qiaamp rneasy minikit  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92

    Structured Review

    Qiagen qiaamp rneasy minikit
    Qiaamp Rneasy Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaamp rneasy minikit/product/Qiagen
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    qiaamp rneasy minikit - by Bioz Stars, 2020-04
    92/100 stars

    Images

    Related Articles

    Diagnostic Assay:

    Article Title: Hobi-Like Pestivirus in Aborted Bovine Fetuses
    Article Snippet: Two aborted fetuses (280/11-A, 280/11-B) were sent to our laboratory, and tissue samples were collected from lungs, spleens, livers, kidneys, and placentas for diagnostic investigations. .. Nucleic acids were purified using the DNeasy tissue kit (Qiagen) and QIAamp RNeasy minikit (Qiagen).

    Amplification:

    Article Title: A Phase I Clinical Trial of Ad5/3-Δ24, a Novel Serotype-Chimeric, Infectivity-Enhanced, Conditionally-Replicative Adenovirus (CRAd), in Patients with Recurrent Ovarian Cancer
    Article Snippet: Ad5/3-Δ24 replication was evaluated by comparison of E1A copies as detected in the specimens of total RNA, isolated from ascites samples using the QIAamp RNeasy MiniKit (QIAGEN, Valencia, CA) and used as a template for quantitative reverse transcription PCR (RT-PCR). .. Generation of wildtype adenovirus was examined with LightCycler methodology for amplification of the Ad5 E1 genome without Δ24 deletion by forward and reverse primers: 5’-TGC-CAA-ACC-TTG-TAC-CGG-A-3’ and 5’-CGT-CGT-CAC-TGG-GTG-GAA-A-3’, and probe 6-FAM-ATC-GAT-CTT-ACC-TGC-CAC-GAG-GCT-GG-BHQ-1.

    Stable Transfection:

    Article Title: Sangassou Virus, the First Hantavirus Isolate from Africa, Displays Genetic and Functional Properties Distinct from Those of Other Murinae-Associated Hantaviruses
    Article Snippet: CHO and stably transfected CHO cells stably expressing β3 integrins (CHO-β3 cells) were grown until confluence and cooled down to 4°C. .. After incubation, the cells were washed five times with cold medium, and bound virus was lysed by RLT buffer from QIAamp RNeasy minikit (Qiagen) and used for RNA extraction according to the manufacturer's specifications.

    Article Title: Dobrava-Belgrade Hantavirus from Germany Shows Receptor Usage and Innate Immunity Induction Consistent with the Pathogenicity of the Virus in Humans
    Article Snippet: Receptor binding experiments CHO and stably transfected CHO cells stably expressing β3 integrins (CHO-β3 cells) were grown until confluence and cooled down to 4°C. .. After incubation, the cells were washed five times with cold medium, bound virus was lysed by RLT buffer from QIAamp RNeasy minikit (Qiagen, Germany) and used for RNA extraction according to the manufacturer's specifications.

    Quantitative RT-PCR:

    Article Title: Sangassou Virus, the First Hantavirus Isolate from Africa, Displays Genetic and Functional Properties Distinct from Those of Other Murinae-Associated Hantaviruses
    Article Snippet: After incubation, the cells were washed five times with cold medium, and bound virus was lysed by RLT buffer from QIAamp RNeasy minikit (Qiagen) and used for RNA extraction according to the manufacturer's specifications. .. The binding affinity of virus particles to CHO-β3 cells was measured by RT-qPCR as a ratio between the number of virus genome equivalents detected on CHO-β3 cells in comparison to those detected on nontransfected CHO cells.

    Article Title: Detection of Viruses in Human Adenoid Tissues by Use of Multiplex PCR ▿
    Article Snippet: Approximately 25 mg of each adenoid tissue specimen was used for DNA and RNA extraction using a QIAamp DNeasy tissue kit and a QIAamp RNeasy minikit (Qiagen, Valencia, CA), respectively. .. RNA was also tested by real-time RT-PCR and conventional RT-PCR to detect human coronaviruses (hCoVs) and reoviruses (ReoVs), respectively, as previously described ( , , ).

    Article Title: Establishment of cell lines with increased susceptibility to EV71/CA16 by stable overexpression of SCARB2
    Article Snippet: .. Real-time RT-PCR To detect the relative SCARB2 expression at the gene level, mRNA was extracted from the six cell lines with the QIAamp RNeasy MiniKit separately according to the manufacturer’s instructions (QIAGEN). .. Reverse transcription was performed with TAKARA Reverse Transcriptase XL.

    Article Title: Differential Effects of Drugs Targeting Cancer Stem Cell (CSC) and Non-CSC Populations on Lung Primary Tumors and Metastasis
    Article Snippet: .. RNA Extraction and Quantitative Real Time PCR (qRT-PCR) Total RNA was isolated from cells, sphere-containing pellets, or frozen tissue samples using QIAamp RNeasy Minikit (Qiagen, Chatsworth, CA). .. After DNase I treatment, reverse transcription was performed with Superscript II reverse transcriptase (Invitrogen, Carlsbad, CA) to generate complementary DNA (cDNA). qRT-PCR was run in an Applied Biosystems 7900 Real-time PCR machine. qRT-PCR reactions were carried out with SYBR Green PCR Master Mix (Applied Biosystems, Forster City, CA, USA) and GAPDH levels were used as controls.

