qia quick pcr purification kit  (Qiagen)


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    Structured Review

    Qiagen qia quick pcr purification kit
    Qia Quick Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qia quick pcr purification kit/product/Qiagen
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    qia quick pcr purification kit - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Complete genomic sequence of turkey coronavirus.
    Article Snippet: .. The PCR products were purified using the QIA quick PCR purification kit (Qiagen, Valencia, CA) and cloned in pGEM-T Easy (Promega, Madison, WI). .. Transformants were screened by restriction enzyme digestion and sequencing using primers specific for T7 and SP6 promoter.

    Amplification:

    Article Title: Complete genomic sequence of turkey coronavirus.
    Article Snippet: DNA cloning and sequence analysis Thirteen overlapped PCR fragments spanning the entire viral genome were amplified using specific primer sets (Table 1) . .. The PCR products were purified using the QIA quick PCR purification kit (Qiagen, Valencia, CA) and cloned in pGEM-T Easy (Promega, Madison, WI).

    In Vitro:

    Article Title: Absolute quantitation of infectious salmon anaemia virus using different real-time reverse transcription PCR chemistries.
    Article Snippet: Paragraph title: In vitro transcription of ISAV RNA segment 8 ... The linearized DNA was then purified using the QIA quick PCR purification kit (Qiagen), and was recovered in 30 l of elution buffer.

    Construct:

    Article Title: Complete genomic sequence of turkey coronavirus.
    Article Snippet: The PCR products were purified using the QIA quick PCR purification kit (Qiagen, Valencia, CA) and cloned in pGEM-T Easy (Promega, Madison, WI). .. The sequences were analyzed using the Sequencher 4.5 sequence analyses program, and a single contiguous sequence comprising the entire TCoV genome was constructed.

    Purification:

    Article Title: Complete genomic sequence of turkey coronavirus.
    Article Snippet: .. The PCR products were purified using the QIA quick PCR purification kit (Qiagen, Valencia, CA) and cloned in pGEM-T Easy (Promega, Madison, WI). .. Transformants were screened by restriction enzyme digestion and sequencing using primers specific for T7 and SP6 promoter.

    Article Title: Absolute quantitation of infectious salmon anaemia virus using different real-time reverse transcription PCR chemistries.
    Article Snippet: .. The linearized DNA was then purified using the QIA quick PCR purification kit (Qiagen), and was recovered in 30 l of elution buffer. .. In vitro transcription was carried out in a 40 l volume using 20 l of linearized plasmid DNA, 1× T7 RNA polymerase buffer, 2 l of 100 mM DTT, 16 l of 10 mM NTPs (Invitrogen), 1 l RNase OUT (40 U/l) (Invitrogen), and 1 l of T7 RNA polymerase (50 U/l) (Invitrogen).

    Polymerase Chain Reaction:

    Article Title: Complete genomic sequence of turkey coronavirus.
    Article Snippet: .. The PCR products were purified using the QIA quick PCR purification kit (Qiagen, Valencia, CA) and cloned in pGEM-T Easy (Promega, Madison, WI). .. Transformants were screened by restriction enzyme digestion and sequencing using primers specific for T7 and SP6 promoter.

    Article Title: Absolute quantitation of infectious salmon anaemia virus using different real-time reverse transcription PCR chemistries.
    Article Snippet: .. The linearized DNA was then purified using the QIA quick PCR purification kit (Qiagen), and was recovered in 30 l of elution buffer. .. In vitro transcription was carried out in a 40 l volume using 20 l of linearized plasmid DNA, 1× T7 RNA polymerase buffer, 2 l of 100 mM DTT, 16 l of 10 mM NTPs (Invitrogen), 1 l RNase OUT (40 U/l) (Invitrogen), and 1 l of T7 RNA polymerase (50 U/l) (Invitrogen).

    Incubation:

    Article Title: Absolute quantitation of infectious salmon anaemia virus using different real-time reverse transcription PCR chemistries.
    Article Snippet: The linearized DNA was then purified using the QIA quick PCR purification kit (Qiagen), and was recovered in 30 l of elution buffer. .. The reaction was incubated for 2 h at 37 • C. RNA purification was carried out using RNeasy kit (Qiagen), and was eluted in 30 l of nuclease-free water.

    Spectrophotometry:

    Article Title: Absolute quantitation of infectious salmon anaemia virus using different real-time reverse transcription PCR chemistries.
    Article Snippet: The linearized DNA was then purified using the QIA quick PCR purification kit (Qiagen), and was recovered in 30 l of elution buffer. .. Nucleic acid concentration was determined by UV spectrophotometry.

    Sequencing:

    Article Title: Complete genomic sequence of turkey coronavirus.
    Article Snippet: Paragraph title: DNA cloning and sequence analysis ... The PCR products were purified using the QIA quick PCR purification kit (Qiagen, Valencia, CA) and cloned in pGEM-T Easy (Promega, Madison, WI).

    Recombinant:

    Article Title: Absolute quantitation of infectious salmon anaemia virus using different real-time reverse transcription PCR chemistries.
    Article Snippet: For this, 200 ng of recombinant plasmid was linearized by digestion with BamHI enzyme (New England Biolabs) in a 20 l reaction volume following the manufacturer's protocol. .. The linearized DNA was then purified using the QIA quick PCR purification kit (Qiagen), and was recovered in 30 l of elution buffer.

    Nucleic Acid Concentration:

    Article Title: Absolute quantitation of infectious salmon anaemia virus using different real-time reverse transcription PCR chemistries.
    Article Snippet: The linearized DNA was then purified using the QIA quick PCR purification kit (Qiagen), and was recovered in 30 l of elution buffer. .. Nucleic acid concentration was determined by UV spectrophotometry.

    Plasmid Preparation:

    Article Title: Complete genomic sequence of turkey coronavirus.
    Article Snippet: The PCR products were purified using the QIA quick PCR purification kit (Qiagen, Valencia, CA) and cloned in pGEM-T Easy (Promega, Madison, WI). .. Prediction for ORFs was conducted using Vector NTI Advanced 10 (Invitrogen), and the sequences were analyzed using Lasergene DNA STAR (version 7, Lasergene Corp, Madison, WI).

    Article Title: Absolute quantitation of infectious salmon anaemia virus using different real-time reverse transcription PCR chemistries.
    Article Snippet: For this, 200 ng of recombinant plasmid was linearized by digestion with BamHI enzyme (New England Biolabs) in a 20 l reaction volume following the manufacturer's protocol. .. The linearized DNA was then purified using the QIA quick PCR purification kit (Qiagen), and was recovered in 30 l of elution buffer.

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  • News
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  • Team
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  • Bioz Stars
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  • 99
    Qiagen qia quick pcr purification kit
    Qia Quick Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qia quick pcr purification kit/product/Qiagen
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    qia quick pcr purification kit - by Bioz Stars, 2020-04
    99/100 stars
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