qia quick qiagen pcr purification kit  (Qiagen)


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    QIAquick PCR Purification Kit
    Description:
    For purification of up to 10 μg PCR products 100 bp to 10 kb Kit contents Qiagen QIAquick PCR Purification Kit 50 rxns 30L Elution Volume 10g Binding Capacity Tube Format Manual Processing Silica Technology 100 bp to 10 kb Fragment 40mers Fragments Removed Ideal for Sequencing Microarray Analysis Ligation and Transformation Restriction Digestion Labeling Microinjection PCR and in vitro Transcription For Purification of up to 10μg PCR Products Includes 50 QIAquick Spin Columns Buffers 2mL Collection Tubes Benefits Up to 95 recovery of ready to use DNA Cleanup of DNA up to 10 kb in three easy steps Gel loading dye for convenient sample analysis
    Catalog Number:
    28104
    Price:
    117
    Category:
    QIAquick PCR Purification Kit
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    Structured Review

    Qiagen qia quick qiagen pcr purification kit
    QIAquick PCR Purification Kit
    For purification of up to 10 μg PCR products 100 bp to 10 kb Kit contents Qiagen QIAquick PCR Purification Kit 50 rxns 30L Elution Volume 10g Binding Capacity Tube Format Manual Processing Silica Technology 100 bp to 10 kb Fragment 40mers Fragments Removed Ideal for Sequencing Microarray Analysis Ligation and Transformation Restriction Digestion Labeling Microinjection PCR and in vitro Transcription For Purification of up to 10μg PCR Products Includes 50 QIAquick Spin Columns Buffers 2mL Collection Tubes Benefits Up to 95 recovery of ready to use DNA Cleanup of DNA up to 10 kb in three easy steps Gel loading dye for convenient sample analysis
    https://www.bioz.com/result/qia quick qiagen pcr purification kit/product/Qiagen
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qia quick qiagen pcr purification kit - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Arsenic(V) Reduction in Relation to Iron(III) Transformation and Molecular Characterization of the Structural and Functional Microbial Community in Sediments of a Basin-Fill Aquifer in Northern Utah
    Article Snippet: Paragraph title: Functional gene ( arrA ) cloning and DNA sequencing. ... Amplified products (equal concentrations of DNA) from the three replicates of each treatment were pooled, purified with the QIAquick PCR purification kit (Qiagen, Valencia, CA), ligated into the TOPO TA pCR4.0 vector, and transformed into Escherichia coli One Shot TOP10 competent cells (TOPO TA cloning kit; Life Technologies, CA) as recommended by the manufacturer.

    Article Title: Dihydropteroate Synthase of Mycobacterium leprae and Dapsone Resistance
    Article Snippet: Paragraph title: Cloning of folP homologs and complementation of folP knockout mutants. ... The resultant PCR fragments were purified and concentrated using a QIAQuick PCR Purification Kit (QIAGEN, Valencia, Calif.), and DNA sequences were obtained by automated sequencing on a PE BioSystems 377 automated DNA sequencer (Perkin-Elmer, Gaithersburg, Md.).

    Centrifugation:

    Article Title: Germ Line Variants of Human N-Methylpurine DNA Glycosylase Show Impaired DNA Repair Activity and Facilitate 1,N6-Ethenoadenine-induced Mutations *
    Article Snippet: Six microliters of purified oligonucleotide was incubated with 2 μg of pBS-SV40 ssDNA in annealing buffer (10 m m Tris-HCl, pH 7.5, 50 m m NaCl) at 80 °C for 5 min and then gradually cooled to room temperature with brief centrifugation at 50 °C. .. Finally, the reaction was incubated with 1× Supercoil-It buffer (Bayou Biolabs, Metairie, LA) and Supercoil-It enzyme at 37 °C for 3 h. Plasmid DNA was recovered using the QIAquick PCR Purification kit (Qiagen).

    Amplification:

    Article Title: Integron-Containing IncU R Plasmids pRAS1 and pAr-32 from the Fish Pathogen Aeromonas salmonicida
    Article Snippet: Paragraph title: PCR amplification and sequencing of amplified products. ... PCR amplicons were purified by use of the Qiaquick PCR purification kit (Qiagen GmbH), the reaction mixtures for the sequencing PCR were prepared, and the products were purified as recommended in the protocol of the BigDye Terminator Ready Reaction kit (Applied Biosystems, Warrington, United Kingdom).

    Article Title: Arsenic(V) Reduction in Relation to Iron(III) Transformation and Molecular Characterization of the Structural and Functional Microbial Community in Sediments of a Basin-Fill Aquifer in Northern Utah
    Article Snippet: .. Amplified products (equal concentrations of DNA) from the three replicates of each treatment were pooled, purified with the QIAquick PCR purification kit (Qiagen, Valencia, CA), ligated into the TOPO TA pCR4.0 vector, and transformed into Escherichia coli One Shot TOP10 competent cells (TOPO TA cloning kit; Life Technologies, CA) as recommended by the manufacturer. .. A total of six independent clone libraries were generated and a minimum of 45 arrA gene clones were sequenced per treatment (total of 288 sequences).

    Article Title: Essential role for polymerase specialization in cellular nonhomologous end joining
    Article Snippet: Substrates were prepared by PCR amplification of a common 285-bp DNA segment with primer pairs containing embedded restriction enzyme digest sites chosen to generate the desired end structures. .. Substrates were purified using the QIAquick PCR purification kit (Qiagen), and complete digestion of each substrate was validated by native gel electrophoresis.

    Article Title: Regime Shift and Microbial Dynamics in a Sequencing Batch Reactor for Nitrification and Anammox Treatment of Urine ▿Regime Shift and Microbial Dynamics in a Sequencing Batch Reactor for Nitrification and Anammox Treatment of Urine ▿ †
    Article Snippet: DGGE band sequencing (short sequence reads) and a nearly full-length amplicon clone library sequencing approach (long reads) were combined to obtain an overview of the microbial community compositions. .. Successful PCR products were purified using a QIAquick PCR purification kit (Qiagen), and commercial sequencing was performed by Microsynth (Balgach, Switzerland).

    Article Title: Sequence-Based Optimization of a Quantitative Real-Time PCR Assay for Detection of Plasmodium ovale and Plasmodium malariae
    Article Snippet: Paragraph title: Amplification and sequencing of the 18S rRNA region of P. malariae and P. ovale . ... PCR products were purified with a QIAquick PCR purification kit (Qiagen, Germantown, MD) and eluted with 20 μl water.

    Article Title: Use of Inverse PCR for Analysis of Class 1 Integrons Carrying an Unusual 3? Conserved Segment Structure ▿
    Article Snippet: For step 4 (inverse PCR amplification), inverse PCR amplification was performed using 1.25 units TaKaRa Ex Taq DNA polymerase (TaKaRa Bio Inc., Otsu, Shiga, Japan) for amplification of longer products. .. Finally, the inverse PCR products were purified using the QIAquick PCR purification kit (Qiagen Inc., Valencia, California) for primer-walking-strategy DNA sequencing and then analyzed using the BLAST suite of programs.

    Article Title: Dihydropteroate Synthase of Mycobacterium leprae and Dapsone Resistance
    Article Snippet: Clones containing M. leprae folP1 or folP2 were identified by PCR amplification of the respective gene from crude cell lysates of selected bacterial colonies using folP1-7 and -8 and folP2-1 and -2. .. The resultant PCR fragments were purified and concentrated using a QIAQuick PCR Purification Kit (QIAGEN, Valencia, Calif.), and DNA sequences were obtained by automated sequencing on a PE BioSystems 377 automated DNA sequencer (Perkin-Elmer, Gaithersburg, Md.).

