qia quick qiagen pcr purification kit  (Qiagen)


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    Name:
    QIAquick PCR Purification Kit
    Description:
    For purification of up to 10 μg PCR products 100 bp to 10 kb Kit contents Qiagen QIAquick PCR Purification Kit 50 rxns 30L Elution Volume 10g Binding Capacity Tube Format Manual Processing Silica Technology 100 bp to 10 kb Fragment 40mers Fragments Removed Ideal for Sequencing Microarray Analysis Ligation and Transformation Restriction Digestion Labeling Microinjection PCR and in vitro Transcription For Purification of up to 10μg PCR Products Includes 50 QIAquick Spin Columns Buffers 2mL Collection Tubes Benefits Up to 95 recovery of ready to use DNA Cleanup of DNA up to 10 kb in three easy steps Gel loading dye for convenient sample analysis
    Catalog Number:
    28104
    Price:
    117
    Category:
    QIAquick PCR Purification Kit
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    Structured Review

    Qiagen qia quick qiagen pcr purification kit
    QIAquick PCR Purification Kit
    For purification of up to 10 μg PCR products 100 bp to 10 kb Kit contents Qiagen QIAquick PCR Purification Kit 50 rxns 30L Elution Volume 10g Binding Capacity Tube Format Manual Processing Silica Technology 100 bp to 10 kb Fragment 40mers Fragments Removed Ideal for Sequencing Microarray Analysis Ligation and Transformation Restriction Digestion Labeling Microinjection PCR and in vitro Transcription For Purification of up to 10μg PCR Products Includes 50 QIAquick Spin Columns Buffers 2mL Collection Tubes Benefits Up to 95 recovery of ready to use DNA Cleanup of DNA up to 10 kb in three easy steps Gel loading dye for convenient sample analysis
    https://www.bioz.com/result/qia quick qiagen pcr purification kit/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qia quick qiagen pcr purification kit - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics
    Article Snippet: .. RPA products from crude extracts could be cloned if the amplified DNA was cleaned with QIAquick PCR purification columns. .. RPA amplicons reactions denatured at 65 °C for 10 min could be digested with Cla I or cloned if centrifuged at 3800 rcf for 20 s before restriction digestion or cloning.

    Centrifugation:

    Article Title: Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits
    Article Snippet: Kit extractions We tested three different silica-column kits: Zymo ZR Viral DNA/RNA Kit (outdated protocol, D7021), Zymo Quick-DNA/RNA Kit (updated protocol, D7021), and the QIAquick PCR Purification Kit (28104, Qiagen). .. For all silica-column kits, fresh collection tubes were used after each spin and centrifugation speeds were set to 16,000 × g. Centrifugation was performed on either an Eppendorf 5415D centrifuge (Eppendorf, Hauppauge, NY, USA) or a Thermo Fisher Scientific AccuSpin Micro 17 R centrifuge (13–100–676).

    Amplification:

    Article Title: Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics
    Article Snippet: .. RPA products from crude extracts could be cloned if the amplified DNA was cleaned with QIAquick PCR purification columns. .. RPA amplicons reactions denatured at 65 °C for 10 min could be digested with Cla I or cloned if centrifuged at 3800 rcf for 20 s before restriction digestion or cloning.

    Article Title: Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics
    Article Snippet: .. Proteins were separated from the amplified DNA (“cleaned”) with either QIAquick PCR purification columns (Qiagen, Valencia, CA) or using one of the methods described below. .. Unless otherwise stated, RPA products were denatured at 65 °C for 10 min before 10 μl were loaded into wells of 1.5 % agarose gels containing ethidium bromide.

    TA Cloning:

    Article Title: Epigenetic Regulation of HYAL-1 Hyaluronidase Expression
    Article Snippet: TOPO TA Cloning® kit (pCR®2.1-TOPO® vector), Superscript® II reverse transcription kit, and Platinum® Taq DNA polymerase (high fidelity) were purchased from Invitrogen. .. Genomic DNA isolation kit, QIAquick® PCR purification kit, EpiTect®Bisulfite kit, and Effectene® transfection reagent were from Qiagen (Valencia, CA).

