -α, and mcm -β) were amplified using genomic DNA as the template (primers synthesized by Integrated DNA Technologies [Coralville, IA] are listed in Table S1 in the supplemental material). mce and mcm -α were ligated into pET46; mcm -β was ligated into pRSF-2 Ek/LIC, according to the protocol of an EK/LIC cloning kit. .. Plasmids pET46-MCE, pET46-MCM-α, and pRSF-MCM(β) transformed into NovaBlue GigaSingles E. coli cells were extracted with a QIAprep Spin Miniprep kit and with the sequence confirmed by Eton Bioscience Inc. (Durham, NC).

Binding Assay:

Article Title: Epimerase (Msed_0639) and Mutase (Msed_0638 and Msed_2055) Convert (S)-Methylmalonyl-Coenzyme A (CoA) to Succinyl-CoA in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Cycle
Article Snippet: Msed_0638 and Msed_2055 were predicted to be the catalytic subunit (MCM-α) and coenzyme B12 -binding subunit (MCM-β), respectively, of MCM, while Msed_0639 corresponded to MCE. .. Plasmids pET46-MCE, pET46-MCM-α, and pRSF-MCM(β) transformed into NovaBlue GigaSingles E. coli cells were extracted with a QIAprep Spin Miniprep kit and with the sequence confirmed by Eton Bioscience Inc. (Durham, NC).

Synthesized:

Article Title: Epimerase (Msed_0639) and Mutase (Msed_0638 and Msed_2055) Convert (S)-Methylmalonyl-Coenzyme A (CoA) to Succinyl-CoA in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Cycle
Article Snippet: These genes ( mce , mcm -α, and mcm -β) were amplified using genomic DNA as the template (primers synthesized by Integrated DNA Technologies [Coralville, IA] are listed in Table S1 in the supplemental material). mce and mcm -α were ligated into pET46; mcm -β was ligated into pRSF-2 Ek/LIC, according to the protocol of an EK/LIC cloning kit. .. Plasmids pET46-MCE, pET46-MCM-α, and pRSF-MCM(β) transformed into NovaBlue GigaSingles E. coli cells were extracted with a QIAprep Spin Miniprep kit and with the sequence confirmed by Eton Bioscience Inc. (Durham, NC).

Sequencing:

Article Title: Epimerase (Msed_0639) and Mutase (Msed_0638 and Msed_2055) Convert (S)-Methylmalonyl-Coenzyme A (CoA) to Succinyl-CoA in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Cycle
Article Snippet: .. Plasmids pET46-MCE, pET46-MCM-α, and pRSF-MCM(β) transformed into NovaBlue GigaSingles E. coli cells were extracted with a QIAprep Spin Miniprep kit and with the sequence confirmed by Eton Bioscience Inc. (Durham, NC). .. Plasmid pET46-MCE was transformed into E. coli Rosetta 2 (DE3) by heat shock and selected by overnight growth on Luria-Bertani (LB) agar medium supplemented with the two antibiotics (ampicillin, 100 μg/ml; chloramphenicol, 50 μg/ml) at 37°C.

Purification:

Article Title: Epimerase (Msed_0639) and Mutase (Msed_0638 and Msed_2055) Convert (S)-Methylmalonyl-Coenzyme A (CoA) to Succinyl-CoA in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Cycle
Article Snippet: Paragraph title: Cloning, expression, and purification of MCE and MCM. ... Plasmids pET46-MCE, pET46-MCM-α, and pRSF-MCM(β) transformed into NovaBlue GigaSingles E. coli cells were extracted with a QIAprep Spin Miniprep kit and with the sequence confirmed by Eton Bioscience Inc. (Durham, NC).

Produced:

Article Title: Epimerase (Msed_0639) and Mutase (Msed_0638 and Msed_2055) Convert (S)-Methylmalonyl-Coenzyme A (CoA) to Succinyl-CoA in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Cycle
Article Snippet: Plasmids pET46-MCE, pET46-MCM-α, and pRSF-MCM(β) transformed into NovaBlue GigaSingles E. coli cells were extracted with a QIAprep Spin Miniprep kit and with the sequence confirmed by Eton Bioscience Inc. (Durham, NC). .. Recombinant protein was produced and purified as reported elsewhere ( ) with minor modification.