    TA Cloning:

    Article Title: Arsenic(V) Reduction in Relation to Iron(III) Transformation and Molecular Characterization of the Structural and Functional Microbial Community in Sediments of a Basin-Fill Aquifer in Northern Utah
    Article Snippet: .. Amplified products (equal concentrations of DNA) from the three replicates of each treatment were pooled, purified with the QIAquick PCR purification kit (Qiagen, Valencia, CA), ligated into the TOPO TA pCR4.0 vector, and transformed into Escherichia coli One Shot TOP10 competent cells (TOPO TA cloning kit; Life Technologies, CA) as recommended by the manufacturer. .. A total of six independent clone libraries were generated and a minimum of 45 arrA gene clones were sequenced per treatment (total of 288 sequences).

    Construct:

    Article Title: Germ Line Variants of Human N-Methylpurine DNA Glycosylase Show Impaired DNA Repair Activity and Facilitate 1,N6-Ethenoadenine-induced Mutations *
    Article Snippet: Paragraph title: ϵA or 8-Oxo-dG pBS-SV40 in Vitro Construct Preparation ... Finally, the reaction was incubated with 1× Supercoil-It buffer (Bayou Biolabs, Metairie, LA) and Supercoil-It enzyme at 37 °C for 3 h. Plasmid DNA was recovered using the QIAquick PCR Purification kit (Qiagen).

    SYBR Green Assay:

    Article Title: Analysis of chromatin binding dynamics using the crosslinking kinetics (CLK) method
    Article Snippet: The following day, all samples are cleaned up with the QIAquick PCR purification kit (Qiagen) according to the manufacturer’s instructions and the purified DNA is eluted from the columns with 50 µl of 50°C DEPC-treated water. .. We employ a Bio-Rad MyiQ instrument and use Bio-Rad iQ SYBR Green Supermix in combination with forward and reverse primer concentrations of 5 µM and 1 µL template.

    Article Title: Minimally invasive sampling method identifies differences in taxonomic richness of nasal microbiomes in young infants associated with mode of delivery
    Article Snippet: Amplicons were cleaned using the QIAquick PCR Purification Kit (QIAGEN, Valencia, CA), as per the manufacturer’s instructions, with an additional drying step after the ethanol wash to ensure complete ethanol removal. .. Purified amplicons were quantified using SYBR Green (Life Technologies, Grand Island, NY) on a Synergy HT plate reader (BioTek, Winooski, VT), normalized, and pooled.

    Nested PCR:

    Article Title: Arsenic(V) Reduction in Relation to Iron(III) Transformation and Molecular Characterization of the Structural and Functional Microbial Community in Sediments of a Basin-Fill Aquifer in Northern Utah
    Article Snippet: The arsenate reductase ( arrA ) gene was amplified from the genomic DNA using a nested PCR approach as described previously ( ). .. Amplified products (equal concentrations of DNA) from the three replicates of each treatment were pooled, purified with the QIAquick PCR purification kit (Qiagen, Valencia, CA), ligated into the TOPO TA pCR4.0 vector, and transformed into Escherichia coli One Shot TOP10 competent cells (TOPO TA cloning kit; Life Technologies, CA) as recommended by the manufacturer.

    Incubation:

    Article Title: Germ Line Variants of Human N-Methylpurine DNA Glycosylase Show Impaired DNA Repair Activity and Facilitate 1,N6-Ethenoadenine-induced Mutations *
    Article Snippet: .. Finally, the reaction was incubated with 1× Supercoil-It buffer (Bayou Biolabs, Metairie, LA) and Supercoil-It enzyme at 37 °C for 3 h. Plasmid DNA was recovered using the QIAquick PCR Purification kit (Qiagen). .. The damaged (ϵA and 8-oxo-dG) constructs were transfected into cells with Lipofectamine 2000 (Invitrogen) following the manufacturer's protocol.

    Article Title: TFIIH Subunit Alterations Causing Xeroderma Pigmentosum and Trichothiodystrophy Specifically Disturb Several Steps during Transcription
    Article Snippet: Samples were immunoprecipitated with antibodies at 4°C overnight, and protein G Sepharose beads (Upstate) were added, incubated for 4 hr at 4°C, and sequentially washed. .. DNA fragments were purified with the QIAquick PCR Purification Kit (QIAGEN) and analyzed by qPCR with a set of primers targeting the promoter and terminator regions of RARB2 ( ).

    Article Title: A cell-cell signaling peptide activates the PlcR virulence regulon in bacteria of the Bacillus cereus group
    Article Snippet: Labeled PCR products were purified with the Qiaquick PCR purification kit (Qiagen). .. After incubation for 1 min at room temperature, the reaction was stopped by adding 200 µl of stop buffer (0.4 M sodium acetate, 50 µg/ml sonicated calf thymus DNA, 2.5 mM EDTA).

    Expressing:

    Article Title: Immune Failure in the Absence of Profound CD4+ T-Lymphocyte Depletion in Simian Immunodeficiency Virus-Infected Rapid Progressor Macaques
    Article Snippet: Verification was achieved by direct sequencing of PCR products (QIAquick PCR purification kit, Qiagen) by automated sequencing on the ABI 377 sequencer. .. The ability of herpesvirus papio-transformed B lymphoblastoid cell lines (B-LCL) from these macaques to act as targets in p11C-specific functional cytotoxicity assays was used to confirm the expression of the Mamu-A*01 allele.

    Article Title: Attenuation of lung cancer stem cell tumorigenesis and metastasis by cisplatin
    Article Snippet: Paragraph title: Digital Gene Expression Sequencing of RNA and Statistical Analysis ... The RNA was first fragmented into small pieces and then cDNA libraries were prepared according to the manufacturer's instruction (Illumina Inc., USA) and purified by the QIAquick PCR Purification Kit (Qiagen).

    Transformation Assay:

    Article Title: Arsenic(V) Reduction in Relation to Iron(III) Transformation and Molecular Characterization of the Structural and Functional Microbial Community in Sediments of a Basin-Fill Aquifer in Northern Utah
    Article Snippet: .. Amplified products (equal concentrations of DNA) from the three replicates of each treatment were pooled, purified with the QIAquick PCR purification kit (Qiagen, Valencia, CA), ligated into the TOPO TA pCR4.0 vector, and transformed into Escherichia coli One Shot TOP10 competent cells (TOPO TA cloning kit; Life Technologies, CA) as recommended by the manufacturer. .. A total of six independent clone libraries were generated and a minimum of 45 arrA gene clones were sequenced per treatment (total of 288 sequences).

    Article Title: Dihydropteroate Synthase of Mycobacterium leprae and Dapsone Resistance
    Article Snippet: E. coli XL-1 Blue cells were transformed with these plasmids, and recombinant clones were selected on LB agar containing 100 μg of ampicillin/ml. .. The resultant PCR fragments were purified and concentrated using a QIAQuick PCR Purification Kit (QIAGEN, Valencia, Calif.), and DNA sequences were obtained by automated sequencing on a PE BioSystems 377 automated DNA sequencer (Perkin-Elmer, Gaithersburg, Md.).

    Hybridization:

    Article Title: Use of Inverse PCR for Analysis of Class 1 Integrons Carrying an Unusual 3? Conserved Segment Structure ▿
    Article Snippet: Finally, the inverse PCR products were purified using the QIAquick PCR purification kit (Qiagen Inc., Valencia, California) for primer-walking-strategy DNA sequencing and then analyzed using the BLAST suite of programs. .. In addition, conjugation experiments and Southern hybridization were carried out to demonstrate genetic localization of class 1 integron with unusual 3′ ends.

