q5 polymerase amplification  (New England Biolabs)


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    Structured Review

    New England Biolabs q5 polymerase amplification
    Q5 Polymerase Amplification, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 polymerase amplification/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    q5 polymerase amplification - by Bioz Stars, 2020-03
    86/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Enzymatic control of dioxygen binding and functionalization of the flavin cofactor
    Article Snippet: Genes were sequenced (GATC Biotech) following Q5 polymerase amplification (NEB) to confirm the mutations and then transformed and expressed in Escherichia coli BL21(DE3) cells (Novagen). .. Cells were harvested by centrifugation, lysed by using an Ultrasonifier, and centrifuged at 18,000 × g (relative centrifugal force) for 20 min.

    Amplification:

    Article Title: Enzymatic control of dioxygen binding and functionalization of the flavin cofactor
    Article Snippet: .. Genes were sequenced (GATC Biotech) following Q5 polymerase amplification (NEB) to confirm the mutations and then transformed and expressed in Escherichia coli BL21(DE3) cells (Novagen). .. Cells were harvested by centrifugation, lysed by using an Ultrasonifier, and centrifuged at 18,000 × g (relative centrifugal force) for 20 min.

    Mutagenesis:

    Article Title: Enzymatic control of dioxygen binding and functionalization of the flavin cofactor
    Article Snippet: Paragraph title: Mutagenesis, Expression, and Protein Production. ... Genes were sequenced (GATC Biotech) following Q5 polymerase amplification (NEB) to confirm the mutations and then transformed and expressed in Escherichia coli BL21(DE3) cells (Novagen).

    Expressing:

    Article Title: Enzymatic control of dioxygen binding and functionalization of the flavin cofactor
    Article Snippet: Paragraph title: Mutagenesis, Expression, and Protein Production. ... Genes were sequenced (GATC Biotech) following Q5 polymerase amplification (NEB) to confirm the mutations and then transformed and expressed in Escherichia coli BL21(DE3) cells (Novagen).

    Transformation Assay:

    Article Title: Enzymatic control of dioxygen binding and functionalization of the flavin cofactor
    Article Snippet: .. Genes were sequenced (GATC Biotech) following Q5 polymerase amplification (NEB) to confirm the mutations and then transformed and expressed in Escherichia coli BL21(DE3) cells (Novagen). .. Cells were harvested by centrifugation, lysed by using an Ultrasonifier, and centrifuged at 18,000 × g (relative centrifugal force) for 20 min.

    Plasmid Preparation:

    Article Title: Enzymatic control of dioxygen binding and functionalization of the flavin cofactor
    Article Snippet: Mutations were introduced into the pHIS8- encM vector , which contained the encM ) from Sigma-Aldrich. .. Genes were sequenced (GATC Biotech) following Q5 polymerase amplification (NEB) to confirm the mutations and then transformed and expressed in Escherichia coli BL21(DE3) cells (Novagen).

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    New England Biolabs high fidelity pcr amplification
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