    SYBR Green Assay:

    Article Title: Human Mucosal Mast Cells Capture HIV-1 and Mediate Viral trans-Infection of CD4+ T Cells
    Article Snippet: Total cellular DNA or RNA was extracted by use of a QIAamp DNA minikit or QIAamp RNeasy minikit (Qiagen). .. The products were semiquantified by use of SYBR green I and normalized to β-actin.

    Article Title: Differential Effects of Drugs Targeting Cancer Stem Cell (CSC) and Non-CSC Populations on Lung Primary Tumors and Metastasis
    Article Snippet: RNA Extraction and Quantitative Real Time PCR (qRT-PCR) Total RNA was isolated from cells, sphere-containing pellets, or frozen tissue samples using QIAamp RNeasy Minikit (Qiagen, Chatsworth, CA). .. After DNase I treatment, reverse transcription was performed with Superscript II reverse transcriptase (Invitrogen, Carlsbad, CA) to generate complementary DNA (cDNA). qRT-PCR was run in an Applied Biosystems 7900 Real-time PCR machine. qRT-PCR reactions were carried out with SYBR Green PCR Master Mix (Applied Biosystems, Forster City, CA, USA) and GAPDH levels were used as controls.

    Incubation:

    Article Title: Sangassou Virus, the First Hantavirus Isolate from Africa, Displays Genetic and Functional Properties Distinct from Those of Other Murinae-Associated Hantaviruses
    Article Snippet: .. After incubation, the cells were washed five times with cold medium, and bound virus was lysed by RLT buffer from QIAamp RNeasy minikit (Qiagen) and used for RNA extraction according to the manufacturer's specifications. .. The binding affinity of virus particles to CHO-β3 cells was measured by RT-qPCR as a ratio between the number of virus genome equivalents detected on CHO-β3 cells in comparison to those detected on nontransfected CHO cells.

    Article Title: Dobrava-Belgrade Hantavirus from Germany Shows Receptor Usage and Innate Immunity Induction Consistent with the Pathogenicity of the Virus in Humans
    Article Snippet: .. After incubation, the cells were washed five times with cold medium, bound virus was lysed by RLT buffer from QIAamp RNeasy minikit (Qiagen, Germany) and used for RNA extraction according to the manufacturer's specifications. .. The binding affinity of virus particles to CHO-β3 cells was measured by qPCR as a ratio between the number of virus genome equivalents detected on CHO-β3 cells in comparison to those detected on CHO cells.

    Infection:

    Article Title: Naf1 Regulates HIV-1 Latency by Suppressing Viral Promoter-Driven Gene Expression in Primary CD4+ T Cells
    Article Snippet: Purified resting CD4+ T cells (1 × 106 /sample) isolated from HIV-1-infected individuals who had undergone combination antiretroviral therapy were coinfected with lentiviruses containing Naf1 shRNA or the scramble control (50 ng p24gag ) and lentiviruses containing Vpx (multiplicity of infection [MOI] = 0.5) for 6 h , and after washing, the cells were cultured for another 3 days. .. For RT-PCR, total cellular RNA was extracted by use of a QIAamp RNeasy minikit (Qiagen), and the HIV-1 gag or tat-rev mRNA was quantified and normalized to the GAPDH mRNA level.

    Article Title: Human Mucosal Mast Cells Capture HIV-1 and Mediate Viral trans-Infection of CD4+ T Cells
    Article Snippet: Paragraph title: HIV-1 infection assay. ... Total cellular DNA or RNA was extracted by use of a QIAamp DNA minikit or QIAamp RNeasy minikit (Qiagen).

    Article Title: A Phase I Clinical Trial of Ad5/3-Δ24, a Novel Serotype-Chimeric, Infectivity-Enhanced, Conditionally-Replicative Adenovirus (CRAd), in Patients with Recurrent Ovarian Cancer
    Article Snippet: Paragraph title: Assessment of Cellular Infection, Viral Replication and Generation of WildType Adenovirus in Ascites ... Ad5/3-Δ24 replication was evaluated by comparison of E1A copies as detected in the specimens of total RNA, isolated from ascites samples using the QIAamp RNeasy MiniKit (QIAGEN, Valencia, CA) and used as a template for quantitative reverse transcription PCR (RT-PCR).

    Expressing:

    Article Title: Sangassou Virus, the First Hantavirus Isolate from Africa, Displays Genetic and Functional Properties Distinct from Those of Other Murinae-Associated Hantaviruses
    Article Snippet: CHO and stably transfected CHO cells stably expressing β3 integrins (CHO-β3 cells) were grown until confluence and cooled down to 4°C. .. After incubation, the cells were washed five times with cold medium, and bound virus was lysed by RLT buffer from QIAamp RNeasy minikit (Qiagen) and used for RNA extraction according to the manufacturer's specifications.