    Conjugation Assay:

    Article Title: Use of Inverse PCR for Analysis of Class 1 Integrons Carrying an Unusual 3? Conserved Segment Structure ▿
    Article Snippet: Finally, the inverse PCR products were purified using the QIAquick PCR purification kit (Qiagen Inc., Valencia, California) for primer-walking-strategy DNA sequencing and then analyzed using the BLAST suite of programs. .. In addition, conjugation experiments and Southern hybridization were carried out to demonstrate genetic localization of class 1 integron with unusual 3′ ends.

    Flow Cytometry:

    Article Title: Immune Failure in the Absence of Profound CD4+ T-Lymphocyte Depletion in Simian Immunodeficiency Virus-Infected Rapid Progressor Macaques
    Article Snippet: EDTA-anticoagulated blood samples were collected sequentially and evaluated for isolation of infectious SIV, plasma viral RNA loads, lymphocyte subsets by flow cytometry, SIV-specific antibody, and cytotoxic T-cell responses. .. Verification was achieved by direct sequencing of PCR products (QIAquick PCR purification kit, Qiagen) by automated sequencing on the ABI 377 sequencer.

    Inverse PCR:

    Article Title: Use of Inverse PCR for Analysis of Class 1 Integrons Carrying an Unusual 3? Conserved Segment Structure ▿
    Article Snippet: .. Finally, the inverse PCR products were purified using the QIAquick PCR purification kit (Qiagen Inc., Valencia, California) for primer-walking-strategy DNA sequencing and then analyzed using the BLAST suite of programs. .. In addition, conjugation experiments and Southern hybridization were carried out to demonstrate genetic localization of class 1 integron with unusual 3′ ends.

    Ligation:

    Article Title: Germ Line Variants of Human N-Methylpurine DNA Glycosylase Show Impaired DNA Repair Activity and Facilitate 1,N6-Ethenoadenine-induced Mutations *
    Article Snippet: The reaction was subsequently incubated at 37 °C for 1 h after which 50 nmol of ATP and 200 units of T4 DNA ligase were added and incubated at 14 °C overnight for efficient ligation. .. Finally, the reaction was incubated with 1× Supercoil-It buffer (Bayou Biolabs, Metairie, LA) and Supercoil-It enzyme at 37 °C for 3 h. Plasmid DNA was recovered using the QIAquick PCR Purification kit (Qiagen).

    Article Title: Use of Inverse PCR for Analysis of Class 1 Integrons Carrying an Unusual 3? Conserved Segment Structure ▿
    Article Snippet: The intramolecular ligation products were then used as substrates for PCR analysis using specific primers (Table ) for amplification of regions that flank the known sequence. .. Finally, the inverse PCR products were purified using the QIAquick PCR purification kit (Qiagen Inc., Valencia, California) for primer-walking-strategy DNA sequencing and then analyzed using the BLAST suite of programs.

    Footprinting:

    Article Title: A cell-cell signaling peptide activates the PlcR virulence regulon in bacteria of the Bacillus cereus group
    Article Snippet: Paragraph title: DNase I footprinting ... Labeled PCR products were purified with the Qiaquick PCR purification kit (Qiagen).

    Infection:

    Article Title: Immune Failure in the Absence of Profound CD4+ T-Lymphocyte Depletion in Simian Immunodeficiency Virus-Infected Rapid Progressor Macaques
    Article Snippet: The two rhesus macaques infected with SIVsmE660 expressed the Mamu-A*01 MHC class I molecule. .. Verification was achieved by direct sequencing of PCR products (QIAquick PCR purification kit, Qiagen) by automated sequencing on the ABI 377 sequencer.

    Generated:

    Article Title: Arsenic(V) Reduction in Relation to Iron(III) Transformation and Molecular Characterization of the Structural and Functional Microbial Community in Sediments of a Basin-Fill Aquifer in Northern Utah
    Article Snippet: Amplified products (equal concentrations of DNA) from the three replicates of each treatment were pooled, purified with the QIAquick PCR purification kit (Qiagen, Valencia, CA), ligated into the TOPO TA pCR4.0 vector, and transformed into Escherichia coli One Shot TOP10 competent cells (TOPO TA cloning kit; Life Technologies, CA) as recommended by the manufacturer. .. A total of six independent clone libraries were generated and a minimum of 45 arrA gene clones were sequenced per treatment (total of 288 sequences).

    Article Title: Minimally invasive sampling method identifies differences in taxonomic richness of nasal microbiomes in young infants associated with mode of delivery
    Article Snippet: Amplicons were generated with Platinum Taq polymerase (Life Technologies, Grand Island, NY) using the following cycling conditions: 95 °C for 5 minutes; 35 cycles of 95 °C for 30 seconds, 55 °C for 30 seconds, 72 °C for 30 seconds; and a final extension step at 72 °C for 7 minutes. .. Amplicons were cleaned using the QIAquick PCR Purification Kit (QIAGEN, Valencia, CA), as per the manufacturer’s instructions, with an additional drying step after the ethanol wash to ensure complete ethanol removal.

    DNA Sequencing:

    Article Title: Arsenic(V) Reduction in Relation to Iron(III) Transformation and Molecular Characterization of the Structural and Functional Microbial Community in Sediments of a Basin-Fill Aquifer in Northern Utah
    Article Snippet: Paragraph title: Functional gene ( arrA ) cloning and DNA sequencing. ... Amplified products (equal concentrations of DNA) from the three replicates of each treatment were pooled, purified with the QIAquick PCR purification kit (Qiagen, Valencia, CA), ligated into the TOPO TA pCR4.0 vector, and transformed into Escherichia coli One Shot TOP10 competent cells (TOPO TA cloning kit; Life Technologies, CA) as recommended by the manufacturer.

    Article Title: Use of Inverse PCR for Analysis of Class 1 Integrons Carrying an Unusual 3? Conserved Segment Structure ▿
    Article Snippet: .. Finally, the inverse PCR products were purified using the QIAquick PCR purification kit (Qiagen Inc., Valencia, California) for primer-walking-strategy DNA sequencing and then analyzed using the BLAST suite of programs. .. In addition, conjugation experiments and Southern hybridization were carried out to demonstrate genetic localization of class 1 integron with unusual 3′ ends.

    Article Title: Dihydropteroate Synthase of Mycobacterium leprae and Dapsone Resistance
    Article Snippet: The resultant PCR fragments were purified and concentrated using a QIAQuick PCR Purification Kit (QIAGEN, Valencia, Calif.), and DNA sequences were obtained by automated sequencing on a PE BioSystems 377 automated DNA sequencer (Perkin-Elmer, Gaithersburg, Md.). .. The presence of M. leprae folP1 or folP2 in recombinant clones was confirmed by PCR and DNA sequencing as described above.

    Sequencing:

    Article Title: Integron-Containing IncU R Plasmids pRAS1 and pAr-32 from the Fish Pathogen Aeromonas salmonicida
    Article Snippet: .. PCR amplicons were purified by use of the Qiaquick PCR purification kit (Qiagen GmbH), the reaction mixtures for the sequencing PCR were prepared, and the products were purified as recommended in the protocol of the BigDye Terminator Ready Reaction kit (Applied Biosystems, Warrington, United Kingdom). .. The products from the sequencing PCR were separated with an ABI Prism 377 automated sequencing machine (Perkin-Elmer Cetus Corp., Foster City, Calif.), and the DNA sequences were analyzed by using the GCG Sequence Analysis software package (version 8; Genetics Computer Group, Madison, Wis.) and BLAST search analysis ( ).