    Blocking Assay:

    Article Title: Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics
    Article Snippet: Reactions were incubated at 37 °C for 30 min using a water bath, heating block or thermocycler. .. Proteins were separated from the amplified DNA (“cleaned”) with either QIAquick PCR purification columns (Qiagen, Valencia, CA) or using one of the methods described below.

    Real-time Polymerase Chain Reaction:

    Article Title: VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolism
    Article Snippet: .. DNA was purified with a PCR purification kit (#28104, Qiagen). qPCR was performed with special primers (Supplementary Table ) flanking the putative Dnmt3a- and STAT3-binding sites. .. The input DNA was an aliquot of sheared chromatin before immunoprecipitation and was used for normalization of the samples to the amount of chromatin added to each ChIP.

    Incubation:

    Article Title: Specific functions of TET1 and TET2 in regulating mesenchymal cell lineage determination
    Article Snippet: The immunoprecipitation was incubated with magnetic IgG beads for 2 h at 4 °C and washed with IP buffer and then in high salt buffer (10 mM sodium phosphate (pH 7.0), 400 mM NaCl, 0.05% Triton X-100). .. Immunoprecipitated bead–DNA complex was treated with proteinase K for 3 h at 50 °C in elution buffer and hydroxymethylated DNA purified by using the Qiagen PCR clean-up kit (Qiaquick).

    Article Title: Streamlined procedure for gene knockouts using all-in-one adenoviral CRISPR-Cas9
    Article Snippet: 35 ul 8 M potassium acetate was added and incubated at room temperature for 5 minutes. .. Amplicons were confirmed by agarose electrophoresis, and purified with PCR purification kit (QIAGEN Cat.28104).

    Article Title: Critical Parameters for Efficient Sonication and Improved Chromatin Immunoprecipitation of High Molecular Weight Proteins
    Article Snippet: .. In the second procedure, referred to as the “quick” procedure, samples were incubated for 1 h at +60°C, and the resulting DNA was purified with a PCR purification kit (Qiagen, 28104). ..

    Article Title: Enhancing mammary differentiation by overcoming lineage-specific epigenetic modification and signature gene expression of fibroblast-derived iPSCs
    Article Snippet: The cells were centrifuged briefly and resuspended in 100 μ l of buffer B (Diagenode LowCell ChIP Kit, Diagenode, Liego, Belgium) containing 1 mM PMSF and 0.1% protease inhibitor cocktail, incubated for 10 min on ice, and then sonicated (S-4000 sonicator; Misonix Inc., Farmingdale, NY, USA) at an amplitude of 99, pulse on 30 s, and pulse off 30 s for 15–25 min. .. Input DNA purification was processed with a PCR Purification Kit (Qiagen; cat. no. 28106).

    Article Title: Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics
    Article Snippet: Reactions were incubated at 37 °C for 30 min using a water bath, heating block or thermocycler. .. Proteins were separated from the amplified DNA (“cleaned”) with either QIAquick PCR purification columns (Qiagen, Valencia, CA) or using one of the methods described below.

    Luciferase:

    Article Title: Epigenetic Regulation of HYAL-1 Hyaluronidase Expression
    Article Snippet: Human Sure-RACE™ cDNA 5′ end discovery panel was purchased from Origene Technologies Inc. (Rockville, MD). pGL4.10[ luc2 ] vector, pGL4.74[hRluc/TK] vector, and Dual-Glo™ luciferase assay system were purchased from Promega Corp. (Madison, WI). .. Genomic DNA isolation kit, QIAquick® PCR purification kit, EpiTect®Bisulfite kit, and Effectene® transfection reagent were from Qiagen (Valencia, CA).

    Recombinase Polymerase Amplification:

    Article Title: Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics
    Article Snippet: .. RPA products from crude extracts could be cloned if the amplified DNA was cleaned with QIAquick PCR purification columns. .. RPA amplicons reactions denatured at 65 °C for 10 min could be digested with Cla I or cloned if centrifuged at 3800 rcf for 20 s before restriction digestion or cloning.

    Article Title: Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics
    Article Snippet: Paragraph title: RPA assays ... Proteins were separated from the amplified DNA (“cleaned”) with either QIAquick PCR purification columns (Qiagen, Valencia, CA) or using one of the methods described below.