    Article Title: Dobrava-Belgrade Hantavirus from Germany Shows Receptor Usage and Innate Immunity Induction Consistent with the Pathogenicity of the Virus in Humans
    Article Snippet: Receptor binding experiments CHO and stably transfected CHO cells stably expressing β3 integrins (CHO-β3 cells) were grown until confluence and cooled down to 4°C. .. After incubation, the cells were washed five times with cold medium, bound virus was lysed by RLT buffer from QIAamp RNeasy minikit (Qiagen, Germany) and used for RNA extraction according to the manufacturer's specifications.

    Article Title: Establishment of cell lines with increased susceptibility to EV71/CA16 by stable overexpression of SCARB2
    Article Snippet: .. Real-time RT-PCR To detect the relative SCARB2 expression at the gene level, mRNA was extracted from the six cell lines with the QIAamp RNeasy MiniKit separately according to the manufacturer’s instructions (QIAGEN). .. Reverse transcription was performed with TAKARA Reverse Transcriptase XL.

    Article Title: Differential Effects of Drugs Targeting Cancer Stem Cell (CSC) and Non-CSC Populations on Lung Primary Tumors and Metastasis
    Article Snippet: RNA Extraction and Quantitative Real Time PCR (qRT-PCR) Total RNA was isolated from cells, sphere-containing pellets, or frozen tissue samples using QIAamp RNeasy Minikit (Qiagen, Chatsworth, CA). .. The mean cycle threshold value (Ct) for the gene of interest, normalized to the Ct value of the housekeeping gene (GAPDH) was used to calculate gene expression values.

    Transfection:

    Article Title: Sangassou Virus, the First Hantavirus Isolate from Africa, Displays Genetic and Functional Properties Distinct from Those of Other Murinae-Associated Hantaviruses
    Article Snippet: CHO and stably transfected CHO cells stably expressing β3 integrins (CHO-β3 cells) were grown until confluence and cooled down to 4°C. .. After incubation, the cells were washed five times with cold medium, and bound virus was lysed by RLT buffer from QIAamp RNeasy minikit (Qiagen) and used for RNA extraction according to the manufacturer's specifications.

    Article Title: Dobrava-Belgrade Hantavirus from Germany Shows Receptor Usage and Innate Immunity Induction Consistent with the Pathogenicity of the Virus in Humans
    Article Snippet: Receptor binding experiments CHO and stably transfected CHO cells stably expressing β3 integrins (CHO-β3 cells) were grown until confluence and cooled down to 4°C. .. After incubation, the cells were washed five times with cold medium, bound virus was lysed by RLT buffer from QIAamp RNeasy minikit (Qiagen, Germany) and used for RNA extraction according to the manufacturer's specifications.

    Concentration Assay:

    Article Title: A Phase I Clinical Trial of Ad5/3-Δ24, a Novel Serotype-Chimeric, Infectivity-Enhanced, Conditionally-Replicative Adenovirus (CRAd), in Patients with Recurrent Ovarian Cancer
    Article Snippet: Ad5/3-Δ24 genome copies present in each peritoneal aspirate sample were determined by Real-Time PCR (LightCycler system, Roche Molecular Biochemicals, Indianapolis, IN) with the Ad5 hexon gene-specific forward primer 5’-TAC-GCA-CGA-CGT-GAC-CAC-A-3’, reverse primer 5’-ATC-CTC-ACG-GTC-CAC-AGG-G-3’, and probe 6-FAM-ACC-GGT-CCC-AGC-GTT-TGA-CGC-BHQ-1 and normalized to the concentration of genomic DNA by amplifying the human β-actin housekeeping gene with forward primer, reverse primer and probe: 5’-CCA-GCA-GAT-GTG-GAT-CAG-CA-3’, 5’-CTA-GAA-GCA-TTT-GCG-GTG-GAC-3’ and 6-HEX-AGG-AGT-ATG-ACG-AGT-CCG-GCC-CCT-C-BHQ-1, respectively. .. Ad5/3-Δ24 replication was evaluated by comparison of E1A copies as detected in the specimens of total RNA, isolated from ascites samples using the QIAamp RNeasy MiniKit (QIAGEN, Valencia, CA) and used as a template for quantitative reverse transcription PCR (RT-PCR).

    Cell Culture:

    Article Title: Naf1 Regulates HIV-1 Latency by Suppressing Viral Promoter-Driven Gene Expression in Primary CD4+ T Cells
    Article Snippet: Purified resting CD4+ T cells (1 × 106 /sample) isolated from HIV-1-infected individuals who had undergone combination antiretroviral therapy were coinfected with lentiviruses containing Naf1 shRNA or the scramble control (50 ng p24gag ) and lentiviruses containing Vpx (multiplicity of infection [MOI] = 0.5) for 6 h , and after washing, the cells were cultured for another 3 days. .. For RT-PCR, total cellular RNA was extracted by use of a QIAamp RNeasy minikit (Qiagen), and the HIV-1 gag or tat-rev mRNA was quantified and normalized to the GAPDH mRNA level.