    Article Title: Arsenic(V) Reduction in Relation to Iron(III) Transformation and Molecular Characterization of the Structural and Functional Microbial Community in Sediments of a Basin-Fill Aquifer in Northern Utah
    Article Snippet: Amplified products (equal concentrations of DNA) from the three replicates of each treatment were pooled, purified with the QIAquick PCR purification kit (Qiagen, Valencia, CA), ligated into the TOPO TA pCR4.0 vector, and transformed into Escherichia coli One Shot TOP10 competent cells (TOPO TA cloning kit; Life Technologies, CA) as recommended by the manufacturer. .. Sequencing was performed at the DNA Analysis Facility on Science Hill at Yale University.

    Article Title: Immune Failure in the Absence of Profound CD4+ T-Lymphocyte Depletion in Simian Immunodeficiency Virus-Infected Rapid Progressor Macaques
    Article Snippet: .. Verification was achieved by direct sequencing of PCR products (QIAquick PCR purification kit, Qiagen) by automated sequencing on the ABI 377 sequencer. .. The ability of herpesvirus papio-transformed B lymphoblastoid cell lines (B-LCL) from these macaques to act as targets in p11C-specific functional cytotoxicity assays was used to confirm the expression of the Mamu-A*01 allele.

    Article Title: Attenuation of lung cancer stem cell tumorigenesis and metastasis by cisplatin
    Article Snippet: Paragraph title: Digital Gene Expression Sequencing of RNA and Statistical Analysis ... The RNA was first fragmented into small pieces and then cDNA libraries were prepared according to the manufacturer's instruction (Illumina Inc., USA) and purified by the QIAquick PCR Purification Kit (Qiagen).

    Article Title: Regime Shift and Microbial Dynamics in a Sequencing Batch Reactor for Nitrification and Anammox Treatment of Urine ▿Regime Shift and Microbial Dynamics in a Sequencing Batch Reactor for Nitrification and Anammox Treatment of Urine ▿ †
    Article Snippet: .. Successful PCR products were purified using a QIAquick PCR purification kit (Qiagen), and commercial sequencing was performed by Microsynth (Balgach, Switzerland). ..

    Article Title: Sequence-Based Optimization of a Quantitative Real-Time PCR Assay for Detection of Plasmodium ovale and Plasmodium malariae
    Article Snippet: Paragraph title: Amplification and sequencing of the 18S rRNA region of P. malariae and P. ovale . ... PCR products were purified with a QIAquick PCR purification kit (Qiagen, Germantown, MD) and eluted with 20 μl water.

    Article Title: Use of Inverse PCR for Analysis of Class 1 Integrons Carrying an Unusual 3? Conserved Segment Structure ▿
    Article Snippet: The intramolecular ligation products were then used as substrates for PCR analysis using specific primers (Table ) for amplification of regions that flank the known sequence. .. Finally, the inverse PCR products were purified using the QIAquick PCR purification kit (Qiagen Inc., Valencia, California) for primer-walking-strategy DNA sequencing and then analyzed using the BLAST suite of programs.

    Sonication:

    Article Title: TFIIH Subunit Alterations Causing Xeroderma Pigmentosum and Trichothiodystrophy Specifically Disturb Several Steps during Transcription
    Article Snippet: Chromatin was prepared and sonicated on ice for 30 min with a Bioruptor (Diagenode) as previously described. .. DNA fragments were purified with the QIAquick PCR Purification Kit (QIAGEN) and analyzed by qPCR with a set of primers targeting the promoter and terminator regions of RARB2 ( ).

    Article Title: A cell-cell signaling peptide activates the PlcR virulence regulon in bacteria of the Bacillus cereus group
    Article Snippet: Labeled PCR products were purified with the Qiaquick PCR purification kit (Qiagen). .. After incubation for 1 min at room temperature, the reaction was stopped by adding 200 µl of stop buffer (0.4 M sodium acetate, 50 µg/ml sonicated calf thymus DNA, 2.5 mM EDTA).

    Binding Assay:

    Article Title: A cell-cell signaling peptide activates the PlcR virulence regulon in bacteria of the Bacillus cereus group
    Article Snippet: Labeled PCR products were purified with the Qiaquick PCR purification kit (Qiagen). .. Assays of PlcR binding to DNA were performed as follows, with the addition of BSA (1 µg).

    Nucleic Acid Electrophoresis:

    Article Title: Essential role for polymerase specialization in cellular nonhomologous end joining
    Article Snippet: .. Substrates were purified using the QIAquick PCR purification kit (Qiagen), and complete digestion of each substrate was validated by native gel electrophoresis. ..

    Article Title: Regime Shift and Microbial Dynamics in a Sequencing Batch Reactor for Nitrification and Anammox Treatment of Urine ▿Regime Shift and Microbial Dynamics in a Sequencing Batch Reactor for Nitrification and Anammox Treatment of Urine ▿ †
    Article Snippet: DGGE was performed using general bacterial primers 341F-GC and 534R as described in reference using a DCode DGGE system (Bio-Rad Laboratories AG, Reinach, Switzerland) for gel electrophoresis. .. Successful PCR products were purified using a QIAquick PCR purification kit (Qiagen), and commercial sequencing was performed by Microsynth (Balgach, Switzerland).

    RNA Sequencing Assay:

    Article Title: Attenuation of lung cancer stem cell tumorigenesis and metastasis by cisplatin
    Article Snippet: A total of 20 μg RNA of each sample was used for RNA sequencing. .. The RNA was first fragmented into small pieces and then cDNA libraries were prepared according to the manufacturer's instruction (Illumina Inc., USA) and purified by the QIAquick PCR Purification Kit (Qiagen).

    Isolation:

    Article Title: Integron-Containing IncU R Plasmids pRAS1 and pAr-32 from the Fish Pathogen Aeromonas salmonicida
    Article Snippet: Template DNA either was isolated plasmid DNA or was from boiled bacterial suspensions. .. PCR amplicons were purified by use of the Qiaquick PCR purification kit (Qiagen GmbH), the reaction mixtures for the sequencing PCR were prepared, and the products were purified as recommended in the protocol of the BigDye Terminator Ready Reaction kit (Applied Biosystems, Warrington, United Kingdom).

    Article Title: Immune Failure in the Absence of Profound CD4+ T-Lymphocyte Depletion in Simian Immunodeficiency Virus-Infected Rapid Progressor Macaques
    Article Snippet: These animals were selected by Mamu-A*01-specific reverse transcriptase-PCR of total RNA isolated by RNeasy purification (Qiagen, Chatsworth, Calif.) from 5 × 106 peripheral blood mononuclear cells as described previously. .. Verification was achieved by direct sequencing of PCR products (QIAquick PCR purification kit, Qiagen) by automated sequencing on the ABI 377 sequencer.

    Article Title: Attenuation of lung cancer stem cell tumorigenesis and metastasis by cisplatin
    Article Snippet: Total RNA of the −Cis and +Cis tumors was isolated using Trizol (Invitrogen, USA) and measured using Agilent 2100 Bioanalyzer (Agilent Technologies, USA). .. The RNA was first fragmented into small pieces and then cDNA libraries were prepared according to the manufacturer's instruction (Illumina Inc., USA) and purified by the QIAquick PCR Purification Kit (Qiagen).

    Article Title: Use of Inverse PCR for Analysis of Class 1 Integrons Carrying an Unusual 3? Conserved Segment Structure ▿
    Article Snippet: Finally, the inverse PCR products were purified using the QIAquick PCR purification kit (Qiagen Inc., Valencia, California) for primer-walking-strategy DNA sequencing and then analyzed using the BLAST suite of programs. .. Plasmid DNA was isolated using an alkaline lysis method ( ).

