q5 polymerase mastermix  (New England Biolabs)


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    Structured Review

    New England Biolabs q5 polymerase mastermix
    Q5 Polymerase Mastermix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 polymerase mastermix/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    q5 polymerase mastermix - by Bioz Stars, 2020-04
    92/100 stars

    Related Products / Commonly Used Together

    luxi homologs

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    Related Articles

    Clone Assay:

    Article Title: Genome sequencing-assisted identification and the first functional validation of N-acyl-homoserine-lactone synthases from the Sphingomonadaceae family
    Article Snippet: Paragraph title: Amplification and cloning of 3 putative AHL synthase genes ... Amplification of the luxI homologs was performed using Q5 polymerase mastermix (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions.

    Amplification:

    Article Title: Genome sequencing-assisted identification and the first functional validation of N-acyl-homoserine-lactone synthases from the Sphingomonadaceae family
    Article Snippet: .. Amplification of the luxI homologs was performed using Q5 polymerase mastermix (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions. .. Approximately 150 ng of the purified PCR amplicons were mixed with 50 ng of pSRKKm vector ( ) and double digested with NheI and NdeI (New England Biolabs, Ipswich, MA, USA) for 1 h. After heat inactivation, the digested products were purified using magnetic beads (Omega Biotek, Norcross, GA, USA) and ligated with Electroligase (New England Biolabs, Ipswich, MA, USA) for 30 min.

    Magnetic Beads:

    Article Title: Genome sequencing-assisted identification and the first functional validation of N-acyl-homoserine-lactone synthases from the Sphingomonadaceae family
    Article Snippet: Amplification of the luxI homologs was performed using Q5 polymerase mastermix (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions. .. Approximately 150 ng of the purified PCR amplicons were mixed with 50 ng of pSRKKm vector ( ) and double digested with NheI and NdeI (New England Biolabs, Ipswich, MA, USA) for 1 h. After heat inactivation, the digested products were purified using magnetic beads (Omega Biotek, Norcross, GA, USA) and ligated with Electroligase (New England Biolabs, Ipswich, MA, USA) for 30 min.

    Purification:

    Article Title: Genome sequencing-assisted identification and the first functional validation of N-acyl-homoserine-lactone synthases from the Sphingomonadaceae family
    Article Snippet: Amplification of the luxI homologs was performed using Q5 polymerase mastermix (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions. .. Approximately 150 ng of the purified PCR amplicons were mixed with 50 ng of pSRKKm vector ( ) and double digested with NheI and NdeI (New England Biolabs, Ipswich, MA, USA) for 1 h. After heat inactivation, the digested products were purified using magnetic beads (Omega Biotek, Norcross, GA, USA) and ligated with Electroligase (New England Biolabs, Ipswich, MA, USA) for 30 min.

    Polymerase Chain Reaction:

    Article Title: Genome sequencing-assisted identification and the first functional validation of N-acyl-homoserine-lactone synthases from the Sphingomonadaceae family
    Article Snippet: Amplification of the luxI homologs was performed using Q5 polymerase mastermix (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions. .. Approximately 150 ng of the purified PCR amplicons were mixed with 50 ng of pSRKKm vector ( ) and double digested with NheI and NdeI (New England Biolabs, Ipswich, MA, USA) for 1 h. After heat inactivation, the digested products were purified using magnetic beads (Omega Biotek, Norcross, GA, USA) and ligated with Electroligase (New England Biolabs, Ipswich, MA, USA) for 30 min.

    Electroporation:

    Article Title: Genome sequencing-assisted identification and the first functional validation of N-acyl-homoserine-lactone synthases from the Sphingomonadaceae family
    Article Snippet: Amplification of the luxI homologs was performed using Q5 polymerase mastermix (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions. .. The ligated products were transformed into A. tumefaciens NTL4 and Escherichia coli JM109 using electroporation.

    Transformation Assay:

    Article Title: Genome sequencing-assisted identification and the first functional validation of N-acyl-homoserine-lactone synthases from the Sphingomonadaceae family
    Article Snippet: Amplification of the luxI homologs was performed using Q5 polymerase mastermix (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions. .. The ligated products were transformed into A. tumefaciens NTL4 and Escherichia coli JM109 using electroporation.

    Plasmid Preparation:

    Article Title: Genome sequencing-assisted identification and the first functional validation of N-acyl-homoserine-lactone synthases from the Sphingomonadaceae family
    Article Snippet: Amplification of the luxI homologs was performed using Q5 polymerase mastermix (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions. .. Approximately 150 ng of the purified PCR amplicons were mixed with 50 ng of pSRKKm vector ( ) and double digested with NheI and NdeI (New England Biolabs, Ipswich, MA, USA) for 1 h. After heat inactivation, the digested products were purified using magnetic beads (Omega Biotek, Norcross, GA, USA) and ligated with Electroligase (New England Biolabs, Ipswich, MA, USA) for 30 min.

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    New England Biolabs q5 hot start high fidelity 2x mastermix
    Q5 Hot Start High Fidelity 2x Mastermix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 hot start high fidelity 2x mastermix/product/New England Biolabs
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    New England Biolabs q5 hotstart hifi pcr mastermix
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    https://www.bioz.com/result/q5 hotstart hifi pcr mastermix/product/New England Biolabs
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    q5 hotstart hifi pcr mastermix - by Bioz Stars, 2020-04
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