    Article Title: Human Mucosal Mast Cells Capture HIV-1 and Mediate Viral trans-Infection of CD4+ T Cells
    Article Snippet: FcεR1+ mast cells, MDDCs, or PHA-P-activated PBLs were inoculated with wild-type HIV AD8 or HIV NL4-3 (5 ng of p24gag ) for 2 h and then washed and further cultured for the indicated time. .. Total cellular DNA or RNA was extracted by use of a QIAamp DNA minikit or QIAamp RNeasy minikit (Qiagen).

    Polymerase Chain Reaction:

    Article Title: Detection of Viruses in Human Adenoid Tissues by Use of Multiplex PCR ▿
    Article Snippet: To clarify this matter, we performed PCR to detect viruses in adenoid tissues removed by adenoidectomy. .. Approximately 25 mg of each adenoid tissue specimen was used for DNA and RNA extraction using a QIAamp DNeasy tissue kit and a QIAamp RNeasy minikit (Qiagen, Valencia, CA), respectively.

    Article Title: Establishment of cell lines with increased susceptibility to EV71/CA16 by stable overexpression of SCARB2
    Article Snippet: Real-time RT-PCR To detect the relative SCARB2 expression at the gene level, mRNA was extracted from the six cell lines with the QIAamp RNeasy MiniKit separately according to the manufacturer’s instructions (QIAGEN). .. PCR was carried out using the following primers: SF: 5′-GTACTGAGGCATTTGACTCCT-3′, SR: 5′-AGTTCCCTGTAGGTGTATGGC-3′.

    Article Title: Differential Effects of Drugs Targeting Cancer Stem Cell (CSC) and Non-CSC Populations on Lung Primary Tumors and Metastasis
    Article Snippet: RNA Extraction and Quantitative Real Time PCR (qRT-PCR) Total RNA was isolated from cells, sphere-containing pellets, or frozen tissue samples using QIAamp RNeasy Minikit (Qiagen, Chatsworth, CA). .. After DNase I treatment, reverse transcription was performed with Superscript II reverse transcriptase (Invitrogen, Carlsbad, CA) to generate complementary DNA (cDNA). qRT-PCR was run in an Applied Biosystems 7900 Real-time PCR machine. qRT-PCR reactions were carried out with SYBR Green PCR Master Mix (Applied Biosystems, Forster City, CA, USA) and GAPDH levels were used as controls.

    Article Title: A Phase I Clinical Trial of Ad5/3-Δ24, a Novel Serotype-Chimeric, Infectivity-Enhanced, Conditionally-Replicative Adenovirus (CRAd), in Patients with Recurrent Ovarian Cancer
    Article Snippet: .. Ad5/3-Δ24 replication was evaluated by comparison of E1A copies as detected in the specimens of total RNA, isolated from ascites samples using the QIAamp RNeasy MiniKit (QIAGEN, Valencia, CA) and used as a template for quantitative reverse transcription PCR (RT-PCR). .. Duplexing RT-PCR was carried out using the TaqMan one step PCR master mix supplemented with RT enzyme (Applied Biosystems) and primers and probes specific for the E1A target gene (forward primer 5’-AAC-CAG-TTG-CCG-TGA-GAG-TTG-3’, reverse primer 5’-CTC-GTT-AAG-CAA-GTC-CTC-GAT-ACA-3’, probe 6-FAM-CAC-AGC-CTG-GCG-ACG-CCC-A-BHQ-1) and the human β-actin housekeeping gene (above).

    Article Title: Hobi-Like Pestivirus in Aborted Bovine Fetuses
    Article Snippet: Nucleic acids were purified using the DNeasy tissue kit (Qiagen) and QIAamp RNeasy minikit (Qiagen). .. Reverse transcription (RT)-PCR and PCR assays were performed using SuperScript one-step RT-PCR for long templates (Life Technologies) and LA PCR kit version 2.1 (TaKaRa Bio Inc.), respectively.

    Binding Assay:

    Article Title: Sangassou Virus, the First Hantavirus Isolate from Africa, Displays Genetic and Functional Properties Distinct from Those of Other Murinae-Associated Hantaviruses
    Article Snippet: Paragraph title: Receptor binding experiments. ... After incubation, the cells were washed five times with cold medium, and bound virus was lysed by RLT buffer from QIAamp RNeasy minikit (Qiagen) and used for RNA extraction according to the manufacturer's specifications.

    Article Title: Dobrava-Belgrade Hantavirus from Germany Shows Receptor Usage and Innate Immunity Induction Consistent with the Pathogenicity of the Virus in Humans
    Article Snippet: Paragraph title: Receptor binding experiments ... After incubation, the cells were washed five times with cold medium, bound virus was lysed by RLT buffer from QIAamp RNeasy minikit (Qiagen, Germany) and used for RNA extraction according to the manufacturer's specifications.