    Transfection:

    Article Title: West Nile virus (WNV) genome RNAs with up to three adjacent mutations that disrupt long distance 5?-3? cyclization sequence basepairs are viable
    Article Snippet: Paragraph title: Transfection of in vitro transcribed viral RNA and assay of progeny virus replication ... Parental or mutated infectious clone DNA was linearized by digestion with XbaI and then purified using a Qiaquick PCR Purification kit (Qiagen).

    Labeling:

    Article Title: A cell-cell signaling peptide activates the PlcR virulence regulon in bacteria of the Bacillus cereus group
    Article Snippet: .. Labeled PCR products were purified with the Qiaquick PCR purification kit (Qiagen). .. Assays of PlcR binding to DNA were performed as follows, with the addition of BSA (1 µg).

    Purification:

    Article Title: Integron-Containing IncU R Plasmids pRAS1 and pAr-32 from the Fish Pathogen Aeromonas salmonicida
    Article Snippet: .. PCR amplicons were purified by use of the Qiaquick PCR purification kit (Qiagen GmbH), the reaction mixtures for the sequencing PCR were prepared, and the products were purified as recommended in the protocol of the BigDye Terminator Ready Reaction kit (Applied Biosystems, Warrington, United Kingdom). .. The products from the sequencing PCR were separated with an ABI Prism 377 automated sequencing machine (Perkin-Elmer Cetus Corp., Foster City, Calif.), and the DNA sequences were analyzed by using the GCG Sequence Analysis software package (version 8; Genetics Computer Group, Madison, Wis.) and BLAST search analysis ( ).

    Article Title: Arsenic(V) Reduction in Relation to Iron(III) Transformation and Molecular Characterization of the Structural and Functional Microbial Community in Sediments of a Basin-Fill Aquifer in Northern Utah
    Article Snippet: .. Amplified products (equal concentrations of DNA) from the three replicates of each treatment were pooled, purified with the QIAquick PCR purification kit (Qiagen, Valencia, CA), ligated into the TOPO TA pCR4.0 vector, and transformed into Escherichia coli One Shot TOP10 competent cells (TOPO TA cloning kit; Life Technologies, CA) as recommended by the manufacturer. .. A total of six independent clone libraries were generated and a minimum of 45 arrA gene clones were sequenced per treatment (total of 288 sequences).

    Article Title: West Nile virus (WNV) genome RNAs with up to three adjacent mutations that disrupt long distance 5?-3? cyclization sequence basepairs are viable
    Article Snippet: .. Parental or mutated infectious clone DNA was linearized by digestion with XbaI and then purified using a Qiaquick PCR Purification kit (Qiagen). .. Capped viral RNA was transcribed in vitro using an SP6 mMessage mMachine High Yield Capped RNA Transcription kit (Ambion) according to the manufacturer's protocol.

    Article Title: Immune Failure in the Absence of Profound CD4+ T-Lymphocyte Depletion in Simian Immunodeficiency Virus-Infected Rapid Progressor Macaques
    Article Snippet: .. Verification was achieved by direct sequencing of PCR products (QIAquick PCR purification kit, Qiagen) by automated sequencing on the ABI 377 sequencer. .. The ability of herpesvirus papio-transformed B lymphoblastoid cell lines (B-LCL) from these macaques to act as targets in p11C-specific functional cytotoxicity assays was used to confirm the expression of the Mamu-A*01 allele.

    Article Title: Essential role for polymerase specialization in cellular nonhomologous end joining
    Article Snippet: .. Substrates were purified using the QIAquick PCR purification kit (Qiagen), and complete digestion of each substrate was validated by native gel electrophoresis. ..

    Article Title: Attenuation of lung cancer stem cell tumorigenesis and metastasis by cisplatin
    Article Snippet: .. The RNA was first fragmented into small pieces and then cDNA libraries were prepared according to the manufacturer's instruction (Illumina Inc., USA) and purified by the QIAquick PCR Purification Kit (Qiagen). ..

    Article Title: Regime Shift and Microbial Dynamics in a Sequencing Batch Reactor for Nitrification and Anammox Treatment of Urine ▿Regime Shift and Microbial Dynamics in a Sequencing Batch Reactor for Nitrification and Anammox Treatment of Urine ▿ †
    Article Snippet: .. Successful PCR products were purified using a QIAquick PCR purification kit (Qiagen), and commercial sequencing was performed by Microsynth (Balgach, Switzerland). ..

    Article Title: Germ Line Variants of Human N-Methylpurine DNA Glycosylase Show Impaired DNA Repair Activity and Facilitate 1,N6-Ethenoadenine-induced Mutations *
    Article Snippet: .. Finally, the reaction was incubated with 1× Supercoil-It buffer (Bayou Biolabs, Metairie, LA) and Supercoil-It enzyme at 37 °C for 3 h. Plasmid DNA was recovered using the QIAquick PCR Purification kit (Qiagen). .. The damaged (ϵA and 8-oxo-dG) constructs were transfected into cells with Lipofectamine 2000 (Invitrogen) following the manufacturer's protocol.

    Article Title: TFIIH Subunit Alterations Causing Xeroderma Pigmentosum and Trichothiodystrophy Specifically Disturb Several Steps during Transcription
    Article Snippet: .. DNA fragments were purified with the QIAquick PCR Purification Kit (QIAGEN) and analyzed by qPCR with a set of primers targeting the promoter and terminator regions of RARB2 ( ). .. In parallel, cross-linked cells were permeabilized with cytonin (Trevigen) for 30 min at RT.

    Article Title: Sequence-Based Optimization of a Quantitative Real-Time PCR Assay for Detection of Plasmodium ovale and Plasmodium malariae
    Article Snippet: .. PCR products were purified with a QIAquick PCR purification kit (Qiagen, Germantown, MD) and eluted with 20 μl water. .. Purified PCR products were Sanger sequenced using the same forward and reverse primers with a BigDye Terminator v3.1 cycle sequencing kit (Life Technologies) and run according to the manufacturer's recommended conditions.

    Article Title: Use of Inverse PCR for Analysis of Class 1 Integrons Carrying an Unusual 3? Conserved Segment Structure ▿
    Article Snippet: .. Finally, the inverse PCR products were purified using the QIAquick PCR purification kit (Qiagen Inc., Valencia, California) for primer-walking-strategy DNA sequencing and then analyzed using the BLAST suite of programs. .. In addition, conjugation experiments and Southern hybridization were carried out to demonstrate genetic localization of class 1 integron with unusual 3′ ends.

    Article Title: Analysis of chromatin binding dynamics using the crosslinking kinetics (CLK) method
    Article Snippet: .. The following day, all samples are cleaned up with the QIAquick PCR purification kit (Qiagen) according to the manufacturer’s instructions and the purified DNA is eluted from the columns with 50 µl of 50°C DEPC-treated water. .. ChIP DNA is then quantified by RT-qPCR with input chromatin used for the standard curve.

    Article Title: Minimally invasive sampling method identifies differences in taxonomic richness of nasal microbiomes in young infants associated with mode of delivery
    Article Snippet: .. Amplicons were cleaned using the QIAquick PCR Purification Kit (QIAGEN, Valencia, CA), as per the manufacturer’s instructions, with an additional drying step after the ethanol wash to ensure complete ethanol removal. .. Purified amplicons were quantified using SYBR Green (Life Technologies, Grand Island, NY) on a Synergy HT plate reader (BioTek, Winooski, VT), normalized, and pooled.