    Transfection:

    Article Title: Streamlined procedure for gene knockouts using all-in-one adenoviral CRISPR-Cas9
    Article Snippet: Paragraph title: Transfection and T7E1 assay ... Amplicons were confirmed by agarose electrophoresis, and purified with PCR purification kit (QIAGEN Cat.28104).

    Article Title: Epigenetic Regulation of HYAL-1 Hyaluronidase Expression
    Article Snippet: .. Genomic DNA isolation kit, QIAquick® PCR purification kit, EpiTect®Bisulfite kit, and Effectene® transfection reagent were from Qiagen (Valencia, CA). .. MG132 was purchased from EMD Biosciences Inc. (San Diego, CA), and human genomic DNA was obtained from Clontech.

    Protease Inhibitor:

    Article Title: Enhancing mammary differentiation by overcoming lineage-specific epigenetic modification and signature gene expression of fibroblast-derived iPSCs
    Article Snippet: The cells were centrifuged briefly and resuspended in 100 μ l of buffer B (Diagenode LowCell ChIP Kit, Diagenode, Liego, Belgium) containing 1 mM PMSF and 0.1% protease inhibitor cocktail, incubated for 10 min on ice, and then sonicated (S-4000 sonicator; Misonix Inc., Farmingdale, NY, USA) at an amplitude of 99, pulse on 30 s, and pulse off 30 s for 15–25 min. .. Input DNA purification was processed with a PCR Purification Kit (Qiagen; cat. no. 28106).

    Infection:

    Article Title: Rapid Phenotypic Characterization Method for Herpes Simplex Virus and Varicella-Zoster Virus Thymidine Kinases To Screen for Acyclovir-Resistant Viral Infection
    Article Snippet: PCR was performed with 100 μl of reaction mixture containing 1 μl of the sample DNA, which corresponded to approximately 10 infected cells, using an Expand Hi-Fi PCR system (Roche Molecular Systems). .. The PCR products were purified and dissolved in 30 μl of 10 mM Tris-HCl, pH 8.5, with a QIAquick PCR Purification Kit (Qiagen, Hilden, Germany), and stored at −20°C.

    Polymerase Chain Reaction:

    Article Title: Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics
    Article Snippet: .. RPA products from crude extracts could be cloned if the amplified DNA was cleaned with QIAquick PCR purification columns. .. RPA amplicons reactions denatured at 65 °C for 10 min could be digested with Cla I or cloned if centrifuged at 3800 rcf for 20 s before restriction digestion or cloning.

    Article Title: Specific functions of TET1 and TET2 in regulating mesenchymal cell lineage determination
    Article Snippet: .. Immunoprecipitated bead–DNA complex was treated with proteinase K for 3 h at 50 °C in elution buffer and hydroxymethylated DNA purified by using the Qiagen PCR clean-up kit (Qiaquick). ..

    Article Title: Streamlined procedure for gene knockouts using all-in-one adenoviral CRISPR-Cas9
    Article Snippet: .. Amplicons were confirmed by agarose electrophoresis, and purified with PCR purification kit (QIAGEN Cat.28104). .. 200 ng DNA samples were denatured and annealed according to the protocol in Ran et al . and subjected to T7E1 digestion in a 20 ul reaction according to the manufacturer’s protocol (NEB cat.M0302).

    Article Title: Critical Parameters for Efficient Sonication and Improved Chromatin Immunoprecipitation of High Molecular Weight Proteins
    Article Snippet: .. In the second procedure, referred to as the “quick” procedure, samples were incubated for 1 h at +60°C, and the resulting DNA was purified with a PCR purification kit (Qiagen, 28104). ..

    Article Title: Epigenetic Regulation of HYAL-1 Hyaluronidase Expression
    Article Snippet: .. Genomic DNA isolation kit, QIAquick® PCR purification kit, EpiTect®Bisulfite kit, and Effectene® transfection reagent were from Qiagen (Valencia, CA). .. MG132 was purchased from EMD Biosciences Inc. (San Diego, CA), and human genomic DNA was obtained from Clontech.