    Isolation:

    Article Title: Naf1 Regulates HIV-1 Latency by Suppressing Viral Promoter-Driven Gene Expression in Primary CD4+ T Cells
    Article Snippet: Purified resting CD4+ T cells (1 × 106 /sample) isolated from HIV-1-infected individuals who had undergone combination antiretroviral therapy were coinfected with lentiviruses containing Naf1 shRNA or the scramble control (50 ng p24gag ) and lentiviruses containing Vpx (multiplicity of infection [MOI] = 0.5) for 6 h , and after washing, the cells were cultured for another 3 days. .. For RT-PCR, total cellular RNA was extracted by use of a QIAamp RNeasy minikit (Qiagen), and the HIV-1 gag or tat-rev mRNA was quantified and normalized to the GAPDH mRNA level.

    Article Title: Differential Effects of Drugs Targeting Cancer Stem Cell (CSC) and Non-CSC Populations on Lung Primary Tumors and Metastasis
    Article Snippet: .. RNA Extraction and Quantitative Real Time PCR (qRT-PCR) Total RNA was isolated from cells, sphere-containing pellets, or frozen tissue samples using QIAamp RNeasy Minikit (Qiagen, Chatsworth, CA). .. After DNase I treatment, reverse transcription was performed with Superscript II reverse transcriptase (Invitrogen, Carlsbad, CA) to generate complementary DNA (cDNA). qRT-PCR was run in an Applied Biosystems 7900 Real-time PCR machine. qRT-PCR reactions were carried out with SYBR Green PCR Master Mix (Applied Biosystems, Forster City, CA, USA) and GAPDH levels were used as controls.

    Article Title: A Phase I Clinical Trial of Ad5/3-Δ24, a Novel Serotype-Chimeric, Infectivity-Enhanced, Conditionally-Replicative Adenovirus (CRAd), in Patients with Recurrent Ovarian Cancer
    Article Snippet: .. Ad5/3-Δ24 replication was evaluated by comparison of E1A copies as detected in the specimens of total RNA, isolated from ascites samples using the QIAamp RNeasy MiniKit (QIAGEN, Valencia, CA) and used as a template for quantitative reverse transcription PCR (RT-PCR). .. Duplexing RT-PCR was carried out using the TaqMan one step PCR master mix supplemented with RT enzyme (Applied Biosystems) and primers and probes specific for the E1A target gene (forward primer 5’-AAC-CAG-TTG-CCG-TGA-GAG-TTG-3’, reverse primer 5’-CTC-GTT-AAG-CAA-GTC-CTC-GAT-ACA-3’, probe 6-FAM-CAC-AGC-CTG-GCG-ACG-CCC-A-BHQ-1) and the human β-actin housekeeping gene (above).

    Article Title: Hobi-Like Pestivirus in Aborted Bovine Fetuses
    Article Snippet: Here, we report the isolation and genetic characterization of a Hobi-like strain detected from aborted fetuses in southern Italy. .. Nucleic acids were purified using the DNeasy tissue kit (Qiagen) and QIAamp RNeasy minikit (Qiagen).

    RNA Extraction:

    Article Title: Sangassou Virus, the First Hantavirus Isolate from Africa, Displays Genetic and Functional Properties Distinct from Those of Other Murinae-Associated Hantaviruses
    Article Snippet: .. After incubation, the cells were washed five times with cold medium, and bound virus was lysed by RLT buffer from QIAamp RNeasy minikit (Qiagen) and used for RNA extraction according to the manufacturer's specifications. .. The binding affinity of virus particles to CHO-β3 cells was measured by RT-qPCR as a ratio between the number of virus genome equivalents detected on CHO-β3 cells in comparison to those detected on nontransfected CHO cells.

    Article Title: Dobrava-Belgrade Hantavirus from Germany Shows Receptor Usage and Innate Immunity Induction Consistent with the Pathogenicity of the Virus in Humans
    Article Snippet: .. After incubation, the cells were washed five times with cold medium, bound virus was lysed by RLT buffer from QIAamp RNeasy minikit (Qiagen, Germany) and used for RNA extraction according to the manufacturer's specifications. .. The binding affinity of virus particles to CHO-β3 cells was measured by qPCR as a ratio between the number of virus genome equivalents detected on CHO-β3 cells in comparison to those detected on CHO cells.

    Article Title: Detection of Viruses in Human Adenoid Tissues by Use of Multiplex PCR ▿
    Article Snippet: .. Approximately 25 mg of each adenoid tissue specimen was used for DNA and RNA extraction using a QIAamp DNeasy tissue kit and a QIAamp RNeasy minikit (Qiagen, Valencia, CA), respectively. .. Eight monoplex PCR-enzyme immunoassays were used to detect herpes simplex virus (HSV), CMV, EBV, varicella-zoster virus (VZV), HHV-6, HHV-7, HHV-8, and ADVs, and a multiplex reverse transcriptase (RT)-PCR performed as previously described ( , , ) was used to amplify influenza A virus (FLUAV), FLUBV, parainfluenza virus 1 (PIV-1), PIV-2, PIV-3, PIV-4, respiratory syncytial virus A (RSV-A), RSV-B, human metapneumovirus (hMPV), rhinoviruses (RhVs), and enteroviruses (EnVs) in a single reaction.