    Article Title: A cell-cell signaling peptide activates the PlcR virulence regulon in bacteria of the Bacillus cereus group
    Article Snippet: .. Labeled PCR products were purified with the Qiaquick PCR purification kit (Qiagen). .. Assays of PlcR binding to DNA were performed as follows, with the addition of BSA (1 µg).

    Cytometry:

    Article Title: Immune Failure in the Absence of Profound CD4+ T-Lymphocyte Depletion in Simian Immunodeficiency Virus-Infected Rapid Progressor Macaques
    Article Snippet: EDTA-anticoagulated blood samples were collected sequentially and evaluated for isolation of infectious SIV, plasma viral RNA loads, lymphocyte subsets by flow cytometry, SIV-specific antibody, and cytotoxic T-cell responses. .. Verification was achieved by direct sequencing of PCR products (QIAquick PCR purification kit, Qiagen) by automated sequencing on the ABI 377 sequencer.

    Polymerase Chain Reaction:

    Article Title: Integron-Containing IncU R Plasmids pRAS1 and pAr-32 from the Fish Pathogen Aeromonas salmonicida
    Article Snippet: .. PCR amplicons were purified by use of the Qiaquick PCR purification kit (Qiagen GmbH), the reaction mixtures for the sequencing PCR were prepared, and the products were purified as recommended in the protocol of the BigDye Terminator Ready Reaction kit (Applied Biosystems, Warrington, United Kingdom). .. The products from the sequencing PCR were separated with an ABI Prism 377 automated sequencing machine (Perkin-Elmer Cetus Corp., Foster City, Calif.), and the DNA sequences were analyzed by using the GCG Sequence Analysis software package (version 8; Genetics Computer Group, Madison, Wis.) and BLAST search analysis ( ).

    Article Title: Arsenic(V) Reduction in Relation to Iron(III) Transformation and Molecular Characterization of the Structural and Functional Microbial Community in Sediments of a Basin-Fill Aquifer in Northern Utah
    Article Snippet: .. Amplified products (equal concentrations of DNA) from the three replicates of each treatment were pooled, purified with the QIAquick PCR purification kit (Qiagen, Valencia, CA), ligated into the TOPO TA pCR4.0 vector, and transformed into Escherichia coli One Shot TOP10 competent cells (TOPO TA cloning kit; Life Technologies, CA) as recommended by the manufacturer. .. A total of six independent clone libraries were generated and a minimum of 45 arrA gene clones were sequenced per treatment (total of 288 sequences).

    Article Title: West Nile virus (WNV) genome RNAs with up to three adjacent mutations that disrupt long distance 5?-3? cyclization sequence basepairs are viable
    Article Snippet: .. Parental or mutated infectious clone DNA was linearized by digestion with XbaI and then purified using a Qiaquick PCR Purification kit (Qiagen). .. Capped viral RNA was transcribed in vitro using an SP6 mMessage mMachine High Yield Capped RNA Transcription kit (Ambion) according to the manufacturer's protocol.

    Article Title: Immune Failure in the Absence of Profound CD4+ T-Lymphocyte Depletion in Simian Immunodeficiency Virus-Infected Rapid Progressor Macaques
    Article Snippet: .. Verification was achieved by direct sequencing of PCR products (QIAquick PCR purification kit, Qiagen) by automated sequencing on the ABI 377 sequencer. .. The ability of herpesvirus papio-transformed B lymphoblastoid cell lines (B-LCL) from these macaques to act as targets in p11C-specific functional cytotoxicity assays was used to confirm the expression of the Mamu-A*01 allele.

    Article Title: Essential role for polymerase specialization in cellular nonhomologous end joining
    Article Snippet: .. Substrates were purified using the QIAquick PCR purification kit (Qiagen), and complete digestion of each substrate was validated by native gel electrophoresis. ..

    Article Title: Attenuation of lung cancer stem cell tumorigenesis and metastasis by cisplatin
    Article Snippet: .. The RNA was first fragmented into small pieces and then cDNA libraries were prepared according to the manufacturer's instruction (Illumina Inc., USA) and purified by the QIAquick PCR Purification Kit (Qiagen). ..

    Article Title: Regime Shift and Microbial Dynamics in a Sequencing Batch Reactor for Nitrification and Anammox Treatment of Urine ▿Regime Shift and Microbial Dynamics in a Sequencing Batch Reactor for Nitrification and Anammox Treatment of Urine ▿ †
    Article Snippet: .. Successful PCR products were purified using a QIAquick PCR purification kit (Qiagen), and commercial sequencing was performed by Microsynth (Balgach, Switzerland). ..

    Article Title: Germ Line Variants of Human N-Methylpurine DNA Glycosylase Show Impaired DNA Repair Activity and Facilitate 1,N6-Ethenoadenine-induced Mutations *
    Article Snippet: .. Finally, the reaction was incubated with 1× Supercoil-It buffer (Bayou Biolabs, Metairie, LA) and Supercoil-It enzyme at 37 °C for 3 h. Plasmid DNA was recovered using the QIAquick PCR Purification kit (Qiagen). .. The damaged (ϵA and 8-oxo-dG) constructs were transfected into cells with Lipofectamine 2000 (Invitrogen) following the manufacturer's protocol.

    Article Title: TFIIH Subunit Alterations Causing Xeroderma Pigmentosum and Trichothiodystrophy Specifically Disturb Several Steps during Transcription
    Article Snippet: .. DNA fragments were purified with the QIAquick PCR Purification Kit (QIAGEN) and analyzed by qPCR with a set of primers targeting the promoter and terminator regions of RARB2 ( ). .. In parallel, cross-linked cells were permeabilized with cytonin (Trevigen) for 30 min at RT.

    Article Title: Sequence-Based Optimization of a Quantitative Real-Time PCR Assay for Detection of Plasmodium ovale and Plasmodium malariae
    Article Snippet: .. PCR products were purified with a QIAquick PCR purification kit (Qiagen, Germantown, MD) and eluted with 20 μl water. .. Purified PCR products were Sanger sequenced using the same forward and reverse primers with a BigDye Terminator v3.1 cycle sequencing kit (Life Technologies) and run according to the manufacturer's recommended conditions.

    Article Title: Use of Inverse PCR for Analysis of Class 1 Integrons Carrying an Unusual 3? Conserved Segment Structure ▿
    Article Snippet: .. Finally, the inverse PCR products were purified using the QIAquick PCR purification kit (Qiagen Inc., Valencia, California) for primer-walking-strategy DNA sequencing and then analyzed using the BLAST suite of programs. .. In addition, conjugation experiments and Southern hybridization were carried out to demonstrate genetic localization of class 1 integron with unusual 3′ ends.

    Article Title: Analysis of chromatin binding dynamics using the crosslinking kinetics (CLK) method
    Article Snippet: .. The following day, all samples are cleaned up with the QIAquick PCR purification kit (Qiagen) according to the manufacturer’s instructions and the purified DNA is eluted from the columns with 50 µl of 50°C DEPC-treated water. .. ChIP DNA is then quantified by RT-qPCR with input chromatin used for the standard curve.

    Article Title: Minimally invasive sampling method identifies differences in taxonomic richness of nasal microbiomes in young infants associated with mode of delivery
    Article Snippet: .. Amplicons were cleaned using the QIAquick PCR Purification Kit (QIAGEN, Valencia, CA), as per the manufacturer’s instructions, with an additional drying step after the ethanol wash to ensure complete ethanol removal. .. Purified amplicons were quantified using SYBR Green (Life Technologies, Grand Island, NY) on a Synergy HT plate reader (BioTek, Winooski, VT), normalized, and pooled.