    Article Title: Rapid Phenotypic Characterization Method for Herpes Simplex Virus and Varicella-Zoster Virus Thymidine Kinases To Screen for Acyclovir-Resistant Viral Infection
    Article Snippet: .. The PCR products were purified and dissolved in 30 μl of 10 mM Tris-HCl, pH 8.5, with a QIAquick PCR Purification Kit (Qiagen, Hilden, Germany), and stored at −20°C. .. TK polypeptides of the HSV and VZV strains were synthesized directly from TK genes amplified with PCR using a Single Tube Protein System 3 (Novagen, Madison, Wis.) in accordance with the manufacturer's instructions.

    Article Title: Use of a restriction enzyme-digested PCR product as substrate for helicase assays
    Article Snippet: .. Following PCR, the product was purified using QIAquick PCR purification kit (Qiagen) and digested with restriction enzymes that generate either a 3′ or a 5′ 4-base ssDNA overhang. ..

    Article Title: Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits
    Article Snippet: .. Kit extractions We tested three different silica-column kits: Zymo ZR Viral DNA/RNA Kit (outdated protocol, D7021), Zymo Quick-DNA/RNA Kit (updated protocol, D7021), and the QIAquick PCR Purification Kit (28104, Qiagen). .. For all silica-column kits, fresh collection tubes were used after each spin and centrifugation speeds were set to 16,000 × g. Centrifugation was performed on either an Eppendorf 5415D centrifuge (Eppendorf, Hauppauge, NY, USA) or a Thermo Fisher Scientific AccuSpin Micro 17 R centrifuge (13–100–676).

    Article Title: Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits
    Article Snippet: .. For the QIAquick PCR Purification Kit, 125 µL sample was mixed with 625 µL Buffer PB without indicator. .. 750 µL lysed sample was centrifuged for 30 s, followed by 750 µL Buffer PE for 30 s, a dry spin for 1 min, and 50 µL nuclease-free water for 1 min. Optionally, the dry spin was skipped or the dry spin was replaced with a 300 µL TPW and centrifuged for 1 min. We tested the Zymo Quick-DNA/RNA Viral MagBead kit (R2140).

    Article Title: Enhancing mammary differentiation by overcoming lineage-specific epigenetic modification and signature gene expression of fibroblast-derived iPSCs
    Article Snippet: .. Input DNA purification was processed with a PCR Purification Kit (Qiagen; cat. no. 28106). .. The primers used for the ChIP PCR are listed below.

    Article Title: Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics
    Article Snippet: .. Proteins were separated from the amplified DNA (“cleaned”) with either QIAquick PCR purification columns (Qiagen, Valencia, CA) or using one of the methods described below. .. Unless otherwise stated, RPA products were denatured at 65 °C for 10 min before 10 μl were loaded into wells of 1.5 % agarose gels containing ethidium bromide.

    Article Title: VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolism
    Article Snippet: .. DNA was purified with a PCR purification kit (#28104, Qiagen). qPCR was performed with special primers (Supplementary Table ) flanking the putative Dnmt3a- and STAT3-binding sites. .. The input DNA was an aliquot of sheared chromatin before immunoprecipitation and was used for normalization of the samples to the amount of chromatin added to each ChIP.

    Sonication:

    Article Title: Specific functions of TET1 and TET2 in regulating mesenchymal cell lineage determination
    Article Snippet: Genomic DNA was prepared using the Qiagen genomic DNA isolation kit and then sonicated to generate 200–500 bp fragments. .. Immunoprecipitated bead–DNA complex was treated with proteinase K for 3 h at 50 °C in elution buffer and hydroxymethylated DNA purified by using the Qiagen PCR clean-up kit (Qiaquick).

    Article Title: Critical Parameters for Efficient Sonication and Improved Chromatin Immunoprecipitation of High Molecular Weight Proteins
    Article Snippet: Analysis of DNA Fragmentation 10–30 μL of the sonicated chromatin samples were combined with 10 μL of 10 mg/mL Proteinase K (Sigma, P2308), 50 μL of 2x reverse cross-linking master mix (100 mM Tris pH 8.0, 600 mM NaCl, 1.0% SDS, 100 mM EDTA pH 8.0; kept at +37°C to avoid SDS precipitation) and brought to a final volume of 100 μL with water. .. In the second procedure, referred to as the “quick” procedure, samples were incubated for 1 h at +60°C, and the resulting DNA was purified with a PCR purification kit (Qiagen, 28104).