    Article Title: Differential Effects of Drugs Targeting Cancer Stem Cell (CSC) and Non-CSC Populations on Lung Primary Tumors and Metastasis
    Article Snippet: .. RNA Extraction and Quantitative Real Time PCR (qRT-PCR) Total RNA was isolated from cells, sphere-containing pellets, or frozen tissue samples using QIAamp RNeasy Minikit (Qiagen, Chatsworth, CA). .. After DNase I treatment, reverse transcription was performed with Superscript II reverse transcriptase (Invitrogen, Carlsbad, CA) to generate complementary DNA (cDNA). qRT-PCR was run in an Applied Biosystems 7900 Real-time PCR machine. qRT-PCR reactions were carried out with SYBR Green PCR Master Mix (Applied Biosystems, Forster City, CA, USA) and GAPDH levels were used as controls.

    Purification:

    Article Title: Naf1 Regulates HIV-1 Latency by Suppressing Viral Promoter-Driven Gene Expression in Primary CD4+ T Cells
    Article Snippet: Purified resting CD4+ T cells (1 × 106 /sample) isolated from HIV-1-infected individuals who had undergone combination antiretroviral therapy were coinfected with lentiviruses containing Naf1 shRNA or the scramble control (50 ng p24gag ) and lentiviruses containing Vpx (multiplicity of infection [MOI] = 0.5) for 6 h , and after washing, the cells were cultured for another 3 days. .. For RT-PCR, total cellular RNA was extracted by use of a QIAamp RNeasy minikit (Qiagen), and the HIV-1 gag or tat-rev mRNA was quantified and normalized to the GAPDH mRNA level.

    Article Title: Hobi-Like Pestivirus in Aborted Bovine Fetuses
    Article Snippet: .. Nucleic acids were purified using the DNeasy tissue kit (Qiagen) and QIAamp RNeasy minikit (Qiagen). .. Reverse transcription (RT)-PCR and PCR assays were performed using SuperScript one-step RT-PCR for long templates (Life Technologies) and LA PCR kit version 2.1 (TaKaRa Bio Inc.), respectively.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Naf1 Regulates HIV-1 Latency by Suppressing Viral Promoter-Driven Gene Expression in Primary CD4+ T Cells
    Article Snippet: .. For RT-PCR, total cellular RNA was extracted by use of a QIAamp RNeasy minikit (Qiagen), and the HIV-1 gag or tat-rev mRNA was quantified and normalized to the GAPDH mRNA level. .. The primer sequences were as follows: for gag , forward primer 5′-GTG TGG AAA ATC TCT AGC AGT GG-3′ and reverse primer 5′-CGC TCT CGC ACC CAT CTC-3′; for tat-rev , forward primer 5′-ATG GCA GGA AGA AGC GGA G-3′ and reverse primer 5′-ATT CCT TCG GGC CTG TCG-3′; and for GAPDH, forward primer 5′-ATC CCA TCA CCA TCT TCC AGG-3′ and reverse primer 5′-CCT TCT CCA TGG TGG TGA AGA C-3′.

    Article Title: Detection of Viruses in Human Adenoid Tissues by Use of Multiplex PCR ▿
    Article Snippet: Approximately 25 mg of each adenoid tissue specimen was used for DNA and RNA extraction using a QIAamp DNeasy tissue kit and a QIAamp RNeasy minikit (Qiagen, Valencia, CA), respectively. .. Eight monoplex PCR-enzyme immunoassays were used to detect herpes simplex virus (HSV), CMV, EBV, varicella-zoster virus (VZV), HHV-6, HHV-7, HHV-8, and ADVs, and a multiplex reverse transcriptase (RT)-PCR performed as previously described ( , , ) was used to amplify influenza A virus (FLUAV), FLUBV, parainfluenza virus 1 (PIV-1), PIV-2, PIV-3, PIV-4, respiratory syncytial virus A (RSV-A), RSV-B, human metapneumovirus (hMPV), rhinoviruses (RhVs), and enteroviruses (EnVs) in a single reaction.

    Article Title: A Phase I Clinical Trial of Ad5/3-Δ24, a Novel Serotype-Chimeric, Infectivity-Enhanced, Conditionally-Replicative Adenovirus (CRAd), in Patients with Recurrent Ovarian Cancer
    Article Snippet: .. Ad5/3-Δ24 replication was evaluated by comparison of E1A copies as detected in the specimens of total RNA, isolated from ascites samples using the QIAamp RNeasy MiniKit (QIAGEN, Valencia, CA) and used as a template for quantitative reverse transcription PCR (RT-PCR). .. Duplexing RT-PCR was carried out using the TaqMan one step PCR master mix supplemented with RT enzyme (Applied Biosystems) and primers and probes specific for the E1A target gene (forward primer 5’-AAC-CAG-TTG-CCG-TGA-GAG-TTG-3’, reverse primer 5’-CTC-GTT-AAG-CAA-GTC-CTC-GAT-ACA-3’, probe 6-FAM-CAC-AGC-CTG-GCG-ACG-CCC-A-BHQ-1) and the human β-actin housekeeping gene (above).