    Article Title: A cell-cell signaling peptide activates the PlcR virulence regulon in bacteria of the Bacillus cereus group
    Article Snippet: .. Labeled PCR products were purified with the Qiaquick PCR purification kit (Qiagen). .. Assays of PlcR binding to DNA were performed as follows, with the addition of BSA (1 µg).

    Quantitative RT-PCR:

    Article Title: Analysis of chromatin binding dynamics using the crosslinking kinetics (CLK) method
    Article Snippet: The following day, all samples are cleaned up with the QIAquick PCR purification kit (Qiagen) according to the manufacturer’s instructions and the purified DNA is eluted from the columns with 50 µl of 50°C DEPC-treated water. .. ChIP DNA is then quantified by RT-qPCR with input chromatin used for the standard curve.

    cDNA Library Assay:

    Article Title: Attenuation of lung cancer stem cell tumorigenesis and metastasis by cisplatin
    Article Snippet: The RNA was first fragmented into small pieces and then cDNA libraries were prepared according to the manufacturer's instruction (Illumina Inc., USA) and purified by the QIAquick PCR Purification Kit (Qiagen). .. Polymerase chain reaction (PCR) was performed to amplify the cDNA library by using Gex PCR primers according to the manufacturer's protocol.

    Activated Clotting Time Assay:

    Article Title: Immune Failure in the Absence of Profound CD4+ T-Lymphocyte Depletion in Simian Immunodeficiency Virus-Infected Rapid Progressor Macaques
    Article Snippet: Verification was achieved by direct sequencing of PCR products (QIAquick PCR purification kit, Qiagen) by automated sequencing on the ABI 377 sequencer. .. The ability of herpesvirus papio-transformed B lymphoblastoid cell lines (B-LCL) from these macaques to act as targets in p11C-specific functional cytotoxicity assays was used to confirm the expression of the Mamu-A*01 allele.

    Article Title: Regime Shift and Microbial Dynamics in a Sequencing Batch Reactor for Nitrification and Anammox Treatment of Urine ▿Regime Shift and Microbial Dynamics in a Sequencing Batch Reactor for Nitrification and Anammox Treatment of Urine ▿ †
    Article Snippet: Successful PCR products were purified using a QIAquick PCR purification kit (Qiagen), and commercial sequencing was performed by Microsynth (Balgach, Switzerland). .. Nearly full-length 16S rRNA gene amplicons from the sample obtained on 25 September 2007 were obtained by PCR using general bacterial primers 27F (3′-AGA GTT TGA TCM TGG CTC AG-5′) and 1492R (TAC GGY TAC CTT GTT ACG ACT T) ( ).

    Real-time Polymerase Chain Reaction:

    Article Title: TFIIH Subunit Alterations Causing Xeroderma Pigmentosum and Trichothiodystrophy Specifically Disturb Several Steps during Transcription
    Article Snippet: .. DNA fragments were purified with the QIAquick PCR Purification Kit (QIAGEN) and analyzed by qPCR with a set of primers targeting the promoter and terminator regions of RARB2 ( ). .. In parallel, cross-linked cells were permeabilized with cytonin (Trevigen) for 30 min at RT.

    Article Title: Analysis of chromatin binding dynamics using the crosslinking kinetics (CLK) method
    Article Snippet: Paragraph title: Step 3: Quantification of ChIP DNA using real time PCR ... The following day, all samples are cleaned up with the QIAquick PCR purification kit (Qiagen) according to the manufacturer’s instructions and the purified DNA is eluted from the columns with 50 µl of 50°C DEPC-treated water.

    Chromatin Immunoprecipitation:

    Article Title: TFIIH Subunit Alterations Causing Xeroderma Pigmentosum and Trichothiodystrophy Specifically Disturb Several Steps during Transcription
    Article Snippet: Paragraph title: Chromatin Immunoprecipitation and BIOTIN-ChIP Assays ... DNA fragments were purified with the QIAquick PCR Purification Kit (QIAGEN) and analyzed by qPCR with a set of primers targeting the promoter and terminator regions of RARB2 ( ).

    Article Title: Analysis of chromatin binding dynamics using the crosslinking kinetics (CLK) method
    Article Snippet: Paragraph title: Step 3: Quantification of ChIP DNA using real time PCR ... The following day, all samples are cleaned up with the QIAquick PCR purification kit (Qiagen) according to the manufacturer’s instructions and the purified DNA is eluted from the columns with 50 µl of 50°C DEPC-treated water.

    Plasmid Preparation:

    Article Title: Integron-Containing IncU R Plasmids pRAS1 and pAr-32 from the Fish Pathogen Aeromonas salmonicida
    Article Snippet: Template DNA either was isolated plasmid DNA or was from boiled bacterial suspensions. .. PCR amplicons were purified by use of the Qiaquick PCR purification kit (Qiagen GmbH), the reaction mixtures for the sequencing PCR were prepared, and the products were purified as recommended in the protocol of the BigDye Terminator Ready Reaction kit (Applied Biosystems, Warrington, United Kingdom).

    Article Title: Arsenic(V) Reduction in Relation to Iron(III) Transformation and Molecular Characterization of the Structural and Functional Microbial Community in Sediments of a Basin-Fill Aquifer in Northern Utah
    Article Snippet: .. Amplified products (equal concentrations of DNA) from the three replicates of each treatment were pooled, purified with the QIAquick PCR purification kit (Qiagen, Valencia, CA), ligated into the TOPO TA pCR4.0 vector, and transformed into Escherichia coli One Shot TOP10 competent cells (TOPO TA cloning kit; Life Technologies, CA) as recommended by the manufacturer. .. A total of six independent clone libraries were generated and a minimum of 45 arrA gene clones were sequenced per treatment (total of 288 sequences).

    Article Title: Germ Line Variants of Human N-Methylpurine DNA Glycosylase Show Impaired DNA Repair Activity and Facilitate 1,N6-Ethenoadenine-induced Mutations *
    Article Snippet: .. Finally, the reaction was incubated with 1× Supercoil-It buffer (Bayou Biolabs, Metairie, LA) and Supercoil-It enzyme at 37 °C for 3 h. Plasmid DNA was recovered using the QIAquick PCR Purification kit (Qiagen). .. The damaged (ϵA and 8-oxo-dG) constructs were transfected into cells with Lipofectamine 2000 (Invitrogen) following the manufacturer's protocol.

    Article Title: Sequence-Based Optimization of a Quantitative Real-Time PCR Assay for Detection of Plasmodium ovale and Plasmodium malariae
    Article Snippet: PCR products were purified with a QIAquick PCR purification kit (Qiagen, Germantown, MD) and eluted with 20 μl water. .. Forward and reverse sequences of each sample were aligned using Vector NTI software (Life Technologies).

    Article Title: Use of Inverse PCR for Analysis of Class 1 Integrons Carrying an Unusual 3? Conserved Segment Structure ▿
    Article Snippet: Finally, the inverse PCR products were purified using the QIAquick PCR purification kit (Qiagen Inc., Valencia, California) for primer-walking-strategy DNA sequencing and then analyzed using the BLAST suite of programs. .. Plasmid DNA was isolated using an alkaline lysis method ( ).

    Software:

    Article Title: Integron-Containing IncU R Plasmids pRAS1 and pAr-32 from the Fish Pathogen Aeromonas salmonicida
    Article Snippet: PCR amplicons were purified by use of the Qiaquick PCR purification kit (Qiagen GmbH), the reaction mixtures for the sequencing PCR were prepared, and the products were purified as recommended in the protocol of the BigDye Terminator Ready Reaction kit (Applied Biosystems, Warrington, United Kingdom). .. The products from the sequencing PCR were separated with an ABI Prism 377 automated sequencing machine (Perkin-Elmer Cetus Corp., Foster City, Calif.), and the DNA sequences were analyzed by using the GCG Sequence Analysis software package (version 8; Genetics Computer Group, Madison, Wis.) and BLAST search analysis ( ).

