q5 polymerase reagents  (New England Biolabs)


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    Structured Review

    New England Biolabs q5 polymerase reagents
    Q5 Polymerase Reagents, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 polymerase reagents/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    q5 polymerase reagents - by Bioz Stars, 2020-04
    95/100 stars

    Related Products / Commonly Used Together

    transformants
    t7 primers

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    Related Articles

    Electroporation:

    Article Title: Cas9 Contributes to Group B Streptococcal Colonization and Disease
    Article Snippet: Transformants were confirmed using T3 and T7 primers and Q5 polymerase reagents (NEB). .. Positive transformants were maxi-prepped (Qiagen) and ∼1 mg/mL plasmid was transformed into competent GBS cells via electroporation at 1500 V and plating on THA plates containing erythromycin (5 μg/mL).

    Amplification:

    Article Title: Cas9 Contributes to Group B Streptococcal Colonization and Disease
    Article Snippet: Briefly, 5′ and 3′ flanking regions surrounding cas9 (RS04480 in GenBank accession NZ_HG939456.1), were amplified from COH1 genomic DNA and the chloramphenicol resistance encoding gene, cat , was amplified from COH1Δbspc ( ). .. Transformants were confirmed using T3 and T7 primers and Q5 polymerase reagents (NEB).

    Construct:

    Article Title: Cas9 Contributes to Group B Streptococcal Colonization and Disease
    Article Snippet: Deletion constructs and shuttle vector pHY304 ( ) were digested overnight with Sac II and Xho I, gel purified, ethanol precipitated, and ligated using Quick Ligase (NEB) according to the manufacturer’s protocol. .. Transformants were confirmed using T3 and T7 primers and Q5 polymerase reagents (NEB).

    Purification:

    Article Title: Cas9 Contributes to Group B Streptococcal Colonization and Disease
    Article Snippet: Deletion constructs and shuttle vector pHY304 ( ) were digested overnight with Sac II and Xho I, gel purified, ethanol precipitated, and ligated using Quick Ligase (NEB) according to the manufacturer’s protocol. .. Transformants were confirmed using T3 and T7 primers and Q5 polymerase reagents (NEB).

    Polymerase Chain Reaction:

    Article Title: Cas9 Contributes to Group B Streptococcal Colonization and Disease
    Article Snippet: Each Failsafe Premix contains 100 mM Tris–HCl (pH 8.3), 100 mM KCl, and 400 μM of each dNTP with varying concentrations of MgCl2 and FailSafe PCR Enhancer. .. Transformants were confirmed using T3 and T7 primers and Q5 polymerase reagents (NEB).

    Transformation Assay:

    Article Title: Cas9 Contributes to Group B Streptococcal Colonization and Disease
    Article Snippet: The ligated reaction was transformed in MC1061 E. coli according to the Mclab protocol, plated on LB containing erythromycin (500 μg/mL), and allowed to grow for 3–5 days at 30°C. .. Transformants were confirmed using T3 and T7 primers and Q5 polymerase reagents (NEB).

    Plasmid Preparation:

    Article Title: Cas9 Contributes to Group B Streptococcal Colonization and Disease
    Article Snippet: Deletion constructs and shuttle vector pHY304 ( ) were digested overnight with Sac II and Xho I, gel purified, ethanol precipitated, and ligated using Quick Ligase (NEB) according to the manufacturer’s protocol. .. Transformants were confirmed using T3 and T7 primers and Q5 polymerase reagents (NEB).

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    New England Biolabs q5 hot start high fidelity dna polymerase
    Q5 Hot Start High Fidelity Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 hot start high fidelity dna polymerase/product/New England Biolabs
    Average 99 stars, based on 85 article reviews
    Price from $9.99 to $1999.99
    q5 hot start high fidelity dna polymerase - by Bioz Stars, 2020-04
    99/100 stars
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    99
    New England Biolabs q5 high fidelity dna polymerase
    Q5 High Fidelity Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 430 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 high fidelity dna polymerase/product/New England Biolabs
    Average 99 stars, based on 430 article reviews
    Price from $9.99 to $1999.99
    q5 high fidelity dna polymerase - by Bioz Stars, 2020-04
    99/100 stars
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    95
    New England Biolabs q5 polymerase reagents
    Q5 Polymerase Reagents, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 polymerase reagents/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    q5 polymerase reagents - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

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