    Article Title: Hobi-Like Pestivirus in Aborted Bovine Fetuses
    Article Snippet: Nucleic acids were purified using the DNeasy tissue kit (Qiagen) and QIAamp RNeasy minikit (Qiagen). .. Reverse transcription (RT)-PCR and PCR assays were performed using SuperScript one-step RT-PCR for long templates (Life Technologies) and LA PCR kit version 2.1 (TaKaRa Bio Inc.), respectively.

    Nested PCR:

    Article Title: Hobi-Like Pestivirus in Aborted Bovine Fetuses
    Article Snippet: Nucleic acids were purified using the DNeasy tissue kit (Qiagen) and QIAamp RNeasy minikit (Qiagen). .. Conversely, the pestivirus genome was detected with two different RT-PCR protocols ( , ), and the virus was characterized as Hobi-like by a species-specific nested PCR ( ) ( ).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Naf1 Regulates HIV-1 Latency by Suppressing Viral Promoter-Driven Gene Expression in Primary CD4+ T Cells
    Article Snippet: For RT-PCR, total cellular RNA was extracted by use of a QIAamp RNeasy minikit (Qiagen), and the HIV-1 gag or tat-rev mRNA was quantified and normalized to the GAPDH mRNA level. .. The primer sequences were as follows: for gag , forward primer 5′-GTG TGG AAA ATC TCT AGC AGT GG-3′ and reverse primer 5′-CGC TCT CGC ACC CAT CTC-3′; for tat-rev , forward primer 5′-ATG GCA GGA AGA AGC GGA G-3′ and reverse primer 5′-ATT CCT TCG GGC CTG TCG-3′; and for GAPDH, forward primer 5′-ATC CCA TCA CCA TCT TCC AGG-3′ and reverse primer 5′-CCT TCT CCA TGG TGG TGA AGA C-3′.

    Software:

    Article Title: A Phase I Clinical Trial of Ad5/3-Δ24, a Novel Serotype-Chimeric, Infectivity-Enhanced, Conditionally-Replicative Adenovirus (CRAd), in Patients with Recurrent Ovarian Cancer
    Article Snippet: Ad5/3-Δ24 replication was evaluated by comparison of E1A copies as detected in the specimens of total RNA, isolated from ascites samples using the QIAamp RNeasy MiniKit (QIAGEN, Valencia, CA) and used as a template for quantitative reverse transcription PCR (RT-PCR). .. Data was analyzed with LightCycler 480 1.5.0 SP1 software and the resultant E1A copies were normalized to the amount of human β-actin detected in the same sample to allow for comparison between patients and at different time points.

    Real-time Polymerase Chain Reaction:

    Article Title: Dobrava-Belgrade Hantavirus from Germany Shows Receptor Usage and Innate Immunity Induction Consistent with the Pathogenicity of the Virus in Humans
    Article Snippet: After incubation, the cells were washed five times with cold medium, bound virus was lysed by RLT buffer from QIAamp RNeasy minikit (Qiagen, Germany) and used for RNA extraction according to the manufacturer's specifications. .. The binding affinity of virus particles to CHO-β3 cells was measured by qPCR as a ratio between the number of virus genome equivalents detected on CHO-β3 cells in comparison to those detected on CHO cells.

    Article Title: Human Mucosal Mast Cells Capture HIV-1 and Mediate Viral trans-Infection of CD4+ T Cells
    Article Snippet: HIV-1 infection was determined mainly by real-time PCR for quantification of viral replication products. .. Total cellular DNA or RNA was extracted by use of a QIAamp DNA minikit or QIAamp RNeasy minikit (Qiagen).

    Article Title: Differential Effects of Drugs Targeting Cancer Stem Cell (CSC) and Non-CSC Populations on Lung Primary Tumors and Metastasis
    Article Snippet: .. RNA Extraction and Quantitative Real Time PCR (qRT-PCR) Total RNA was isolated from cells, sphere-containing pellets, or frozen tissue samples using QIAamp RNeasy Minikit (Qiagen, Chatsworth, CA). .. After DNase I treatment, reverse transcription was performed with Superscript II reverse transcriptase (Invitrogen, Carlsbad, CA) to generate complementary DNA (cDNA). qRT-PCR was run in an Applied Biosystems 7900 Real-time PCR machine. qRT-PCR reactions were carried out with SYBR Green PCR Master Mix (Applied Biosystems, Forster City, CA, USA) and GAPDH levels were used as controls.