    Article Title: Enhancing mammary differentiation by overcoming lineage-specific epigenetic modification and signature gene expression of fibroblast-derived iPSCs
    Article Snippet: The cells were centrifuged briefly and resuspended in 100 μ l of buffer B (Diagenode LowCell ChIP Kit, Diagenode, Liego, Belgium) containing 1 mM PMSF and 0.1% protease inhibitor cocktail, incubated for 10 min on ice, and then sonicated (S-4000 sonicator; Misonix Inc., Farmingdale, NY, USA) at an amplitude of 99, pulse on 30 s, and pulse off 30 s for 15–25 min. .. Input DNA purification was processed with a PCR Purification Kit (Qiagen; cat. no. 28106).

    DNA Extraction:

    Article Title: Specific functions of TET1 and TET2 in regulating mesenchymal cell lineage determination
    Article Snippet: Genomic DNA was prepared using the Qiagen genomic DNA isolation kit and then sonicated to generate 200–500 bp fragments. .. Immunoprecipitated bead–DNA complex was treated with proteinase K for 3 h at 50 °C in elution buffer and hydroxymethylated DNA purified by using the Qiagen PCR clean-up kit (Qiaquick).

    Article Title: Epigenetic Regulation of HYAL-1 Hyaluronidase Expression
    Article Snippet: .. Genomic DNA isolation kit, QIAquick® PCR purification kit, EpiTect®Bisulfite kit, and Effectene® transfection reagent were from Qiagen (Valencia, CA). .. MG132 was purchased from EMD Biosciences Inc. (San Diego, CA), and human genomic DNA was obtained from Clontech.

    Labeling:

    Article Title: Use of a restriction enzyme-digested PCR product as substrate for helicase assays
    Article Snippet: A PCR reaction was performed with either 32 P-labeled primer (only one primer labeled) or in the presence of 32 P-dNTPs (see Materials and Methods). .. Following PCR, the product was purified using QIAquick PCR purification kit (Qiagen) and digested with restriction enzymes that generate either a 3′ or a 5′ 4-base ssDNA overhang.

    Purification:

    Article Title: Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics
    Article Snippet: .. RPA products from crude extracts could be cloned if the amplified DNA was cleaned with QIAquick PCR purification columns. .. RPA amplicons reactions denatured at 65 °C for 10 min could be digested with Cla I or cloned if centrifuged at 3800 rcf for 20 s before restriction digestion or cloning.

    Article Title: Specific functions of TET1 and TET2 in regulating mesenchymal cell lineage determination
    Article Snippet: .. Immunoprecipitated bead–DNA complex was treated with proteinase K for 3 h at 50 °C in elution buffer and hydroxymethylated DNA purified by using the Qiagen PCR clean-up kit (Qiaquick). ..

    Article Title: Streamlined procedure for gene knockouts using all-in-one adenoviral CRISPR-Cas9
    Article Snippet: .. Amplicons were confirmed by agarose electrophoresis, and purified with PCR purification kit (QIAGEN Cat.28104). .. 200 ng DNA samples were denatured and annealed according to the protocol in Ran et al . and subjected to T7E1 digestion in a 20 ul reaction according to the manufacturer’s protocol (NEB cat.M0302).

    Article Title: Critical Parameters for Efficient Sonication and Improved Chromatin Immunoprecipitation of High Molecular Weight Proteins
    Article Snippet: .. In the second procedure, referred to as the “quick” procedure, samples were incubated for 1 h at +60°C, and the resulting DNA was purified with a PCR purification kit (Qiagen, 28104). ..

    Article Title: Epigenetic Regulation of HYAL-1 Hyaluronidase Expression
    Article Snippet: .. Genomic DNA isolation kit, QIAquick® PCR purification kit, EpiTect®Bisulfite kit, and Effectene® transfection reagent were from Qiagen (Valencia, CA). .. MG132 was purchased from EMD Biosciences Inc. (San Diego, CA), and human genomic DNA was obtained from Clontech.