IA:

Article Title: Epimerase (Msed_0639) and Mutase (Msed_0638 and Msed_2055) Convert (S)-Methylmalonyl-Coenzyme A (CoA) to Succinyl-CoA in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Cycle
Article Snippet: These genes ( mce , mcm -α, and mcm -β) were amplified using genomic DNA as the template (primers synthesized by Integrated DNA Technologies [Coralville, IA] are listed in Table S1 in the supplemental material). mce and mcm -α were ligated into pET46; mcm -β was ligated into pRSF-2 Ek/LIC, according to the protocol of an EK/LIC cloning kit. .. Plasmids pET46-MCE, pET46-MCM-α, and pRSF-MCM(β) transformed into NovaBlue GigaSingles E. coli cells were extracted with a QIAprep Spin Miniprep kit and with the sequence confirmed by Eton Bioscience Inc. (Durham, NC).

Expressing:

Article Title: Epimerase (Msed_0639) and Mutase (Msed_0638 and Msed_2055) Convert (S)-Methylmalonyl-Coenzyme A (CoA) to Succinyl-CoA in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Cycle
Article Snippet: Paragraph title: Cloning, expression, and purification of MCE and MCM. ... Plasmids pET46-MCE, pET46-MCM-α, and pRSF-MCM(β) transformed into NovaBlue GigaSingles E. coli cells were extracted with a QIAprep Spin Miniprep kit and with the sequence confirmed by Eton Bioscience Inc. (Durham, NC).

Modification:

Article Title: Epimerase (Msed_0639) and Mutase (Msed_0638 and Msed_2055) Convert (S)-Methylmalonyl-Coenzyme A (CoA) to Succinyl-CoA in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Cycle
Article Snippet: Plasmids pET46-MCE, pET46-MCM-α, and pRSF-MCM(β) transformed into NovaBlue GigaSingles E. coli cells were extracted with a QIAprep Spin Miniprep kit and with the sequence confirmed by Eton Bioscience Inc. (Durham, NC). .. Recombinant protein was produced and purified as reported elsewhere ( ) with minor modification.

Transformation Assay:

Article Title: Epimerase (Msed_0639) and Mutase (Msed_0638 and Msed_2055) Convert (S)-Methylmalonyl-Coenzyme A (CoA) to Succinyl-CoA in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Cycle
Article Snippet: .. Plasmids pET46-MCE, pET46-MCM-α, and pRSF-MCM(β) transformed into NovaBlue GigaSingles E. coli cells were extracted with a QIAprep Spin Miniprep kit and with the sequence confirmed by Eton Bioscience Inc. (Durham, NC). .. Plasmid pET46-MCE was transformed into E. coli Rosetta 2 (DE3) by heat shock and selected by overnight growth on Luria-Bertani (LB) agar medium supplemented with the two antibiotics (ampicillin, 100 μg/ml; chloramphenicol, 50 μg/ml) at 37°C.

Recombinant:

Article Title: Epimerase (Msed_0639) and Mutase (Msed_0638 and Msed_2055) Convert (S)-Methylmalonyl-Coenzyme A (CoA) to Succinyl-CoA in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Cycle
Article Snippet: Plasmids pET46-MCE, pET46-MCM-α, and pRSF-MCM(β) transformed into NovaBlue GigaSingles E. coli cells were extracted with a QIAprep Spin Miniprep kit and with the sequence confirmed by Eton Bioscience Inc. (Durham, NC). .. Recombinant protein was produced and purified as reported elsewhere ( ) with minor modification.

Plasmid Preparation:

Article Title: Epimerase (Msed_0639) and Mutase (Msed_0638 and Msed_2055) Convert (S)-Methylmalonyl-Coenzyme A (CoA) to Succinyl-CoA in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Cycle
Article Snippet: Plasmids pET46-MCE, pET46-MCM-α, and pRSF-MCM(β) transformed into NovaBlue GigaSingles E. coli cells were extracted with a QIAprep Spin Miniprep kit and with the sequence confirmed by Eton Bioscience Inc. (Durham, NC). .. Plasmid pET46-MCE was transformed into E. coli Rosetta 2 (DE3) by heat shock and selected by overnight growth on Luria-Bertani (LB) agar medium supplemented with the two antibiotics (ampicillin, 100 μg/ml; chloramphenicol, 50 μg/ml) at 37°C.

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    Eton Bioscience qiaprep spin miniprep kit
    Qiaprep Spin Miniprep Kit, supplied by Eton Bioscience, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaprep spin miniprep kit/product/Eton Bioscience
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qiaprep spin miniprep kit - by Bioz Stars, 2020-04
    87/100 stars
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