    Article Title: A Phase I Clinical Trial of Ad5/3-Δ24, a Novel Serotype-Chimeric, Infectivity-Enhanced, Conditionally-Replicative Adenovirus (CRAd), in Patients with Recurrent Ovarian Cancer
    Article Snippet: Ad5/3-Δ24 genome copies present in each peritoneal aspirate sample were determined by Real-Time PCR (LightCycler system, Roche Molecular Biochemicals, Indianapolis, IN) with the Ad5 hexon gene-specific forward primer 5’-TAC-GCA-CGA-CGT-GAC-CAC-A-3’, reverse primer 5’-ATC-CTC-ACG-GTC-CAC-AGG-G-3’, and probe 6-FAM-ACC-GGT-CCC-AGC-GTT-TGA-CGC-BHQ-1 and normalized to the concentration of genomic DNA by amplifying the human β-actin housekeeping gene with forward primer, reverse primer and probe: 5’-CCA-GCA-GAT-GTG-GAT-CAG-CA-3’, 5’-CTA-GAA-GCA-TTT-GCG-GTG-GAC-3’ and 6-HEX-AGG-AGT-ATG-ACG-AGT-CCG-GCC-CCT-C-BHQ-1, respectively. .. Ad5/3-Δ24 replication was evaluated by comparison of E1A copies as detected in the specimens of total RNA, isolated from ascites samples using the QIAamp RNeasy MiniKit (QIAGEN, Valencia, CA) and used as a template for quantitative reverse transcription PCR (RT-PCR).

    Multiplex Assay:

    Article Title: Detection of Viruses in Human Adenoid Tissues by Use of Multiplex PCR ▿
    Article Snippet: Approximately 25 mg of each adenoid tissue specimen was used for DNA and RNA extraction using a QIAamp DNeasy tissue kit and a QIAamp RNeasy minikit (Qiagen, Valencia, CA), respectively. .. Eight monoplex PCR-enzyme immunoassays were used to detect herpes simplex virus (HSV), CMV, EBV, varicella-zoster virus (VZV), HHV-6, HHV-7, HHV-8, and ADVs, and a multiplex reverse transcriptase (RT)-PCR performed as previously described ( , , ) was used to amplify influenza A virus (FLUAV), FLUBV, parainfluenza virus 1 (PIV-1), PIV-2, PIV-3, PIV-4, respiratory syncytial virus A (RSV-A), RSV-B, human metapneumovirus (hMPV), rhinoviruses (RhVs), and enteroviruses (EnVs) in a single reaction.

    shRNA:

    Article Title: Naf1 Regulates HIV-1 Latency by Suppressing Viral Promoter-Driven Gene Expression in Primary CD4+ T Cells
    Article Snippet: Purified resting CD4+ T cells (1 × 106 /sample) isolated from HIV-1-infected individuals who had undergone combination antiretroviral therapy were coinfected with lentiviruses containing Naf1 shRNA or the scramble control (50 ng p24gag ) and lentiviruses containing Vpx (multiplicity of infection [MOI] = 0.5) for 6 h , and after washing, the cells were cultured for another 3 days. .. For RT-PCR, total cellular RNA was extracted by use of a QIAamp RNeasy minikit (Qiagen), and the HIV-1 gag or tat-rev mRNA was quantified and normalized to the GAPDH mRNA level.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Naf1 Regulates HIV-1 Latency by Suppressing Viral Promoter-Driven Gene Expression in Primary CD4+ T Cells
    Article Snippet: HIV-1 activation was detected by measuring the released HIV-1 particles in the supernatants by p24gag capture ELISA or by RT-PCR for quantification of the HIV-1 transcription products. .. For RT-PCR, total cellular RNA was extracted by use of a QIAamp RNeasy minikit (Qiagen), and the HIV-1 gag or tat-rev mRNA was quantified and normalized to the GAPDH mRNA level.

    Activation Assay:

    Article Title: Naf1 Regulates HIV-1 Latency by Suppressing Viral Promoter-Driven Gene Expression in Primary CD4+ T Cells
    Article Snippet: Paragraph title: Assays of viral activation in resting CD4+ T cells from HIV-1-infected individuals. ... For RT-PCR, total cellular RNA was extracted by use of a QIAamp RNeasy minikit (Qiagen), and the HIV-1 gag or tat-rev mRNA was quantified and normalized to the GAPDH mRNA level.

    CTG Assay:

    Article Title: Naf1 Regulates HIV-1 Latency by Suppressing Viral Promoter-Driven Gene Expression in Primary CD4+ T Cells
    Article Snippet: For RT-PCR, total cellular RNA was extracted by use of a QIAamp RNeasy minikit (Qiagen), and the HIV-1 gag or tat-rev mRNA was quantified and normalized to the GAPDH mRNA level. .. The primer sequences were as follows: for gag , forward primer 5′-GTG TGG AAA ATC TCT AGC AGT GG-3′ and reverse primer 5′-CGC TCT CGC ACC CAT CTC-3′; for tat-rev , forward primer 5′-ATG GCA GGA AGA AGC GGA G-3′ and reverse primer 5′-ATT CCT TCG GGC CTG TCG-3′; and for GAPDH, forward primer 5′-ATC CCA TCA CCA TCT TCC AGG-3′ and reverse primer 5′-CCT TCT CCA TGG TGG TGA AGA C-3′.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Qiagen qiaamp rneasy minikit
    Qiaamp Rneasy Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaamp rneasy minikit/product/Qiagen
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    qiaamp rneasy minikit - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    Image Search Results