    Article Title: Rapid Phenotypic Characterization Method for Herpes Simplex Virus and Varicella-Zoster Virus Thymidine Kinases To Screen for Acyclovir-Resistant Viral Infection
    Article Snippet: .. The PCR products were purified and dissolved in 30 μl of 10 mM Tris-HCl, pH 8.5, with a QIAquick PCR Purification Kit (Qiagen, Hilden, Germany), and stored at −20°C. .. TK polypeptides of the HSV and VZV strains were synthesized directly from TK genes amplified with PCR using a Single Tube Protein System 3 (Novagen, Madison, Wis.) in accordance with the manufacturer's instructions.

    Article Title: Use of a restriction enzyme-digested PCR product as substrate for helicase assays
    Article Snippet: .. Following PCR, the product was purified using QIAquick PCR purification kit (Qiagen) and digested with restriction enzymes that generate either a 3′ or a 5′ 4-base ssDNA overhang. ..

    Article Title: Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits
    Article Snippet: .. Kit extractions We tested three different silica-column kits: Zymo ZR Viral DNA/RNA Kit (outdated protocol, D7021), Zymo Quick-DNA/RNA Kit (updated protocol, D7021), and the QIAquick PCR Purification Kit (28104, Qiagen). .. For all silica-column kits, fresh collection tubes were used after each spin and centrifugation speeds were set to 16,000 × g. Centrifugation was performed on either an Eppendorf 5415D centrifuge (Eppendorf, Hauppauge, NY, USA) or a Thermo Fisher Scientific AccuSpin Micro 17 R centrifuge (13–100–676).

    Article Title: Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits
    Article Snippet: .. For the QIAquick PCR Purification Kit, 125 µL sample was mixed with 625 µL Buffer PB without indicator. .. 750 µL lysed sample was centrifuged for 30 s, followed by 750 µL Buffer PE for 30 s, a dry spin for 1 min, and 50 µL nuclease-free water for 1 min. Optionally, the dry spin was skipped or the dry spin was replaced with a 300 µL TPW and centrifuged for 1 min. We tested the Zymo Quick-DNA/RNA Viral MagBead kit (R2140).

    Article Title: Enhancing mammary differentiation by overcoming lineage-specific epigenetic modification and signature gene expression of fibroblast-derived iPSCs
    Article Snippet: .. Input DNA purification was processed with a PCR Purification Kit (Qiagen; cat. no. 28106). .. The primers used for the ChIP PCR are listed below.

    Article Title: Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics
    Article Snippet: .. Proteins were separated from the amplified DNA (“cleaned”) with either QIAquick PCR purification columns (Qiagen, Valencia, CA) or using one of the methods described below. .. Unless otherwise stated, RPA products were denatured at 65 °C for 10 min before 10 μl were loaded into wells of 1.5 % agarose gels containing ethidium bromide.

    Article Title: VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolism
    Article Snippet: .. DNA was purified with a PCR purification kit (#28104, Qiagen). qPCR was performed with special primers (Supplementary Table ) flanking the putative Dnmt3a- and STAT3-binding sites. .. The input DNA was an aliquot of sheared chromatin before immunoprecipitation and was used for normalization of the samples to the amount of chromatin added to each ChIP.

    IA:

    Article Title: Epigenetic Regulation of HYAL-1 Hyaluronidase Expression
    Article Snippet: Materials —IQ™ Supermix, HYAL-1, and actin PCR primers and 5′-FAM/3′-BHQ-labeled HYAL-1 and actin probes were purchased from Bio-Rad, Sigma Genosys, and Integrated DNA Technology (Coralville, IA), respectively. .. Genomic DNA isolation kit, QIAquick® PCR purification kit, EpiTect®Bisulfite kit, and Effectene® transfection reagent were from Qiagen (Valencia, CA).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Streamlined procedure for gene knockouts using all-in-one adenoviral CRISPR-Cas9
    Article Snippet: .. Amplicons were confirmed by agarose electrophoresis, and purified with PCR purification kit (QIAGEN Cat.28104). .. 200 ng DNA samples were denatured and annealed according to the protocol in Ran et al . and subjected to T7E1 digestion in a 20 ul reaction according to the manufacturer’s protocol (NEB cat.M0302).

    Chromatin Immunoprecipitation:

    Article Title: Enhancing mammary differentiation by overcoming lineage-specific epigenetic modification and signature gene expression of fibroblast-derived iPSCs
    Article Snippet: Paragraph title: ChIP analysis ... Input DNA purification was processed with a PCR Purification Kit (Qiagen; cat. no. 28106).