    Article Title: Regime Shift and Microbial Dynamics in a Sequencing Batch Reactor for Nitrification and Anammox Treatment of Urine ▿Regime Shift and Microbial Dynamics in a Sequencing Batch Reactor for Nitrification and Anammox Treatment of Urine ▿ †
    Article Snippet: Successful PCR products were purified using a QIAquick PCR purification kit (Qiagen), and commercial sequencing was performed by Microsynth (Balgach, Switzerland). .. DGGE patterns were analyzed using the Quantity One software package (Bio-Rad).

    Article Title: Sequence-Based Optimization of a Quantitative Real-Time PCR Assay for Detection of Plasmodium ovale and Plasmodium malariae
    Article Snippet: PCR products were purified with a QIAquick PCR purification kit (Qiagen, Germantown, MD) and eluted with 20 μl water. .. Forward and reverse sequences of each sample were aligned using Vector NTI software (Life Technologies).

    Electrophoresis:

    Article Title: Integron-Containing IncU R Plasmids pRAS1 and pAr-32 from the Fish Pathogen Aeromonas salmonicida
    Article Snippet: The PCR products were separated by electrophoresis in 0.7% agarose gels in a horizontal gel apparatus. .. PCR amplicons were purified by use of the Qiaquick PCR purification kit (Qiagen GmbH), the reaction mixtures for the sequencing PCR were prepared, and the products were purified as recommended in the protocol of the BigDye Terminator Ready Reaction kit (Applied Biosystems, Warrington, United Kingdom).

    Functional Assay:

    Article Title: Arsenic(V) Reduction in Relation to Iron(III) Transformation and Molecular Characterization of the Structural and Functional Microbial Community in Sediments of a Basin-Fill Aquifer in Northern Utah
    Article Snippet: Paragraph title: Functional gene ( arrA ) cloning and DNA sequencing. ... Amplified products (equal concentrations of DNA) from the three replicates of each treatment were pooled, purified with the QIAquick PCR purification kit (Qiagen, Valencia, CA), ligated into the TOPO TA pCR4.0 vector, and transformed into Escherichia coli One Shot TOP10 competent cells (TOPO TA cloning kit; Life Technologies, CA) as recommended by the manufacturer.

    Article Title: Immune Failure in the Absence of Profound CD4+ T-Lymphocyte Depletion in Simian Immunodeficiency Virus-Infected Rapid Progressor Macaques
    Article Snippet: Verification was achieved by direct sequencing of PCR products (QIAquick PCR purification kit, Qiagen) by automated sequencing on the ABI 377 sequencer. .. The ability of herpesvirus papio-transformed B lymphoblastoid cell lines (B-LCL) from these macaques to act as targets in p11C-specific functional cytotoxicity assays was used to confirm the expression of the Mamu-A*01 allele.

    Denaturing Gradient Gel Electrophoresis:

    Article Title: Regime Shift and Microbial Dynamics in a Sequencing Batch Reactor for Nitrification and Anammox Treatment of Urine ▿Regime Shift and Microbial Dynamics in a Sequencing Batch Reactor for Nitrification and Anammox Treatment of Urine ▿ †
    Article Snippet: DGGE was performed using general bacterial primers 341F-GC and 534R as described in reference using a DCode DGGE system (Bio-Rad Laboratories AG, Reinach, Switzerland) for gel electrophoresis. .. Successful PCR products were purified using a QIAquick PCR purification kit (Qiagen), and commercial sequencing was performed by Microsynth (Balgach, Switzerland).

    In Vitro:

    Article Title: West Nile virus (WNV) genome RNAs with up to three adjacent mutations that disrupt long distance 5?-3? cyclization sequence basepairs are viable
    Article Snippet: Paragraph title: Transfection of in vitro transcribed viral RNA and assay of progeny virus replication ... Parental or mutated infectious clone DNA was linearized by digestion with XbaI and then purified using a Qiaquick PCR Purification kit (Qiagen).

    Article Title: Germ Line Variants of Human N-Methylpurine DNA Glycosylase Show Impaired DNA Repair Activity and Facilitate 1,N6-Ethenoadenine-induced Mutations *
    Article Snippet: Paragraph title: ϵA or 8-Oxo-dG pBS-SV40 in Vitro Construct Preparation ... Finally, the reaction was incubated with 1× Supercoil-It buffer (Bayou Biolabs, Metairie, LA) and Supercoil-It enzyme at 37 °C for 3 h. Plasmid DNA was recovered using the QIAquick PCR Purification kit (Qiagen).

    Knock-Out:

    Article Title: Dihydropteroate Synthase of Mycobacterium leprae and Dapsone Resistance
    Article Snippet: Paragraph title: Cloning of folP homologs and complementation of folP knockout mutants. ... The resultant PCR fragments were purified and concentrated using a QIAQuick PCR Purification Kit (QIAGEN, Valencia, Calif.), and DNA sequences were obtained by automated sequencing on a PE BioSystems 377 automated DNA sequencer (Perkin-Elmer, Gaithersburg, Md.).

    Immunoprecipitation:

    Article Title: TFIIH Subunit Alterations Causing Xeroderma Pigmentosum and Trichothiodystrophy Specifically Disturb Several Steps during Transcription
    Article Snippet: Samples were immunoprecipitated with antibodies at 4°C overnight, and protein G Sepharose beads (Upstate) were added, incubated for 4 hr at 4°C, and sequentially washed. .. DNA fragments were purified with the QIAquick PCR Purification Kit (QIAGEN) and analyzed by qPCR with a set of primers targeting the promoter and terminator regions of RARB2 ( ).

    Alkaline Lysis:

    Article Title: Use of Inverse PCR for Analysis of Class 1 Integrons Carrying an Unusual 3? Conserved Segment Structure ▿
    Article Snippet: Finally, the inverse PCR products were purified using the QIAquick PCR purification kit (Qiagen Inc., Valencia, California) for primer-walking-strategy DNA sequencing and then analyzed using the BLAST suite of programs. .. Plasmid DNA was isolated using an alkaline lysis method ( ).

    Recombinant:

    Article Title: TFIIH Subunit Alterations Causing Xeroderma Pigmentosum and Trichothiodystrophy Specifically Disturb Several Steps during Transcription
    Article Snippet: DNA fragments were purified with the QIAquick PCR Purification Kit (QIAGEN) and analyzed by qPCR with a set of primers targeting the promoter and terminator regions of RARB2 ( ). .. After extensive washes with PBS, a terminal deoxynucleotidyl transferase (TdT) reaction was performed with Biotin-16-dUTP (Roche) and 60 units of recombinant enzyme rTdT (Promega).

    Article Title: Dihydropteroate Synthase of Mycobacterium leprae and Dapsone Resistance
    Article Snippet: E. coli XL-1 Blue cells were transformed with these plasmids, and recombinant clones were selected on LB agar containing 100 μg of ampicillin/ml. .. The resultant PCR fragments were purified and concentrated using a QIAQuick PCR Purification Kit (QIAGEN, Valencia, Calif.), and DNA sequences were obtained by automated sequencing on a PE BioSystems 377 automated DNA sequencer (Perkin-Elmer, Gaithersburg, Md.).

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    Qiagen qia quick pcr purification kit
    Qia Quick Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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