    Article Title: VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolism
    Article Snippet: Paragraph title: Chromatin immunoprecipitation and qPCR ... DNA was purified with a PCR purification kit (#28104, Qiagen). qPCR was performed with special primers (Supplementary Table ) flanking the putative Dnmt3a- and STAT3-binding sites.

    Plasmid Preparation:

    Article Title: Epigenetic Regulation of HYAL-1 Hyaluronidase Expression
    Article Snippet: TOPO TA Cloning® kit (pCR®2.1-TOPO® vector), Superscript® II reverse transcription kit, and Platinum® Taq DNA polymerase (high fidelity) were purchased from Invitrogen. .. Genomic DNA isolation kit, QIAquick® PCR purification kit, EpiTect®Bisulfite kit, and Effectene® transfection reagent were from Qiagen (Valencia, CA).

    Electrophoresis:

    Article Title: Streamlined procedure for gene knockouts using all-in-one adenoviral CRISPR-Cas9
    Article Snippet: .. Amplicons were confirmed by agarose electrophoresis, and purified with PCR purification kit (QIAGEN Cat.28104). .. 200 ng DNA samples were denatured and annealed according to the protocol in Ran et al . and subjected to T7E1 digestion in a 20 ul reaction according to the manufacturer’s protocol (NEB cat.M0302).

    Ethanol Precipitation:

    Article Title: Streamlined procedure for gene knockouts using all-in-one adenoviral CRISPR-Cas9
    Article Snippet: The samples were centrifuged at 13,000 rpm for 15 minutes and genomic DNA was purified by subjecting the supernatant to phenol-chloroform extraction and ethanol precipitation. .. Amplicons were confirmed by agarose electrophoresis, and purified with PCR purification kit (QIAGEN Cat.28104).

    Immunoprecipitation:

    Article Title: Specific functions of TET1 and TET2 in regulating mesenchymal cell lineage determination
    Article Snippet: .. Immunoprecipitated bead–DNA complex was treated with proteinase K for 3 h at 50 °C in elution buffer and hydroxymethylated DNA purified by using the Qiagen PCR clean-up kit (Qiaquick). ..

    Article Title: Enhancing mammary differentiation by overcoming lineage-specific epigenetic modification and signature gene expression of fibroblast-derived iPSCs
    Article Snippet: The magnetic immunoprecipitation procedure followed the Diagenode LowCell ChIP Kit protocol (Diagenode; cat. no. Kch-mglow-G48). .. Input DNA purification was processed with a PCR Purification Kit (Qiagen; cat. no. 28106).

    Article Title: VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolism
    Article Snippet: Chromatin fragments were prepared, followed by immunoprecipitation with anti-Dnmt3a (#ab2850, Abcam, 1:1000, Rabbit), anti-STAT3 (#ab119352, Abcam, 1:1000, mouse) or rabbit/mosue IgG isotype mAbs and coupled to Dynabeads Protein G. DNA was phenol/chloroform extracted and ethanol precipitated in the presence of glycogen. .. DNA was purified with a PCR purification kit (#28104, Qiagen). qPCR was performed with special primers (Supplementary Table ) flanking the putative Dnmt3a- and STAT3-binding sites.

    DNA Purification:

    Article Title: Enhancing mammary differentiation by overcoming lineage-specific epigenetic modification and signature gene expression of fibroblast-derived iPSCs
    Article Snippet: .. Input DNA purification was processed with a PCR Purification Kit (Qiagen; cat. no. 28106). .. The primers used for the ChIP PCR are listed below.

    Staining:

    Article Title: Critical Parameters for Efficient Sonication and Improved Chromatin Immunoprecipitation of High Molecular Weight Proteins
    Article Snippet: In the second procedure, referred to as the “quick” procedure, samples were incubated for 1 h at +60°C, and the resulting DNA was purified with a PCR purification kit (Qiagen, 28104). .. Purified DNA was analysed on ethidium bromide stained agarose gels.

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    Qiagen qia quick pcr product purification kit
    Qia Quick Pcr Product Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qia quick pcr product purification kit/product/Qiagen
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    qia quick pcr product purification kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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